CENPH Antibody Cat. No.: 63-840
CENPH Antibody
Formalin-fixed and paraffin-embedded human testis Flow cytometry analysis of Hela cells (bottom histogram) tissue reacted with CENPH Antibody , which was compared to a negative control cell (top histogram). FITC- peroxidase-conjugated to the secondary antibody, conjugated goat-anti-rabbit secondary antibodies were followed by DAB staining. used for the analysis.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
This CENPH antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 136-162 amino acids from the Central region of human CENPH.
TESTED APPLICATIONS: Flow, IHC-P, WB
September 30, 2021 1 https://www.prosci-inc.com/cenph-antibody-63-840.html For WB starting dilution is: 1:1000
APPLICATIONS: For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
PREDICTED MOLECULAR 28 kDa WEIGHT:
Properties
This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: CENPH
Centromere protein H, CENP-H, Interphase centromere complex protein 35, CENPH ALTERNATE NAMES: (HGNC:17268), ICEN35
ACCESSION NO.: Q9H3R5
PROTEIN GI NO.: 74733576
GENE ID: 64946
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric BACKGROUND: heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
September 30, 2021 2 https://www.prosci-inc.com/cenph-antibody-63-840.html ANTIBODIES FOR RESEARCH USE ONLY.
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September 30, 2021 3 https://www.prosci-inc.com/cenph-antibody-63-840.html