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Sensitive Determination of Iron in Drinking Water, Mineral Water, Groundwater, and Spring Water Using Rapid Photometric Tests

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Sensitive Determination of Iron in Drinking Water, Mineral Water, Groundwater, and Spring Water Using Rapid Photometric Tests ENVIRONMENTAL Sensitive Determination of Iron in Drinking Water, Mineral Water, Groundwater, and Spring Water Using Rapid Photometric Tests Katrin Schwind, Application Scientist, Analytical Point-of-Use R&D, [email protected] Gunter Decker, Senior Global Product Manager, Analytical Point-of-Use Analytics | Photometry, [email protected] The quality of drinking water is regulated by a variety The determination of iron using the 1,10-phenanthroline of guidelines, such as the EU Council Directive 98/831,2 method according to APHA 3500-Fe B and DIN 38406-1 and WHO guideline.3 The key principles used to define enables photometric measurement down to a level of these limits consider both health hazards and sensory 0.01 mg/L, which is entirely sufficient for many samples.9 and technical reasons. Iron, for example, does not exhibit a risk for health in concentrations usually found If lower LOQs are required, the triazine method can in drinking water.2,3 However, increased concentrations be chosen. In this method, all iron ions are reduced to of iron result in the formation of iron hydroxide iron (II) ions. These react in a thioglycolate-buffered products, which can form deposits in water pipe medium containing a triazine derivative to form a systems and a brown discoloration of the water.4 red-violet complex, which is subsequently determined photometrically.10 Using a 100 mm cell and the Prove To ensure the supply of clear and colorless water, 600 UV-VIS spectrometer, LOQs for iron as low as country-specific limits have been set for drinking 0.0025 mg/L can be achieved. Due to iron removal water. The limit for iron set by the EU directive is 0.2 treatment and the naturally low iron content of most mg/L Fe,2 while the U.S. EPA specifies 0.3 mg/l Fe.5 To drinking water, preference should be given to the prevent the formation of iron deposits in water pipe more sensitive triazine method. The Spectroquant® systems, a limit of 0.02 mg/L should not be exceeded.6 Iron Test (Cat. No. 114761) has an overall measuring To ensure that the specified limits are met, drinking range of 0.0025-5.00 mg/L Fe. In the Spectroquant® water is, in many cases, subjected to a treatment photometers, the methods are pre-programmed, so no step in which the iron is precipitated. This method time-consuming calibration curve must be created. virtually eliminates any iron content, reducing the iron 6 concentration to the lower ppb range. Sample preparation and performance of the measurement with Spectroquant® Iron Test Analytical methods Samples must first be acidified with nitric acid to Highly sensitive analytical methods for trace level stabilize the iron, while carbonic acid-containing samples quantification include flame atomic absorption must also be degassed in an ultrasonic bath. A detailed spectroscopy (flame AAS, F-AAS) and optical emission description of the measurement procedure is given spectrometry with inductively coupled plasma (ICP-OES). in the application “Sensitive Measurement of Iron in Depending on the dosage volume, the measuring range Water”.11 of the F-AAS method according to DIN EN ISO 38406-32 is 0.002–0.020 mg/L Fe. The limit of quantification (LOQ) Method comparison of ICP-MS vs. Spectroquant® for the ICP-OES method according to DIN EN ISO 11885 Iron Test is 0.002 mg/L Fe.7,8 In our lab an LOQ of 0.0007 mg/L Fe is achieved by ICP-MS according to the ICH Q2 standard. The iron content of five different mineral waters was determined by Spectroquant® test kit and ICP-MS. All samples were below the LOQ of the respective Analysis of iron using analytical test kits (rapid method (0.0007 mg/L for ICP-MS, 0.0025 mg/L for photometric methods) Spectroquant® test kit. A practical alternative for swift, sensitive results The five samples were spiked with iron at three without investment in expensive instruments are different concentration levels by standard addition, and rapid photometric methods. Test kits are generally the respective recovery rates were determined by the characterized by their ease of use and speed of the photometric method. The results are shown in Table 1 procedure. The choice of the method depends on the and Figure 1. application, the measuring range, and the required accuracy. In the case of iron, two sensitive photometric methods can be chosen. The added concentrations of iron were accurately recovered. The recovery rates in the spiked samples ranged between 89% and 99% over all experiments, with an average recovery rate of 95%. (continued on next page) 7 Environmental │ Sensitive Determination of Iron in Drinking Water, Mineral Water, Groundwater, and Spring Water Using Rapid Photometric Tests Table 1. Iron content recovered after standard Figure 2: Calibration curve for the measuring range 0.0005–0.0100 mg/L Fe addition Recovered Addition concentration Mineral water [mg/L Fe] [mg/L Fe] Recovery rate Celtic 0.0050 0.0050 99% natural 0.0100 0.0089 89% 0.0250 0.0239 96% Justus Brunnen 0.0050 0.0046 91% medium 0.0100 0.0091 91% 0.0250 0.0239 96% Vitrex natural 0.0050 0.0048 95% 0.0100 0.0093 93% 0.0250 0.0238 95% Table 2: Comparison of performance Vittel natural 0.0050 0.0046 91% characteristics 0.0100 0.0095 95% Pre-programmed 0.0250 0.0241 97% method Custom calibration Volvic natural 0.0050 0.0047 93% 0.0025 – 0.5000 0.0005 – 0.0100 mg/L Fe mg/L Fe 0.0100 0.0098 98% Method standard ± 0.00328 ± 0.00023 0.0250 0.0244 98% deviation [mg/L] Method coefficient ± 1.31 ± 4.35 Figure 1: Results of the standard addition variation [%] Confidence interval ± 0.0079 ± 0.0006 (P=95 %) [mg/L] Table 3: Iron content recovered after standard addition with custom calibration Recovered Addition concentration Mineral water [mg/L Fe] [mg/L Fe] Recovery rate Celtic natural 0.0050 0.0053 106% 0.0100 0.0095 95% 0.0250 0.0255 102% Justus 0.0050 0.0049 97% Brunnen medium 0.0100 0.0097 97% 0.0250 0.0255 102% An even higher accuracy can be achieved by a custom Vitrex natural 0.0050 0.0051 102% calibration curve. Table 2 shows the performance 0.0100 0.0099 99% characteristics of the pre-programmed method for Cat. No. 114761 determined according to DIN 38402 0.0250 0.0254 102% A51 and ISO 8466-1 compared with a manually made Vittel natural 0.0050 0.0049 97% calibration curve for the measurement range 0.0005 0.0100 0.0102 102% – 0.0100 mg/l Fe using the photometric test kit. The calibration curve is shown in Figure 2. 0.0250 0.0257 103% At 4.35%, the coefficient of variation of the custom Volvic natural 0.0050 0.0050 99% calibration curve is 3.3 times higher than that of the 0.0100 0.0105 105% pre-programmed method. This is due to the fact that 0.0250 0.0261 104% at these lower concentrations, the deviations have a stronger relative effect in the custom calibration. Seen in absolute terms, the custom calibration procedure provides Since mineral waters have only low iron content, the considerably lower method errors, as shown by the experiments were also carried out using samples values of the method standard deviation and the method of groundwater and spring water, whose iron confidence interval for P=95%, which are 13 to 14 times concentrations are naturally higher due to the lack of lower than those of the pre-programmed method. any water treatment. The measurement was carried out using the pre-programmed method. Here again In the case of the standard additions, the use of such a the measurement results were verified by reference custom calibration resulted in a further enhancement of analysis using the ICP-MS method. Table 4 shows the recovery rate, which now achieved a mean value of a comparison of the results obtained with the two 101%. The individual values are between 95% and 106% methods. (see Table 3). 8 Table 4: Iron content of groundwater and spring Thermo Fisher Scientific HR-ICP mass spectrometer water – comparison of ICP-MS and Spectroquant® (method on the Element 2 device). Iron Test 114761 Featured products Concentration [mg/L Fe] Spectroquant® Description Cat. No. Groundwater and spring water ICP-MS Iron Test 114761 ® 173018 Spectroquant Prove 600 UV/VIS Spring water Bad König 0.0047 0.0041 spectrophotometer 1,8 nm spectral bandwidth ® Spring water Höchst 0.0043 0.0051 Spectroquant Iron Test 0.0025-5.00 mg/L Fe 114761 Himmelsleiter Iron Standard Solution CertiPUR® 1000 mg/l in 119781 Spring water Breitenbrunn 0.0022 < 0.0025 0.5 mol/l HNO₃ Spring water Vielbrunn 0.0017 < 0.0025 Nitric acid 65% for analysis EMSURE® ISO 100456 Spring water Rai-Breitenbach 0.0059 0.0051 Water Ultrapur 101262 Groundwater Bensheim 2.70 2.71 To read more about the Spectroquant® line for Spectrophotometric Analysis visit us at The results yielded by the Spectroquant® Iron Test SigmaAldrich.com/spectroquant are in agreement with those obtained using the ICP- MS method. Due to the very high iron content of the References Bensheim groundwater sample of 2.7 mg/L Fe, in 1. Ordinance of the DVGW (Deutscher Verein des Gas- und Wasserfach, German Technical and Scientific Association for Gas deviation from the defined procedure, a 10 mm cell was and Water), Verordnung über die Qualität von Wasser für den used. The recovery rate here was 100%. These results menschlichen Gebrauch, TrinkwV 2001, edition of 10 March 2016.
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