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Taxonomy and Phylogenetic Relationships of Nine Species of Hypocrea with Anamorphs Assignable to Trichoderma Section Hypocreanum
STUDIES IN MYCOLOGY 56: 39–65. 2006. doi:10.3114/sim.2006.56.02 Taxonomy and phylogenetic relationships of nine species of Hypocrea with anamorphs assignable to Trichoderma section Hypocreanum Barrie E. Overton1*, Elwin L. Stewart2 and David M. Geiser2 1The Pennsylvania State University, Department of Plant Pathology, Buckhout Laboratory, University Park, Pennsylvania 16802, U.S.A.: Current address: Lock Haven University of Pennsylvania, Department of Biology, 119 Ulmer Hall, Lock Haven PA, 17745, U.S.A.; 2The Pennsylvania State University, Department of Plant Pathology, Buckhout Laboratory, University Park, Pennsylvania 16802, U.S.A. *Correspondence: Barrie E. Overton, [email protected] Abstract: Morphological studies and phylogenetic analyses of DNA sequences from the internal transcribed spacer (ITS) regions of the nuclear ribosomal gene repeat, a partial sequence of RNA polymerase II subunit (rpb2), and a partial sequence of the large exon of tef1 (LEtef1) were used to investigate the taxonomy and systematics of nine Hypocrea species with anamorphs assignable to Trichoderma sect. Hypocreanum. Hypocrea corticioides and H. sulphurea are reevaluated. Their Trichoderma anamorphs are described and the phylogenetic positions of these species are determined. Hypocrea sulphurea and H. subcitrina are distinct species based on studies of the type specimens. Hypocrea egmontensis is a facultative synonym of the older name H. subcitrina. Hypocrea with anamorphs assignable to Trichoderma sect. Hypocreanum formed a well-supported clade. Five species with anamorphs morphologically similar to sect. Hypocreanum, H. avellanea, H. parmastoi, H. megalocitrina, H. alcalifuscescens, and H. pezizoides, are not located in this clade. Protocrea farinosa belongs to Hypocrea s.s. Taxonomic novelties: Hypocrea eucorticioides Overton, nom. -
Ergot Alkaloids Mycotoxins in Cereals and Cereal-Derived Food Products: Characteristics, Toxicity, Prevalence, and Control Strategies
agronomy Review Ergot Alkaloids Mycotoxins in Cereals and Cereal-Derived Food Products: Characteristics, Toxicity, Prevalence, and Control Strategies Sofia Agriopoulou Department of Food Science and Technology, University of the Peloponnese, Antikalamos, 24100 Kalamata, Greece; [email protected]; Tel.: +30-27210-45271 Abstract: Ergot alkaloids (EAs) are a group of mycotoxins that are mainly produced from the plant pathogen Claviceps. Claviceps purpurea is one of the most important species, being a major producer of EAs that infect more than 400 species of monocotyledonous plants. Rye, barley, wheat, millet, oats, and triticale are the main crops affected by EAs, with rye having the highest rates of fungal infection. The 12 major EAs are ergometrine (Em), ergotamine (Et), ergocristine (Ecr), ergokryptine (Ekr), ergosine (Es), and ergocornine (Eco) and their epimers ergotaminine (Etn), egometrinine (Emn), egocristinine (Ecrn), ergokryptinine (Ekrn), ergocroninine (Econ), and ergosinine (Esn). Given that many food products are based on cereals (such as bread, pasta, cookies, baby food, and confectionery), the surveillance of these toxic substances is imperative. Although acute mycotoxicosis by EAs is rare, EAs remain a source of concern for human and animal health as food contamination by EAs has recently increased. Environmental conditions, such as low temperatures and humid weather before and during flowering, influence contamination agricultural products by EAs, contributing to the Citation: Agriopoulou, S. Ergot Alkaloids Mycotoxins in Cereals and appearance of outbreak after the consumption of contaminated products. The present work aims to Cereal-Derived Food Products: present the recent advances in the occurrence of EAs in some food products with emphasis mainly Characteristics, Toxicity, Prevalence, on grains and grain-based products, as well as their toxicity and control strategies. -
Fungal Endophytes from the Aerial Tissues of Important Tropical Forage Grasses Brachiaria Spp
University of Kentucky UKnowledge International Grassland Congress Proceedings XXIII International Grassland Congress Fungal Endophytes from the Aerial Tissues of Important Tropical Forage Grasses Brachiaria spp. in Kenya Sita R. Ghimire International Livestock Research Institute, Kenya Joyce Njuguna International Livestock Research Institute, Kenya Leah Kago International Livestock Research Institute, Kenya Monday Ahonsi International Livestock Research Institute, Kenya Donald Njarui Kenya Agricultural & Livestock Research Organization, Kenya Follow this and additional works at: https://uknowledge.uky.edu/igc Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/23/2-2-1/6 The XXIII International Grassland Congress (Sustainable use of Grassland Resources for Forage Production, Biodiversity and Environmental Protection) took place in New Delhi, India from November 20 through November 24, 2015. Proceedings Editors: M. M. Roy, D. R. Malaviya, V. K. Yadav, Tejveer Singh, R. P. Sah, D. Vijay, and A. Radhakrishna Published by Range Management Society of India This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact [email protected]. Paper ID: 435 Theme: 2. Grassland production and utilization Sub-theme: 2.2. Integration of plant protection to optimise production -
A Preliminary Enumeration of Grass Endophytes in West Central England
ZOBODAT - www.zobodat.at Zoologisch-Botanische Datenbank/Zoological-Botanical Database Digitale Literatur/Digital Literature Zeitschrift/Journal: Sydowia Jahr/Year: 1992 Band/Volume: 44 Autor(en)/Author(s): White jr, J. F., Baldwin N. A. Artikel/Article: A prliminary enumeration of grass endophytes in west central England. 78-84 ©Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.at A preliminary enumeration of grass endophytes in west central England J. F. White, Jr.1 & N. A. Baldwin2 •Department of Biology, Auburn University at Montgomery, Montgomery, Ala- bama 36117, U.S.A. 2The Sports Turf Research Institute, Bingley, West Yorkshire, BD16 1AU, U. K. White, J. F., Jr. & N. A. Baldwin (1992). A preliminary enumeration of grass endophytes in west central England. - Sydowia 44: 78-84. Presence of endophytes was assessed at 10 different sites in central England. Stromata-producing endophytes were commonly encountered in populations of Agrostis capillaris, A. stolonifera, Dactylis glomerata, and Holcus lanatus. Asymp- tomatic endophytes were detected in Bromus ramosus, Festuca arundinacea, F ovina var. hispidula, F. pratensis, F. rubra, and Festulolium loliaceum. Endophytes were isolated from several grasses and identified. Keywords: Acremonium, endophyte, Epichloe typhina, grasses. Over the past several years endophytes related to the ascomycete Epichloe typhina (Pers.) Tul. have been found to be "widespread in predominantly cool season grasses (Clay & Leuchtmann, 1989; Latch & al., 1984; White, 1987). While most of the endophytes do not produce stromata on host grasses, their colonies on agar cultures, conidiogenous cells and often conidia are similar to those of E. typhina (Leuchtmann & Clay, 1990). These cultural expressions of E. -
Review of Oxepine-Pyrimidinone-Ketopiperazine Type Nonribosomal Peptides
H OH metabolites OH Review Review of Oxepine-Pyrimidinone-Ketopiperazine Type Nonribosomal Peptides Yaojie Guo , Jens C. Frisvad and Thomas O. Larsen * Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, Building 221, DK-2800 Kgs. Lyngby, Denmark; [email protected] (Y.G.); [email protected] (J.C.F.) * Correspondence: [email protected]; Tel.: +45-4525-2632 Received: 12 May 2020; Accepted: 8 June 2020; Published: 15 June 2020 Abstract: Recently, a rare class of nonribosomal peptides (NRPs) bearing a unique Oxepine-Pyrimidinone-Ketopiperazine (OPK) scaffold has been exclusively isolated from fungal sources. Based on the number of rings and conjugation systems on the backbone, it can be further categorized into three types A, B, and C. These compounds have been applied to various bioassays, and some have exhibited promising bioactivities like antifungal activity against phytopathogenic fungi and transcriptional activation on liver X receptor α. This review summarizes all the research related to natural OPK NRPs, including their biological sources, chemical structures, bioassays, as well as proposed biosynthetic mechanisms from 1988 to March 2020. The taxonomy of the fungal sources and chirality-related issues of these products are also discussed. Keywords: oxepine; nonribosomal peptides; bioactivity; biosynthesis; fungi; Aspergillus 1. Introduction Nonribosomal peptides (NRPs), mostly found in bacteria and fungi, are a class of peptidyl secondary metabolites biosynthesized by large modularly organized multienzyme complexes named nonribosomal peptide synthetases (NRPSs) [1]. These products are amongst the most structurally diverse secondary metabolites in nature; they exhibit a broad range of activities, which have been exploited in treatments such as the immunosuppressant cyclosporine A and the antibiotic daptomycin [2,3]. -
Ascomyceteorg 08-05 Ascomyceteorg
Chaetomium convolutum and C. hexagonosporum from Italy with a morphological revision of the group-bostrychodes Francesco DOVERI Abstract: Chaetomium convolutum, newly isolated from herbivore dung in a survey of coprophilous asco- mycetes and basidiomycetes, is first described from Italy and compared with other species of the so called “group-bostrychodes”. The taxonomic uncertainty, still present in this group despite a recent comparative, morphological and molecular study, led the author to revise all Italian collections of species related to C. convolutum, and to prove that also the very rare C. hexagonosporum is present in Italy. The morphological Ascomycete.org, 8 (5) : 185-198. features of C. hexagonosporum are described in detail and particularly compared with those of C. convolutum. Novembre 2016 The results of this review confirm the existence of several intermediates in the group-bostrychodes, for which Mise en ligne le 3/11/2016 a much more extended revision is hoped. Keywords: cellulose, Chaetomiun bostrychodes, Chaetomium robustum, coprophilous fungi, morphological study. Riassunto: Chaetomium convolutum, isolato recentemente da escrementi di erbivori in un’ indagine sugli ascomiceti e basidiomiceti coprofili, è descritto per la prima volta in Italia e messo a confronto con altre specie del cosiddetto "gruppo-bostrychodes". L'incertezza tassonomica, ancora presente in questo gruppo nonostante un recente studio comparativo, morfologico e molecolare, ha indotto l'autore a rivedere tutte le raccolte italiane correlate a C. convolutum, e a dimostrare che anche il molto raro C. hexagonosporum è presente in Italia. Le caratteristiche morfologiche di C. hexagonosporum sono dettagliatamente descritte e in modo particolare confrontate con quelle di C. convolutum. -
An Unusual Haemoid Fungi: Ulocladium, As a Cause Of
Volume 2 Number 2 (June 2010) 95-97 CASE Report An unusual phaeoid fungi: Ulocladium, as a cause of chronic allergic fungal sinusitis Kaur R, Wadhwa A1,Gulati A2, Agrawal AK2 1Department of Microbiology. 2Department of Otorhinolaryngology, Maulana Azad Medical College, New Delhi, India. Received: April 2010, Accepted: May 2010. ABSTRACT Allergic fungal sinusitis (AFS) has been recognized as an important cause of chronic sinusitis commonly caused by Aspergillus spp. and various dematiaceous fungi like Bipolaris, Alternaria, Curvalaria, and etc. Ulocladium botrytis is a non pathogenic environmental dematiaceous fungi, which has been recently described as a human pathogen. Ulocladium has never been associated with allergic fungal sinusitis but it was identified as an etiological agent of AFS in a 35 year old immunocompetent female patient presenting with chronic nasal obstruction of several months duration to our hospital. The patient underwent FESS and the excised polyps revealed Ulocladium as the causative fungal agent. Keywords: Ulocladium, Phaeoid Fungi, Chronic Sinusitis. INTRODUCTION CASE REPORT Chronic allergic sinusitis is a common condition A 35 year old female patient presented to the ENT responsible for the development of nasal polyps, out patient department of the Lok Nayak Hospital, described as abnormal lesions that emanate from Delhi, with chronic nasal obstruction, excessive any portion of the nasal mucosa or Para nasal sneezing, nasal discharge and frontal headache since sinuses. They are commonly located in the middle several months. Nasal obstruction was of gradual meatus and ethmoid sinus and are present in onset, non progressive, more on the left side than 1-4% of the population (1). -
Isolation of Fungal Cellulase Gene Transcript from Penicillium Spinulosum
Isolation of fungal cellulase gene transcript from Penicillium spinulosum A Master’s Thesis Presented to the faculty of The College of Science and Mathematics Colorado State University – Pueblo In Partial Fulfillment of the requirements for the degree of Master of Science in Biochemistry By Srivatsan Parthasarathy Colorado State University – Pueblo May, 2018 ACKNOWLEDGEMENTS I would like to thank my research mentor Dr. Sandra Bonetti for guiding me through my research thesis and helping me in difficult times during my Master’s degree. I would like to thank Dr. Dan Caprioglio for helping me plan my experiments and providing the lab space and equipment. I would like to thank the department of Biology and Chemistry for supporting me through assistantships and scholarships. I would like to thank my wife Vaishnavi Nagarajan for the emotional support that helped me complete my degree at Colorado State University – Pueblo. III TABLE OF CONTENTS 1) ACKNOWLEDGEMENTS …………………………………………………….III 2) TABLE OF CONTENTS …………………………………………………….....IV 3) ABSTRACT……………………………………………………………………..V 4) LIST OF FIGURES……………………………………………………………..VI 5) LIST OF TABLES………………………………………………………………VII 6) INTRODUCTION………………………………………………………………1 7) MATERIALS AND METHODS………………………………………………..24 8) RESULTS………………………………………………………………………..50 9) DISCUSSION…………………………………………………………………….77 10) REFERENCES…………………………………………………………………...99 11) THESIS PRESENTATION SLIDES……………………………………………...113 IV ABSTRACT Cellulose and cellulosic materials constitute over 85% of polysaccharides in landfills. Cellulose is also the most abundant organic polymer on earth. Cellulose digestion yields simple sugars that can be used to produce biofuels. Cellulose breaks down to form compounds like hemicelluloses and lignins that are useful in energy production. Industrial cellulolysis is a process that involves multiple acidic and thermal treatments that are harsh and intensive. -
New Record of Chaetomium Species Isolated from Soil Under Pineapple Plantation in Thailand
Journal of Agricultural Technology 2008, V.4(2): 91-103 New record of Chaetomium species isolated from soil under pineapple plantation in Thailand C. Pornsuriya1*, F.C. Lin2, S. Kanokmedhakul3and K. Soytong1 1Department of Plant Pest Management Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang (KMITL), Bangkok 10520, Thailand. 2College of Agriculture and Biotechnology, Biotechnology Institute, Zhejiang University, Kaixuan Road, Hangzhou 310029, P.R. China. 3Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand. Pornsuriya, C., Lin, F.C., Kanokmedhakul, S. and Soytong, K. (2008). New record of Chaetomium species isolated from soil under pineapple plantation in Thailand. Journal of Agricultural Technology 4(2): 91-103. Chaetomium species were isolated from soil in pineapple plantations in Phatthalung and Rayong provinces by soil plate and baiting techniques. Taxonomic study was based on available dichotomously keys and monograph of the genus. Five species are recorded as follows: C. aureum, C. bostrychodes, C. cochliodes, C. cupreum and C. gracile. Another four species are reported to be new records in Thailand as follows: C. carinthiacum, C. flavigenum, C. perlucidum and C. succineum. Key words: Chaetomium, Taxonomic study Introduction Chaetomium is a fungus belonging to Ascomycota of the family Chaetomiaceae which established by Kunze in 1817 (von Arx et al., 1986). Chaetomium Kunze is one of the largest genera of saprophytic ascomycetes which comprise more than 300 species worldwide (von Arx et al., 1986; Soytong and Quimio, 1989; Decock and Hennebert, 1997; Udagawa et al., 1997; Rodríguez et al., 2002). Approximately 20 species have been recorded in Thailand (Table 1). -
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Isolation and Identification of Cold-Adapted Fungi in the Fox Permafrost Tunnel, Alaska Mark P. Waldrop United States Geological Survey, Geologic Division, Menlo Park, CA, USA Richard White III United States Geological Survey, Geologic Division, Menlo Park, CA, USA Thomas A. Douglas Cold Regions Research and Engineering Laboratory, Fort Wainwright, AK, USA Abstract Permafrost microbiology is important for understanding biogeochemical processes, paleoecology, and life in extreme environments. Within the Fox, Alaska, permafrost tunnel, fungi grow on tunnel walls despite below freezing (-3°C) temperatures for the past 15,000 years. We collected fungal mycelia from ice, Pleistocene roots, and frozen loess. We identified the fungi by PCR, amplifying the ITS region of rRNA and searching for related sequences. The fungi within the tunnel were predominantly one genus, Geomyces, a cold-adapted fungi, and has likely “contaminated” the permafrost tunnel from outside. We were unable to obtain DNA or fungal isolates from the frozen loess, indicating fungal survival in permafrost soils can be strongly restricted. Geomyces can degrade complex carbon compounds, but we are unable to determine whether this is occurring. Results from this study suggest Geomyces may be an important colonizer species of other permafrost environments. Keywords: Fox tunnel; fungi; Geomyces; ice wedge; loess; permafrost. Introduction starts to melt and then sublimate. Therefore, when a hole is drilled, moisture is liberated, and fungal growth at these sites The permafrost tunnel near Fox, Alaska, was constructed should be possible. in the early 1960s to examine mining, tunneling, and Our research objective was to determine the identity of the construction techniques in permafrost. -
DNA Barcoding of Fungi in the Forest Ecosystem of the Psunj and Papukissn Mountains 1847-6481 in Croatia Eissn 1849-0891
DNA Barcoding of Fungi in the Forest Ecosystem of the Psunj and PapukISSN Mountains 1847-6481 in Croatia eISSN 1849-0891 OrIGINAL SCIENtIFIC PAPEr DOI: https://doi.org/10.15177/seefor.20-17 DNA barcoding of Fungi in the Forest Ecosystem of the Psunj and Papuk Mountains in Croatia Nevenka Ćelepirović1,*, Sanja Novak Agbaba2, Monika Karija Vlahović3 (1) Croatian Forest Research Institute, Division of Genetics, Forest Tree Breeding and Citation: Ćelepirović N, Novak Agbaba S, Seed Science, Cvjetno naselje 41, HR-10450 Jastrebarsko, Croatia; (2) Croatian Forest Karija Vlahović M, 2020. DNA Barcoding Research Institute, Division of Forest Protection and Game Management, Cvjetno naselje of Fungi in the Forest Ecosystem of the 41, HR-10450 Jastrebarsko; (3) University of Zagreb, School of Medicine, Department of Psunj and Papuk Mountains in Croatia. forensic medicine and criminology, DNA Laboratory, HR-10000 Zagreb, Croatia. South-east Eur for 11(2): early view. https://doi.org/10.15177/seefor.20-17. * Correspondence: e-mail: [email protected] received: 21 Jul 2020; revised: 10 Nov 2020; Accepted: 18 Nov 2020; Published online: 7 Dec 2020 AbStract The saprotrophic, endophytic, and parasitic fungi were detected from the samples collected in the forest of the management unit East Psunj and Papuk Nature Park in Croatia. The disease symptoms, the morphology of fruiting bodies and fungal culture, and DNA barcoding were combined for determining the fungi at the genus or species level. DNA barcoding is a standardized and automated identification of species based on recognition of highly variable DNA sequences. DNA barcoding has a wide application in the diagnostic purpose of fungi in biological specimens. -
Cladosporium Lebrasiae, a New Fungal Species Isolated from Milk Bread Rolls in France
fungal biology 120 (2016) 1017e1029 journal homepage: www.elsevier.com/locate/funbio Cladosporium lebrasiae, a new fungal species isolated from milk bread rolls in France Josiane RAZAFINARIVOa, Jean-Luc JANYa, Pedro W. CROUSb, Rachelle LOOTENa, Vincent GAYDOUc, Georges BARBIERa, Jerome^ MOUNIERa, Valerie VASSEURa,* aUniversite de Brest, EA 3882, Laboratoire Universitaire de Biodiversite et Ecologie Microbienne, ESIAB, Technopole^ Brest-Iroise, 29280 Plouzane, France bCBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands cMeDIAN-Biophotonique et Technologies pour la Sante, Universite de Reims Champagne-Ardenne, FRE CNRS 3481 MEDyC, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims cedex, France article info abstract Article history: The fungal genus Cladosporium (Cladosporiaceae, Dothideomycetes) is composed of a large Received 12 February 2016 number of species, which can roughly be divided into three main species complexes: Cla- Received in revised form dosporium cladosporioides, Cladosporium herbarum, and Cladosporium sphaerospermum. The 29 March 2016 aim of this study was to characterize strains isolated from contaminated milk bread rolls Accepted 15 April 2016 by phenotypic and genotypic analyses. Using multilocus data from the internal transcribed Available online 23 April 2016 spacer ribosomal DNA (rDNA), partial translation elongation factor 1-a, actin, and beta- Corresponding Editor: tubulin gene sequences along with Fourier-transform infrared (FTIR) spectroscopy and Matthew Charles Fisher morphological observations, three isolates were identified as a new species in the C. sphaer- ospermum species complex. This novel species, described here as Cladosporium lebrasiae,is Keywords: phylogenetically and morphologically distinct from other species in this complex. Cladosporium sphaerospermum ª 2016 British Mycological Society.