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888.267.4436 [email protected] www.origene.com Name:CLDN6 mouse monoclonal antibody, clone OTI3E3 (formerly 3E3) Catalog: TA507011 Product Data Sheet - TRUEMAB

Components: • CLDN6 mouse monoclonal antibody, clone OTI3E3 (formerly 3E3) (TA507011) • WB positive control also available: 20ug myc-DDK tagged CLDN6 HEK293T over-expression lysate lyophilized in RIPA buffer (LC412034). (Reconstitute into 20ul of 1x SDS sample buffer before loading; load 5ul per lane as WB control or as desired)

Amount: 100ul

Immunogen: Full length human recombinant protein of human CLDN6(NP_067018) produced in HEK293T cell.

Host: Mouse

Isotype: IgG1

Species Reactivity: Human

Guaranteed WB, IF Applications:

Suggested WB 1:1000, IF 1:100, Dilutions:

Concentration: 1 mg/ml

Buffer: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Purification: Purified from mouse ascites fluids by affinity chromatography

Storage Condition: Shipped at -20C or with ice packs. Upon delivery store at -20C. Dilute in PBS (pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.

Target

Target Name: Homo sapiens claudin 6 (CLDN6)

Alternative Name: OTTHUMP00000159248; claudin 6

Database Link: NP_067018 Entrez Gene 9074 Human

Function: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This gene encodes a component of tight junction strands, which

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. © 2015 OriGene Technologies, Inc. 9620 Medical Center Dr., Suite 200, Rockville, MD 20850 is a member of the claudin family. The protein is an integral membrane protein and is one of the entry cofactors for hepatitis C virus. The gene methylation may be involved in esophageal tumorigenesis. This gene is adjacent to another family member CLDN9 on chromosome 16.[provided by RefSeq, Aug 2010].

HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY CLDN6 (RC200744, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- CLDN6.

Anti-CLDN6 mouse monoclonal antibody (TA507011) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CLDN6(RC200744).

* More validation images may be available on our website: http://www.origene.com/antibody/TA507011.aspx