Catalog # Aliquot Size

M69-31G-20 20 µg M69-31G-50 50 µg

MAST2 Recombinant human protein expressed in Sf9 cells

Catalog # M69-31G

Lot # P1699-2

Product Description Purity

Recombinant human MAST2 protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The accession number for MAST2 is BC015816. The purity of MAST2 was Gene Aliases determined to be >70% by densitometry, MAST2 approx. MW 140kDa. MAST205, MTSSK, KIAA0807

Formulation

Recombinant protein stored in 50mM Tris-HCl, pH 7.5,

150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Storage and Stability

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background

Microtubule associated serine/threonine 2 (MAST2) belongs to the AGC Ser/Thr protein kinase family. MAST2 translocations was identified in invasive breast carcinoma, which occurs during the transition to ductal carcinoma in situ (DCIS) and/or invasive carcinoma. By interacting with Phosphatase and tensin homologue (PTEN) mediated by its PDZ domain, MAST2 plays a key MAST2 Protein negative role in regulating survival pathways in neuronal Recombinant human protein expressed in Sf9 cells cells. The two interact via the PDZ domain of Catalog # MAST2. M69-31G Lot # P1699-2 References Purity >70% Concentration 0.05 µg/µl Stability 1yr at –70oC from date of shipment 1. Clay MR, et al. MAST2 and NOTCH1 translocations in breast Storage & Shipping Store product at –70oC. For optimal storage, carcinoma and associated pre-invasive lesions. Hum Pathol. aliquot target into smaller quantities after 44:2837-44, 2013 centrifugation and store at recommended 2. Delhommel F, et al. Deciphering the unconventional temperature. For most favorable performance, peptide binding to the PDZ domain of MAST2. Biochem J. avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice. 469:159-68, 2015

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.