bioRxiv preprint doi: https://doi.org/10.1101/2021.02.12.431052; this version posted February 13, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 A nadA mutation confers nicotinic acid auxotrophy in pro-carcinogenic intestinal 2 Escherichia coli NC101 3 Lacey R. Lopez,a Cassandra J. Barlogio,a* Christopher A. Broberg,a• Jeremy Wang,b Janelle C. 4 Arthur,a,c,d# 5 aDepartment of Microbiology and Immunology, The University of North Carolina at Chapel Hill, 6 Chapel Hill, North Carolina, United States of America. 7 bDepartment of Genetics, The University of North Carolina at Chapel Hill, Chapel Hill, North 8 Carolina, United States of America. 9 cCenter for Gastrointestinal Biology and Disease, The University of North Carolina at Chapel 10 Hill, Chapel Hill, North Carolina, United States of America. 11 dLineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, 12 Chapel Hill, North Carolina, United States of America. 13 Running title: Eliminating E. coli micronutrient constraints 14 *Present address: Locus Biosciences, Inc., Durham, North Carolina, United States of America. 15 •Present address: Department of Chemistry, The University of North Carolina at Chapel Hill, 16 Chapel Hill, North Carolina, United States of America 17 #Address correspondence to Janelle C. Arthur,
[email protected] 18 Abstract word count (max 250): 204 words 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.12.431052; this version posted February 13, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder.