DOCSLIB.ORG

Halophilic Bacteria and Their Biomolecules: Recent Advances and Future Applications in Biomedicine

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Halophilic Bacteria and Their Biomolecules: Recent Advances and Future Applications in Biomedicine Mor. J. Agri. Sci. 1(6): 323-335, December 2020 323 Halophilic bacteria and their biomolecules: Recent advances and future applications in biomedicine Naima BOU MHANDI1 Abstract The organisms thriving under extreme conditions better than any other organism living on Earth fascinate by their hostile growing parameters, physiological features and their 1 Specialized Center of Valoriza- tion and Technology of Sea production of valuable bioactive metabolites. This is the case of halophilic bacteria that Products, National Institute grow optimally at high salinities and are able to produce biomolecules of pharmaceutical of Fisheries Research, Agadir, interest for therapeutic applications. As long as the microbiota is being approached by mas- Morocco sive sequencing, novel insights are revealing the environmental conditions in which the compounds are produced in the microbial community without more stress than sharing the same salt substratum with their peers. In this review are reported the molecules produced * Corresponding author [email protected] by halophilic bacteria with a spectrum of action in vitro: antimicrobial and anticancer. The action mechanisms of these molecules, the urgent need to introduce alternative lead Received 21/12/2020 compounds and the current aspects on the exploitation and its limitations are discussed. Accepted 30/12/2020 Keywords: Halophilic bacteria, biomolecules, biomedicine, antimicrobial INTRODUCTION Ventosa et al., 2004; Amoozegar et al., 2016). Extremely halophilic bacteria generally grow slowly. Halophilic Halophiles are organisms represented by archaea, bacte- bacteria can be identified commonly by phenotypic ria and eukarya for which the main characteristic is their characterization as well as 16S rRNA gene sequences salinity requirement, halophilic “salt-loving”. Halophilic (Fendrihan et al., 2012). Moderately halophilic bacteria microorganisms constitute the natural microbial com- are dominant in mostly hypersaline environments and munities of hypersaline ecosystems, which are widely they constitute a major proportion of total microbial distributed around the world (Oren, 2008). They require population in the hypersaline environments and play a sodium ions for their growth and metabolism. Thus, major ecological role. based on the NaCl optimal requirement for growth, the halophiles are classified in three different categories: Currently, a large number of bacterial species related slight (1–3%); moderate (3–15%) and extreme (15– to different bacterial phyla, especially gram-positive, 30%) (Kushner, 1978; Kushner and Kamekura, 1988). In showed moderately halophilic response. Moderately contrast to halotolerant organisms, obligate halophiles halophilic bacteria include members of Proteobacteria require NaCl concentrations higher than 3% NaCl or (Halomonas, Chromohalobacter, Pseudomonas, Ma- above of seawater, with about 3.5% NaCl (Rodriguez- rinobacter, Rhodospirillum, Aeromonas, Alteromonas, Valera et al, 1981). The tolerance parameters and salt Rhodovibrio, Halovibrio and Alcaligenes), Firmicutes requirements are dependent on temperature, pH and (Halobacillus, Virgibacillus, Oceanobacillus, Staphylo- growth medium. In this way, the halophiles are adapted coccus, Gracilibacillus, Clostridium, Pontibacillus, Spo- and limited by specific environmental factors. Those -mi rosarcina and Planococcus), Actinobacteria (Kocuria, croorganisms able to survive and optimally thrive under Streptomyces and Rubrobacter), Actinomycetes (Nocar- a wide spectrum of extreme environmental factors are dia, Nocardiopsis, Streptomonospora, Actinopolyspora designated as poly-extremophiles (Seckbach et al., 2013; and Nesterenkonia) and Bacteriodetes (Flavobacterium, Bowers et al., 2009). In fact, a halophilic microorganism Salinibacter and Polaribacter). can also be alkaliphile, designated as haloalkaliphile, Mostly, extreme halophilic bacteria contain a variety of growing optimally or very well at pH values above 9.0, carotenoids as carotenoids help membrane stabilization but cannot grow at the near neutral pH value of 6.5 in Thermus thermophiles, Ruberobacter radiotolerans (Mesbah and Wiegel, 2012). and help to tolerate the high osmotic stress in Halobacte- Halophilic bacteria are cocci, rod, triangular and even rim spp. (Amoozegar et al., 2016, Fendrihan et al., 2012, square-shaped. Some strains are pleiomorphic especial- Sasaki et al., 2012) Many gram-positive bacteria isolated ly when the ionic conditions of the media are altered and from different saline environments (salt lakes, salt mines most lie below the NaCl level of 1M/L. The physiology of and salt marshes) also have carotenoids which indicate the moderate and extreme halophilic bacteria is affected the crucial role of carotenoids in osmotolerance of these by change in salt concentration, growth temperature bacteria (Ii et al., 2015). and nature of available nutrients (Fourcans et al., 2006; © Moroccan Journal of Agricultural Sciences • e-ISSN: 2550-553X www.techagro.org 324 Bou Mhandi: Halophilic bacteria and their biomolecules Cyanobacteria are characterized by the presence of chlo- In this review, we focus on the biomolecules described rophyll and phycobilin pigments. They are photosyn- as antimicrobial or anticancer compounds produced thetic bacteria (planktonic biomass) and form microbial by halophilic bacteria and discuss current and future mats in many hypersaline lakes (Baxter et al., 2014). perspectives in this field. Aphanothece halophytica is an extreme halophile form of brown layer of microbial mat on the water surface. Most ANTIMICROBIAL COMPOUNDS cyanobacteria use glycine betaine as the major compat- ible solute which they take from the medium or synthe- The current situation of antibiotic resistance propaga- size from choline (Boornburapong et al., 2016). Many tion poses a global threat to public health. Over the past genera related to halophilic cyanobacteria have been decades, antibiotics have saved millions of lives, but their described from the Dead Sea, the Great Salt Lake, Solar misuse has led to the emergence of multi-drug resistant Lake and other salt lakes and ponds but the cyanobacte- bacteria (MDR), reducing or nullifying their effective- rial diversity has not been studied extensively from the ness. Recently, the continuous increase in antibiotic hypersaline environments (Tripathi et al., 2013). Moder- resistance is reaching critical levels, which implies an ately halophilic purple sulfur bacteria like Chromatium increase in morbidity in the healthy population and an spp. have the ability to store sulfur granules inside cells imminent risk for hospitalized patients (Tseng et al., and they grow phototrophically by using glycerol or gly- 2018; Peters et al., 2019). In fact, the main cause of death colate. Rhodospirilum salexigens (purple non-sulfur bac- of patients are attributable to complications due to MDR terium) can use glycine, betaine or ectoine as osmolytes. infections (Cassini et al., 2019). Preventing the return Sulfur oxidizing bacteria are halophilic gram-positive, to the pre-antibiotic era is one of the main challenges for science. The urgent need to introduce new effective filamentous CO2 fixing bacteria. They oxidize sulfur and hydrogen sulfide to form sulfates. For example, Achro- antimicrobial therapies is leading to the exploitation of matium volutans, a filamentous bacterium was isolated all possible natural and sustainable resources, including from solar lakes and Thiobacillus halophilus a halophilic extreme environments as a promising resource for new chemoauthotrpohic bacterium was isolated from the antibiotic discovery. The first antimicrobial compounds hypersaline lake, Australia (Sorokin et al., 2014). from halophilic microorganisms were reported in 1982 by Rodriguez-Valera et al. (1982). Halocin was the term BIOTECHNOLOGICAL IMPORTANCE OF coined for substances secreted by several members of the genus Halobacterium capable of causing death and lysis HALOPHILIC BACTERIA of the surrounding microbiota. Halocins are the pro- The exploitation of extremophiles is having special teins and antimicrobial peptides (AMPs) produced by importance in the development of new molecules with haloarchaea (Rodriguez-Valera et al., 1982; Gohel et al., potential applications in biomedicine. Current efforts 2015). Despite the ecological and environmental role of are focused primarily to cover the urgent health needs, several halocins, their action against human pathogens especially those that represent the main global threats, has been less studied. In the fight against time, the clini- cancer and antibiotic resistance. The great metabolic cal significance of halophilic microorganisms is minorly versatility of halophilic microorganisms, their low nu- reported and the antimicrobial action against the most tritional requirements and their genetic machineries of important risk group of human pathogens ESKAPE: adaptation to harsh conditions, like nutrient starvation, Enterococcus faecium, Staphylococcus aureus, Klebsiella desiccation, high sun radiation, and high ionic strength, pneumoniae, Acinetobacter baumannii and Pseudomo- make them promising candidates and a hope for drug nas aeruginosa, still remains as a potential. According to discovery (Charlesworth and Burns, 2015). Continuous the data inferred, the antagonistic action identified and advances in “omics” and bioinformatic tools are reveal- the production of bioactive compounds by halophilic ing uncountable encoding genes for the production of microorganisms are derived
Load more

Recommended publications
  • Actinobacteria in Algerian Saharan Soil and Description Sixteen New
    Revue ElWahat pour les Recherches et les Etudes Vol.7n°2 (2014) : 61 – 78 Revue ElWahat pour les recherches et les Etudes : 61 – 78 ISSN : 1112 -7163 Vol.7n°2 (2014) http://elwahat.univ-ghardaia.dz Diversity of Actinobacteria in Algerian Saharan soil and description of sixteen new taxa Bouras Noureddine 1,2 , Meklat Atika 1, Boubetra Dalila 1, Saker Rafika 1, Boudjelal Farida 1, Aouiche Adel 1, Lamari Lynda 1, Zitouni Abdelghani 1, Schumann Peter 3, Spröer Cathrin 3, Klenk Hans -Peter 3 and Sabaou Nasserdine 1 1- Affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria; [email protected] 2- Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, Ghardaïa 47000, Algeria; 3- Leibniz Institute DSMZ - Germ an Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany. Abstract _ The goal of this study was to investigate the biodiversity of actinobacteria in Algerian Saharan soils by using a polyphasic taxonomic approach based on the phenotypic and molecular studies (16S rRNA gene sequence analysis and DNA-DNA hybridization ). A total of 323 strains of actinobacteria were isolated from different soil samples, by a dilution agar plating method, using selective isolation media without or with 15-20% of NaCl (for halophilic strains). The morphological and chemotaxonomic characteristics (diaminopimelic acid isomers, whole-cell sugars, menaquinones, cellular fatty acids and diagnostic phospholipids) of the strains were consistent with those of members of the genus Saccharothrix , Nocardiopsis , Actinopolyspora , Streptomonospora , Saccharopolyspora , Actinoalloteichus , Actinokineospora and Prauserella .
    [Show full text]
  • Microbial Extremophiles in Aspect of Limits of Life. Elena V. ~Ikuta
    Source of Acquisition NASA Marshall Space Flight Centel Microbial extremophiles in aspect of limits of life. Elena V. ~ikuta',Richard B. ~oover*,and Jane an^.^ IT LF " '-~ationalSpace Sciences and Technology CenterINASA, VP-62, 320 Sparkman Dr., Astrobiology Laboratory, Huntsville, AL 35805, USA. 3- Noblis, 3 150 Fairview Park Drive South, Falls Church, VA 22042, USA. Introduction. During Earth's evolution accompanied by geophysical and climatic changes a number of ecosystems have been formed. These ecosystems differ by the broad variety of physicochemical and biological factors composing our environment. Traditionally, pH and salinity are considered as geochemical extremes, as opposed to the temperature, pressure and radiation that are referred to as physical extremes (Van den Burg, 2003). Life inhabits all possible places on Earth interacting with the environment and within itself (cross species relations). In nature it is very rare when an ecotope is inhabited by a single species. As a rule, most ecosystems contain the functionally related and evolutionarily adjusted communities (consortia and populations). In contrast to the multicellular structure of eukaryotes (tissues, organs, systems of organs, whole organism), the highest organized form of prokaryotic life in nature is the benthic colonization in biofilms and microbial mats. In these complex structures all microbial cells of different species are distributed in space and time according to their functions and to physicochemical gradients that allow more effective system support, self- protection, and energy distribution. In vitro, of course, the most primitive organized structure for bacterial and archaeal cultures is the colony, the size, shape, color, consistency, and other characteristics of which could carry varies specifics on species or subspecies levels.
    [Show full text]
  • Put a Bow on It: Knotted Antibiotics Take Center Stage
    antibiotics Review Put a Bow on It: Knotted Antibiotics Take Center Stage Stephanie Tan , Gaelen Moore and Justin Nodwell * Department of Biochemistry, MaRS Discovery District, University of Toronto, 661 University Avenue, Toronto, ON M5G 1M1, Canada * Correspondence: [email protected]; Tel.: +1-416-946-8890 Received: 18 July 2019; Accepted: 9 August 2019; Published: 11 August 2019 Abstract: Ribosomally-synthesized and post-translationally modified peptides (RiPPs) are a large class of natural products produced across all domains of life. The lasso peptides, a subclass of RiPPs with a lasso-like structure, are structurally and functionally unique compared to other known peptide antibiotics in that the linear peptide is literally “tied in a knot” during its post-translational maturation. This underexplored class of peptides brings chemical diversity and unique modes of action to the antibiotic space. To date, eight different lasso peptides have been shown to target three known molecular machines: RNA polymerase, the lipid II precursor in peptidoglycan biosynthesis, and the ClpC1 subunit of the Clp protease involved in protein homeostasis. Here, we discuss the current knowledge on lasso peptide biosynthesis as well as their antibiotic activity, molecular targets, and mechanisms of action. Keywords: lasso peptides; ribosomally synthesized post translationally modified peptide (RiPP); antibiotic; target; mechanism of action 1. Introduction The natural products known as secondary or specialized metabolites are the source of thousands of antibiotics and other drugs that are critical for current medical practice. The majority of these compounds originated from the Actinobacteria, especially the streptomycetes, as well as lower fungi. The numbers of compounds produced by each species ranges from less than five in most cases to as many as 50 in the Actinobacteria and fungi.
    [Show full text]
  • Biochemical Methods for Preparation and Study of Peptide Natural Product Libraries
    BIOCHEMICAL METHODS FOR PREPARATION AND STUDY OF PEPTIDE NATURAL PRODUCT LIBRARIES Steven Robert Fleming A dissertation submitted to the faculty of the University of North Carolina at Chapel Hill in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Pharmaceutical Sciences in the Doctoral Program of the UNC Eshelman School of Pharmacy (Division of Chemical Biology & Medicinal Chemistry) Chapel Hill 2020 Approved by: Albert A. Bowers Michael B. Jarstfer Rihe H. Liu Kevin M. Weeks Leslie M. Hicks ©2020 Steven Robert Fleming ALL RIGHTS RESERVED ii ABSTRACT Steven Robert Fleming: Biochemical Methods for Preparation and Study of Peptide Natural Product Libraries (Under the direction of Albert Bowers) mRNA Display is an increasingly popular technique in pharmaceutical sciences to make highly diverse peptide libraries to pan for protein inhibitors. The current state of the art applies Flexizyme codon reprogramming with mRNA display to introduce unnatural amino acids for peptide cyclization and to further increase library diversity. Interestingly, ribosomally synthesized and post-translationally modified peptides (RiPP) are a unique class of natural products that transform linear peptides into highly modified and structurally complex metabolites. By combining RiPP biosynthesis with mRNA display, libraries of increasingly greater diversity can be achieved, and impending selected inhibitors will have natural product-like qualities, which we expect will allow these compounds to have better drug-like properties. Herein, we have developed a platform to measure RiPP enzyme modification of mRNA display libraries to show for the first time that RiPP enzymes can modify RNA linked peptide substrates. The platform may be extrapolated to many different RiPP enzymes and provides useful measurements to determine if a RiPP enzyme is promiscuous and effective to produce highly diversified peptides.
    [Show full text]
  • NOTE Studies of the Biological Characteristics of Some Halophilic and Halotolerant Actinomycetes Isolated from Saline and Alkaline Soils
    Actinomycetol. (2002) 17:06–10 VOL. 17, NO. 1 NOTE Studies of the Biological Characteristics of Some Halophilic and Halotolerant Actinomycetes Isolated from Saline and Alkaline Soils Shu-Kun Tang, Wen-Jun Li, Wang Dong, Yong-Guang Zhang, Li-Hua Xu and Cheng-Lin Jiang* The Key Laboratory for Microbial Resources of Ministry of Education, P. R. China, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091,Yunnan, China (Received Nov. 11, 2002 / Accepted Feb. 19, 2003) This study investigated the biological characteristics of 43 actinomycete isolates from saline and alkaline soils in Xinjiang, Hebei and Qinghai and representative strains of four genera under differing conditions of pH and varying concentrations of the mineral salts Na+, K+, Mg2+ and Ca2+. The results indicated that halotolerant actinomycetes have extensive adaptability to Na+, K+ and Mg2+, but that few strains can grow + even in low concentrations of CaCl2. Halophilic actinomycetes have extensive adaptability to Na ; for most, Na+ may be replaced by K+ or Mg2+, but not by Ca2+. Certain halophilic actinomycetes require Na+ to grow. It was also clear that the growth of all halophilic actinomycetes is dependent on different concen- trations of Na+, K+ or Mg2+. We believe that only kaliumophilic or magnesiumophilic or calciumphilic actinomycetes may be found in high salt environments. In addition, the range of pH values in which growth occurred was 6.0~10.0; optimum pH values were 7.0~8.0 for both halophilic and halotolerant actinomycetes. The distribution of halophilic actinomycetes was also found to be related to sample sources. INTRODUCTION mechanisms that provide tolerance to high concentrations of NaCl, whether having addiction with different ions, how Microorganisms found in extreme environments have pH and nutrition material of medium affect its growth and attracted a great deal of attention, due to the production by metabolic product.
    [Show full text]
  • Streptomonospora Alba Sp. Nov., a Novel Halophilic Actinomycete, and Emended Description of the Genus Streptomonospora Cui Et Al
    中国科技论文在线 http://www.paper.edu.cn International Journal of Systematic and Evolutionary Microbiology (2003), 53, 1421–1425 DOI 10.1099/ijs.0.02543-0 Streptomonospora alba sp. nov., a novel halophilic actinomycete, and emended description of the genus Streptomonospora Cui et al. 2001 Wen-Jun Li,1 Ping Xu,1 Li-Ping Zhang,1 Shu-Kun Tang,1 Xiao-Long Cui,1 Pei-Hong Mao,2 Li-Hua Xu,1 Peter Schumann,3 Erko Stackebrandt3 and Cheng-Lin Jiang1 Correspondence 1The Key Laboratory for Microbial Resources of Ministry of Education, PR China, Laboratory Cheng-Lin Jiang for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan [email protected] University, Kunming, Yunnan 650091, China 2Institute of Microbiology, Xinjiang Agriculture Academy of Sciences, Wulumuqi, Xinjiang 830000, China 3Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany A halophilic actinomycete, strain YIM 90003T, was isolated from a soil sample collected from Xinjiang Province, China, by using starch-casein agar with a salt concentration of 20 % (w/v), pH 7?0. The strain grew well on most media tested. No diffusible pigment was produced. Aerial mycelium and substrate mycelium were well developed on most media. The aerial mycelium formed short spore chains, bearing non-motile, straight to flexuous spores with wrinkled surfaces. The cell walls of strain YIM 90003T contained meso-diaminopimelic acid as the diagnostic diamino acid. Cell-wall hydrolysates contained galactose and arabinose. Menaquinone composition varied with the medium used for cell cultivation; on glucose-yeast extract medium supplemented with 10 % NaCl, the major menaquinone was MK-9(H4), while, on vitamin-enriched ISP 2 medium, the major menaquinones were MK-10(H2), MK-9(H8) and MK-10(H4).
    [Show full text]
  • Streptomonospora Litoralis Sp. Nov., a Halophilic Thiopeptides Producer Isolated from Sand Collected at Cuxhaven Beach
    Antonie van Leeuwenhoek (2021) 114:1483–1496 https://doi.org/10.1007/s10482-021-01609-4 (0123456789().,-volV)( 0123456789().,-volV) ORIGINAL PAPER Streptomonospora litoralis sp. nov., a halophilic thiopeptides producer isolated from sand collected at Cuxhaven beach Shadi Khodamoradi . Richard L. Hahnke . Yvonne Mast . Peter Schumann . Peter Ka¨mpfer . Michael Steinert . Christian Ru¨ckert . Frank Surup . Manfred Rohde . Joachim Wink Received: 7 April 2021 / Accepted: 23 June 2021 / Published online: 6 August 2021 Ó The Author(s) 2021 Abstract Strain M2T was isolated from the beach of Inferred from the 16S rRNA gene phylogeny strain Cuxhaven, Wadden Sea, Germany, in course of a M2T affiliates with the genus Streptomonospora. It program to attain new producers of bioactive natural shows 96.6% 16S rRNA gene sequence similarity to products. Strain M2T produces litoralimycin and the type species Streptomonospora salina DSM sulfomycin-type thiopeptides. Bioinformatic analysis 44593 T and forms a distinct branch with Strep- revealed a potential biosynthetic gene cluster encod- tomonospora sediminis DSM 45723 T with 97.0% 16S ing for the M2T thiopeptides. The strain is Gram-stain- rRNA gene sequence similarity. Genome-based phy- positive, rod shaped, non-motile, spore forming, logenetic analysis revealed that M2T is closely related showing a yellow colony color and forms extensively to Streptomonospora alba YIM 90003 T with a digital branched substrate mycelium and aerial hyphae. DNA-DNA hybridisation (dDDH) value of 26.6%. The predominant menaquinones of M2T are MK- 10(H ), MK-10(H ), and MK-11(H )([ 10%). Major Supplementary Information The online version contains 6 8 6 supplementary material available at https://doi.org/10.1007/ cellular fatty acids are iso-C16:0, anteiso C17:0 and C18:0 s10482-021-01609-4.
    [Show full text]
  • Structure-Activity Analysis of Gram-Positive Bacterium-Producing Lasso Peptides with Anti-Mycobacterial Activity
    www.nature.com/scientificreports OPEN Structure-Activity Analysis of Gram-positive Bacterium- producing Lasso Peptides with Anti- Received: 18 March 2016 Accepted: 30 June 2016 mycobacterial Activity Published: 26 July 2016 Junji Inokoshi, Nobuhiro Koyama, Midori Miyake, Yuji Shimizu & Hiroshi Tomoda Lariatin A, an 18-residue lasso peptide encoded by the five-gene clusterlarABCDE , displays potent and selective anti-mycobacterial activity. The structural feature is an N-terminal macrolactam ring, through which the C-terminal passed to form the rigid lariat-protoknot structure. In the present study, we established a convergent expression system by the strategy in which larA mutant gene-carrying plasmids were transformed into larA-deficientRhodococcus jostii, and generated 36 lariatin variants of the precursor protein LarA to investigate the biosynthesis and the structure-activity relationships. The mutational analysis revealed that four amino acid residues (Gly1, Arg7, Glu8, and Trp9) in lariatin A are essential for the maturation and production in the biosynthetic machinery. Furthermore, the study on structure-activity relationships demonstrated that Tyr6, Gly11, and Asn14 are responsible for the anti- mycobacterial activity, and the residues at positions 15, 16 and 18 in lariatin A are critical for enhancing the activity. This study will not only provide a useful platform for genetically engineering Gram-positive bacterium-producing lasso peptides, but also an important foundation to rationally design more promising drug candidates for combatting tuberculosis. Lasso peptides are microbial products ribosomally synthesized and post-translationally modified1,2. The struc- tural feature of lasso peptides is a macrolactam ring formed by a condensation reaction between the α -amino group of an N-terminal amino acid (Cys, Gly or Ser) and the side-chain carboxyl group of Glu or Asp at the positions 7, 8 or 92,3.
    [Show full text]
  • Genome-Based Taxonomic Classification of the Phylum
    ORIGINAL RESEARCH published: 22 August 2018 doi: 10.3389/fmicb.2018.02007 Genome-Based Taxonomic Classification of the Phylum Actinobacteria Imen Nouioui 1†, Lorena Carro 1†, Marina García-López 2†, Jan P. Meier-Kolthoff 2, Tanja Woyke 3, Nikos C. Kyrpides 3, Rüdiger Pukall 2, Hans-Peter Klenk 1, Michael Goodfellow 1 and Markus Göker 2* 1 School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom, 2 Department Edited by: of Microorganisms, Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Braunschweig, Martin G. Klotz, Germany, 3 Department of Energy, Joint Genome Institute, Walnut Creek, CA, United States Washington State University Tri-Cities, United States The application of phylogenetic taxonomic procedures led to improvements in the Reviewed by: Nicola Segata, classification of bacteria assigned to the phylum Actinobacteria but even so there remains University of Trento, Italy a need to further clarify relationships within a taxon that encompasses organisms of Antonio Ventosa, agricultural, biotechnological, clinical, and ecological importance. Classification of the Universidad de Sevilla, Spain David Moreira, morphologically diverse bacteria belonging to this large phylum based on a limited Centre National de la Recherche number of features has proved to be difficult, not least when taxonomic decisions Scientifique (CNRS), France rested heavily on interpretation of poorly resolved 16S rRNA gene trees. Here, draft *Correspondence: Markus Göker genome sequences
    [Show full text]
  • Lasso Peptides from Actinobacteria - Chemical Diversity and Ecological Role Jimmy Mevaere
    Lasso peptides from Actinobacteria - Chemical diversity and ecological role Jimmy Mevaere To cite this version: Jimmy Mevaere. Lasso peptides from Actinobacteria - Chemical diversity and ecological role. Biochemistry [q-bio.BM]. Université Pierre et Marie Curie - Paris VI, 2016. English. NNT : 2016PA066617. tel-01924455 HAL Id: tel-01924455 https://tel.archives-ouvertes.fr/tel-01924455 Submitted on 16 Nov 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Université Pierre et Marie Curie ED 227 : Sciences de la Nature et de l’Homme : écologie et évolution Unité Molécules de communication et Adaptation des Micro-organismes (MCAM) / Equipe Molécules de défense et de communication dans les écosystèmes microbiens (MDCEM) Lasso peptides from Actinobacteria Chemical diversity and ecological role Par Jimmy Mevaere Thèse de doctorat de biochimie et biologie moléculaire Présentée et soutenue publiquement le 14 novembre 2016 Devant un jury composé de : Véronique Monnet Directeur de recherche Rapporteur Jean-Luc Pernodet Directeur de recherche Rapporteur Jean-Michel Camadro Directeur de recherche Examinateur Marcelino T. Suzuki Professeur Examinateur Sergey B. Zotchev Professeur Examinateur Séverine Zirah Maître de conférences Directrice de thèse Yanyan Li Chargée de recherche Codirectrice de thèse Acknowledgements At first, I would like to thank Séverine Zirah and Yanyan Li, my PhD supervisor, and Prof.
    [Show full text]
  • Provided for Non-Commercial Research and Educational Use. Not for Reproduction, Distribution Or Commercial Use
    Provided for non-commercial research and educational use. Not for reproduction, distribution or commercial use. This article was originally published in the Encyclopedia of Microbiology published by Elsevier, and the attached copy is provided by Elsevier for the author’s benefit and for the benefit of the author’s institution, for non-commercial research and educational use including without limitation use in instruction at your institution, sending it to specific colleagues who you know, and providing a copy to your institution’s administrator. All other uses, reproduction and distribution, including without limitation commercial reprints, selling or licensing copies or access, or posting on open internet sites, your personal or institution’s website or repository, are prohibited. For exceptions, permission may be sought for such use through Elsevier’s permissions site at: http://www.elsevier.com/locate/permissionusematerial E M Wellington. Actinobacteria. Encyclopedia of Microbiology. (Moselio Schaechter, Editor), pp. 26-[44] Oxford: Elsevier. Author's personal copy BACTERIA Contents Actinobacteria Bacillus Subtilis Caulobacter Chlamydia Clostridia Corynebacteria (including diphtheria) Cyanobacteria Escherichia Coli Gram-Negative Cocci, Pathogenic Gram-Negative Opportunistic Anaerobes: Friends and Foes Haemophilus Influenzae Helicobacter Pylori Legionella, Bartonella, Haemophilus Listeria Monocytogenes Lyme Disease Mycoplasma and Spiroplasma Myxococcus Pseudomonas Rhizobia Spirochetes Staphylococcus Streptococcus Pneumoniae Streptomyces
    [Show full text]
  • Isolement Et Screening Des Actinobactéries À Partir Des Écosystèmes Extrêmement Salins
    REPUBLIQUE ALGERIENNE DEMOCRATIQUE ET POPULAIRE MINISTERE DE L’ENSEIGNEMENT SUPERIEURE ET DE LA RECHERCHE SCIENTIFIQUE Université Larbi Ben M'hidi Oum El Bouaghi Faculté Des Sciences Exactes et des Sciences de la Nature et de la Vie Département des Sciences de la Nature et de la Vie N ° d’ordre…… N ° de série…... Mémoire Présenté pour l’obtention du diplôme de MASTER Filière : Sciences biologiques OPTION : Microbiologie Appliquée Thème Isolement et screening des actinobactéries à partir des écosystèmes extrêmement salins Présenté par: MENASRIA Nour El Houda MEZIANE Amira Randa Rimas Devant le jury Président : DEROUICHE K. M.C.A Univ. Oum EL Bouaghi. Rapporteur : DJABALLAH C. M.A.A Univ. Oum EL Bouaghi. Examinateur : HAMAMES M. M.A.A Univ. Oum EL Bouaghi. Année universitaire : 2019-2020 Remerciements L’encadrement scientifique de ce travail a été assuré par Mr. DJABALLAH Chamsseddine, Maître-Assistant à l’université Larbi Ben M’Hidi Oum El Bouaghi. Nous tenons vivement à lui exprimer notre profonde reconnaissance et gratitude pour le temps qu’il a consacré pour sa patience, son suivi, sa compréhension, ses écoutes, ses qualités humaines et encore pour les précieuses informations qu’il nous a prodiguées avec intérêt. Nous tenons à remercier également les membres du jury Mr. DEROUICHE K. et Mr. HAMAMES M. pour avoir accepté d’examiner et d’évaluer ce travail. Nos remerciements les plus respectueux s’adressent aussi à Mr.ARABI Abed, Mr.Rouar Salim qui n’ont pas hésité à tout moment d’offrir leurs aides scientifiques, encouragements et fructueux conseils. Nous remercions tous nos chers professeurs qu’on a pu rencontrer pendant notre parcours universitaire.
    [Show full text]