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Training Manual Series No.15/2018
DBTR-H D Indian Council of Agricultural Research Ministry of Science and Technology Central Marine Fisheries Research Institute Department of Biotechnology CMFRI Training Manual Series No.15/2018 Training Manual In the frame work of the project: DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals 2015-18 Training Manual In the frame work of the project: DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals 2015-18 Training Manual This is a limited edition of the CMFRI Training Manual provided to participants of the “DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals” organized by the Marine Biotechnology Division of Central Marine Fisheries Research Institute (CMFRI), from 2nd February 2015 - 31st March 2018. Principal Investigator Dr. P. Vijayagopal Compiled & Edited by Dr. P. Vijayagopal Dr. Reynold Peter Assisted by Aditya Prabhakar Swetha Dhamodharan P V ISBN 978-93-82263-24-1 CMFRI Training Manual Series No.15/2018 Published by Dr A Gopalakrishnan Director, Central Marine Fisheries Research Institute (ICAR-CMFRI) Central Marine Fisheries Research Institute PB.No:1603, Ernakulam North P.O, Kochi-682018, India. 2 Foreword Central Marine Fisheries Research Institute (CMFRI), Kochi along with CIFE, Mumbai and CIFA, Bhubaneswar within the Indian Council of Agricultural Research (ICAR) and Department of Biotechnology of Government of India organized a series of training programs entitled “DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals”. The scope of this training is to promote development of trained human resource for application of molecular tools to research problems in fisheries and aquaculture, to help them adapt to such facilities and work programs and to include analyses that comply with worldwide regulatory acts in the field of biotechnology. -
Chaetodon Larvatus Ordine Perciformes Cuvier, 1831 Famiglia Chaetodontidae
Identificazione e distribuzione nei mari italiani di specie non indigene Classe Osteichthyes Chaetodon larvatus Ordine Perciformes Cuvier, 1831 Famiglia Chaetodontidae SINONIMI RILEVANTI Chaetodon karraf Cuvier, 1831 DESCRIZIONE COROLOGIA / AFFINITA’ Corpo fortemente appiattito e alto. Testa piccola Tropicale con bocca protrattile leggermente obliqua. Denti DISTRIBUZIONE ATTUALE lunghi e stretti sulla parte anteriore di entrambe le mascelle. Occhi relativamente grandi, interorbitale Oceano Indiano occidentale: Mar Rosso e Golfo di stretto. Pinna dorsale continua. Pinna caudale Aden. tronca. Piccole scaglie ctenoidi su tutto il corpo. Scaglia ascellare appuntita alla base della pinna PRIMA SEGNALAZIONE IN MEDITERRANEO pelvica. Bordo del preopercolo denticolato. Israele, gennaio 2011 (Salameh et al. 2011) COLORAZIONE PRIMA SEGNALAZIONE IN ITALIA Testa anteriormente marrone-arancione. Corpo - grigiastro con diverse linee giallastre verticali a V. Parte posteriore della pinna dorsale nera; pinna ORIGINE caudale nera con bordo posteriore bianco- Mar Rosso trasparente. Pinna anale grigia. Pinna pettorale trasparente. Pinna pelvica arancione. VIE DI DISPERSIONE PRIMARIE Migrazione lessepsiana. FORMULA MERISTICA D XI,27; A III,23; P 15; V I,5 Identificazione e distribuzione nei mari italiani di specie non indigene TAGLIA MASSIMA VIE DI DISPERSIONE SECONDARIE 120 mm STADI LARVALI STATO DELL ’INVASIONE Alieno. SPECIE SIMILI MOTIVI DEL SUCCESSO CARATTERI DISTINTIVI La tipica colorazione distingue questa specie dalle altre specie appartenenti alla -
Growth of Chaetodon Larvatus (Chaetodontidae: Pisces) in the Southern Red Sea
Marine Biology (2006) 148: 1113–1122 DOI 10.1007/s00227-005-0146-7 RESEARCH ARTICLE Z. A. Zekeria Æ S. Weertman Æ B. Samuel Æ T. Kale-ab J. J. Videler Growth of Chaetodon larvatus (Chaetodontidae: Pisces) in the southern Red Sea Received: 22 March 2004 / Accepted: 15 August 2005 / Published online: 15 November 2005 Ó Springer-Verlag 2005 Abstract Growth and age of Chaetodon larvatus were vertebrae to estimate age while the second is based on studied using growth bands in otoliths and length-fre- the length distribution of fish in a cohort and monitors quency analyses. Otoliths of 180 C. larvatus were ex- changes in the distribution with time. Both methods tracted and measured. Polished sections of sagittae have been widely employed for growth and ageing revealed alternating opaque and translucent bands cor- studies of temperate fishes and yielded good results. responding with a seasonal growth pattern. Both mass Until recently, the methods were not used for tropical and size of the otoliths continue to grow steadily fish growth studies for two reasons. First, tropical fish throughout life. Length-at-age data revealed very fast were assumed to lack seasonal growth patterns. This was growth during the first year. Growth proceeded at a thought to result in poorly developed growth marks in decreasing rate during the second and the third year; the hard parts (Brothers 1980). Second, tropical fishes fishes older than 3 years did not grow noticeably. No were believed to lack seasonality in recruitment. Pro- difference in growth patterns between males and females tracted recruitment would result in skewed and bimodal could be detected. -
Co-Evolution of Cleaning and Feeding Morphology in Western Atlantic and Eastern Pacific Gobies
ORIGINAL ARTICLE doi:10.1111/evo.13904 Co-evolution of cleaning and feeding morphology in western Atlantic and eastern Pacific gobies Jonathan M. Huie,1,2 Christine E. Thacker,3,4 and Luke Tornabene1,5 1School of Aquatic and Fishery Sciences, University of Washington, 1122 NE Boat St, Seattle, Washington 98195 2E-mail: [email protected] 3Santa Barbara Museum of Natural History, 2559 Puesta del Sol, Santa Barbara, California 93105 4Natural History Museum of Los Angeles County, 900 Exposition Blvd, Los Angeles, California 90007 5Burke Museum of Natural History and Culture, 4300 15th Ave NE, Seattle, Washington 98105 Received September 2, 2019 Accepted November 25, 2019 Cleaning symbioses are mutualistic relationships where cleaners remove and consume ectoparasites from their clients. Cleaning behavior is rare in fishes and is a highly specialized feeding strategy only observed in around 200 species. Cleaner fishes vary in their degree of specialization, ranging from species that clean as juveniles or facultatively as adults, to nearly obligate or dedicated cleaners. Here, we investigate whether these different levels of trophic specialization correspond with similar changes in feeding morphology. Specifically, we model the evolution of cleaning behavior across the family Gobiidae, which contains the most speciose radiation of dedicated and facultative cleaner fishes. We compared the cranial morphology and dentition of cleaners and non-cleaners across the phylogeny of cleaning gobies and found that facultative cleaners independently evolved four times and have converged on an intermediate morphology relative to that of dedicated cleaners and non-cleaning generalists. This is consistent with their more flexible feeding habits. Cleaner gobies also possess a distinct tooth morphology, which suggests they are adapted for scraping parasites off their clients and show little similarity to other cleaner clades. -
The Genome of the Gulf Pipefish Enables Understanding of Evolutionary Innovations C
Small et al. Genome Biology (2016) 17:258 DOI 10.1186/s13059-016-1126-6 RESEARCH Open Access The genome of the Gulf pipefish enables understanding of evolutionary innovations C. M. Small1†, S. Bassham1†, J. Catchen1,2†, A. Amores3, A. M. Fuiten1, R. S. Brown1,4, A. G. Jones5 and W. A. Cresko1* Abstract Background: Evolutionary origins of derived morphologies ultimately stem from changes in protein structure, gene regulation, and gene content. A well-assembled, annotated reference genome is a central resource for pursuing these molecular phenomena underlying phenotypic evolution. We explored the genome of the Gulf pipefish (Syngnathus scovelli), which belongs to family Syngnathidae (pipefishes, seahorses, and seadragons). These fishes have dramatically derived bodies and a remarkable novelty among vertebrates, the male brood pouch. Results: We produce a reference genome, condensed into chromosomes, for the Gulf pipefish. Gene losses and other changes have occurred in pipefish hox and dlx clusters and in the tbx and pitx gene families, candidate mechanisms for the evolution of syngnathid traits, including an elongated axis and the loss of ribs, pelvic fins, and teeth. We measure gene expression changes in pregnant versus non-pregnant brood pouch tissue and characterize the genomic organization of duplicated metalloprotease genes (patristacins) recruited into the function of this novel structure. Phylogenetic inference using ultraconserved sequences provides an alternative hypothesis for the relationship between orders Syngnathiformes and Scombriformes. Comparisons of chromosome structure among percomorphs show that chromosome number in a pipefish ancestor became reduced via chromosomal fusions. Conclusions: The collected findings from this first syngnathid reference genome open a window into the genomic underpinnings of highly derived morphologies, demonstrating that de novo production of high quality and useful reference genomes is within reach of even small research groups. -
And Platycephalus Indicus (Teleostei: Platycephalidae) in the Mediterranean Sea
BioInvasions Records (2012) Volume 1, Issue 1: 53–57 doi: http://dx.doi.org/10.3391/bir.2012.1.1.12 Open Access © 2012 The Author(s). Journal compilation © 2012 REABIC Aquatic Invasions Records Recent evidence on the presence of Heniochus intermedius (Teleostei: Chaetodontidae) and Platycephalus indicus (Teleostei: Platycephalidae) in the Mediterranean Sea Michel Bariche Department of Biology, Faculty of Arts and Sciences, American University of Beirut, PO Box 11-0236, Beirut, Lebanon E-mail: [email protected] Received: 4 January 2012 / Accepted: 23 February 2012 / Published online: 7 March 2012 Handling editor: Ernesto Azzurro, ISPRA, Institute for Environmental Protection and Research, Italy Abstract A second specimen of the Red Sea bannerfish Heniochus intermedius Steindachner, 1893 and a specimen of the Bartail flathead Platycephalus indicus (Linnaeus, 1758) have been recently collected from Lebanon (eastern Mediterranean). The two alien species constitute very rare occurrences in the Mediterranean; the first record of H. intermedius dates back to 2002 and only a few P. indicus individuals were collected between the 1950s and 1970s. Their presence in the Mediterranean is discussed as well as possible future trends in light of recent environmental changes. Key words: Heniochus intermedius, Platycephalus indicus, alien species, Lessepsian migration, Lebanon, eastern Mediterranean Introduction associated to coral reefs (Randall 1983; CIESM 2009). Butterflyfishes (Chaetodontidae) are marine Flatheads (Platycephalidae) are large bottom fishes that can be easily recognized by a deep dwelling fishes found mostly in the Indo-Pacific compressed body, small terminal and protractile area. They are characterized by an elongate mouth and bright coloration patterns (Randall body, a depressed head and a large mouth, with 1983; Nelson 2006). -
Volume 19 Winter 2002 the Coral Hind, Lapu Lapu, Or Miniata
FREE ISSN 1045-3520 Volume 19 Winter 2002 Introducing a Zonal Based Natural Photo by Robert Fenner Filtration System for Reef Aquariums by Steve Tyree Quite a few natural based filtration systems have been devised by reef aquarists and scientists in the past twenty years. Some systems utilized algae to remove organic and inorganic pollutants from the reef aquarium; others utilized sediment beds. The natural filtration system that I have been researching and designing is drastically different from both of these types. No external algae are used. I believe that all the algae a functional reef requires are already growing in the reef, even if they are not apparent. They include micro-algae, turf algae, coralline algae, single-cell algae within photosynthetic corals, and cyanobacteria with photosynthetic capabilities. Most of the systems that I have set up to research this concept have not included sediment beds. All organic matter and pollutants are recycled and processed within the system by macro-organisms. Sediment beds have not been utilized to process excess Miniata Grouper, Cephalopholis miniata organic debris, but that does not prevent other aquarists from adding them. The main concept behind my system is the use of living sponges, sea squirts, and filter feeders for filtration. Sponges consume bacteria, can reach about twenty inches in length in the wild, and dissolved and colloidal organic material, micro-plankton, The Coral Hind, Lapu about half that in captivity. It is undoubtedly the most and fine particulate matter. Sea squirts consume large Lapu, or Miniata prized member of the genus for the aquarium trade. -
Download Fishlore.Com's Saltwater Aquarium and Reef Tank E-Book
Updated: August 6, 2013 This e-Book is FREE for public use. Commercial use prohibited. Copyright FishLore.com – providing tropical fish tank and aquarium fish information for freshwater fish and saltwater fish keepers. FishLore.com Saltwater Aquarium & Reef Tank e-Book 1 CONTENTS Foreword .......................................................................................................................................... 10 Why Set Up an Aquarium? .............................................................................................................. 12 Aquarium Types ............................................................................................................................... 14 Aquarium Electrical Safety ............................................................................................................... 15 Aquarium Fish Cruelty Through Ignorance ..................................................................................... 17 The Aquarium Nitrogen Cycle ......................................................................................................... 19 Aquarium Filter and Fish Tank Filtration ......................................................................................... 24 Saltwater Aquarium Types - FOWLR, Fish Only with Live Rock, Reef Tank .................................... 30 Freshwater Aquarium vs. Saltwater Aquarium ............................................................................... 33 Saltwater Aquarium Tank Setup Guide .......................................................................................... -
Hotspots, Extinction Risk and Conservation Priorities of Greater Caribbean and Gulf of Mexico Marine Bony Shorefishes
Old Dominion University ODU Digital Commons Biological Sciences Theses & Dissertations Biological Sciences Summer 2016 Hotspots, Extinction Risk and Conservation Priorities of Greater Caribbean and Gulf of Mexico Marine Bony Shorefishes Christi Linardich Old Dominion University, [email protected] Follow this and additional works at: https://digitalcommons.odu.edu/biology_etds Part of the Biodiversity Commons, Biology Commons, Environmental Health and Protection Commons, and the Marine Biology Commons Recommended Citation Linardich, Christi. "Hotspots, Extinction Risk and Conservation Priorities of Greater Caribbean and Gulf of Mexico Marine Bony Shorefishes" (2016). Master of Science (MS), Thesis, Biological Sciences, Old Dominion University, DOI: 10.25777/hydh-jp82 https://digitalcommons.odu.edu/biology_etds/13 This Thesis is brought to you for free and open access by the Biological Sciences at ODU Digital Commons. It has been accepted for inclusion in Biological Sciences Theses & Dissertations by an authorized administrator of ODU Digital Commons. For more information, please contact [email protected]. HOTSPOTS, EXTINCTION RISK AND CONSERVATION PRIORITIES OF GREATER CARIBBEAN AND GULF OF MEXICO MARINE BONY SHOREFISHES by Christi Linardich B.A. December 2006, Florida Gulf Coast University A Thesis Submitted to the Faculty of Old Dominion University in Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE BIOLOGY OLD DOMINION UNIVERSITY August 2016 Approved by: Kent E. Carpenter (Advisor) Beth Polidoro (Member) Holly Gaff (Member) ABSTRACT HOTSPOTS, EXTINCTION RISK AND CONSERVATION PRIORITIES OF GREATER CARIBBEAN AND GULF OF MEXICO MARINE BONY SHOREFISHES Christi Linardich Old Dominion University, 2016 Advisor: Dr. Kent E. Carpenter Understanding the status of species is important for allocation of resources to redress biodiversity loss. -
Review of the Western Atlantic Species of Bollmannia (Teleostei: Gobiidae: Gobiosomatini) with the Description of a New Allied Genus and Species
aqua, International Journal of Ichthyology Review of the western Atlantic species of Bollmannia (Teleostei: Gobiidae: Gobiosomatini) with the description of a new allied genus and species James L. Van Tassell1*, Luke Tornabene2, Patrick L. Colin3 1) American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024-5192, U.S.A. *Corresponding author: [email protected] 2) Texas A&M University – Corpus Christi, 6300 Ocean Drive, Corpus Christi, TX 78412, U.S.A. 3) Coral Reef Research Foundation P.O. Box 1765 Koror, Palau 96940 Received: 20 May 2011 – Accepted: 30 September 2011 Abstract Résumé Bollmannia Jordan is a poorly studied group of American Bollmannia Jordan est un groupe peu étudié de gobies seven-spined gobies with representatives in the tropical américains à sept épines avec des représentants dans l’Atlan- and subtropical western Atlantic and tropical eastern Pa - tique ouest tropical et subtropical et dans le Pacifique est cific oceans. We review the taxonomy of the western tropical. Nous faisons une révision de espèces de l’Atlantique Atlantic species and provide redescriptions for the four ouest et donnons la redescription des quatre espèces recon- valid species: B. boqueronensis, B. communis, B. eigenmanni nues : B. boqueronensis, B. communis, B. eigenmanni et B. and B. litura. Bollmannia jeannae is considered to be a litura. Bollmannia jeannae est considéré comme un syn- junior synonym of B. boqueronensis. We also describe a onyme plus récent de B. boqueronensis. Nous décrivons aussi new genus and species of deep-water goby and discuss its un nouveau genre et une nouvelle espèce de gobie des eaux affinities to Bollmannia and other genera of the Micro - profondes et en discutons les affinités avec Bollmannia et gobius group of the Gobiosomatini. -
Assessment of the Flame Angelfish (Centropyge Loriculus) As a Model Species in Studies on Egg and Larval Quality in Marine Fishes Chatham K
The University of Maine DigitalCommons@UMaine Electronic Theses and Dissertations Fogler Library 8-2007 Assessment of the Flame Angelfish (Centropyge loriculus) as a Model Species in Studies on Egg and Larval Quality in Marine Fishes Chatham K. Callan Follow this and additional works at: http://digitalcommons.library.umaine.edu/etd Part of the Aquaculture and Fisheries Commons, and the Oceanography Commons Recommended Citation Callan, Chatham K., "Assessment of the Flame Angelfish (Centropyge loriculus) as a Model Species in Studies on Egg and Larval Quality in Marine Fishes" (2007). Electronic Theses and Dissertations. 126. http://digitalcommons.library.umaine.edu/etd/126 This Open-Access Dissertation is brought to you for free and open access by DigitalCommons@UMaine. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of DigitalCommons@UMaine. ASSESSMENT OF THE FLAME ANGELFISH (Centropyge loriculus) AS A MODEL SPECIES IN STUDIES ON EGG AND LARVAL QUALITY IN MARINE FISHES By Chatham K. Callan B.S. Fairleigh Dickinson University, 1997 M.S. University of Maine, 2000 A THESIS Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy (in Marine Biology) The Graduate School The University of Maine August, 2007 Advisory Committee: David W. Townsend, Professor of Oceanography, Advisor Linda Kling, Associate Professor of Aquaculture and Fish Nutrition, Co-Advisor Denise Skonberg, Associate Professor of Food Science Mary Tyler, Professor of Biological Science Christopher Brown, Professor of Marine Science (Florida International University) LIBRARY RIGHTS STATEMENT In presenting this thesis in partial fulfillment of the requirements for an advanced degree at The University of Maine, I agree that the Library shall make it freely available for inspection. -
Development of Larval Fish Rearing Techniques and Nutrient Requirements for the Green Mandarin, Synchiropus Splendidus: a Popular Marine Ornamental Fish
ResearchOnline@JCU This file is part of the following reference: Shao, Luchang (2016) Development of larval fish rearing techniques and nutrient requirements for the green mandarin, Synchiropus splendidus: a popular marine ornamental fish. PhD thesis, James Cook University. Access to this file is available from: http://researchonline.jcu.edu.au/47308/ The author has certified to JCU that they have made a reasonable effort to gain permission and acknowledge the owner of any third party copyright material included in this document. If you believe that this is not the case, please contact [email protected] and quote http://researchonline.jcu.edu.au/47308/ Development of larval fish rearing techniques and nutrient requirement for the green mandarin, Synchiropus splendidus: a popular marine ornamental fish Thesis submitted by Luchang Shao (MSc) in September 2016 For the degree of Doctor of Philosophy In the College of Marine and Environmental Science James Cook University Declaration on Ethics The research presented and reported in this thesis was conducted within the guidelines for research ethics outlined in the National Statement on Ethics Conduct in Research Involving Human (1999), the Joint NHMRC/AVCC Statement and Guidelines on Research Practice (1997), the James Cook University Policy on Experimentation Ethics Standard Practices and Guidelines (2001), and the James Cook University Statement and Guidelines on Research Practice (2001). The proposed research methodology received clearance from the James Cook University Experimentation Ethics Review Committee. Approval numbers: A1851; Principal investigator: Luchang Shao; Finish date: September 30, 2015 i Statement of contribution of others Financial support for this study was provided by Graduate Research School of James Cook University, JCU Postgraduate Research Scholarship.