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Preliminary Program Technical Program The Executive Committee reserves the right to amend the program if necessary. Monday, 24 June 07:30 Continental Breakfast in Exhibit Hall 08:30 - 09:10 Welcome Address General Chair: Christofer Hierold, ETH Zürich, SWITZERLAND Technical Program Introduction Program Chair: Jürgen Brugger, École Polytechnique Fédérale de Lausanne (EPFL), SWITZERLAND 09:00 - 09:45 Plenary Presentation I M1A.P01 HUMAN ORGANS-ON-CHIPS Donald Ingber Harvard University, USA, Harvard Medical School, USA, and Boston Children’s Hospital, USA I will discuss our work on the development of human Organ-on-a-Chip (Organ Chip) microfluidic culture devices lined by living human cells to replace animal testing, accelerate drug development, discover new biomarkers, and advance personalized medicine. Our Organ Chips are effectively living 3D cross sections of major functional units of living organs that contain human organ-specific epithelial cells interfaced with human microvascular endothelial cells that are exposed to fluid flow to mimic vascular perfusion. We also recreate the relevant physicochemical microenvironment of each organ, for example, by recreating breathing motions and an air-liquid interface in lung and trickling flow and peristalsis-like deformations in intestine. We have engineered multiple human Organ Chips, including lung (alveolus and airway), intestine (duodenum, ileum, colon), kidney (proximal tubule and glomerulus), bone marrow, liver, and blood-brain barrier (BBB) chips, as well as fluidically linked BBB Chips and brain neuronal network chips. This Organ Chips have been used to develop various human disease models and uncover new drug targets and potential clinical biomarkers, as well as discover new therapeutics. In addition, multiple different human Organ Chips have been fluidically linked to create an automated ‘human Body-on-Chips’ for real-time analysis of cellular responses to pharmaceuticals, chemicals, and toxins, as well as for quantitative in vitro-to-in vivo translation (IVIVT) of human drug pharmacokinetics in vitro. 09:45 - 10:00 2019 IEEE Daniel E. Noble Award for Emerging Technologies Receipient Thomas W. Kenny Stanford University, USA 10:00 - 10:15 Transducers Early Career Award Presentation 10:15 - 11:00 Break and Exhibit Inspection Session M2A - Bio Microfluidics 11:00 - 11:15 M2A.001 DIGITAL DNA SEQUENCE PROFILING OF RARE EPIGENETIC CANCER BIOMARKERS IN A HIGHLY PARALLELIZED MICROFLUIDIC PLATFORM Christine M. O'Keefe, Sixuan Li, and Tza-Huei Wang Johns Hopkins University, USA We design and construct a digital melt platform to trap DNA molecules at high efficiency in a microfluidic device and assess molecule-by-molecule methylation heterogeneity. We describe the microfluidic design and instrumentation for highly parallelized and efficient single molecule methylation profiling. 11:15 - 11:30 M2A.002 QUAD LIPID BILAYER MODULE WITH 1-GΩ SERIES RESISTORS TOWARD QUANTITATIVE STOCHASTIC-BIOSENSORS Yoshihisa Ito1, Toshihisa Osaki2, Koki Kamiya2, Tetsuya Yamada2, Norihisa Miki1, and Shoji Takeuchi3 1Keio University, JAPAN, 2Kanagawa Institute of Industrial Science and Technology, JAPAN, and 3University of Tokyo, JAPAN Artificial cell membranes with membrane proteins can form sensitive/selective biosensors. But the sensors suffer lengthy detection time at low concentration of analytes because the sensing mechanism relies on stochastic phenomena. Here, we connected independent membrane sensors in parallel, where the current through the multiple membranes was monitored by a single detector. With this format, the detection time is shortened based on the number of the array and the sensing can be quantitative. 11:30 - 11:45 M2A.003 A SINGLE-CELL IMPEDANCE MICRO-CYTOMETER FEATURING 3D ELECTRO-FLUIDIC STRUCTURES MONOLITHICALLY INTEGRATED WITHIN SILVER PDMS Chengwu Han1, Ziheng Liang2, Duli Yu2, and Xiaoxing Xing2 1China-Japan Friendship Hospital, CHINA and 2Beijing University of Chemical Technology, CHINA This work for the first time demonstrates a single-cell impedance micro-cytometer with 3D differential electrodes and fluidic sidewalls completely made of silver- PDMS composites (AgPDMS). Such monolithically integrated electro-fluidic structure is an outcome of one-step molding from a readily used master, obviating extra sacrificial layer patterning as required in existing device incorporating AgPDMS electrodes. 11:45 - 12:00 M2A.004 A CONSTRICTION CHANNEL BASED MICROFLUIDIC FLOW CYTOMETRY ENABLING HIGH- THROUGHPUT QUANTIFICATION OF MULTIPLE TYPES OF INTRACELLULAR PROTEINS IN SINGLE CELLS Lixing Liu, Beiyuan Fan, Hongyu Yang, Deyong Chen, Hua Wei, Guoqing Zhang, Junbo Wang, and Jian Chen Chinese Academy of Sciences (CAS), CHINA This paper presents a constriction channel based microfluidic flow cytometry enabling high-throughput quantification of multiple types of intracellular proteins from large populations (>10,000 cells for each cell type). This microfluidic flow cytometry can distinguish different cells isolated from multiple tumor types and paired tumor cells isolated from the same tumor type. This technique adds a new quantitative dimension to flow cytometry in single-cell analysis. 12:00 - 12:15 M2A.005 INTEGRATED PARALLEL FLOW CYTOMETRY DEVICE WITH TIME GATED SPADS Daiki Sato1, Takahiro Shindo1, Takeshi Mitsunaka1, Yoshihisa Fujimoto1, Kunihiko Iizuka1, Saori Tago2, Yuki Takayama2, Teruo Fujii2, and Soo Hyeon Kim2,3 1Sharp Corporation, JAPAN, 2University of Tokyo, JAPAN and 3JST, PRESTO, Japan We demonstrated parallelized detection of fluorescent microbeads flowing in multiple microfluidic channels coupled with an array of single photon avalanche diodes (SPADs). A CMOS integrated circuit containing SPAD array was used for time-domain separation of fluorescence from excitation light using fast gating and active quenching circuits to realize parallelized detection of fluorescence without complex optical systems. The system can be used for the development of parallelized flow cytometry. 12:15 - 12:30 M2A.006 MICROFLUIDIC ISOLATION OF APTAMERS FOR GLYCAN TARGETS Timothy Olsen, Kyung-Ae Yang, Xin Meng, Milan N. Stojanovic, and Qiao Lin Columbia University, USA This paper demonstrates a microfluidic approach to isolate aptamers, single-stranded oligonucleotides with affinity and specificity for a target, for glycan targets. The approach is capable of rapidly (<1 day) isolating high affinity (uM KD) aptamers with limited sample consumption (<500 µg of glycan) on a single microfluidic platform without the need for offline processes. The presented approach has potential to generate easily accessible sensors for the emerging glycomics field. Session M2B - Chemical Sensors 11:00 - 11:15 M2B.001 MICROFABRICATED SOLUTION CHAMBER FOR HIGH RESOLUTION DIFFRACTED X-RAY TRACKING METHOD TO OBSERVE ION-CHANNEL GATING MOTION Ikkei Yamauchi1, Tomoki Tabuchi1, Yoshikazu Hirai1, Masayuki Iwamoto2, Toshiyuki Tsuchiya1, Hirofumi Shimizu2, and Osamu Tabata1 1Kyoto University, JAPAN and 2University of Fukui, JAPAN We report a novel solution chamber for high resolutions diffracted X-ray tracking method that enables to capture motions of ion-channel proteins upon gating. In this chamber, an observation window consists of silicon nitride membrane to reduce a background noise due to X-ray scattering. The suitable chamber height for both sufficient chamber volume and low background noise was clarified. Motions of ion channel were clearly captured with our chamber, and gating dynamics was successfully analyzed. 11:15 - 11:30 M2B.002 INTEGRATED MULTI-VAPOR MICRO COLLECTOR-INJECTOR (µCOIN) FOR µGC Changhua Zhan1, Muhammad Akbar1, Robert Hower1, Junqi Wang1, Nicolas Nunovero1, Joseph Potkay1,2, and Edward Zellers1 1University of Michigan, USA and 2Veterans Administration Ann Arbor Healthcare System, USA We present a microscale collector-injector (µCOIN) for integration into field-deployable gas chromatographic microsystems (µGC) for analyzing volatile organic compounds. µCOIN consists of a micro passive preconcentrator (µPP) and a micro progressively heated injector (µPHI). We achieved zero-power (diffusional) µPP sampling rates that remain nearly constant for up to 24 hr (fixed, low conc.). The µPHI can provide injection bands < 250 ms wide at ≤ 3 mL/min for most analytes. 11:30 - 11:45 M2B.003 TARGET RECYCLING SIGNAL AMPLIFICATION BASED MICROARRAY PLATFORM FOR HIGH-EFFICIENCY MERCURY AND LEAD IONS DETECTION Shixing Chen1, Yi Yang1, Zonglin Huang1, Hui Wang1, Xuanlin Yang2, Yuelin Wang1, Shiping Song1 and Tie Li1 1Chinese Academy of Sciences (CAS), CHINA and 2Institute of NBC Defense, PLA Army, CHINA For trace analysis of heavy metals, a Y type DNA structure based signal amplification system and microarray platform is announced to detect lead and mercury. The results show LOD of 0.5 pM and 0.6 pM for Hg2+ and Pb2+; high discriminating ability from interfering ions such as Mn2+, Cu2+ and Ca2+; the optimal incubation time was only 0.5 hour. Compared to the enzyme method, the novel system holds great application ability with robust, ultra-high sensitivity, high selectivity and high-throughput. 11:45 - 12:00 M2B.004 IN-PLANTA NITRATE DETECTION USING INSERTABLE PLANT MICROSENSOR Yueyi Jiao1, Xinran Wang1, Yuncong Chen1, Michael J. Castellano1, James C. Schnable2, Patrick S. Schnable1, and Liang Dong1 1Iowa State University, USA and 2University of Nebraska-Lincoln, USA We develop a sensor that measures plant nitrate levels over time. 12:00 - 12:15 M2B.005 REAL-TIME EVALUATION OF SCATTERING STRENGTH ON GRAPHENE FET FOR SELECTIVE SENSING OF
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