http://www.chemistryexplained.com/images/chfa_04_img0954.jpg http://www.bio-world.com/images/reagents.jpg http://www.nlm.nih.gov/visibleproofs/media/detailed/iii_d_142b.jpg Radioimmunoassay 2310302 RAD TRACER Lerson Tanasugarn, Ph.D. Department of Biochemistry, Faculty of Science, Chulalongkorn University Klum 515, Phone 662-218-5424, Departmental FAX 662-218-5418 Email:
[email protected] Handout:
[email protected]/public/biochemistry/2010 2310302 RIA.pdf Copyright © 2010 by Lerson Tanasugarn. All rights reserved. 1 Contents • History of radioimmunoassay • Principles of radioimmunoassay • Non-isotope immunoassay • Methods • Applications Copyright © 2010 by Lerson Tanasugarn. All rights reserved. 2 History http://2.bp.blogspot.com/ http://www.aps.org/publications/ Rall, J. E. 1990. Solomon A. http://www.invention-ifia.ch/ _DZH2cmCoois/SLFpb8J_GPI/ apsnews/199810/images/ Berson 1918-1972. National images/Rosalyn_Yalow2.jpg AAAAAAAAFvc/4fpGNpLZ6YE/s400/ rosalyn_sussman_yalow.jpg Academy of Sciences. Nobel_Laureate_1977_Rosalyn_Yalow .bmp Rosalyn Sussman Yalow 1921- Solomon Berson 1918 - 1972 • City College of NY - BS and MSc • Born in NYC (NY+German) • Anatomy instructorship NYU • Hunter College BS • MD (NYU 1945) • PhD in physics (U. Illinois 1945) • Intern (Boston City Hospital 1945-46) • Joined the Bronx V.A. Hospital to set up • US Army (1946-48) its radioisotope service • Recruited by Yalow to work in the Radioisotope • Recruit Berson to form a research team Service of the Bronx V.A. Hospital (to support her work) • Chief of Radioisotope Service (forerunner of the Nuclear Medicine Service) 1954 • Rall, J. E. 1990. Solomon A. Berson 1918-1972 A Biographical Memoir. National Academy of Sciences Washington, D.C. http://books.nap.edu/html/biomems/sberson.pdf • Yalow, R.