D224769 Anti-DDX47 antibody Cat. No. D224769 Package 25 μl/100 μl/200 μl Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Product overview Description A n ti-DDX47 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human DDX47 Reactivity Human, Mouse Content 0 .6 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification Target information Symbol DDX47 Full name DEAD-box helicase 47 Synonyms RRP3; E4-DBP; HQ0256; MSTP162 Swissprot Q9H0S4 Target Background This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variantsS encodingangon distinct isoforms have been found for Bithis gene. otech Applications Immunohistochemistry Predicted cell location: Nucleus Positive control: Human esophagus cancer Recommended dilution: 50-200 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using D224769(DDX47 Antibody) at dilution 1/80, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:51 kDa Positive control:A172 cell lysate Recommended dilution: 500-2000 Gel: 8%SDS-PAGE Lysate: 40 μg Lane: A172 cell lysate Primary antibody: D224769(DDX47 Antibody) at dilution 1/600 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 1 minute ELISA Sangon Biotech Recommended dilution: 5000-10000 .