YMC HPLC Columns Applications Notebook

WA30000

HPLC Columns Applications Notebook Table of Contents

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YMC™ HPLC Column Quality Assurance...... 2

Guide to YMC™ Part Numbering System ...... 3

YMC™ Column Selection Guide ...... 4-7

HPLC Troubleshooting and Reference Guide...... 8-14

List of Applications...... 15-20

1. Vitamins ...... 21

2. Carbohydrates ...... 32

3. Nucleic Acids ...... 43

4. Amino Acids, Peptides, Proteins...... 48

5. Organic Acids, Fatty Acid Derivatives ...... 60

6. Food Additives ...... 62

7. Natural Products ...... 68

8. Steroids...... 77

9. Drugs, Metabolites ...... 87

10. Antibiotics...... 103

11. Pesticides, Environmental ...... 114

12. Miscellaneous ...... 119

Index of Analytes...... 128

1 YMC™ HPLC Column Quality Assurance

Waters Establishes Worldwide Distribution Network for YMC™ HPLC Columns

Waters Corporation announces worldwide distribution of the YMC™ HPLC A Product You Can Count On analytical and preparative column lines, a highly respected series of prod- ™ ucts that Waters has distributed since 1997. In expanding the distribution YMC products must pass internal performance criteria as raw materials, of YMC™ columns globally, Waters maintains exclusive distribution rights as synthesized silica particles, after they are bonded, and finally when for the YMC™ column product line in the Americas (North America, Central they are packed into a column. By setting tight standards, testing the raw America, South America and the Caribbean Islands). materials and precise control of silica gel synthesis, low metal levels of purity are achieved. Acceptance standards virtually eliminate problems with YMC...a Premium HPLC Packing Manufacturer nonspecific adsorption caused by trace contaminants in the silica gel. YMC has built its reputation on the supply of consistent, premium quality HPLC packing and columns. Control of the silica support is essential for product consistency and is of the highest priority to YMC. The silica supports are then functionalized with the Company’s proprietary bonding and endcapping chemistries to yield rugged, reproducible, high performance HPLC packings. Consistent Production Methods Year to Year Once a YMC product has been provided to customers for their chromatography, the methods used to produce that product will not be changed, as a matter of company policy. Even small changes in manufacturing methods to “improve” a product can lead to selectivity changes in methods for which that column is applied. Because YMC controls the production of its products from silica synthesis to packed column you can be assured that a method developed on a YMC™ column will be reproducible for as long as you conduct that method.

In-house manufacture gives YMC complete control over product quality and reproducibility. Proven procedures at every stage of manufacture guaran- tee consistency in silica purity, particle size, pore size, bonding and endcapping. Test chromatograms shipped with every column are merely the YMC-Pack™ Pro C18™ columns come with a detailed Certificate of final demonstration of a rigorously controlled product. Analysis for reliability and traceability.

US Pharmacopeia HPLC "L1" (C18) Column Designation Subclassification Testing by Brand Name*

Hydrophobicity Chelating Silanol Activity Shape Selectivity Column Brand Name (k, Ethylbenzene) (Tailing Factor (TF), Quinizarin) (k, Amitriptyline) (TF, Amitriptyline) (Bonding Density, mmol/m2) Pro C18™ 1.88 1.28 4.74 1.50 2.50

™ Hydrosphere C18 1.52 1.34 4.40 1.94 1.90 Pro C18™ RS 3.28 1.17 7.50 1.35 3.04 ODS-AL™ 1.79 1.52 13.59 2.81 2.56 ODS-A™ 1.89 1.53 5.00 2.40 2.66 ODS-AM™ 1.94 1.52 5.11 2.40 2.75 ODS-AQ™ 1.88 1.49 5.96 2.86 2.22 J’sphere™ ODS-H80 3.32 1.31 8.80 2.93 3.18 J'sphere™ ODS-M80 1.62 1.41 4.96 2.72 1.02 J’sphere™ ODS-L80 0.95 1.43 2.94 2.78 0.92

* For use in C18 Column Brand Name selection when referencing USP Monographs which specify the characteristics/performance of the C18 Column used in the method. Choose a brand as close as possible to the referenced column values for the best chance of duplicating the analytical results of the method.

Guide to YMC™ Part Numbering System

The YMC™ part numbering system is designed to help identify the product length, and column inner diameter. These are followed by fitting codes for being ordered. This system: internal use by Waters. The system is set out schematically below:

•embeds in the part number a description of the product that can be read by the user; Gel Code Hardware Code

• standardizes the YMC™ part numbers worldwide; and, Chemistry Pore Size in Particle Particle size Length Internal Code nm Shape in µm in mm diameter in mm • simplifies computerized stocking and ordering.

The table below will enable you to “read” your part numbers, and confirm Example: a semipreparative column with the part number that a part number you are about to use for ordering is exactly what you “OC12S052520WT” is in your stock. “Read” the part number as need. This should reduce ordering errors that can delay delivery of the cor- OC/12/S/05/25/20, and you can determine that this column is: rect column. Octyl 12nm (120Å) Spherical 5 µm 25 cm 20 mm I.D.

(C8) pores media particles long Gel Code & Hardware Code The part numbers are composed of a gel code, which describes the pack- Please do not use this table to write your own part numbers. Certain attrib- ing in the HPLC column, and the hardware code, which describes the col- utes that are available in some stationary phases are not available umn itself. The gel and hardware codes are further divided into short codes in others. If you are unsure of a part number, please check the catalog or that describe the chemistry, pore size, particle shape, particle size, column price list, ask your technical representative directly to confirm availability.

Chemistry Code Pore Size in Angstroms Particle Shape Particle Size in µm Length in mm Internal Diameter in mm

ODS-A™:AA60Å: 06 Spherical: S 3 µm: 03 10 mm: 01 0.2 mm: G0 ODS-AM™:AM80Å: 08 Irregular: I 4 µm: 04 20 mm: 02 0.3 mm: H0 ODS-AQ™:AQ120Å: 12 5 µm: 05 23 mm: G3 0.5 mm: J0 ODS-AL™:AL200Å: 20 6 µm: 06 30 mm: 03 1.0 mm: 01 PolymerC18™:PC300Å: 30 5/15 µm (10P): 11 33 mm H3 2.0 mm: 02 J’sphere™ ODS-H80: JH 1000Å A0 15 µm: 15 50 mm: 05 3.0 mm: 03 J’sphere™ ODS-M80: JM Proprietary or 10/20 µm: 16 75 mm: L5 4.0 mm: 04 J’sphere™ ODS-L80: JL unspecified: 99 15/30 µm: 21 100 mm: 10 4.6 mm: 46 YMCbasic™:BA 30/50 µm: 41 150 mm: 15 6.0 mm: 06

Octyl (C8): OC 50 µm: 50 250 mm: 25 8.0 mm: 08 Phenyl: PH 75 µm: 75 300 mm: 30 10 mm: 10

Butyl (C4): BU 150 µm: A5 500 mm: 50 20 mm: 20

TMS (C1): TM 350 µm: C5 1000 mm: A0 30 mm: 30 Cyano: CN Undesignated: 99 1500 mm: A5 50 mm (1000psi): 51 Amino: NH 2000 mm: B0 50 mm (2000psi): 52 PA-gel™:PA Irregular: 70 mm (1000psi): 71 Polyamine II: PB 5/20 µm: 12 70 mm (2000psi): 72 Silica: SL 10/25 µm: 17 100 mm (1000psi): A1 PVA-Sil™:PV 10/40 µm: 25 100 mm (2000psi): A2 Diol (NP): DN 25/40 µm: 32 150 mm (1000psi): B1 Diol (SEC): DL 40/63 µm: 52 150 mm (2000psi): B2 Protein-RP™:PR 63/210 µm: A4 200 mm (1000psi): C1 Pro C4 BS Pro C8 OS Pro C18™ AS 200 mm (2000psi): C2 Cartenoid CT Pro C18RS™ RS ™ Hydrosphere C18 HS

Choosing an HPLC Column YMC-Pack™ High Performance HPLC Columns High Performance Silica Supports YMC™ provides an extensive selection of packed columns to address your Spherical packing for long column life: YMC™ spherical packing pro- separation needs. Numerous specialty bonded phases are available to vides high performance with long column lifetimes. Spherical particles pack solve the most difficult separation problems. more uniformly than “spheroidal” or irregular supports. The greater mechanical stability of the column bed eliminates premature voiding. • Extensive selection of bonded phases available in five different porosities High surface area: The ultrapure YMC™ spherical supports have very high porosity, providing increased surface area for greater retention and • Column diameters from microbore to process scale increased resolution. In preparative applications, increased surface area • Column lengths from 10 to 2000 mm also means greater sample loading. The high sample loading, excellent •Available packed with 3 micron to resolution and long column life combine to provide higher productivity and 350 micron spherical particles economy in preparative separations.

• Columns individually tested for quality assurance State-of-the-Art Bonding and Endcapping High Reproducibility: Monomeric versus Polymeric Bonding: The bonding of ligands to the silica surface is often referred to as either “monomeric” or “polymeric”. With High purity synthetic spherical silica: ™ YMC synthetic silica supports con- monomeric bonding, the ligand has a single point of attachment via a tain only trace levels of metal impurities and organic contaminants. This monofunctional silane. The resulting surface provides superior efficiency ™ inert, chemically stable silica is the ideal support for YMC bonded phase and resolution for smaller molecules. chemistries and is an essential feature in YMC™ column reproducibility. The ultrapure YMC™ silica minimizes nonspecific adsorption and secondary With polymeric bonding, the ligands have multiple points of attachment via retention arising from silica contaminants. a trifunctional silane. (Polymerically bonded silica packing should not be confused with HPLC supports in which the support is a porous polymer.) The Every lot scrutinized: ™ Each lot of YMC*Gel is extensively tested for phys- selectivity differences between monomeric and polymeric bonding provide ical and chemical properties. After acceptance, the silica lot is bonded and options in method development. retested with a series of state-of-the-art chemical tests and specific chemical probes. These tests assure lot-to-lot reproducibility and confirm applicability Unique Endcapping Technology: Silica supports are the foundation of for the class of compounds for which the product is intended. The selectiv- most advanced HPLC packing, enabling these phases to tolerate the high ity and recovery of wide-pore bonded phases, for example, are batch-test- pressures of modern HPLC. When a silica particle is bonded, "residual ed with peptides and proteins. silanols" (unbonded surface silanol sites: -SiOH) can interact with polar samples and eluents. These interactions are desirable with some samples. With Every column tested: ™ Each YMC column is chromatographically tested basic samples and aqueous eluents, however, residual silanols can cause to assure that plate count, peak symmetry and general column perform- problems with peak symmetry and shorten column life. "End-capping" is a ™ ance meet YMC ’s high standard of quality. The column performance process by which residual silanols are bonded to minimize interaction with report supplied with every packed column fully documents the gel lot and solute molecules. The nature and completeness of the endcapping process test results. greatly influence the performance and quality of an HPLC column and, ulti- mately, the separation.

Choosing an HPLC Column

Impact of Pore Size Differences Packing Size Matched to Column Size YMC™ columns are available in five different pore sizes. Pore sizes are typ- Smaller particle packing performs best in smaller diameter columns, and ically matched to sample molecule sizes. Pore sizes can also be used to larger diameter packing excels in larger diameter columns. In general, adjust ligand density and thus, retention of a bonded phase, since the size 3 micron packing performs best in columns up to 10 mm I.D.; 5 micron of the pores also affects the total packing surface area in packing is best suited to columns up to 30 mm I.D.; and 10 micron pack- a packed column. ing is appropriate for columns up to 50 mm I.D. Columns of 100 mm I.D. or larger are generally packed with particles of 15 microns or larg- 60Å packing is available in bare silica for normal phase separations, and er in diameter. in diol-bonded phases for normal phase separations and aqueous size exclusion chromatography.

The 80Å J'sphere™ ODS and YMC-Pack™ Pro C18 RS™ pores and the 120Å pores available in the rest of the YMC™ product line are useful for the majority of separations. These pore sizes are suited for most organic compounds and for peptides less than 20,000MW. USP Chromatography Column 200Å pores, available in many YMC™ phases, can be used for virtually Classifications of YMC Columns any size organic compound, as well as for peptides and smaller proteins between 10 and 50 kD. The intermediate pore size provides access for USP Designation USP Description YMC, Inc. Phases bulkier molecules as well as higher surface area and higher preparative sample loading than 300Å media. ™ ™ L1 C18 bonded to 3-10 µm porous silica YMC-Pack Pro C18 YMC-Pack™ Pro C18™RS 300Å columns are appropriate for the analysis of large biomolecules and YMC-Pack™ ODS-A™ of organic compounds with excessive retention on smaller pore materials. YMC-Pack™ ODS-AM™ YMC offers 300Å porosity in a wide range of bonded phase chemistries. YMC-Pack™ ODS-AL™ YMC-Pack™ ODS-AQ™ J’sphere™ ODS series Wide Range of Column Sizes YMC-Pack™ Hydrosphere™ L3 5-10 µm porous silica YMC-Pack™ Silica Analytical Columns: To meet a range of HPLC situations, YMC™ offers ana- C bonded to 3-10 µm porous silica ™ lytical columns in diameters of 1.0 mm, 2.0 mm, 3.0 mm, 4.6 mm and L7 8 YMC-Pack C8 (Octyl) YMCbasic™ 6.0 mm in a variety of lengths. These analytical columns are available in ™ ™ all YMC*Gel chemistries. Typically, 5 micron spherical supports are used L8 NH2 bonded to 10 µm porous silica YMC-Pack NH2 (Amino) for most analytical situations, although other supports are also available. L10 CN bonded to 3-10 µm porous silica YMC-Pack™ CN (Cyano/Nitrile) Semipreparative Columns: YMC™ offers three different semipreparative L11 Phenyl bonded to 5-10 µm porous silica YMC-Pack™ Phenyl column sizes (10, 20 and 30 mm I.D.) for scaling up separations to gram L13 Trimethylsilane bonded to 3-10 µm porous silica YMC-Pack ™TMS quantities. Semipreparative columns are typically packed with 5 or 10 L20 Dihydroxypropane (Diol) bonded to 3-10 µm silica YMC-Pack™ Diol micron packings or with 15 micron high performance preparative media. 32-63 µm semi-rigid hydrophilic vinyl polymer gel ™ YMC semiprep columns packed with 5 or 10 micron media provide ana- L24 YMC-Pack PVA-Sil lytical grade separations at semiprep scale. L26 C4 bonded to 5-10 µm porous silica YMC C4 (Butyl) YMC-Pack™ Protein-RP Preparative Columns: Using spherical packing in YMC™ preparative L27 30-50 µm porous silica YMC-Pack™ Silica (S30/50) columns facilitates scale-up to preparative loads. High performance 10, 15, 25 and 50 micron spherical particles are available to address any L33 pH stable, silica-based GFC for 4-400KD proteins YMC-Pack™ 200Å Diol preparative need. Scale-up from analytical through semiprep to preparative column sizes is simplified by using the same bonded phase Unclassified C30 bonded to moderate-pore YMC30 chemistries throughout. YMC Phases porous silica The Carotenoid Column

™ ™ C18 bonded to rigid porous polymethacrylate YMC-Pack PolymerC18 Primary amines in polymer resin over porous silica YMC-Pack™ PA Secondary and tertiary amines in polymer resin YMC-Pack™ Polyamine II over porous silica

*YMC-Pack™ Pro C18™ utilizes a novel Lewis-Acid-Lewis Base endcapping procedure which gives the maximum surface coverage and inertness.

Characterization of Reversed-Phase Packings — The hydrophobicity increases fundamentally with the chain length. For example, you will find most C packings on the middle part of the chart, a Tool for Column Selection 8 and most C18 packings on the right side of the chart. The silanophilic inter- It is clear that the final properties of a reversed-phase packing depend on action is measured at neutral pH (pH 7.0, which allows for the de-proto- many different parameters of bonded-phase design and on the details of nation of the silanol group creating a negatively charged-- cation exchange the manufacturing process of the different packings. To condense this large surface). Packings based on high-purity silicas that are well endcapped body of data into information that is useful to the chromatographer, it is show a low silanol group activity and are found on the lower side of the important to focus on the most relevant properties of reversed-phase pack- graph. Embedded polar group ligands are found there also because they ings. There are two basic properties of a reversed-phase chromatographic minimize silanol interaction. These are the packings that are preferentially packing. One is the strength of the hydrophobic interaction which can eas- used for basic analytes. ily be measured by the retention factor of a purely hydrophobic analyte. On the upper end of the y-axis, you find packings with a high silanol activ- The second property is the strength of the silanophilic interaction. This can ity. These are typically based on either lower purity silica and/or are not best be measured by using the relative retention between an analyte that endcapped. These packings can be used to provide a different selectivity interacts by hydrophobic and silanophilic interaction and an analyte that than packings with a low silanol activity, but they usually exhibit tailing for interacts by hydrophobic interaction only. This has been done for many of basic analytes at neutral mobile phase pH. the packings that can be found in this catalogue as well as for some others, for which the relevant information was available. You can select the type of packing most suitable for your separation based on this graph. If your analytes are basic and exhibit strong interactions with A chromatographic test was developed so that all columns could be eval- surface silanols, select packings on the lower part of the graph. If you are uated under controlled conditions for these two chromatographic proper- looking for a significant difference in polar selectivity, you should select ties. Two marker analytes were chosen. Acenaphthene as the hydropho- packings that are located in different sections of the chart. If you are look- bic, neutral marker, and amitriptyline as the basic, silanophilic interaction ing simply for differences in hydrophobicity, use packings that are far away marker. The information is summarized clearly in the chart below. The x-axis from each other on the hydrophobicity axis. represents the hydrophobicity of a packing, and the y-axis is the silanophilic interaction of the packing. Note: The actual value obtained from the chromatographic evaluation is shown by the diamond symbol.

Selectivity Chart of Reversed-Phase Columns

4 Waters Spherisorb® Phenyl 3.5

3 Waters Spherisorb® CN Waters Spherisorb® ODS-1

2.5 Resolve™ C Nova-Pak® CN HP 18 Waters Spherisorb® ODS-2

2 µ ™ Bondapak C18 YMC-Pack™ ODS-AQ YMC™ J'Sphere™ ODS L80 1.5 Waters Spherisorb® ODSB ® Nova-Pak Phenyl YMC™ Hydrosphere™

™ ™ ™ ™ ™ YMC-Pack CN C18 YMC J'Sphere ODS-M80 YMC J'Sphere ODS-H80 ™ YMCbasic ® 1 Nova-Pak C18 ™ ™ ® ™ YMC-Pack ProC4 Nova-Pak C8 Atlantis dC18 ™ ™ ® YMC-Pack ProC18 RS XTerra® Phenyl Symmetry C8

Selectivity ™ 0.5 Symmetry300 C4 ® ™ ™ XTerra MS C8 YMC-Pack ODS-A ® XTerra MS C18 ® ™ ™ ™ YMC-Pack ProC8 Symmetry300 C18 Symmetry C18

™ SymmetryShield RP8 0 ™ SymmetryShield RP18

, Amitriptyline/Acenaphthene) ® ® XTerra RP18 α -0.5 XTerra RP8 YMC-Pack™ ProC18™ YMC™ PolymerC18™ (In -1 -2.00 -1.00 0.00 1.00 2.00 3.00 4.00

Hydrophobicity (In K, Acenaphthene)

Choosing a YMC-Pack™ Column for Your Separation

The following chart is a general guide for choosing a YMC-Pack™ column. Please contact our technical services for more in-depth recommendations.

Analyte Separation Analyte Solubility Mechanism Class YMC-Pack™ Column

GENERAL...... Pro C18, Pro C18RS™, ODS-A™, ODS-AQ™, Octyl, Hydrosphere™ Acids, Organic...... Pro C18™,Pro C18RS™, ODS-AQ™, Hydrosphere™ Acids, Amino (free)...... ODS-AQ™ Acids, Amino (derivatized)...... Pro C18™, Pro C18RS™, ODS-AQ™, YMCbasic™, ODS-A™, ODS-AM™, Hydrosphere™ Amines, Organic...... Pro C18™, Pro C18RS™, YMCbasic™, PolymerC18™ Amines, Quaternary (pH>7.5)...... PolymerC18™ Carbohydrates...... Polyamine II, PA-gel™, Amino Chelators...... Pro C18™, Pro C18RS™, PolymerC18™ Reversed- Drugs, Basic ...... Pro C18™, Pro C18RS™, YMCbasic™ Phase Dyes...... ODS-A™, ODS-AM™ Explosives ...... J’sphere™ ODS-H80 Metabolites ...... ODS-AQ™, YMCbasic™, Pro C18™, Hydrosphere™ Nucleosides ...... ODS-AQ™, Hydrosphere™ - Nucleotides...... ODS-AQ™, Hydrosphere™ Water Oligonucleotides...... ODS-AQ™, Hydrosphere™ Soluble Peptides...... Peptide Columns (ODS-AQ™, YMCbasic™), Hydrosphere™ Proteins...... Protein-RP™, Wide-pore columns, Butyl Sugars...... Polyamine II, PA-gel™, Amino Tricyclic Antidepressants...... Pro C18™, Pro C18RS™ Vitamins, Water Soluble ...... ODS-AQ™, Hydrosphere™

Oligosaccharides...... Diol Size Peptides...... Diol Exclusion Proteins...... Diol

Ion-Pairing Acids, Organic...... PolymerC18™, Pro C18™, Pro C18RS™, J’sphere™ ODS-H80 Ion- Amines, Organic...... PolymerC18™, Pro C18™, Pro C18RS™, J’sphere™ ODS-H80 Suppression

GENERAL...... Diol, PVA-Sil™, Silica Aflatoxins...... Silica Normal Steroids ...... Diol, PVA-Sil™ Phase Triglycerides...... Diol, PVA-Sil™ Vitamins, Fat Soluble ...... Diol, PVA-Sil™ Non- Water Aflatoxins...... Octyl, J’sphere™ ODS-M80, L80 Soluble Carotenoids ...... YMC30 Carotenoid Reversed- Fatty Acids ...... Octyl, Phenyl Phase Polyaromatics (PAH) ...... Phenyl, Octyl, YMC30 Vitamins, Fat Soluble ...... ODS-A™

© 2003 Waters Corporation.Waters is a trademark of Waters Corporation. YMC™ is a trademark of YMC™, Co.,Ltd. 7 HPLC Troubleshooting and Reference Guide chelating compounds , when alternative selectivitiy or general purpose phase ™ high and low pH samples 120Å: Non-polar peptides pharmaceuticals, amino acids Basic molecules w/o modifiers; proteins: general purpose phase Pharmaceuticals, organic amines, C18 Fat-soluble vitamins, steroids, fats 200/300Å: proteins/biopolymers pharmaceuticals, quaternary amines mixed polarity peptides, amino acids aggressive pH conditions are required Phenols, anilines, peptides in high pH, Separation of highly aqueous samples, Protein separations especially at low pH nonpolar peptides, fats, basic molecules Pro ailing compounds; organic acids, antibiotics, Natural products; peptides, pharmaceuticals T Organic bases, peptides, steroids, PAHs; difficult Organic bases, peptides, steroids, PAHs; Pharmaceuticals, vitamins, amino acids, peptides; Basic compounds, pharmaceuticals, an ideal compliment to 7 17 17 16 22 3.5 varies varies varies varies 14, 10 7, 5, 3 11, 7, 4 (H, M, L) 17, 11, 7 22, 14, 9 C18=10% 80 80 — 120 120 120 wide pore wide pore 120, 200 120, 200, 300 120, 200, 300 120, 200, 300 5 5 4 5, 15 6, 10, 50 30-50, 50 30-50, 50 30-50, 50 3, 5, 10, 15 3, 5, 10P, 15 3, 5, 10P, 25, 30-50, 50 3, 5, 7, 10P, 15 3, 5, 7, 10P, 3, 5, 7, 10P, 15 3, 5, 7, 10P, 3, 5, 10P, 15, 25 3, 5, 10P, 25, 30-50, 50, 75 3, 5, 7, 10P, 15, 25 3, 5, 7, 10P, 15, 25 3, 5, 7, 10P, 2-8 2-7 2-7 2-7 2-7 2-7 2-6 1-10 2-12 1.5-7 2-6.3 2-7(M,L) 1.1-9.3(H) L1 L1 L1 L1 L1 L1 L1 L7 L7 — L26 L26 18 18 18 Liquid Chromatography Products ™ C18 ™ Pro interactions very inert to RP: partition RP: partition RP: partition RP/Partition mixed mode RP: partition, RP: partition, RP: partition, RP: partition, RP: partition, RP: partition, RP: partition, low pH stable π most inert C unique selectivity most versatile C with alternative selectivity RP: partition, most inert C 8 C ≤ 18 18 18 C C Phase Principal USP pH Range Particle Size Pore Size Carbon noted) Mechanism No. ** (%C) w/hydrophilic Classic C Octylsilane Butylsilane -functionalized endcapping Butylsilane, neutral silica on ultrapure silica, nonendcapped 18 18 well endcapped well endcapped Alkylsilanes fully endcapped fully endcapped fully end-capped super endcapped polymethacrylate C C 18 acid-base endcapping bonded to ultrapure silica with polymeric bonding to C ultrapure silica with Lewis 18 18 C C w/Lewis acid-base endcapping ™ ™ ™ ™ ™ ™ C18 ODS-A ODS-AL Pro ODS-AM Product (silica unless Separation Class (room temp) (µm spherical) (Angstroms) LOAD Applications Typical J’sphere Protein-RP *ODS-AQ C8 (Octyl) C4 (Butyl) * *YMCbasic * ProC18 RS *PolymerC18 Quick Reference Guide - YMC

8 © 2003 Waters Corporation.Waters is a trademark of Waters Corporation. YMC™ is a trademark of YMC™, Co.,Ltd. HPLC Troubleshooting and Reference Guide of pesticides pharmaceuticals pharmaceuticals carotenoids isomers column washing OK vitamins, tocopherols parafins, olefins, anions with NP solvents, vitamins sugars, maltose and lactose Polar and nonpolar geometric Organic bases, lipids, retinoids polarity peptides, organic acids Aromatics, peptides and proteins, analytes, carotenoids, metabolites More stable than amino over time; Natural products, lipids, metabolites Sugars; very stable; high pH (<8.5) Aqueous size separations; biomolecules, Sugars, saccharides, tocopherols, phenols, Separation of highly aqueous samples, mixed Small organic molecules to polymers, fat-soluble Multifunctional compounds, nonpolar compounds RP/NP: phenols, pharmaceuticals, environmental phospholipids, carotenoids; OK with polar solvents 3 20 12 — — — — — 9, 3 7, 3 N/A varies varies varies 4, 3.5, 3 120 120 120 120 wide pore 120, 300 120, 300 120, 300 120, 200, 300 60, 120, 200, 300 60, 120, 200, 300 60, 120, 200, 300 5 5 5 3, 5 3, 5, 15 30-50, 50 30-50, 50 75, 150, 350 25, 30-50, 50 25, 30-50, 50 5, 10P, 15, 25 5, 10P, 5, 10, 15, 25, 50 5, 10P, 15, 25, 50 5, 10P, 3, 5, 7, 10P, 15, 25 3, 5, 7, 10P, 15 3, 5, 7, 10, 10P, 3, 5, 7, 10P, 15, 25, 50, 3, 5, 7, 10P, 3, 5, 7, 10, 10P, 15, 3, 5, 7, 10, 10P, NA 2-7 2-7 2-7 2-6 2-7 2-7 2-7 2-9 2-9 2-8 2-9.5 L3 L8 L1 — — — L13 L11 L10 L24 L20 L33 NP: RP/NP: interactions H bonding H bonding H bonding separation RP: partition RP: partition Aqueous size RP: partition, π RP: most polar, NP: adsorption, NP: adsorption, NP: adsorption, High purity silica NP: silica alternative intermediate polarity NP: good alt to silica, NP: good alt to silica, adsorption/H bonding H bonding/adsorption H bonding, ion exchange 30 C Diol phase Propyl amine Phase Principal USP pH Range Particle Size Pore Size Carbon noted) Mechanism No. ** (%C) endcapped endcapped endcapping with hydrophilic Spherical or irregular gel functionalities rimethyl silane Monomeric CN, T bonded to silica Polyvinyl alcohol 18 Monomeric phenyl, C amine functionalities Polyamine: 2° amine 1, 2-dihydroxypropane Polyamine: 1°, 2°, 3° 18 ™ C ™ ™ ™ ™ (Amino) (TMS) Diol SEC Silica Phenyl 2 1 (PBMN) Column *PA gel *PA *PVA-Sil Product (silica unless Separation Class (room temp) (µm spherical) (Angstroms) LOAD Applications Typical C CN (Cyano) NH Aqueous GPC/ *Polyamine II *The Carotenoid Hydrosphere

HPLC Troubleshooting

Although there is no substitute for experience or consulting with an “expert,” here are some tips and tricks, some answers to the most com- monly-asked technical inquiries, and some key questions to ask to help diagnose your problem.

Poor Peak Shape, Loss of Resolution, or Peak Splitting Probable Causes: Mechanical fouling. Column void. Sample overload. Sample-mobile phase incompatibility. Dead volume from improperly assembled fittings and connectors. Adsorption (active sites).

Key Questions: Are all peaks equally affected? Did the peak shape start out fine but degrade over time? Has the back pressure increased?

Be certain that…. The mobile phase is the proper pH for your analytes. Your analytes are soluble in the mobile phase. You are not overloading the column with too much sample. Ensure that the sample solvent is not too strong for the mobile phase. All connecting fittings are well-seated (see Column Hardware Section.

First Pass Solution: Make sure your column is properly connected with appropriate fittings for perfect, zero dead volume connections. Retest the column under our QA test procedure (shown on the report shipped with the column).

If the column looks fine, then the problem is specific to your analyte or system. If the column has poor performance, refer to the “Cleaning/regenerating columns” procedures at the end of this section.

High Pressure Probable Causes: Restricted flow-path.

Be certain that…. All components in the mobile phase and sample are compatible with one another, filtered, and that precipitation can- not occur.

First Pass Solution: Replace column with a union and measure total system pressure. If pressure without the column is high, disconnect all connections starting at the end of the flow path until you’ve isolated the section giving high pressure. If pressure without the column is acceptable, attempt to remove particulate matter from the inlet frit by running the column in reverse with the outlet disconnected from the detector. If pressure is still high, refer to the “Cleaning/regenerating columns” procedures at the end of this section. Use inline filters or improve your sample preparation procedure to remove particulates and contaminating matrix components.

Retention Time or Selectivity Variation Probable Causes: Variable pump flow, temperature variations, or solvent mixing. Mobile phase preparation technique. Chemical variability in column.

Be certain that…. Comparisons are being made under identical system, mobile phase, and sample conditions. The columns are properly equilibrated. Columns have not been exposed to conditions that could change them. Equilibration of columns using Ion-Pairing reagents has to be well controlled.

Key Questions: Is the void time consistent? Using the same sample and mobile phase bottle on the same day, how do the two columns in question compare?

First Pass Solution: Test the columns under our QA conditions to make sure they are still in good condition. Gather chromatograms from the columns in question, including details on mobile phase, system, and sample conditions, column serial number, and how they’ve been used in the past. Depending on use conditions, older columns can perform differently due to chemical changes to the packing material.

Retention Time Drift Probable Causes: System not equilibrated. Adsorption (active sites). Temperature changing.

Be certain that…. All components in the mobile phase and sample are compatible with one another. The column is properly equilibrated. Drift is not caused by inconsistent column temperature or flow rate.

Key Questions: Is drift sample- or mobile phase-dependent? Do all peaks drift equally, or do certain ones drift more than others? Is the void time stable? Is this a Normal Phase Method?

First Pass Solution: Retest after cleaning the column according to “Cleaning/regenerating columns” procedures at the end of this section, and re-equilibrating in fresh mobile phase.

For many Normal Phase applications, varying moisture levels in the mobile phase will cause the active packing material to change. Control the moisture level.

Baseline Unstable or Noisy Probable Causes: Column not equilibrated in mobile phase. Pump delivering unstable flow. Leaks. Low detector lamp energy. Air bub- ble, debris, or contamination in detector cell. Mobile phase not being mixed properly. Mobile phase contaminated. Purity of mobile phase components not high enough. Elution from column of strongly-adsorbed material.

Be certain that…. Pump is operating properly. Detector cell is clean. You are using HPLC-grade mobile phase components. Your water is HPLC-grade and is fresh. Your mobile phase is properly degassed.

Key Questions: Does replacing the column solve the problem?

First Pass Solution: Clean the column according to “Cleaning/regenerating columns” procedures at the end of this section, and re-equilibrate in fresh mobile phase.

Poor Sensitivity Probable Causes: Detector lamp energy low. Wrong wavelength. Detector cell contaminated. Detector or integrator settings or calibration incorrect. Analyte degraded or not in solution. Injection volume inconsistent. Too much extra-column volume. Adsorption (active sites).

Be certain that…. HPLC system (detector and injector) is clean, calibrated, and operating properly. All liquid connections are zero dead volume.

Key Questions: Does a similar column give better sensitivity?

First Pass Solution: If you can get good sensitivity from another similar column, adsorption may be occurring. Retest after cleaning the column in question according to “Cleaning/regenerating columns” procedures at the end of this section.

Ghost Peaks Probable Causes: Carryover from prior injections. Contamination of column or system. Pump oil leaking into system. Late-eluting peak from previous sample.

Be certain that…. The ghost peak is not electronic noise (test with pump turned off).

Key Questions: What samples have you previously injected onto the column? Does the peak diminish with each injection, stay the same, or get larger? Does it occur only at low UV wavelengths (210nm) with Methanol?

First Pass Solution: Make several consecutive 0 µL injections (turn injector valve).

➜ If peak decreases with each injection, the contamination is from a previous sample injection. Clean the entire system (excluding the column) with isopropanol. On a separate system, clean the column according to “Cleaning/regenerating columns” procedures at the end of this section. Add a strong solvent flush at the end of the run to your analysis conditions to remove late eluting compounds.

Ghost Peaks (continued) ➜ If peak stays the same size with each injection, the contamination is from the mobile phase. Ion-pair reagents and water should be particularly suspect. Clean the system with isopropanol. On a separate system, clean the column according to “Cleaning/regenerating columns” section below. Replace every component of your mobile phase, including glassware and solvent reservoirs, and check pump to make sure it isn’t leaking oil into the lines.

➜ If no peak is seen with a 0 µL injection, the ghost peak is in your sample. Add a strong solvent flush at the end of the run to your analysis conditions to remove late eluting compounds. Make sure sample is filtered and that all components are soluble in the mobile phase and sample solvent.

If the peak disappears at 254 nm, then you have an oxygen-methanol complex which appears as a peak at 210 nm. Because the mobile phase is degassed, there is no oxygen present until you inject your sample which has oxygen dissolved in the injection solvent.

Negative Peaks (dip below the baseline) Probable Causes: Detector or integrator settings or calibration incorrect. Mobile phase has higher UV absorbance or greater refractive index than analyte. Refractive index of sample solvent differs too much from the mobile phase. Ion pairing mobile phase.

Be certain that…. The negative peak is not electronic noise (test with pump turned off).

First Pass Solutions: 1. Make a 0 µL injection (turn injector valve). If the negative peak is seen, then it is due to the refractive index change caused by the momentary stop in flow or increase in pressure when the valve switches.

2. Make injections of sample solvent only.

➜ If negative peak is seen, your sample solvent has less absorbance or a drastically different refractive index than your mobile phase. Change all of your mobile phase components, making sure they are fresh, HPLC grade (especially ion-pair reagents and your water), and have no absorbance at your analysis wavelength.

➜ If you don’t see the negative peak, then there is a component in your sample that has less absorbance or a drastically different refractive index than your mobile phase. Adjust the detector wavelength. Change all of your mobile phase components, making sure they are fresh, HPLC grade (especially ion-pair reagents and your water), and have no absorbance at your analysis wavelength.

Key Calculations Parameter Units Equation

1 Resolution none R = 1/4 N /2 (α - 1) [k/(1+k)]

R = 2(Tr2 – Tr1)/(w2 + w1) where Tr is retention time, and w is baseline peak width

2 Void volume (V0) mL V0 = (0.65)π(d/2) L where d is column I.D. in cm, and L is column length in cm

Void time (T0) minutes T0 = V0 /F where F is flow rate in mL/min

Capacity factor (k) none k = (Tr - T0)/ T0 where Tr is retention time in minutes

Selectivity (α) none α = k2/k1

2 Efficiency (N) plates/column N = 16(Tr/w) where w is baseline peak width (Tr and w must have the same units)

Linear velocity (u) cm /sec u = L/T0

where L is column length and T0 is void time in seconds

Physical Constants for Some Common HPLC Solvents

Solvent Formula Solubility in Boiling Density UV Cutoff Viscosity at Polarity water (%w/w) Pt. (°C) (g/mL) (nm) 20 °C (cP) Index

Acetic acid CH3COOH fully soluble 117.9 1.0492 230 1.26 6.2 Acetone CH3COCH3 fully soluble 56.2 0.7899 330 0.32 5.1 Acetonitrile CH3CN fully soluble 81.6 0.9483 190 0.37 5.8 Carbon tetrachloride CCl4 0.08 76.5 1.5940 263 0.97 1.6 Chloroform CHCl3 0.82 61.7 1.4832 245 0.57 4.1 Dichloromethane CH2Cl2 1.60 40 1.3266 235 0.44 3.1 1,4-Dioxane C4H8O2 fully soluble 101 1.0337 215 1.54 4.8 Dimethylsulfoxide (DMSO) (CH3)2SO fully soluble 189 1.1014 268 2.00 7.2 Ethanol CH3CH2OH fully soluble 78.5 0.7893 210 1.20 5.2 Ethyl acetate CH3COOCH2CH3 8.70 77.1 0.9003 260 0.45 4.4 Diethylether CH3CH2COCH2CH3 6.89 34.5 0.7138 220 0.32 2.8 Heptane CH3(CH2)5CH3 <0.01 98.4 0.6837 200 0.39 0.0 Hexane CH3(CH2)4CH3 <0.01 69 0.6603 200 0.33 0.0 Methanol CH3OH fully soluble 65 0.7914 205 0.60 5.1 Methyl-t-butyl ether (MTBE) CH3OC((CH2)3CH3)3 4.8 55.2 0.7404 210 0.27 2.5 n-Propanol CH3CH2CH2OH fully soluble 97.4 0.8035 210 2.27 4.0 2-Propanol

(Isopropanol) CH3CH2(OH)CH3 fully soluble 82.4 0.7855 210 2.30 3.9 Tetrahydrofuran (THF)

(Tetramethylene oxide) C4H8O fully soluble 66 0.8892 215 0.55 4.0 Toluene (C6H5)CH3 0.05 110.6 0.8669 285 0.59 2.4 Water H2O -- 100 1.00 -- 1.00 9.0

Viscosity Curves for Aqueous Eluent Mixtures

Ethanol

Tetrahydrofuran 2 Methanol

Acetone

Acetonitrile iscosity at 20° C (cp) V 1

0 0 10 20 30 40 50 60 70 80 90 100 Volume % Water

Recommended Column Cleaning and Regenerating Procedures

Use the cleaning routine that matches the properties of the column and what you believe is contaminating it. Flush columns with 20 column volumes (e.g., 80 mL total for 4.6 x 250 mm column) of HPLC-grade solvents. Run columns in reverse flow direction, with the outlet disconnected from the detector. Cleaning efficiency is increased by increasing mobile phase temperature to 35-55 °C. If the column performance is poor after regenerating and cleaning, call us for additional support.

Silica-based particles: Non-polar-bonded phases

Polar Samples Non-polar Samples Proteinaceous Samples 1. water 1. isopropanol Option 1: Inject repeated aliquots of DMSO 2. methanol 2. THF Option 2: gradient of 10 to 90% B where: 3. THF 3. dichloromethane A = 0.1% TFA in water

4. methanol 4. hexane B = 0.1% TFA in CH3CN 5. water 5. isopropanol Option 3: Flush column with 7M guanidine HCl, or 7M urea 6. mobile phase 6. mobile phase

Silica-based particles: Polar-bonded phases

Polar Samples Nonpolar Samples (aqueous mobile phases) (non-aqueous mobile phases) 1. water 1. chloroform 2. methanol 2. methanol 3. THF 3. dichloromethane 4. methanol 4. heptane or isooctane 5. water 5. isopropanol 6. mobile phase 6. mobile phase

Polymer-based particles

1. Flush column with mobile phase but omit buffers or salts, for example, just organic and water, acetonitrile is preferable 2. Run a gradient to 100% organic 3. Flush with twenty column volumes of THF 4. Flush with twenty column volumes of acetonitrile 5. Run a gradient back to starting mobile phase conditions, omitting buffers and salts 6. Re-equilibrate in mobile phase

14 © 2003 Waters Corporation.Waters is a trademark of Waters Corporation. YMC™ is a trademark of YMC™, Co.,Ltd. Click on the links below to go directly to that section of the notebook. To start a new search, click on List of Applications Bookmark to the left

List of Applications 1. Vitamins

Oil-soluble vitamins...... 21 Vitamin A fatty acid ester...... 21 Retinol...... 21 Carotene...... 21 β-Carotene ...... 22 Vitamin D ...... 22 Tocopherols ...... 23,24 Tocopherol and tocopherol acetate ...... 24,25 Vitamin K...... 25 A drink including vitamins...... 26 Water-soluble vitamins ...... 25,26,27,28,29 Allantoin and water-soluble vitamins ...... 29 Thiamine and its modifide compounds ...... 30 Riboflavin and related compounds ...... 30 Nicotinic acid and derivatives...... 30 Pantothenyl alcohol ...... 30 Coenzyme A and derivatives ...... 31 L-Ascorbic acid and erythorbic acid ...... 31 2,4-Dinitrophenylhydrazone of L-ascorbic acid ...... 31

2. Carbohydrates

Monosaccharides and disaccharides...... 32 Monosaccharides ...... 32 Unsaturated chondro-disaccharides ...... 33 Sugar alcohols ...... 33 Oligosaccharides ...... 33,34,35 Fructooligosaccharides...... 34 Oligosaccharides of soft drink ...... 34 Maltooligosaccharides ...... 35,36,37 Cyclodextrins ...... 37,38 Pyridylamino(PA)-Sugar chains...... 38,39 Polydextrose...... 40 Pullulans ...... 40,41 Chondroitin...... 41 Chondroitin sulfate A ...... 41 Chondroitin sulfate B ...... 41 Chondroitin sulfate C...... 42 Hyaluronic acid ...... 42 Heparin...... 42 Heparan sulfate ...... 42

© 2003 Waters Corporation.Waters is a trademark of Waters Corporation. YMC™ is a trademark of YMC™, Co.,Ltd. 15 Click on the links below to go directly to that section of the notebook. To start a new search, click on List of Applications Bookmark to the left List of Applications List of Applications

3. Nucleic acids

Keratan sulfate...... 43 Keratan polysulfate ...... 43 Nucleosides and bases...... 43 Nucleic acid bases ...... 43 Orotic acid...... 44 Nucleotides ...... 44,45,46 Oligonucleotides...... 46 Poly(A) ...... 46 Sugar nucleotides...... 46,47 Plasmid pBR322 restriction fragments...... 47

4. Amino acids, Peptides, Proteins

Amino acids...... 48 Taurine in nutritious drink...... 48 PTC-amino acids...... 48 PTH-amino acids ...... 48 NBD-amino acids...... 49 Peptides and proteins...... 49 Glutathione, oxidized and reduced form ...... 49 Angiotensins...... 49,50 Insulins ...... 50,51 Peptides ...... 51,52,53,55,56,59 Human immunoglobulins...... 53,54 Normal Human IgA...... 54 Cytochrome c...... 55 Peptide fragments from myoglobin...... 55 Glycine oligomers ...... 56 Casein ...... 56 Proteins for molecular weight marker...... 56,57,58 Proteins ...... 58 Human IgM(κ) myeloma protein ...... 54

5. Organic acids, Fatty acid derivatives

Organic acids ...... 60 Pyruvic acids ...... 60 Unsaturated fatty acid methyl esters ...... 61 Methyl linolenate and methyl γ-linolenate ...... 61 Triacylglycerols ...... 61

To startList of a Applications new search, click on List of Applications Bookmark to the left List of Applications

6. Food additives

Food preservatives ...... 62,67 p-Hydroxybenzoic acid esters...... 62,63 EDTA-chelates...... 63 Antioxidants ...... 63,64,65 Color additives...... 65 Annatto extract ...... 66 Aspartame in a soft drink...... 66,67 Stevioside and rebaudioside A...... 67 Glycyrrhizic acid...... 67

7. Natural products

Purine Alkaloids ...... 68 Alkaloids ...... 68 Chlorophyll a and b ...... 68 Crude Drugs (Glycosides) ...... 69 Paeoniflorin...... 69 Paeoniflorin in Toki-shakuyaku-san...... 70 Saponins from roots of rubus suavissimus S.Lee...... 70 Saikosaponins ...... 70 Ginsenosides ...... 71 Flavonoids ...... 71,72 Baicalin...... 72 Flavanone glycosides...... 72,73 Isoflavones from miso, fermented soybean paste ...... 73 Catechins ...... 73,74 2-Phenylethyl glycosides ...... 74 Sennosides ...... 74,75 Arbutin and related compounds ...... 75 Paeonol...... 76 Aflatoxins...... 76

8. Steroids

Steroids...... 77,80 Adrenocorticosteroids...... 77,78,79,80 Hydrocortisone and prednisone ...... 78 Androgens...... 81 Synthetic androgens ...... 81,82 Estrogens and progestins ...... 82 Estrogens...... 82,83 Norgestrel and ethinylestradiol ...... 83 Phenacyl esters of bile acids from pig gall ...... 83

© 2003 Waters Corporation.Waters is a trademark of Waters Corporation. YMC™ is a trademark of YMC™, Co.,Ltd. 17 Click on the links below to go directly to that section of the notebook. To start a new search, click on List of Applications Bookmark to the left List of Applications

List of Applications 8. Steroids (Cont.)

Ecdysones...... 84 Sterols ...... 84 Cardiac glycosides...... 85 Steroids from toad venom ...... 86

9. Drugs, Metabolites

Pain-killing drugs ...... 87,90 Indometacin ...... 87 A cough/cold medication...... 87 Ingredients in a cough/cold medication ...... 87,88 Analgesics ...... 88 Benzethonium chloride ...... 89 Benzalkonium chloride ...... 89 Ingredients in drug for rhinitis...... 89 Ingredients in an eye drop ...... 89 Codeine ...... 90 Collunarium...... 90 Naphazoline hydrochloride and chlorpheniramine maleate...... 90 Histamine antagonists ...... 91 Acidic non-steroidal anti-inflammatory drugs ...... 91,93 Antipsychotic drugs...... 92 Antiepileptics ...... 92 Tricyclic antidepressants...... 93 Calcium antagonists...... 94 Hypolipidemic drugs...... 95 Lovastatin...... 95 Antiarrhythmic drugs...... 94,97 Local anesthetics ...... 97 Diuretics ...... 96,97 Antineoplastic and related compounds ...... 96 Folic acid and its antagonists ...... 96 Nicotinic acid analogues...... 98,100 Basic compounds ...... 98 Disinfectants ...... 98 Acyclovir ...... 99 Carnitine chloride...... 99 Xanthine oxidase inhibitor and related compounds ...... 99 Anti-human immunodeficiency virus (HIV) agents ...... 100 Acidic and Basic drugs ...... 101 Chlorhexidine ...... 101 Purine catabolites ...... 102 Catecholamines ...... 102 Hippuric and methylhippuric acids...... 101,102

18 © 2003 Waters Corporation.Waters is a trademark of Waters Corporation. YMC™ is a trademark of YMC™, Co.,Ltd. Click on the links below to go directly to that section of the notebook. To start a new search, click on List of Applications Bookmark to the left List of Applications

List of Applications 10. Antibiotics

Antibacterial agents ...... 104 Sulfonamides ...... 103,104,105 Antibiotics ...... 105 Nalidixic acid antibiotics ...... 105,106 Cephalosporin antibiotics...... 106,107,108,109,110 Cefoxitin sodium...... 111,112 Penicillin antibiotics ...... 111 Amoxicillin...... 111 Cephalosporin C...... 112 Ceftriaxone ...... 112 Erythromycin antibiotic drugs...... 113 Tetracycline antibiotics ...... 113 Vancomycin ...... 113

11. Pesticides, Environmental

Copper 8-quinolinolate...... 114 Herbicides ...... 114 Atrazine derivatives...... 114 Triazine herbicides...... 114,115 Urea herbicides...... 115 Acid amide herbicides ...... 115 Carbamate herbicides ...... 115 Carbamate insecticides ...... 116 Fungicides...... 116 Imazalil...... 116 2,4-Dinitrophenylhydrazones of aldehydes and ketones ...... 117 Polyaromatic hydrocarbons...... 117,118

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List of Applications 12. Miscellaneous

Benzoic acid derivatives ...... 119,120 Methoxybenzoic acid isomers...... 119 Chlorobenzoic acid isomers ...... 119,122 Hydroxybenzoic acids...... 120 Resorcylic acid isomers ...... 120 Phenol derivatives ...... 121 Cresol isomers ...... 121,124 Polyphenols ...... 122 Dimethylphenol isomers...... 122 Aminoalcohols ...... 123 Urea and related compounds ...... 123 A nonionic detergent ...... 123 Polyethyleneglycols ...... 123 Metal-PAN chelates...... 124 Coordination compounds ...... 124 Hinokitiol...... 124 Phthalic acid isomers...... 125 Chlorophenol isomers ...... 125 Chloracetophene isomers ...... 125 Fullerenes ...... 126 Aromatic amines...... 126 Terphenyl isomers...... 127

Oil-soluble vitamins Vitamin A fatty acid ester

1. Menadione (Vitamin K3) 1. Retinol palmitate 2. Retinol (Vitamin A) (Vitamin A palmitate) 3. Retinol acetate

4. Menaquinone (Vitamin K2) 5. δ −Tocopherol

6. Ergocalciferol (Vitamin D2)

7. Cholecalciferol (Vitamin D3) 8. α−Tocopherol (Vitamin E) 9. α−Tocopherol acetate

10. Phylloquinone (Vitamin K1)

(G910415B) (G910523T)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ SIL (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : SL12S051546WT Eluent : acetonitrile Eluent : cyclohexane/dichloromethane/acetic acid (100/0.5/0.5) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30˚C Temperature : 30 ˚C Detection : UV at 280 nm, 0.08 AUFS Detection : UV at 350 nm, 0.32 AUFS Injection : 15 µL (0.01~0.21 mg/mL) Injection : 5 µL (1.0 mg/mL)

Retinol Carotene

1. Retinol 1. α - Carotene 2. ß - Carotene

(A970708A) (G910523Q)

™ ™ ™ ™ Column : YMC-Pack Pro C18 (5µm, 120Å) Column : YMC-Pack ODS-A (5µm, 120Å) 150 x 4.6 mm I.D. 150 x4.6 mm I.D. Part number : AS12S051546WT Part number : AA12S051546WT Eluent : methanol/water (92/8) Eluent : acetonitrile/methanol/THF (50/45/5) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 35 ˚C Temperature : 30 ˚C Detection : UV at 325 nm, 0.02 AUFS Detection : VIS at 450 nm, 0.16 AUFS Injection : 20 µL (0.5 µg/mL) Injection : 5 µL (0.05 mg/mL)

ß-Carotene ß-Carotene

1. ß - Carotene 1. ß - Carotene

(M910529A) (A970716K)

Column: : YMC-Pack™ CN (5 µm, 120Å) Column : YMC-Pack™ ODS-AL™ (5µm, 120Å) 250 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : CN12S052546WT Part number : AL12S051546WT Eluent: : acetonitrile/water/THF (35/35/30) Eluent : methanol/chloroform (96/4) Flow rate: : 1.0 mL/min Flow rate : 1.5 mL/min Temperature : 30 ˚C Temperature : 35 ˚C Detection : VIS at 450nm, 0.32 AUFS Detection : VIS at 455 nm, 0.04 AUFS Injection : 7 µL (0.125 mg/mL) Injection : 20 µL (4.0 µg/mL)

Vitamin D Vitamin D

1. Ergocalciferol (Vitamin D2)

1. Cholecalciferol (Vitamin D3)

(C970808E) (C970808H)

Column : YMC-Pack™ SIL (5µm, 120Å) Column : YMC-Pack™ SIL (5µm, 120Å) 250 x 4.6 mm I.D. 250 x4.6 mm I.D. Part number : SL12S052546WT Part number : SL12S052546WT Eluent : hexane/2-propanol (99.6/0.4) Eluent : hexane/2-propanol (99.6/0.4) Flow rate : 1.6 mL/min Flow rate : 1.6 mL/min Temperature : ambient (28 ˚C) Temperature : ambient (28 ˚C) Detection : UV at 265 nm, 0.01 AUFS Detection : UV at 265 nm, 0.01 AUFS Injection : 100 µL (1.0 µg/mL) Injection : 100 µL (1.0 µg/mL)

Tocopherols Tocopherols

1. α --Tocopherol 2. β -- Tocopherol 3. γ −Tocopherol 4. δ −Tocopherol

1. α -Tocopherol

2. β -Tocopherol

3. γ -Tocopherol

4. δ -Tocopherol

(G910523S) (S970702A)

Column : YMC-Pack™ SIL (5µm, 120Å) Column : YMC-Pack™ SIL (5µm, 120Å) 150x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : SL12S051546WT Part number : SL12S052546WT Eluent : hexane/THF/acetic acid (97/3/0.25) Eluent : hexane/2-propanol/acetic acid (1000/6/5) Flow rate : 1.0 mL/min Flow rate : 1.4 mL/min Temperature : 30 ˚C Temperature : 35 ˚C Detection : UV at 295 nm, 0.08 AUFS Detection : FLS at Ex 298 nm, Em 325 nm Injection : 10 µL (0.20 ~0.31 mg/mL) Injection : 20 µL (5~20µg/mL)

Tocopherols Tocopherols

1. α -Tocopherol 2. β -Tocopherol 3. γ -Tocopherol 1. α−Tocopherol 4. δ -Tocopherol

2. β−Tocopherol

3. γ−Tocopherol

4. δ− TocopherolText

(T910501A) (T920728E)

Column : YMC-Pack™ Polyamine II™ Column: : YMC-Pack™ Polyamine II™ 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : PB12S052546WT Part number : PB12S052546WT Eluent : n-hexane/tetrahydrofuran/acetic acid/water (93/7/0.4/0.01) Eluent : hexane/ethyl acetate (70/30) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 295 nm, 0.08 AUFS Detection : UV at 295 nm, 0.08 AUFS Injection : 7 µL (0.25 mg/mL) Injection : 7 µL (0.25 mg/mL)

Tocopherols Tocopherols

1. α−Tocopherol

2. β−Tocopherol 1. α−Tocopherol

3. γ−Tocopherol 2. β−Tocopherol

3. γ−Tocopherol 4. δ−Tocopherol

4. δ−Tocopherol

(S921217A) (S930726A) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 250 x 4.6 mm I.D. 250 x 4.6mm I.D. Part number : AA12S05246WT Part number : JM08S042546WT Eluent : 2-propanol/water (75/25) Eluent : 2-propanol/water (68/32) Flow rate : 0.3 mL/min Flow rate : 0.5 mL/min Temperature : 30 ˚C Temperature : 30˚C Detection : UV at 295 nm, 0.08 AUFS Detection : UV at 295nm, 0.032 AUFS Injection : 7 µL (0.25 mg/mL) Injection : 7 µL (0.25mg/mL) Ref.) Y.Satomura, M. Kimura and Y. Itokawa, J.Chromatogr., 625, 372-376 (1992)

Tocopherols Tocopherol and Tocopherol acetate

1. α-Tocopherol

1. α−Tocopherol 2. β-Tocopherol

3. γ-Tocopherol 2. α−Tocopherol acetate

4. δ-Tocopherol

(S921216C) (S970426E)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : AA12S051546WT Eluent : 2-propanol/water (65/35) Eluent : 2-propanol/water (65/35) Flow rate : 0.7 mL/min Flow rate : 0.7 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 295 nm, 0.04 AUFS Detection : UV at 295 nm, 0.04 AUFS Injection : 7 µL (0.25 mg/mL) Injection : 7 µL (0.25 mg/mL) Ref.) Y.Satomura, M. Kimura and Y. Itokawa, J.Chromatogr., 625, 372-376 (1992) Ref.) Y.Satomura, M. Kimura and Y. Itokawa, J.Chromatogr., 625, 372-376 (1992

Tocopherol and Tocopherol acetate Vitamin K

1. α−Tocopherol 1. Menaquinone (Vitamin K2)

2. Phylloquinone (Vitamin K ) 2. α−Tocopherol acetate 1

(A970728B) (S970426C) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : AA12S051546WT Eluent : methanol/ethanol (5/1) Eluent : methanol/water (98/2) Flow rate : 0.8 mL/min Flow rate : 1.0 mL/min Temperature : 40 ˚C Temperature : 30 ˚C Detection : FLS at Ex 320 nm, Em 430 nm Detection : UV at 284 nm, 0.51 AUFS reduction column (platinum black, 10 x 4.0 mmI.D.) Injection : 20 µL (1 mg/mL) Injection : 20 µL (100 ng/mL)

Water-soluble vitamins Water-soluble vitamins

1. L-Ascorbic acid (Vitamin C) 1. Nicotinic acid 2. Nicotinic acid 2. Pantothenic acid (Na salt) 3. Pyridoxine HCl (Vitamin B ) 3. Pyridoxine HCl (Vitamin B ) 6 6 4. Nicotinamide 4. Nicotinamide 5. Thiamine HCl (Vitamin B1) 5. Thiamine HCl (Vitamin B1) 6. Folic acid

7. Cyanocobalamin (Vitamin B12)

8. Riboflavin (Vitamin B2)

(G910415A)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) (G910523O) 150 x 4.6 mm I.D. Part number : AA12S051546WT Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Eluent : A)20mM KH2PO4 - K2HPO4 (pH 6.6) 150 x 4.6 mm I.D. B)20mM KH2PO4 - K2HPO4 (pH 6.6)/acetonitrile (80/20) Part number : AM12S051546WT 0%B (0-5min), 0-100%B (5-20min, linear), 100%B (20-25min) Eluent : 20mM NaH2PO4 - Na2HPO4 (pH 6.5) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.32 AUFS Detection : UV at 210 nm, 0.08 AUFS Injection : 10 µL (0.04~0.31 mg/mL) Injection : 5 µL (0.02~.58 mg/mL)

Water-soluble vitamins A drink including vitamins

1. L-Ascorbic acid (Vitamin C) 1. L-Ascorbic acid (Vitamin C)

2. Pyridoxine HCl (Vitamin B6) 2. Pyridoxine HCl (Vitamin B6) 3. Nicotinamide 3. Nicotinamide 4. Panthenol 4. Panthenol 5. Caffeine 5. Caffeine 6. Flavin mononucleotide 6. Flavin mononucleotide sodium salt

sodium salt (Vitamin B2) (Vitamin B2)

(F001004A) (F001004B) Column : Hydrosphere™ C18 (5µm, 120Å) Column : Hydrosphere™ C18 (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : HS12S051546WT Part number : HS12S051546WT Eluent : A)50mM KH2PO4-H3PO4(pH3.5) B)50mM KH2PO4-H3PO4(pH3.5)/acetonitrile (80/20) Eluent : A)50mM KH2PO4-H3PO4(pH3.5) 0-60%B(0-20min), 60%B(20-30min) B)50mM KH2PO4-H3PO4(pH3.5)/acetonitrile (80/20) 0-60%B(0-20min), 60%B(20-30min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 210 nm, 0.32AUFS Detection : UV at 210 nm, 0.32AUFS Injection : 5 µL Injection : 5 µL (0.05 ~ 0.40 mg/mL Sample : 5 times dilution with 5% metaphosphoric acid

Water-soluble vitamins A drink including vitamins 1. Thiamine HCl (Vitamin B ) 1. Thiamine HCl (Vitamin B ) 1 1 2. Pyridoxine HCl (Vitamin B ) 2. Pyridoxine HCl (Vitamin B ) 6 6 3. Nicotinamide 3. Nicotinamide 4. Caffeine 4. Caffeine 5. Flavin mononucleotide sodium salt 5. Flavin mononucleotide sodium salt (Vitamin B2) (Vitamin B2)

(F001011A) (F001011B)

Column : Hydrosphere™ C18 (5µm, 120Å) Column : Hydrosphere™ C18 (5µm, 120Å) 150 x4.6 mm I.D. 150 x4.6 mm I.D. Part number : HS12S051546WT Part number : HS12S051546WT

Eluent : A)50mM KH2PO4-H3PO4(pH3.5) Eluent : A)50mM KH2PO4-H3PO4(pH3.5)

B)50mM KH2PO4-H3PO4(pH3.5)/acetonitrile (80/20) B)50mM KH2PO4-H3PO4(pH3.5)/acetonitrile (80/20) 0-90%B(0-20min), 90%B(20-30min) 0-90%B(0-20min), 90%B(20-30min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.32AUFS Detection : UV at 254 nm, 0.32AUFS Injection : 15 µL Injection : 15 µL Sample : 5 times dilution with water Sample : 5 times dilution with water

Water-soluble vitamins Water-soluble vitamins

1. p-Aminobenzoic acid (PABA) 1. p-Aminobenzoic acid (PABA) 2. Biotin (Vitamin H) 2. Biotin (Vitamin H) 3. Riboflavin (Vitamin B ) 2 3. Cyanocobalamin (Vitamin B12) 4. Cyanocobalamin (Vitamin B ) 12 4. Riboflavin (Vitamin B2)

(M920212B) (M920212A)

Column : YMC-Pack™ Phenyl (5µm, 120Å) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : PH12S051546WT Part number : AM12S051546WT

Eluent : acetonitrile/50mM NH4H2PO4 (10/90) Eluent : acetonitrile/50mM NH4H2PO4 (10/90) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 210 nm, 0.16 AUFS Detection : UV at 210 nm, 0.16 AUFS Injection : 10 µL (0.02 ~ 0.30 mg/mL) Injection : 10 µL (0.02 ~ 0.30 mg/mL)

Water-soluble vitamins Water-soluble vitamins

(M920212C) (M920212D)

™ ™ ™ Column : YMC-Pack TMS (5µm, 120Å) Column : YMC-Pack C4 (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : TM12S051546WT Part number : BU12S051546WT

Water-soluble vitamins Water-soluble vitamins

1. L-Ascorbic acid (Vitamin C) 1. L-Ascorbic acid (Vitamin C) 2. Orotic acid (Vitamin B ) 2. Orotic acid (Vitamin B13) 13 3. Pyrroloquinoline quinone 3. Pyrroloquinoline quinone (PQQ)

(PQQ) 4. Pyridoxine HCl (Vitamin B6) 5. Nicotinamide 4. Pyridoxine HCl (Vitamin B6) 5. Nicotinamide

(M920207B) (M920207A)

Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Column : YMC-Pack™ TMS (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AM12S051546WT Part number : TM12S051546WT

Eluent : 100mM CH3COOH/100mM CH3COONH4 (30/70, pH 5.1) Eluent : 100mM CH3COOH/100mM CH3COONH4 (30/70, pH 5.1) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.16 AUFS Detection : UV at 254 nm, 0.16 AUFS Injection : 10 µL (0.04 ~ 0.20 mg/mL) Injection : 10 µL (0.04 ~ 0.20 mg/mL)

Water-soluble vitamins Water-soluble vitamins

1. Nicotinic acid 1. Nicotinic acid 2. Erythorbic acid 2. Erythorbic acid 3. L-Ascorbic acid 3. L-Ascorbic acid

(T920525A) (T920525B)

Column : YMC-Pack™ Polyamine II™ Column : YMC-Pack™ NH2 (5µm,120Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : PB12S052546WT Part number : NH12S052546WT

Eluent : acetonitrile/50mM NH4H2PO4 (70/30) Eluent : acetonitrile/50mM NH4H2PO4 (70/30) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 40 ˚C Temperature : 40 ˚C Detection : UV at 250 nm, 0.16 AUFS Detection : UV at 250 nm, 0.16 AUFS Injection : 10 µL (0.05 ~ 0.1 mg/mL) Injection : 10 µL (0.05 ~ 0.1 mg/mL)

Water-soluble vitamins Water-soluble vitamins

1. Pyridoxine HCl (vitamin B6) 2. Nicotinamide 3. Benfotiamine 4. Cyanocobalamin 5. Riboflavin 1. Xanthine(I.S.)

2. Pyridoxal HCl

3. Pyridoxine HCl

4. Nicotinamide

(S930712E) (S960912B)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : YMC-Pack™ Pro C18™ (5µm,120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : AS12S051546WT

Eluent : acetonitrile/50mM (NH4)2HPO4 (10/90) Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.13 AUFS Detection : UV at 254 nm, 0.13 AUFS Injection : 5 µL (0.04 ~ 0.48 mg/mL) Injection : 5 µL (0.05 ~ 0.2 mg/mL)

Water-soluble vitamins Allantoin and water-soluble vitamins

H2N COOH CONH2 1. p -Aminobenzoic acid 1 N 2 1.Nicotinamide COOH OH OH OH N CH CH

2 2 CH CH CH2OH 2.Nicotinic acid N H C N O 3 3 NH CHO H3C N O 3 4 2.Riboflavin HO CH2OH 4 5 1 CH3 ÅEHCl N H 3. Pyridoxal HCl H2NCONH N O O N H CH2OH 3.Allantoin HO CH2OH CH2OH CH3 ÅEHCl N HO CH2OH 4.Pyridoxine HCl CH3 N EÅHCl 4.Pyridoxine HCl CONH2 N 5.Nicotinamide

0 2 4 6 min 051015 20 min (S010125A) (S971121D)

Column : Hydrosphere™ C18 (3µm, 120Å) Column : YMC-Pack™ NH2 (5µm, 120Å) 50 x 2.0 mm I.D. 250 x 4.6 mm I.D. Part number : HS12S030502WT Part number : NH12S052546WT

Eluent : 10mM CH3COONH4 Eluent : 50mM NH4H2PO4 - H3PO4 (pH 2.5) / acetonitrile (20/80) Flow rate : 0.2 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.128 AUFS Detection : UV at 210 nm, 0.064 AUFS Injection : 2 µL (0.01 ~ 0.1 mg/mL) Injection : 8 µL (0.01 - 0.04 mg/mL)

Thiamine and its modified compounds Riboflavin and related compounds

1 OH OH OH O CH2CH CH CH CH2O P ONa

H3C N N O O 1. Thiamine HCl NH NH O P ONa 2 H3C N N O N O CH2 N N O

OH OH 2. Benfotiamine 2 Flavin adenine dinucleotide disodium 1 3

2 OH OH OH O CH2CH CH CH CH2O P OH

H3C N N O ONa NH H3C N 3. Dicethiamine HCl O Flavin sodium phosphate (Flavin mononucleotide sodium salt)

3 OH OH OH CH2CH CH CH CH2OH

N N O 4. Bisbentiamine H3C NH H3C N O Riboflavin

(S940202D) 0 10 20 min Column : J’Sphere™ ODS-H80 (4µm, 80Å) (S000803B) 75 x 4.6 mm I.D. Part number : JH08S04L546WT Column : Hydrosphere™ C18 (5µm, 120Å)

Eluent : A)50mM (NH4)2HPO4 150 x 4.6 mm I.D.

B)methanol/50mM (NH4)2HPO4 (60/40) Part number : HS12S051546WT 10-100%B (0-15min, linear), 100%B (15-30min) Eluent : 50mM KH2PO4 - K2HPO4 (pH 5.9) / acetonitrile (90/10) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.26 AUFS Detection : UV at 260 nm, 0.32 AUFS Injection : 10 µL (0.1~ 0.2 mg/mL) Injection : 10 µL (0.1 mg/mL)

Nicotinic acid and derivatives Pantothenyl alcohol

1. Nicotinamide adenine dinucleotide phosphate oxidized form (NADP) 2. Nicotinic acid 3. Nicotinamide adenine dinucleotide phosphate 1. Pantothenyl alcohol reduced form (NADPH) 4. Nicotinamide 5. β - Nicotinamide adenine dinucleotide (NAD) 6. β - Nicotinamide adenine dinucleotide reduced form 2. Resorcinol(I.S.) (NADH)

(F000707B) (S961119B)

Column : Hydrosphere™ C18 (5µm,120Å) Column : YMC-Pack™ Pro C18™ (5µm,120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : HS12S051546WT Part number : AS12S051546WT

Eluent : 20mM KH2PO4-K2HPO4(pH6.9)/acetonitrile (99/1) Eluent : acetonitrile/water (5/95) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.13 AUFS Detection : UV at 210 nm, 0.13 AUFS Injection : 6 µL (0.05 ~ 0.10 mg/mL) Injection : 5 µL (0.05 ~ 0.4 mg/mL)

Coenzyme A and derivatives L-Ascorbic acid and erythorbic acid

1. Malonyl-CoA

1. Erythorbic acid 2. Coenzyme A glutathione disulfide 2. L-Ascorbic acid (Vitamin C)

3. Coenzyme A

4. Methylmalonyl-CoA

5. Su ccinyl-CoA

6. 3-Hydroxy-3-methylglutaryl- CoA (HMG-CoA)

7. Acetoacetyl-CoA

8. Acetyl-CoA

9. Coenzyme A oxidized

10. Propionyl-CoA

Courtesy of Dr. Takamura, Ibaraki University (S940630E)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) (T910425A) 150 x 4.6 mm I.D. Part number : JM08S041546WT Column : YMC-Pack™ Polyamine II™

Eluent : A)methanol/50mM KH2PO4 (10/90) 250 x 4.6 mm I.D.

B)methanol/50mM KH2PO4 (30/70) Part number : PB12S052546WT 10%B (0-5min), 10-100%B (5-30min) Eleunt : acetonitrile/50mM NH4H2PO4 (70/30) Flow rate : 0.7 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 260 nm, 0.016 AUFS Detection : UV at 250 nm, 0.16 AUFS Injection : 10 µL (16.7 nmol/mL) Injection : 10 µL (0.1 mg/mL)

L-Ascorbic acid and erythorbic acid 2,4-Dinitrophenylhydrazone of L-ascorbic acid

1. L-Ascorbic acid (Vitamin C) 1. L-Ascorbic acid 2,4-DNPH 2. Erythorbic acid

(G910523N) (C970808K)

Column : YMC-Pack™ ODS-AM™ (5µm,120Å) Column : YMC-Pack™ SIL (5µm,120Å) 150 x 4.6 mm I.D. 150 x 6.0 mm I.D. Part number : AM12S051546WT Part number : SL12S051506WT

Eluent : 20mM CH3COONH4 in 5mM tetrabutylammonium bromide Eluent : hexane/ethyl acetate/2-propanol/acetic acid (40/30/2/1) Flow rate : 1.0 mL/min Flow rate : 1.5 mL/min Temperature : 30 ˚C Temperature : 35 ˚C Detection : UV at 250 nm, 0.08 AUFS Detection : VIS at 540 nm, 0.01 AUFS Injection : 5 µL (0.05 mg/mL) Injection : 20 µL (10 ~ g/mL)

Monosaccharides and disaccharides Monosaccharides and disaccharides

1. Fructose 1. Fructose 2. Glucose 2. Glucose 3. Sucrose 3. Sucrose 4. Maltose 4. Maltose 5. Lactose 5. Lactose

(T910726A) (T910723A)

Column : YMC-Pack™ Polyamine II™ Column : YMC-Pack™ Polyamine II™ 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : PB12S052546WT Part number : PB12S052546WT Eluent : acetonitrile/water (75/25) Eluent : ethanol/ethyl acetate/acetonitrile/water (30/40/5/25) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 26 ˚C Temperature : 26˚C Detection : RI, 32 x 10-6 RIU/FS Detection : RI, 32 x 10-6 RIU/FS Injection : 5 µL (14.3 ~ 28.6 mg/mL) Injection : 5 µL (14.3 ~ 28.6 mg/mL)

Monosaccharides and disaccharides Monosaccharides

1. Fructose 1. Rhamnose 2. Glucose 2. Fucose 3. Sucrose 3. Xylose 4. Maltose 4. Fructose 5. Lactose 5. Mannose 6. Glucose 7. Galactose

(T910718E) (T910718D)

Column : YMC-Pack™ Polyamine II™ Column : YMC-Pack™ Polyamine II™ 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. x 2 Part number : PB12S052546WT Part number : PB12S052546WT Eluent : ethanol/ethyl acetate/acetonitrile/water (30/40/5/25) Eluent : ethanol/ethyl acetate/acetonitrile/water (30/30/23/17) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 35 ˚C Temperature : 26 ˚C Detection : RI, 32 x 10-6 RIU/FS Detection : RI, 16 x 10-6 RIU/FS Injection : 5 µL (14.3 ~ 28.6 mg/mL) Injection : 25 µL (0.65 ~ 115.6 mg/mL)

Unsaturated chondro-disaccharides Sugar alcohols

1. ∆ Di-0S 1. Glycerol 2. ∆ Di-6S 2. Erythritol 3. ∆ Di-4S 3. Xylitol 4. ∆ Di-diSD 4. Ribitol 5. ∆ Di-diSE 5. Arabitol 6. ∆ Di-triS 6. Sorbitol 7. Mannitol 8. Maltitol 9. Inositol

(T910920A)

™ Column : YMC-Pack NH2 (5µm,120Å) (T910718B) 250 x 4.6 mm I.D. Part number : NH12S052546WT Column : YMC-Pack™ Polyamine II™ 250 4.6 mm I.D. Eluent : A) 0.01M NH4H2PO4 - (NH4)2HPO4(pH 6.7) Part number : PB12S052546WT B) 0.5M NH4H2PO4 - (NH4)2HPO4(pH 6.7) 0%B (0-5min), 0-100%B (5-30min,linear), 100%B (30-40min) Eluent : ethanol/ethyl acetate/acetonitrile/water (30/30/25/15) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 232 nm, 0.32 AUFS Detection : RI, 32 x 10-6 RIU/FS Injection : 9 µL (0.12 ~ 0.24 mg/mL) Injection : 15 µL (1.7 ~ 3.3 mg/mL)

Oligosaccharides Oligosaccharides

1. 2. 1. 2.

(A970625G) (A970701G)

™ ™ ™ Column : YMC-Pack Polyamine II Column : YMC-Pack NH2 (5µm, 120Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : PB12S052546WT Part number : NH12S052546WT Eluent : acetonitrile/water (70/30) Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (26˚C) Temperature : ambient (27 ˚C) Detection : RI, 32 x 10-6 RIU/FS Detection : RI, 32 x 10-6 RIU/FS Injection : 20 µL (4.0 mg/mL) Injection : 20 µL (4.0 mg/mL)

Fructooligosaccharides Oligosaccharides

1. Fruƒβ 2→1 α Glc (Sucrose) 1 2. Frufƒβ 2→ 1Fruƒβ2 → 1 α Glc 1. (1-Kestose) 2. 3. Frufƒβ 2→[1Fruf β2]2 → 1 α Glc (Nystose)

4. Frufƒβ 2→[1Fruf β2]3→1 α Glc 2 (1-Fructofuranosyl-D-nystose)

0 10 20 30 min

(F000725B) (A970627F)

Column : Hydrosphere™ C18 (5µm,120Å) Column : YMC-Pack™ Polyamine II™ 150 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : HS12S051546WT Part number : PB12S052546WT Eluent : water Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : ambient (26 ˚C) Detection : RI, 64 x 10-6 RIU/FS Detection : RI, 32 x 10-6 RIU/FS Injection : 5 µL (10 mg/mL) Injection : 20 µL (4.0 mg/mL)

Oligosaccharides of soft drink Oligosaccharides

1. 1. (Lactosyl fructoside) 2.

(A970630C) (A970628H)

Column : YMC-Pack™ Polyamine II™ ™ ™ 250 x 4.6 mm I.D. Column : YMC-Pack Polyamine II Part number : PB12S052546WT 250 x 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Part number : PB12S052546WT Flow rate : 1.0 mL/min Eluent : acetonitrile/water (70/30) Temperature : ambient (27 ˚C) Flow rate : 1.0 mL/min Detection : RI, 32 x 10-6 RIU/FS Temperature : ambient (26 ˚C) Injection : 10 µL Detection : RI, 32 x 10-6 RIU/FS Sample : 10 times dilution with water Injection : 20 µL (4.0 mg/mL)

Oligosaccharides Maltooligosaccharides

1. Maltoundecaose (G11)

2. Maltododecaose (G12)

3. Maltotridecaose (G13) 4. Maltotetradecaose (G ) 1. 14 5. Maltopentadecaose (G ) (Lactosyl fructoside) 15

(T910718A)

(A970703A) Column : YMC-Pack™ Polyamine II™ 250 x 4.6 mm I.D. ™ Column : YMC-Pack NH2 (5µm,120Å) Part number : PB12S052546WT 250 x 4.6 mm I.D. Eluent : acetonitrile/water (55/45) Part number : NH12S052546WT Flow rate : 1.0 mL/min Eluent : acetonitrile/water (70/30) Temperature : 26 ˚C Flow rate : 1.0 mL/min Detection : RI, 32 x 10-6 RIU/FS Temperature : ambient (27 ˚C) Injection : 200 µl (0.71 ~ 0.95 mg/mL) Detection : RI, 32 x 10-6 RIU/FS Sample : Manufactured by Nakano Vinegar Co., Ltd., Injection : 20 µL (4.0 mg/mL) Nakano Central Research Institute

Maltooligosaccharides Maltooligosaccharides

1. Glucose (G ) 1 1. Glucose(G1) 2. Maltose (G ) 2 2. Maltose(G2) 3. Maltotriose (G3) 3. Maltotriose (G3) 4. Maltotetraose (G4) 4. Maltotetraose (G4) 5. Maltopentaose (G5) 5. Maltopentaose (G5) 6. Maltohexaose (G6) 6. Maltohexaose (G6) 7. Maltoheptaose (G7) 7. Maltoheptaose (G7)

(F000612D) Courtesy of Dr.Kimura and Dr.Chiba, Hokkaido University, Japan (Y910501A) Column : Hydrosphere™ C18 (5µm,120Å) 250x 4.6 mm I.D. Column : YMC-Pack™ ODS-AQ™ (5µm,120Å) Part number : HS12S052546WT 250 x 10.0 mm I.D. Eluent : water Part number : AQ12S052510WT Flow rate : 1.0 mL/min Eluent : water Temperature : ambient (26˚C) Flow rate : 3.0 mL/min Detection : RI, 64 x 10-6 RIU/FS Temperature : ambient (25 ˚C) Injection : 10 µL (5.0 mg/mL) Detection : RI

Maltooligosaccharides Maltooligosaccharides

1. Maltoundecaose (G11) 1. Maltoundecaose (G11) 2. Maltododecaose (G ) 2. Maltododecaose (G12) 12 3. Maltotridecaose (G13) 3. Maltotridecaose (G13) 4. Maltotetradecaose (G14) 4. Maltotetradecaose (G14) 5. Maltopentadecaose (G15) 5. Maltopentadecaose (G15)

(M910604K) (M910604L)

Column : YMC-Pack™ ODS-A™ 5µm (300Å) Column: : YMC-Pack™ ODS-A™ (5µm, 120Å) 250 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA30S052546WT Part number : AA12S051546WT Eluent : water Eluent : water Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 20 ˚C Temperature : 20 ˚C Detection : RI, 8 x 10-6 RIU/FS Detection : RI, 8 x 10-6 RIU/FS Injection : 25 µl (1mg/mL) Injection : 25 µL (1 mg/mL) Sample : Manufactured by Nakano Vinegar Co., Ltd., Sample : Manufactured by Nakano Vinegar Co., Ltd., Nakano Central Research Institute Nakano Central Research Institute

Maltooligosaccharides Maltooligosaccharides

1. Maltoundecaose (G ) 1. Maltoundecaose (G11) 11 2. Maltododecaose (G ) 2. Maltododecaose (G12) 12 3. Maltotridecaose (G ) 3. Maltotridecaose (G13) 13 4. Maltotetradecaose (G ) 4. Maltotetradecaose (G14) 14 5. Maltopentadecaose (G ) 5. Maltopentadecaose (G15) 15

(M910604M) (M910604N)

Column: : YMC-Pack™ ODS-AQ™ (5µm, 120Å) Column : YMC-Pack™ ODS-A™ (5µm,120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AQ12S051546WT Part number : AA12S05I546WT Eluent : methanol/water (5/95) Eluent : methanol/water (5/95) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : RI, 8 x 10-6 RIU/FS Detection : RI, 8 x 10-6 RIU/FS Injection : 25 µL (1 mg/mL) Injection : 25 µL (1 mg/mL) Sample : Manufactured by Nakano Vinegar Co., Ltd., Sample : Manufactured by Nakano Vinegar Co., Ltd., Nakano Central Research Institute Nakano Central Research Institute

Maltooligosaccharides Maltooligosaccharides 1. Maltopentadecaose (G ) 15 1. Maltopentadecaose (G15) 2. Maltotridecaose (G ) 13 2. Maltotridecaose (G13) 3. Maltoundecaose (G ) 11 3. Maltoundecaose (G11) 4. Maltoheptaose (G ) 7 4. Maltoheptaose (G7 ) 5. Maltopentaose (G ) 5 5. Maltopentaose (G5 ) 6. Maltotriose (G ) 3 6. Maltotriose (G3 ) 7. Glucose (G ) 1 7. Glucose (G1 )

(G911216E) (G911216F)

Column : YMC-Pack™ Diol-60 Column : YMC™-Pack Diol-120 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL06S055008WT Part number : DL12S055008WT Eluent : water Eluent : water Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (24 ˚C) Detection : RI, 64 x 10-6 RIU/FS Detection : RI, 64 x 10-6 RIU/FS Injection : 60 µL (0.60 ~ 0.75 mg/mL) Injection : 60 µL (0.60 ~ 0.75 mg/mL)

Cyclodextrins Cyclodextrins

1. γ-Cyclodextrin 1. γ-Cyclodextrin 2. α-Cyclodextrin 2. α-Cyclodextrin 3. β-Cyclodextrin 3. β-Cyclodextrin

(M910417B) (M910417A)

Column : YMC™-Pack ODS-AQ™ (5µm,120Å) Column : YMC™-Pack ODS-A™ (5µm,120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AQ12S051546WT Part number : AA12S051546WT Eluent : methanol/water (7/93) Eluent : methanol/water (7/93) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : RI, 32 x 10-6 RIU/FS Detection : RI, 32 x 10-6 RIU/FS Injection : 8 µL (5~15 mg/mL) Injection : 8 µL (5~ 15 mg/mL)

Cyclodextrins Cyclodextrins

1. γ-Cyclodextrin 1. γ-Cyclodextrin 2. β-Cyclodextrin 2. α-Cyclodextrin 3. α-Cyclodextrin 3. β-Cyclodextrin 4. O-Dimethyl- β-cyclodextrin 5. O-Trimethyl-β-cyclodextrin

(M910423A)

Column : YMC-Pack™ ODS-A™ (5µm,120Å) 150 x 4.6 mm I.D. Part number : AA12S051546WT Eluent : A) water B) methanol 2%B (0-5min), 2-30%B (5-15min, linear), (G911216I) 30-100%B (15-20min, linear), 100%B (20-30min) Flow rate : 1.0 mL/min Temperature : 30 ˚C Column : YMC-Pack™ Diol-60 Detection :Evaporative Mass Analyzer (ACS Ltd.) 500x 8.0 mm I.D. Temp 150 Part number : DL06S055008WT Time Constant 5 Eluent : water PMT 3 Flow rate : 1.0 mL/min Attenuation 16 Temperature : ambient (24 ˚C) Pressure 20 psi Detection : RI, 32 x 10-6 RIU/FS Injection : 200 µL (1.5~15 mg/mL) Injection : 30 µL (1.67 mg/mL)

Cyclodextrins Pyridylamino(PA)-Sugar chains 1. PA-Sugar Chain 014 1. O-Dimethyl- β-cyclodextrin 2. O-Trimethyl-β-cyclodextrin

2. PA-Sugar Chain 001

3. PA-Sugar Chain 002

(G910607C) (M910417C) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AQ12S051546WT

Part number : AA12S051546WT Eluent : methanol/20mM NH4H2PO4 (5/95) Eluent : methanol/water (80/20) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : FLS at Ex.320 nm, Em. 400 nm, 8 mV/FS Detection : RI, 32 x 10-6 RIU/FS Injection : 2 µL (3.3 pmol/mL) Injection : 5 µL (15~20 mg/mL) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD.

Pyridylamino(PA)-Sugar chains Pyridylamino(PA)-Sugar chains 1. PA-Sugar Chain 014 1. PA-Sugar Chain 014

2. PA-Sugar Chain 001 2. PA-Sugar Chain 001

3. PA-Sugar Chain 002 3. PA-Sugar Chain 002

(G910607B) (G910607A)

Column : YMC-Pack™ ODS-A™ (5µm, 300Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA30S051546WT Part number : AA12S051546WT

Eluent : methanol/20mM NH4H2PO4 (5/95) Eluent : methanol/20mM NH4H2PO4 (5/95) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : FLS at Ex.320 nm, Em. 400 nm, 16 mV/FS Detection : FLS at Ex.320 nm, Em. 400 nm, 16 mV/FS Injection : 2 µL (3.3 pmol/mL) Injection : 2 µL (3.3 pmol/mL) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD. Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD.

Pyridylamino(PA)-Sugar chains Pyridylamino(PA)-Sugar chains

1. PA-Sugar Chain 014 1. PA-Sugar Chain 014

2. PA-Sugar Chain 001 2. PA-Sugar Chain 001

3. PA-Sugar Chain 002 3. PA-Sugar Chain 002

(G910620B) (G910620A)

™ ™ Column : YMC-Pack Polyamine II Column : YMC-Pack™ Polyamine II™ 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : PB12S051546WT Part number : PB12S051546WT Eluent : acetonitrile/20mM NH4H2PO4 (60/40) Eluent : methanol/20mM NH4H2PO4 (80/20) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : FLS at Ex. 320 nm, Em. 400 nm, 8 mV/FS Detection : FLS at Ex.320 nm, Em. 400 nm, 4 mV/FS Injection : 5 µL (3.3 pmol/mL) Injection : 3 µL (3.3 pmol/mL) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD. Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD.

Polydextrose Pullulans

1. Polydextrose 1. Pullulan P-800 (MW 853,000) 2. Pullulan P-50 (MW 48,000) 3. Pullulan P-20 (MW 23,700) 4. Pullulan P-5 (MW 10,700)

(G911216A) (G911226A) Column : YMC-Pack™ Diol-60 Column : YMC-Pack™ Diol-60 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL06S055008WT Part number : DL06S055008WT Eluent : water Eluent : water Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (25 ˚C) Detection : RI, 16 x 10-6 RIU/FS Detection : RI, 64 x 10-6 RIU/FS Injection : 180 µL (0.25 mg/mL) Injection : 40 µL (25 mg/mL) Sample : Shodex STANDARD P-82, manufactured by SHOWA DENKO K.K.

Pullulans Pullulans 1. Pullulan P-800 (MW 853,000) 1. Pullulan P-800 (MW 853,000) 2. Pullulan P-50 (MW 48,000) 2. Pullulan P-50 (MW 48,000) 3. Pullulan P-20 (MW 23,700) 3. Pullulan P-20 (MW 23,700) 4. Pullulan P-5 (MW 10,700) 4. Pullulan P-5 (MW 10,700)

(G911216B) (G911216C)

™ Column : YMC-Pack Diol-120 Column : YMC-Pack™ Diol-200 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL12S055008WT Part number : DL20S055008WT Eluent : water Eluent : water Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (24 ˚C) Detection : RI, 16 x 10-6 RIU/FS Detection : RI, 16 x 10-6 RIU/FS Injection : 180 µL (0.25 mg/mL) Injection : 180 µl (0.25 mg/mL) Sample : Shodex STANDARD P-82, manufactured by SHOWA DENKO K.K. Sample : Shodex STANDARD P-82, manufactured by SHOWA DENKO K.K.

Pullulans Chondroitin

1. Pullulan P-800 (MW 853,000) 1. Chondroitin, Na salt 2. Pullulan P-50 (MW 48,000) 3. Pullulan P-20 (MW 23,700) 4. Pullulan P-5 (MW 10,700)

(G911216D) (G920107A) Column : YMC-Pack™ Diol-300 500 x 8.0 mm I.D. Column : YMC-Pack™ Diol-300 Part number : DL30S055008WT 500 x 8.0 mm I.D. Eluent : water Part number : DL30S055008WT Flow rate : 1.0 mL/min Eluent : water Temperature : ambient (24 ˚C) Flow rate : 1.0 mL/min Detection : RI, 16 x 10-6 RIU/FS Temperature : ambient (24 ˚C) Injection : 180 µL (0.25 mg/mL) Detection : RI, 32 x 10-6 RIU/FS Sample : Shodex STANDARD P-82, manufactured by SHOWA DENKO K.K. Injection : 20 µL (5.0 mg/mL)

Chondroitin sulfate A Chondroitin sulfate B

1. Chondroitin sulfate A, Na salt 1. Chondroitin sulfate B, Na salt

(G920107C) (G920107E)

Column : YMC-Pack™ Diol-300 Column : YMC-Pack™ Diol-300 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL30S055008WT Part number : DL30S055008WT Eluent : water Eluent : water Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (24 ˚C) Detection : RI, 16 x 10-6 RIU/FS Detection : RI, 32 x 10-6 RIU/FS Injection : 20 µL (5.0 mg/mL) Injection : 20 µL (5.0 mg/mL)

Chondroitin sulfate C Hyaluronic acid

1. Chondroitin sulfate C, Na salt 1. Hyaluronic acid, Na salt

(G920107D) (G920107B)

Column : YMC-Pack™ Diol-300 Column : YMC-Pack™ Diol-300 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL30S055008WT Part number : DL30S055008WT Eluent : water Eluent : water Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (24 ˚C) Detection : RI, 16 x 10-6 RIU/FS Detection : RI, 8 x 10-6 RIU/FS Injection : 20 µL (5.0 mg/mL) Injection : 20 µL (5.0 mg/mL)

Heparin Heparan sulfate

1. Heparin, Na salt 1. Heparan sulfate, Na salt

(G920107I) (G920107F)

Column : YMC-Pack™ Diol-300 Column : YMC-Pack™ Diol-300 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL30S055008WT Part number : DL30S055008WT Eluent : water Eluent : water Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (24 ˚C) Detection : RI, 32 x 10-6 RIU/FS Detection : RI, 32 x 10-6 RIU/FS Injection : 20 µL (5.0 mg/mL) Injection : 20 µL (5.0 mg/mL)

Keratan sulfate Keratan polysulfate

1. Keratan sulfate, Na salt 1. Keratan polysulfate, Na salt

(G920107G) (G920107H)

Column : YMC-Pack™ Diol-300 Column : YMC-Pack™ Diol-300 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL30S055008WT Part number : DL30S055008WT Eluent : water Eluent : water Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (24 ˚C) Detection : RI, 32 x 10-6 RIU/FS Detection : RI, 32 x 10-6 RIU/FS Injection : 20 µL (5.0 mg/mL) Injection : 20 µL (5.0 mg/mL)

Nucleosides and bases Nucleosides and bases

1. Cytocine 1. Cytocine 2. Cytidine 2. Cytidine 3. Uracil 3. Uracil 4. Deoxycytidine 4. Deoxycytidine 5. Guanine 5. Guanine 6. Adenine 6. Adenine 7. Hypoxanthine 7. Hypoxanthine 8. Uridine 8. Uridine 9. Xanthine 9. Xanthine 10. Thymine 10. Thymine 11. Nosine 11. Inosine 12. Guanosine 12. Guanosine 13. Deoxyinosine 13. Deoxyinosine 14. Deoxyguanosine 14. Deoxyguanosine 15. Xanthosine 15. Xanthosine 16. Thymidine 16. Thymidine 17. Adenosine 17. Adenosine 18. Deoxyadenosine 18. Deoxyadenosine

(T910404C) (S921217A)

Column : YMC-Pack™ ODS-A™ (5µm,120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : AA12S052546WT Part number : AQ12S052546WT

Eluent : A)20mM CH3COOH - CH3COONH4 (pH 3.5) Eluent : A)20mM CH3COOH - CH3COONH4 (pH 3.5)

B)20mM CH3COOH - CH3COONH4 (pH 3.5)/methanol (90/10) B)20mM CH3COOH - CH3COONH4 (pH 3.5)/methanol (90/10) 30%B (0-5min), 30-100%B (5-13min,linear), 100%B (13-40min) 30%B (0-5min), 30-100%B (5-13min,linear), 100%B (13-40min) Flow rate : 0.7 mL/min Flow rate : 0.7 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 260 nm, 0.08 AUFS Detection : UV at 260 nm, 0.08 AUFS Injection : 12 µL (0.001 ~ 0.05 mg/mL) Injection : 12 µL (0.001 ~ 0.05 mg/mL)

Nucleic acid bases Nucleic acid bases 1. 5. 5 NH2 O N 3 N HN O N H2N N NH H Cytosine Guanine

2. 6. NH2 O CH N F HN 3 O N O N H 4 H 2 5-Fluorocytosine Thymine

1 3. 7. O NH2 N HN N O N N NH H Uracil Adenine 7 6 4. O HN F O N H 5-Fluorouracil

(S991029A) 0 5 10 min (J010209E)

Column : Hydrosphere™ C18 (3µm,120Å) Column : Hydrosphere™ C18 (5µm,120Å) 100 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : HS12S031046WT Part number : HS12S051546WT Eluent : 20mM KH PO - K HPO (pH 6.9) Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) 2 4 2 4 Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.128 AUFS Detection : UV at 254 nm, 0.064 AUFS Injection : 10 µL (0.01 - 0.02 mg/mL) Injection : 8 µL (0.01 - 0.02 mg/mL)

Orotic acid Nucleotides

(A000711A) (A000802B)

™ Column : Hydrosphere C18 (5µm,120Å) Column : Hydrosphere™ C18 (5µm,120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : HS12S051546WT Part number : HS12S051546WT Eluent : 20mM H PO 3 4 Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.5) / methanol (92/8) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.256 AUFS Detection : UV at 260 nm, 0.064 AUFS Injection : 10 µL (0.015 - 0.1 mg/mL) Injection : 15 µL (5 - 15 µg/mL)

Nucleotides Nucleotides

1. 5'-CMP 1. 5'-UTP 2. 5'-UMP 2. 5'-UDP 3. 5'-dCMP 5 3. 5'-CMP 4. 5'-CDP 4. 5'-UMP 5. 5'-GMP 1 6. 5'-UDP 5. 5'-dCMP 7. 5'-IMP 6. 5'-GMP 8. 5'-CTP 7 7. 5'-ATP 9. 5'-UTP 8. 5'-IMP 10. 5'-GDP 2 9. 5'-ADP 11. 5'-TMP 4 8 10. 5'-TMP 12. 5'-AMP 10 11. 5'-AMP 13. 5'-dGMP 3 6 11 14. 5'-GTP 9 15. 5'-TDP 16. 5'-ADP 17. 5'-TTP 18. 5'-dAMP 19. 5'-ATP

0 5 10 15 20 min (T910424A)

Column : YMC-Pack™ ODS-AM™ (5µm,120Å) 250 x 4.6 mm I.D. (A000922A) Part number : AM12S052546WT ™ Eluent : A) 0.2M TEAA* (pH6.6) Column : Hydrosphere C18 (5µm, 120Å) B) 0.2M TEAA (pH6.6)/acetonitrile (95/5) 150 x 4.6 mm I.D. 4%B (0-10min), 4-100%B (10-35min, linear), 100%B (35-50min) Part number : HS12S051546WT Eluent : 100mM KH PO - K HPO (pH 5.5) Flow rate : 1.0 mL/min 2 4 2 4 Temperature : 30 ˚C Flow rate : 1.0 mL/min Detection : UV at 260 nm, 0.16 AUFS Temperature : 30 ˚C Injection : 14 µL (0.029 ~ 0.16 mg/mL) Detection : UV at 260 nm, 0.256 AUFS * TEAA:triethylamine-acetic acid Injection : 10 µL (0.05 - 0.15 mg/mL)

Nucleotides Nucleotides

1. 5'-CMP 1. 5'-CMP 2. 5'-UMP 2. 5'-AMP 3. 5'-GMP 3. 5'-UMP 4. 5'-IMP 4. 5'-IMP 5. 5'-TMP 5. 5'-GMP 6. 5'-AMP

(A000912B) (T920525C) Column : Hydrosphere™ C18 (5µm, 120Å) Column : YMC-Pack™ Polyamine II™ 150 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : HS12S051546WT Part number : PB12S052546WT Eluent : 100mM KH PO 2 4 Eluent : 50mM KH PO - H PO (pH 3.5) Flow rate : 1.0 mL/min 2 4 3 4 Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 40 ˚C Detection : UV at 260 nm, 0.256 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 10 µL (0.1 - 0.3 mg/mL) Injection : 10 µL (0.05 ~ 0.1 mg/mL)

Nucleotides Oligonucleotides

1. 5'-CMP 1. c-Ha-ras/12 (20mer) 2. 5'-AMP GTGGGCGCC-AGC-GGTGTGGG 3. 5'-UMP Ser 2. c-Ha-ras/12 (20mer) 4. 5'-IMP GTGGGCGCC-GCC-GGTGTGGG 5. 5'-GMP Ala 3. c-Ha-ras/12 (20mer) GTGGGCGCC-GAC-GGTGTGGG Asp 4. c-Ha-ras/12 (20mer) GTGGGCGCC-GTC-GGTGTGGG Val 5. c-Ha-ras/12 (20mer) GTGGGCGCC-CGC-GGTGTGGG Arg

(G910620C)

Column : YMC-Pack™ ODS-A™ (5µm, 300Å) (T920525D) 150 x 4.6 mm I.D. Part number : AA30S051546WT Column : YMC-Pack™ NH2 (5µm, 120Å) Eluent : A)100mM triethylamine - acetic acid (pH 6.5)/acetonitrile (94/6) 250 x 4.6 mm I.D. B)100mM triethylamine - acetic acid (pH 6.5)/acetonitrile (85/15) Part number : NH12S052546WT 0-100%B (0-25min,linear) Flow rate : 1.0 mL/min Eluent : 50mM KH2PO4 - H3PO4 (pH 3.5) Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 40 ˚C Detection : UV at 260 nm,0.01 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 15 µL (2.0 pmol/µL ) Injection : 10 µL (0.05 ~ 0.1 mg/mL) Sample : TAKARA ras Gene Probe set, manufacutured by TAKARA SHUZO CO., LTD.

Poly(A) Sugar nucleotides

1. CDP-D-glucose 2. UDP-D-glucose 3 UDP-N-acetyl-D-glucosamine 4. GDP-D-mannose 5. ADP-D-glucose

Courtesy of Dr.Y.Baba, Kobe Women's College of Pharmacy (Y920608A)

Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) (T911206D) 150 x 6.0 mm I.D. Part number : AM12S051506WT Column : YMC-Pack™ Phenyl (5µm, 120Å) Eluent : A)0.1M phosophate buffer (pH 7) 250 x 4.6 mm I.D. B)0.1M phosophate buffer (pH 7) / acetonitrile (75/25) Part number : PH12S052546WT 20-27%B (0-15min), 27-35%B (15-90min) Eluent : 100mM TEAA1) (pH 6.0) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient Temperature : 37 ˚C Detection : UV at 260 nm Detection : UV at 260 nm, 0.16 AUFS Injection : 10 µL Injection : 5 µL (0.27 ~ 0.71 mg/mL) Sample : Poly(A) 1) TEAA: triethylamine-acetic acid

Sugar nucleotides Sugar nucleotides

1. CDP-D-glucose 1. CDP-D-glucose 2. UDP-D-glucose 2. UDP-D-glucose 3 UDP-N-acetyl-D-glucosamine 3 UDP-N-acetyl-D-glucosamine 4. GDP-D-mannose 4. GDP-D-mannose 5. ADP-D-glucose 5. ADP-D-glucose

(T911206B) (T911206E)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ 0DS-A™ (5µm, 300Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : AA12S052546WT Part number : AA30S052546WT Eluent : 20mM TEAA1) (pH 5.7)/acetonitrile (99/1) Eluent : 100mM TEAA1) (pH 6.0) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 5 µL (0.27~ 0.71 mg/mL) Injection : 5 µL (0.27~0.71 mg/mL) 1) TEAA: triethylamine-acetic acid 1) TEAA: triethylamine-acetic acid

Plasmid pBR322 restriction fragments

1. Plasmid pBR322 cleaved with restriction endonuclease Msp

(G920109B)

Column : YMC-Pack™ Diol-300 + Diol-200 500 x 8.0 mm I.D. x 2 Part number : DL30S055008WT , DL20S055008WT (x2)

Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Temperature : ambient (26 ˚C) Detection : UV at 260 nm, 0.01 AUFS Injection : 3 µL (0.49 mg/mL) Sample : Plasmid pBR322 cleaved with restriction endonuclease Msp I

Amino acids Taurine in nutritious drink

1. L-Valine 1. Taurine 2. L-Tyrosine 3. L-Phenylalanine 4. L-Tryptophan

(F001115C)

(A000419B) Column : Hydrosphere™ C18 (5µm, 120Å) 150 x4.6 mm I.D. Column : Hydrosphere™ C18 (5µm, 120Å) Part number : HS12S051546WT

150 x 4.6 mm I.D. Eluent : 10mM KH2PO4-K2HPO4(pH6.8)/acetonitrile (85/15) Part number : HS12S051546WT Flow rate : 1.0 mL/min

Eluent : 20mM KH2PO4 Temperature : 30 ˚C Flow rate : 1.0 mL/min Detection : (A)FLS at Ex 345 nm, Em 450 nm Temperature : 37 ˚C (B)UV at 254 nm, 0.32 AUFS Detection : UV at 210 nm, 0.16 AUFS Injection : 15 µL Injection : 5 µL (0.05 - 1.6 mg/mL) Sample : OPA derivative

PTC-amino acids PTH-amino acids

1. L-Aspartic acid 1. Asp 2. L-Glutamic acid 2. His 3. L-Serine 3. Arg 4. Glycine 4. Asn 5. L-Histidine 5. Glu 6. L-Arginine 6. Ser 7. L-Threonine 7. Gln 8. L-Alanine 8. Thr 9. L-Proline 9. Gly 10. NH3 10. Ala 11. L-Tyrosine 11. Tyr 12. L-Valine 12. É¢-Thr 13. L-Methionine 13. Pro 14. L-Cystine 14. Met 15. L-Isoleucine 15. Val 16. L-Leucine 16. Trp 17. L-Phenylalanine 17. Phe 18. L-Lysine 18. Lys 19. Ile 20. Leu

(G910523A) (K940331A)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 250 x 2.0 mm I.D. 250 x 4.6 mm I.D. Part number : JH08S042502WT Part number : AM12S052546WT Eluent : A)acetonitrile/THF/300mM TEAA* (pH 5.1) (10/4/90) Eluent : A) 60mM CH COONa - CH COOH (pH 6.6)/acetonitrile (94/6) 3 3 B)acetonitrile/THF/300mM TEAA (pH 5.1) (75/4/25) B) 60mM CH COONa - CH COOH (pH 6.6)/acetonitrile (40/60) 3 3 0-30%B (0-15min), 30-80%B (15-30min) 0-55%B (0-20min, linear), 100%B (20-25min), 0%B (25-35min) Flow rate : 0.2 mL/min Flow rate : 1.0 mL/min Temperature : 50 ˚C Temperature : 37 ˚C Detection : UV at 270 nm, 0.08 AUFS Detection : UV at 250 nm, 0.08 AUFS Injection : 30 µL (25pmol/10µL) Injection : 5 µL (62.5 pmoL/µL ) *TEAA: triethylamine-acetic acid Sample : PTC derivatives

1. L-Aspartic acid NBD-amino acids 2. L-Glutamic acid Peptides and proteins 3. L-Asparagine 4. L-Serine 1. Met-Enkephalin 5. L-Histidine 2. Leu-Enkephalin 6. Glycine 3. BSA 7. L-Arginine 4. α-Mating factor 8. L-Threonine 5. Insulin 9. Taurine 6. Ovalbumin 10. L-Alanine 11. L-Proline 12. L-Valine 13. L-Cystine 14. L-Methionine 15. L-Isoleucine 16. L-Leucine 17. L-Phenylalanine 18. L-Lysine 19. L-Tyrosine

(F001129E)

Column : Hydrosphere™ C18 (5µm, 120Å) 150 x 4.6 mm I.D. Part number : HS12S5051546WT (S921217A)

Eluent : A)20mM KH PO -K HPO (pH6.5) ™ ™ 2 4 2 4 Column : YMC-Pack PolymerC18 (6µm) B)20mM KH PO4-K HPO (pH6.5)/acetonitrile (50/50) 2 2 4 150 x 4.6 mm I.D. 15-25%B(0-25min), 25-40%B(25-30min), Part number : PC99S061546WT 40-65%B(30-50min),65-100%B(50-55min), Eluent : A)acetonitrile/water/TFA (20/80/0.05) 100%B(55-60min) B)acetonitrile/water/TFA (45/55/0.05) Flow rate : 1.0 mL/min(0-25min), 1.5 mL/min(25-60min) 0-100%B (0-30min,linear) Temperature : 30 ˚C Flow rate : 1.0 mL/min Detection : FLS at Ex 470 nm, Em 530 nm Temperature : 30 ˚C Injection : 20 µL Detection : UV at 220 nm, 0.32 AUFS Sample : NBD derivatives Injection : 30 µL

Glutathione, oxidized and reduced form Angiotensins

1. Glutathione (GSH) 1. Angiotensin III 2. Glutathione disulfide (GSSG) 2. Angiotensin II 3. [Asn1,Val5] Angiotensin áT 4. [Val5] Angiotensin I 5. Angiotensin I

(H900710D) (G910520A) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 4.6 mm I.D. Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Part number : AA12S051546WT 150 x 4.6 mm I.D. Eluent : 0.1M sodium perchlorate in A Part number : AM12S051546WT A)acetonitrile/water/TFA (5/95/0.05) Eluent : acetonitrile/20mM KH2PO4 (20/80) Flow rate : 0.5 mL/min Flow rate : 0.5 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 220 nm,0.64 AUFS Detection : UV at 220 nm,0.08 AUFS Injection : 10 µL Injection : 8 µL (0.05 ~ 0.15 mg/mL)

Angiotensins Insulins

1. Angiotensin III 1. Insulin (Bovine) 2. Angiotensin II 2. Insulin (Sheep) 3. Angiotensin I 3. Insulin (Human) 4. Insulin (Equine) 5. Insulin (Porcine)

(G910522C)

(H900723B) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 250 x 4.6 mm I.D. Column : YMC-Pack™ PolymerC18™ (6µm) Part number : AM12S052546WT 250 x 4.6 mm I.D. Eluent : A)acetonitrile/water/TFA (30/70/0.1) Part number : PC99S062546WT B)acetonitrile/water/TFA (32/68/0.1) Eluent : acetonitrile/water/TFA (18/82/0.05) 0%B (0-3min), 0-100%B (3-25min,linear), 100%B (25-40min) Flow rate : 0.5 mL/min Flow rate : 0.6 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 220 nm,0.08 AUFS Detection : UV at 220 nm, 0.16 AUFS Injection : 10 µL Injection : 15 µL (0.2 mg/mL)

Insulins Insulins

1. Insulin (Bovine) 1. Insulin (Bovine) 2. Insulin (Sheep) 2. Insulin (Sheep) 3. Insulin (Human) 3. Insulin (Human) 4. Insulin (Equine) 4. Insulin (Equine) 5. Insulin (Porcine) 5. Insulin (Porcine)

(G910522B) (G910522A)

Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 150 x 6.0 mm I.D. 250 x 6.0 mm I.D. Part number : AM12S051506WT Part number : AM12S052506WT Eluent : A)acetonitrile/water/TFA (30/70/0.1) Eluent : A)acetonitrile/water/TFA (30/70/0.1) B)acetonitrile/water/TFA (32/68/0.1) B)acetonitrile/water/TFA (32/68/0.1) 0%B (0-3min), 0-100%B (3-25min,linear), 100%B (25-40min) 0%B (0-3min), 0-100%B (3-25min,linear), 100%B (25-40min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 220 nm,0.16 AUFS Detection : UV at 220 nm,0.16 AUFS Injection : 15 µL (0.2 mg/mL) Injection : 15 µL (0.2 mg/mL)

Insulins Peptides

1. Insulin (Bovine) 1. Bradykinin 2. Insulin (Sheep) 2. Dynorphin A (Human,1-13) 3. Insulin (Human) 3. Eledoisin related peptide 4. Insulin (Porcine) 4. Neurotensin 5. Bombesin 6. Substance P

(G910522D) (A980827D)

Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Column : YMC-Pack™ Pro C18™ (3µm, 120Å) 150 x 6.0 mm I.D. 75 x 4.6 mm I.D. Part number : AM12S051506WT Part number : AS12S03L546WT Eluent : A)acetonitrile/0.01N HCl (28/72) Eluent : A) water / TFA (100/0.1) B)acetonitrile/0.01N HCl (35/65) B) acetonitrile / TFA (100/0.1) 0-100%B (0-60min,linear) 20-40%B (0-20min, linear) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 220 nm,0.16 AUFS Detection : UV at 220 nm, 0.32 AUFS Injection : 15 µL (0.25 mg/mL) Injection : 15 µL (0.05 - 0.15 mg/mL)

Peptides Peptides

1. Oxytocin 1. BAM-12P 2. Met-Enkephalin 2. [D-Ala2,Met5] 3. Leu-Enkephalin Enkephalinamide 4. Angiotensin I 3. α-Endorphin 5. α−Mating factor 4. Met-Enkephalin 6. Insulin 5. [D-Ala2,Met5]-Enkephalin 6. γ-Endorphin 7. β-Endorphin(Human) 1 4 6 5 23 7

051015 20 min

(A980827C) (A000308B)

Column : YMC-Pack™ Pro C18™ (3µm, 120Å) Column : Hydrosphere™ C18 (5µm, 120Å) 75 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S03L546WT Part number : HS12S051546WT Eluent : A) water / TFA (100/0.1) Eluent : A) water/TFA (100/0.1) B) acetonitrile / TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 20-40%B (0-20min, linear) 20-40%B(0-15 min, linear),40%B(15-20 min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 220 nm, 0.32 AUFS Detection : UV at 220 nm, 0.256 AUFS Injection : 15 µL (0.04 - 0.15 mg/mL) Injection : 10 µL (0.05 - 0.1 mg/mL)

Peptides Peptides

1. Met-Enkephalin 1. Bradykinin 2. Bradykinin 2. Met-Enkephalin 3. Angiotensin II 3. Angiotensin III 4. Leu-Enkephalin 4. Leu-Enkephalin 5. CCK-Octapeptide 6. Insulin Chain B

(S930909D)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) (O940804D) 150 x 4.6 mm I.D. Part number : JH08S041546WT Column : J’Sphere™ ODS-H80 (4µm, 80Å) Eluent : A)acetonitrile/water/TFA (5/95/0.1) 150 x 4.6 mm I.D. B)acetonitrile/water/TFA (40/60/0.1) Part number : JH08S041546WT 20-100%B (0-40min, linear) Eluent : acetonitrile/water/TFA (20/80/0.1) Flow rate : 1.5 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 220 nm, 0.26 AUFS Detection : UV at 220 nm, 0.32 AUFS Injection : 40 µL (0.067~0.13 mg/mL) Injection : 20 µL (34 ~ 65 µg/mL)

Peptides Peptides

1. Bradykinin 1. Bradykinin 2. Met-Enkephalin 2. Met-Enkephalin 3. Angiotensin III 3. Angiotensin III 4. Leu-Enkephalin 4. Leu-Enkephalin

(O940804A) (O940804B)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 2.0 mm I.D. 150 x 1.0 mm I.D. Part number : JH08S041502WT Part number : JH08S041501PFK Eluent : acetonitrile/water/TFA (20/80/0.1) Eluent : acetonitrile/water/TFA (20/80/0.1) Flow rate : 0.2 mL/min Flow rate : 0.1 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 220 nm, 0.08 AUFS Detection : UV at 220 nm, 0.08 AUFS Injection : 4 µL (34~65 µg/mL) Injection : 2 µL (34 ~ 65 µg/mL)

Peptides Human immunoglobulins

1. Bradykinin 1. Human IgM (κ),myeloma protein 2. Met-Enkephalin 2. Normal human IgA 3. Angiotensin III 3. Normal human IgG 4. Leu-Enkephalin

(O940804C) (G920108G)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : YMC-Pack™ Diol-300 + Diol-200 150 x 1.0 mm I.D. 500 x 8.0 mm I.D. x 2 Part number : JH08S041501PFK Part number : DL30S055008WT, DL20S055008WT (x2)

Eluent : acetonitrile/water/TFA (20/80/0.1) Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 0.05 mL/min Flow rate : 0.7 mL/min Temperature : 37 ˚C Temperature : ambient (24 ˚C) Detection : UV at 220 nm, 0.08 AUFS Detection : UV at 280 nm, 0.04 AUFS Injection : 1 µL (34 ~ 65 É g/mL) Injection : 60 µL (0.33 mg/mL)

Human immunoglobulins Human immunoglobulins

1. Human IgM (É»),myeloma protein 1. Human IgM (É»),myeloma protein 2. Normal human IgG 2. Normal human IgA

(G920108F) (G920108E)

Column : YMC-Pack™ Diol-300 Column : YMC-Pack™ Diol-300 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL30S055008WT Part number : DL30S055008WT Eluent : 0.1M NaH PO - Na HPO (pH 6.8) containing 0.1M Na SO 2 4 2 4 2 4 Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (24 ˚C) Detection : UV at 280 nm, 0.04 AUFS Detection : UV at 280 nm, 0.04 AUFS Injection : 40 µL (0.5 mg/mL) Injection : 40 µL (0.5 mg/mL)

Human IgM(κ),myeloma protein Normal human IgA

1. Normal human IgA 1. Normal human IgM (κ),myeloma protein

(G920108D) (G920108C)

Column : YMC-Pack™ Diol-300 Column : YMC-Pack™ Diol-300 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL30S055008WT Part number : DL30S055008WT

Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (24 ˚C) Detection : UV at 280 nm, 0.04 AUFS Detection : UV at 280 nm, 0.02 AUFS Injection : 20 µL (1.0 mg/mL) Injection : 20 µL (1.0 mg/mL)

Human IgG(λ),myeloma protein Normal human IgG

1. Normal human IgG 1. Human IgG (λ),myeloma protein

(G920108B) (G920108A)

Column : YMC-Pack™ Diol-300 Column : YMC-Pack™ Diol-300 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL30S055008WT Part number : DL30S055008WT

Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (24 ˚C) Detection : UV at 280 nm, 0.04 AUFS Detection : UV at 280 nm, 0.04 AUFS Injection : 20 µL (1.0 mg/mL) Injection : 20 µL (1.0 mg/mL)

Cytochrome c Peptide fragments from myoglobin 1. Cytochrome c (hexamer) 2. Cytochrome c (tetramer) 3. Cytochrome c (trimer) 1. 1-153 (MW 17,000) 4. Cytochrome c (dimer) 2. 1-131 (MW 14,500) 5. Cytochrome c (monomer) 3. 56-153 (MW 10,700) 4. 56-131 (MW 8,200) 5. 1-55 (MW 6,300) 6. 132-153 (MW 2,500)

(G911218A)

Column : YMC-Pack™ Diol-120 (G911217D) 500 x 8.0 mm I.D. Part number : DL12S055008WT Column : YMC-Pack™ Diol-200 + Diol-120 Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (70/30) 500 x 8.0 mm I.D. x 2 Flow rate : 0.7 mL/min Part number : DL20S055008WT, DL125055008WT (x2) Temperature : ambient (25 ˚C) Eluent : 0.1M KH PO - K HPO (pH 7.0) containing 0.2M NaCl 2 4 2 4 Detection : UV at 215 nm, 0.32 AUFS Flow rate : 1.0 mL/min Injection : 20 µL (2.0 mg/mL) Temperature : ambient (24 ˚C) Sample : cyanogen bromide cleavages of horse heart myoglobim. Detection : UV at 215 nm, 0.08 AUFS Molecular Weight Marker for proteins,manufactured by Injection : 30 µL (0.2 mg/mL) Fluka Chemie AG.

Peptides Peptides

1. Insulin (Bovine) 1. Insulin (Bovine) 2. Neurotensin 2. Neurotensin 3. Angiotensin II 3. Angiotensin II 4. Glycine 4. Glycine

(G911217A) (G911217B)

Column : YMC-Pack™ Diol-60 Column : YMC-Pack™ Diol-120 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. x 2 Part number : DL06S055008WT Part number : DL12S055008WT

Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (70/30) Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (70/30) Flow rate : 0.7 mL/min Flow rate : 0.7 mL/min Temperature : ambient (25 ˚C) Temperature : ambient (25 ˚C) Detection : UV at 215 nm, 0.16 AUFS Detection : UV at 215 nm, 0.16 AUFS Injection : 25 µL (0.07~5.3 mg/mL) Injection : 25 µL (0.07~5.3 mg/mL)

Peptides Glycine oligomers

1. Insulin (Bovine) 1. Pentaglycine 2. Neurotensin 2. Triglycine 3. Angiotensin II 3. Glycine 4. Glycine

(G911217C) (G911227B)

Column : YMC-Pack™ Diol-120 + Diol-60 Column : YMC-Pack™ Diol-60 500 x 8.0 mm I.D. x 2 500 x 8.0 mm I.D. Part number : DL12S055008WT, DL06S055008WT (x2) Part number : DL06S055008WT Eluent : 0.1M KH PO - K HPO4 (pH 7.0) 2 4 2 Eluent : 0.1M KH PO - K HPO (pH 7.0)/acetonitrile (70/30) containing 0.2M NaCl/acetonitrile (70/30) 2 4 2 4 Flow rate : 1.0 mL/min Flow rate : 0.7 mL/min Temperature : ambient (24 ˚C) Temperature : ambient (25 ˚C) Detection : UV at 215 nm, 0.08 AUFS Detection : UV at 215 nm, 0.08 AUFS Injection : 20 µL (0.25 ~ 2.5 mg/mL) Injection : 25 µL (0.07~5.3 mg/mL)

Casein Proteins for molecular weight marker

1. Casein

1. Glutamate dehydrogenase (MW 290,000) 2. Lactate dehydrogenase (MW 142,000) 3. Enolase (MW 67,000) 4. Adenylate kinase (MW 32,000) 5. Cytochrome c (MW 12,400)

(G920123B) (G911213A)

Column : YMC-Pack™ Diol-300 Column : YMC-Pack™ Diol-300 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. Part number : DL30S055008WT Part number : DL30S055008WT Eluent : 0.1M KH PO - K HPO (pH 7.0) containing 0.2M NaCl Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) 2 4 2 4 containing 0.2M NaCl/acetonitrile (90/10) Flow rate : 0.7 mL/min Flow rate : 1.0 mL/min Temperature : ambient (26 ˚C) Temperature : ambient (22 ˚C) Detection : UV at 280 nm, 0.08 AUFS Detection : UV at 215 nm, 0.16 AUFS Injection : 15 µL (100 µL/1 vial) Injection : 15 µL (1.0 mg/mL) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD.

Proteins for molecular weight marker Proteins for molecular weight marker

1. Glutamate dehydrogenase (MW 290,000) 2. Lactate dehydrogenase (MW 142,000) 1. Glutamate dehydrogenase (MW 290,000) 3. Enolase (MW 67,000) 2. Lactate dehydrogenase (MW 142,000) 4. Adenylate kinase (MW 32,000) 3. Enolase (MW 67,000) 5. Cytochrome c (MW 12,400) 4. Adenylate kinase (MW 32,000) 5. Cytochrome c (MW 12,400)

(G911214A) (S921217A)

Column : YMC-Pack™ Diol-200 Column : YMC-Pack™ Diol-200 500 x 8.0 mm I.D. 300 x 8.0 mm I.D. Part number : DL20S055008WT Part number : DL20S053008WT Eluent : 0.1M KH PO - K HPO (pH 7.0) containing 0.2M NaCl Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl 2 4 2 4 Flow rate : 0.7 mL/min Flow rate : 0.7 mL/min Temperature : ambient (26 ˚C) Temperature : ambient (26 ˚C) Detection : UV at 280 nm, 0.08 AUFS Detection : UV at 280 nm, 0.16 AUFS Injection : 15 µL (100 µL/1 vial) Injection : 15 µL (100 µL/1 vial) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD. Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD.

Proteins for molecular weight marker Proteins for molecular weight marker

1. Glutamate dehydrogenase (MW 290,000) 1. Glutamate dehydrogenase (MW 290,000) 2. Lactate dehydrogenase (MW 142,000) 2. Lactate dehydrogenase (MW 142,000) 3. Enolase (MW 67,000) 3. Enolase (MW 67,000) 4. Adenylate kinase (MW 32,000) 4. Adenylate kinase (MW 32,000) 5. Cytochrome c (MW 12,400) 5. Cytochrome c (MW 12,400)

(G911214B) (G911214C)

Column : YMC-Pack™ Diol-120 Column : YMC-Pack™ Diol-120 + Diol-300 500 x 8.0 mm I.D. 500 x 8.0 mm I.D. x 2 Part number : DL12S055008WT Part number : DL12S055008WT, DL30S055008WT Eluent : 0.1M KH PO - K HPO (pH 7.0) containing 0.2M NaCl 2 4 2 4 Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Flow rate : 0.7 mL/min Temperature : ambient (26 ˚C) Temperature : ambient (26 ˚C) Detection : UV at 280 nm, 0.08 AUFS Detection : UV at 280 nm, 0.08 AUFS Injection : 15 µL (100 µL/1 vial) Injection : 15 µL (100 µL/1 vial) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD. Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD.

Proteins for molecular weight marker Proteins

1. Ribonuclease A 2. Cytochrome c 1. Glutamate dehydrogenase (MW 290,000) 3. Lysozyme 2. Lactate dehydrogenase (MW 142,000) 5. Myoglobin 3. Enolase (MW 67,000) 4. Adenylate kinase (MW 32,000) 5. Cytochrome c (MW 12,400)

(T931029A)

(G911214H) Column : YMC- Pack™ ODS-A™ (5µm, 300Å) 150 x 4.6 mm I.D. Column : YMC-Pack™ Diol-200 + Diol-200 Part number : AA30S051546WT 500 x 8.0 mm I.D. + 300 x 8.0 mm I.D. Eluent : A) acetonitrile/water/TFA (5/95/0.1) Part number : DL20S055008WT, DL20S053008WT B) acetonitrile/water/TFA (60/40/0.1) 30-90%B (0-20min, linear), 90%B (20-25min) Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Flow rate : 1.0 mL/min Temperature : ambient (26 ˚C) Temperature : 37˚C Detection : UV at 280 nm, 0.08 AUFS Detection : UV at 220 nm, 0.32 AUFS Injection : 15 µL (100 µL/1 vial) Injection : 16 µL (0.16 ~ 0.33 mg/mL) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD.

1. [Arg8]- Vasopressin Proteins Peptides 2. Bradykinin 3. Angiotensin 1. Ribonuclease A 4. Oxytocin 2. Cytochrome c 5. Angiotensin 3. Lysozyme 6. Leu-Enkephalin 4. Myoglobin 5 6 3 COOH A) UV 4 1 2 OCH2CH3 p-Ethoxybenzoic acid

COOH

OCH2CH2CH3 p-Propoxybenzoic acid

3. COOH 0.0 5.00 10.00 15.00 20.00 min

CH CH 3 CH3 2 3 5 p-Isopropylbenzoic acid 6 (Cuminic acid) B) MS 4. COOH

1 4 OCH2CH2CH2CH3 p-Buthoxybenzoic acid

5. COOH

H C C CH 0.0 5.00 10.00 15.00 20.00 min 3 3 CH3 p-tert-Butylbenzoic acid (A020808A)

(B931023A) Column : YMC-Pack™ Pro C18 RS™ (5 µm, 80Å) 150 x 2.0 mm I. D. Column : YMC-Pack™ Butyl (5 µm, 300Å) Part number : RS08S051502WT 150 x 4.6 mm I. D. Eluent : A) water/TFA (100/0.01) Part number : BU30S051546WT B) acetonitrile/TFA (60/40/0.1) Eluent : A) acetonitrile/water/TFA (5/95/0.1) 10-35%B (0-15min), 10%B (15-20min) B) acetonitrile/water/TFA (60/40/0.1) Flow rate : 0.2 mL/min 30-90%B (0-20min, linear), 90%B (20-25min) Temperature : 37˚C Flow rate : 1.0 mL/min Detection : A) UV at 220 nm, 0.32 AUFS Temperature : 37˚C B) ESI positive-mode Detection : UV at 220 nm, 0.32 AUFS Injection : 4 µL ( 0.05 mg/mL) Injection : 16 µL (0.16 ~ 0.33 mg/mL)

Peptides

1. [Arg8]- Vasopressin 2. Oxytocin 1. COOH 3. Bradykinin 4. Angiotensin OCH2CH3 5. Leu-Enkephalin 5 p-Ethoxybenzoic acid 4 6 6. Angiotensin 2. COOH 2

1 OCH2CH2CH3 p-Propoxybenzoic acid

3. COOH 3 CH CH 3 CH3 p-Isopropylbenzoic acid (Cuminic acid)

4. COOH

OCH2CH2CH2CH3 p-Buthoxybenzoic acid

5. COOH

0 5 10 15 min H3C C CH3 CH3 p-tert-Butylbenzoic acid

(A010821A)

Column : YMC-Pack™ Pro C18 RS™ (5 µm, 80Å) 150 x 4.6 mm I. D. Part number : RS08S051546WT Eluent : A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 15-35%B (0-15min) Flow rate : 1.0 mL/min Temperature : 37˚C Detection : UV at 220 nm, 0.32 AUFS Injection : 20 µL (0.05 mg/mL)

Organic acids Organic acids

1. Glycolic acid 1. Tartaric acid 2. L-Malic acid 2. L-Malic acid 3. Malonic acid 3. Lactic acid 4. Lactic acid 4. Acetic acid 5. Acetic acid 5. Citric acid 6. Maleic acid 6. Succinic acid 7. Citric acid 8. Fumaric acid 9. Succinic acid 10. Propionic acid

(T910226A) (A001218A)

Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) Column : Hydrosphere™ C18 (5µm, 120Å) 250 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AQ12S051546WT Part number : HS12S051546WT Eluent : 20mM H PO - NaH PO (pH 2.8) 3 4 2 4 Eluent : 20mM NaH2PO4 - H3PO4(pH 2.8) Flow rate : 0.7 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 220 nm, 0.08 AUFS Detection : UV at 220 nm, 0.064 AUFS Injection : 10 µL (0.007~1.8 mg/mL) Injection : 10 µL (0.25 - 2.0 mg/mL)

Organic acids Pyruvic acids

1. L-Malic acid 2. Lactic acid 3. Acetic acid 4. Maleic acid 5. Succinic acid 6. Propionic acid 1. Phosphoenolpyruvic acid 7. Fumaric acid monopotassium salt

2. Pyruvic acid

(Y910209) (S921217A)

Column : YMC-Pack™ PolymerC18™ Column : YMC-Pack™ PolymerC18™ 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : PC99S062546WT Part number : PC99S062546WT

Eluent : 0.1% TFA Eluent : 100mM H3PO4 (pH 1.7) Flow rate : 0.5 mL/min Flow rate : 0.5 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 220 nm, 0.08 AUFS Detection : UV at 220 nm, 0.64 AUFS Injection : 10 µL (0.016~2.2 mg/mL) Injection : 5 µL (0.7, 20 mg/mL)

Unsaturated fatty acid methyl esters Methyl linolenate and methyl λ-linolenate

1. Eicosapentaenoic acid

2. É¡-Linolenic acid 1. Methyl linolenate 3. Docosahexaenoic acid

2. Methyl λ-linolenate 4. Arachidonic acid

5. Linoleic acid

6. Eicosatrienoic acid

(K930323A) (S921217A) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) ™ 250 x 4.6 mm I.D. Column : J’Sphere ODS-H80 (4µm, 80Å) Part number : AM12S052546WT 250 x 4.6 mm I.D. Eluent : acetonitrile/water (81/19) Part number : JH08S042546WT Flow rate : 1.5 mL/min Eluent : acetonitrile/THF/water (35/30/35) Temperature : ambient Flow rate : 1.0 mL/min Detection : UV at 190 nm, 0.128 AUFS Temperature : 37 ˚C Injection : 20 µL (0.165 mg/mL) Detection : UV at 210 nm, 0.26 AUFS Injection : 2 µL (2.5 µL/mL)

Unsaturated fatty acid methyl esters Triacylglycerols

1. LLL 2. LLO 3. POL 4. PPL 5. OOO 1 .Methyl linolenate 6. OOP 7. PPO 2. MethylÉ¡-linolenate L: linoleic acid O: oleic acid 3. Methyl linoleate P: palmitic acid

4. Methyl oleate

(S931012C) (O940914F) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. Column : J’Sphere™ ODS-H80 (4µm, 80Å) Part number : JH08S041546WT 250 x 4.6 mm I.D. Eluent : A)acetonitrile/THF/water (10/30/60) Part number : JH08S042546WT B)acetonitrile/THF/water(65/30/5) Eluent : acetonitrile/2-propanol (55/45) 20-100%B (0-40min, linear) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 210 nm, 0.08 AUFS Detection : UV at 210 nm, 0.51 AUFS Injection : 30 µL (0.08~1.66 mg/mL) Injection : 18 µL (0.25~4 µL/mL)

Food preservatives Food preservatives

1. Saccharin sodium 1. Dehydroacetic acid

2. Dehydroacetic acid 2. Sorbic acid

3. Sorbic acid

3. Benzoic acid

4. Benzoic acid

(O940819A) (O940802I)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 75 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : JH08S04L546WT Part number : JM08S04L546WT Eluent : THF/water/TFA (20/80/0.1) Eluent : THF/water/acetic acid (20/80/0.5) Flow rate : 1.0 mL/min Flow rate : 2.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 230 nm, 0.16 AUFS Detection : UV at 230 nm, 0.08 AUFS Injection : 2 µL (0.13~0.32 mg/mL)Text Injection : 4 µL (56 µg/mL)

Food Preservatives p-Hydroxybenzoic acid esters

1. Methyl p-hydroxybenzoate

2. Ethyl p-hydroxybenzoate 1. Dehydroacetic acid

3. Isopropyl p-hydroxybenzoate 2. Sorbic acid

4. Propyl p-hydroxybenzoate

3. Benzoic acid

5. Isobutyl p-hydroxybenzoate

6. Butyl p-hydroxybenzoate

(0940802H) (S930716D)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 75 x 2.0 mm I.D. 75 x 4.6 mm I.D. Part number : JM08S04L502WT Part number : JH08S04L546WT Eluent : THF/water/acetic acid (20/80/0.5) Eluent : methanol/water (55/45) Flow rate : 0.37 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 230 nm, 0.08 AUFS Detection : UV at 270 nm, 0.26 AUFS Injection : 0.8 µL (56 µg/mL Injection : 4 µL (0.1 mg/mL)

p-Hydroxybenzoic acid esters EDTA-chelates

1. Methyl p-hydroxybenzoate

2. Ethyl p-hydroxybenzoate 1. Fe (III)-EDTA

3. Propyl p-hydroxybenzoate

4. Butyl p-hydroxybenzoate 2. Cu (II)-EDTA

EDTE: Ethylenediamine- tetraacetic acid

(T940208A) (S930716H) Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : JH08S041546WT Part number : JM08S04L546WT Eluent : 50mM CH3COONa - CH3COOH (pH 4.6) containing Eluent : acetonitrile/water (50/50) 20mM tetrabutylammonium bromide Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 270 nm, 0.26 AUFS Detection : UV at 254 nm, 0.04 AUFS Injection : 3 µL (0.1 mg/mL) Injection : 10 µL (0.017~0.13 mg/mL)

Antioxidants Antioxidants

5. 4-Hydroxymethyl 1. n-Propyl gallate(PG) 5. 4-Hydroxymethyl- 1. n-Propyl gallate(PG) 2,6-di-tert-butylphenol 2,6-di-tert-butylphe- (HMBP) nol (HMBP)

2. 2,4,5-Trihydroxy 6. n-Octyl gallate(OG) 2. 2,4,5-Trihydroxy 6. n-Octyl butyrophenone butyrophenone gallate(OG) (THBP) (THBP)

7. n-Dodecyl 3. tert-Butylhydroquinone 7. n-Dodecyl gal- 3. tert-Butylhydroquinone gallate(DG) (TBHQ) (TBHQ) late(DG)

4. Nordihydroguaiaretic 4. Nordihydroguaiaretic acid(NDGA) acid(NDGA)

(A970524B) (A970526C)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 150 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : AS12S051546WT Part number : AS12S05L546WT Eluent : A)acetonitrile/methanol/5% acetic acid (20/20/60) Eluent : A)acetonitrile/methanol/5% acetic acid (20/20/60) B)acetonitrile/methanol/5% acetic acid (45/45/10) B)acetonitrile/methanol/5% acetic acid (45/45/10) 0-100%B (0-10min), 100%B (10-20min) 0-100%B (0-5min), 100%B (5-15min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 280 nm, 0.32 AUFS Detection : UV at 280 nm, 0.32 AUFS Injection : 9 µL (0.05~0.6 mg/mL) Injection : 9 µL (0.05~0.6 mg/mL)Text

Antioxidants Antioxidants

1. n-Propyl gallate(PG) 1. n-Propyl gallate(PG)

2. 2,4,5-Trihydroxybutyrophenone (THBP) 2. 2,4,5-Trihydroxybutyrophenone (THBP)

3. tert-Butylhydroquinone(TBHQ) 3. tert-Butylhydroquinone(TBHQ)

(A970522B) (A970522D)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : AQ12S051546WT Eluent : acetonitrile/methanol/5% acetic acid (20/20/60) Eluent : acetonitrile/methanol/5% acetic acid (20/20/60) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 280 nm, 0.32 AUFS Detection : UV at 280 nm, 0.32 AUFS Injection : 10 µL (0.05~0.2 mg/mL) Injection : 10 µL (0.05 ~ 0.2 mg/mL

Antioxidants Antioxidants

1. Nordihydroguaiaretic acid(NDGA)

2. 4-Hydroxymethyl-2,6-di-tert-butylphenol (HMBP)

3. n-Dodecyl gallate(DG)

(A970521B) (J010215B)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : Hydrosphere™ C18 (3µm, 120Å) 150 x 4.6 mm I.D. 50 x 4.6 mm I.D. Part number : AS12S051546WT Part number : HS12S030546WT Eluent : acetonitrile/methanol/5% acetic acid (35/35/30) Eluent : acetonitrile / methanol / 5% acetic acid (35/35/30) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 280 nm, 0.32 AUFS Detection : UV at 280 nm, 0.32 AUFS Injection : 10 µL (0.1~0.6 mg/mL) Injection : 10 µL (0.05 - 0.5 mg/mL)

Antioxidants Color additives 1. Tartrazine (FD&C Yellow No.5, C.I.19140, Y-4) 2. Amaranth (C.I.16185, R-2) 3. Indigo Carmine (FD&C Blue No.2, C.I.73015, B-2) 4. New Coccine (C.I.16255, R-102) 1. Butyl hydroxyanisol (BHA) 5. Sunset Yellow FCF (FD&C Yellow No.6, C.I.15985, Y-5) 6. Fast Green FCF (FD&C Green No.3, C.I.42053, G-3) 7. Brilliant Blue FCF (FD&C Blue No.1, C.I.42090, B-1) 8. Erythrosine (FD&C Red No.3, C.I.45430, R-3) 9. Acid Red 2. Dibutyl hydroxytoluene (BHT) (C.I.45100, R-106) 10. Phloxine (C.I.45410, R-104) 11. Rose Bengale (C.I.45440, R-105)

(M910427A)

(O940810D Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 4.6 mm I.D. Column : J’Sphere™ ODS-H80 (4µm, 80Å) Part number : AA12S051546WT

75 x 4.6 mm I.D. Eluent : A) 10mM NH4H2PO4 - (NH4)2HPO4 (pH 6.0)/methanol (90/10)

Part number : JH08S04L546WT B) 10mM NH4H2PO4 - (NH4)2HPO4 (pH 6.0)/methanol (20/80) Eluent : methanol/water (95/5) 0-100%B (0-40min, linear), 100%B (40-60min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 280 nm, 0.16 AUFS Detection : UV at 254 nm, 0.32 AUFS Injection : 1 µL (0.5 mg/mL) Injection : 100 µL (5 µg/mL)

Color additives Color additives

1. Fast Green FCF 1. Tartrazine (FD&C Green NO.3, C.I.42053, G-3) (FD&C Yellow No.5, C.I.19140,Y-4) 2. Brilliant Blue FCF 2. Amaranth (FD&C Blue No.1, C.I.42090, B-1) (C.I.16185, R-2) 3. Erythrosine 3. Indigo Carmine (FD&C Red No.3, C.I.45430, R-3) (FD&C Blue No.2, C.I.73015, B-2) 4. Acid Red 4. New Coccine (C.I.45100, R-106) (C.I.16255, R-102) 5. Phloxine 5. Sunset Yellow FCF (C.I.45410, R-104) (FD&C Yellow No.6, C.I.15985, Y-5) 6. Rose Bengale (C.I.45440, R-105)

(M910430C) (M910430A)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column :YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : AA12S051546WT Eluent : acetonitrile/10mM CH COONH (25/75) 3 4 Eluent : acetonitrile/10mM CH COONH (8/92) Flow rate : 1.0 mL/min 3 4 Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.08 AUFS Detection : UV at 254 nm, 0.16 AUFS Injection : 10 µL (35~140 µg/mL) Injection : 5 µL (25~200 µg/mL)

Annatto extract Aspartame in a soft drink

1. Annatto pigment 1. Benzoic acid 2. Caffeine 3. Aspartame

(M910430E) (T910123) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 150 x 4.6 mm I.D. Column : YMC-Pack™ Phenyl (5µm, 120Å) Part number : AM12S051546WT 150 x 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (65/34/1) Part number : PHI2S051546WT Flow rate : 1.0 mL/min Eluent : 50mM KH2PO4/acetonitrile/methanol (84/3/13) Temperature : 30 ˚C Flow rate : 1.5 mL/min Detection : VIS at 454 nm, 0.16 AUFS Temperature : 30 ˚C Injection : 5 µL (2 mg/mL) Detection : UV at 214 nm, 0.64 AUFS Sample : Annatto pigment Injection : 10 µL

Aspartame in a soft drink Aspartame in a soft drink

1. Caffeine 1. Caffeine 2. Aspartame 2. Aspartame 3. Benzoic acid 3. Benzoic acid

(H900710A) (H900710B)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ PolymerC18™ 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : PC99S061546WT Eluent : acetonitrile/water/TFA (15/85/0.05) Eluent : acetonitrile/water/TFA (15/85/0.05) Flow rate : 0.5 mL/min Flow rate : 0.5 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 220 nm, 0.64 AUFS Detection : UV at 220 nm, 0.64 AUFS Injection : 5 µL Injection : 5 µL

Aspartame in a soft drink Stevioside and rebaudioside A

1. Caffeine 1. Stevioside 2. Aspartame 2. Rebaudioside A 3. Benzoic acid

(H900710C) (G900727B)

Column : YMC™-Pack CN (5µm, 120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 250 x 4.6 mm I.D. 150 x 6.0 mm I.D. Part number : CN12S052546WT Part number : AQ12S051506WT Eluent : acetonitrile/water/TFA (15/85/0.05) Eluent : acetonitrile/water (35/65) Flow rate : 0.5 mL/min Flow rate : 0.5 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 220 nm, 0.64 AUFS Detection : UV at 205 nm, 0.32 AUFS Injection : 5 µL Injection : 7 µL

Glycyrrhizic acid Food preservatives

1. COOH

Benzoic acid 1

CH3CH CHCH CHCOOH 1. Glycyrrhizic acid 2 Sorbic acid 3

3. H3C O O

COCH3 O Dehydroacetic acid 2. Propyl p-hydroxybenzoate 0 5 10 15 min

(S960306E) (J011213F)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ Pro C18 RS™ (5µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : RS08S051546WT

Eluent : acetonitrile/water/acetic acid (40/56/4) Eluent : 20mM CH3COONa - CH3COOH (pH 4.3)/ acetonitrile (80/20) Flow rate : 0.6 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 1.0 AUFS Detection : UV at 230 nm, 0.26 AUFS Injection : 10 µL (0.1~0.5 mg/mL) Injection : 10 µL (0,02 mg/mL)

Alkaloids Alkaloids

1. Hordenine sulfate 1. Hordenine sulfate 2. Pilocarpine chloride 2. Pilocarpine chloride 3. Scopolamine bromide 3. Scopolamine bromide 4. Atropine sulfate 4. Atropine sulfate 5. Cinchonine 5. Cinchonine 6. Sparteine sulfate 6. Sparteine sulfate 7. Quinine 7. Quinine 8. Papaverine chloride 8. Papaverine chloride 9. Narcotine chloride 9. Narcotine chloride

(G910523K) (G910523J) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 150 x 6.0 mm I.D. Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Part number : AM12S051506WT 150 x 4.6 mm I.D. Eluent : A)acetonitrile/20mM KH PO (17/83) Part number : AM12S051546WT 2 4 B)acetonitrile/20mM KH PO (40/60) Eluent : acetonitrile/20mM KH PO (20/80) 2 4 2 4 0-100%B (0-30min, linear), 100%B (30-40min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30˚C Temperature : 30 ˚C Detection : UV at 210 nm, 0.16 AUFS Detection : UV at 210 nm, 0.16 AUFS Injection : 5 µL (0.05~2.19 mg/mL) Injection : 5 µL (0.03 ~ 2.19 mg/mL)

Purine alkaloids Chlorophyll a and b

1. Trigonerlline 2. Theobromine 3. Theophylline 4. Caffeine 5. Phenol

1. Chlorophyll b

2. Chlorophyll a

Courtesy of Dr. Toyoaki Aoki, University of Osaka Pref. (K940228A) (M910612C) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 150 x 6.0 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051506WT Part number : AQ12S051546WT Eluent : methanol Eluent : acetonitrile/water (8/92) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient Temperature : 20 ˚C Detection : VIS at 450 nm, 1 AUFS Detection : UV at 254 nm, 0.32 AUFS Injection : 100 µL Injection : 5 µL (0.3 ~ 4.5 mg/mL) Sample : extract of Spinacia with acetone/water (90/10)

Crude drugs(Glycosides) Crude drugs(Glycosides)

1. Arbutin 1. Arbutin 2. Salicin 2. Salicin 3. Esculine 3. Esculine 4. G-Strophanthin (Ouabain) 4. G-Strophanthin (Ouabain) 5. Baicalin 5. Baicalin 6. Barbaloin 6. Barbaloin 7. Glycyrrhizin 7. Glycyrrhizin 8. Digitoxin 8. Digitoxin

(G910607D) (G910607E)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : AA12S052546WT Part number : AQ12S052546WT

Eluent : A)methanol/20mM NH4H2PO4 (5/95) Eluent : A)methanol/20mM NH4H2PO4 (5/95)

B)methanol/20mM NH4H2PO4 (80/20) B)methanol/20mM NH4H2PO4 (80/20) 0-100%B (0-20min, linear), 100%B (20-40min) 0-100%B (0-20min, linear), 100%B (20-40min) Flow rate : 0.6 mL/min Flow rate : 0.6 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 250 nm, 0.08 AUFS Detection : UV at 250 nm, 0.08 AUFS Injection : 18 µL (0.01~0.49 mg/mL) Injection : 18 µL (0.01 ~ 0.49 mg/mL)

Paeoniflorin Paeoniflorin

1. Albiflorin 1. Paeoniflorin 2. Paeoniflorin 3. p-Hydroxyacetophenone(I.S.)

2. p-Hydroxyacetophenone(I.S.)

(A980818A) (O941121M)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : AS12S051546WT Part number : JH08S04L546WT Eluent : acetonitrile/water/acetic acid (20/80/1) Eluent : THF/50mM (NH4)2HPO4 (10/90) Flow rate : 0.4 mL/min Flow rate : 1.0 mL/min Temperature : 25 ˚C Temperature : 37 ˚C Detection : UV at 230 nm, 0.16 AUFS Detection : UV at 230 nm, 0.16 AUFS Injection : 10 µL (48 - 56 µg/mL) Injection : 3 µL (0.67, 0.33 mg/mL)

Paeoniflorin in Toki-shakuyaku-san Saponins from roots of rubus suavissimus S. Lee

1. 1. Paeoniflorin

Courtesy of Dr.Ohtani,Hiroshima University,Japan (Y910123)

(O941121I) Column : YMC-Pack™ PolymerC18™ and YMC-Pack™ ODS-AM™ (5µm, 120Å) 150 x4.6 mm I.D. Column : J’Sphere™ ODS-H80 (4µm, 80Å) Part number : PC99S061546WT, AM12S051546WT 150 x 4.6 mm I.D. Eluent : methanol/water (45/55) for PolymerC18 ™ Part number : JH08S041546WT methanol/water (50/50) for ODS-AM Eluent : THF/50mM (NH ) HPO (10/90) Flow rate : 0.8 mL/min 4 2 4 Temperature : 50 ˚C Flow rate : 1.0 mL/min Detection : UV at 210 nm, 0.32 AUFS Temperature : 37 ˚C Sample : 1) β-D-Glucopyranosyl 2α, 3, β,23-trihydroxyurs- Detection : UV at 230 nm, 0.16 AUFS 12,18-dien-28-oate Injection : 3 µL 2) β -D-Glucopyranosyl 2α, 3, β,23-trihydroxyurs- Sample : water extract from Toki-shakuyaku-san 12,19-dine-28-oate

Saikosaponins Saikosaponins

1. Saikosaponin c 1. Saikosaponin c

2. Saikosaponin a 2. Saikosaponin a

3. Saikosaponin b2 3. Saikosaponin b2

4. Saikosaponin d 4. Saikosaponin d (S931210D) (S931210C)

Column : J’Sphere™ ODS-L80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JL08S041546WT Part number : JM08S041546WT Eluent : acetonitrile/water (38/62) Eluent : acetonitrile/water (38/62) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 210 nm, 0.26 AUFS Detection : UV at 210 nm, 0.26 AUFS Injection : 8 µL (0.5~0.75 mg/mL) Injection : 8 µL (0.5 ~ 0.75 mg/mL)

Ginsenosides Ginsenosides

1. Ginsenoside-Rg1

2. Ginsenoside-Rb1

(S921217A) (A971120C)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 150 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : AQ12S051546WT Eluent : acetonitrile/water (30/70) Eluent : acetonitrile/water (30/70) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 203 nm, 0.16 AUFS Detection : UV at 203 nm, 0.16 AUFS Injection : 10 µL (0.125, 0.5 mg/mL) Injection : 10 µL (0.125, 0.5 mg/mL)

Ginsenosides Flavonoids

1. Myricetin

2. Quercetin

1. Ginsenoside-Rg1

3. Kaempferol

2. Ginsenoside-Rb1

(A971121D)

™ ™ Column : YMC-Pack Pro C18 (5µm, 120Å) (D960722A) 150 x3.0 mm I.D. Part number : AS12S051503WT Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Eluent : A)acetonitrile/water (20/80) 150 x 4.6 mm I.D. B)acetonitrile/water (40/60) Part number : AS12S051546WT 0-100%B (0-10min), 100%B (10-20min) Eluent : acetonitrile/10mM H3PO4 (30/70) Flow rate : 0.4 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 40 ˚C Detection : UV at 203 nm, 0.32 AUFS Detection : UV at 260 nm, 0.064 AUFS Injection : 4 µL (0.125, 0.5 mg/mL) Injection : 2 µL (0.05 mg/mL)

Flavonoids Flavonoids

1. Luteolin 1. Luteolin

2. Apigenin 2. Apigenin

3. Chrysoeriol 3. Chrysoeriol

4. Baicalein 4. Baicalein

5. Chrysin 5. Chrysin

(S961107C) 6. Acacetin 6. Acacetin (S961111B)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : AQ12S051546WT Eluent : acetonitrile/10mM H PO (35/65) 3 4 Eluent : acetonitrile/10mM H3PO4 (35/65) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 280 nm, 0.13 AUFS Detection : UV at 280 nm, 0.13 AUFS Injection : 5 µL (0.05 mg/mL) Injection : 5 µL (0.05 mg/mL)

Baicalin Flavanone glycosides

1. Narirutin

1. Baicalin

2. Naringin

2. Methyl p-hydroxybenzoate

3. Hesperidin

4. Neohesperidin

(S961105C) (A970430A)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 50 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S050546WT Part number : AS12S051546WT

Eluent : acetonitrile/10mM H3PO4 (30/70) Eluent : acetonitrile/10mM H3PO4 (20/80) Flow rate : 0.7 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 280 nm, 0.064 AUFS Detection : UV at 280 nm, 0.04 AUFS Injection : 10 µL (0.01, 0.02 mg/mL) Injection : 7 µL (0.025 mg/mL)

Flavanone glycosides Isoflavones from miso,fermented soybean paste

1. Narirutin

2. Naringin

3. Hesperidin

(F001025B) 4. Neohesperidin

Column : Hydrosphere™ C18 (5µm, 120Å) (A970506A) 150 x 4.6 mm I.D. Part number : HS12S051546WT Column : J’Sphere™ ODS-H80 (4µm, 80Å) Eluent : A)water/acetic acid (100/1) 150 x 4.6 mm I.D. B)acetonitrile/water/acetic acid (50/50/1) Part number :JH08S041546WT 30%B(0-5min), 30-70%B(5-35min) Flow rate : 1.0 mL/min Eluent : acetonitrile/10mM H3PO4 (20/80) Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.08AUFS Detection : UV at 280 nm, 0.04 AUFS Injection : 7 µL Injection : 7 µL (0.025 mg/mL) Sample : methanol extract of miso

Catechins Catechins

1. (-)-Epigallocatechin

2. (+)-Catechin

3. (-)-Epigallocatehin gallate

4. (-)-Epicatechin

5. (-)-Epicatechin gallate (F000828A) (S940124A)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : JH08S041546WT Eluent : acetonitrile/ethyl acetate/water/TFA (9/3/88/0.1) Eluent : 20mM KH2PO4 - H3PO4 (pH 2.4)/methanol (75/25) Flow rate : 1.0 mL/min Flow rate : 0.8 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 280 nm, 0.32 AUFS Detection : UV at 280 nm, 0.13 AUFS Injection : 7 µL (0.2 - 0.8 mg/mL) Injection : 8 µL (0.1~0.4 mg/mL)

Catechins 2-Phenylethyl glycosides

1. (-)-Epigallocatechin

2. (+)-Catechin

3. (-)-Epigallocatehin gallate

4. (-)-Epicatechin

5. (-)-Epicatechin gallate (S940122D) (F000911A)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : Hydrosphere™ C18 (5µm, 120Å) 150 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : JM08S041546WT Part number :HS12S052546WT

Eluent : 20mM KH2PO4 - H3PO4 (pH 2.4)/methanol (75/25) Eluent : acetonitrile/methanol/water (8/4/88) Flow rate : 0.8 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 280 nm, 0.13 AUFS Detection : UV at 210 nm, 0.064 AUFS Injection : 8 µL (0.1~0.4 mg/mL) Injection : 5 µL (0.2 mg/mL)

Sennosides Sennosides

1. Sennoside B 1. Sennoside B

2. Sennoside A 2. Sennoside A

(A961126C) (A961126B)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : JH08S041546WT Eluent : methanol/water/acetic acid (30/65/5) Eluent : methanol/water/acetic acid (30/65/5) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 340 nm, 0.16 AUFS Detection : UV at 340 nm, 0.16 AUFS Injection : 10 µL (0.1 mg/mL) Injection : 10 µL (0.1 mg/mL)

Sennosides Arbutin and related compounds

1. Arbutin

1. Sennoside B

2. Hydroquinone

2. Sennoside A

3. Gallic acid (A961126D) (A970514D) Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JM08S041546WT Part number : AS12S051546WT Eluent : methanol/water/acetic acid (30/65/5) Eluent : methanol/10mM H PO (5/95) Flow rate : 1.0 mL/min 3 4 Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 340 nm, 0.16 AUFS Detection : UV at 280 nm, 0.32 AUFS Injection : 10 µL (0.1 mg/mL Injection : 5 µL (0.2~0.5 mg/mL)

Arbutin and related compounds Arbutin and related compounds

1. Arbutin

2. Hydroquinone

3. Gallic acid

(A970514B) (A980831L)

Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) Column : YMC-Pack™ Pro C18™ (3µm, 120Å) 150 x 4.6 mm I.D. 75 x 2.0 mm I.D. Part number : AQ12S051546WT Part number : AS12S03L502WT

Eluent : methanol/10mM H3PO4 (5/95) Eluent : methanol / water / TFA (5/95/0.05) Flow rate : 1.0 mL/min Flow rate : 0.2 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 280 nm, 0.32 AUFS Detection : UV at 280 nm, 0.16 AUFS Injection : 5 µL (0.2 ~ 0.5 mg/mL) Injection : 1 µL (0.2 - 0.5 mg/mL)

Paeonol Paeonol

1. Paeonol 1. Paeonol

2. Butyl p-hydroxybenzoate 2. Butyl p-hydroxybenzoate

(S971106D) (S971107A)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : AQ12S051546WT Eluent : acetonitrile/water/acetic acid (35/65/2) Eluent : acetonitrile/water/acetic acid (35/65/2) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (22 ˚C) Temperature : ambient (22 ˚C) Detection : UV at 274 nm, 0.26 AUFS Detection : UV at 274 nm, 0.26 AUFS Injection : 10 µL (0.04, 0.2 mg/mL) Injection : 10 µL (0.04, 0.2 mg/mL)

Aflatoxins Aflatoxins

1. Aflatoxin G2 1. Aflatoxin G2

2. Aflatoxin G1 2. Aflatoxin G1

3. Aflatoxin B2 3. Aflatoxin B2

4. Aflatoxin B1 4. Aflatoxin B1

(K931124B) (K931125A)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JM08S041546WT Part number : JL08S041546WT Eluent : methanol/water (40/60) Eluent : methanol/water (40/60) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 365 nm, 0.04 AUFS Detection : UV at 365 nm, 0.04 AUFS Injection : 10 µL (7~10 ppm) Injection : 10 µL (7 ~ 10 ppm)

Steroids Steroids

1. Progesterone 1. Hydrocortisone

2. Deoxycorticosterone 2. Corticosterone

3. Deoxycorticosterone 3. Corticosterone

4. Progesterone 4. Hydrocortisone

(S930322B) (S930319D) Column : YMC-Pack™ CN (5µm, 120Å) Column : YMC-Pack™ CN (5µm, 120Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : CN12S052546WT Part number : CN12S052546WT Eluent : acetonitrile/water (40/60) Eluent : hexane/methanol/dichloromethane (75/20/5) Flow rate : 0.6 mL/min Flow rate : 0.6 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.32 AUFS Detection : UV at 254 nm, 0.32 AUFS Injection : 3 µL (0.25 mg/mL) Injection : 3 µL (0.25 mg/mL)

Adrenocorticosteroids Adrenocorticosteroids

1. Prednisolone 6. Hydrocortisone acetate

1. Prednisone 4. Hydrocortisone

2. Hydrocortisone 7. Cortisone acetate

2. Cortisone 5. Corticosterone 3. Cortisone 8. Deoxycorticosterone

3. Prednisolone 6. Methylprednisolone 4. Corticosterone 9. Corticosterone acetate

10. Benzene

5. Fluocinolone acetonide

(S940308B) (S940222C)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : JM08S041546WT Eluent : THF/water (20/80) Eluent : acetonitrile/water (35/65) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.13 AUFS Detection : UV at 260 nm, 0.13 AUFS Injection : 8 µL (0.1 mg/mL) Injection : 7 µL (0.07 mg/mL)

Adrenocorticosteroids Adrenocorticosteroids

1. Hydrocortisone 5. Dexamethasone 1. Hydrocortisone 5. Dexamethasone

2. Cortisone 6. Corticosterone 2. Cortisone 6. Corticosterone

7. Beclomethasone 7. Beclomethasone 3. Methylprednisolone 3. Methylprednisolone

8. Triamcinolone acetonide 4. Betamethasone 4. Betamethasone 8. Triamcinolone acetonide

(S940310E) (S940310F)

Column : J’Sphere™ ODS-H80 (4 µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : JM08S041546WT Eluent : acetonitrile/water (27/73) Eluent : acetonitrile/water (27/73) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.13 AUFS Detection : UV at 260 nm, 0.13 AUFS Injection : 10 µL (0.08 mg/mL Injection : 10 µL (0.08 mg/mL)

Hydrocortisone and prednisone Adrenocorticosteroids

1. Fluocinolone acetonide

1. Prednisone

2. Prednisolone acetate

2. Hydrocortisone

3. Deoxycorticosterone

4. Fluocinonide (K940530C) (O940408A)

Column : YMC-Pack™ SIL (5µm, 120Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : SL12S051546WT Part number : JM08S041546WT Eluent : chloroform/2-propanol/acetic acid (95/5/1) Eluent : acetonitrile/water (45/55) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 250 nm, 0.32 AUFS Detection : UV at 260 nm, 0.13 AUFS Injection : 10 µL (0.25 mg/mL) Injection : 5 µL (0.1 mg/mL)

Adrenocorticosteroids Adrenocorticosteroids

1. Fluocinolone acetonide 1. Triamcinolone

2. Prednisolone 2. Prednisolone acetate

3. Triamcinolone acetonide 3. Deoxycorticosterone

4. Fluocinonide

(O940408C) (S940420B)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : JH08S041546WT Eluent : acetonitrile/water (45/55) Eluent : acetonitrile/water (30/70) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.13 AUFS Detection : UV at 260 nm, 0.13 AUFS Injection : 5 µL (0.1 mg/mL) Injection : 4 µL (0.1~ 0.2 mg/mL)

Adrenocorticosteroids Adrenocorticosteroids

1. Triamcinolone 1. Prednisolone succinate

2. Prednisolone 2. Hydrocortisone succinate

3. Triamcinolone acetonide

(S940420C) (S940414A)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JM08S041546WT Part number : JH08S041546WT Eluent : acetonitrile/water (30/70) Eluent : acetonitrile/water/acetic acid (30/70/1) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.13 AUFS Detection : UV at 260 nm, 0.064 AUFS Injection : 4 µL (0.1 ~ 0.2 mg/mL) Injection : 5 µL (0.1 mg/mL)

Adrenocorticosteroids Adrenocorticosteroids

1. Prednisone 1. Prednisone 2. Cortisone 2. Cortisone 3. Prednisolone 3. Prednisolone 4. Hydrocortisone 4. Hydrocortisone 5. Methylprednisolone 5. Methylprednisolone 6. Corticosterone 6. Corticosterone 7. Betamethasone 7. Betamethasone 8. Dexamethasone 8. Dexamethasone 9. Triamcinolone acetonide 9. Triamcinolone acetonide 10. Beclomethasone 10. Beclomethasone 11. Fluocinolone acetonide 11. Fluocinolone acetonide 12. Prednisolone acetate 12. Prednisolone acetate 13. Hydrocortisone acetate 13. Hydrocortisone acetate 14. Cortisone acetate 14. Cortisone acetate 15. Deoxycorticosterone 15. Deoxycorticosterone 16. Corticosterone acetate 16. Corticosterone acetate 17. Fluocinonide 17. Fluocinonide 18. Betamethasone valerate 18. Betamethasone valerate 19. Deoxycorticosterone acetate 19. Deoxycorticosterone acetate 20. Beclomethasone dipropionate 20. Beclomethasone dipropionate 21. Ethylbenzene 21. Ethylbenzene

(S940303D) (S940303B)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : JM08S041546WT Eluent : A)acetonitrile/THF/water (15/10/75) Eluent : A)acetonitrile/THF/water (15/10/75) B)acetonitrile/water (75/25) B)acetonitrile/water (75/25) 0-50%B (0-30min, linear), 50-100%B (30-50min) 0-50%B (0-30min, linear), 50-100%B (30-50min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.13 AUFS Detection : UV at 260 nm, 0.13 AUFS Injection : 10 µL (0.045 mg/mL) Injection : 10 µL (0.045 mg/mL)

Adrenocorticosteroids Steroids 1. Prednisone 2. Cortisone 3. Prednisolone 4. Hydrocortisone 5. Methylprednisolone 6. Corticosterone 7. Betamethasone 8. Dexamethasone 1. Fluoxymesterone 9. Triamcinolone acetonide 10. Beclomethasone 11. Fluocinolone acetonide 12. Prednisolone acetate 13. Hydrocortisone acetate 14. Cortisone acetate 15. Deoxycorticosterone 16. Corticosterone acetate 2. Methylprednisolone 17. Fluocinonide 18. Betamethasone valerate 19. Deoxycorticosterone acetate 20. Beclomethasone dipropionate 21. Ethylbenzene

3. Prednisolone

(S940303C)

Column : J’Sphere™ ODS-L80 (4µm, 80Å) (K940602B) 150 x 4.6 mm I.D. Column : YMC-Pack™ SIL-60A (5µm, 6Å) Part number : JL08S041546WT 250 x 4.6 mm I.D. Eluent : A)acetonitrile/THF/water (15/10/75) Part number : SL06S052546WT B)acetonitrile/water (75/25) Eluent : hexane/THF/acetic acid (60/40/1) 0-50%B (0-30min, linear), 50-100%B (30-50min) Flow rate : 1.5 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 250 nm, 0.16 AUFS Detection : UV at 260 nm, 0.13 AUFS Injection : 10 µL (0.25 mg/mL) Injection : 10 µL (0.045 mg/mL)

Androgens Androgens

1. Fluoxymesterone 4. Methyltestosterone 1. Fluoxymesterone 4. Methyltestosterone

2. Testosterone 5. trans -Androsterone 2. Testosterone 5. trans -Androsterone

3. Dehydroisoandrosterone 6. Androsterone 3. Dehydroisoandrosterone 6. Androsterone

(S930707D) (S930707C)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : JM08S041546WT Eluent : methanol/acetonitrile/water (45/15/40) Eluent : methanol/acetonitrile/water (45/15/40) Flow rate : 0.8 mL/min Flow rate : 0.8 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 210 nm, 0.064 AUFS Detection : UV at 210 nm, 0.064 AUFS Injection : 10 µL (0.1 ~ 2.0 mg/mL) Injection : 10 µL (0.1 ~ 2.0 mg/mL)

Androgens Synthetic androgens

1. Fluoxymesterone 4. Methyltestosterone 1. Testosterone

2. Testosterone 5. trans -Androsterone 2. Testosterone propionate

3. Dehydroisoandrosterone 6. Androsterone

3. Testosterone enanthate

(S930706G) (S940412B)

Column : J’Sphere™ ODS-L80 (4µm, 80Å) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 150 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : JL08S041546WT Part number : JL08S04L546WT Eluent : methanol/acetonitrile/water (45/15/40) Eluent : acetonitrile/water (70/30) Flow rate : 0.8 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 210 nm, 0.064 AUFS Detection : UV at 260 nm, 0.13 AUFS Injection : 10 µL (0.1 ~ 2.0 mg/mL) Injection : 5 µL (0.1 ~ 0.25 mg/mL)

Synthetic androgens Estrogens and progestins

1. Estriol 5. Estrone

1. Testosterone

2. β-Estradiol 6. Progesterone

2. Testosterone propionate

3. Norethisterone 7. Medroxyprogesterone acetate

3. Testosterone enanthate

4. Ethinylestradiol 8. Mestranol

(S940412C) (S930311D)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 75 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : JM08S04L546WT Part number : AA12S052546WT Eluent : acetonitrile/water (70/30) Eluent : acetonitrile/THF/water (46/4/50) Flow rate : 1.0 mL/min Flow rate : 0.7 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 260 nm, 0.13 AUFS Detection : UV at 230 nm, 0.16 AUFS Injection : 5 µL (0.1 ~ 0.25 mg/mL) Injection : 7 µL (0.1 mg/mL)

Estrogens and progestins Estrogens

1. Estriol 5. Estrone

1. β-Estradiol

2. β-Estradiol 6. Progesterone

2. Estradiol benzoate

3. Norethisterone 7. Medroxyprogesterone acetate

3. Estradiol dipropionate

4. Ethinylestradiol 8. Mestranol

(S930312C) (S940428E)

Column : YMC-Pack™ C8 (5µm, 120Å) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 250 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : OC12S052546WT Part number : JL08S041546WT Eluent : acetonitrile/THF/water (46/4/50) Eluent : acetonitrile/water (65/35) Flow rate : 0.7 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 230 nm, 0.16 AUFS Detection : UV at 230 nm, 0.13 AUFS Injection : 7 µL (0.1 mg/mL) Injection : 6 µL (0.08 ~ 0.5 mg/mL)

Estrogens Norgestrel and ethinylestradiol

1. Ethinylestradiol 1. β-Estradiol

2. Norgestrel

2. Estradiol benzoate

3. Diphenyl(I.S.)

3. Estradiol dipropionate

(S940428F) (S940427B)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 75 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : JM08S04L546WT Part number : JL08S04L546WT Eluent : acetonitrile/water (65/35) Eluent : acetonitrile/water (50/50) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 230 nm, 0.13 AUFS Detection : UV at 230 nm, 0.13 AUFS Injection : 6 µL (0.08 ~ 0.5 mg/mL) Injection : 6 µL (0.05 ~ 0.1 mg/mL)

Norgestrel and ethinylestradiol Phenacyl esters of bile acids from pig gall

1. Cholic acid (CA) 1. Ethinylestradiol 2. Hyodeoxycholic acid (HDCA) 3. Chenodeoxycholic acid (CDAC)

2. Norgestrel

3. Diphenyl(I.S

(G900417B) (S940426A) Column : YMC-Pack™ ODS-AL™ (5µm, 120Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 250 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number :AL12S052546WT Part number : JM08S04L546WT Eluent : acetonitrile/water (60/40) Eluent : acetonitrile/water (50/50) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 40 ˚C Temperature : 37 ˚C Detection : UV at 240 nm, 0.04 AUFS Detection : UV at 230 nm, 0.13 AUFS Sample : extract of pig gall were derivatized with phenacyl bromide Injection : 6 µL (0.05 ~ 0.1 mg/mL Injection : 5 µL

Ecdysones Sterols

1. β --Ecdysone 2. α -Ecdysone

1. Brassicasterol 4. Stigmasterol

2. Cholesterol 5. Campesterol

3. Fucosterol 6. β-Sitosterol

(O940701C) Courtesy of Dr.Furusawa, Kyoto Institute of Technology, Japan (G910523H) Column : YMC-Pack™ ODS-AL™ (5µm, 120Å) x 3 + J’Sphere™ ODS-M80 (4µm, 80Å) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) (250 x 4.6 mm I.D.) x 4 150 x 4.6 mm I.D. Part number : AL12S052546WT, JM08S042546WT Part number : AM12S051546WT Eluent : methanol/water (100/2) Eluent : acetonitrile/methanol/water(5/50/45) Flow rate : 0.8 mL/min Flow rate : 0.5 mL/min Temperature : 37 ˚C Temperature : ambient Detection : UV at 210 nm, 0.16 AUFS Detection : UV at 254 nm, 0.04 AUFS Injection : 10 µL (0.4 ~ 1 mg/mL) Injection : 5 µL (0.04 mg/mL)

Sterols Sterols

1. Brassicasterol 1. Brassicasterol

2. Cholesterol 2. Cholesterol

3. Fucosterol 3. Fucosterol

4. Stigmasterol 4. Stigmasterol

5. Campesterol 5. Campesterol

6. β-Sitosterol (O940706H) 6. β-Sitosterol (O940706C)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : YMC-Pack™ ODS-AL™ (5µm, 120Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : JM08S042546WT Part number : AL12S052546WT Eluent : methanol/water (100/2) Eluent : methanol/water (100/2) Flow rate : 0.8 mL/min Flow rate : 0.8 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 210 nm, 0.64 AUFS Detection : UV at 210 nm, 0.32 AUFS Injection : 10 µL (0.4 ~ 1 mg/mL) Injection : 10 µL (0.4 ~ 1 mg/mL)

Cardiac glycosides Cardiac glycosides

1. 4. 1. 4.

2. 5. 2. 5.

3. 3.

(S961125B) (S961126F)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : JL08S041546WT Eluent : acetonitrile/water (35/65) Eluent : acetonitrile/water (35/65) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 220 nm, 0.13 AUFS Detection : UV at 220 nm, 0.13 AUFS Injection : 4 µL (0.05 ~ 0.2 mg/mL) Injection : 4 µL (0.05 ~ 0.2 mg/mL)

Cardiac glycosides Cardiac glycosides

1. Lanatoside C

1. Ouabagenin

2. Proscillaridin A

2. G-Strophanthin(Ouabain)

3. Lanatoside B (S961129G) (S921217A)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 75 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : AS12S05L546WT Part number : AS12S5L546WT Eluent : acetonitrile/water (30/70) Eluent : acetonitrile/water (10/90) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 220 nm, 0.13 AUFS Detection : UV at 220 nm, 0.26 AUFS Injection : 8 µL (0.1 ~ 0.2 mg/mL) Injection : 5 µL (0.2, 0.4 mg/mL)

O Steroids from toad venom 1. O

H3C

H3C OH HO H Bufalin O O 2.

H3C 3 OCCH3 2 H3C O O 1 HO H Cinobufagin

3. O O 4 H3C

H3C O HO H Resibufogenin

4. CO Cl N CH3 H3CO CH2COOH 0 10 20 min Indomethacin (I.S.) @

(S010806A)

Column : YMC-Pack™ ODS-AL™ ( 5µm,120Å) 150 x 4.6 mm I.D. Part number : AL12S051546WT Eluent : acetonitrile/0.1% phosphoric acid (45/55) Flow rate : 1.0 mL/min Temperature : 40 ˚C Pressure : 7.2 MPa Detection : UV at 300 nm, 0.08 AUFS Injection : 10 µL (0.4 ~ 0.08 mg/mL)

Pain-killing drug Indometacin

1. Acetaminophen 1. p-Chlorobenzoic acid 2. Caffeine 2. p-Hydroxybenzoic acid n-butyl ester 3. Ethenzamide 3. Indometacin 4. Bromovalerylurea

(G900727D) (G900727F)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : AA12S051546WT Eluent : acetonitrile/0.1% phosphoric acid (3/17) Eluent : methanol/0.1% phosphoric acid (70/30) Flow rate : 1.0 mL/min Flow rate : 1.3 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.16 AUFS Detection : UV at 254 nm, 0.64 AUFS Injection : 5 µL Injection : 20 µL

A cough/cold medication Ingredients in a cough/cold medication

1. Caffeine 1. Acetaminophen 2. Chlorpheniramine maleate 2. Caffeine 3. Narcotine 3. Chloropheniramine malate 4. Ethenzamide 4. Narcotine 5. Bucetine 5. Ethenzamide 6. Bucetine

(G910523U) (G910523E) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 250 x4.6 mm I.D. Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Part number : AM12S052546WT 250 x 4.6 mm I.D. Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Part number : AM12S052546WT Flow rate : 1.0 mL/min Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Temperature : 30 ˚C Flow rate : 1.0 mL/min Detection : UV at 254 nm, 0.08 AUFS Temperature : 30 ˚C Injection : 3 µL (10 mg/mL) Detection : UV at 254 nm, 0.08 AUFS Sample : an extract from a commercially available cough/cold preparation Injection : 10 µL (0.02 ~ 0.22 mg/mL)

Ingredients in a cough/cold medication Ingredients in a cough/cold medication

1. Acetaminophen 1. Acetaminophen 2. Caffeine 2. Caffeine 3. Chloropheniramine malate 3. Chloropheniramine malate 4. Narcotine 4. Narcotine 5. Ethenzamide 5. Ethenzamide 6. Bucetine 6. Bucetine

(G910523F) (G910523G)

Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 6.0 mm I.D. 150 x 6.0 mm I.D. Part number : AM12S052506WT Part number : AA12S051506WT Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.08 AUFS Detection : UV at 254 nm, 0.08 AUFS Injection : 17 µL (0.02 ~ 0.22 mg/mL) Injection : 17 µL (0.02 ~ 0.22 mg/mL)

Analgesics Analgesics

1. Acetaminophen 1. Acetaminophen

2. Caffeine 2. Caffeine

3. Ethenzamide

4. Apronalide 4. Apronalide

(S930422G) (S921217A)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ C8 (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : OC12S051546WT Eluent : methanol/water (40/60) Eluent : methanol/water (40/60) Flow rate : 0.7 mL/min Flow rate : 0.7 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 220 nm, 0.51 AUFS Detection : UV at 220 nm, 0.51 AUFS Injection : 5 µL (0.2 ~ 2.0 mg/mL) Injection : 5 µL (0.2 ~ 2.0 mg/mL)

Benzethonium chloride Benzalkonium chloride

1. Benzethonium chloride

1. Benzalkonium chloride R=-C12H25

2. Benzalkonium chloride R=-C14H29

Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. (T920527A) Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. (T920529D)

Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 150 x 6.0 mm I.D. 150 x 6.0 mm I.D. Part number : AM12S051506WT Part number : AM12S051506WT

Eluent : acetonitrile/water/NH4SCN (70/30/0.5, v/v/w) Eluent : acetonitrile/water/NH4SCN (70/30/0.5, v/v/w) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 270 nm, 0.16 AUFS Detection : UV at 260 nm, 0.02 AUFS Injection : 10 µL (1.0 mg/mL) Injection : 10 µL (1.0 mg/mL benzalkonium chloride)

Ingredients in a drug for rhinitis Ingredients in an eye drop

(J010418B) (J010406A)

Column : Hydrosphere™ C18 (3µm, 120Å) Column : Hydrosphere™ C18 (3µm, 120Å) 50 x 4.6 mm I.D. 50 x 4.6 mm I.D. Part number : HS12S030546WT Part number : HS12S030546WT

Eluent : A)20mM KH2PO4 - H3PO4 (pH 2.5) Eluent : A)20mM KH2PO4 - H3PO4 (pH 2.5) B)acetonitrile B)acetonitrile 0-40%B(0-10min) 0-60%B(0-10min), 60%B(10-15min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.064 AUFS Detection : UV at 265 nm, 0.128 AUFS Injection : 3 µL (0.02 - 0.5 mg/mL) Injection : 5 µL (0.03 - 0.3 mg/mL)

Pain-killing drugs Codeine

1. Codeine

(A001019B) (J010528A) Column : YMC-Pack™ Pro C4™, Pro C8™ and Pro C18™ (5µm,120Å) 150 x 4.6 mm I.D. Column : YMC-Pack™ Pro C18™ (5µm,120Å) Part number : AS12S051546WT, OS12S051546WT, BS12S051546WT 150 x 4.6 mm I.D.

Eluent : methanol/20mM H3PO4 (60/40) Part number : AS12S051546WT Flow rate : 1.0 mL/min Eluent : acetonitrile/0.05% TFA (10/90) Temperature : 37 ˚C Flow rate : 1.5 mL/min Detection : UV at 254 nm, 0.32 AUFS Detection : UV at 210 nm Injection : 10 µL (0.01 - 2.0 mg/mL) Injection : 5 µL (0.1 mg/mL)

Collunarium Naphazoline hydrochloride and chlorpheniramine maleate

1. Naphazoline hydrochloride

1. Naphazoline hydrochloride 2. Chlorpheniramine maleate

2. Chlorpheniramine maleate

3. Benzethonium chloride

Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. (Y920610A) Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. (T920526A) Column : YMC-Pack™ Phenyl (5µm, 120Å) 150 x 4.6 mm I.D. Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Part number : PH12S051546WT 150 x 6.0 mm I.D.

Eluent : acetonitrile/water/NH4SCN (48/52/0.5, v/v/w) Part number : AM12S051506WT Flow rate : 1.0 mL/min Eluent : acetonitrile/water/NH4SCN (35/65/0.5, v/v/w) Temperature : 30 ˚C Flow rate : 1.0 mL/min Detection : UV at 270 nm, 2.56 AUFS Temperature : 30 ˚C Injection : 10 µL (0.2 ~ 5 mg/mL) Detection : UV at 260 nm, 0.16 AUFS Sample : collunarium Injection : 10 µL (0.125 mg/mL)

Histamine antagonists Histamine antagonists

(J010619A) (A980723A)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ Pro C18™ (3µm, 120Å) 150 x 4.6 mm I.D. 75 x 2.0 mm I.D. Part number : AS12S051546WT Part number : AS12S03L502WT Eluent : 20mM KH PO - K HPO (pH 6.9) / methanol (35/65) 2 4 2 4 Eluent : acetonitrile / 50mM CH3COONH4 (40/60) Flow rate : 1.0 mL/min Flow rate : 0.2 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.13 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 5 µL (0.05 - 2.0 mg/mL) Injection : 2 µL (0.05 - 2.0 mg/mL)

Acidic non-steroidal anti-inflammatory drugs Acidic non-steroidal anti-inflammatory drugs

1. Piroxicam 1. Piroxicam

2. Sulindac

3. Tolmetin sodium dihydrate

4. Ketoprofen

5. Naproxen

5. Naproxen (O950126A) (O950124A)

™ ™ Column : YMC-Pack ODS-AM (5µm, 120Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : AM12S051546WT Part number : JH08S04L546WT Eluent : 1-propanol/water/acetic acid (30/70/1) Eluent : 2-propanol/water/acetic acid (25/75/1) Flow rate : 0.5 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.04 AUFS Detection : UV at 260 nm, 0.04 AUFS Injection : 3 µL (20 µg/mL) Injection : 5 µL (20µg/mL)

Tricyclic antidepressants Tricyclic antidepressants

1. Desipramine hydrochloride

2. Acenaphthene (I.S.)

3. Imipramine hydrochloride

4. Amitriptyline hydrochloride

(A961016B) (S921217A)

Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Column : YMC-Pack™ Pro C18™ (3µm, 120Å) 150 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : AM12S051546WT Part number : AS12S03L546WT Eluent : 20mM KH PO - K HPO (pH 6.9)/methanol (25/75) 2 4 2 4 Eluent : methanol / 20mM CH3COONH4 (75/25) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37˚C Detection : UV at 254 nm, 0.16 AUFS Detection : UV at 254 nm, 0.32 AUFS Injection : 5 µL (0.1 ~ 0.5 mg/mL) Injection : 5 µL (0.1-0.5 mg/mL)

Antipsychotic drugs Antiepileptics

1. Perphenazine

1. Ethosuximide 2. Prochlorperazine maleate

3. Promazine hydrochloride

2. Primidone

4. Fluphenazine hydrochloride

5. Trifluoperazine hydrochloride 3. Phenobarbital sodium salt

6. Ethopropazine hydrochloride

4. Phenytoin

7. (Profenamine hydrochloride)

5. Carbamazepine

(O950627B) 8. Chlorpromazine hydrochloride (S960723A)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 75 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S04L546WT Part number : AS12S051546WT

Eluent : acetonitrile/water/TFA (30/70/0.1) Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/methanol (50/50) Flow rate : 1.0 mL/min Flow rate : 0.7 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.064 AUFS Detection : UV at 215 nm, 0.32 AUFS Injection : 5 µL (0.02 mg/mL) Injection : 8 µL (0.035 ~ 0.7 mg/mL)

Acidic non-steroidal anti-inflammatory drugs Acidic non-steroidal anti-inflammatory drugs

1. Fenoprofen calcium dihydrate 1. Ketoprofen

2. Flurbiprofen 2. Fenoprofen calcium dihydrate

3. Ibuprofen

3. Flurbiprofen

4. Ibuprofen

(O950125C) (A970321A)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 75 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S04L546WT Part number : AA12S051546WT Eluent : methanol/water/acetic acid (65/35/1) Eluent : methanol/water/acetic acid (65/35/1) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.04 AUFS Detection : UV at 260 nm, 0.032 AUFS Injection : 7 µL (160, 16, 820 µg/mL) Injection : 7 µL (6 ~ 820 µg/mL)

Tricyclic antidepressants Tricyclic antidepressants

1. Doxepin hydrochloride

1. Desipramine hydrochloride

2. Desipramine hydrochloride 2. Acenaphthene (I.S.)

3. Imipramine hydrochloride

4. Amitriptyline hydrochloride

(D960724A) (A961015G)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : AS12S051546WT

Eluent : acetonitrile/50mM NH4H2PO4 (30/70) Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9)/methanol (25/75) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 215 nm, 0.13 AUFS Detection : UV at 254 nm, 0.16 AUFS Injection : 2 µL (0.125 mg/mL) Injection : 5 µL (0.1 ~ 0.5 mg/mL)

Calcium antagonists Calcium antagonists

5. Prenylamine lactate 5. Prenylamine lactate 1. Diltiazem HCl 1. Diltiazem HCl

6. Bepridil HCl 2. Verapamil HCl 6. Bepridil HCl 2. Verapamil HCl

3. Methoxyverapamil HCl 7. Niguldipine HCl 3. Methoxyverapamil HCl 7. Niguldipine HCl

4. Nicardipine HCl 4. Nicardipine HCl 8. Nifedipine 8. Nifedipine

(S930702C) (S930701D)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : JM08S041546WT Eluent : 20mM KH PO - H PO (pH 3.0)/acetonitrile/THF/TBA•Br 2 4 3 4 Eluent : 20mM KH2PO4 - H3PO4 (pH 3.0)/acetonitrile/THF/TBA•Br (75/15/10/0.5, v/v/v/w) (70/20/10/0.35, v/v/v/w) Flow rate : 0.8 mL/min Flow rate : 0.8 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 270 nm, 0.064 AUFS Detection : UV at 270 nm, 0.064 AUFS Injection : 8 µL (0.05 ~ 1.4 mg/mL) Injection : 8 µL (0.05 ~ 1.4 mg/mL)Text

Calcium antagonists Antiarrhythmic drugs

5. Prenylamine lactate 1. Diltiazem HCl

6. Bepridil HCl 2. Verapamil HCl

3. Methoxyverapamil HCl 7. Niguldipine HCl

4. Nicardipine HCl 8. Nifedipine

(S930702A) (S990819B) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 150 x 4.6 mm I.D. Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Part number : JL08S041546WT 250 x 4.6 mm I.D.

Eluent : 20mM KH2PO4 - H3PO4 (pH 3.0)/acetonitrile/THF/TBA•Br Part number : AS12S052546WT (70/20/10/0.35, v/v/v/w) Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (30/70) Flow rate : 0.8 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 270 nm, 0.064 AUFS Detection : UV at 220 nm, 0.26 AUFS Injection : 8 µL (0.05 ~ 1.4 mg/mL) Injection : 5 µL (0.05 - 0.1 mg/mL)

Hypolipidemic drugs Hypolipidemic drugs

1. Bezafibrate

2. Gemfibrozil

3. Clofibrate 3. Clofibrate

4. Fenofibrate 4. Fenofibrate

(S930706E) (S930706C)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : JM08S041546WT Eluent : acetonitrile/water/acetic acid(60/40/1) Eluent : acetonitrile/water/acetic acid(60/40/1) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 275 nm, 0.064 AUFS Detection : UV at 275 nm, 0.064 AUFS Injection : 8 µL (0.05~0.25 mg/mL) Injection : 8 µL (0.05 ~ 0.25 mg/mL)

Hypolipidemic drugs Lovastatin

1. Bezafibrate

2. Gemfibrozil

1. Lovastatin

3. Clofibrate

4. Fenofibrate

(S930706B) (J010528B)

Column : J’Sphere™ ODS-L80 (4µm, 80Å) Column : YMC-Pack™ Pro C18™ (5µm,120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JL08S041546WT Part number : AS12S051546WT Eluent : acetonitrile/water/acetic acid(60/40/1) Eluent : acetonitrile/0.05% formic acid (30/70) Flow rate : 1.0 mL/min Flow rate : 1.5 mL/min Temperature : 30 ˚C Detection : UV at 240 nm Detection : UV at 275 nm, 0.064 AUFS Injection : 5 µL (0.1 mg/mL) Injection : 8 µL (0.05 ~ 0.25 mg/mL)

Diuretics Antineoplastic and related compounds

1. Uracil

2. 5-Fluorouracil

3. Deoxyuridine

4. 5-Fluoro-2'-deoxyuridine

(A980716A) (T920924E)

Column : YMC-Pack™ Pro C18™ (3µm, 120Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 75 x 2.0 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S03L502WT Part number : AA12S051546WT Eluent : methanol / 1% acetic acid (40/60) Eluent : 10mM NH4H2PO4 Flow rate : 0.2 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 280 nm, 0.08 AUFS Detection : UV at 254 nm, 0.16 AUFS Injection : 3 µL (0.015 - 0.2 mg/mL) Injection : 10 µL (0.025 ~ 0.2 mg/mL)

Antineoplastic and related compounds Folic acid and its antagonists

1. Uracil

1. Aminopterin

2. 5-Fluorouracil

2. Methotrexate

3. Deoxyuridine 3. Folic acid

4. 5-Fluoro-2'-deoxyuridine

(T920924G) (S921217A)

Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : AQ12S051546WT Part number : JM08S04L546WT

Eluent : 10mM NH4H2PO4 Eluent : methanol/water/acetic acid (20/80/1) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : ambient(25 ˚C) Detection : UV at 254 nm, 0.16 AUFS Detection : UV at 300 nm, 0.04 AUFS Injection : 10 µL (0.025 ~ 0.2 mg/mL) Injection : 5 µL (16 µg/mL)

9 Drugs and Metabolites Antiarrhythmic drugs Local anesthetics 1. O H2N COCH2CH3

Benzocaine

(Ethyl 4-aminobenzoate) á 2. O

H2N COCH2CH2N(CH2CH3)2 áHCl Procaine HCl

3. CH2 CH H

N HO C H H

N 1 Cinchonine 2 4. á CH3 O NHCCH2N(CH2CH3)2 CH3 5 Lidocaine

5. O 4 COCH2CH2N(CH3)2

3 áHCl á 6 NHCH2CH2CH2CH3

Tetracaine HCl 6. O á H2N COCH2CH2CH2N(C4H9)2

Butacaine

(S990817C) 0 5 10 15 min (J010302E)

Column : YMC-Pack™ Pro C4™ (5µm, 120Å) Column : Hydrosphere™ C18 (3µm, 120Å) 150 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : BS12S051546WT Part number : HS12S03L546WT

Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (40/60) Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (40/60) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 220 nm, 0.26 AUFS Detection : UV at 254 nm, 0.16 AUFS Injection : 5 µL (0.05 - 0.1 mg/mL Injection : 10 µL (0.02 - 0.5 mg/mL)

Diuretics Diuretics

1. Hydrochlorothiazide 1. Hydrochlorothiazide

2. Chlorthalidone 2. Chlorthalidone

3. Furosemide 3. Furosemide

(A970425C) (A970426D)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : AQ12S051546WT

Eluent : 20mM NH4H2PO4 - H3PO4(pH 3.2)/methanol (60/40) Eluent : 20mM NH4H2PO4 - H3PO4(pH 3.2)/methanol (60/40) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 280 nm, 0.16 AUFS Detection : UV at 280 nm, 0.16 AUFS Injection : 15 µL (0.015 ~ 0.2mg/mL) Injection : 15 µL (0.015 ~ 0.2mg/mL)

Nicotinic acid analogues Basic compounds

1. Isonicotinic acid 1. Uracil

2. Procainamide 2. Nicotinic acid

3. N-Acetylprocainamide

3. Isonicotinic acid hydrazide

4. 4'-Hydroxyacetophenone

4. Nicotinamide

5. N' -Methylnicotinamide

(K940125K) (S921217A)

Column : YMC-Pack™ C8 (5µm, 120Å) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : OC12S051546WT Part number : AM12S051546WT Eluent : acetonitrile/20mM KH PO (5/95) 2 4 Eluent : 20mM K2HPO4 - KH2PO4 (pH 6.9)/acetonitrile (92/8) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.64 AUFS Detection : UV at 254 nm, 0.32 AUFS Injection : 13 µL (0.2 mg/mL) Injection : 10 µL (30 ~ 300 É g/mL)

Basic compounds Disinfectants

1. Benzoic acid 1. Uracil

2. Procainamide 2. Salicylic acid

3. N-Acetylprocainamide

3. Theophylline (I.S.)

4. 4'-Hydroxyacetophenone

4. Phenol

(T931217B) (S930617D)

Column : YMC-Pack™ PolymerC18™ Column : YMC-Pack™ ODS-AL™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : PC99S061546WT Part number : AL12S051546WT

Eluent : 20mM K2HPO4 - KH2PO4 (pH 6.9)/acetonitrile (92/8) Eluent : 100mM KH2PO4 - Na2HPO4 (pH 7.0)/methanol (75/25) Flow rate : 1.0 mL/min Flow rate : 0.8 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.32 AUFS Detection : UV at 270 nm, 0.13 AUFS Injection : 10 µL (30 ~ 300 É g/mL) Injection : 3 µL (0.1 ~ 0.8 mg/mL)

Acyclovir Carnitine chloride

1. + - (CH3)3N CH2CHCH2COOH Cl 2 1 OH

Carnitine chloride

2. HN N

CH2CHCOOHáHCl 1. Acyclovir NH2 L-Histidine hydrochloride

0 5 10 15 20 min

(F000629A) (J010528C) Column : Hydrosphere™ C18 (5µm, 120Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 150 X 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : HS12S051546WT

Part number : AS12S051546WT Eluent : 50mM NaH2PO4-H3PO4(pH2.6) Eluent : acetonitrile / 0.05% formic acid (3 / 97) containing 15mM 1-pentanesulfonic acid sodium salt Flow rate : 1.5 mL/min Flow rate : 1.0 mL/min Detection : UV at 254 nm Temperature : 30 ˚C Injection : 5 µL (0.1 mg/mL) Detection : UV at 210 nm, 0.064 AUFS Injection : 4 µL (0.2 ~ 20 mg/mL)

Xanthine oxidase inhibitor and related compounds Xanthine oxidase inhibitor and related compounds

1. Uric acid 1. Uric acid

2. Hypoxanthine 2. Hypoxanthine

3. Xanthine 3. Xanthine

4. Oxipurinol 4. Oxipurinol

5. Allopurinol 5. Allopurinol

(S930728J) (S930729B)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JM08S041546WT Part number : JL08S041546WT

Eluent : 50mM NH4H2PO4 Eluent : 50mM NH4H2PO4 Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.51 AUFS Detection : UV at 260 nm, 0.51 AUFS Injection : 8 µL (0.05 ~ 0.2 mg/mL) Injection : 8 µL (0.05 ~ 0.2 mg/mL)

Anti-human immunodeficiency virus (HIV) agents Anti-human immunodeficiency virus (HIV) agents

1. DDC 1. DDC

2. DDI 2. DDI

3. D4T 3. D4T

4. AZT 4. AZT (K930311C) (K930311A)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : YMC-Pack™ C8 (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S051546WT Part number : OC12S051546WT

Eluent : methanol/10mM KH2PO4 (10/60) Eluent : methanol/10mM KH2PO4 (10/60) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.16 AUFS Detection : UV at 254 nm, 0.16 AUFS Injection : 7 µL (0.125 mg/mL) Injection : 7 µL (0.125 mg/mL)

Anti-human immunodeficiency virus (HIV) agents Nicotinic acid analogues

1. Isonicotinic acid

1. DDC 2. Nicotinic acid

2. DDI 3. Isonicotinic acid hydrazide

4. Nicotinamide 3. D4T

5. N' -Methylnicotinamide 4. AZT (K930311F) (K940125I)

Column : YMC-Pack™ C4 (5µm, 120Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : BU12S051546WT Part number : AA12S051546WT

Eluent : methanol/10mM KH2PO4 (10/60) Eluent : acetonitrile/20mM KH2PO4 (5/95) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.16 AUFS Detection : UV at 260 nm, 0.64 AUFS Injection : 7 µL (0.125 mg/mL Injection : 13 µL (0.2 mg/mL)

Hippuric and methylhippuric acids Acidic and basic drugs 1. CH3 CHCOOH

F 1. Mandelic acid Flurbiprofen

2. COOCH3 Methyl benzoate

2. Hippuric acid 3. CH3 O NHCCH N(CH CH ) 2 2 2 3 2 CH3 Lidocaine

4. OCH3 1 3. o-Methylhippuric acid ·HCl S OCOCH3 N 3 O 4 CH2CH2N(CH3)2 Diltiazem HCl 5. 4. m-Methylhippuric acid H CO OCH 5 3 CN CH3 3 H3CO C (CH2)3NCH2CH2 OCH3 CH(CH3)2 ·HCl Verapamil HCl

5. p-Methylhippuric acid 0 10 20 30 min

(A961119A) (C020517A)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : YMC-Pack™ Pro C18 RS™ (5µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JM08S041546WT Part number : RS08S051546WT

Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.2)/methanol (80/20) Eluent : 20mM CH3COONH4(ph 6.8) / methanol (40/60) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 225 nm, 0.16 AUFS Detection : UV at 220 nm, 0.64 AUFS Injection : 10 µL (0.04 ~ 0.07 mg/mL) Injection : 10 µL (0.1mg/mL, 0.036 mg/mL)

Acidic and basic drugs Chlorhexidine

1. CH3 CHCOOH 1. NH NH NH NH F 2 Flurbiprofen Cl NHCNHCNH(CH2)6NHCNHCNH Cl

2. E2HCl COOCH3 Chlorhexidine E2HCl Methyl benzoate 1 1 3. CH3 O NHCCH2N(CH2CH3)2 CH3 2. COOCH3 Lidocaine 2 4. 3 OCH3 ·HCl Methyl benzoate 4 S OCOCH3 N O CH2CH2N(CH3)2 Diltiazem HCl 5. H CO OCH 5 3 CN CH3 3 H3CO C (CH2)3NCH2CH2 OCH3 CH(CH3)2 ·HCl Verapamil HCl

0 10 20 30 min (C020425A) 0 10 20 min (A020117C)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JM08S041546WT Part number : AS12S051546WT

Eluent : 20mM CH3COONH4-NH3 (pH8.9) /methanol (35/65) Eluent : acetonitrile/water/TFA (30/70/0.1) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Pressure : 13.1 MPa Pressure : 8.0 MPa Detection : UV at 220 nm, 0.64 AUFS Detection : UV at 220 nm, 0.16 AUFS Injection : 10 µL (0.1 mg/mL, 0.036µL/mL) Injection : 10 µL (0.1mg/mL )

Purine catabolites Purine catabolites

1. Allantoin 1. Allantoin 2. Allantoic acid 2. Allantoic acid 3. Uric acid 3. Uric acid

(G910607G) (G910618A)

Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) 150 x 6.0 mm I.D. 150 x 6.0 mm I.D. Part number : AM12S051506WT Part number : AQ12S051506WT

Eluent : 10mM CH3COOH Eluent : 10mM CH3COOH Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 200 nm, 0.16 AUFS Detection : UV at 200 nm, 0.16 AUFS Injection : 4 µL (0.10 ~ 0.71 mg/mL) Injection : 4 µL (0.10 ~ 0.71 mg/mL)

Catecholamines Hippuric and methylhippuric acids

1. Norepinephrine hydrochloride 2. Epinephrine 1. Mandelic acid 3. 3,4-Dihydroxyphenylalanine 1 (DOPA) 4. Dopamine hydrochloride 5. 4-Hydroxy-3-methoxymandelic acid 6. 3-Methoxytyramine hydrochloride 2. Hippuric acid 7. 4-Hydroxy-3-methoxyphenylglycol 8. 3,4-Dihydroxyphenylacetic acid 3 9. Homovanilic acid

2 4 3. o-Methylhippuric acid

6 8 7 5 9

4. m-Methylhippuric acid

0 10 20 30 40 min 5. p-Methylhippuric acid

(F000817A)

Column : Hydrosphere™ C18 (5µm, 120Å) (A961114A) 150 x 4.6 mm I.D. Part number : HS12S051546WT Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 150 x 4.6 mm I.D. Eluent : A)50mM KH2PO4-H3PO4(pH 3.1) Part number : AS12S051546WT B)50mM KH2PO4-H3PO4(pH 3.1)/acetonitrile (90/10) 0%B (0-5min), 0-100%B (5-25min,linear), 100%B (25-35min) Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.2)/methanol (80/20) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 280 nm, 0.64 AUFS Detection : UV at 225 nm, 0.16 AUFS Injection : 10 µL (0.15 - 0.25 mg/mL) Injection : 10 µL (0.04 ~ 0.07 mg/mL)

Sulfonamides Sulfonamides 1. Sulfamethazine

1. Sulfaguanidine 6. Sulfathiazole

2. Sulfadoxine 2. Sulfamethizole 7. Trimethoprim

3. Sulfamonomethoxine hydrate 3. Sulfanilamide 8. Sulfapyridine

4. Sulfisomidine 9. Sulfamerazine 4. Sulfisoxazole

5. Sulfadiazine 10. Sulfamethoxypyridazine 5. Sulfamethoxazole

11. Sulfamethazine 6. Sulfachloropyridazine

7. Sulfadimethoxine

8. Sulfaquinoxaline sodium (O950324L) (O950302C)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : YMC-Pack™ ODS-A™ (5µm, 120Å) 250 x4.6 mm I.D. 250 x 4.6 mm I.D. Part number : JH08S042546WT Part number : AA12S052546WT

Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.2)/THF (90/10) Eluent : THF/water/acetic acid (30/70/1) Flow rate : 0.5 mL/min Flow rate : 0.5 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 275 nm, 0.16 AUFS Detection : UV at 275 nm, 0.16 AUFS Injection : 10 µL (18 µg/mL) Injection : 5 µL (42 µg/mL)

Sulfonamides Sulfonamides

1. CH3 5. N N NH2 SO2NH N NH2 SO2NH N

CH3 OCH3 1. Sulfisomidine 6. Sulfamonomethoxine hydrate Sulfisomidine Sulfamonomethoxine

2. 6. S NH2 SO2NH NH2 SO2NH 7. Sulfadoxine 1 N N 2. Sulfathiazole O CH3 Sulfathiazole Sulfamethoxazole

3. 7. OCH3 3. Sulfadiazine N N 8. Sulfamethoxazole NH2 SO2NH NH2 SO2NH N N CH3 OCH3 2 Sulfamerazine Sulfadimethoxine

3 4. Sulfamerazine 9. Sulfadimethoxine 4. CH3 N NH2 SO2NH N CH3 4 Sulfadimidine

5. Sulfamethazine 10. Sulfaquinoxaline sodium 5 7

0 5 10 15 20 min (J010321F) (O950217D) Column : Hydrosphere™ C18 (3µm, 120Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 50 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : HS12S030546WT

Part number : JM08S04L546WT Eluent : 10mM CH3COOH - CH3COONH4 (pH 4.6) / acetonitrile

Eluent : THF/acetonitrile/50mM NH4H2PO4 (15/5/80) (85/15) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 275 nm, 0.16 AUFS Detection : UV at 275 nm, 0.16 AUFS Injection : 3 µL (50 µg/mL) Injection : 10 µL (0.01 - 0.04 mg/mL)

Antibacterial agents Antibacterial agents

1. Olaquindox 1. Olaquindox 2. Carbadox 2. Carbadox 3. Thiamphenicol 3. Thiamphenicol 4. Sulfamerazine 4. Sulfamerazine 5. Sulfadimidine 5. Sulfadimidine 6. Sulfamonomethoxine 6. Sulfamonomethoxine 7. Furazolidone 7. Furazolidone 8. Oxolinic acid 8. Oxolinic acid 9. Sulfaquinoxaline 9. Sulfaquinoxaline 10. Sulfadimethoxine 10. Sulfadimethoxine 11. Nalidixic acid 11. Nalidixic acid 12. Piromidic acid 12. Piromidic acid

(K940614B) (K940615C)

™ ™ Column : YMC-Pack ODS-A (5µm, 120Å) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : AA12S052546WT Part number : JL08S042546WT Eluent : A)acetonitrile/50mM NH H PO (10/90) 4 2 4 Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90) B)acetonitrile/50mM NH H PO (80/20) 4 2 4 B)acetonitrile/50mM NH4H2PO4 (80/20) 25%B (0-5min), 25-100%B (5-15min), 100%B (15-20min) 25%B (0-5min), 25-100%B (5-15min), 100%B (15-20min) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 240 nm, 0.32 AUFS Detection : UV at 240 nm, 0.32 AUFS Injection : 10 µL (0.05 mg/mL Injection : 10 µL (0.05 mg/mL)

Antibacterial agents Sulfonamides

1. Sulfaguanidine 6. Sulfapyridine

1. Diaveridine 2. Sulfanilamide 7. Sulfamerazine

3. Sulfisomidine 2. Trimethoprim 8. Sulfamethizole

4. Sulfathiazole 9. Sulfamethoxypyridazine

3. Pyrimethamine 5. Sulfadiazine 10. Sulfamethazine

(O950320G) (O950306E)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 75 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : JM08S04L546WT Part number : JM08S042546WT

Eluent : acetonitrile/50mM NH4H2PO4 (15/85) Eluent : THF/50mM NH4H2PO4 (10/90) Flow rate : 1.0 mL/min Flow rate : 0.5 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 230 nm, 0.16 AUFS Detection : UV at 275 nm, 0.16 AUFS Injection : 5 µL (20 µg/mL) Injection : 3 µL (50 µg/mL)

Sulfonamides Antibiotics

1. 5. N N NH2 SO2NH NH2 SO2NH N N OCH3 1. Ampicillin Sulfadiazine Sulfamonomethoxine 2. Cephalexin 3. Oxytetracycline hydrochloride 4. Tetracycline 2. 6. 1 S 5. Chlorotetracycline NH2 SO2NH NH2 SO2NH N N O CH3 6. Chloramphenichol Sulfathiazole Sulfamethoxazole 7. Oxolinic acid 2 8. Doxorubicin hydrochloride 9. Nalidixic acid 3. N 3 NH2 SO2NH N CH3 Sulfamerazine

4. CH3 N NH2 SO2NH 5 N CH3 Sulfadimidine 6

(G910523M) 0 5 10 min (J010130D) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) Column : Hydrosphere™ C18 (3µm, 120Å) 150 x 4.6 mm I.D. 75 x 2.0 mm I.D. Part number : AM12S051546WT Part number : HS12S03L502WT Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90) Eluent : 10mM CH COOH - CH COONH (pH 4.6) / acetonitrile 3 3 4 B)acetonitrile/50mM NH4H2PO4 (50/50) (85/15) 0-55%B (0-30min,linear), 55%B (30-40min) Flow rate : 0.2 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 275 nm, 0.16 AUFS Detection : UV at 250 nm, 0.16 AUFS Injection : 2 µL (0.02 mg/mL) Injection : 15 µL (0.01 ~ 0.15 mg/mL)

Nalidixic acid antibiotics Nalidixic acid antibiotics 1. Pipemidic acid 1. Pipemidic acid

2. Enoxacin 2. Enoxacin

3. Cinoxacin 3. Cinoxacin

4. Norfloxacin 4. Norfloxacin

5. Ofloxacin

6. Nalidixic acid

7. Oxolinic acid

7. Oxolinic acid 8. Piromidic acid

(O950428A) (O950428D) 8. Piromidic acid ™ ™ Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : YMC-Pack ODS-AM (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : AM12S051546WT Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/tetrahydrofuran (90/10) Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/tetrahydrofuran (90/10) containing 20mM TBA•Br containing 20mM TBA•Br Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (25 ˚C) Temperature : ambient (25 ˚C) Detection : UV at 260 nm, 0.32 AUFS Detection : UV at 260 nm, 0.32 AUFS Injection : 5 µL (0.03 mg/mL) Injection : 5 µL (0.03 mg/mL)

Nalidixic acid antibiotics Nalidixic acid antibiotics

(A010324C) (A010324A)

™ Column : Hydrosphere™ C18 (3µm, 120Å) Column : Hydrosphere C18 (3µm, 120Å) 50 x 2.0 mm I.D. 50 x2.0 mm I.D. Part number : HS12S030502WT Part number : HS12S030502WT Eluent : 10mM CH3COOH - CH3COONH4(pH 4.3) / acetonitrile Eluent : acetonitrile / 50mM NaH2PO4 (25/75) Flow rate : 0.2 mL/min (75/25) Temperature : 37 ˚C Flow rate : 0.2 mL/min Detection : UV at 260 nm, 0.128 AUFS Temperature : 37 ˚C Injection : 1 µL (0.02 - 0.1 mg/mL) Detection : UV at 260 nm, 0.128 AUFS Injection : 1 µL (0.02 - 0.1 mg/mL)

Cephalosporin antibiotics Cephalosporin antibiotics

1. Cefsulodin sodium 1. Cefsulodin sodium 2. Cephalexin hydrate 2. Cephalexin hydrate 3. Cefaclor 3. Cefaclor 4. Cephradine 4. Cephradine 5. Cephaloglycin 5. Cephaloglycin 6. Cephapirin sodium 6. Cephapirin sodium 7. Cefotaxime sodium 7. Cefotaxime sodium 8. Cephaloridine 8. Cephaloridine 9. Cefazolin sodium 9. Cefazolin sodium 10. Cefuroxime sodium 10. Cefuroxime sodium 11. Cefoxitin sodium 11. Cefoxitin sodium

(O940719A) (O940715F)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : JH08S042546WT Part number : JM08S042546WT

Eluent : acetonitrile/100mM KH2PO4 (10/90) Eluent : acetonitrile/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Flow rate : 0.8 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 5 µL (0.12 ~ 0.35 mg/mL) Injection : 5 µL (0.12 ~ 0.35 mg/mL)

Cephalosporin antibiotics Cephalosporin antibiotics

1. Cefadroxil 1. Cefadroxil

2. Cephalosporin C potassium 2. Cephalosporin C potassium

3. Cefatrizine propylene glycol 3. Cefatrizine propylene glycol

4. Cephalexin hydrate 4. Cephalexin hydrate

5. Cephaloridine 5. Cephaloridine (O940927G) (O941025E)

Column : YMC-Pack™ ODS-AQ™ (5µm, 120Å) Column : YMC-Pack™ ODS-AM™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AQ12S051546WT Part number : AM12S051546WT Eluent : methanol/water/acetic acid (10/85/5) Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 6 µL (0.2 mg/mL) Injection : 6 µL (0.2 mg/mL)

Cephalosporin antibiotics Cephalosporin antibiotics

1. Cefoperazone sodium 1. Cefoperazone sodium

2. Cephalothin sodium 2. Cephalothin sodium

3. Cefamandole sodium 3. Cefamandole sodium

(O940928P) (O940928Q)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 75 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : JH08S04L546WT Part number : JM08S04L546WT

Eluent : THF/50mM NH4H2PO4 (15/85) Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 6 µL (0.17 mg/mL) Injection : 6 µL (0.17 mg/mL)

Cephalosporin antibiotics Cephalosporin antibiotics

1. Cefsulodin sodium 2. Cephalexin hydrate 3. Cefaclor 4. Cephradine 1. Cefadroxil 5. Cephaloglycin 6. Cephapirin sodium 7. Cefotaxime sodium 8. Cephaloridine 9. Cefazolin sodium 2. Cephalosporin C potassium 10. Cefuroxime sodium 11. Cefoxitin sodium

3. Cefatrizine propylene glycol

4. Cephalexin hydrate

5. Cephaloridine

(O940718B) (O940927B)

™ Column : J’Sphere ODS-L80 (4µ m, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 250 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JL08S042546WT Part number : JH08S041546WT Eluent : acetonitrile/100mM KH2PO4 (10/90) Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 0.8 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 5 µL (0.12 ~ 0.35 mg/mL) Injection : 6 µL (0.2 mg/mL)

Cephalosporin antibiotics Cephalosporin antibiotics

1. Cefadroxil 1. Cefadroxil

2. Cephalosporin C potassium 2. Cephalosporin C potassium

3. Cefatrizine propylene glycol 3. Cefatrizine propylene glycol

4. Cephalexin hydrate 4. Cephalexin hydrate

5. Cephaloridine 5. Cephaloridine

(O940927C) (O940927I)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JM08S04156WT Part number : JL08S041546WT Eluent : methanol/water/acetic acid (10/85/5) Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 6 µL (0.2 mg/mL) Injection : 6 µL (0.2 mg/mL)

Cephalosporin antibiotics Cephalosporin antibiotics

1. Ceftriaxone sodium 1. Ceftriaxone sodium

2. Cefotaxime sodium

(O940928F) (O940929K)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 75 x 4.6 mm I.D. 75 x 2.0 mm I.D. Part number : JM08S04L546WT Part number : JH08S04L502WT 2 Eluent : methanol/50mM NH4H2PO4 (30/70) Eluent : methanol/50mM NH4H PO4 (30/70) Flow rate : 1.0 mL/min Flow rate : 0.2 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 5 µL (0.08, 0.16 mg/mL) Injection : 3 µL (0.08, 0.16 mg/mL)

Cephalosporin antibiotics Cephalosporin antibiotics

1 S CH CO NH

NH2 O N CH3 COOH 1 Cephalexin 1. Cephaloglycin 2 S 2 CH CO NH 3 NH2 O N Cl COOH Cefaclor

3 S CH CO NH 2. Cefotaxime sodium NH2 O N CH2OCOCH3 4 COOH Cephaloglycin

CO S S CH2 NH + O N CH2 N COOH Cephaloridine 3. Cephapirin sodium

0 5 10 15 20 min

(O940720A) (S000717B)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : Hydrosphere™ C18 (5µm, 120Å) 75 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JM08S04L546WT Part number : HS12S051546WT

Eluent : acetonitrile/100mM KH2PO4 (10/90) Eluent : acetonitrile/20mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.64 AUFS Detection : UV at 260 nm, 0.32 AUFS Injection : 5 µL (0.5 mg/mL) Injection : 15 µL (0.1 ~ 0.2 mg/mL)

Cephalosporin antibiotics Cephalosporin antibiotics

1. Cefoperazone sodium 1. Cefoperazone sodium

2. Cephalothin sodium 2. Cephalothin sodium

3. Cefamandole sodium 3. Cefamandole sodium

(O940928R) (O940929D)

Column : J’Sphere™ ODS-L80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 75 x 4.6 mm I.D. 75 x 2.0 mm I.D. Part number : JL08S04L546WT Part number : JH08S04L502WT

Eluent : THF/50mM NH4H2PO4 (15/85) Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Flow rate : 0.2 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 6 µL (0.17 mg/mL) Injection : 3 µL (0.17 mg/mL)

Cephalosporin antibiotics Cephalosporin antibiotics

1. Ceftriaxone sodium

1. Cefoperazone sodium

2. Cefotaxime sodium

2. Cephalothin sodium

3. Cefamandole sodium

(O940928S) (O940928G)

Column : YMC-Pack™ ODS-A™ (3µm, 120Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 50 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : AA12S030546WT Part number : JH08S04L546WT

Eluent : THF/50mM NH4H2PO4 (15/85) Eluent : methanol/50mM NH4H2PO4 (30/70) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 6 µL (0.17 mg/mL) Injection : 5 µL (0.08, 0.16 mg/mL)

Cefoxitin sodium Penicillin antibiotics 1. Bacampicillin hydrocloride

2. Methicillin sodium

1. Caffeine (I.S.) 3. Penicillin G potassium (Benzylpenicillin potassium)

4. Penicillin V potassium (Phenoxymethylpenicillin potassium)

2. Cefoxitin sodium 5. Phenethicillin potassium (Phenoxyethylpenicillin potassium)

6. Cloxacillin sodium

7. Propicillin potassium

8. Dicloxacillin sodium (O940729A) (O950908A)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 2.0 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041502WT Part number : JH08S041546WT Eluent : 50mM NaH PO - H PO (pH 2.3)/acetonitrile (67/33) Eluent : acetonitrile/50mM KH2PO4 (20/80) 2 4 3 4 Flow rate : 0.34 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.32 AUFS Detection : UV at 220 nm, 0.26 AUFS Injection : 1.2 µL (1.6, 2.4 mg/mL) Injection : 8 µL (0.13 mg/mL)

Penicillin antibiotics Amoxicillin

1. CH HO CH CO NH S 3 CH3 NH N COOH 1. Amoxicillin hydrate 2 O Amoxicillin

1 2. O CH3 H C N 2 3 N O N N 2. Ampicillin anhydrous CH3 Caffeine

0 5 10 15 min (O950920C) (S000622D) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 75 x 4.6 mm I.D. Column : Hydrosphere™ C18 (5µm, 120Å) Part number : JH08S04L546WT 150 x 4.6 mm I.D.

Eluent : 50mM NaH2PO4 - H3PO4 (pH 2.3)/acetonitrile (80/20) Part number : HS12S051546WT containing 20mM sodium 1-heptanesulfonate Eluent : acetonitrile / water / TFA (10/90/0.1) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 220 nm, 0.13 AUFS Detection : UV at 220 nm, 0.32 AUFS Injection : 2 µL (0.3, 0.7 mg/mL) Injection : 10 µL (0.2, 0.1 mg/mL)

Cephalosporin C Ceftriaxone

1. H N S 2 CH (CH ) OCNH 1 HOOC 2 3 1. N CH O COCH O 2 3 N COOK H NHS S C N OH Cephalosporin C potassium 1 2 C CO NH 3 N NOCH 3 O N CH2S N O 2. OH COONa Ceftriaxone sodium OH Resorcinol 2 2. OH

2 Phenol (I.S.)

0 5 10 15 min 0 5 10 15 min

(S000714B) (S000726B)

Column : Hydrosphere™ C18 (5µm, 120Å) Column : Hydrosphere™ C18 (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : HS12S051546WT Part number : HS12S051546WT

Eluent : acetonitrile/20mM KH2PO4 (5/95) Eluent : methanol / 10mM H3PO4 (25/75) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.16 AUFS Detection : UV at 260 nm, 0.32 AUFS Injection : 10 µL (0.2 mg/mL) Injection : 10 µL (0.1, 0.4 mg/mL)

Cefoxitin sodium Cefoxitin sodium

1. Caffeine (I.S.) 1. Caffeine (I.S.)

2. Cefoxitin sodium 2. Cefoxitin sodium

(O940728M) (O940728P)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 75 x 4.6 mm I.D. 75 x 2.0 mm I.D. Part number : JH08S04L546WT Part number : JH08S04L502WT

Eluent : acetonitrile/50mM KH2PO4 (20/80) Eluent : acetonitrile/50mM KH2PO4 (20/80) Flow rate : 1.8 mL/min Flow rate : 0.34 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 260 nm, 0.32 AUFS Detection : UV at 260 nm, 0.32 AUFS Injection : 1.2 µL (8, 12 mg/mL) Injection : 1.2 µL (1.6, 2.4 mg/mL)

Erythromycin antibiotic drugs Tetracycline antibiotics

1. Erythromycin 2. Erythromycin ethylsuccinate 3. Erythromycin estolate

1. Oxytetracycline hydrochloride

2. Tetracycline hydrochloride

(S930910B) (S970524D)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : AS12S051546WT

Eluent : acetonitrile/methanol/100mM (NH4)2HPO4(45/15/40) Eluent : acetonitrile/10mM oxalic acid (10/90) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 45 ˚C Temperature : 37 ˚C Detection : UV at 210 nm, 0.26 AUFS Detection : UV at 280 nm, 0.13 AUFS Injection : 15 µL (2.5 mg/mL) Injection : 6 µL (0.2 mg/mL)

Tetracycline antibiotics Vancomycin 1. OH

H O N OH CO2H OH HO NH O HN O Cl O 1. Oxytetracycline hydrochloride 2 O N O H CNH CH2OH 2 O HN O O O H H OH H HO H Cl NH CH3 H OH NH H O O 1 O H3C H CH 3 HCl CH CH3 2. Tetracycline hydrochloride HO 3 H NH2 Vancomycin hydrochloride

2. O CH C 3

OH 4'-Hydroxyacetophenone

0 10 20 min (S921217A) (S000728B)

™ Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : Hydrosphere C18 (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : HS12S051546WT Eluent : acetonitrile/10mM oxalic acid (10/90) Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / acetonitrile Flow rate : 1.0 mL/min (88/12) Temperature : 37 ˚C Flow rate : 1.0 mL/min Detection : UV at 280 nm, 0.13 AUFS Temperature : 37 ˚C Injection : 4 µL (0.2 mg/mL) Detection : UV at 220 nm, 0.32 AUFS Injection : 10 µL (0.1 mg/mL

Copper 8-quinolinolate Herbicides

1. Copper 8-quinolinolate 1. Atrazine-desisopropyl 2. Atrazine-dsethyl 3. Crimidine 4. Hexazinone 5. Metoxuron 6. Simazine 7. Cyanazine 8. Atraton 9. Atrazine 10 Isoproturon 11. Diuron 12. Prometon 13. Metobromuron 14. Terbumeton 15. Metazachlor 16. Ametryn 17. Sebuthylazine 18. Propazine 19. Terbuthylazine 20. Linuron 21. Prometryn 22. Terbutryn 23. Metolachlor

(O941020H)

(A010717A) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 250 x 4.6 mm I.D. Column : Hydrosphere™ C18 (5µm, 120Å) Part number :JH08S042546WT

150 x 2.0 mm I.D. Eluent : A)acetonitrile/50mM NH4H2PO4 (20/80)

Part number : HS12S051502WT B)acetonitrile/50mM NH4H2PO4 (65/35) 0-100%B (0-60min, linear) Eluent : acetonitrile/20mM H3PO4 (5/95) Flow rate : 0.2 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 250 nm, 0.32 AUFS Detection : UV at 230 nm, 0.16 AUFS Injection : 2 µL (0.02 mg/mL) Injection : 5 µL (0.04 mg/mL)

Atrazine derivatives Triazine herbicides

1. Cyanazine 2. Simazine 3. Atraton 4. Atrazine 1. Atrazine-2-hydroxy 5. Prometon 6. Terbumeton 7. Sebuthylazine 8. Propazine 9. Terbuthylazine 2. Atrazine-dsisopropyl 10. Prometryn 11. Terbutryn

3. Atrazine-deethyl

4. Atrazine

(O941020H) (O941102F)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 75 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S04L546WT Part number : JH08S041546WT

Eluent : acetonitrile/water/TFA (25/75/0.1) Eluent : acetonitrile/50mM NH4H2PO4 (45/55) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 230 nm, 0.16 AUFS Detection : UV at 230 nm, 0.32 AUFS Injection : 5 µL (0.04 mg/mL) Injection : 5 µL (0.05 mg/mL)

Triazine herbicides Urea herbicides

1. Simazine 5. Sebuthylazine 1. Metoxuron

2. Atrazine 6. Propazine 2. Isoproturon

3. Prometon 7. Terbuthylazine

3. Diuron

4. Terbumeton 8. Prometryn 4. Metobromuron

9. Terbutryn 5. Linuron (A970210B) (O941028B)

Column : YMC-Pack™ Pro C18™ (5µm, 120Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 250 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : AS12S052546WT Part number : JH08S04L546WT

Eluent : acetonitrile/50mM NH4H2PO4 (45/55) Eluent : THF/water (30/70) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 230 nm, 0.32 AUFS Detection : UV at 260 nm, 0.16 AUFS Injection : 5 µL (0.05 mg/mL) Injection : 4 µL (0.2 mg/mL)

Acid amide herbicides Carbamate herbicides

1. Propham

1. Metazachlor

2. Swep

2. Metolachlor

3. Chloropropham

3. Alachlor

4. Butachlor

(O941108C) (O941124G)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-L80 (4µm, 80Å) 75 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : JM08S04L546WT Part number : JL08S04L546WT Eluent : 2-propanol/THF/water (25/15/60) Eluent : acetonitrile/water (45/55) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 210 nm, 0.16 AUFS Detection : UV at 240 nm, 0.16 AUFS Injection : 8 µL (0.05 mg/mL)Text Injection : 5 µL (0.08 mg/mL)

Carbamate insecticides Fungicides

O 1. O

(CH3)2NC C NOCNHCH3 SCH3 Oxamyl

1. Thiabendazole 2. O

CH3S C(CH3)2CH NOCNHCH3 2 Aldicarb 1

3. O OCNHCH3 O CH 3 3 2. o-Phenylphenol O CH3 Bendiocarb

4. O 5 OCNHCH3 CH2SC2H5 4 Ethiofencarb 3. Imazalil

5. O N(CH3)2 N (CH3)2NCO N

H3C CH3 Pirimicarb

0 5 10 min 4. Diphenyl

(J010220D) (A970217A)

Column : Hydrosphere™ C18 (3µm, 120Å) Column : YMC-Pack™ Pro C18™ (5µm, 120Å) 75 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : HS12S03L546WT Part number : AS12S05L546WT Eluent : methanol/20mM NH H PO (70/30) Eluent : methanol / 20mM KH2PO4 (45/55) 4 2 4 Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 37 ˚C Detection : UV at 240 nm, 0.64 AUFS Detection : UV at 230 nm, 0.32 AUFS Injection : 5 µL (0.1 - 1.5 mg/mL) Injection : 10 µL (0.02 ~ 0.20 mg/mL)

Fungicides Imazalil

1. Imazalil

1. Thiabendazole

2. o-Phenylphenol

3. Imazalil

4. Diphenyl

(A970219A) (B931007A)

Column : YMC-Pack™ ODS-A™ (5µm, 120Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 75 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AA12S05L546WT Part number : JM08S041546WT

Eluent : methanol/20mM NH4H2PO4 (70/30) Eluent : acetonitrile/20mM NH4H2PO4 (50/50) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 230 nm, 0.32 AUFS Detection : UV at 230 nm, 0.04 AUFS Injection : 10 µL (0.02 ~ 0.20 mg/mL) Injection : 10 µL (0.05 mg/mL)

2,4-Dinitrophenylhydrazones of aldehydes and ketones 2,4-dinitrophenylhydrazones of aldehydes and ketones

1. Formaldehyde 2,4-DNPH 1. Formaldehyde 2,4-DNPH 2. Acetaldehyde 2,4-DNPH 2. Acetaldehyde 2,4-DNPH 3. Acetone 2,4-DNPH 3. Acetone 2,4-DNPH 4. Acrolein 2,4-DNPH 4. Acrolein 2,4-DNPH 5. Propionaldehyde 2,4-DNPH 5. Propionaldehyde 2,4-DNPH 6. Crotonaldehyde 2,4-DNPH 6. Crotonaldehyde 2,4-DNPH 7. Methylethylketone 2,4-DNPH 7. Methylethylketone 2,4-DNPH 8. Isobutyraldehyde 2,4-DNPH 8. Isobutyraldehyde 2,4-DNPH 9. Benzaldehyde 2,4-DNPH 9. Benzaldehyde 2,4-DNPH 10. n-Valeraldehyde 2,4-DNPH 10. n-Valeraldehyde 2,4-DNPH 11. p-Tolualdehyde 2,4-DNPH 11. p-Tolualdehyde 2,4-DNPH 12. Capronaldehyde 2,4-DNPH 12. Capronaldehyde 2,4-DNPH

(T920302A) (T931130D) ™ Column : YMC-Pack C4 (5µm, 120Å) 150 x 4.6 mm I.D. Column : J’Sphere™ ODS-H80 (4µm, 80Å) Part number : BU12SO51546WT 75 x 4.6 mm I.D. Eluent : A)tetrahydrofuran/water (10/90) Part number : JH08S04L546WT B)acetonitrile Eluent : A)tetrahydrofuran/water (20/80) 35%B (0-7min), 35-65%B (7-18min, linear), 100%B (18-19min), B)acetonitrile/methanol (90/10) 35%B (19-35min) 30-67%B (0-15min, Linear), 30%B (15-25min) Flow rate : 1.5 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 40 ˚C Detection : UV at 360 nm, 0.01 AUFS Detection : UV at 360 nm, 0.01 AUFS Injection : 11 µL (0.0025 mg/mL) Injection : 15 µL (0.001 mg/mL)

Polyaromatic hydrocarbons Polyaromatic hydrocarbons

1. Benzene 1. Benzene 2. Naphthalene 2. Naphthalene 3. 7,12-Dimethylbenzo[a]anthracene 3. Acenaphthylene 4. Acenaphthylene 4. 7,12-Dimethylbenzo[a]anthracene 5. Diphenyl 5. Diphenyl 6. Fluorene 6. Fluorene 7. Naphthacene 8. Acenaphthene 7. Acenaphthene 9. Phenanthrene 8. Naphthacene 10. Anthrathene 9. Phenanthrene 11. Fluoranthene 10. Anthrathene 12. Pyrene 11. Fluoranthene 13. Triphenylene 12. Pyrene 14. Chrysene 13. Triphenylene 15. Perylene 14. Chrysene 16. Benzo[e]pyrene 17. Benzo[b]fluoranthene 15. Perylene 18. Benzo[k]fluoranthene 16. Benzo[e]pyrene 19. Benzo[a]pyrene 17. Benzo[b]fluoranthene 20. Dibenzo[a,h]anthracene 18. Benzo[k]fluoranthene 21. Benzo[g,h,i]perylene 19. Benzo[a]pyrene 22. Coronene 20. Dibenzo[a,h]anthracene 21. Benzo[g,h,i]perylene 22. Coronene

(K930918A) (K930918B)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : JH08S042546WT Part number : JM08S042546WT Eluent : acetonitrile/water (65/35) Eluent : acetonitrile/water (65/35) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.08 AUFS Detection : UV at 254 nm, 0.08 AUFS Injection : 7 µL Injection : 7 µL

Polyaromatic hydrocarbons

1. Benzene 2. Naphthalene 3. 7,12-Dimethylbenzo[a]anthracene 4. Acenaphthylene 5. Diphenyl 6. Fluorene 7. Naphthacene 8. Acenaphthene 9. Phenanthrene 10. Anthrathene 11. Fluoranthene 12. Pyrene 13. Triphenylene 14. Chrysene 15. Perylene 16. Benzo[e]pyrene 17. Benzo[b]fluoranthene 18. Benzo[k]fluoranthene 19. Benzo[a]pyrene 20. Dibenzo[a,h]anthracene 21. Benzo[g,h,i]perylene 22. Coronene

(K930918C)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. Part number : JH08S041546WT Eluent : A)acetonitrile/water (50/50) B)acetonitrile 30%B (0-5min), 30-100%B (5-40min), 100%B (40-50min) Flow rate : 1.0 mL/min Temperature : 37 ˚C Detection : UV at 254 nm, 0.08 AUFS Injection : 7 µL

Benzoic acid derivatives Methoxybenzoic acid isomers

1. p-Ethoxybenzoic acid

1. o-Methoxybenzoic acid (o-Anisic acid)

2. p-Propoxybenzoic acid

2. p-Methoxybenzoic acid (p-Anisic acid) 3. p-Isopropylbenzoic acid (Cuminic acid)

3. m-Methoxybenzoic acid 4. p-Buthoxybenzoic acid (m-Anisic acid)

5. p-tert -Butylbenzoic acid

(O950511C) (O950508A)

Column : J’Sphere™ ODS-L80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. 75 x 4.6 mm I.D. Part number : JL08S041546WT Part number : JH08S04L546WT Eluent : tetrahydrofuran/water/acetic acid (40/60/1) Eluent : acetonitrile/water/acetic acid (20/80/1) Flow rate : 0.5 mL/min Flow rate : 1.0 mL/min Temperature : ambient (26 ˚C) Temperature : ambient (25 ˚C) Detection : UV at 260 nm, 0.32 AUFS Detection : UV at 285 nm, 0.16 AUFS Injection : 10 µL (0.04 ~ 0.2 mg/mL) Injection : 10 µL (0.17 mg/mL

Methoxybenzoic acid isomers Chlorobenzoic acid isomers

1. o-Methoxybenzoic acid (o-Anisic acid) 1. o-Chlorobenzoic acid

2. m-Chlorobenzoic acid 2. p-Methoxybenzoic acid (p-Anisic acid)

3. p-Chlorobenzoic acid 3. m-Methoxybenzoic acid (m-Anisic acid)

(O950508B) (O950509B)

Column : J’Sphere™ ODS-M80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 75 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JM08S05L546WT Part number : JH08S041546WT Eluent : acetonitrile/water/acetic acid (20/80/1) Eluent : acetonitrile/water/acetic acid (25/75/1) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (25 ˚C) Temperature : ambient (27 ˚C) Detection : UV at 285 nm, 0.16 AUFS Detection : UV at 270 nm, 0.04 AUFS Injection : 10 µL (0.17 mg/mL) Injection : 10 µL (0.17 mg/mL)

Hydroxybenzoic acids Resorcylic acid isomers

1. 3,5-DHBA 4. 2,5-DHBA 7. 2,3-DHBA 1. α-Resorcylic acid

8. Benzoic acid 2. 3,4-DHBA 5. 2,4-DHBA 2. β-Resorcylic acid

3. p-HBA 6. m-HBA 9. Salicylic acid 3. Resorcinol

DHBA, Dihydroxybenzoic acid HBA, Hydroxybenzoic acid

4. γ-Resorcylic acid

(O940530D) (O940607C)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : YMC-Pack™ C8 (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S041546WT Part number : OC12S051546WT Eluent : acetonitrile/water/acetic acid (5/90/5) Eluent : methanol/100mM KH PO (10/90) Flow rate : 1.0 mL/min 2 4 Flow rate : 0.8 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 285 nm, 0.16 AUFS Detection : UV at 285 nm, 0.16 AUFS Injection : 5 µL (0.1 mg/mL) Injection : 10 µL (0.05 ~ 0.4 mg/mL)

Resorcylic acid isomers Benzoic acid derivatives

1. p-Ethoxybenzoic acid

1. α-Resorcylic acid

2. p-Propoxybenzoic acid

2. β-Resorcylic acid

3. p-Isopropylbenzoic acid (Cuminic acid) 3. Resorcinol

4. p-Buthoxybenzoic acid 4. γ-Resorcylic acid

5. p-tert-Butylbenzoic acid

(O940606J) (O950511B)

Column : YMC-Pack™ Phenyl (5µm, 120Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : PH12S051546WT Part number : JM08S041546WT Eluent : tetrahydrofuran/water/acetic acid (40/60/1) Eluent : methanol/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Flow rate : 0.5 mL/min Temperature : 37 ˚C Temperature : ambient (26 ˚C) Detection : UV at 285 nm, 0.16 AUFS Detection : UV at 260 nm, 0.32 AUFS Injection : 10 µL (0.05 ~ 0.4 mg/mL) Injection : 10 µL (0.04 ~ 0.2 mg/mL)

Phenol derivatives Phenol derivatives

1. o-Nitrophenol 1. 2,6-Dinitrophenol

2. o-Chlorophenol

2. 2,4,6-Trinitrophenol

3. m-Nitrophenol

3. 2,4-Dinitrophenol

4. 2,6-Dichlorophenol 4. p-Nitrophenol

5. m-Chlorophenol 5. 2,4-Dichlorophenol

6. p-Chlorophenol 6. 2,4,6-Trichlorophenol

(O950616I) (O950614C)

Column : J’Sphere™ ODS-H80 (4µm, 80Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 75 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : JH08S04L546WT Part number : JH08S041546WT Eluent : 2-propanol/water/TFA (40/60/0.1) Eluent : tetrahydrofuran/water/acetic acid (35/65/1) Flow rate : 0.4 mL/min Flow rate : 0.5 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 230 nm, 0.13 AUFS Detection : UV at 240 nm, 0.13 AUFS Injection : 5 µL (0.04 mg/mL) Injection : 4 µL (0.08 mg/mL)

Phenol derivatives Cresol isomers

1. o-Nitrophenol

1. m-Cresol 2. o-Chlorophenol

3. m-Nitrophenol 2. p-Cresol

4. p-Nitrophenol 3. o-Cresol

5. m-Chlorophenol

(O950614F) (O951025B) 6. p-Chlorophenol Column : YMC-Pack ODS-AM™ (5µm, 120Å) Column : J’Sphere™ ODS-H80 (4µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AM12S051546WT Part number : JH08S041546WT Eluent : tetrahydrofuran/water/acetic acid (35/65/1) Eluent : acetonitrile/water (18/82) Flow rate : 0.5 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 240 nm, 0.13 AUFS Detection : UV at 260 nm, 0.064 AUFS Injection : 4 µL (0.08 mg/mL) Injection : 10 µL (0.3 mg/mL)

Chlorobenzoic acid isomers Polyphenols

2 1. Pyrogallol 2. Hydroquinone 3. Catechol 4. Phenol 1. o-Chlorobenzoic acid 1 3

4 2. m-Chlorobenzoic acid

3. p-Chlorobenzoic acid

0 10 20min

(H890627A) (O950509D) Column : YMC-Pack™ Phenyl (5µm, 120Å) ™ Column : YMC-Pack ODS-AM (5µm, 120Å) 250 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : PH12S052546WT Part number : AM12S051546WT Eluent : 5mM acetic acid Eluent : acetonitrile/water/acetic acid (25/75/1) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 25 ˚C Temperature : ambient (27 ˚C) Detection : UV at 280 nm, 0.32 AUFS Detection : UV at 270 nm, 0.04 AUFS Sample : reagents Injection : 10 µL (0.17 mg/mL) Injection : 20 µL

Dimethylphenol isomers Dimethylphenol isomers

1. 2,6-Dimethylphenol 4. 2,5-Dimethylphenol 1. 2,6-Dimethylphenol 4. 2,5-Dimethylphenol

2. 2,4-Dimethylphenol 5. 2,3-Dimethylphenol 5. 2,3-Dimethylphenol 2. 2,4-Dimethylphenol

3. 3,5-Dimethylphenol 6. 3,4-Dimethylphenol 6. 3,4-Dimethylphenol 3. 3,5-Dimethylphenol

(T921019B) (T921020C)

™ ™ Column : YMC-Pack Polyamine II Column : YMC-Pack NH2 (5µm, 120Å) 250 x 4.6 mm I.D. 250 x 4.6 mm I.D. Part number : PB12S052546WT Part number : NH12S052546WT Eluent : 20% ethyl acetate in hexane Eluent : 10% ethyl acetate in hexane Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 30 ˚C Temperature : 30 ˚C Detection : UV at 254 nm, 0.08 AUFS Detection : UV at 254 nm, 0.08 AUFS Injection : 20 µL (0.33 mg/mL) Injection : 20 µL (0.33 mg/mL

Aminoalcohols Urea and related compounds

1. Ethanolamine 1. Urea 2. Diethanolamine 2. Propionamide 3. N-Methylethanolamine 3. Ethylurea 4. Triethanolamine 4. 1,3-Diethylurea 5. N,N-Dimethylethanolamine 5. 1,1-Diethylurea

(M920124B) (M920108A)

Column : YMC-Pack PolymerC18™ Column : YMC-Pack PolymerC18™ 250 x 6.0 mm I.D. 250 x 6.0 mm I.D. Part number : PC99S062506WT Part number : PC99S062506WT

Eluent : 100mM Na2HPO4/100mM NaOH (60/40, pH 12.0) Eluent : 0.4% HClO4 (pH 1.3) Flow rate : 0.6 mL/min Flow rate : 0.5 mL/min Temperature : 20 ˚C Temperature : 25 ˚C Detection : UV at 215 nm, 0.32 AUFS Detection : UV at 210 nm, 0.32 AUFS Injection : 50 µL (0.2 ~ 3.0 mg/mL) Injection : 15 µL (0.4 ~ 7.5 mg/mL)

A nonionic detergent Polyethyleneglycols

1. Triton X-100 1. PEG #20,000 (MW 20,000) 2. PEG #6,000 (MW 8,400) 3. PEG #4,000 (MW 3,350) 4. PEG #1,000 (MW 1,000) 5. PEG #300 (MW 300) 6. Ethyleneglycol (MW 62)

(G920109C) (G920123A) Column : YMC-Pack™ Diol-120 ™ 500 x 8.0 mm I.D. Column : YMC-Pack Diol-120 Part number : DL12S055008WT 500 x 8.0 mm I.D. Eluent : acetonitrile/water (10/90) Part number : DL12S055008WT Flow rate : 1.0 mL/min Eluent : acetonitrile/water(10/90) Temperature : ambient (23 ˚C) Flow rate : 1.0 mL/min Detection : UV at 260 nm, 0.02 AUFS Temperature : ambient (22 ˚C) Injection : 15 µL (5.0 mg/mL) Detection : RI, 64 x 10-6 RIU/FS Sample : Triton X-100 Injection : 100 µL (1.67 mg/mL)

Cresol isomers Metal-PAN chelates

1. m-Cresol

1. Co (II)-1- (2-Pyridylazo)-2-naphthol 2. Ni (II)-1- (2-Pyridylazo)-2-naphthol 2. p-Cresol 3. Fe (III)-1- (2-Pyridylazo)-2-naphthol 4. Zn (II)-1- (2-Pyridylazo)-2-naphthol

3. o-Cresol

(T910520A) (O951025C) Column : YMC-Pack ODS-AM™ (5µm, 120Å) Column : J’Sphere™ ODS-M80 (4µm, 80Å) 150 x 6.0 mm I.D. 150 x 4.6 mm I.D. Part number : AM12S051506WT Part number : JM08S041546WT Eluent : acetonitrile/water (70/30) containig 0.01% PAN Eluent : acetonitrile/water (18/82) and 10mM tetrabutylammonium bromide Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Detection : UV at 260 nm, 0.064 AUFS Detection : VIS at 565 nm, 0.08 AUFS Injection : 10 µL (0.3 mg/mL) Injection : 12 µL (25 ~ 92 µg/mL)

Coordination compounds Hinokitiol

1. Uracil 2. Acetylacetone 3. 8-Quinolinol

1. Hinokitiol

2. 1-Naphthol(I.S.)

(M910604I) (O960507F)

Column : YMC-Pack ODS-AM™ (5µm, 120Å) Column : YMC-Pack ODS-AM™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AM12S051546WT Part number : AM12S051546WT

Eluent : 20mM KH2PO4 - K2HPO4 (pH7.6)/methanol (40/60) Eluent : 20mM NH4H2PO4 containing 1mM EDTA•2Na/acetonitrile (65/35) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 40 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.32 AUFS Detection : UV at 320 nm, 0.16 AUFS Injection : 10 µL (0.08, 0.25 mg/mL

Phthalic acid isomers Phthalic acid isomers

1. Phthalic acid

2. Terephthalic acid 2. Terephthalic acid

3. Isophthalic acid 3. Isophthalic acid

(A971112B) (A971112A)

Column : YMC-Pack Pro C18™ (5µm, 120Å) Column : YMC-Pack ODS-AQ™ (5µm, 120Å) 150 x 3.0 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051503WT Part number : AQ12S051546WT Eluent : methanol/10mM H PO (25/75) 3 4 Eluent : methanol/10mM H3PO4 (25/75) Flow rate : 0.4 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.16 AUFS Detection : UV at 254 nm, 0.16 AUFS Injection : 4 µL (0.05 ~ 0.5 mg/mL) Injection : 10 µL (0.05 ~ 0.5 mg/mL)

Chlorophenol isomers Chloracetophenone isomers 1. OH 1. COCH Cl 3 Cl 2 2 o-Chlorophenol o-Chloroacetophenone

2. 2. OH COCH3 3

3 Cl 1 Cl 1 p-Chlorophenol p-Chloroacetophenone

3. 3. OH COCH3

Cl Cl m-Chlorophenol m-Chloroacetophenone

0 10 20 min 0 10 20 min

(J011113D) (J011026B)

Column : YMC-Pack Pro C18 RS™ (5µm, 80Å) Column : YMC-Pack Pro C18 RS™ (5µm, 80Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : RS08S051546WT Part number : RS08S051546WT Eluent : acetonitrile/water/acetic acid (30/70/1) Eluent : acetonitrile/ water (40/60) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 30 ˚C Pressure : 8.8MPa Pressure : 9.7 MPa Detection : UV at 230 nm, 0.31 AUFS Detection : UV at 254 nm, 0.32 AUFS Injection : 10 µL (0.1 ~ 0.2 mg/mL) Injection : 8.0 µL (0.05 ~ 0.1 µl/mL)

Fullerenes Fullerenes

1. Buckminsterfullerene(C60)

2. (5,6)-Fullerene(C70)

(S930119B) (S930128B)

™ Column : YMC-Pack ODS-A™ (5µm, 120Å) Column : YMC-Pack Phenyl (5µm, 120Å) 150 x 6.0 mm I.D. 150 x 6.0 mm I.D. Part number : AA12S051506WT Part number : PH12S051506WT Eluent : hexane/2-propanol (70/30) Eluent : hexane/2-propanol (50/50) Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : ambient (25 ˚C) Temperature : ambient (25 ˚C) Detection : UV at 350 nm, 0.08 AUFS Detection : UV at 350 nm, 0.08 AUFS Injection : 4 µL (0.125 mg/mL) Injection : 4 µL (0.125 mg/mL)

Aromatic amines Aromatic amines

1. p-Phenylenediamine 1. p-Phenylenediamine

2. p-Aminophenol 2. p-Aminophenol

3. m-Phenylenediamine 3. m-Phenylenediamine

4. m-Aminophenol 4. m-Aminophenol

5. o-Phenylenediamine 5. o-Phenylenediamine

6. o-Aminophenol 6. o-Aminophenol (S961007C) (S961004E)

Column : YMC-Pack Pro C18™ (5µm, 120Å) Column : YMC-Pack ODS-AM™ (5µm, 120Å) 150 x 4.6 mm I.D. 150 x 4.6 mm I.D. Part number : AS12S051546WT Part number : AM12S051546WT Eluent : 50mM NH H PO - (NH ) HPO (pH 6.7)/methanol (95/5) Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/methanol (95/5) 4 2 4 4 2 4 Flow rate : 1.0 mL/min Flow rate : 1.0 mL/min Temperature : 37 ˚C Temperature : 37 ˚C Detection : UV at 254 nm, 0.26 AUFS Detection : UV at 254 nm, 0.26 AUFS Injection : 5 µL (0.125 ~ 0.5 mg/mL) Injection : 5 µL (0.125 ~ 0.5 mg/mL)

Terphenyl isomers 1.

2 o-Terphenyl

1 m-Terphenyl 3

p-Terphenyl

0 10 20 min

(J020719E)

Column : YMC-Pack Pro C18™ RS (5µm, 80Å) 150 x 4.6 mm I.D. Part number : RS08S051546WT Eluent : methanol/water (85/15) Flow rate : 1.0 mL/min Temperature : 37 ˚C Pressure : 9.2 MPa Detection : UV at 254 nm, 0.16 AUFS Injection : 10 µL (0.02mg/mL)

Index of Analytes

Apronalide ...... 88 A Arabitol ...... 33 Arachidonic acid ...... 61 Arbutin ...... 69,75 Acacetin ...... 72 Arginine ...... 48 Acenaphthene ...... 92,93,117,118 L-Arginine ...... 48, 49 Acenaphthylene ...... 117,118 L-Ascorbic acid ...... 25, 26, 28, 31 Acetaldehyde 2,4-DNPH ...... 117 L-Ascorbic acid 2,4-DNPH ...... 31 Acetaminophen ...... 87,88 Asparagine ...... 48 Acetic acid ...... 60 L-Asparagine ...... 49 Acetoacetyl-CoA ...... 31 Aspartame ...... 66, 67 Acetone 2,4-DNPH ...... 117 Aspartic acid ...... 48 Acetylacetone ...... 124 L-Aspartic acid ...... 48, 49 Acetyl-CoA ...... 31 Asulam ...... 113 N -Acetylprocainamide ...... 98 5'-ATP ...... 45 Acid red ...... 65 Atraton ...... 114 Acrolein 2,4-DNPH ...... 117 Atrazine ...... 114,115 Acyclovir ...... 99 Atrazine-desethyl ...... 114 Adenine ...... 30,43 Atrazine-desisopropyl ...... 114 Adenosine ...... 43 Atrazine-2-hydroxy ...... 114 Adenylate kinase ...... 56-58 Atropine sulfate ...... 68 5'-ADP ...... 45 AZT ...... 100 ADP-D-glucose ...... 46,47

Aflatoxin B1 ...... 76 Aflatoxin B ...... 76 2 B Aflatoxin G1 ...... 76 Aflatoxin G2 ...... 76 Alachlor ...... 115 Bacampicillin hydrochloride ...... 111 Alanine ...... 49 Baicalein ...... 72 L-Alanine ...... 48,49 Baicalin ...... 69, 72 Albiflorin ...... 69 BAM-12P ...... 51 Aldicarb ...... 116 Barbaloin ...... 69 Allantoic acid ...... 102 Beclomethasone ...... 78,80 Allantoin ...... 29,102 Beclomethasone dipropionate ...... 80 Allopurinol ...... 99 Bendiocarb ...... 116 Amaranth ...... 65 Benfotiamine ...... 29, 30 Ametryn ...... 114 Bensulide ...... 113 Amino Acids ...... 48,49 Benzaldehyde 2,4-DNPH ...... 117 p -Aminobenzoic acid (PABA) ...... 27, 29 Benzalkonium chloride ...... 89 o -Aminopehnol ...... 126 Benzene ...... 77,117,118 Benzethonium chloride ...... 89, 90 p -Aminophenol ...... 126 Benzo[a]pyrene ...... 117,118 Aminopterin ...... 96 Benzo[b]fluoranthene ...... 117,118 Amitriptyline hydrochloride ...... 92,93 Benzo[e]pyrene ...... 117,118 Amoxicillin ...... 111 Benzo[g,h,i]perylene ...... 117,118 5'-AMP ...... 45,46 Benzo[k]fluoranthene ...... 117,118 Ampicillin ...... 105, 111 Benzocaine ...... 97 Androsterone ...... 81 Benzoic acid ...... 62,66,67,98 trans -Androsterone ...... 81 Benzylpenicillin potassium ...... 111 [Asn1,Val5] Angiotensin I ...... 49 Bepridil HCl ...... 94 [Val5] Angiotensin I ...... 49 Betamethasone ...... 78, 80 Angiotensin I ...... 49,50,51 Betamethasone valerate ...... 80 Angiotensin II ...... 49,50,52,55,56 Bezafibrate ...... 95 Angiotensin III ...... 49,50,52,53 BHA (Butyl hydroxyanisol) ...... 65 o -Anisic acid ...... 119 BHT (Dibutyl hydroxytoluene) ...... 65 m -Anisic acid ...... 119 1,1,-Bi-2-naphthol ...... 124 p -Anisic acid ...... 119 Biotin ...... 27 Annatto extract ...... 66 Bisbentiamine ...... 30 Anthrathene ...... 117,118 Bombesin ...... 51 Antioxidants ...... 63-65 Bradykinin ...... 51-53, 58,59 Apigenin ...... 72 Brassicasterol ...... 84

Index of Analytes

Brilliant Blue FCF ...... 65 Chlorophyll b ...... 68 Bromovalerylurea ...... 87,90 Chloropropham ...... 115 BSA ...... 49 Chlorotetracycline ...... 105 Bucetine ...... 87, 88 Chloroheniramine ...... 89 Bufalin ...... 86 Chlorpheniramine maleate ...... 87,88,90,91 Butacaine ...... 97 Chlorpromazine hydrochloride ...... 92 Butachlor ...... 115 Chlorthalidone ...... 96,97 p -Buthoxybenzoic acid ...... 119,120 Cholecalciferol ...... 21,22 p-tert-Butylbenzoic acid ...... 119,120 Cholesterol ...... 84 tert -Butylhydroquinone (TBHQ) ...... 63, 64 Cholic acid (CA) ...... 83 Butyl hydroxyanisol (BHA) ...... 64 Chondroitin, Na salt ...... 41 Butyl p-hydroxybenzoate ...... 62, 63, 76, 91 Chondroitin sulfate A, Na salt ...... 41 Chondroitin sulfate B, Na salt ...... 41 Chondroitin sulfate C, Na salt ...... 42 C Chrysene ...... 117,118 Chrysin ...... 72 Chrysoeriol ...... 72 Caffeine ...... 26,66,67,68, 87, Cinobufagin ...... 86 ...... 89,90,88,111,112 Cinchonine ...... 68,97 Campesterol ...... 84 Cinoxacin ...... 105,106 Capronaldehyde 2,4-DNPH ...... 117 Citric acid ...... 60 Carbadox ...... 104 Clofibrate ...... 95 Carbamazepine ...... 92 Cloxacillin sodium ...... 111 Carnitine chloride ...... 99 5'-CMP ...... 45, 46 α−Carotene ...... 21 Codeine ...... 90 β−Carotene ...... 21,22 Coenzyme A ...... 31 Casein ...... 56 Coenzyme A glutathione disulfide ...... 31 (+)-Catechin ...... 73,74 Coenzyme A oxidized ...... 31 Catechol ...... 122 Color additives (Acid red) ...... 65 Catecholamines ...... 102 Color additives (Amaranth) ...... 65 CCK-Octapeptide ...... 52 Color additives (Brilliant Blue FCF) ...... 65 5'-CDP ...... 45 Color additives (Erythrosine) ...... 65 CDP-D-glucose ...... 46,47 Color additives (Fast Green FCF) ...... 65 Cefaclor ...... 106, 108 Color additives (Indigo Carmine) ...... 65 Cefadroxil ...... 107,108 Color additives (New Coccine) ...... 65 Cefamandole sodium ...... 107,110 Color additives (Phloxine) ...... 65 Cefatrizine propylene glycol ...... 107,108 Color additives (Rose Bengale) ...... 65 Cefazolin sodium ...... 106, 108 Color additives (Sunset Yellow FCF) ...... 65 Cefoperazone sodium ...... 107,110 Color additives (Tartrazine) ...... 65 Cefotaxime sodium ...... 106, 108-110 Copper 8-quinolinolate ...... 114 Cefoxitin sodium ...... 106,108,111,112 Coronene ...... 117,118 Cefsulodin sodium ...... 106,108 Corticosterone ...... 77-80 Ceftriaxone sodium ...... 109,110,112 Corticosterone acetate ...... 77,80 Cefuroxime sodium ...... 106,108 Cortisone ...... 77-80 Cephalexin hydrate ...... 105-108 Cortisone acetate ...... 77,80 Cephaloglycin ...... 106,108,109 o -Cresol ...... 121,124 Cephaloridine ...... 106-108 m -Cresol ...... 121,124 Cephalosporin C potassium ...... 107,108,112 p -Cresol ...... 121,124 Cephalothin sodium ...... 107,110 Crimidine ...... 114 Cephapirin sodium ...... 106,108,109 Crotonaldehyde 2,4-DNPH ...... 117 Cephradine ...... 106,108 5'-CTP ...... 45 Chenodeoxycholic acid (CDAC) ...... 83 Cuminic acid ...... 119,120 Chloramphenichol ...... 105 Cyanazine ...... 114 Chlorhexidine ...... 101 Cyanocobalamine ...... 25,27,29 o -Chlorobenzoic acid ...... 119,122 Cyclodextrins ...... 37,38 m -Chlorobenzoic acid ...... 119,122 α-Cyclodextrin ...... 37,38 p -Chlorobenzoic acid ...... 87,119,122 β-Cyclodextrin ...... 37,38 o -Chlorophenol ...... 121 γ-Cyclodextrin ...... 37,38 m -Chlorophenol ...... 121 L-Cystine ...... 48,49 p -Chlorophenol ...... 121 Cytidine ...... 43 Chlorophyll a ...... 68 Cytochrome c ...... 55-58

Cytochrome c (dimer) ...... 55 O -Dimethyl-β-cyclodextrin ...... 38 Cytochrome c (hexamer) ...... 55 N,N -Dimethylethanolamine ...... 123 Cytochrome c (monomer) ...... 55 2,3-Dimethylphenol ...... 122 Cytochrome c (tetramer) ...... 55 2,4-Dimethylphenol ...... 122 Cytochrome c (trimer) ...... 55 2,5-Dimethylphenol ...... 122 Cytocine ...... 43 2,6-Dimethylphenol ...... 122 3,4-Dimethylphenol ...... 122 3,5-Dimethylphenol ...... 122 D 2,4-Dinitrophenol ...... 121 2,6-Dinitrophenol ...... 121 Diphenhydramine hydrochloride ...... 91 D4T ...... 100 Diphenyl ...... 83,116-118 Daidzein ...... 73 Disaccharides ...... 32`` Daidzin ...... 73 Diuron ...... 114,115 5'-dAMP ...... 45 Docosahexaenoic acid ...... 61 5'-dCMP ...... 45 n -Dodecyl gallate (DG) ...... 63,64 DDC ...... 100 DOPA (3,4-Dihydroxyphenylalanine) ...... 102 DDI ...... 100 Dopamine hydrochloride ...... 102 Dehydroacetic acid ...... 62,67 Doxepin hydrochloride ...... 93 Dehydroisoandrosterone ...... 81 Doxorubicin hydrochloride ...... 105 Deoxyadenosine ...... 43 Dynorphin A (Human,1-13) ...... 51 Deoxycorticosterone ...... 77-80 Deoxycorticosterone acetate ...... 80 Deoxycytidine ...... 43 E Deoxyguanosine ...... 43 Deoxyinosine ...... 43 Deoxyuridine ...... 96 α-Ecdysone ...... 84 Desipramine hydrochloride ...... 92,93 β-Ecdysone ...... 84 Dexamethasone ...... 78,80 Cu (II)-EDTA ...... 63 5'-dGMP ...... 45 Fe (III)-EDTA ...... 63 ∆ Di-0S ...... 33 Eicosapentaenoic acid ...... 61 ∆ Di-4S ...... 33 Eicosatrienoic acid ...... 61 ∆ Di-6S ...... 33 Eledoisin related peptide ...... 51 -Endorphin ...... 51 ∆ Di-diSD ...... 33 α -Endorphin (Human) ...... 51 ∆ Di-diSE ...... 33 β ∆ Di-triS ...... 33 γ-Endorphin ...... 51 Diaveridine ...... 104 [D-Ala2,Met5]-Enkephalin ...... 51 Dibenzo[a,h]anthracene ...... 117,118 Leu-Enkephalin ...... 49,51-53,58,59 Dibutyl hydroxytoluene (BHT) ...... 64 Met-Enkephalin ...... 49,51-53 Dicethiamine HCl ...... 30 [D-Ala2,Met5]-Enkephalinamide ...... 51 2,4-Dichlorophenol ...... 121 Enolase ...... 56-58 2,6-Dichlorophenol ...... 121 Enoxacin ...... 105 Dicloxacillin sodium ...... 111 (-)-Epicatechin ...... 73,74 Diethanolamine ...... 123 (-)-Epicatechin gallate ...... 73,74 1,1-Diethylurea ...... 123 (-)-Epigallocatechin ...... 73,74 1,3-Diethylurea ...... 123 (-)-Epigallocatechin gallate ...... 73,74 Digitoxigenin ...... 85 Epinephrine ...... 102 Digitoxin ...... 69,85 Ergocalciferol ...... 21,22 Digoxigenin ...... 85 Erythorbic acid ...... 28,31 Digoxigenin-bis-digitoxoside ...... 85 Erythritol ...... 33 Digoxin ...... 85 Erythromycin ...... 113 2,3-Dihydroxybenzoic acid ...... 120 Erythromycin estolate ...... 113 2,4-Dihydroxybenzoic acid ...... 120 Erythromycin ethylsuccinate ...... 113 2,5-Dihydroxybenzoic acid ...... 120 Erythrosine ...... 65 3,4-Dihydroxybenzoic acid ...... 120 Esculine ...... 69 3,5-Dihydroxybenzoic acid ...... 120 β-Estradiol ...... 82,83 3,4-Dihydroxyphenylacetic acid ...... 102 Estradiol benzoate ...... 82,83 3,4-Dihydroxyphenylalanine (DOPA) ...... 102 Estradiol dipropionate ...... 82,83 Diltiazem HCl ...... 94, 97,101 Estriol ...... 82 7,12-Dimethylbenzo[a]anthracene ...... 117,118 Estrone ...... 82

Ethanolamine ...... 123 Ginsenoside-Rg1 ...... 71 Ethenzamide ...... 87,88,90 Glucose (G1) ...... 32,35,37,46,47 Ethinylestradiol ...... 82,83 Glutamate dehydrogenase ...... 56-58 Ethiofencarb ...... 116 Glutamic acid ...... 48 Ethopropazine hydrochloride ...... 92 L-Glutamic acid ...... 48,49 Ethosuximide ...... 92 Glutamine ...... 48 p -Ethoxybenzoic acid ...... 119,120 Glutathione (GSH) ...... 49 Ethyl 4-aminobenzoate ...... 97 Glutathione disulfide (GSSG) ...... 49 Ethylbenzene ...... 80 Glycerol ...... 33 Ethyleneglycol ...... 123 Glycine ...... 48,49,55,56 Ethyl p-hydroxybenzoate ...... 62,63 Glycolic acid ...... 60 Ethylurea ...... 123 Glycosides ...... 69 Glycyrrhizic acid (Glycyrrhizin) ...... 67,69,89 5'-GMP ...... 45,46 F 5'-GTP ...... 45 Guanine ...... 43 Guanosine ...... 43 Fast Green FCF ...... 65 Fenofibrate ...... 95 Fenoprofen calcium ...... 93 H Flavin adenine dinucleotide disodium ...... 30 Flavin mononucleotide sodium salt (Flavin sodium phosphate) ...... 26,30 Heparan sulfate, Na salt ...... 42 Flavinoids ...... 71,72,73 Heparin, Na salt ...... 42 Fluocinolone acetonide ...... 77-80 Hesperidin ...... 72,73 Fluocinonide ...... 78-80 Hexazinone, ...... 114 Fluoranthene ...... 117,118 Hinokitiol ...... 124 Fluorene ...... 117,118 Hippuric acid ...... 101,102 5-Fluorocytosine ...... 43 Histidine ...... 48 5-Fluoro-2'-deoxyuridine ...... 96 L-Histidine,Hydrachloride ...... 48,49,99 5-Fluoroorotic acid ...... 44 Homovanilic acid ...... 102 5-Fluorouracil ...... 44, 96 Hordenine sulfate ...... 68 Fluoxymesterone ...... 80,81 Human IgA ...... 53,54 Fluphenazine hydrochloride ...... 92 Human IgG ...... 53,54 Flurbiprofen ...... 93,101 Human IgM(κ),myeloma protein ...... 53,54 Folic acid ...... 25,96 Hyaluronic acid, Na salt ...... 42 Formaldehyde 2,4-DNPH ...... 117 Hydrochlorothiazide ...... 96,97 1-Fructofuranosyl-D-nystose ...... 34 Hydrocortisone ...... 77-80 Fructooligusaccharides ...... 34 Hydrocortisone acetate ...... 77,80 Fructose ...... 32 Hydrocortisone succinate ...... 79 Fucose ...... 32 Hydroquinone ...... 75,122 Fucosterol ...... 84 4'-Hydroxyacetophenone ...... 69,98,113

Fullerene (C60) ...... 126 m -Hydroxybenzoic acid ...... 120 Fullerene (C70) ...... 126 p -Hydroxybenzoic acid ...... 87,120 Fumaric acid ...... 60 4-Hydroxy-3-methoxymandelic acid ...... 102 Furazolidone ...... 104 4-Hydroxy-3-methoxyphenylglycol ...... 102 Furosemide ...... 96,97 4-Hydroxymethyl-2,6-di-tert-butylphenol (HMBP) ...... 63,64 3-Hydroxy-3-methylglutaryl-CoA G (HMG-CoA) ...... 31 Hyodeoxycholic acid (HDCA) ...... 83 Hypoxanthine ...... 43,99 Galactose ...... 32 Gallic acid ...... 75 5'-GDP ...... 45 I GDP-D-mannose ...... 46,47 Gemfibrozil ...... 95 Genistein ...... 73 Ibuprofen ...... 90,93 Genistin ...... 73 Imazalil ...... 116

Ginsenoside-Rb1 ...... 71 Imipramine hydrochloride ...... 92,93

Immunoglobins ...... 53,54 5'-IMP ...... 45 M Indigo Carmine ...... 65 Indomethacin ...... 86,87 Inosine ...... 43 Maleic acid ...... 60,89 Inositol ...... 33 L-Malic acid ...... 60 Insulin ...... 49-52,55,56 Malonic acid ...... 60 Insulin (Bovine) ...... 50,51,55,56 Malonyl-CoA ...... 31 Insulin (Equine) ...... 50 Maltitol ...... 33

Insulin (Human) ...... 50,51 Maltododecaose (G12) ...... 35,36 Insulin (Porcine) ...... 50,51 Maltoheptaose (G7) ...... 35,37 Insulin (Sheep) ...... 50,51 Maltohexaose (G6) ...... 35 Insulin Chain B ...... 52 Maltooligosaccharides ...... 35-37

Isobutyraldehyde 2,4-DNPH ...... 117 Maltopentadecaose (G15) ...... 35-37 Isobutyl p-hydroxybenzoate ...... 62 Maltopentaose (G5) ...... 35,37 Isoleucine ...... 48 Maltose (G2) ...... 32,35 L-Isoleucine ...... 48,49 Maltotetradecaose (G14) ...... 35,36 Isonicotinic acid ...... 98,100 Maltotetraose (G4) ...... 35 Isonicotinic acid hydrazide ...... 98,100 Maltotridecaose (G13) ...... 35-37 Isophthalic acid ...... 125 Maltotriose (G3) ...... 37 p -Isopropylbenzoic acid ...... 119,120 Maltoundecaose (G11) ...... 35-37 Isopropyl p-hydroxybenzoate ...... 62 Mandelic acid ...... 101,102 Isoproturon ...... 114,115 Mannitol ...... 33 Mannose ...... 32,46,47 α-Mating factor ...... 49,51 K Medroxyprogesterone acetate ...... 82 Menadione ...... 21 Menaquinone ...... 21,25 Kaempferol ...... 71 Mestranol ...... 82 Keratan polysulfate, Na salt ...... 43 Metazachlor ...... 114,115 Keratan sulfate, Na salt ...... 43 Methicillin sodium ...... 111 1-Kestose ...... 33,34 Methionine ...... 48 Ketoprofen ...... 91,93 L-Methionine ...... 48,49 Methotrexate ...... 96 o -Methoxybenzoic acid ...... 119 L m -Methoxybenzoic acid ...... 119 p -Methoxybenzoic acid ...... 119 3-Methoxytyramine hydrochloride ...... 102 Lactate dehydrogenase ...... 56-58 Methoxyverapamil HCl ...... 94 Lactic acid ...... 60 Methyl Benzoate ...... 101 Lactose ...... 32 N -Methylethanolamine ...... 123 Lactosyl fructoside ...... 34,35 Methylethylketone 2,4-DNPH ...... 117 Lanatoside B ...... 85 o -Methylhippuric acid ...... 101,102 Lanatoside C ...... 85 m -Methylhippuric acid ...... 101,102 Leucine ...... 48 p -Methylhippuric acid ...... 101,102 L-Leucine ...... 48,49 Methyl p-hydroxybenzoate ...... 62,63,72 Leu-Enkephalin ...... 49,51-53 Methyl linoleate ...... 61 Lidocaine ...... 94,97,101 Methyl linolenate ...... 61 Linoleic acid ...... 61 Methyl γ-linolenate ...... 61 γ-Linolenic acid ...... 61 Methylmalonyl-CoA ...... 31 Linuron ...... 114,115 N' -Methylnicotinamide ...... 98,100 Lovastatin ...... 95 Methyl oleate ...... 61 Luteolin ...... 72 Methylprednisolone ...... 77,78,80 Lysine ...... 48 Methyltestosterone ...... 81 L-Lysine ...... 48,49 Metobromuron ...... 114,115 Lysozyme ...... 58 Metolachlor ...... 114,115 Metoxuron ...... 114,115 Monosaccharides ...... 32 Myoglobin ...... 55,58 Myricetin ...... 71

Oxamyl ...... 116 Oxipurinol ...... 99 N Oxolinic acid ...... 104,105,106 Oxytetracycline hydrochloride ...... 105,113 NAD ...... 30 Oxytocin ...... 51,58,59 NADH ...... 30 NADP ...... 30 NADPH ...... 30 P Nalidixic acid ...... 104-106 Naphazoline hydrochloride ...... 90 Naphthacene ...... 117,118 PABA ...... 27,29 Naphthalene ...... 117,118 Paeoniflorin ...... 69,70 1-Naphthol ...... 124 Paeonol ...... 76 Naproxen ...... 91 Pantothenic acid (Na salt) ...... 25 Narcotine ...... 68,87,88 Pantothenyl alcohol (Panthenol) ...... 26,30 Naringin ...... 72,73 Papaverine chloride ...... 68 Narirutin ...... 72,73 PA-Sugar Chain 001 ...... 38,39 Neohesperidin ...... 72,73 PA-Sugar Chain 002 ...... 38,39 Neostigmine methylsulfate ...... 89 PA-Sugar Chain 014 ...... 38,39 Neurotensin ...... 51,55,56 PEG #300 (MW 300) ...... 123 New Coccine ...... 65 PEG #1,000 (MW 1,000) ...... 123 Nicardipine HCl ...... 94,97 PEG #4,000 (MW 3,350) ...... 123 Nicotinamide ...... 25,26,28,29 PEG #6,000 (MW 8,400) ...... 123 ...... 30,98,100 PEG #20,000 (MW 20,000) ...... 123 β-Nicotinamide adenine dinucleotide ...... 30 Penicillin G potassium ...... 111 Nicotinamide adenine Penicillin V potassium ...... 111 dinucleotide phosphate oxidized form ...... 30 Pentaglycine ...... 56 Nicotinamide adenine dinucleotide Peptides ...... 49,51,52,53,55 phosphate reduced form ...... 30 Perphenazine ...... 92 β-Nicotinamide adenine Perylene ...... 117,118 dinucleotide reduced form ...... 30 Phenanthrene ...... 117,118 Nicotinic acid ...... 25,28,30, Phenethicillin potassium ...... 111 ...... 98,100 Phenobarbital sodium salt ...... 92 Nifedipine ...... 94 Phenol ...... 68,98,112,121,122 Niguldipine HCl ...... 94 Phenoxyethylpenicillin potassium ...... 111 o -Nitrophenol ...... 121 Phenoxymethylpenicillin potassium ...... 111 m -Nitrophenol ...... 121 Phenylalanine ...... 48 p -Nitrophenol ...... 121 L-Phenylalanine ...... 48,49 Nordihydroguaiaretic acid (NDGA) ...... 63,64 o -Phenylenediamine ...... 126 Norepinephrine hydrochloride ...... 102 m -Phenylenediamine ...... 126 Norethisterone ...... 82 p -Phenylenediamine ...... 126 Norfloxacin ...... 105 Phenylephrine HCl ...... 89 Norgestrel ...... 83 2-Phenylethyl β-cellobioside ...... 74 Nucleosides and bases ...... 43 2-Phenylethyl β-gentiobioside ...... 74 Nucleotides ...... 43-47 2-Phenylethyl β-lactoside ...... 74 Nystose ...... 33,34 2-Phenylethyl β-maltoside ...... 74 2-Phenylethyl β-melibioside ...... 74 2-Phenylethyl glycoside ...... 74 O o -Phenylphenol ...... 116 Phenylpropanolamine HCl ...... 89 Phenytoin ...... 92 n -Octyl gallate (OG) ...... 63 Phloxine ...... 65 Ofloxacin ...... 105 Phosphoenolpyruvic acid Olaquindox ...... 104 monopotassium salt ...... 60 Oligonucleotides (c-Ha-ras/12) ...... 46 Phthalic acid ...... 125 Oligosaccharides ...... 33,34,35 Phylloquinone ...... 21,25 Organic Acids ...... 60-61 Pilocarpine chloride ...... 68 Orotic acid ...... 28,44 Pipemidic acid ...... 105 Ouabain ...... 69,85 Pirimicarb ...... 116 Ouabagenin ...... 85 Piromidic acid ...... 104,105,106 Ovalubmin ...... 49 Piroxicam ...... 91

Plasmid pBR322 ...... 47 Rebaudioside A ...... 67 Poly (A) ...... 46 Resorcinol ...... 30,112,120 Polydextrose ...... 40 α -Resorcylic acid ...... 120 Prednisolone ...... 77-80 β -Resorcylic acid ...... 120 Prednisolone acetate ...... 78-80 γ -Resorcylic acid ...... 120 Prednisolone succinate ...... 79 Resibufogenin ...... 86 Prednisone ...... 77,78,80 Retinol ...... 21 Prenylamine lactate ...... 94 Retinol acetate ...... 21 Primidone ...... 92 Retinol palmitate ...... 21 Procainamide ...... 98 Rhamnose ...... 32 Procaine HCl ...... 97 Ribitol ...... 33 Prochlorperazine maleate ...... 92 Riboflavin ...... 25,27,29,30 Profenamine hydrochloride ...... 92 Ribonuclease A ...... 58 Progesterone ...... 77,82 Rose Bengale ...... 65 Proline ...... 48 L-Proline ...... 48,49 Promazine hydrochloride ...... 92 S Prometon ...... 114,115 Prometryn ...... 114,115 Propazine ...... 114,115 Saccharin sodium ...... 62 Propham ...... 114,115 Saikosaponin a ...... 70

Propicillin potassium ...... 111 Saikosaponin b2 ...... 70 Propionaldehyde 2,4-DNPH ...... 117 Saikosaponin c ...... 70 Propionamide ...... 123 Saikosaponin d ...... 70 Propionic acid ...... 60 Salicin ...... 69 Propionyl-CoA ...... 31 Salicylic acid ...... 98,120 p -Propoxybenzoic acid ...... 119,120 Saponins ...... 70 Propranolol hydrochloride ...... 94,97 Scopolamine bromide ...... 68 n -Propyl gallate (PG) ...... 63,64 Sebuthylazine ...... 114,115 Propyl p-hydroxybenzoate ...... 62,63,67 Sennoside A ...... 74,75 Proscillaridin A ...... 85 Sennoside B ...... 74,75 Pullulan P-5 (MW 10,700) ...... 40 Serine ...... 48 Pullulan P-20 (MW 23,700) ...... 40 L-Serine ...... 48,49 Pullulan P-50 (MW 48,000) ...... 40 Simazine ...... 114,115 Pullulan P-800 (MW 853,000) ...... 40 β-Sitosterol ...... 84 Pyrene ...... 117,118 Sorbic acid ...... 62,67 Pyridoxal HCl ...... 29 Sorbitol ...... 33 Pyridoxine HCl ...... 25,26,28,29,89 Sparteine sulfate ...... 68 Co (II)-1-(2-Pyridylazo)-2-naphthol ...... 124 Stachyose ...... 34 Fe (III)-1-(2-Pyridylazo)-2-naphthol ...... 124 Stevioside ...... 67 Ni (II)-1-(2-Pyridylazo)-2-naphthol ...... 124 Stigmasterol ...... 84 Zn (II)-1-(2-Pyridylazo)-2-naphthol ...... 124 G-Strophanthin ...... 69,85 Pyrimethamine ...... 104 Substance P ...... 51 Pyrogallol ...... 122 Succinic acid ...... 60 Pyrroloquinoline quinone (PQQ) ...... 28 Succinyl-CoA ...... 31 Pyruvic acid ...... 60 Sucrose ...... 32,34 Sugar Alcohols ...... 33 Sulfachloropyridazine ...... 103 Q Sulfadiazine ...... 103,104,105 Sulfadimethoxine ...... 103,104 Sulfadimidine ...... 104,105 Quercetin ...... 71 Sulfadoxine ...... 103 Quinidine ...... 94,97 Sulfaguanidine ...... 103,104 Quinine ...... 68 Sulfamerazine ...... 103,104,105 8-Quinolinol ...... 124 Sulfamethazine ...... 103,104 Sulfamethizole ...... 103,104 Sulfamethoxazole ...... 103,105 R Sulfamethoxypyridazine ...... 103,104 Sulfamonomethoxine ...... 103,104,105 Sulfanilamide ...... 103,104 Raffinose ...... 34 Sulfapyridine ...... 103,104

Sulfaquinoxaline sodium ...... 103 L-Tryptophan ...... 48 Sulfathiazole ...... 103,104,105 5'-TTP ...... 45 Sulfisomidine ...... 103,104 Tyrosine ...... 48 Sulfisoxazole ...... 103 L-Tyrosine ...... 48,49 Sulindac ...... 91 Sunset Yellow FCF53, ...... 65 Swep ...... 115 U

T 5'-UDP ...... 45 UDP-D-glucose ...... 47 UDP-N-acetyl-D-glucosamine ...... 115,123 Tartaric acid ...... 60 5'-UMP ...... 45,463 Tartrazine ...... 65 Unsaturated chloro-disaccharides ...... 33 Taurine ...... 48,49 Uracil ...... 43,44,96,98,124 5'-TDP ...... 45 Urea ...... 123 Terbumeton ...... 114,115 Uric acid ...... 99,101 Terbuthylazine ...... 114,115 Uridine ...... 43 Terbutryn ...... 114,115 5'-UTP ...... 45 Terephthalic acid ...... 125 o-Terphenyl ...... 127 m-Terphenyl ...... 127 V p-Terphenyl ...... 127 Testosterone ...... 81,82 Testosterone enanthate ...... 81,82 [Arg8]- Vasopressin ...... 58,59 Testosterone propionate ...... 81,82 n -Valeraldehyde 2,4-DNPH ...... 117 Tetracaine hydrochloride ...... 97 Valine ...... 48 Tetracycline ...... 105 L-Valine ...... 48,49 Tetracycline hydrochloride ...... 113 Vancomycin hydrochloride ...... 113 Theobromine ...... 68 Verapamil HCl ...... 94,97,101 Theophylline ...... 68,98 Vitamin A ...... 21 Thiabendazole ...... 116 Vitamin A palmitate ...... 21

Thiamine HCl ...... 25,26,30 Vitamin B1 ...... 25,26 Thiamphenicol ...... 104 Vitamin B2 (Flavin mononucleotide) ...... 26,27 Threonine ...... 48 Vitamin B2 (Riboflavin) ...... 25,26,27,29 L-Threonine ...... 48,49 Vitamin B6 (Pyridoxal HCl) ...... 29,30 ∆-Threonine ...... 48 Vitamin B6 (Pyridoxine HCl) ...... 25,26,28,29,89 Thymidine ...... 43 Vitamin B12 ...... 25,27 Thymine ...... 43 Vitamin B13 ...... 28 5'-TMP ...... 45 Vitamin C ...... 25,26,28,31

α-Tocopherol ...... 21,23,24,25 Vitamin D2 ...... 21,22 β-Tocopherol ...... 23 Vitamin D3 ...... 21,22 γ-Tocopherol ...... 23,24 Vitamin E ...... 21 δ¬-Tocopherol ...... 21,23 Vitamin H ...... 27

α-Tocopherol acetate ...... 21,24,25 Vitamin K1 ...... 21,25 Tolmetin sodium ...... 91 Vitamin K2 ...... 21,25 p -Tolualdehyde 2,4-DNPH ...... 117 Vitamin K3 ...... 21 Triamcinolone ...... 78-80 Triamcinolone acetonide ...... 78-80 2,4,6-Trichlorophenol ...... 121 X Triethanolamine ...... 123 Trifluoperazine hydrochloride ...... 92 Triglycine ...... 56 Xanthine ...... 23,29,43,99 Trigonerlline ...... 68 Xanthosine ...... 43 2,4,5-Trihydroxy butyrophenone(THBP) ...... 63,64 Xylitol ...... 33 Trimethoprim ...... 103,104 Xylose ...... 32 O -Trimethyl-β-cyclodextrin ...... 38 2,4,6-Trinitrophenol ...... 121 Triphenylene ...... 117,118 Triton X-100 ...... 123 Tryptophan ...... 48

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