bioRxiv preprint doi: https://doi.org/10.1101/645150; this version posted May 22, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 RESEARCH PAPER 2 Anaesthesia with diethyl ether impairs jasmonate signalling in the 3 carnivorous plant Venus flytrap (Dionaea muscipula). 4 Andrej Pavlovič1*, Michaela Libiaková2, Boris Bokor2,3, Jana Jakšová1, Ivan Petřík4, Ondřej 5 Novák4, František Baluška5 6 1 Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural 7 Research, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71, Olomouc, Czech 8 Republic 9 2 Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in 10 Bratislava, Ilkovičova 6, Mlynská dolina B2, SK-842 15, Bratislava, Slovakia 11 3 Comenius University Science Park, Comenius University in Bratislava, Ilkovičova 8, SK-841 12 04, Bratislava, Slovakia 13 4 Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and 14 Agricultural Research, Institute of Experimental Botany CAS and Faculty of Science, Palacký 15 University, Šlechtitelů 27, CZ-783 71, Olomouc, Czech Republic 16 5IZMB, University of Bonn, Kirschallee 1, 53115 Bonn, Germany 17 *- author for correspondence:
[email protected], +420 585 634 831 18 Running title: Anaesthetic impairs jasmonate signalling in carnivorous plant 19 Highlight: Carnivorous plant Venus flytrap (Dionaea muscipula) is unresponsive to insect 20 prey or herbivore attack due to impaired electrical and jasmonate signalling under general 21 anaesthesia induced by diethyl ether.