Status: Development of Mapping Popula ons
Sco E. Schlarbaum, University of Tennessee and Mark V. Coggeshall, University of Missouri
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 299
Proteales Sweetgum Liquidambar styraciflua Yes 0 No No 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No No 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Honeylocust Seed Collection
Isolated population of 12 trees on the Ames Plantation
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Honeylocust Mapping Population
Start of the 2012 growing season
End of the 2012 growing season
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Oak Molecular Mapping Plantations
Northern Red Oak
White Oak
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Oak Molecular Mapping Populations Nursery Phase
White Oak Tree 1
Overcup Oak Overcup Oak September, 2012 May, 2012 June, 2012 Overcup Oak White Oak
White Oak Swamp Chestnut Tree 2 Oak Comparative Genomics of Swamp Chestnut Hardwood Tree Species Oak http://www.hardwoodgenomics.org Yellow-Poplar Mapping Population
2011 Breeding
2011 Harvest 2012 Transplanting
2012 Germination Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Yellow-Poplar Mapping Population
2012 Transfer to Clemson
2012 Return to Knoxville and transplanting
2012 Move to Shadehouse Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Sugar Maple
Collecting samples for Isolated Parent Trees Romero-Severson Lab
Collecting Seed
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Redbay Material Collection
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Polyembryony in Northern Red Oak
Planting in raised nursery beds
Watauga Seed Orchard Transplanting polyembryonic acorns
Chromosome number will be checked
Growing in Shadehouse
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org It was a tough summer……. Record-breaking heat, coupled with only four cloudy days from May through August, led to the worst drought in Missouri since 1936. Evapotranspira on rates of 0.2 in/day were common. Rainfall equaled 4.2 inches from May 1st to the arrival of ‘Isaac’ in early September.
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Amborellales Fagales: Black walnut Magnoliales (Juglans nigra) Laurales
‘Schessler’ ‘Sparrow’ Monocots Angiosperms Proteales Vitales
Iden fica on of full sibs using paternity Malphigiales exclusion (PE) has been an integral part of Fabales
Eudicots Rosales the MU breeding program since 2009, resul ng in the iden fica on of 650+ full Fagales Myrtales sibs, including 299 ‘Sparrow’ x ‘Schessler’ eudiocts Brassicales Rosids Malvales seedlings (as of summer 2012).
Core Sapindales Cornales
Lamiales Asterids
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 299
Proteales Sweetgum Liquidambar styraciflua Yes 0 No No 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No No 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Fagales: Black Walnut (Juglans nigra) Mapping Popula on
Established in spring 2011, with addi onal seedlings planted in spring 2012.
Total of 299 full sibs successfully established to date. (325 originally) Total of 370 full sibs represented as 2011 DNA samples in ND library.
The size of this mapping popula on can be increased if desired, since addi onal o.p. seeds were collected in September 2012 for future paternity exclusion. 2012
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Fagales: Black Walnut (Juglans nigra)
In 2012, Paternity exclusion was completed by ND and all full sibs outplanted at MU.
Mul ple ssue types were collected from the ‘Sparrow’ MT for mRNA extrac on by PSU.
MT leaf and twig ssues were re-collected for BAC library construc on at CUGI.
Addi onal parental and full sib DNA was collected to facilitate marker selec on and gene c mapping work conducted at ND.
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 299
Proteales Sweetgum Liquidambar styraciflua Yes 0 No No 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No No 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Proteales: Sweetgum Amborellales (Liquidambar styraciflua) Magnoliales Laurales Monocots Angiosperms Proteales Vitales
Malphigiales Fabales Eudicots
Rosales
eudiocts Fagales Myrtales Brassicales Rosids Malvales Core Sapindales Due to seed quality issues (in 2011) and Cornales spring frosts in 2012, a new seed- bearing SWG mother tree (MT) has been Lamiales selected and seedlings will be produced
in 2013. Asterids
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 299
Proteales Sweetgum Liquidambar styraciflua Yes 0 No No 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No No 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Amborellales Lamiales: Green ash Magnoliales (Fraxinus pennsylvanica) Laurales
Monocots Angiosperms Proteales Vitales
Malphigiales Fabales
Eudicots Rosales
Fagales Myrtales The development of a GRA mapping eudiocts Brassicales Rosids Malvales popula on was part of the original NSF
Core Sapindales proposal related to the current project. Cornales
However, PE of the 700+ (1+1) seedlings currently available has not been ini ated Lamiales due to funding constraints. Asterids
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Lamiales: Green ash (Fraxinus pennsylvanica)
In 2012, We completed DNA sampling of the MT to facilitate gene c marker development at ND.
Completed DNA sampling of all puta ve males for future PE (if funded).
Mul ple ssue types were collected from the MT for mRNA extrac on by PSU.
Supplied 100 GRA seedlings to CU for stress tes ng.
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 299
Proteales Sweetgum Liquidambar styraciflua Yes 0 No No 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No No 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Amborellales Cornales: Black gum Magnoliales (Nyssa sylva ca) Laurales Monocots Angiosperms Proteales Vitales
Malphigiales Fabales
Eudicots Rosales
Fagales Myrtales Black gum was included in an eudiocts Brassicales earlier version of this project, and Rosids Malvales
Core Sapindales efforts to develop a mapping Cornales popula on began in 2009.
To date, we have ~125 seedlings Lamiales in hand, plus 200+ seeds from the
fall of 2012. Asterids Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 299
Proteales Sweetgum Liquidambar styraciflua Yes 0 No No 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No No 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Magnoliales: Yellow-poplar (Liriodendron tulipifera)
Open pollinated seedlings were grown from an isolated group of three trees in New Franklin, MO to serve as a back 1-1 seedlings (~300) are available for up to the UT mapping popula on, if plan ng in spring 2013. needed.
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 299
Proteales Sweetgum Liquidambar styraciflua Yes 0 No No 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No No 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Sapindales: Sugar maple Amborellales (Acer saccharum) Magnoliales Laurales Monocots Angiosperms Proteales Vitales
Malphigiales Fabales
Eudicots Rosales Fagales eudiocts Myrtales Brassicales Rosids Malvales
Core Sapindales Cornales
Lamiales We have collected seeds in fall
2012 as a backup to mapping Asterids popula on development at UT. Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org Mapping popula on development for selected species
No. Paternity No. Parent Seedlings DNA Exclusion Seedlings Host Order Species Selected in Hand Collected Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+
Magnoliales Yellow-poplar Liriodendron tulipifera Yes 550+ Yes No 0
Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+
Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0
Fagales Black walnut Juglans nigra Yes 0 Yes Yes 299
Proteales Sweetgum Liquidambar styraciflua Yes 0 No No 0
Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0
Cornales Black gum Nyssa sylva ca Yes 125+ No No 0
Comparative Genomics of Hardwood Tree Species
http://www.hardwoodgenomics.org