CENPH Antibody Cat. No.: 63-840 CENPH Antibody Formalin-fixed and paraffin-embedded human testis Flow cytometry analysis of Hela cells (bottom histogram) tissue reacted with CENPH Antibody , which was compared to a negative control cell (top histogram). FITC- peroxidase-conjugated to the secondary antibody, conjugated goat-anti-rabbit secondary antibodies were followed by DAB staining. used for the analysis. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human This CENPH antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 136-162 amino acids from the Central region of human CENPH. TESTED APPLICATIONS: Flow, IHC-P, WB September 30, 2021 1 https://www.prosci-inc.com/cenph-antibody-63-840.html For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 PREDICTED MOLECULAR 28 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: CENPH Centromere protein H, CENP-H, Interphase centromere complex protein 35, CENPH ALTERNATE NAMES: (HGNC:17268), ICEN35 ACCESSION NO.: Q9H3R5 PROTEIN GI NO.: 74733576 GENE ID: 64946 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric BACKGROUND: heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. September 30, 2021 2 https://www.prosci-inc.com/cenph-antibody-63-840.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 30, 2021 3 https://www.prosci-inc.com/cenph-antibody-63-840.html.