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Fetal Hemoglobin in Sickle Cell Anemia: Genetic Determinants of Response to Hydroxyurea

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Fetal Hemoglobin in Sickle Cell Anemia: Genetic Determinants of Response to Hydroxyurea The Pharmacogenomics Journal (2007) 7, 386–394 & 2007 Nature Publishing Group All rights reserved 1470-269X/07 $30.00 www.nature.com/tpj ORIGINAL ARTICLE Fetal hemoglobin in sickle cell anemia: genetic determinants of response to hydroxyurea QMa1, DF Wyszynski1, The increase in fetal hemoglobin (HbF) in response to hydroxyurea (HU) 1 2 1 varies among patients with sickle cell anemia. Twenty-nine candidate genes JJ Farrell , A Kutlar , LA Farrer , within loci previously reported to be linked to HbF level (6q22.3–q23.2, 3,1 CT Baldwin and 8q11–q12 and Xp22.2–p22.3), involved in metabolism of HU and related to MH Steinberg1 erythroid progenitor proliferation were studied in 137 sickle cell anemia patients treated with HU. Three-hundred and twenty tagging single 1Department of Medicine, Boston University nucleotide polymorphisms (SNPs) for genotyping were selected based on School of Medicine, Boston, MA, USA; HapMap data. Multiple linear regression and the nonlinear regression 2Department of Medicine, Medical College of Georgia, Augusta, GA, USA and 3Center for Random Forest method were used to investigate the association between Human Genetics, Boston University School of SNPs and the change in HbF level after 2 years of treatment with HU. Both Medicine, Boston, MA, USA methods revealed that SNPs in genes within the 6q22.3–23.2 and 8q11–q12 linkage peaks, and also the ARG2, FLT1, HAO2 and NOS1 genes were Correspondence: Dr MH Steinberg, Center of Excellence in Sickle associated with the HbF response to HU. Polymorphisms in genes regulating Cell Disease, E248, Boston Medical Center, 88 HbF expression, HU metabolism and erythroid progenitor proliferation might E. Newton Street, Boston, MA 02118, USA. modulate the patient response to HU. E-mail: [email protected] The Pharmacogenomics Journal (2007) 7, 386–394; doi:10.1038/sj.tpj.6500433; published online 13 February 2007 Keywords: SNPs; association analysis; sickle cell; fetal hemoglobin; hydroxyurea Introduction Fetal hemoglobin (HbF) inhibits the polymerization of sickle hemoglobin (HbS).1 As many of the complications of sickle cell anemia (homozygosity for HBB, glu6val), like osteonecrosis, acute chest syndrome and painful episode, are associated with the level of HbF, and, HbF is inversely associated with mortality, investigators have assiduously sought pharmacological means of increasing HbF production.2–6 Hydroxyurea (HU), a ribonucleotide reductase inhibitor, is one drug that increases HbF concentration in patients with sickle cell anemia7–10 and it is the sole FDA-approved agent for treating sickle cell anemia. Most, but not all patients respond to HU treatment with an increase in HbF, but as with the baseline HbF concentration, which varies widely among patients, the magnitude of the HbF response to HU is also variable.10–13 The regulation of HbF level might be a complex genetic trait governed by genetic elements linked to the b-globin gene-like cluster and quantitative trait loci (QTL) present on chromosomes 6, 8 and on the X-chromosome; other regulatory loci are also likely to exist and epigenetic and cellular factors could also have regulatory roles.14–27 It is possible that these and other regulatory elements also modulate the HbF response to HU. Received 6 June 2006; revised 19 September 2006; accepted 6 November 2006; published Accordingly, we hypothesized that single nucleotide polymorphisms (SNPs) online 13 February 2007 in candidate genes or QTL with putative roles in the regulation of HbF SNPs, hydroxyurea and HbF in sickle cell anemia QMaet al 387 production might modulate the HbF response to treatment SNP in a gene showing an association when the P-value for with HU. We therefore studied the association of SNPs in significance was set at 0.05. Tables 2 and 3 present the these loci with response to HU in patients who participated statistically significant results of the quantitative trait in the Multicenter Study of Hydroxyurea (MSH), a trial analysis for single SNP association with the change of HbF designed to evaluate the efficacy of this drug in sickle cell level after a 2-year treatment, expressed as percentage of anemia. total hemoglobin and as the absolute HbF level, expressed as g/dl, respectively. An analysis was also performed expressing the increment in HbF after HU treatment as F-cells. Results Seventeen SNPs were significantly associated with the change in percent HbF (Table 2). They included two in The distribution of HbF for 137 patients enrolled in this MAP3K5, five in TOX, two in NOS1, three in FLT1, two in study is shown as Figure 1. The change of HbF after HU ARG2 and two in NOS2A. The most significant association treatment did not follow a normal distribution and was observed with SNP rs2182008 (P ¼ 0.003) in FLT1 resembled a bimodal distribution with a large portion of (Fms-related tyrosine kinase 1), a vascular endothelial growth people with minor or no change (mean ¼ 0) and a small factor, which is involved in cell proliferation and differentia- portion of people with extreme change (mean 440). This tion. Twenty SNPs were significantly associated with the distribution suggested that categorizing these data, like change of absolute HbF (Table 3); a similar pattern of dividing subjects into quartiles by the HbF change, and association was observed and the most significant association comparing patients in lowest quartile group vs those was in SNP rs10483801 (P ¼ 0.0013) in ARG2 (arginase type II, patients in the highest quartile of change, might be an involved in drug metabolism of HU). Using F-cells as the alternative approach for analyzing these data. However, outcome measure gave similar results (data not shown). considering the relatively small sample size in our study, this For candidate genes with significant association in multi- approach provides very limited power for detecting genetic ple SNPs, haplotype associations were explored using associations. Haplo.stats (version 1.2.1) as given in the R library (available Three-hundred and twenty tagging SNPs in 29 candidate at http://cran.us.r-project.org).28 However, as these SNPs genes (Table 1) were examined in 137 sickle cell anemia studied here are tagging SNPs and most of them are not in patients treated with HU. We considered an SNP to have a linkage disequilibrium (LD) with each other, we did not find significant association with response to HU treatment when improved association by haplotype analysis in any genes the P-value was 0.01, unless there was more than one p (data not shown). The results of joint analysis of all the SNPs and covariates (age, sex and the a- and b-globin gene cluster haplotypes) using Random Forest analysis are shown in Figures 2 and 3. The relative importance of one independent variable (a SNP) is measured by %IncMSE (see Materials and methods for details), and the larger the value of %IncMSE, the higher importance that variable has for correct prediction of HbF response to HU. This analysis revealed that the most important variables for predicting the change of HbF level matched most of the SNPs identified by SNP association analysis. Interestingly, SNPs within ASS (arginino- succinate synthetase) and ARG1 (arginase, liver) were observed to have strong effect on the change of HbF level, which was not detected by single SNP association analysis. This suggests that these two genes might be involved in interaction with other genes to regulate the response to HU treatment. SNP rs2182008 in FLT1 showed a strong effect on response to HU treatment. This SNP was significantly associated with the change in HbF under a dominant model (P-value ¼ 0.003 for HbF in percentage and 0.002 for HbF in g/dl) and it was also a highly ranked predictor for response to HU from the Random Forest analysis (second for HbF in percentage and third for HbF in g/dl). The A allele of this SNP was associated with increased HbF level after HU treatment; there is no difference between AA and AG genotypes and the increase in HbF in subjects with these genotypes was on average 5.9 Figure 1 Distributions of HbF change (a) in percentage (%) and (b)in times higher than that in subjects with the GG genotype grams (g/dl). (Figure 4). The Pharmacogenomics Journal SNPs, hydroxyurea and HbF in sickle cell anemia QMaet al 388 Table 1 Candidate genes selected Gene Chromosomes Function Tagging SNPs Cytochrome P450, family 4, subfamily A, polypeptide 11 (CYP4A11) 1 Drug metabolism 4 Hydroxyacid oxidase 2 (long chain) (HAO2) 1 Drug metabolism 5 Kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR) 4 Cell differentiation 17 Arginase, liver (ARG1) 6 Drug metabolism 3 Phosphodiesterase 7B (PDE7B) 6 Chr6 QTL 17 Microtubule-associated protein 7 (MAP7) 6 Chr6 QTL, cell differentiation 3 Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) 6 Chr6 QTL 10 Peroxisomal biogenesis factor 7 (PEX7) 6 Chr6 QTL 6 NADPH oxidase 3 (NOX3) 6 Drug metabolism 17 Met proto-oncogene (hepatocyte growth factor receptor) (MET) 7 Cell proliferation 5 Nitric oxide synthase 3 (endothelial cell) (NOS3) 7 NO production 4 Glutathione reductase (GSR) 8 Drug metabolism 6 CCAAT/enhancer binding protein (C/EBP), delta (CEBPD) 8 Regulation of DNA 2 transcription Transcription elongation factor A (SII), 1 (TCEA1) 8 Chr8 QTL, regulation of 3 DNA transcription SRY (sex determining region Y)-box 17 (SOX17) 8 Chr8 QTL, regulation of 3 DNA transcription Thymus high mobility group box protein TOX (TOX) 8 Chr8 QTL, regulation of 57 DNA transcription Argininosuccinate synthetase (ASS) 9 NO production 24 Cytochrome P450,
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