ABCE1 Antibody Cat. No.: 23-432 ABCE1 Antibody Western blot analysis of extracts of various cell lines, using ABCE1 antibody (23-432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 320-599 IMMUNOGEN: of human ABCE1 (NP_002931.2). TESTED APPLICATIONS: IF, WB WB: ,1:500 - 1:2000 APPLICATIONS: IF: ,1:50 - 1:200 September 26, 2021 1 https://www.prosci-inc.com/abce1-antibody-23-432.html POSITIVE CONTROL: 1) MCF7 2) Jurkat 3) 293T 4) HeLa 5) Mouse liver PREDICTED MOLECULAR Observed: 67kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: ABCE1 ABCE1, RLI, OABP, ABC38, RNS4I, RNASEL1, RNASELI, ATP-binding cassette, sub-family E ALTERNATE NAMES: (OABP), member 1, RNase L inhibitor, ribonuclease L (2', 5'-oligoisoadenylate synthetase- dependent) inhibitor, HP68, OK/SW-cl.40 GENE ID: 6059 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra- cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP BACKGROUND: subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. September 26, 2021 2 https://www.prosci-inc.com/abce1-antibody-23-432.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/abce1-antibody-23-432.html.