A Comparative Study of Anaesthetic Agents on High Voltage Activated Calcium
bioRxiv preprint doi: https://doi.org/10.1101/2020.12.17.423182; this version posted December 18, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. A COMPARATIVE STUDY OF ANAESTHETIC AGENTS ON HIGH VOLTAGE ACTIVATED CALCIUM CHANNEL CURRENTS IN IDENTIFIED MOLLUSCAN NEURONS Terrence J. Morris1, Philip M. Hopkins2,3 and William Winlow4,5 1Department Science and Technology - Biology, Douglas College, 700 Ryal Avenue, New Westminster, British Columbia, Canada; 2 Leeds Institute of Medical Research at St James’s, School of Medicine, University of Leeds, Leeds, United Kingdom; 3Malignant Hyperthermia Investigation Unit, Leeds Institute of Molecular Medicine, St. James’s University Hospital, Leeds, LS9 7TF, United Kingdom; 4 Department of Biology, University of Naples Federico II, Via Cintia 26, 80126, Naples, Italy; 5Institute of Ageing and Chronic Diseases, University of Liverpool, Liverpool, United Kingdom. Corresponding author: William Winlow Key Words: General anaesthetic, calcium channels, Lymnaea, light yellow cells SUMMARY 1. Using the two electrode voltage clamp configuration, a high voltage activated whole-cell Ca2+ channel current (IBa) was recorded from a cluster of neurosecretory ‘Light Yellow’ Cells (LYC) in the right parietal ganglion of the pond snail Lymnaea stagnalis. 2. Recordings of IBa from LYCs show a reversible concentration-dependent depression of current amplitude in the presence of the volatile anaesthetics halothane, isoflurane and sevoflurane, or the non-volatile anaesthetic pentobarbitone at clinical concentrations. 3. In the presence of the anaesthetics investigated, IBa measured at the end of the depolarizing test pulse showed proportionally greater depression than that at measured peak amplitude, as well as significant decrease in the rate of activation or increase in inactivation or both.
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