Posted on Authorea 13 Dec 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. curdsmins(ugadLe21) mn hm h symbiotic the them, Among environmentally numerous 2019). with Lee filled are and crypts (Jung midgut Its symbionts acquired 2017). Kikuchi and (Takeshita bifenthrin, associations insect losses, fenitrothion, economic as causing to such addition problems. In environmental Insecticides, nonnegligible to 2020). leading Li of resurgence, control yields control 2019; pest experiments, to decreased chemical and used the cage al. However, senescence, widely field et leaf been 2018). 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Data may be preliminary. h aaeeswr e sflos i ouesz 0adM istra .5 nyteenrichment the Only 0.15. = thread 2008). miss Horvath ME and and (Langfelder 50 = (3.2.2.) size R module in min package follows: WGCNA index as the set were on parameters based The performed was the WGCNA to The 2020). according (WGCNA) determined al. in analysis were et network chromosome chromosomes (Ma co-expression each to sex gene described of The used Weighted previously (MRPM) 2009). was as million al. 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No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. ee 1521,1 4401,7 0992,5 12,691 98.2% 24,255 Chr. 99.1% 20,949 97.100% Scaf. of seeds. Infestation soybean (2014). of 15,076 potential BP germination 10.1016/j.aspen.2014.04.006 Mainali, and and quality 96.6% HJ nutritional Riptortus Kim, Scaf. of SD, parasitoid encyrtid Bae, an Encyrtidae) 24,450 (Hymenoptera: Alydidae). nezarae (Hemiptera: (Hymenoptera: Ooencyrtus pedestris nezarae on Ooencyrtus insecticides on 96.7% of insecticides of effect of parasitoid effect encyrtid Ecotoxicological an Scaf. (2010). Encyrtidae) UT Lim, and 19,616 MA Alim, 98.9% References Scaf. 21,562 content 96.5% GC Genes Scaf. BUSCO (Kb) N50 Contig Scaf/Chr. Chr. No. (Kb) N50 Scaffold Level (Mb) size Total Feature study. this for help suggestion of and Z.Y.W. valuable genomes the X.W., manuscript. for provide the X.T.Y., features Z.T.S. wrote Assembly Y.X.Y., strain. H.J.H. 1 insect Table project. data. the maintained genomic whole and the the sample analyzed designed the and and collect assembled conceived the J.P.C. analyzed and H.J.H Z.T.S. J.M.L, C.X.Z., declare. CONTRIBUTIONS to AUTHOR interests competing no have authors The Project Breeding CONFLICTOFINTEREST K.C. University. Organisms and Transgenic Ningbo (2019B10004), Novel in Project Fund National Major Magna 2025 China Innovation Wong Technology the and by Science Ningbo supported (2019ZX08004-004), financially was work accession This the (female). with SRR12903498 SRA and in (male), individual) SRR12903499 deposited ACKNOWLEDGEMENTS (female (nymph), been SRR12897696 SRR12903500 have of (egg), data numbers SRR12903501 Transcriptomic accession of rese- the numbers Genome individual). with (male PRJNA674496. SRA BioProject SRR12977074 in the deposited and under been SUB8465613) have data numbers: quencing (accession genome assembled Infor- assembled NCBI The The Biotechnology the SRR12977074. in for PRJNA671796. Center numbers: BioProject National accession the the the under in with (SRA) deposited pedestris Archive been Riptortus Read have Sequence assembly (NCBI) genome mation for used reads Raw STATEMENT AVAILABILITY DATA 39841 641 2,710 13 – 97,329 29.94% 13 4 32.46% 680 13,151 947 27.1% 36 20,268 713 344 30.90% 16,537 1,150 18 1,088 32.7% 16,639 702 33.96% 802 4 15,991 1,150 13,968 340 6 1,099 181,335 1.193 pedestris Riptortus eoehsbe eoie nteNB eoe(ceso ubr:JADPXZ000000000) numbers: (accession genome NCBI the in deposited been has genome fasciatus Oncopeltus o:10.1080/09583157.2013.851170 doi: itru pedestris Riptortus halys Halyomorpha itru pedestris Riptortus 7 ertamarcescens Serratia Hmpea ldde.In: Alydidae). (Hemiptera: itru pedestris Riptortus prolixus Rhodnius saPcEntomol Asia-Pac J n te eeotr species Heteroptera other and i1gnm a endeposited been has genome Rip1 buenoi Gerris Fbiis erae the decreases (Fabricius) okEcotoxicological Book , 17 7-8.doi: 477-481. : lectularius Cimex rubrofasciata Triatoma Posted on Authorea 13 Dec 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. ug ,Km ,Km GadLe H(08.Lta n ulta ffcso ytei netcdso the on insecticides synthetic of effects sublethal and X Lethal (2018). Zhang, of DH JT, behavior Lee, Gong, feeding and HJ, and HG Kim, Huang, locomotory S, KJ, Kim, Zhang, M, AA, Jung, Hoffmann, Z, Xi, J, Y, ISME Guo, (2020). DS, XY Zhao, Hong, and XL, of dynamics Bing, Infection JF, (2018). 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(Hemiptera: ybot nielmdlsystem model ideal an symbiont: 32 6-7.di 10.1093/mol- doi: 268-274. : itru pedestris Riptortus ceso sn Nanopore using accession o clResour Ecol Mol rai marcescens erratia Genetica, optAppl Comput .pedestris R. Riptortus n in and doi: , PLoS 49 : . Posted on Authorea 13 Dec 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. oaino eei w eoe eedtrie.Ec o ersnsagene. a represents dot Each determined. were genomes two in of gene BUSCOs. synteny a missing chromosomes of M: location The BUSCOs, fragmented F: S3. and BUSCOs, complete Figure duplicated S, BUSCOs; and complete complete C, D, datasets. eukaryote BUSCOs; of and single-copy metazoan, genome. analysis arthropod, assembled insect, estimation of against analyses analyses size BUSCO genome S2. for Figure distribution depth 19-mer LC-MS/MS The pedestris by tissues S1. different Figure in proteins Identified 2. 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Riptortus Synteny The D. Posted on Authorea 13 Dec 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. al 1.Mpe ed e ilo MP)aayi fec hoooei aeand male in chromosome each of analysis (MRPM) million stages development per different reads 37 Mapped and female tissues different S14. 6 Table from data RNA-seq S13. in Table ncRNA of Summary S12. Table control. of quality annotation after functional reads S11.Gene ONT Table of in statistics factors The virulence S10. predicted Table of in analysis genes Comparative resistance S9. antibiotic Table of analysis Comparative S8. genome-assembly Table in scaffolds Bacteria-origin in S7. SSR Table of statistics in The sequences S6. repetitive Table of statistics The data. S5. Hi-C Table of statistics The assemblies. S4. contig-level Table control. of quality statistics after The reads S3. PacBio Table of database. statistics public The from S2. retrieved Table datasets protein The S1. Table ovary-speicic and of analysis (D), WGCNA testis-specific S11 Figure (C), muscle-specific illustrated. (B), were carcass-specific genes disserted (A), (E) of gut-specific analysis genes. SDS-PAGE from tissue-specific (B) terms of analysis mm. Enrichment 0.5 S10 Bar: Figure stereoscope. the under body adult tissues. fat from and ovary, disserted muscle, tissues gut, in Six genes S9. predicted Figure of analysis in KEGG genes S8. predicted Figure of in analysis genes GO predicted S7. of Figure classification function shown. KOG were databases S6. GO Figure and KEGG, KOG, in BUSCOs. Swiss-Prot, missing genes Nr, M: annotated NCBI BUSCOs, of fragmented diagram F: S5.Venn and BUSCOs, complete Figure duplicated S, BUSCOs; and complete complete genes. C, D, datasets. predicted eukaryote BUSCOs; and single-copy of metazoan, arthropod, analyses insect, against BUSCO analyses S4. Figure itru pedestris Riptortus itru pedestris Riptortus itru pedestris Riptortus itru pedestris Riptortus itru pedestri Riptortus itru pedestris Riptortus 12 itru pedestris Riptortus itru pedestris Riptortus itru pedestris Riptortus itru pedestris Riptortus ee rm3 ieetdvlpetstages. development different 37 from genes h rdce ee eesbetdt BUSCO to subjected were genes predicted The nihdKG em lf oun n GO and column) (left terms KEGG Enriched itru pedestris Riptortus . A bevto fcras testis, carcass, of Observation (A) Serratia Serratia genome. genome. genome. sp. sp. ee noae by annotated Genes genome. Posted on Authorea 13 Dec 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. 13 Posted on Authorea 13 Dec 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. 14 Posted on Authorea 13 Dec 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. 15 Posted on Authorea 13 Dec 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. 16 Posted on Authorea 13 Dec 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.160786614.42247221/v1 — This a preprint and has not been peer reviewed. Data may be preliminary. 17