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Degranulation of Human Mast Cells Induces an Endothelial Antigen

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Degranulation of Human Mast Cells Induces an Endothelial Antigen Proc. Natl. Acad. Sci. USA Vol. 86, pp. 8972-8976, November 1989 Medical Sciences Degranulation of human mast cells induces an endothelial antigen central to leukocyte adhesion (tumor necrosis factor/skin/organ culture/inflammation/cytokines) LYNN M. KLEIN, ROBERT M. LAVKER, WENDY L. MATIS, AND GEORGE F. MURPHY Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 Communicated by K. Frank Austen, August 21, 1989 ABSTRACT To understand better the role of mast cell Recent studies suggest (14) that endothelial activation and secretory products in the genesis ofinflammation, a system was associated adhesion of blood leukocytes to endothelial sur- developed for in vitro degranulation ofhuman mast cells in skin faces are mediated by specific glycoproteins inducible on organ cultures. Within 2 hr after morphine sulfate-induced human vascular endothelial cells. One such glycoprotein degranulation, endothelial cells lining microvessels adjacent to [endothelial-leukocyte adhesion molecule 1 (ELAM-1), ref. affected mast cells expressed an activation antigen important 15] has been cloned, sequenced, and is demonstrable by the for endothelial-leukocyte adhesion. Identical results were ob- monoclonal antibody H4/18 (16) exclusively on cytokine- tained when other mast cell secretagogues (anti-IgE, compound stimulated or inflamed vascular endothelium. ELAM-1 and 48/80, and calcium ionophore A23187) were used. Induction of related molecules correlate with endothelial activation and this antigen was abrogated by preincubation with cromolyn with endothelial-dependent mechanisms of neutrophil and sodium, an inhibitor of mast cell secretion, and by antiserum mononuclear cell adhesion to the endothelial cell surface in to tumor necrosis factor a. These findings indicate that de- vitro (17, 18). In organ cultures of human skin, ELAM-1 may granulation ofmast cells activates dermal endothelium through be induced only in endothelium ofpost-capillary venules (19), tumor necrosis factor-dependent mechanisms. This event may the targets of most cutaneous inflammatory reactions. More- be critical to the elicitation phase of cutaneous inflammation. over, expression ofELAM-1 is integral to the evolution ofthe cutaneous delayed hypersensitivity response (20). Undefined Mast cells are resident, granule-containing secretory cells factors released by skin explants into culture supernatants situated about blood vessels of host-environmental inter- are capable of inducing ELAM-1 in target monolayers of large-vessel endothelial cells (10). The recombinant immuno- faces, such as skin and mucosae. Identified more than a regulatory proteins (cytokines) tumor necrosis factor a century ago by Ehrlich (1), it has been hypothesized that mast (TNF-a) and interleukin 1 (IL-1) elicit endothelial activation cells play a central role in the genesis and modulation of and expression of ELAM-1 in vitro (21-23). In the skin, IL-1 cellular inflammatory responses (2-4). For example, de- is produced by epithelial cells, and both IL-1 and TNF-a are layed-type hypersensitivity reactions (DHRs) in mice are produced by monocytes (24). Although it is likely that these elicited preferentially at cutaneous sites enriched in mast and other endogenous cytokines are responsible for induction cells, such as ear and footpad (5). Moreover, pharmacologic of ELAM-1 in vivo, the specific stimuli and cells responsible inhibition of release of mast cell secretory products with the for their release have not been defined. cromolyn-like drug proxicromil inhibits the DHR (6). Simi- To understand the cellular events associated with endoge- larly, in sensitized guinea pigs, the DHR is abrogated by first nous mediator release that triggers endothelial activation and purging mast cells at the challenge site with a local injection endothelial-leukocyte adhesion, we have investigated (25) of the mast cell secretagogue compound 48/80 (7). Further- the sequential ultrastructural and immunophenotypic mor- more, mice that are genetically deficient in mast cells (W/W' phology of the cutaneous DHR in normal human skin. and S1/Slid) have been reported (4) to exhibit diminished Degranulation of mast cells about superficial vessels was the DHR. An unresolved issue in all such studies is whether mast first ultrastructural alteration noted, 4 hr after contact antigen cell degranulation products are capable of primarily trigger- (dinitrochlorobenzene) challenge. Degranulation anteceded ing leukocytic infiltration of the skin or whether mast cells accumulation of inflammatory cells (predominantly T lym- degranulate secondarily as a result of the inflammatory phocytes) by =20 hr. We, therefore, reasoned that endoge- cascade (8, 9). nous mediators crucial for local recruitment of circulating Upon degranulation mast cells release histamine, resulting leukocytes may be liberated as a result of degranulation. In in gaps between adjacent endothelial cells. This enhances this report, we demonstrate that mast cell degranulation in vascular permeability for macromolecules within serum (10- the perivascular space results in rapid, cytokine-dependent 12), although it does not always result in inflammatory cell induction of the endothelial-activation antigen ELAM-1 in migration into the perivascular compartment (8). Mast cells adjacent microvasculature. Moreover, this induction appears also contain heparin, a physiological anticoagulant that in- to be dependent upon endogenous release ofa substance with fluences angiogenesis (13). Neither of these observations, immunologic properties of TNF-a. These findings establish however, provides insight as to how mast cells may be mast cell secretion as a critical link in the development of involved in the initial accumulation of inflammatory cells in inflammatory responses. injured or antigen-stimulated tissue. Such accumulation re- quires adhesion of circulating leukocytes to stimulated or "activated" endothelial cells lining blood vessels, transit of MATERIALS AND METHODS leukocytes across the vascular wall, and directional migra- Organ Culture. Normal neonatal human foreskins were tion of these cells once liberated in the perivascular space. obtained from elective surgical circumcision procedures. In The publication costs of this article were defrayed in part by page charge Abbreviations: ELAM-1, endothelial-leukocyte adhesion molecule payment. This article must therefore be hereby marked "advertisement" 1;TNF-a, tumor necrosis factor a; IL-1, interleukin 1; MS, morphine in accordance with 18 U.S.C. §1734 solely to indicate this fact. sulfate; DHR, delayed-type hypersensitivity reaction. 8972 Downloaded by guest on September 29, 2021 Medical Sciences: Klein et al. Proc. Natl. Acad. Sci. USA 86 (1989) 8973 certain experiments, normal adult human skin obtained as anti-IL-1 at 50, 100, and 200 units/mi. Replicate explants 4-mm punch biopsies from the upper arm was also cultured were also incubated in identical concentrations of rabbit and evaluated (see below) to ensure that findings were not serum to exclude nonspecific interference with mast cell peculiar to the neonate. All procedures were performed mediator effects, and ultrastructure was performed to pre- under sterile conditions. Specimens were immediately im- clude the possibility that antiserum influenced secretagogue- mersed in RPMI 1640 medium (Flow Laboratories, McLean, induced degranulation. Recombinant TNF-a was used, as VA) to which 10% (vol/vol) fetal calf serum, 1% penicillin/ described (19), for control ELAM-1 induction in explants. streptomycin, and 10 mM L-glutamine (GIBCO) had been Immunohistochemistry. For immunohistochemical stain- added. While suspended, foreskin was cut into 1- to 2-mm ing, harvested explants were frozen in OCT compound (Miles pieces and placed in 35-mm cell wells, epidermal surface up. Scientific) cooled in liquid nitrogen and stored at -800C. Sufficient medium (-1000 41) to cover 80% of the explant Frozen specimens were cut in 4-gm cryostat sections, air- surface area (epidermis uncovered) was added (complete dried, fixed in acetone, and reacted for 0.5 hr at 370C with the submersion results in disordered epidermal maturation and monoclonal antibody H4/18.' Staining was accomplished by enhanced epithelial outgrowth). Explants were incubated at a three-step peroxidase-antiperoxidase technique (ref. 27) 370C with humidified 5% C02/95% air. Addition of poly- with 3,3'-diaminobenzidene as the chromogenic substrate. myxin B sulfate (50 ,ug/ml), which neutralizes endotoxins, to All incubation times were identical, and controls and block- this culture system does not influence ELAM-1 induction, ing experiments were evaluated side-by-side with positive and medium alone does not elicit ELAM-1 induction in samples. cultured monolayers of human umbilical vein endothelium Morphological Studies. Routine transmission electron mi- (19). Explant viability in this model has been repeatedly croscopy consisted of fixation of explants for 24 hr at 40C. assessed by ultrastructure, the ability to induce class II Specimens were washed in 0.1 M sodium cacodylate buffer, histocompatibility antigens on the surface of target cell types postfixed in 2% (wt/vol) osmium tetroxide for 1 hr, dehy- (e.g., endothelial cells) with recombinant interferon y (26), drated in graded ethanol solutions to propylene oxide, and and the demonstration of continued constitutive endothelial embedded in Epon. Examination of 1-gm sections (28) reactivity for anti-factor VIII antibody (19). In each culture,
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