Comparing the anterior nare bacterial community of two discrete human populations using Illumina amplicon sequencing.

Item Type Article

Authors Camarinha-Silva, Amélia; Jáuregui, Ruy; Chaves-Moreno, Diego; Oxley, Andrew P A; Schaumburg, Frieder; Becker, Karsten; Wos- Oxley, Melissa L; Pieper, Dietmar H

Citation Comparing the anterior nare bacterial community of two discrete human populations using Illumina amplicon sequencing. 2014, 16 (9):2939-52 Environ. Microbiol.

DOI 10.1111/1462-2920.12362

Journal Environmental microbiology

Download date 09/10/2021 21:30:35

Link to Item http://hdl.handle.net/10033/334759 Supplementary figures and tables

Comparing the anterior nare bacterial community of two discrete human populations using Illumina amplicon sequencing

Amélia Camarinha-Silva1, Ruy Jáuregui1, Diego Chaves-Moreno1, Andrew P.A. Oxley1,2, Frieder Schaumburg3, Karsten Becker3, Melissa L. Wos-Oxley1, Dietmar H. Pieper1*

1Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany; 2Infection Immunology Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany; 3Institute of Medical Microbiology, University Hospital Münster, Münster, Germany *For correspondence: E-mail [email protected]; Tel. (+49) 531 6181 4200; Fax (+49) 531 6181 4499

Summary The anterior nares are an important reservoir for opportunistic pathogens and commensal microorganisms. A barcoded Illumina paired-end sequencing method targeting the 16S rRNA V1-2 hypervariable region was developed to compare the bacterial diversity of the anterior nares across distinct human populations (volunteers from Germany vs a Babongo Pygmy tribe, Africa). Of the 251 phylotypes detected, 231 could be classified to the genus level and 109 to the species level, including the unambiguous identification of the ubiquitous Staphylococcus aureus and Moraxella catarrhalis. The global bacterial community of both adult populations revealed that they shared 85% of the phylotypes, suggesting that our global bacterial communities have likely been with us for thousands of years. Of the 34 phylotypes unique to the non-westernized population, most were related to members within the suborder Micrococcineae. There was an even more overwelming distinction between children and adults of the same population, suggesting a progression of a childhood community of high diversity comprising species of Moraxellaceae and Streptococcaceae to an adult community of lower diversity comprising species of Propionibacteriaceae, Clostridiales Incertae Sedis XI, Corynebacteriaceae and Staphylococcaceae. Thus, age was a stronger factor for accounting for differing bacterial assemblages than the origin of the human population sampled.

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Fig S1. Correlation co-efficients after comparing aggregated data matrices. The correlation co-efficient (rho) derived when each of the genus-, family-, order-, class- and phylum-level data matrices were compared to the original species-level matrix, as determined by using the RELATE routine in PRIMER.

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Fig. S2. Sampling effort across each sample, individual-based rarefaction curves. Rarefaction curves portraying the number of resolved phylotypes against sampling depth of each sample within the (A) non-westernized population and (B) westernized population.

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Fig. S3. Sampling effort across each population, species accumulation curves. Estimators of species richness are the total number of all species (Sobs) and the Chao 2 estimator of true richness. Plotted values are the mean +/- standard deviation of 999 permutations. (A) curves representing both westernized and non-westernized populations, (B) curves representing both non- westernized adults and children.

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Fig. S4. Maximum-likelihood tree depicting the taxonomic breadth of the 251 phylotypes detected from the anterior nares. The taxonomic breadth of westernized adults () and non- westernized adults () and non-westernized children (), as represented by complete or near complete 16S rRNA gene sequences from their closest taxonomic relatives available from the SILVA database. GenBank accession numbers are given after each strain name. Branch support values were calculated from 1000 bootstrap re-samplings (values > 85% are given at the nodes). Scale bar represents 5% nucleotide sequence divergence.

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Fig. S5. Comparing the relative abundance and prevalence of selected phylotypes in the anterior nare bacterial communities. (A) Kocuria sp., (B) Kocuria marina and (C) Kocuria koreensis observed in westernized and non-westernized adults.

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Fig. S6. Non-metric multidimensional scaling (nMDS) plot comparing the global bacterial community structure of 190 human anterior nares. (A) Community structures across 147 adult volunteers (non-westernized () n=55, westernized () n=92). (B) Community structures across 92 non-westernized volunteers (non-westernized adults () n=55, non-westernized children () n=43). For each phylotype the amount of sequence reads was standardized (%) but untransformed prior to the use of the Bray-Curtis similarity algorithm. While 2D stress values of 0.18 and 0.19 indicate some stress on the plots, it is deemed acceptable considering that so many samples are being ordinated.

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Fig. S7. Non-metric multidimensional scaling (nMDS) plot with superimposed bubbles. (A,C,E,G) Superimposed bubbles onto the ordination plot of Fig. S6A ordinating both adult populations (where westernized adults are denoted by an askerisk and non-westernized adults denoted by a hash). (B,D,F,H) Superimposed bubbles onto the ordination plot of Fig. S6B ordinating non-westernized children and adults (where non-westernized adults are denoted by a hash and non-westernized children denoted by a cross). Bubbles represent the relative abundance of (A-B) C. accolens (PT1-1), (C-D) P. acnes (PT3-1), (E-F) S. epidermidis/S. capitis/S. caprae (PT38-1) and (G-H) Peptoniphilus sp. (PT44-1). 8

Fig. S8. Comparing the relative abundance and prevalence of selected phylotypes in the anterior nare bacterial communities. (A) S. aureus, (B) M. lacunata/ M. nonliquefaciens and (C) M. catarrhalis observed in females and males in the non-westernized adults, (D) M. lacunata/ M. nonliquefaciens and (E) M. catarrhalis observed in females and males in the westernized adults.

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Fig. S9. Non-metric multidimensional scaling (nMDS) plot with superimposed bubbles. (A,C,E) Superimposed bubbles onto the ordination plot of Fig. S6A ordinating both adult populations (where westernized adults are denoted by an askerisk and non-westernized adults denoted by a hash). (B,D,F) Superimposed bubbles onto the ordination plot of Fig. S6B ordinating non-westernized children and adults (where non-westernized adults are denoted by a hash and non-westernized children denoted by a cross). Bubbles represent the relative abundance of (A-B) Streptococcus pneumoniae/S. mitis (PT59-1), (C-D) Dolosigranulum pigrum (PT66-1), (E-F) Dolosigranulum sp. (PT72-1).

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Fig. S10. Non-metric multidimensional scaling (nMDS) plot with superimposed bubbles. (A,C,E) Superimposed bubbles onto the ordination plot of Fig. S6A ordinating both adult populations (where westernized adults are denoted by an askerisk and non-westernized adults denoted by a hash). (B,D,F) Superimposed bubbles onto the ordination plot of Fig. S6B ordinating non-westernized children and adults (where non-westernized adults are denoted by a hash and non-westernized children denoted by a cross). Bubbles represent the relative abundance of (A-B) Staphylococcus aureus (PT26-1), (C-D) Moraxella lincolnii (PT98-1), (E-F) Moraxella catarrhalis (PT123-1).

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Fig. S11. Ecological biodiversity indices of the anterior nare bacterial communities of non- westernized adults and children and westernized adults. The indices presented here are: total phylotypes (S), Shannon diversity (H’), Pielou’s evenness (J’), Simpson index (1-lambda), average Taxonomic Distinctness (delta+) and variation in Taxonomic Distinctness (lambda+).

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Table S1. Nucleotide sequences of all 251 phylotypes determined using Illumina-based amplicon deep-sequencing.

Phylotype Sequence

PT1-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-2 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT1-3 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAAGGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-5 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAAGGGCCGACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-6 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGTGAATGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-7 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAAGGGCCTACCAAGGGGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-9 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCGACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-10 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGGAAGGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-11 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAAGTGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-12 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGACACGGCCCAA

PT1-13 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGGGGCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-14 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAAGGGCCTCCCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-15 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGGAATGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-8 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGTCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGACACGGCCCAG

PT1-16 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTCCCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-18 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGGAATGGCCGACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-19 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAAGGGCCTACCAAGGCGGCGGCGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-20 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCGACCAAGGGGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-21 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT1-22 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAGTGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-23 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCGCCCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-24 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTCACAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT1-25 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTCCCAAGGGGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT2-1 GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCCAGCTTGCTGGGGTGCTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTGCGGCCACATTGGGACTGAGATACGGCCCAG

PT3-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTTTGTGGGGTGCTCGAGTGGCGAACG-GGGTAGTGGCTTACCAAGGCTTTGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT3-3 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTTTGTGGGGTGCTCGAGTGGCGAACG-GGGTAGTGGCTTACCAAGGCTTTGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

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Phylotype Sequence

PT3-4 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTTTGTGGGGTGCTCGAGTGGCGAACG-GGGTAGGTGCTTACCAAGGCTTTGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT3-5 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTTTGTGGGGTGCTCGAGTGGCGAACG-GGGTAATGGCTTACCAAGGCTTTGACGGGTAGTCGGCCTGAGAGGGTGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT3-6 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTTTGTGGGGTGCTCGAGTGGCGAACG-GGTGAGTGGCTTACCAAGGCTTTGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT3-7 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTTTGTGGGGTGCTCGAGTGGCGAACG-GGGTATGGGCTTACCAAGGCTTTGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT4-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGATGGAAGCTTGCTTCTATCAATCTTAGTGGCG-GGGTAACGGCCCACCAAGGCGATGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGGACTGAGACACGGCCCAG

PT5-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATTTAGTAAACAGAAGCCTCGGTGGAAGATTACTAAT-AGATAACAGCCCACCAAGGCGACGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGAACTGAGACACGGTCCAA

PT6-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAGATTATTAGACTTCTTCGGAATGAAAATAATTGG-AGAAAAAAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT8-1 GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAGATACGAGAGCTTGCTCTTGTAAATCTTAGTGGCA-GGGTAACGGCCTACCAAGACGATGATCAATAGCCGGTCTGAGAGGATGAACGGCCACACTGGAACTGAGACACGGTCCAG

PT9-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTTAGTAGACTTCTTCGGAAAGACACTAAGCGG-AGATAAAAGCTCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT10-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGCTTGGTGCTTGCACCGAGCGGATGAGTTGCGAA-AGGTAACGGCTCACCAAGGCAACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT12-1 GATGAACGCTGACAGAATGCTTAACACATGCAAGTCTTTGGCGAAGCTGTGCTTGCACAGCCTAGCCAAGGCGGACGGGT-GGGTAACGGCCCACCAAGGCGATGATAGGTAGCCGGCCTGAGAGGGTGGACGGCCACAAGGGGACTGAGATACGGCCCTT

PT13-1 GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCAGGGTAACATGAGGAAAGCTTGCTTTCCTTGATGACGACTGG-GGGTAACGGCCCACCAAGGCTACGATCAGTAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAA

PT15-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCCCTTGTGGGGTACTCGAGTGGCGAACGGGT-GGGTAGTGGCTCACCAAGGCTTTGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT16-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATTTAGTAGACAGAAGCTTCGGTGGAAGATTACTAAT-AGATAACAGCCCACCAAGGCGACGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGAACTGAGACACGGTCCAA

PT17-1 GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCAGGGTAACGTGAGGGAAGCTTGCTTCCCTTGACGACGACTGG-GGGTAACGGCCCACCAAGGCGACGATCAGTAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAA

PT19-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGATAAGGAGCTTGCTCCTTTGACGTTAGCGGCGGA-AGGTAACGGCTTACCAAGGCAACGATACGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAG

PT20-1 ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCAGCACAGAGAAGCTTGCTTCTTGGGTGGCGAGTGGCG-GGGTAAAGGCCTACCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT22-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAGGAGCTTGCTCTTCTGGATGAGTTGCGAACG-GGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT22-2 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAGGAGCTTGCTCTTCTGGATGAGTTGCGAACG-AGGTAACGGCTCACCAAGGCAACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT24-1 GATGAACGCTAGCGATAGGCTTAACACATGCAAGTCGAGGGGTAACGTGTTGGAAGCTTGCTTCCGATGACGACGACCGG-AGGTAACGGCTCACCAAGCCAACGATAGGTAGGGGTGCTGAGAGGCAGATCCCCCACATTGGGACTGAGACACGGCCCAA

PT25-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAGAGCGACCGGTGCTTGCACTGGTCAATCTAGTGGCGAA-AGGTAACGGCTCACCAAGGCCGTGATGCATAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT26-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACGGACGAGAAGCTTGCTTCTCTGATGTTAGCGGCGGA-AGGTAACGGCTTACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAG

PT27-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCACCAGCTTGCTGGTGTGGATGAGTGGCGAACG-AGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT28-1 GACGAACGCTGGCGGCGCGCTTAACACATGCAAGTCGAGCGATGAAATCTTGACAGATCCCTTCGGGGTGAAGATAAGAT-AGGTAACGGCCCACCAAGGCGACGATGGGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGAACTGAGAAACGGTCCAA

PT31-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATTTAGTAGACAGAAACCTCGGTGGAAGATTACTAAT-AGATAACAGCCCACCAAGGCGACGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGAACTGAGACACGGTCCAA

PT32-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTAAGGCTCCAGCTTGCTGGGGTACACGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGACACGGCCCAG

PT35-1 GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCCAGCTTGCTGGGTGGATTAGTGGCGAACGGG-AGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

14

Phylotype Sequence

PT36-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGAGGAGCTTGCTCTTCTTGGATGAGTTGCGAA-GGGTAACGGCTCACCAAGGCAACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT36-2 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGAGGAGCTTGCTCTTCTTGGATGAGTTGCGAA-AGGTAATGGCTCACCTAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT36-3 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGAGGAGCTTGCTCTTCTTGGATGAGTTGCGAA-AGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT37-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTCTGCTTGCAGGGGTGCTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGTCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT38-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGACGAGGAGCTTGCTCCTCTGACGTTAGCGGCGGA-AGGTAACGGCTTACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAG

PT38-2 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGACGAGGAGCTTGCTCCTCTGACGTTAGCGGCGGA-GGGTAACGGCTTACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAG

PT39-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGAGATGAGGTGCTTGCACCTTATCTTAGCGGCGGACGGG-GGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT40-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCTTGGTGCTTGCACTGGGTGGATGAGTGGCGAA-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT40-2 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCTTGGTGCTTGCACTGGGTGGATGAGTGGCGAA-GGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT41-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTTAAATGATTTCTTCGGAATGAACTTAAGTGG-AGAAAAAAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT43-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCTGTGCTTGCACAGTGGATTAGTGGCGAACGGG-AGGTAGTGGCTCACCAAGGCGATGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT44-1 GACGAACGCTGGCGGCGCGCTTAACACATGCAAGTCGAGCGATGAAACTTTAACAGAACCCTTCGGGGCGAAGATAAAGT-GAGTAAAAGCCTACCAAGGCGACGATGGGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGAACTGAGAAACGGTCCAA

PT45-1 ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGGGGAGGAGCTTGCTTCTCTGCCGGCGAGTGGCG-AGGTAAAGGCTCACCAAGGCGAAGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT46-1 GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCAAGCTTGCTTGGGTACTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGTCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT48-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAGGAGCTTGCTCTTTCCGGATGAGTTGCGAAC-AGGTAACGGCTCACCAAGGCAACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT49-1 ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGCAGCACGGGAGCAATCCTGGTGGCGAGTGGCGAACGGG-GGGTAAAGGCCTACCAAGGCGACGATCAGTAGCTGGTCTGAGAGGACGATCAGCCACACTGGGACTGAGACACGGCCCAG

PT50-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCTAGCTTGCTAGGTGGATTAGTGGCGAACGGG-AGGTAACGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT50-2 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCTAGCTTGCTAGGTGGATTAGTGGCGAACGGG-AGGTAATGGCTTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT51-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAGCAGGAGAAAGCTTGCTTTCTTGCTGACGAGTGGCG-GGGTAAAGGCCTACCAAGCCTGCGATCTCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAG

PT51-2 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAGCAGGAGAAAGCTTGCTTTCTTGCTGACGAGTGGCG-GGGTAAGGGCCTACCAAGCCTGCGATCTCTAGCTGGTCTGAGAGGATGGCCAGCCACACTGGAACTGAGACACGGTCCAG

PT52-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCAGTGCTTGCACTGGTACTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGTCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT53-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTTTACTGACTTCTTCGGAAAGATTTAAAGCGG-AGAAAAAAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT54-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTTAAAAGATTTCTTCGGAATGAACTTAAGTGA-AGATAACAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT55-1 GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCTCCTGCTTGCAGGGGTACTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGTCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT59-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAGGAGCTTGCTTCTCTGGATGAGTTGCGAACG-GGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT59-2 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAGGAGCTTGCTTCTCTGGATGAGTTGCGAACG-AGGTAACGGCTCACCAAGGCAACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT60-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCTCCAGCTTGCTGGAGTACTCGAGTGGCGAACG-GGGTGATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGATCGGCCACATTGGGACTGAGATACGGCCCAG

15

Phylotype Sequence

PT61-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGGTAAGGCCCTTTCGGGGGTACACGAGTGGCGAACGGGTG-GGGTAGTGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT62-1 GACGAACGCTGGCTGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCCTGGTGCTTGCACCGGGTGGATGAGTGGCGAA-AGGTAATGGCTCACCAAGACGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT63-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTTTAATGAACCCTTCGGGGAGAATTAAAGCGG-AGATAAAAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT64-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCCTTTGGGGGTACTCGAGTGGCGAACGGGTG-GGGTAGTGGCTTACCAAGGCTTTGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT65-1 ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGAGGAGAAGCTTGCTTCTCTGTCGGCGAGTGGCG-GGGTAATGGCTCACCAAGGCAATGATCCGTAGCGGGTCTGAGAGGACGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT66-1 GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGATGATATCACTGCTTGCAGTGATTGATTAGTGGCGAACG-AGGTAATGGCTTACCAAGGCAGTGATACGTAGCCGACTTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT68-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTTGAGCGCTGAAGGTTGGTACTTGTACCAACTGGATGAGCAGCGAA-AGGTAAAGGCTCACCAAGGCGATGATACATAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT69-1 AGTGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGATGAAGTCCTAGCTTGCTAGGATGGATTAGTGGCGCACG-AGGTAATGGCTTACCAAGGCTATGACGCGTAACTGGTCTGAGAGGATGATCAGTCACATTGGAACTGAGACACGGTCCAA

PT70-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTGAAGGGATCAGCTTGCTGGTTCTGGATGAGTGGCGAA-GGGTGATGGCCTACCAAGGCGGTGACGGGTAGCCGGCCTGAGAGGGTGGACGGTCACACTGGGACTGAGACACGGCCCAG

PT71-1 ATTGAACGCTAGCGGGATGCTTTACACATGCAAGTCGAACGGCAGCGCGAGAGAGCTTGCTCTCTTGGCGGCGAGTGGCG-GGGTAAAGGCTCACCAAGGCAACGATCCGTAGCTGGTTTGAGAGGACGACCAGCCACACTGGGACTGAGACACGGCCCAG

PT72-1 GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGATGACGTCACTGCTTGCAGTGACTGATTAGTGGCGAACG-AGGTAATGGCTTACCAAGGCAGTGATACGTAGCCGACTTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT72-2 GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGATGACGTCACTGCTTGCAGTGACTGATTAGTGGCGAACG-AGGTAATGGCTTACCAAGGCAGTGATACGTAGCCGACTTGAGAGGGTGATCGGCCACATTGGGACTGAGATACGGCCCAG

PT73-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCACAGTTTATACCGTAGCTTGCTACACCATAGACTGTGA-AGGTAACGGCTCACCTAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT74-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTTTATAGAATTCTTCGGAAGGAAATAAAGAGG-AGATAAAAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT76-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATGAAGTCTAGCTTGCTAGACGGATTAGTGGCGAACGGG-GGGTAAAGGCCTACCAAGGCGACGATCTGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAG

PT77-1 ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGACGGGAGCTTGCTCCTTGATTCAGCGGCGGACGGG-GGGTAATGGCTCACCAAGGCGACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAG

PT79-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAAAGAAGCTTGCTTCTTTGATGACGAGTGGCG-AGGTAAAGGCTCACCAAGCCGACGATCTCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGGACTGAGACACGGCCCAG

PT80-1 GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGGCATTGAGTGCTTGCACTCTTTGGACGTCGACC-GGGTAACGGCCCACCAAGGCAACGATCAGTAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAA

PT81-1 ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCAGCGGGGTAGTGCTTGCACTACTGCCGGCGAGTGGCG-GGGTAAAGGCCTACCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT82-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTTTTCGGAGGTACTCGAGTGGCGAACGGGTG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT83-1 GATGAACGCTGACAGAATGCTTAACACATGCAAGTCAACTTGAACTTCGGTTTGGGTGGCGGACGGGTGAGTAACGCGTA-AGGTAACGGCTCACCAAGGCGATGATGGGTAGCCGGCCTGAGAGGGTGATCGGCCACAAGGGGACTGAGACACGGCCCTT

PT84-1 GATGAACGCTAGCGATAGGCTTAACACATGCAAGTCGAGGGGCAGCATGGTCTTAGCTTGCTAAGACTGATGGCGACCGG-AGGTAACGGCTCACCAAGGCGACGATGGGTAGGGGAACTGAGAGGTTGAACCCCCACACTGGTACTGAGACACGGACCAG

PT86-1 GATGAACGCTAGCTATAGGCTTAACACATGCAAGTCGAGGGGCAGCGAATAGATAGCTTGCTATTTATGTCGGCGACCGG-GGGTAACGGCCCACCAAGGCAACGATCAGTAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAA

PT88-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGGAGAGCTTGCTCTTCTGGATGAGTTGCGAACG-GGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT89-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTTAAATGAATTCTTCGGAATGATTTTAATTGG-AGATAACAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT90-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGACGGTGGTGCTTGCACCGCCTGATTAGTGGCGAACG-AGGTGATGGCTCACCAAGACGATGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT90-2 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGACGGTGGTGCTTGCACCGCCTGATTAGTGGCGAACG-AGGTAGTGGCTCACCAAGGCGATGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

16

Phylotype Sequence

PT91-1 GACGAACGCTGGCGGCGCGCTTAACACATGCAAGTCGAGCGATGAAACTTTAACAGAACTCTTCGGAGTGAAGATAAAGT-GAGTAAAAGCCTACCAAGGCGACGATGGGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGAACTGAGAAACGGTCCAA

PT92-1 GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGGAAGAAGCTTGCTTCTTCTGATGGCGACCGG-GGGTAACGGCCCACCTAGCCATCGATCGGTAGGGGTTCTGAGAGGAAGGTCCCCCACACTGGAACTGAGACACGGTCCAG

PT93-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATTTAGTAAACGGAAGCTTCGGTGGAAGATTACTAAT-AGATAACAGCCCACCAAGGCGACGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGAACTGAGACACGGTCCAA

PT94-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCACCAGCTTGCTGGTGTGGATTAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT95-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGTCTGCCTTGAAGATCGGAGTGCTTGCACTCTGTGAAA-GGGTAACGGCCTACCAAGGCGATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT96-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTTGAGCGATGAAGATTGGTGCTTGCACCAATTTGAAGAGCAGCGAA-AGGTAAAGGCTCACCAAGGCGATGATACATAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT97-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAACCGCGACTGAGTGCTTGCACTTGGTCAAGGTGAGTGG-GGGTAACGGCCTACCAAGGCGATGATGCATAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT98-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGAAGAGGTCTAGCTTGCTAGACTGATTAGTGGCGAACGGG-GGGTAAAGGCCTACCAAGGCGACGATCTGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAG

PT100-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATGACTCTCTAGCTTGCTAGAGATGATTAGTGGCGGACG-GGGTAAAGGCCCACCAAGGCGACGATCTGTAACTGGTCTGAGAGGATGATCAGTCACACCGGAACTGAGACACGGTCCGG

PT101-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAAAGGCCACTTCGGTGGTACTCGAGCGGCGAACGGGTG-GGGTAATGGCCTACCATGGCTTCGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT102-1 ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAGAGAGCTTGCTCTCGGGTGACGAGTGGCGGA-GGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAG

PT107-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTCTTCGGAGGTGCTCGAGTGGCGAACGGGTG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT109-1 ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAACAGGAGAAAGCTTGCTTTCTTGCTGACGAGCGGCG-GGGTAAAGGCTCACCTAGGCGACGATCTCTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAG

PT114-1 ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGCAGCACGGACTTCGGTCTGGTGGCGAGTGGCGAACGGG-GGATAAAAGCTTACCAAGCCAACGATCTGTAGCTGGTCTGAGAGGACGATCAGCCACACTGGGACTGAGACACGGCCCAG

PT118-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCGGAACTAACAGATTTACTTCGGTAATGACGTTAGGA-AGGTAAAGGCTTACCAAGGCGATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT120-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTGAAGCTCCAGCTTGCTGGAGTGGATCAGTGGCGAACG-AGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT121-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACTGATTAGAAGCTTGCTTCTATGACGTTAGCGGCGGA-AGGTAACGGCTCACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT123-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGAAGTTAGGAAGCTTGCTTCTGATACTTAGTGGCGGACGG-GGGTAAAGGCCTACCAAGGCGACGATCTGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAG

PT125-1 GACGAACGCTGGCGGCGCGCTTAACACATGCAAGTCGAGCGATGAAACCTTGACAGAACCCTTCGGGGCGAAGACAAAGT-GAGTAAAAGCCTACCAAGGCGACGATGGGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGAACTGAGAAACGGTCCAA

PT127-1 GATGAACGCTGACAGAATGCTTAACACATGCAAGTCTACTTGAATTTGGGTTTTTAACTTAGGTTTGGGTGGCGGACGGG-AGGTAACGGCTCACCAAGGCGATGATGGGTAGCCGGCCTGAGAGGGTGATCGGCCACAAGGGGACTGAGACACGGCCCTT

PT130-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTCTTCGGAGGTACTCGAGTGGCGAACGGGGG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT132-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAAAGGCCCTTCGGGGTACTCGAGCGGCGAACGGGTGAG-AGGTAATGGCTCACCAAGGCTTCGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT136-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTGAAGCCCAGCTTGCTGGGTGGATCAGTGGCGAACGGG-AGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT137-1 GATGAACGCTAGCGATAGGCTTAACACATGCAAGTCGAGGGGCAGCACAAGGTAGCTTGCTATCTGGGTGGCGACCGGCG-AGGTAACGGCTCACCAAGGCAACGATGGGTAGGGGAACTGAGAGGTTTATCCCCCACACTGGTACTGAGACACGGACCAG

PT140-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTAAGGCCCTTCGGGGTACACGAGTGGCGAACGGGTGAG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAG

PT141-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTGAAGGGACTGGTTTTTGCTGGTTCTGGATGAGTGGCG-GGGTGATGGCCTACCAAGGCGGTGACGGGTAGCCGGCCTGAGAGGGTGGACGGTCACACTGGGACTGAGACACGGCCCAG

PT143-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCGAAGCTTGCTTCGGTACTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGACACGGCCCAA

17

Phylotype Sequence

PT143-2 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCGAAGCTTGCTTCGGTACTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT144-1 ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGCAGCTTGCTGCTTTGCTGACGAGTGGCG-GGGTAACGGCTCACCAAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAG

PT145-1 ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCG-GGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAG

PT149-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGACTTTAGCTTGCTAAAGTTGGAAGAGTTGCGAA-AGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT150-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGATGAGAAGCTTGCTTCTCTGATGTTAGCGGCGGA-AGGTAACGGCTTACCAAGGCGACGATACGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAG

PT152-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCTGGGTGCTTGCACCTGGTGGATGAGTGGCGAA-AGGTAATGGCTTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT153-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTTGAACGAGAAATTTATGATTGATTCTTCGGATGAAAGATTAAATG-GGGTAATGGCCTACCATGGCGACGATCAGTAGCCGGATTGAGAGGTTGAACGGCCACACTGGAACTGAGACACGGTCCAG

PT154-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGGAAGGTAGCTTGCTACCGGACCTAGCGGCGGACGGG-GGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT155-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACAGGGATTAGCTTGCTAATCTGCTGACGAGTGGCG-AGGTAAAGGCTGACCAAGCCGACGATCTCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAG

PT156-1 ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAGAGAGCTTGCTCTCGGGTGACGAGCGGCGGA-GGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAG

PT157-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTTAAAAGATTTCTTCGGAATGACCTTAAGTGG-AGATAACAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT160-1 GATGAACGCTAGCTATAGGCTTAACACATGCAAGTCGAGGGGAAACGGCATTAAGTGCTTGCACTTTTTGGACGTCGACC-GGGTAACGGCCCACCAAGGCATCGATCGGTAGGGGTTCTGAGAGGAAGGTCCCCCACATAGGAACTGAGACACGGTCCTA

PT161-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTGCTTTCCCTTGTGGATTGCGGGTGCTCGAG-GGGTAATGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGATACGGCCCAG

PT162-1 GATAAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTTAGTTACTTTCTTCGGAAAGAAATTAAGTGA-AGATAAAAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAA

PT163-1 GACGAACGCTGGCTGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCCCGAAGCTTGCTTCGGGTGGATGAGTGGCGAA-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT164-1 GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGGGGGTTGCTTGCAACCCCTGATGGCGACCGG-GGGTAACGGCCCACCGTGGCGACGATCGGTAGGGGTTCTGAGAGGAAGGTCCCCCACACTGGAACTGAGACACGGTCCAG

PT166-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTGAAGCTCAAGCTTGCTTGGGTGGATCAGTGGCGAACG-AGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT168-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGCAGCGAAAGGTGCTTGCACCTTTCAAGCGAGTGGCGA-GGGTAAAGGCCTACCAAGACAATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT168-2 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGCAGCGAAAGGTGCTTGCACCTTTCAAGCGAGTGGCGA-GGGTAAAGGCTTACCAAGACGATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT170-1 GATGAACGCCGGCGGTGTGCCTAATACATGCAAGTCGAACGCGTTGGCCCAATTGATTGACGGTGCTTGCACCTGATTGA-GGGTAACGGCCTACCAAGGCGATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACAATGGGACTGAGACACGGCCCAT

PT171-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTCGCAAGACCCAGTGGCGGACGGGTGAGTAACGCGT-GGGTAACGGCCTACCAAGGCGACGATCGGTAGCTGGTCTGAGAGGACGATCAGCCACACTGGAACTGAGACACGGTCCAG

PT172-1 ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGACAGGAGCTTGCTCCTGAATTCAGCGGCGGACGGG-AGGTAATGGCTCACCAAGGCGACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAG

PT173-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGTGATGGTGCTTGCACTATCACTTAGCGGCGGACGGG-GGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT174-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCGCAGCTTGCTGTGGTGGATTAGTGGCGAACG-GGGTAGTGGCCTACCAAGGCTTTGACGGTTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT175-1 ATTGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAGGAGCTTGCTTCTCTGGATGAGTTGCGAACG-GGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT176-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCTTGCCTAGATGATTTTAGTGCTTGCACTAAATGAAA-AGGTAACGGCTTACCAAGGCAATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT177-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGTTGAAGGAGAAGCTTGCTTCTCTGGATGAGTTGCGAACG-GGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

18

Phylotype Sequence

PT178-1 GACGAACGCTGGCTGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCCGACAGCTTGCTGTTGGTGGATGAGTGGCGAA-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT179-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGGAAAGGCCTCTTTTTGGGGTACTCGAGTGGCGAACGGGT-GGGTAGTGGCCTACCAAGGCGGTGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACATTGGGACTGAGATACGGCCCAG

PT180-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAGCAGGGATTAGCTTGCTAATTTGCTGACGAGTGGCG-GGGTAAAGGCCTACCAAGCCTGCGATCTCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAG

PT181-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTGAAGCTCCAAGCTTGCTTGGGGTGGATCAGTGGCGAA-AGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT182-1 ATTGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGATGACGTCACTGCTTGCAGTGACTGATTAGTGGCGAACG-AGGTAATGGCTTACCAAGGCAGTGATACGTAGCCGACTTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT183-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTTGTGGTTCAACTGATTTGAAGAGCTTGCTCAGATAT-AGGTAATGGCTTACCAAGGCGATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACAATGGGACTGAGACACGGCCCAT

PT184-1 GACGAACGCTGGCTGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCCAGAAGCTTGCTTCTGGTGGATGAGTGGCGAA-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT186-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGATGGCCGTGCTTGCACGGTCGGATTAGTGGCGAACG-AGGTAGTGGCTTACCAAGGCGATGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT188-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCGGTACTTGTATCGTGGATTAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT189-1 GGTGAACGCTAGCGGTGCGCCTAACACATGCAAGTCGAGCGGTATATTTAATTAAGCTTGCTTAAATAAATAGAGAGCGG-AGGTAAAAGCTTACCAAGGCAATGACGGATAACTGGTCTGAGAGGATGATCAGTCACAATGGAACTGAGATACGGTCCAT

PT190-1 GACGAACGCTGGCTGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCCGGGTGCTTGCACCTGGTGGATGAGTGGCGAA-GGGTAAGGGCCCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT191-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGACGAGAAGCTTGCTTCTCTGATTTAGCGGCGGACG-GGGTAACGGCCTACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT192-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTGGAACGCATTGATATCACCGGAGCTTGCTCCATCGATATTAATGA-GGGTAGCGGCCTACCAAGGCACCGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT193-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCCCTTTCGGGGGTGGATGAGTGGCGAACGGGTG-GGGTAGTGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGTCACACTGGGACTGAGACACGGCCCAG

PT194-1 GATGAACGCTGACAGAATGCTTAACACATGCAAATCTATGAAAAGTTAACTAGCTTGCTAGGTAATGGATCATGGTGGAC-GGGTAAAGGCCTACCAAGGCGATGATAGGTAGCCGGCCCGAGAGGGTGAACGGCCACAAGGGGACTGAGATACGGCCCTT

PT197-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCTTTTCCGCCGAACTTCGGTTCATTGGAAAAGAGGA-AGGTAACGGCTCACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT199-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTGAAGCTCCCAGCTTGCTGGGGGTGGATCAGTGGCGAA-AGGTAATGGCTTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT200-1 GATGAACGCTAGCGGGAGGCTTAACACATGCAAGCCGAGGGGTATTTAATTTTCGGATTAGAGAGACCGGCGAACGGGTG-GTGTAGAGGACTACCAAGGCGATGATCTGTAGGGGGCCTGAGAGGGTGAACCCCCACACTGGTACTGAGACACGGACCAG

PT201-1 GATGAACGCTAGCGGGAGGCCTAACACATGCAAGTCGAGCGGGATATGGTAGCTTGCTACCATTGAGAGCGGCGTACGGG-AGGTAACGGCTCACCAAGGCGATGATCTTTAGGGGGCCTGAGAGGGTGATCCCCCACACTGGGACTGAGACACGGCCCAG

PT204-1 GATGAACGCTAGCGGGAGGCTTAACACATGCAAGCCGAGGGGTATAGTTAGCTTGCTAACTTGAGACCGGCGCACGGGTG-AGGTAACGGCTTACCAAGACGATGATCTTTAGGGGGCCTGAGAGGGTGAACCCCCACACTGGTACTGAGACACGGACCAG

PT206-1 GATGAACGCTGACAGAATGCTTAACACATGCAAATCTATGTAAATTAATTATGCTTGCATAAATAAGGAGCATGGTGGAC-GGGTAAAGGCCTACCAAGGCGATGATAGGTAGCCGGCCCGAGAGGGTGAACGGCCACAAGGGGACTGAGATACGGCCCTT

PT209-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAGCTTTATAGACTTCTTCGGAATGAAATAAAGAGG-AGATAATAGCCCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

PT212-1 AGTGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGATAAGATTTCTAGCTTGCTAGAGATTGAATAGTGGCGCA-AGGTAATGGCTTACCAAGGCCATGACGGGTATCCGGCCTGAGAGGGTGAACGGACACACTGGAACTGAGACACGGTCCAG

PT214-1 GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGTTGGTTGCTTGCAACCGATGATGGCGACCGG-AGGTAACGGCTCACCAAGGCATCGATCAGTAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAA

PT222-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAAGGAGCTTGCTCTTTCCGGATGAGTTGCGAA-AGGTAACGGCTCACCAAGGCAACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT226-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGACGTCACTGCTTGCAGTGACTGATTAGTGGCGAACG-AGGTAATGGCTTACCAAGGCAGTGATACGTAGCCGACTTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT228-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCTGGTGCTTGCACTGGTGGATTAGTGGCGAACG-GGGTAGTGGCCTACCATGGCTTTGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

19

Phylotype Sequence

PT231-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCCCAGCTTGCTGGGTGGATGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT233-1 GACGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATGAAGTCTAGCTTGCTAGACGGATTAGTGGCGAACGGG-GGGTAAAGGCCTACCAAGGCGACGATCTGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAG

PT235-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGTAAGGCCCTTCGGGGTACACGAGCGGCGAACGGGTGAG-GGGTGATGGCCTACCAAGGCGACGACGGATAGCCGGCCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT238-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGTAAGGCCCTTTCGGGGGTACACGAGCGGCGAACGGGTG-GGGTGATGGCCTACCAAGGCGGTGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT239-1 GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCTGGTGCTTGCACCGGGTGGATTAGTGGCGAA-AGGTAATGGCTCACCGTGGCGATGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT241-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGGACCAGCTTGCTGGTTTGGATTAGTGGCGAACG-GGGTAGTGGCCTACCATGGCTTTGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT246-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTAAGGCCCCTTCGGGGGTACACGAGTGGCGAACGGGTG-AGGTAACGGCTCACCAAGGCTTCGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGATACGGCCCAG

PT250-1 GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGACCTAGCAATAGGTTGATGGCGACCGGCGCA-GGGTAACGGCCCACCAAGACATCGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAA

PT251-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGAAAGGTAGCTTGCTACCTGACCTAGCGGCGGACGGG-GGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT253-1 GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGTGAAGTGGTGTTTCCAGTGGCGAACGGGTGCGTAATGCG-AGGCAATAGCTCACCAAGGCTACGATCAGTAGTTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACAAGGCCCAG

PT254-1 GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTCTGCTTGCAGGGGTACTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGTCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT261-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGGATGTTGCTTCGGTAACTGACCTAGCGGCGGACGGG-GGGTAAAGGCCTACCAAGGCGACGATCTGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT278-1 GATGAACGCTAGCGGGAGGCCTAACACATGCAAGCCGAGCGGTATTGTTTCTTCGGAAATGAGAGAGCGGCGTACGGGTG-AGGTAACGGCTCACCAAGGCGATGATCTTTAGGGGGCCTGAGAGGGTGATCCCCCACACTGGTACTGAGACACGGACCAG

PT280-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGAGGAGGGAGCTTGCTCCCTCTGATCGAGTGGCGGA-GGGTAACGGCCCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT281-1 GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGAACTTAGCTTGCTAAGTTTGATGGCGACCGG-GGGTAACGGCCCACCAAGCCTTCGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAA

PT282-1 AACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGCGAATGGAGCAATCCTAGTAGAGCGGCGCACGGGTGCGT-GGGTAATGGCCCACCAAGGCGACGACGGGTAGCTGGTCTGAGAGGACGATCAGCCACACTGGAACTGAGACACGGTCCAG

PT317-1 GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGGACGGGAAGTGGTGTTTCCAGTGGCGGACGGGTGAGTAACGCG-AGGCAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAG

PT360-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCAGCTTGCTGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT372-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCTCAGCTTTTGTTGGGTGGATGAGTGGCGAACG-GGGTGATGGCCTACCAAGGCTTTGACGGGTAGCCGGCCTGAGAGGGTGACCGGTCACATTGGGACTGAGATACGGCCCAG

PT403-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTGAAGCTTGCTTTGGGTACTCGAGTGGCGAA-GGGTAATGGCCTACCAAGGCTTCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT438-1 GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTTACTTCGGTAAAGAGCGGCGGACGGGTGAGTAACGC-AGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGATCGGCCACATTGGAACTGAGACACGGTCCAA

PT487-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAAGTTTTTCTGGTGCTTGCACCGGAAAAACTTAGCGGCG-GGGTAAAGGCCTACCAAGGCGACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT488-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAAGTTTTTCTGGTGCTTGCACTAGAAAAACTTAGCGGCG-GGGTAAAGGCCTACCAAGGCGACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT489-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTGGGACGCAAGAGAACACACTGTGCTTGCACACCGTGTTTTCTTGA-AGGTAAGAGCTTACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT490-1 GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGGTCTTAGCTTGCTAAGGCCGATGGCGACCGG-GGGTAACGGCCCACCTAGTCTTCGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAA

PT491-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACCACTTCGGTGGGAAGCGGCGAACGGGTGAGTAACAC-GGGTAAAGGCCTACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGAACGGCCACACTGGGACTGAGACACGGCCCAG

PT493-1 ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGCAGCGGAAAGTAGCTTGCTACTTTGCCGGCGAGCGGCG-GGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAG

20

Phylotype Sequence

PT494-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAACGGATAAAGAGCTTGCTCTTTTGAAGTTAGTGGCGGA-AGGTAATGGCTTACCAAGGCGACGATCCATAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAA

PT496-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCACTCTCGTTTAGATTGAAGGAGCTTGCTCCTGATTGAT-AGGTAAAGGCTCACCAAGACCGTGATGCATAGCCGACCTGAGAGGGTAATCGGCCACACTGGGACTGAGACACGGCCCAG

PT497-1 GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAGCGAAGCACCTTTTTGGAATCTTCGGAGGAAGAGGAGGTGAC-GGGTAACGGCCTACCAAGGCGACGATCAGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGACACGGCCCAG

PT502-1 AGTGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGGAGCTTATAGTAAGCTTGCTTATTATAAGCTTAGTGGCG-GGGTAATGGCTTACCAAGGCTATGACGCATAACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAG

PT503-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACAGGGATTAGCTTGCTAATTTGCTGACGAGTGGCG-GGGTAAAGGCCTACCAAGCCGACGATCTCTAGCTGGTCTGAGAGGATGATCAGCCACACTGGAACTGAGACACGGTCCAG

PT505-1 ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGAAGAGAAGCTTGCTTCTCTGTCGGCGAGTGGCG-AGGTAATGGCTCACCAAGGCGATGATCCGTAGCGGGTCTGAGAGGACGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT506-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGACAAGGAGCTTGCTCCTTTGACGTTAGCGGCGGA-AGGTAACGGCTTACCAAGGCGACGATACGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAG

PT507-1 ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGAGGAGGAGCTTGCTTCTCTGTCGGCGAGTGGCG-AGGTAATGGCTCACCAAGGCGATGATCCGTAGCGGGTCTGAGAGGACGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT508-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGACGAGGAGCTTGCTCCTTTGACGTTAGCGGCGGA-AGGTAACGGCTTACCAAGGCAACGATACGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAG

PT509-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGACGAGGAGCTTGCTCCTCTAACGTTAGCGGCGGA-AGGTAACAGCTTACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAG

PT510-1 ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGAAGAAGTCTAGCTTGCTAGACTGATTAGTGGCGAACGGG-GGGTAAAAGCCTACCAAGGCGACGATCTGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAG

PT511-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTGCTCGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGTCGACGGGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGATACGGCCCAG

PT511-2 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTGCTCGAGTGGCGAACGGG-GGGTAAGGGCCTACCAAGGCGGCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT512-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTCTTCGGAGGTACTCGAGTGGCGAACGGGTG-GGGTAAGGGCCTACCAAGGCGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT512-2 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTCTTCGGAGGTACTCGAGTGGCGAACGGGTG-GGGTAATGGCCGACCAAGGCGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT512-3 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTCTTCGGAGGTACTCGAGTGGCGAACGGGTG-GGGGAATGGCCTACCAAGGCGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT512-4 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTCTTCGGAGGTACTCGAGTGGCGAACGGGTG-GGGTAATGGCCTGCCAAGGCGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT512-5 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTCTTCGGAGGTACTCGAGTGGCGAACGGGTG-GGGTAATGGCCTACCAAGGGGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT513-1 GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTGCTTGCAGGGTACTCGAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGTCGACGGGTAGCCGGCCTGAGAGGGTGTACGGTCACATTGGGACTGAGATACGGCCCAG

PT514-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCTCCAGCTTGCTGGAGTGCTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGATCGGCCACATTGGGACTGAGATACGGCCCAG

PT515-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCCAGCTTGCTGGGTGGATTAGTGGCGAACGGG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT516-1 ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAACACAGAGAGCTTGCTCTCGGGTGACGAGCGGCGGA-GGGTAATGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAG

PT517-1 ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAACGAGGAGAAGCTTGCTTCTCTGTCGGCGAGTGGCG-GGGTAAGGGCTCACCAAGGCAATGATCCGTAGCGGGTCTGAGAGGACGATCCGCCACACTGGGACTGAGACACGGCCCAG

PT518-1 GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCGAAGCTTGCTTCGGTACTCGAGTGGCGAACG-GGGTAATGGCCTACCAAGGCGTCGACGGGTAGCCGGCCTGAGAGGGTGTACGGCCACATTGGGACTGAGATACGGCCCAG

PT519-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGACGGTGGTGCTTGCACCACCTGATTAGTGGCGAACG-AGGTAATGGCTCACCAAGACGATGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT520-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCTTTTCGGAGGTACTCGAGTGGCGAACGGGGG-GGGTAAGGGCCTACCAAGGCGGCGACGGGTAGCCGGACTGAGAGGTTGGTCGGCCACATTGGGACTGAGATACGGCCCAG

PT521-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGATAAGGAGCTTGCTCCTTTGATGTTAGCGGCGGA-AGGTAATGGCTTACCAAGGCAACGATACGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAG

21

Phylotype Sequence

PT522-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAGGAGCTTGCTTCTCCGGATGAGTTGCGAACG-AGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT523-1 GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAAGGAGCTTGCTCTTTCCGGATGAGTTGCGAA-AGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT524-1 GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGCTTAGTGCTTGCACCGAGCGGATGAGTTGCGAA-GGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT525-1 GATGAACGCTGACAGAATGCTTAACACATGCAAGTCTACTTGAACTTCGGTTTGGGTGGCGGACGGGTGAGTAACGCGTA-AGGTAACGGCTCACCAAGGCGATGATGGGTAGCCGGCCTGAGAGGGTGAACGGCCACAAGGGGACTGAGACACGGCCCTT

PT526-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGACGGTGGTGCTTGCACTGCCTGATTAGTGGCGAACG-AGGTAATGGCTCACCAAGGCGATGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT527-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGACGGCCGTGCTTGCACGGTCTGATTAGTGGCGAACG-AGGTGATGGCTCACCAAGACGATGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAG

PT528-1 GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTAAGGCCCTTTCGGGGGTACACGAGTGGCGAACGGGTG-GGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAG

PT529-1 GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGATTTGTAGCAATACAGATTGATGGCGACCGG-GGGTAACGGCCCACCAAACCGACGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGTACTGAGACACGGACCAA

PT531-1 GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAGCTTAATTGATCCCTTCGGGGTGATTTAAAGTGA-AGATAAAAGCTCACCAAGGCAACGATCAGTAGCCGGCTTGAGAGAGTGTACGGCCACATTGGGACTGAGACACGGCCCAG

22

Table S2. Phylogenetic assignment. Description of all 251 phylotypes determined using Illumina-based amplicon deep-sequencing and the RDP database.

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

PT1-1 160 Corynebacterium accolens AJ439346 100 Corynebacterium accolens Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-2 160 Unc. bacterium GQ003864 100 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

Corynebacterium tuberculostearicum PT1-3 160 99 Corynebacterium tuberculostearicum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ438050

PT1-5 160 Corynebacterium accolens AJ439346 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-6 160 Corynebacterium accolens AJ439346 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-7 160 Corynebacterium accolens AJ439346 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

Corynebacterium tuberculostearicum PT1-9 160 100 Corynebacterium tuberculostearicum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ438050

PT1-10 160 Corynebacterium accolens AJ439346 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-11 160 Corynebacterium accolens AJ439346 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-12 160 Corynebacterium auriscanis AJ243820 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

Corynebacterium tuberculostearicum PT1-13 160 99 Corynebacterium tuberculostearicum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ438050

PT1-14 160 Corynebacterium accolens GQ338419 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-15 160 Unc. bacterium GQ000343 100 Corynebacterium tuberculostearicum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-8 160 Corynebacterium variabile AJ222815 100 Corynebacterium variabile Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

Corynebacterium tuberculostearicum PT1-16 160 99 Corynebacterium tuberculostearicum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ438050 Corynebacterium tuberculostearicum PT1-18 160 99 Corynebacterium tuberculostearicum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ438050

PT1-19 160 Corynebacterium accolens AJ439346 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-20 160 Corynebacterium accolens AJ439346 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-21 160 Unc. bacterium JF184547 100 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-22 160 Unc. bacterium FM875046 100 Corynebacterium tuberculostearicum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-23 160 Corynebacterium accolens GQ338419 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-24 160 Corynebacterium accolens GQ338419 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT1-25 160 Corynebacterium accolens GQ338419 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

23

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

Corynebacterium glucuronolyticum PT2-1 160 100 Corynebacterium glucuronolyticum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium X86688

PT3-1 160 Propionibacterium acnes AB042288 100 Propionibacterium acnes Actinobacteria Actinobacteria Actinomycetales Propionibacteriaceae Propionibacterium

PT3-3 160 Propionibacterium acnes AB042288 99 Propionibacterium acnes Actinobacteria Actinobacteria Actinomycetales Propionibacteriaceae Propionibacterium

PT3-4 160 Propionibacterium acnes AB042288 99 Propionibacterium acnes Actinobacteria Actinobacteria Actinomycetales Propionibacteriaceae Propionibacterium

PT3-5 160 Propionibacterium acnes AB042288 99 Propionibacterium acnes Actinobacteria Actinobacteria Actinomycetales Propionibacteriaceae Propionibacterium

PT3-6 160 Propionibacterium acnes AB042288 99 Propionibacterium acnes Actinobacteria Actinobacteria Actinomycetales Propionibacteriaceae Propionibacterium

PT3-7 160 Propionibacterium acnes AB042288 99 Propionibacterium acnes Actinobacteria Actinobacteria Actinomycetales Propionibacteriaceae Propionibacterium

PT4-1 160 Veillonella parvula CP001820 100 Veillonella sp. Firmicutes Clostridia Clostridiales Veillonellaceae Veillonella

PT5-1 160 Unc. Finegoldia sp. GQ179678 100 Finegoldia magna Firmicutes Clostridia Clostridiales IncertaeSedisXI Finegoldia

PT6-1 160 Unc. bacterium FM875546 100 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

Negativicoccus succinicivorans PT8-1 160 100 Negativicoccus succinicivorans Firmicutes Clostridia Clostridiales Veillonellaceae unclassifiedVeillonellaceae HQ264056

PT9-1 160 Unc. bacterium GQ055471 100 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

PT10-1 160 Streptococcus parasanguinis HM596296 99 Streptococcus parasanguinis Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus

PT12-1 160 Leptotrichia wadei AY029802 100 Leptotrichia wadei Fusobacteria Fusobacteria Fusobacteriales Leptotrichiaceae Leptotrichia

PT13-1 160 Prevotella timonensis DQ518919 100 Prevotella timonensis Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella

PT15-1 160 Propionibacterium sp. Y17821 100 Propionibacterium sp. Actinobacteria Actinobacteria Actinomycetales Propionibacteriaceae Propionibacterium

PT16-1 160 Finegoldia magna AB640691 100 Finegoldia magna Firmicutes Clostridia Clostridiales IncertaeSedisXI Finegoldia

PT17-1 160 Prevotella buccalis AB547676 100 Prevotella buccalis Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella

Staphylococcus warneri/Staphylococcus PT19-1 160 Staphylococcus warneri L37603 100 Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus lugdunensis / Staphylococcus auricularis Neisseria meningitidis alpha14 Neisseria meningitidis / Neisseria PT20-1 160 100 Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Neisseria AM889136 zoodegmatis Streptococcus cristatus/Streptococcus PT22-1 160 Streptococcus cristatus AY188347 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus infantis Streptococcus cristatus/Streptococcus PT22-2 160 Streptococcus infantis AY485603 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus infantis

PT24-1 160 Porphyromonas bennonis AB588020 99 Porphyromonas bennonis Bacteroidetes Bacteroidia Bacteroidales Porphyromonadaceae Porphyromonas

PT25-1 160 Granulicatella adiacens FR822389 100 Granulicatella sp. Firmicutes Bacilli Lactobacillales Carnobacteriaceae Granulicatella

PT26-1 160 Staphylococcus aureus D83355 100 Staphylococcus aureus Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus

24

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

PT27-1 160 Kocuria palustris FR691399 100 Kocuria sp. Actinobacteria Actinobacteria Actinomycetales Micrococcaceae Kocuria

PT28-1 160 Unc. bacterium AY958797 100 Peptoniphilus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Peptoniphilus

PT31-1 160 Finegoldia magna AB109772 100 Finegoldia magna Firmicutes Clostridia Clostridiales IncertaeSedisXI Finegoldia

Corynebacterium amycolatum / PT32-1 160 Corynebacterium amycolatum X82057 100 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium Corynebacterium freneyi

PT35-1 160 Micrococcus luteus type strain AJ536198 100 Micrococcaceae bacterium Actinobacteria Actinobacteria Actinomycetales Micrococcaceae

PT36-1 160 Streptococcus sp. oral taxon HM596298 100 Streptococcus sp. Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus

Streptococcus salivarius / Streptococcus PT36-2 160 Streptococcus salivarius AY188354 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus thermophilus Streptococcus salivarius / Streptococcus PT36-3 160 Streptococcus sanguinis AF003928 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus sanguinis

PT37-1 160 Unc. bacterium GQ002688 100 Uncultured Actinomycetales bacterium Actinobacteria Actinobacteria Actinomycetales

Staphylococcus epidermidis / PT38-1 160 Staphylococcus epidermidis AE015929 100 Staphylococcus capitis / Staphylococcus Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus caprae Staphylococcus epidermidis / PT38-2 160 Unc. bacterium GQ053099 100 Staphylococcus capitis / Staphylococcus Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus caprae PT39-1 160 Acinetobacter junii Z93438 100 Acinetobacter junii Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Acinetobacter

PT40-1 160 Kocuria marina AY211385 100 Kocuria marina Actinobacteria Actinobacteria Actinomycetales Micrococcaceae Kocuria

PT40-2 160 Kocuria rhizophila AP009152 100 Kocuria rhizophila Actinobacteria Actinobacteria Actinomycetales Micrococcaceae Kocuria

PT41-1 160 Anaerococcus octavius Y07841 100 Anaerococcus octavius Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

PT43-1 160 Dermabacter hominis FJ200385 100 Dermabacter hominis Actinobacteria Actinobacteria Actinomycetales Dermabacteraceae Dermabacter

PT44-1 160 Peptoniphilus sp. gpac121 AM176529 100 Peptoniphilus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Peptoniphilus

PT45-1 160 Unc. bacterium GQ080908 100 Neisseriaceae bacterium PT77 Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae

Corynebacterium riegelii / Corynebacterium PT46-1 160 Corynebacterium riegelii Y14651 100 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium tuscaniense

PT48-1 160 Unc. bacterium HM247068 100 Streptococcus sp. Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus

PT49-1 160 Cupriavidus sp. AB438047 99 Cupriavidus sp./Ralstonia sp. Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Cupriavidus/Ralstonia

PT50-1 160 Rothia mucilaginosa AP011540 100 Rothia mucilaginosa Actinobacteria Actinobacteria Actinomycetales Micrococcaceae Rothia

PT50-2 160 Rothia dentocariosa M59055 100 Rothia dentocariosa Actinobacteria Actinobacteria Actinomycetales Micrococcaceae Rothia

PT51-1 160 Haemophilus influenzae AY613454 100 Haemophilus influenzae Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Haemophilus

25

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

PT51-2 160 Haemophilus sp. DQ404358 100 Haemophilus influenzae Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Haemophilus

Corynebacterium aurimucosum PT52-1 160 100 Corynebacterium aurimucosum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AY271720

PT53-1 160 Unc. bacterium GQ087145 100 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

PT54-1 160 Unc. bacterium JF128314 100 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

Corynebacterium timonense / PT55-1 160 Corynebacterium sp. X81904 100 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium Corynebacterium genitalium Streptococcus pneumoniae / Streptococcus PT59-1 160 Streptococcus pneumoniae AF003930 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus mitis Streptococcus pneumoniae / Streptococcus PT59-2 160 Streptococcus oralis FR720602 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus mitis/oralis

PT60-1 160 Unc. bacterium GQ049759 100 Uncultured Actinomycetales bacterium Actinobacteria Actinobacteria Actinomycetales UnclassifiedActinomycetales

PT61-1 160 Propionibacterium granulosum AJ003057 100 Propionibacterium granulosum Actinobacteria Actinobacteria Actinomycetales Propionibacteriaceae Propionibacterium

PT62-1 160 Brevibacterium antiquum AY243344 100 Brevibacterium casei/antiquum Actinobacteria Actinobacteria Actinomycetales Brevibacteriaceae Brevibacterium

PT63-1 160 Anaerococcus sp. HM587319 100 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

Propionibacterium propionicum PT64-1 160 100 Propionibacterium propionicum Actinobacteria Actinobacteria Actinomycetales Nocardiaceae Gordonia AJ003058

PT65-1 160 Unc. bacterium JF228735 100 uncultured Neisseriaceae bacterium PT75/77 Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae

PT66-1 160 Dolosigranulum pigrum X70907 100 Dolosigranulum pigrum Firmicutes Bacilli Lactobacillales Carnobacteriaceae Dolosigranulum

PT68-1 160 Lactococcus lactis AB100804 100 Lactococcus lactis subsp. lactis Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus

PT69-1 160 Campylobacter ureolyticus FN401323 100 Campylobacter-ureolyticus Proteobacteria Epsilonproteobacteria Campylobacterales Campylobacteraceae Campylobacter

Actinomyces naeslundii / Actinomyces PT70-1 160 Actinomyces naeslundii AJ234050 100 Actinobacteria Actinobacteria Actinomycetales Actinomycetaceae Actinomyces viscosus

PT71-1 160 Alcaligenes faecalis AY662683 100 Alcaligenes faecalis subsp. faecalis Proteobacteria Betaproteobacteria Burkholderiales Alcaligenaceae Alcaligenes

PT72-1 160 Unc. bacterium GQ080513 100 Dolosigranulum sp. Firmicutes Bacilli Lactobacillales Carnobacteriaceae Dolosigranulum

PT72-2 160 Unc. bacterium JF179825 99 Dolosigranulum sp. Firmicutes Bacilli Lactobacillales Carnobacteriaceae Dolosigranulum

Streptococcus gordonii / Streptococcus PT73-1 160 Streptococcus gordonii AF003931 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus anginosus

PT74-1 160 Unc. bacterium GQ020166 94 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

Moraxella lacunata/Moraxella PT76-1 160 Moraxella lacunata D64049 100 Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Moraxella nonliquefaciens Pseudomonas putida / Pseudomonas PT77-1 160 Pseudomonas putida CP000712 100 Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas entomophila

PT79-1 160 Haemophilus parainfluenzae AY362908 100 Haemophilus parainfluenzae Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Haemophilus

26

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

PT80-1 160 Prevotella melaninogenica AY323525 100 Prevotella melaninogenica Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella

PT81-1 160 Neisseria sp. oral taxon GQ131417 100 Kingella potus / Neisseria sp. Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Neisseria/Kingella

Corynebacterium propinquum/ PT82-1 160 Corynebacterium propinquum X84438 100 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium Corynebacterium pseudodiphtheriticum Fusobacterium nucleatum subsp. Fusobacterium russii / Fusobacterium PT83-1 160 100 Fusobacteria Fusobacteria Fusobacteriales Fusobacteriaceae Fusobacterium nucleatum FJ471640 nucleatum

PT84-1 160 Porphyromonas sp. oral taxon GU409235 100 Porphyromonas sp. Bacteroidetes Bacteroidia Bacteroidales Porphyromonadaceae Porphyromonas

PT86-1 160 Prevotella bivia AB547673 100 Prevotella bivia Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella

Streptococcus cristatus / Streptococcus PT88-1 160 Streptococcus cristatus AJ853835 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus infantis

PT89-1 160 Unc. bacterium GQ011956 100 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

Brachybacterium paraconglomeratum PT90-1 160 100 Brachybacterium paraconglomeratum Actinobacteria Actinobacteria Actinomycetales Dermabacteraceae Brachybacterium AJ415377

PT90-2 160 Brachybacterium sp. HQ845004 100 Brachybacterium sp. Actinobacteria Actinobacteria Actinomycetales Dermabacteraceae Brachybacterium

PT91-1 160 Unc. bacterium GQ042101 100 Peptoniphilus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Peptoniphilus

PT92-1 160 Prevotella sp. oral taxon GU413280 100 Prevotella sp. Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella

PT93-1 160 Unc. bacterium AM697048 100 Finegoldia magna Firmicutes Clostridia Clostridiales IncertaeSedisXI Finegoldia

PT94-1 160 Dermacoccus sp. JF905611 100 Dermacoccus sp. Actinobacteria Actinobacteria Actinomycetales Dermacoccaceae Dermacoccus

PT95-1 160 Lactobacillus iners Y16329 100 Lactobacillus iners Firmicutes Bacilli Lactobacillales Lactobacillaceae Lactobacillus

PT96-1 160 Lactocuccus lactis AB100802 100 Lactocuccus lactis subsp. cremoris Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus

PT97-1 160 Unc. bacterium GQ113763 100 Abiotrophia defectiva Firmicutes Bacilli Lactobacillales Aerococcaceae Abiotrophia

PT98-1 160 Moraxella lincolni FR822735 100 Moraxella lincolni Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Moraxella

Moraxella osloensis/ Enhydrobacter PT100-1 160 Enhydrobacter aerosaccus AJ550856 100 Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Enhydrobacter aerosaccus

PT101-1 160 Nocardioides terrigena EF363712 100 Nocardoides sp. Actinobacteria Actinobacteria Actinomycetales Nocardioidaceae Nocardioides

Klebsiella oxytoca / Enterobacter PT102-1 160 Enterobacter cloacae Z96079 100 Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae sp./Pantoea sp. Corynebacterium Corynebacterium pseudodiphtheriticum PT107-1 160 99 propinquum/Corynebacterium Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ439343 pseudodiphtheriticum PT109-1 160 Proteus mirabilis DQ885256 100 Proteus mirabilis Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Proteus

PT114-1 160 Acidovorax sp. AY258065 100 Comamonas sp. Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Comamonas

PT118-1 160 Lactobacillus crispatus AF257097 100 Lactobacillus crispatus Firmicutes Bacilli Lactobacillales Lactobacillaceae Lactobacillus

27

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

PT120-1 160 Janibacter limosus Y08539 100 Janibacter sp. Actinobacteria Actinobacteria Actinomycetales Intrasporangiaceae Janibacter

PT121-1 160 Bacillus flexus AB021185 100 Bacillus flexus Firmicutes Bacilli Bacillales Bacillaceae Bacillus

PT123-1 160 Moraxella catarrhalis U10876 100 Moraxella catarrhalis Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Moraxella

PT125-1 160 Unc. bacterium GQ094081 100 Peptoniphilus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Peptoniphilus

Fusobacterium nucleatum subsp. PT127-1 160 100 Fusobacterium nucleatum Fusobacteria Fusobacteria Fusobacteriales Fusobacteriaceae Fusobacterium nucleatum AJ810277 Corynebacterium pseudodiphtheriticum PT130-1 160 99 Corynebacterium pseudodiphtheriticum Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ439343

PT132-1 160 Nocardioides albus AF004988 100 Nocardoides sp. Actinobacteria Actinobacteria Actinomycetales Nocardioidaceae Nocardioides

PT136-1 160 Unc. soil bacterium EF688360 100 Micrococcineae bacterium Actinobacteria Actinobacteria Actinomycetales Cellulomonadaceae Cellulomonas

PT137-1 160 Porphyromonas sp. oral taxon GU429852 100 Porphyromonas sp. Bacteroidetes Bacteroidia Bacteroidales Porphyromonadaceae Porphyromonas

PT140-1 160 Unc. bacterium JF226494 100 Dietzia sp. Actinobacteria Actinobacteria Actinomycetales Dietziaceae Dietzia

PT141-1 160 Unc. bacterium GQ026602 100 Actinomyces oris Actinobacteria Actinobacteria Actinomycetales Actinomycetaceae Actinomyces

PT143-1 160 Corynebacterium imitans Y09044 98 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT143-2 160 Corynebacterium imitans Y09044 99 Corynebacterium imitans Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT144-1 160 Escherichia coli CP002516 100 Escherichia/Salmonella/Citrobacter Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia/Salmonella/Citrobacter

PT145-1 160 Escherichia coli AJ567540 100 Escherichia/ Shigella Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia/Shigella

Streptococcus bovis / Streptococcus PT149-1 160 Streptococcus equinus AB002481 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus lutetiensis / Streptococcus equinus

PT150-1 160 Staphylococcus sciuri AJ421446 100 Staphylococcus sciuri Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus

PT152-1 160 Kocuria koreensis FJ607312 100 Kocuria koreensis Actinobacteria Actinobacteria Actinomycetales Micrococcaceae Kocuria

PT153-1 160 Helcococcus ovis Y16279 91 Helcococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Helcococcus

PT154-1 160 Acinetobacter baumannii X81660 100 Acinetobacter baumannii Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Acinetobacter

Haemophilus parahaemolyticus PT155-1 160 99 Haemophilus parahaemolyticus Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Haemophilus JF506635

PT156-1 160 Klebsiella pneumoniae X87276 100 Klebsiella pneumoniae / Enterobacter Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Klebsiella

PT157-1 160 Unc. bacterium GQ050470 100 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

PT160-1 160 Unc. bacterium JF193426 100 Prevotella sp. Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella

PT161-1 160 Unc. bacterium GQ059838 100 Turicella sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Turicella

28

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

PT162-1 160 Unc. bacterium HM343633 100 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

PT163-1 160 Brevibacterium oceani AM158906 100 Brevibacterium oceani Actinobacteria Actinobacteria Actinomycetales Brevibacteriaceae Brevibacterium

Candidatus Prevotella conceptionensis PT164-1 160 100 Prevotella conceptionensis Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella DQ139963

PT166-1 160 Janibacter anophelis AY837752 100 Janibacter anophelis Actinobacteria Actinobacteria Actinomycetales Intrasporangiaceae Janibacter

PT168-1 160 Leuconostoc lactis AB596941 100 Leuconostoc lactis / Leuconostoc garlicum Firmicutes Bacilli Lactobacillales Leuconostocaceae Leuconostoc

PT168-2 160 Leuconostoc citreum DQ489736 99 Leuconostoc citreum/Leuconostoc palmae Firmicutes Bacilli Lactobacillales Leuconostocaceae Leuconostoc

PT170-1 160 Lactobacillus fermentum EF535258 100 Lactobacillus fermentum Firmicutes Bacilli Lactobacillales Lactobacillaceae Lactobacillus

PT171-1 160 Unc. bacterium AJ583205 100 Unclassified bacterium Unk.Phylum Unk.Class

Pseudomonas jessenii / Pseudomonas PT172-1 160 Pseudomonas jessenii AF068259 100 Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas reinekei Acinetobacter calcoaceticus / Acinetobacter PT173-1 160 Acinetobacter calcoaceticus AJ888984 100 Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Acinetobacter rhizosphaera

PT174-1 160 Unc. bacterium HM186144 96 Micrococcineae bacterium Actinobacteria Actinobacteria Actinomycetales Mobiluncus

PT175-1 160 Unc. bacterium JF236101 99 Streptococcus sp. Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus

Lactobacillus johnsonii/Lactobacillus PT176-1 160 Lactobacillus johnsonii AY531540 100 Firmicutes Bacilli Lactobacillales Lactobacillaceae Lactobacillus taiwanensis

PT177-1 160 Unc. bacterium HM271154 99 Streptococcus sp. Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus

PT178-1 160 Brevibacterium sp. AY577816 100 Brevibacterium sp. Actinobacteria Actinobacteria Actinomycetales Brevibacteriaceae Brevibacterium

PT179-1 160 Propionibacterium granulosum AJ003057 97 Uncultured Actinomycetales bacterium Actinobacteria Actinobacteria Actinomycetales

PT180-1 160 Actinobacillus arthritidis L06084 98 Actinobacillus sp. Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Actinobacillus

PT181-1 160 Unc. bacterium HM306116 99 Janibacter sp. Actinobacteria Actinobacteria Actinomycetales Intrasporangiaceae Janibacter

PT182-1 160 Unc. bacterium GQ080513 98 Dolosigranulum sp. Firmicutes Bacilli Lactobacillales Carnobacteriaceae Dolosigranulum

PT183-1 160 Weissella confusa M23036 100 Weissella confusa Firmicutes Bacilli Lactobacillales Leuconostocaceae Weissella

Brevibacterium linens / Brevibacterium PT184-1 160 Brevibacterium linens EU660372 100 Actinobacteria Actinobacteria Actinomycetales Brevibacteriaceae Brevibacterium iodinum

PT186-1 160 Brachybacterium sp. AB617574 98 Brachybacterium sp. Actinobacteria Actinobacteria Actinomycetales Dermabacteraceae Brachybacterium

PT188-1 160 Ornithinicoccus sp. AB188219 99 Ornithinicoccus sp. Actinobacteria Actinobacteria Actinomycetales Intrasporangiaceae Ornithinicoccus

PT189-1 160 Unc. bacterium clone JF241112 94 Unclassified bacterium Unk.Phylum Unk.Class

PT190-1 160 Unc. bacterium HM292641 100 Brevibacterium sp. Actinobacteria Actinobacteria Actinomycetales Brevibacteriaceae Brevibacterium

29

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

PT191-1 160 Kurthia gibsonii AB271738 100 Kurthia gibsonii Firmicutes Bacilli Bacillales Planococcaceae Kurthia

PT192-1 160 Streptococcus downei AY188350 99 Streptococcus downei Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus

PT193-1 160 Saccharopolyspora halophila DQ923129 98 Pseudonocardiaceae Actinobacteria Actinobacteria Actinomycetales Pseudonocardiaceae Saccharopolyspora

PT194-1 160 Unc. bacterium JF131185 99 Streptobacillus sp. Fusobacteria Fusobacteria Fusobacteriales Leptotrichiaceae Streptobacillus

PT197-1 160 Enterococcus saccharolyticus AF061004 100 Enterococcus saccharolyticus Firmicutes Bacilli Lactobacillales Enterococcaceae Enterococcus

PT199-1 160 Unc. bacterium HM306359 99 Janibacter sp. Actinobacteria Actinobacteria Actinomycetales Intrasporangiaceae Janibacter

PT200-1 160 Unc. bacterium EU779437 90 Uncultured flavobacteriales sp. Bacteroidetes Flavobacteria Flavobacteriales Flavobacteriaceae Wautersiella

PT201-1 160 Unc. bacterium HM333437 96 Uncultured flavobacteriales sp. Bacteroidetes Flavobacteria Flavobacteriales Flavobacteriaceae Cloacibacterium

PT204-1 160 Wautersiella falsenii AM084341 99 Wautersiella falsenii Bacteroidetes Flavobacteria Flavobacteriales Flavobacteriaceae Wautersiella

PT206-1 160 Unc. bacterium HM247613 99 Streptobacillus sp. Fusobacteria Fusobacteria Fusobacteriales Leptotrichiaceae Streptobacillus

PT209-1 160 Unc. bacterium FM875546 96 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

PT212-1 160 Helicobacter pylori CP002336 96 Helicobacter sp. Proteobacteria Epsilonproteobacteria Campylobacterales Helicobacteraceae Helicobacter

PT214-1 160 Prevotella sp. oral taxon GU409530 99 Prevotella sp. Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella

PT222-1 160 Streptococcus sp. oral clone AF432134 100 Streptococcus sp. Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus

PT226-1 160 Unc. bacterium GQ080513 98 Dolosigranulum sp. Firmicutes Bacilli Lactobacillales Carnobacteriaceae Dolosigranulum

PT228-1 160 Dermacoccus sp. PB08bact48 JN412434 100 Micrococcineae bacterium Actinobacteria Actinobacteria Actinomycetales

PT231-1 160 Kribbia sp. DQ985060 100 Micrococcineae bacterium Actinobacteria Actinobacteria Actinomycetales

Moraxella nonliquefaciens/ Moraxella PT233-1 160 Moraxella lacunata D64049 98 Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Moraxella lacunata Actinomycetospora cinnamomea PT235-1 160 100 Actinomycetospora cinnamomea Actinobacteria Actinobacteria Actinomycetales Pseudonocardiaceae Actinomycetospora AB514520

PT238-1 160 Pseudonocardia alni AJ252823 100 Pseudonocardia sp. Actinobacteria Actinobacteria Actinomycetales Pseudonocardiaceae Pseudonocardia

PT239-1 160 Unc. Nesterenkonia sp. HQ674863 100 Uncultured Nesterenkonia sp. Actinobacteria Actinobacteria Actinomycetales Micrococcaceae Nesterenkonia

PT241-1 160 Flexivirga alba AB539735 97 Micrococcineae bacterium Actinobacteria Actinobacteria Actinomycetales

PT246-1 160 Microlunatus soli FJ807672 100 Propionibacteriaceae bacterium Actinobacteria Actinobacteria Actinomycetales Propionibacteriaceae

Bacteroides intestinalis/Bacteroides PT250-1 160 Bacteroides intestinalis AB214328 100 Bacteroidetes Bacteroidia Bacteroidales Bacteroidaceae Bacteroides cellulosilyticus Acinetobacter schindleri/Acinetobacter PT251-1 160 Acinetobacter schindleri FJ860880 100 Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Acinetobacter johnsonii

30

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

Agrostis stolonifera EF115543 / Unc. PT253-1 160 95/97 Cyanobacterium/Chloroplast Cyanobacteria/Chloroplast Chloroplast Bacterium GQ076170 Corynebacterium PT254-1 160 Corynebacterium pilbarense FN295567 100 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium pilbarense/Corynebacterium coyleae

PT261-1 160 Acinetobacter sp. FN395270 100 Acinetobacter sp. Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Acinetobacter

PT278-1 160 Flavobacteriaceae bacterium JN019026 100 Cloacibacterium sp. Bacteroidetes Flavobacteria Flavobacteriales Flavobacteriaceae Cloacibacterium

PT280-1 160 Salinicoccus carnicancri FJ182049 98 Salinicoccus sp. Firmicutes Bacilli Bacillales Staphylococcaceae Salinicoccus

PT281-1 160 Bacteroides uniformis AB050110 100 Bacteroides uniformis Bacteroidetes Bacteroidia Bacteroidales Bacteroidaceae Bacteroides

PT282-1 160 Cystobacter fuscus M94276 100 Cystobacter sp. Proteobacteria Deltaproteobacteria Myxococcales Cystobacteraceae Cystobacter

PT317-1 160 Nicotiana tabacum Z00044 100 Cyanobacterium/Chloroplast Cyanobacteria/Chloroplast Chloroplast

PT360-1 160 Gordonia terrae X79286 100 Gordonia sp. Actinobacteria Actinobacteria Actinomycetales Nocardiaceae Gordonia

PT372-1 160 Actinomyces sp. oral strain AF287751 100 Actinomyces sp. Actinobacteria Actinobacteria Actinomycetales Actinomycetaceae Actinomyces

PT403-1 160 Corynebacterium sp. AY581888 100 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT438-1 160 Unc. bacterium DQ327445 100 Uncultured Clostridium XI sp. Firmicutes Clostridia Clostridiales Peptostreptococcaceae ClostridiumXI

PT487-1 160 Gemella haemolysans L14326 100 Gemella haemolysans Firmicutes Bacilli Bacillales BacillalesIncertaesedisXI Gemella

PT488-1 160 Gemella sanguinis Y13364 100 Gemella sanguinis/Gemella morbillorum Firmicutes Bacilli Bacillales BacillalesIncertaesedisXI Gemella

PT489-1 160 Streptococcus mutans DQ677759 100 Streptococcus mutans Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus

Bacteroides massiliensis/Bacteroides PT490-1 160 Bacteroides massiliensis AY126616 100 Bacteroidetes Bacteroidia Bacteroidales Bacteroidaceae Bacteroides vugatus

PT491-1 160 Unc. bacterium DQ015145 100 Turicibacter sp. Firmicutes Erysipelotricha Erysipelotrichales Erysipelotrichaceae Turicibacter

PT493-1 160 Enterobacter asburiae HQ242719 100 Enterobacter/Yersinia Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Enterobacter/Yersinia

PT494-1 160 Brochothrix thermosphacta AY543023 100 Brochothrix thermosphacta Firmicutes Bacilli Bacillales Listeriaceae Brochothrix

PT496-1 160 Lactobacillus sakei AM113784 100 Lactobacillus sakei Firmicutes Bacilli Lactobacillales Lactobacillaceae Lactobacillus

PT497-1 160 Unc. bacterium EU467572 91 Uncultured Clostridiales bacterium Firmicutes Clostridia Clostridiales

PT502-1 160 Campylobacter mucosalis DQ174173 94 Campylobacter sp. Proteobacteria Epsilonproteobacteria Campylobacterales Campylobacteraceae Campylobacter

PT503-1 160 Actinobacillus ureae AY362900 100 Actinobacillus sp. Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Actinobacillus

PT505-1 160 Unc. bacterium JF145937 100 Neisseriaceae bacterium Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae

PT506-1 160 Staphylococcus haemolyticus AP006716 100 Staphylococcus haemolyticus Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus

31

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

PT507-1 160 Unc. bacterium HM257861 99 Neisseriaceae bacterium Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae

PT508-1 160 Staphylococcus hominis X66101 100 Staphylococcus hominis Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus

PT509-1 160 Unc. bacterium GQ018981 100 Staphylococcus sp. Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus

PT510-1 160 Moraxella lincolni FR822735 99 Moraxella lincolni Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Moraxella

PT511-1 160 Corynebacterium kroppenstedtii Y10077 100 Corynebacterium kroppenstedtii Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT511-2 160 Unc. bacterium GQ061146 99 Corynebacterium sp. Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

Corynebacterium pseudodiphtheriticum Corynebacterium propinquum PT512-1 160 99 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ439343 /Corynebacterium pseudodiphtheriticum Corynebacterium pseudodiphtheriticum Corynebacterium propinquum PT512-2 160 99 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ439343 /Corynebacterium pseudodiphtheriticum Corynebacterium pseudodiphtheriticum Corynebacterium propinquum PT512-3 160 99 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ439343 /Corynebacterium pseudodiphtheriticum Corynebacterium propinquum PT512-4 160 Unc. bacterium HM302210 100 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium /Corynebacterium pseudodiphtheriticum Corynebacterium pseudodiphtheriticum Corynebacterium propinquum PT512-5 160 100 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium AJ439343 /Corynebacterium pseudodiphtheriticum

PT513-1 160 Unc. bacterium JF179978 100 Corynebacterium lubricantis Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT514-1 160 Unc. bacterium HM342925 100 Uncultured Actinomycetales bacterium Actinobacteria Actinobacteria Actinomycetales UnclassifiedActinomycetales

PT515-1 160 Rhodococcus rhodochrous X79288 100 Rhodococcus sp. Actinobacteria Actinobacteria Actinomycetales Nocardiaceae Rhodococcus

PT516-1 160 Enterobacter aerogenes CP002824 100 Enterobacter Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Enterobacter

PT517-1 160 Unc. bacterium GQ038683 99 Neisseriaceae bacterium Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae

PT518-1 160 Corynebacterium imitans Y09044 100 Corynebacterium imitans Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium

PT519-1 160 Unc. bacterium GQ104724 100 Brachybacterium sp. Actinobacteria Actinobacteria Actinomycetales Dermabacteraceae Brachybacterium

Corynebacterium propinquum PT520-1 160 Corynebacterium propinquum X84438 99 Actinobacteria Actinobacteria Actinomycetales Corynebacteriaceae Corynebacterium /Corynebacterium pseudodiphtheriticum

PT521-1 160 Staphylococcus arlettae AB009933 99 Staphylococcus arlettae Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus

Streptococcus oralis/Streptococcus PT522-1 160 Streptococcus oralis AY281080 100 Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus mitis/Streptococcus pneumoniae

PT523-1 160 Streptococcus sp. oral strain AF385525 100 Streptococcus sp. Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus

PT524-1 160 Streptococcus sp. oral strain AY005040 99 Streptococcus sp. Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus Fusobacterium periodonticum/ PT525-1 160 Fusobacterium periodonticum FJ471667 100 Fusobacterium nucleatum/ Fusobacterium Fusobacteria Fusobacteria Fusobacteriales Fusobacteriaceae Fusobacterium russii Brachybacterium faecium/Brachybacterium PT526-1 160 Brachybacterium faecium X91032 100 Actinobacteria Actinobacteria Actinomycetales Dermabacteraceae Brachybacterium fresconis 32

Phylogenetic affiliation based on 16S rRNA gene sequence

Phylotype Length Closest representative Identity Designation Phylum Class Order Family Genus

PT527-1 160 Brachybacterium sp. AM396915 99 Brachybacterium sp. Actinobacteria Actinobacteria Actinomycetales Dermabacteraceae Brachybacterium

PT528-1 160 Dietzia natronolimnaea FJ468335 100 Dietzia sp. Actinobacteria Actinobacteria Actinomycetales Dietziaceae Dietzia

PT529-1 160 Parabacteroides merdae AB238928 100 Parabacteroides merdae Bacteroidetes Bacteroidia Bacteroidales Porphyromonadaceae Parabacteroides

PT531-1 160 Unc. bacterium GQ081220 97 Anaerococcus sp. Firmicutes Clostridia Clostridiales IncertaeSedisXI Anaerococcus

33

Table S3. Gender and age of westernized volunteers. Information on each of the 92 westernized volunteers of this study.

Sample Gender Age Sample Gender Age Sample Gender Age GER1 F 31y GER59 F 43y GER100 M n.d GER2 F 42y GER60 F 44y GER110 F 52y GER4 M 47y GER61 M 53y GER111 M n.d GER5 F 28y GER63 F n.d GER112 F 46y GER6 F 55y GER65 F 20y GER113 F 29y GER7 M 34y GER66 F 55y GER114 M 53y GER8 F 57y GER67 F n.d GER115 M n.d GER9 M 31y GER69 F 23y GER116 M 19y GER10 F 46y GER70 M 35y GER117 M 38y GER11 F 26y GER71 F n.d GER118 F 35y GER12 F 43y GER72 F 23y GER119 F 30y GER13 M 27y GER73 F 44y GER120 M 33y GER14 F 45y GER74 F 49y GER121 F 30y GER15 F 33y GER75 F 43y GER122 F 31y GER18 F 29y GER76 M 49y GER123 M 55y GER19 F 34y GER77 F 39y GER124 F 38y GER20 F 37y GER79 F 51y GER125 M n.d GER21 F 45y GER80 M 27y GER126 F 45y GER25 F 46y GER82 M 56y GER127 F 50y GER26 M 49y GER84 F n.d GER128 F 43y GER27 F 24y GER85 F n.d GER129 M n.d GER28 F 63y GER87 M 40y GER130 F 30y GER29 F 64y GER88 M 19y GER131 M n.d GER30 F 53y GER89 M 65y GER132 M 26y GER33 F 56y GER90 M 59y GER133 M 26y GER34 M 36y GER91 M 42y GER134 F 34y GER40 F 49y GER93 M 53y GER135 M 35y GER45 F 34y GER96 F 46y GER137 M 29y GER46 F 26y GER97 F n.d GER138 M 31y GER52 F 45y GER98 F n.d GER140 F 59y GER58 M 49y GER99 M n.d

y: age in years; n.d: adult, but exact age was not disclosed.

34 Table S4. Gender and age of non-westernized volunteers. Information on each of the 98 non-westernized volunteers of this study.

Age Age Age Sample Gender Sample Gender Sample Gender (years) (years) (years) GAB1 M 30y GAB36 M 3y GAB69 F 41y GAB2 M 41y GAB37 F 40y GAB70 F 7y GAB3 M 23y GAB38 M 49y GAB71 F 6y GAB4 M 37y GAB39 M 45y GAB72 F 37y GAB5 M 44y GAB40 M 84y GAB73 M 35y GAB6 M 14y GAB41 M 45y GAB76 M 40y GAB7 M 47y GAB42 M 25y GAB81 F 5y GAB8 F 3y GAB43 M 23y GAB82 F 7y GAB9 F 4y GAB44 M 29y GAB83 F 9y GAB10 F 3y GAB45 M 74y GAB84 M 10y GAB11 F 3y GAB46 F 49y GAB85 M 3y GAB12 F 2y GAB47 M 50y GAB86 M 13y GAB13 M 7y GAB48 M 19y GAB87 M 43y GAB14 F 30y GAB49 F 74y GAB88 M 10y GAB15 F 24y GAB50 F 77y GAB90 M 6y GAB16 F 25y GAB51 F 74y GAB91 F 5y GAB17 M 43y GAB52 F 24y GAB92 F 4y GAB18 F 21y GAB53 M 45y GAB93 F 11y GAB19 M 61y GAB54 M 23y GAB94 M 10y GAB20 F 41y GAB55 M 24y GAB95 F 9m GAB21 F 70y GAB56 M 37y GAB96 M 3y GAB23 M 39y GAB57 M 10m GAB97 F 3m GAB24 M 1m GAB58 F 4y GAB98 F 22y GAB25 M 6y GAB59 F 3y GAB99 F 18y GAB26 F 4y GAB60 M 44y GAB100 F 2m GAB27 F 5y GAB61 F 47y GAB101 F 19y GAB28 F 13y GAB62 M 43y GAB102 M 2y GAB29 F 4y GAB63 F 20y GAB103 F 19y GAB30 M 2y GAB64 M 28y GAB104 F 19y GAB31 M 1m GAB65 M 27y GAB105 M 3y GAB32 M 12y GAB66 M 42y GAB106 F 3m GAB34 M 42y GAB67 M 49y GAB107 F 4m GAB35 M 18y GAB68 F 9y

y: age in years; m: age in months

35 Table S5. Primers used in this study.

Primer name Primer Sequence (5'-3') Reference

Illumina primers

IlluFBC1 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAAGCCTCAAGAGTTTGATCMTGGCTCAG

lluFBC2 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAAGTTACAAGAGTTTGATCMTGGCTCAG

IlluFBC3 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAATACGCAAGAGTTTGATCMTGGCTCAG

IlluFBC4 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAATCGACAAGAGTTTGATCMTGGCTCAG

IlluFBC5 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAATGATCAAGAGTTTGATCMTGGCTCAG

IlluFBC6 ACACTCTTTCCCTACACGACGCTCTTCCGATCTACCAATCAAGAGTTTGATCMTGGCTCAG

IlluFBC7 ACACTCTTTCCCTACACGACGCTCTTCCGATCTACCGTCCAAGAGTTTGATCMTGGCTCAG

IlluFBC8 ACACTCTTTCCCTACACGACGCTCTTCCGATCTACCTCGCAAGAGTTTGATCMTGGCTCAG

IlluFBC9 ACACTCTTTCCCTACACGACGCTCTTCCGATCTACGAGGCAAGAGTTTGATCMTGGCTCAG

IlluFBC10 ACACTCTTTCCCTACACGACGCTCTTCCGATCTACTATACAAGAGTTTGATCMTGGCTCAG

IlluFBC11 ACACTCTTTCCCTACACGACGCTCTTCCGATCTACTTACCAAGAGTTTGATCMTGGCTCAG

IlluFBC12 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGAAGCCAAGAGTTTGATCMTGGCTCAG

IlluFBC13 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGACCGCAAGAGTTTGATCMTGGCTCAG

IlluFBC14 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGAGTACAAGAGTTTGATCMTGGCTCAG

IlluFBC15 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATATCAAGAGTTTGATCMTGGCTCAG

IlluFBC16 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCATGCAAGAGTTTGATCMTGGCTCAG

IlluFBC17 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCCGTCAAGAGTTTGATCMTGGCTCAG

IlluFBC18 ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGGCTCCAAGAGTTTGATCMTGGCTCAG This study

IlluFBC19 ACACTCTTTCCCTACACGACGCTCTTCCGATCTATAGACCAAGAGTTTGATCMTGGCTCAG

IlluFBC20 ACACTCTTTCCCTACACGACGCTCTTCCGATCTATATTGCAAGAGTTTGATCMTGGCTCAG

IlluFBC21 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCAGATGCAAGAGTTTGATCMTGGCTCAG

IlluFBC22 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCAGCAACAAGAGTTTGATCMTGGCTCAG

IlluFBC23 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCATAGCCAAGAGTTTGATCMTGGCTCAG

IlluFBC24 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCCAGGTCAAGAGTTTGATCMTGGCTCAG

IlluFBC25 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCCGCTTCAAGAGTTTGATCMTGGCTCAG

IlluFBC26 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCCGTAGCAAGAGTTTGATCMTGGCTCAG

IlluFBC27 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCCTGAACAAGAGTTTGATCMTGGCTCAG

IlluFBC28 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCGAATTCAAGAGTTTGATCMTGGCTCAG

IlluFBC29 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCGATCACAAGAGTTTGATCMTGGCTCAG

IlluFBC30 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCGCAACCAAGAGTTTGATCMTGGCTCAG

IlluFBC31 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCGCGCTCAAGAGTTTGATCMTGGCTCAG

IlluFBC32 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCGTCAGCAAGAGTTTGATCMTGGCTCAG

IlluFBC33 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTAAGACAAGAGTTTGATCMTGGCTCAG

IlluFBC34 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTCCATCAAGAGTTTGATCMTGGCTCAG

IlluFBC35 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTCGGCCAAGAGTTTGATCMTGGCTCAG

36 Primer name Primer Sequence (5'-3') Reference

IlluFBC36 ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGCGGCAAGAGTTTGATCMTGGCTCAG

IlluFBC37 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGACCTTCAAGAGTTTGATCMTGGCTCAG

IlluFBC38 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGACGACCAAGAGTTTGATCMTGGCTCAG

IlluFBC39 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGATTAGCAAGAGTTTGATCMTGGCTCAG

IlluFBC40 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGCATCCCAAGAGTTTGATCMTGGCTCAG

IlluFBC41 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGCCAGCCAAGAGTTTGATCMTGGCTCAG

IlluFBC42 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGCGACTCAAGAGTTTGATCMTGGCTCAG

IlluFBC43 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGCTCATCAAGAGTTTGATCMTGGCTCAG

IlluFBC44 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGGCCAACAAGAGTTTGATCMTGGCTCAG

IlluFBC45 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGGCTTCCAAGAGTTTGATCMTGGCTCAG

IlluFBC46 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGGTACCCAAGAGTTTGATCMTGGCTCAG

IlluFBC47 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTAATCCAAGAGTTTGATCMTGGCTCAG

IlluFBC48 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTACGTCAAGAGTTTGATCMTGGCTCAG

IlluFBC49 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCAAGCAAGAGTTTGATCMTGGCTCAG

IlluFBC50 ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCTCTCAAGAGTTTGATCMTGGCTCAG

IlluFBC51 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTACGGTCAAGAGTTTGATCMTGGCTCAG

IlluFBC52 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTACTTGCAAGAGTTTGATCMTGGCTCAG

IlluFBC53 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTATCTCCAAGAGTTTGATCMTGGCTCAG

IlluFBC54 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTCATAACAAGAGTTTGATCMTGGCTCAG

IlluFBC55 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTCCGAGCAAGAGTTTGATCMTGGCTCAG

IlluFBC56 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTCGCCGCAAGAGTTTGATCMTGGCTCAG

IlluFBC57 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTCTAGTCAAGAGTTTGATCMTGGCTCAG

IlluFBC58 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTCTGCCCAAGAGTTTGATCMTGGCTCAG

IlluFBC59 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTGGTCCCAAGAGTTTGATCMTGGCTCAG

IlluFBC60 ACACTCTTTCCCTACACGACGCTCTTCCGATCTTTATGCCAAGAGTTTGATCMTGGCTCAG

Multiplexing AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT PCR

IlluRevAdap GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTTGCTGCCTCCCGTAGGAGT

Index 1 CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTC

Index 2 CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTC

Index 4 CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTC Illumina Inc.

Index 6 CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTC

Index 8 CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTC

37