BIODIVERSITAS ISSN: 1412-033X Volume 20, Number 11, November 2019 E-ISSN: 2085-4722 Pages: 3170-3179 DOI: 10.13057/biodiv/d201109 Chemotypes and antibacterial activity of Matricaria pubescens of Algeria NOUR ELHOUDA MEKHADMI1,2, MESSAOUD RAMDANI1,, TAKIA LOGRADA1, PIERRE CHALARD3, GILLES FIGUEREDO4 1Laboratory of Natural Resource Valorisation, SNV Faculty, Setif 1 University, 19000 Setif, Algeria. Tel. +213 658 101010, email:
[email protected]. 2Department of Biology, Faculty of Natural Science and Life, University of El Oued, BP 789 El-Oued (39000) Algeria. 3Université Clermont Auvergne, CNRS, SIGMA Clermont, ICCF, F-63000 Clermont-Ferrand, France. 4 LEXVA Analytique, 460 rue du Montant, 63110 Beaumont, France. Manuscript received: 27 September 2019. Revision accepted: 10 October 2019. Abstract. Mekhadmi NE, Ramdani M, Lograda T, Chalard P, Figueredo G. 2019. Chemotypes and antibacterial activity of Matricaria pubescens of Algeria. Biodiversitas 20: xxxx. The objectives of this study were to determine the chemical composition and to evaluate the antibacterial activity of Matricaria pubescens L. essential oils from 14 sampling locations in southern Algeria. The extraction of essential oils was carried out by the hydro-distillation; the analysis of chemical composition of essential oil was carried out by GC-MS. Antibacterial activity was performed by disc diffusion method at the undiluted and diluted essential oil (1/1, 1:2, 1:4 and 1:8 v:v of DMSO) against eight species of bacteria (Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Listeria innocua, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enterica and Staphylococcus aureus). The results showed that the average yields of essential oils were 0.4 ± 0.27%.