FCN1 293 Cell Lysate Storage: Store at -80°C

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From Biology to Discovery™ FCN1 293 Cell Lysate Storage: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored Subcategory: Cell Lysate at -20°C. Product is guaranteed 6 months from the date of Cat. No.: 400709 shipment. Unit: 0.1 mg For research use only, not for diagnostic or therapeutic Description: procedures. Antigen standard for ficolin (collagen/fibrinogen domain containing) 1 (FCN1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. Applications: ELISA, WB, IP Format: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). Alternate Names: ficolin (collagen/fibrinogen domain- containing) 1; M-ficolin; ficolin-A; ficolin-alpha; collagen/fibrinogen domain-containing protein 1; ficolin-1; FCN1; FCNM Accession No.: NP_001994 Application Notes: WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. Abbiotec, LLC • 7985 Dunbrook Rd, Ste A, San Diego, CA 92126 • USA Ph: 858.586.0500 • Fax: 858.586.6252 • Email: [email protected] • www.abbiotec.com.

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Variation in FCN1 Affects Biosynthesis of Ficolin-1 and Is Associated With
Genes and Immunity (2012) 13, 515–522 & 2012 Macmillan Publishers Limited All rights reserved 1466-4879/12 www.nature.com/gene ORIGINAL ARTICLE Variation in FCN1 affects biosynthesis of ficolin-1 and is associated with outcome of systemic inflammation L Munthe-Fog1, T Hummelshoj1, C Honore´ 1, ME Moller1, MO Skjoedt1, I Palsgaard1, N Borregaard2, HO Madsen1 and P Garred1 Ficolin-1 is a recognition molecule of the lectin complement pathway. The ficolin-1 gene FCN1 is polymorphic, but the functional and clinical consequences are unknown.The concentration of ficolin-1 in plasma and FCN1 polymorphisms in positions À 1981 (rs2989727), À 791 (rs28909068), À 542 (rs10120023), À 271 (rs28909976), À 144 (rs10117466) and þ 7918 (rs1071583) were determined in 100 healthy individuals. FCN1 expression by isolated monocytes and granulocytes and ficolin-1 levels in monocyte culture supernatants were assessed in 21 FCN1-genotyped individuals. FCN1 polymorphisms were determined in a cohort of 251 patients with systemic inflammation. High ficolin-1 plasma levels were significantly associated with the minor alleles in position À 542 and À 144. These alleles were also significantly associated with high FCN1 mRNA expression. The level of ficolin-1 in culture supernatants was significantly higher in individuals homozygous for the minor alleles at positions À 542 and À 144. Homozygosity for these alleles was significantly associated with fatal outcome in patients with systemic inflammation. None of the other investigated polymorphisms were associated with FCN1 and ficolin-1 expression, concentration or disease outcome. Functional polymorphic sites in the promoter region of FCN1 regulate both the expression and synthesis of ficolin-1 and are associated with outcome in severe inflammation.
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Human Lectins, Their Carbohydrate Affinities and Where to Find Them
biomolecules Review Human Lectins, Their Carbohydrate Affinities and Where to Review HumanFind Them Lectins, Their Carbohydrate Afﬁnities and Where to FindCláudia ThemD. Raposo 1,*, André B. Canelas 2 and M. Teresa Barros 1 1, 2 1 Cláudia D. Raposo * , Andr1 é LAQVB. Canelas‐Requimte,and Department M. Teresa of Chemistry, Barros NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829‐516 Caparica, Portugal; [email protected] 12 GlanbiaLAQV-Requimte,‐AgriChemWhey, Department Lisheen of Chemistry, Mine, Killoran, NOVA Moyne, School E41 of ScienceR622 Co. and Tipperary, Technology, Ireland; canelas‐ [email protected] NOVA de Lisboa, 2829-516 Caparica, Portugal; [email protected] 2* Correspondence:Glanbia-AgriChemWhey, [email protected]; Lisheen Mine, Tel.: Killoran, +351‐212948550 Moyne, E41 R622 Tipperary, Ireland; [email protected] * Correspondence: [email protected]; Tel.: +351-212948550 Abstract: Lectins are a class of proteins responsible for several biological roles such as cell‐cell in‐ Abstract:teractions,Lectins signaling are pathways, a class of and proteins several responsible innate immune for several responses biological against roles pathogens. such as Since cell-cell lec‐ interactions,tins are able signalingto bind to pathways, carbohydrates, and several they can innate be a immuneviable target responses for targeted against drug pathogens. delivery Since sys‐ lectinstems. In are fact, able several to bind lectins to carbohydrates, were approved they by canFood be and a viable Drug targetAdministration for targeted for drugthat purpose. delivery systems.Information In fact, about several specific lectins carbohydrate were approved recognition by Food by andlectin Drug receptors Administration was gathered for that herein, purpose. plus Informationthe specific organs about speciﬁc where those carbohydrate lectins can recognition be found by within lectin the receptors human was body.
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Adiponectin and Related C1q/TNF-Related Proteins Bind Selectively to Anionic Phospholipids and Sphingolipids
Adiponectin and related C1q/TNF-related proteins bind selectively to anionic phospholipids and sphingolipids Jessica J. Yea,b, Xin Bianc,d, Jaechul Lima,b, and Ruslan Medzhitova,b,1 aHHMI, Yale University, New Haven, CT 06520; bDepartment of Immunobiology, Yale University School of Medicine, New Haven, CT 06520; cDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT 06520; and dDepartment of Neuroscience, Yale University School of Medicine, New Haven, CT 06520 Contributed by Ruslan Medzhitov, April 14, 2020 (sent for review December 20, 2019; reviewed by Ido Amit and G. William Wong) Adiponectin (Acrp30) is an adipokine associated with protection 5-mers of trimers, respectively referred to as low molecular weight from cardiovascular disease, insulin resistance, and inflammation. (LMW), medium molecular weight (MMW), and high molecular Although its effects are conventionally attributed to binding weight (HMW) complexes. Adiponectin can also be cleaved by Adipor1/2 and T-cadherin, its abundance in circulation, role in serum elastases and thrombin between the globular C1q-like head ceramide metabolism, and homology to C1q suggest an overlooked domain and the collagenous tail domain, forming globular adi- role as a lipid-binding protein, possibly generalizable to other C1q/ ponectin (gAd). While gAd appears to bind AdipoR1/2 and ac- TNF-related proteins (CTRPs) and C1q family members. To investi- tivate AMPK more strongly than full-length protein (19, 20), levels gate this, adiponectin, representative family members, and variants of HMW multimers are more strongly associated with insulin were expressed in Expi293 cells and tested for binding to lipids in sensitivity (21, 22), have a longer half-life in circulation (18), and liposomes using density centrifugation.
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Microarray Analysis of Novel Genes Involved in HSV- 2 Infection
Microarray analysis of novel genes involved in HSV- 2 infection Hao Zhang Nanjing University of Chinese Medicine Tao Liu ( [email protected] ) Nanjing University of Chinese Medicine https://orcid.org/0000-0002-7654-2995 Research Article Keywords: HSV-2 infection,Microarray analysis,Histospecic gene expression Posted Date: May 12th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-517057/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/19 Abstract Background: Herpes simplex virus type 2 infects the body and becomes an incurable and recurring disease. The pathogenesis of HSV-2 infection is not completely clear. Methods: We analyze the GSE18527 dataset in the GEO database in this paper to obtain distinctively displayed genes(DDGs)in the total sequential RNA of the biopsies of normal and lesioned skin groups, healed skin and lesioned skin groups of genital herpes patients, respectively.The related data of 3 cases of normal skin group, 4 cases of lesioned group and 6 cases of healed group were analyzed.The histospecic gene analysis , functional enrichment and protein interaction network analysis of the differential genes were also performed, and the critical components were selected. Results: 40 up-regulated genes and 43 down-regulated genes were isolated by differential performance assay. Histospecic gene analysis of DDGs suggested that the most abundant system for gene expression was the skin, immune system and the nervous system.Through the construction of core gene combinations, protein interaction network analysis and selection of histospecic distribution genes, 17 associated genes were selected CXCL10,MX1,ISG15,IFIT1,IFIT3,IFIT2,OASL,ISG20,RSAD2,GBP1,IFI44L,DDX58,USP18,CXCL11,GBP5,GBP4 and CXCL9.The above genes are mainly located in the skin, immune system, nervous system and reproductive system.
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Effects of Sex and Aging on the Immune Cell Landscape As Assessed by Single-Cell Transcriptomic Analysis
Effects of sex and aging on the immune cell landscape as assessed by single-cell transcriptomic analysis Zhaohao Huanga,1, Binyao Chena,1, Xiuxing Liua,1,HeLia,1, Lihui Xiea,1, Yuehan Gaoa,1, Runping Duana, Zhaohuai Lia, Jian Zhangb, Yingfeng Zhenga,2, and Wenru Sua,2 aState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, China; and bDepartment of Clinical Research Center, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, China Edited by Lawrence Steinman, Stanford University School of Medicine, Stanford, CA, and approved June 22, 2021 (received for review November 19, 2020) Sex and aging influence the human immune system, resulting in the immune system integrates numerous interconnected compo- disparate responses to infection, autoimmunity, and cancer. How- nents, pathways, and cell types in sex and aging. ever, the impact of sex and aging on the immune system is not yet To this end, we profiled the transcriptomes of peripheral im- fully elucidated. Using small conditional RNA sequencing, we mune cells sampled from men and women of two distinct age found that females had a lower percentage of natural killer (NK) groups. Then, we investigated sex- and age-related differences in cells and a higher percentage of plasma cells in peripheral blood PBMC immune cell composition, molecular programs, and com- compared with males. Bioinformatics revealed that young females munication network. Extensive differences in all of these aspects exhibited an overrepresentation of pathways that relate to T and were observed between sexes, particularly in aging. Compared – B cell activation. Moreover, cell cell communication analysis revealed with males, females exhibited a higher expression of T cell (TC)– evidence of increased activity of the BAFF/APRIL systems in females.
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Integrated bioinformatics approach to understand immune-related key genes and pathways in chronic spontaneous urticaria wenxing su Second Aliated Hospital of Soochow University biao huang First Aliated Hospital of Soochow University ying zhao Second Aliated Hospital of Soochow University xiaoyan zhang Second Aliated Hospital of Soochow University lu chen second aliated hospital of soochow university jiang ji ( [email protected] ) Second Aliated Hospital of Soochow University qingqing Jiao rst aliated hospital of soochow university Research Keywords: Chronic spontaneous urticaria, bioinformatical analysis, differentially expressed genes, immune inltration Posted Date: December 31st, 2020 DOI: https://doi.org/10.21203/rs.3.rs-137346/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/29 Abstract Background Chronic spontaneous urticaria (CSU) refers to recurrent urticaria that lasts for more than 6 weeks in the absence of an identiable trigger. Due to its recurrent wheal and severe itching, CSU seriously affects patients' life quality. There is currently no radical cure for it and its vague pathogenesis limits the development of targeted therapy. With the goal of revealing the underlying mechanism, two data sets with accession numbers GSE57178 and GSE72540 were downloaded from the Gene Expression Omnibus (GEO) database. After identifying the differentially expressed genes (DEGs) of CSU skin lesion samples and healthy controls, four kinds of analyses were performed, namely functional annotation, protein- protein interaction (PPI) network and module construction, co-expression and drug-gene interaction prediction analysis, and immune and stromal cells deconvolution analyses. Results 92 up-regulated genes and 7 down-regulated genes were selected for subsequent analyses.
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A Genome-Wide Association Study of Basal Transepidermal Water Loss Finds That Variants at 9Q34.3 Are Associated with Skin Barrier Function
LETTER TO THE EDITOR A Genome-Wide Association Study of Basal Transepidermal Water Loss Finds that Variants at 9q34.3 Are Associated with Skin Barrier Function Journal of Investigative Dermatology (2017) -, -e-; doi:10.1016/j.jid.2016.11.030 TO THE EDITOR Jiangsu Province, aged between 31 and After quality-control filters, the GWAS Epidermal homeostasis and barrier 87 years. This research was conducted was carried out on 795,279 geno- permeability are very important proper- with official approval from the ethics typed single-nucleotide polymorphisms ties of human skin. Transepidermal wa- committee of Fudan University, (SNPs) and 7,203,134 imputed SNPs ter loss (TEWL), the passive diffusion of Shanghai, China. All participants pro- (see Supplementary Materials for the water from the hydrated layers of the vided written informed consent. TEWL details). We found a variant on chro- dermis and epidermis toward those measurement was carried out with mosome band 9q34.3 to be significantly layers with a lower water content a DermaMeter Professional 100 associated with TEWL (rs10858314, b ¼ e (Nilsson, 1977), has been widely used to (VASEMA GmbH, Vienna, Austria) on e0.211 Æ 0.038, P ¼ 3.11 Â 10 8; determine epidermal permeability bar- the right cheek (see Supplementary see Supplementary Figure S3 online). rier status (Fluhr et al., 2006). For Materials and Supplementary Table S1 To validate our finding, we performed example, TEWL measurement helped to online for details). Because the a second GWAS using the same establish that skin barrier function is obtained TEWL values did not follow phenotyping protocol on a replication compromised in skin diseases such as the normal distribution (Shapiro-Wilk set including 366 healthy Han e atopic dermatitis (AD) (Elias, 2008).
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Gene Expression Profiling of the Extracellular Matrix Signature In
International Journal of Molecular Sciences Article Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing Julia Etich 1,* , Manuel Koch 2,3, Raimund Wagener 2,4, Frank Zaucke 1 , Mario Fabri 5 and Bent Brachvogel 2,6,7 1 Dr. Rolf M. Schwiete Research Unit for Osteoarthritis, Orthopaedic University Hospital Friedrichsheim gGmbH, 60528 Frankfurt/Main, Germany; [email protected] 2 Center for Biochemistry, Medical Faculty, University of Cologne, 50931 Cologne, Germany; [email protected] (M.K.); [email protected] (R.W.); [email protected] (B.B.) 3 Institute for Dental Research and Oral Musculoskeletal Biology, Medical Faculty, University of Cologne, 50931 Cologne, Germany 4 Center for Molecular Medicine, University of Cologne, 50931 Cologne, Germany 5 Department of Dermatology, University of Cologne, 50931 Cologne, Germany; [email protected] 6 Department of Pediatrics and Adolescent Medicine, Experimental Neonatology, Medical Faculty, University of Cologne, 50931 Cologne, Germany 7 Cologne Center for Musculoskeletal Biomechanics, University of Cologne, 50931 Cologne, Germany * Correspondence: [email protected] Received: 23 September 2019; Accepted: 11 October 2019; Published: 14 October 2019 Abstract: The extracellular matrix (ECM) provides structural support for tissue architecture and is a major effector of cell behavior during skin repair and inflammation. Macrophages are involved in all stages of skin repair but only limited knowledge exists about macrophage-specific expression and regulation of ECM components. In this study, we used transcriptome profiling and bioinformatic analysis to define the unique expression of ECM-associated genes in cultured macrophages.
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FCN1 Antibody (C-Term) Blocking Peptide Synthetic Peptide Catalog # Bp17029b
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 FCN1 Antibody (C-term) Blocking Peptide Synthetic peptide Catalog # BP17029b Specification FCN1 Antibody (C-term) Blocking Peptide - FCN1 Antibody (C-term) Blocking Peptide - Background Product Information The ficolin family of proteins are characterized Primary Accession O00602 by thepresence of a leader peptide, a short N-terminal segment, followedby a collagen-like region, and a C-terminal fibrinogen-like FCN1 Antibody (C-term) Blocking Peptide - Additional Information domain.The collagen-like and the fibrinogen-like domains are also foundseparately in other proteins such as Gene ID 2219 complement protein C1q, C-typelectins known as collectins, and tenascins. However, all Other Names theseproteins recognize different targets, and Ficolin-1, Collagen/fibrinogen are functionallydistinct. Ficolin 1 encoded by domain-containing protein 1, Ficolin-A, FCN1 is predominantly expressed inthe Ficolin-alpha, M-ficolin, FCN1, FCNM peripheral blood leukocytes, and has been Format postulated tofunction as a plasma protein with Peptides are lyophilized in a solid powder elastin-binding activity. format. Peptides can be reconstituted in solution using the appropriate buffer as FCN1 Antibody (C-term) Blocking Peptide - needed. References Storage Honore, C., et al. J. Leukoc. Biol. Maintain refrigerated at 2-8°C for up to 6 88(1):145-158(2010)Davila, S., et al. Genes months. For long term storage store at Immun. 11(3):232-238(2010)Gout, E., et al. J. -20°C. Biol. Chem. 285(9):6612-6622(2010)Wittenborn, T., et al. J Precautions Innate Immun 2(2):167-180(2010)Tanio, M., et This product is for research use only.
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The Evolution of the Immune System: Conservation and Diversification
Title The Evolution of the Immune System Conservation and Diversification Page left intentionally blank The Evolution of the Immune System Conservation and Diversification Davide Malagoli Department of Life Sciences Biology Building, University of Modena and Reggio Emilia, Modena, Italy AMSTERDAM • BOSTON • HEIDELBERG • LONDON NEW YORK • OXFORD • PARIS • SAN DIEGO SAN FRANCISCO • SINGAPORE • SYDNEY • TOKYO Academic Press is an imprint of Elsevier Academic Press is an imprint of Elsevier 125 London Wall, London EC2Y 5AS, United Kingdom 525 B Street, Suite 1800, San Diego, CA 92101-4495, United States 50 Hampshire Street, 5th Floor, Cambridge, MA 02139, United States The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, UK Copyright © 2016 Elsevier Inc. All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Details on how to seek per- mission, further information about the Publisher’s permissions policies and our arrangements with organizations such as the Copyright Clearance Center and the Copyright Licensing Agency, can be found at our website: www.elsevier.com/permissions. This book and the individual contributions contained in it are protected under copyright by the Publisher (other than as may be noted herein). Notices Knowledge and best practice in this field are constantly changing. As new research and experience broaden our understanding, changes in research methods, professional practices, or medical treatment may become necessary. Practitioners and researchers must always rely on their own experience and knowledge in evaluating and using any information, methods, compounds, or experiments described herein.
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Xu et al. Cell Discovery (2020) 6:73 Cell Discovery https://doi.org/10.1038/s41421-020-00225-2 www.nature.com/celldisc ARTICLE Open Access The differential immune responses to COVID-19 in peripheral and lung revealed by single-cell RNA sequencing Gang Xu1,FurongQi1, Hanjie Li2, Qianting Yang1, Haiyan Wang1,XinWang1, Xiaoju Liu3, Juanjuan Zhao1, Xuejiao Liao1, Yang Liu1,LeiLiu1,ShuyeZhang 4 and Zheng Zhang 1 Abstract Understanding the mechanism that leads to immune dysfunction in severe coronavirus disease 2019 (COVID-19) is crucial for the development of effective treatment. Here, using single-cell RNA sequencing, we characterized the peripheral blood mononuclear cells (PBMCs) from uninfected controls and COVID-19 patients and cells in paired broncho-alveolar lavage fluid (BALF). We found a close association of decreased dendritic cells (DCs) and increased monocytes resembling myeloid-derived suppressor cells (MDSCs), which correlated with lymphopenia and inflammation in the blood of severe COVID-19 patients. Those MDSC-like monocytes were immune-paralyzed. In contrast, monocyte-macrophages in BALFs of COVID-19 patients produced massive amounts of cytokines and chemokines, but secreted little interferons. The frequencies of peripheral T cells and NK cells were significantly decreased in severe COVID-19 patients, especially for innate-like T and various CD8+ T cell subsets, compared to healthy controls. In contrast, the proportions of various activated CD4+ T cell subsets among the T cell compartment, including Th1, Th2, and Th17-like cells were increased and more clonally expanded in severe COVID-19 patients. ’ 1234567890():,; 1234567890():,; 1234567890():,; 1234567890():,; Patients peripheral T cells showed no sign of exhaustion or augmented cell death, whereas T cells in BALFs produced higher levels of IFNG, TNF, CCL4, CCL5, etc.
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