ABCE1 Antibody Cat

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ABCE1 Antibody Cat. No.: 23-432 ABCE1 Antibody Western blot analysis of extracts of various cell lines, using ABCE1 antibody (23-432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 320-599 IMMUNOGEN: of human ABCE1 (NP_002931.2). TESTED APPLICATIONS: IF, WB WB: ,1:500 - 1:2000 APPLICATIONS: IF: ,1:50 - 1:200 September 26, 2021 1 https://www.prosci-inc.com/abce1-antibody-23-432.html POSITIVE CONTROL: 1) MCF7 2) Jurkat 3) 293T 4) HeLa 5) Mouse liver PREDICTED MOLECULAR Observed: 67kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: ABCE1 ABCE1, RLI, OABP, ABC38, RNS4I, RNASEL1, RNASELI, ATP-binding cassette, sub-family E ALTERNATE NAMES: (OABP), member 1, RNase L inhibitor, ribonuclease L (2', 5'-oligoisoadenylate synthetase- dependent) inhibitor, HP68, OK/SW-cl.40 GENE ID: 6059 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra- cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP BACKGROUND: subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. September 26, 2021 2 https://www.prosci-inc.com/abce1-antibody-23-432.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/abce1-antibody-23-432.html.

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Ribonuclease L Mediates the Cell-Lethal Phenotype of the Double-Stranded RNA Editing
1 2 Ribonuclease L mediates the cell-lethal phenotype of the double-stranded RNA editing 3 enzyme ADAR1 in a human cell line 4 5 Yize Lia,#, Shuvojit Banerjeeb,#, Stephen A. Goldsteina, Beihua Dongb, Christina Gaughanb, Sneha 6 Rathc, Jesse Donovanc, Alexei Korennykhc, Robert H. Silvermanb,* and Susan R Weissa,* 7 aDepartment of Microbiology, Perelman School of Medicine, University of Pennsylvania, 8 Philadelphia, PA, USA, 19104; b Department of Cancer Biology, Lerner Research Institute, 9 Cleveland Clinic, Cleveland, OH, USA 44195; c Department of Molecular Biology, Princeton 10 University, Princeton, NJ 08544 11 12 13 14 15 16 17 18 # These authors contributed equally to this work 19 * Corresponding authors 20 21 22 23 24 25 26 27 28 29 30 Abstract 31 ADAR1 isoforms are adenosine deaminases that edit and destabilize double-stranded RNA 32 reducing its immunostimulatory activities. Mutation of ADAR1 leads to a severe neurodevelopmental 33 and inflammatory disease of children, Aicardi-Goutiéres syndrome. In mice, Adar1 mutations are 34 embryonic lethal but are rescued by mutation of the Mda5 or Mavs genes, which function in IFN 35 induction. However, the specific IFN regulated proteins responsible for the pathogenic effects of 36 ADAR1 mutation are unknown. We show that the cell-lethal phenotype of ADAR1 deletion in human 37 lung adenocarcinoma A549 cells is rescued by CRISPR/Cas9 mutagenesis of the RNASEL gene or 38 by expression of the RNase L antagonist, murine coronavirus NS2 accessory protein. Our result 39 demonstrate that ablation of RNase L activity promotes survival of ADAR1 deficient cells even in the 40 presence of MDA5 and MAVS, suggesting that the RNase L system is the primary sensor pathway 41 for endogenous dsRNA that leads to cell death.
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