DOCSLIB.ORG

Skin Calcium-Binding Protein Is a Parvalbumin of the Panniculus Carnosus*

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Skin Calcium-Binding Protein Is a Parvalbumin of the Panniculus Carnosus* Skin Calcium-Binding Protein Is a Parvalbumin of the Panniculus Carnosus* Pam ela Hawley-Nelson, M.S. , M artin W. Berchtold, Ph.D., H em·ik Huitfeldt, M .D., Jack Spi egel , Ph.D., and Stuart H . Yusp a, M.D. Laboraco ry of Cellular Ca rcinogenes is and T umor Promotion, Nati onal Cancer Institute (PI-1 -N , HI-I , SHY), lkthcsda, Ma rybnd; Depart ment of Cell Biology. Baylor College of Med icine (MWB) , Houston, Texas; and Department of Biology. Catholi c University of Ameri ca (PI-1 -N , JS) , Was hington, D.C. , U.S.A. Skin calcium-binding protein (SCaBP) is a ca lcium binding that the l\1,. 13,000 PV/SCaBP cross-reacting antigen was protein purified from w hole rat skin. It has a molecul ar res tricted to the hypodermal tiss ue removed by scrapin g. weig ht o f approx11nately 12,000 daltons but migrates at l\1,. Immunoflu orescent stain ing of Bouin-fixed skin sections 13,000 on sodium dodecyl sul fa te (S D S)-polyacrylamJde w ith these antisera confirmed the locali za ti on ofPV /SCaBP gels. On nitrocellulose blots of SDS-polyacrylamide gels, to the panniculus ca rnosus, a h ypodermal m uscle layer. 6 different antisera to SC aBP reacted equally wel l with N ewborn mouse skin does no t conta in this antigen. Ad­ SCaBP and parvalbumin (PV), an 11 ,500-dalton calcium­ ditional polypeptides of M ,. 10, 500 and 12,000 on SDS gel s bin ding pro tein purifi ed from rat skeletal muscle, which of extracts from the epidermis of newborn and adult rats also migrates at M ,. 13, 000 on SDS-polyacrylamide gels. and n'li ce were found to be immunoreactive with anti­ Rabbit antiserum to muscl e PV also recognized both PV SCaBP serum. T hese polypeptides were not recognized by and SCaBP, and either protein absorbed specific antibodies the PV antise rum, and the reacti vity o f anti-SCaBP fo r against either antigen fro m bo th types of antise ra. Soluble these antigens was not absorbed by purified PV o r SCaBP. protein extracts fr01n whole adult rat and m ouse skm con­ O ur results indica te that SCaBP is antigeni ca ll y indistin­ tain ed a M ,. 13,000 protein w hi ch w as recogni zed on ni­ gui shable from PV and is locali zed in the ad ult rodent trocellulose bl o ts of SDS gels by both antisera . Blots of panniculus ca rnosus, and th at antisera to SCaBP are poly­ extracts from epidermis, dermis, whole skin, and skin scraped specifi c, recognizin g epidermal proteins in addition to on the dermal side to remove hypodermal tissue revealed SCaBP/PV. ) ln lJcst Dcnnatol 86:157-162, 1986 alcium io n concentrati on has been shown to contro l T he present study was undertaken to com pa re SCaBP and PV, the differentiati o n of m o use epiderm al keratinocytes and to determ ine their tiss ue distributio n by immunohistochem­ 111 culture [1] and this regul a n o n can be altered m istry and immuno bl o t analysis, the latter technique having the ca rcinogen-treated o r m ali gnantly transformed ker­ adva ntage o f providing in fo rmation o n m o lecul ar weig ht and atinocytes [2,3]. It w as of great interest, therefo re, antigenicity simultaneously. We presen t da ta dem o nstra tin g that Cwhen a skin ca lcium-binding p rotein (SCal3 P) was identified anci PV and SCaBP are antigen ica ll y indistinguishable and that the purified fro m w ho le adult rat skin [4,5], and an tisera to this pro­ PV / SCaB P antigen is locali zed in the panniculus ca rnosus of adult tein were raised in rabbits. The locali za ti on of SC:~ BP anngen, roden t skin. Additio nal antigens of unknown nature, with M,. determined b y indirect immuno flu o rescence of frozen skin sec­ 10, 500-12,000 an sodium dodecyl sul fate (SDS)-polyacrylam ide tions, was rep orted to be in basal cells of the epidermis [6]. Its gels, are recognized b y an ti-SCaBP serum and are localized in molecular weight (1 2,000 daltons), isoe.le ctric po in t (5.0), and the epidermis of newbo rn as well as adul t rodent skin. T hese amino acid composition were similar to ano ther calcium binding antigens m ay account fo r previo us reports [6] of SCJBP in epi­ protein, p arvalbumin (PV) IS]. PV was known to be a maj o r dermis, and of regulated syn thesis of low-m olecular weight an­ compo n ent o f fast-tw itch skeletal muscle (7] and has been shown tigens in epidermal ce ll cultures r<.>. l OJ. to be present in relative abundance in adult rat skin [8], locahzed to the h y po dermal muscl e, the panniculus ca rnosus. MATERIALS AN D M ETH ODS Proteins Purified adult rat skin SCal3 J> was a gift of D r. J ana Pavlovitch, Paris, France, and was p repared as descri bed previ­ Manuscript received April 4, 1985; acce pted for publica ti on Au gust 26, o usly [4,5]. Purified adult rat muscle PV an d bovin e b rain c:t l­ 1985. This work was perfo rm ed in pa rtial ful fi ll me nt of the req uirements fo r m odulin were from Calbioch em. Adul t Sprague-Dawlcy rat and the Doctor of Philosophy deg ree of the Biology Departmcm, Ca tholic B ALB/ c m o use skin was fractionated by the procedure of Ka­ University of Ameri ca (PH-N). MWB is supported by a Swiss Nati onal wamura ct al [11] . Shaved skin was treated w ith N air and then Foundati on Fellowship for Youn g Adva nced Scicmis ts (83.0 11 .082). rem oved fro m the anim al. T he dermal side was scr:t ped vigor­ *A preliminary report of this work has been published (] Cell Bioi ously w ith a scalpel to remove the hypodermal materia l; the scraped 99: A430, 1984). skin was fl oated on 1% trypsin in sa line for 1 h at 37°C. T he Reprint reques ts to: Pa mela Hawley-Nelson, M.S., Natiomtl Ca nce r epiderm is was rem oved fro m the dcrm is by sera ping on the ep­ Institute, Building 37, Room 3A23, Bethes da, Marylan d 20892. Abbreviati ons: idermal side w ith a scalpel. N ewbo rn m o use skin was no r scraped PV : parva lbumin but was fl oated on 0. 25% try psin overnig ht at 4°C, and the ep­ SCaB P: sk in ca lcium-bin di ng protein idermis and dermis were separated w ith fo rceps. Cytosol extracts SDS: sodiu m dodecyl sul fa te were prepared fro m full-thickness skill, skin fro m w hi ch the hy- 0022-202X/86/$03.50 Copyri ght © 1986 by T he Society for In vestiga ti ve De rmatology, Inc. 157 158 HAWLEY-N ELSON ET AL THE JOUHNAL OF INVESTIGATIVE DERMATOLOGY podermal material was removed, the hypodermal material,_epi ­ A B C 0 E F G H s p PSPSPS p s p s s p c p dermis, dermis, and leg muscle by ho m ogem zmg the ti ssue 111 20 c mM T ri s, pH 7.4, w ith 1 mM EDTA and 1 mM ph.enylmethyl­ sulfonyl fluoride, freezi ng and thawing 3 times and centnfugmg at 100,000 g for 30 min, retaining the supernatant. Cell cultures - 25kd of primary newborn mouse epiderm al cells were prepared as de­ -25kd- scribed J1 2]. After 3 days of cu lture in 0.07 mM calcium medium, cell s were scraped from the dish and homogenized in the sa me - 13kd- - 13kd buffer as described for tiss ue fractions. All cytosol preparations were as sa yed for protein concentrati on by the BioRad protein assay kit. Antisera Six different unfracti o nated rabbit anti-SCal3P sera were a gift of Dr. Jana Pavlovitch, Paris, France, and were pre­ pared as previously described J4 ]. Except w here otherw ise noted, Figure 1. Parvalbumin and SCa BP cross-rea ct antigenicall y. One mi­ anti-SCaBP was from rabbit no. 3. Unfractionatcd antiserum to crogram each of purif1ed calcium-binding proteins parvalbumm (P), SC~P (5), and ca lm odulin (C) we re el ectrophoresed on reducmg SDS 15 Yo PV as well as anti-PV lgG purified from the antiserum on a PV polyac rylamide gels and transferred (blotted) to nitrocellulose. Pa11el ,4 affi nity column were prepared as described [8]. Affinity-purified was stai ned with amido bla ck. SCal31' and PV C01111 gratc at M, 13,000. anti-PV lgG was reconstituted and used at 2.2 p.g/m l_ (final di­ Pa nel s B- 1-1 we re rea cted with eq ua l dilu tions of antise ra and visualized lution). In the undiluted anti-PV serum the concentratiOn of this wi th a peroxidase rea ction as described in Mnrc ria ls and Meth ods. B =an~­ specific lgG was 1.6 m g/ml. Absorbed antisera were prepared by SCaBP, C=anti -SCal3P wh ich had been absorb ed w1t h PV D=ann­ in cubatin g 50 p.g antigen w ith 1 ml antiserum followed by cen­ SCaBP whi ch had been absorbed with SCaBP.
Load more

Recommended publications
  • Long-Lasting Muscle Thinning Induced by Infrared Irradiation Specialized with Wavelengths and Contact Cooling: a Preliminary Report
    Long-Lasting Muscle Thinning Induced by Infrared Irradiation Specialized With Wavelengths and Contact Cooling: A Preliminary Report Yohei Tanaka, MD, Kiyoshi Matsuo, MD, PhD, and Shunsuke Yuzuriha, MD, PhD Department of Plastic and Reconstructive Surgery, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan Correspondence: [email protected] Published May 28, 2010 Objective: Infrared (IR) irradiation specialized with wavelengths and contact cooling increases the amount of water in the dermis to protect the subcutaneous tissues against IR damage; thus, it is applied to smooth forehead wrinkles. However, this treatment consistently induces brow ptosis. Therefore, we investigated whether IR irradiation induces muscle thinning. Methods: Rat central back tissues were irradiated with the specialized IR device. Histological evaluation was performed on sagittal slices that included skin, panniculus carnosus, and deep muscles. Results: Significant reductions in panniculus carnosus thickness were observed between controls and irradiated tissues at postirradiation day 30 (P30), P60, P90, and P180; however, no reduction was observed in nonirradiated controls from days 0 to 180. No significant changes were observed in the trunk muscle over time. From day 0, dermal thickness was significantly reduced at P90 and P180; however, no difference was observed between P180 and nonirradiated controls at day 180. DNA degradation consistent with apoptosis was detected in the panniculus carnosus at P7 and P30. Conclusions: We found that IR irradiation induced long-lasting superficial muscle thinning, probably by a kind of apoptosis. The panniculus carnosus is equivalent to the superficial facial muscles of humans; thus, the changes observed here reflected those in the frontalis muscle that resulted in brow ptosis.
    [Show full text]
  • Anatomy of the Dog the Present Volume of Anatomy of the Dog Is Based on the 8Th Edition of the Highly Successful German Text-Atlas of Canine Anatomy
    Klaus-Dieter Budras · Patrick H. McCarthy · Wolfgang Fricke · Renate Richter Anatomy of the Dog The present volume of Anatomy of the Dog is based on the 8th edition of the highly successful German text-atlas of canine anatomy. Anatomy of the Dog – Fully illustrated with color line diagrams, including unique three-dimensional cross-sectional anatomy, together with radiographs and ultrasound scans – Includes topographic and surface anatomy – Tabular appendices of relational and functional anatomy “A region with which I was very familiar from a surgical standpoint thus became more comprehensible. […] Showing the clinical rele- vance of anatomy in such a way is a powerful tool for stimulating students’ interest. […] In addition to putting anatomical structures into clinical perspective, the text provides a brief but effective guide to dissection.” vet vet The Veterinary Record “The present book-atlas offers the students clear illustrative mate- rial and at the same time an abbreviated textbook for anatomical study and for clinical coordinated study of applied anatomy. Therefore, it provides students with an excellent working know- ledge and understanding of the anatomy of the dog. Beyond this the illustrated text will help in reviewing and in the preparation for examinations. For the practising veterinarians, the book-atlas remains a current quick source of reference for anatomical infor- mation on the dog at the preclinical, diagnostic, clinical and surgical levels.” Acta Veterinaria Hungarica with Aaron Horowitz and Rolf Berg Budras (ed.) Budras ISBN 978-3-89993-018-4 9 783899 9301 84 Fifth, revised edition Klaus-Dieter Budras · Patrick H. McCarthy · Wolfgang Fricke · Renate Richter Anatomy of the Dog The present volume of Anatomy of the Dog is based on the 8th edition of the highly successful German text-atlas of canine anatomy.
    [Show full text]
  • Skin Regeneration with All Accessory Organs Following Ablation with Irreversible Electroporation
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE RESEARCH ARTICLE J Tissue Eng Regen Med 2017. Published online in Wiley Online Library (wileyonlinelibrary.com) DOI: 10.1002/term.2374 Skin regeneration with all accessory organs following ablation with irreversible electroporation Alexander Golberg1,2*, Martin Villiger3, G. Felix Broelsch4, Kyle P. Quinn5,6, Hassan Albadawi7†, Saiqa Khan4, Michael T. Watkins7, Irene Georgakoudi5, William G. Austen Jr4, Marianna Bei1, Brett E. Bouma3,8, Martin C. Mihm Jr9 and Martin L. Yarmush1,10* 1Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA, 02114, USA 2Porter School of Environmental Studies, Tel Aviv University, Tel Aviv, Israel 3Wellman Center for Photomedicine and Department of Dermatology, Massachusetts General Hospital and Harvard Medical School, 50 Blossom Street, Boston, Massachusetts, 02114, USA 4Department of Surgery, Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA 5Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA 6Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR, 72701, USA 7Division of Vascular and Endovascular Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA 8Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, Massachusetts, 02142, USA 9Department of Dermatology, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, 02115, USA 10Department of Biomedical Engineering, Rutgers University, Piscataway, NJ, 08854, USA Abstract Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies.
    [Show full text]
  • Gen Anat-Skin
    SKIN • Cutis,integument • External covering • Skin+its appendages-- -integumentary system • Largest organ---15 to 20% body mass. LAYERS • Epidermis •Dermis Types • Thick and thin(1-5 mm thick) • Hairy and non hairy Thick skin EXAMPLES • THICK---PALMS AND SOLES BUT ANATOMICALLY THE BACK HAS THICK SKIN. REST OF BODY HAS THIN SKIN • NON HAIRY----PALMS AND SOLES,DORSAL SURFACE OF DISTAL PHALANX,GLANS PENIS,LABIA MINORA,LABIA MAJORA AND UMBLICUS FUNCTIONS • Barrier • Immunologic • Homeostasis •Sensory • Endocrine • excretory EPIDERMIS(layers) • Stratum basale or stratum germinativum • Stratum spinosum • Stratum granulosum • Stratum lucidum • Stratum corneum Type of cells in epidermis and keratinization • Keratinocytes • Melanocytes • Langerhans • Merkels cells DERMIS LAYERS---- 1.PAPILLARY • Dermal papillae • Complementary epidermal ridges or rete ridges • Dermal ridges in thick skin • Hemidesmosomes present both in dermis and epidermis RETICULAR LAYER •DENSE IRREGULAR CONNECTIVE TIISUE Sensory receptors • Free nerve endings • Ruffini end organs • Pacinian and • Meissners corpuscles Blood supply • Fasciocutaneous A • Musculocutaneous A • Direct cutaneous A APPENDAGES • Hair follicle producing hair • Sweat glands(sudoriferous) • Sebaceous glands • Nails Hair follicle • Invagination of epidermis • Parts---infundibulum, isthmus, inferior part having bulb and invagination HAIR follicle layers • Outer and inner root sheath • Types of hair vellus, terminal, club • Phases of growth— anagen, catagen and telogen Hair shaft • Cuticle •Cortex • Medulla
    [Show full text]
  • Clonal Analysis Reveals Nerve-Dependent and Independent Roles on Mammalian Hind Limb Tissue Maintenance and Regeneration
    Clonal analysis reveals nerve-dependent and independent roles on mammalian hind limb tissue maintenance and regeneration Yuval Rinkevicha,1,2, Daniel T. Montorob,1,2, Ethan Muhonenb,1, Graham G. Walmsleya,b, David Lob, Masakazu Hasegawab, Michael Januszykb, Andrew J. Connollyc, Irving L. Weissmana,2, and Michael T. Longakera,b,2 aInstitute for Stem Cell Biology and Regenerative Medicine, Department of Pathology, and Department of Developmental Biology, bHagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery, and cDepartment of Pathology, Stanford University School of Medicine, Stanford, CA 94305 Contributed by Irving L. Weissman, June 1, 2014 (sent for review January 14, 2014) The requirement and influence of the peripheral nervous system example, histopathology studies report SCI patients presenting on tissue replacement in mammalian appendages remain largely with dermal fibrosis, progressive skin thickening, and nail hy- undefined. To explore this question, we have performed genetic pertrophy on lower limbs/digits after SCI (17, 18). The severity of lineage tracing and clonal analysis of individual cells of mouse the phenotypes in these studies is progressive and directly cor- hind limb tissues devoid of nerve supply during regeneration of related with the degree of injury. the digit tip, normal maintenance, and cutaneous wound healing. To directly interrogate the peripheral nerve requirements of We show that cellular turnover, replacement, and cellular dif- mammalian hind limb tissues, we used a novel transgenic line ferentiation from presumed tissue stem/progenitor cells within that permits in vivo clonal analysis of individual cells to describe hind limb tissues remain largely intact independent of nerve and the clonal read-out of primary limb tissues in response to nerve-derived factors.
    [Show full text]
  • SKIN GRAFTS and SKIN SUBSTITUTES James F Thornton MD
    SKIN GRAFTS AND SKIN SUBSTITUTES James F Thornton MD HISTORY OF SKIN GRAFTS ANATOMY Ratner1 and Hauben and colleagues2 give excel- The character of the skin varies greatly among lent overviews of the history of skin grafting. The individuals, and within each person it varies with following highlights are excerpted from these two age, sun exposure, and area of the body. For the sources. first decade of life the skin is quite thin, but from Grafting of skin originated among the tilemaker age 10 to 35 it thickens progressively. At some caste in India approximately 3000 years ago.1 A point during the fourth decade the thickening stops common practice then was to punish a thief or and the skin once again begins to decrease in sub- adulterer by amputating the nose, and surgeons of stance. From that time until the person dies there is their day took free grafts from the gluteal area to gradual thinning of dermis, decreased skin elastic- repair the deformity. From this modest beginning, ity, and progressive loss of sebaceous gland con- skin grafting evolved into one of the basic clinical tent. tools in plastic surgery. The skin also varies greatly with body area. Skin In 1804 an Italian surgeon named Boronio suc- from the eyelid, postauricular and supraclavicular cessfully autografted a full-thickness skin graft on a areas, medial thigh, and upper extremity is thin, sheep. Sir Astley Cooper grafted a full-thickness whereas skin from the back, buttocks, palms of the piece of skin from a man’s amputated thumb onto hands and soles of the feet is much thicker.
    [Show full text]
  • The Bald Area of the Guinea-Pig
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector THE BALD AREA OF THE GIJINEAPIG* HERSCHEL S. ZACKHEIM, M.D. AND LENORE LANGS, B.S. The purpose of this report is to draw attentioncell layer. Melanocytes, as revealed by the DOPA to the presence of a hairless area behind the eartechnic, are abundant in the basal layer if the of the guinea-pig. In conversations with others,region is pigmented (Fig. 3). Directly under the it is apparent that many investigators are notepidermis is a thin layer of fine collagen fibers. aware of it. While its occurrence has been men-The bulk of the cutis is composed of coarse tioned (1), we have not found any publishedcollagen fibers similar to the cutis elsewhere. The detailed description of it. cutis of the bald region is of good thickness, but The area varies from about 1.0 to 1.5 cm. inis somewhat thinner than that of the adjacent diameter depending on the size of the animal.skin or back. Representative sections of the cutis It is symmetrically located caudal to the ear, andof the bald spot varied in thickness from 0.5 to is separated from the posterior surface of the1.0 mm., while the cutis of the adjacent skin and ear by a rim of coarse hairs. It may be completelyback ranged from 0.7 to 1.2 mm. Scattered bald or contain some scattered fine hairs (Fig. 1).striated muscle fibers were frequently found in the This region tends to be slightly larger andmid or upper cutis of the bald spot (Figs.
    [Show full text]
  • The Dermatalogy Lexicon Project (DLP)
    Rochester Institute of Technology RIT Scholar Works Presentations and other scholarship 2005 The eD rmatalogy Lexicon Project (DLP) Hintz Glen Follow this and additional works at: http://scholarworks.rit.edu/other Recommended Citation Glen, Hintz, "The eD rmatalogy Lexicon Project (DLP)" (2005). Accessed from http://scholarworks.rit.edu/other/780 This Scholarly Blog, Podcast or Website is brought to you for free and open access by RIT Scholar Works. It has been accepted for inclusion in Presentations and other scholarship by an authorized administrator of RIT Scholar Works. For more information, please contact [email protected]. index Page 1 of 1 http://www.rit.edu/~grhfad/DLP2/ 10/25/2006 index Page 1 of 1 http://www.rit.edu/~grhfad/DLP2/ 10/25/2006 DLP Viewer Page 1 of 1 Search the DLP options: 654321 Partial match 65432 Exact match 65432 by ID http://dlp.futurehealth.rochester.edu/viewer/viewer.jsp?username=tlevee&password=dlp02 10/25/2006 index Page 1 of 1 http://www.rit.edu/~grhfad/DLP2/ 10/25/2006 DLP abcess - bulla Page 1 of 1 abscess - bulla A annular asymmetric bilateral Ring shaped. 1. Pertaining to an individual lesion: Occurring or appearing on both sides abscess Unequal shape from side to side. 2. of the body, e.g., left and A localized accumulation of pus in the Pertaining to a body distribution: right arm. dermis or subcutaneous tissue. Unequal distribution of lesions on both Frequently red, warm, and tender. sides of body. Blaschko lines A skin pattern due to developmental atrophy processes usually consisting of A thinning of tissue modified by the or whorls that do not follow vascular or location, e.g., epidermal atrophy, neural structures.
    [Show full text]
  • Universidade Federal Do Ceará Faculdade De Medicina Departamento De Fisiologia E Farmacologia Programa De Pós-Graduação Em Farmacologia
    UNIVERSIDADE FEDERAL DO CEARÁ FACULDADE DE MEDICINA DEPARTAMENTO DE FISIOLOGIA E FARMACOLOGIA PROGRAMA DE PÓS-GRADUAÇÃO EM FARMACOLOGIA RICARDO DE OLIVEIRA LIMA PAPEL DA ADENOSINA E DA VIA PI3K/AKT/mTOR NO EFEITO PROTETOR DO PRÉ-CONDICIONAMENTO ISQUÊMICO REMOTO NA FORMAÇÃO DE LESÃO POR PRESSÃO EXPERIMENTAL FORTALEZA 2019 RICARDO DE OLIVEIRA LIMA PAPEL DA ADENOSINA E DA VIA PI3K/AKT/mTOR NO EFEITO PROTETOR DO PRÉ-CONDICIONAMENTO ISQUÊMICO REMOTO NA FORMAÇÃO DE LESÃO POR PRESSÃO EXPERIMENTAL Tese apresentada ao programa de Pós- graduação em Farmacologia da Universidade Federal do Ceará, como requisito parcial para obter o título de Doutor em Farmacologia. Área de concentração: Ciências Biológicas II. Orientadora: Profa. Dra. Mariana Lima Vale. FORTALEZA 2019 Dados Internacionais de Catalogação naPublicação Universidade Federal do Ceará Biblioteca Universitária Gerada automaticamente pelo módulo Catalog, mediante os dados fornecidos pelo autor L71p Lima, Ricardo de Oliveira. Papel da adenosina e da via PI3K/AKT/mTOR no efeito protetor do pré- condicionamento isquêmico remoto na formação de lesão por pressão experimental / Ricardo de Oliveira Lima. – 2019. 171 f. : il. color. Tese (doutorado) – Universidade Federal do Ceará, Faculdade de Medicina, Programa de Pós-Graduação em Farmacologia, Fortaleza, 2019. Orientação: Prof. Dr. Mariana Lima Vale. 1. Pré-condicionamento isquêmico remoto. 2. Lesão por Pressão. 3. Adenosina. I. Título. CDD 615.1 RICARDO DE OLIVEIRA LIMA PAPEL DA ADENOSINA E DA VIA PI3K/AKT/mTOR NO EFEITO PROTETOR DO PRÉ-CONDICIONAMENTO ISQUÊMICO REMOTO NA FORMAÇÃO DE LESÃO POR PRESSÃO EXPERIMENTAL Tese apresentada ao programa de Pós- graduação em Farmacologia da Universidade Federal do Ceará, como requisito parcial para obter o título de Doutor em Farmacologia.
    [Show full text]
  • Keratinocyte-Derived Follistatin Regulates Epidermal Homeostasis
    Laboratory Investigation (2009) 89, 131–141 & 2009 USCAP, Inc All rights reserved 0023-6837/09 $32.00 Keratinocyte-derived follistatin regulates epidermal homeostasis and wound repair Maria Antsiferova1, Jennifer E Klatte2,3, Eniko¨ Bodo´ 2, Ralf Paus2,4, Jose´ L Jorcano5, Martin M Matzuk6,7,8, Sabine Werner1 and Heidi Ko¨gel1 Activin is a growth and differentiation factor that controls development and repair of several tissues and organs. Transgenic mice overexpressing activin in the skin were characterized by strongly enhanced wound healing, but also by excessive scarring. In this study, we explored the consequences of targeted activation of activin in the epidermis and hair follicles by generation of mice lacking the activin antagonist follistatin in keratinocytes. We observed enhanced kerati- nocyte proliferation in the tail epidermis of these animals. After skin injury, an earlier onset of keratinocyte hyperproli- feration at the wound edge was observed in the mutant mice, resulting in an enlarged hyperproliferative epithelium. However, granulation tissue formation and scarring were not affected. These results demonstrate that selective activation of activin in the epidermis enhances reepithelialization without affecting the quality of the healed wound. Laboratory Investigation (2009) 89, 131–141; doi:10.1038/labinvest.2008.120; published online 15 December 2008 KEYWORDS: activin; dermis; epidermis; follistatin; wound healing Activins are members of the transforming growth factor-b study the role of the activin/Fst system in mature skin. In- family of growth and differentiation factors. They are dimeric terestingly, we and others previously demonstrated important proteins, consisting of two activin b subunits cross-linked by roles of activins, in particular of activin A, in tissue repair after a disulfide bridge.
    [Show full text]
  • Guideline: Assessment, Prevention and Treatment of Moisture Associated Skin Damage (MASD) in Adults & Children
    British Columbia Provincial Nursing Skin & Wound Committee Guideline: Assessment, Prevention and Treatment of Moisture-Associated Skin Damage (MASD) in Adults & Children Developed by the British Columbia Provincial Nursing Skin & Wound Committee in collaboration with Wound Clinicians from: / Title Guideline: Assessment, Prevention and Treatment of Moisture Associated Skin Damage (MASD) in Adults & Children Practice Nurses in accordance with health authority/agency policy. Level Clients with moisture-associated skin damage require an interprofessional approach to provide comprehensive, evidence-based assessment, prevention and treatment. This clinical guideline focuses solely on the role of the nurse, as one member of the interprofessional team providing client care. Background Moisture-Associated Skin Damage (MASD) is a general term for skin damage that occurs when the skin is exposed to moisture such as perspiration, urine or feces or both, wound exudate, saliva, mucous fistula, and/or stomal effluent for a prolonged period of time. MASD is differentiated from Stage 1 and Stage 2 Pressure Injuries (PI) by assessing the location of tissue damage (e.g., perianal skin damage versus a PI over a bony prominence), the wound bed characteristics (edge, colour, odour, and slough) (see Appendix A). MASD can present as mild, moderate, or severe and can occur in all client age groups: mild MASD presents as irritation and inflammation of the skin, and moderate to severe MASD presents with blistering and erosion and/or denudation of the epidermal
    [Show full text]
  • Guilherme Abbud Franco Lapin Lidocaína, Bupivacaína E Ropivacaína Sobre a Quantidade Do Peptídeo Relacionado Com Gene De
    GUILHERME ABBUD FRANCO LAPIN LIDOCAÍNA, BUPIVACAÍNA E ROPIVACAÍNA SOBRE A QUANTIDADE DO PEPTÍDEO RELACIONADO COM GENE DE CALCITONINA E SUBSTÂNCIA P NA PELE INCISADA DE RATOS. Dissertação apresentada à Universidade Federal de São Paulo, para obtenção do Título de Mestre em Ciências. SÃO PAULO 2013 GUILHERME ABBUD FRANCO LAPIN LIDOCAÍNA, BUPIVACAÍNA E ROPIVACAÍNA SOBRE A QUANTIDADE DO PEPTÍDEO RELACIONADO COM GENE DE CALCITONINA E SUBSTÂNCIA P NA PELE INCISADA DE RATOS. Dissertação apresentada à Universidade Federal de São Paulo, para obtenção do Título de Mestre em Ciências. ORIENTADOR: Prof.ª Dr.ª LYDIA MASAKO FERREIRA COORIENTADORES: Prof. Dr. BERNARDO HOCHMAN Prof. Dr. GERSON CHADI SÃO PAULO 2013 Lapin, Guilherme Abbud Franco Lidocaína, bupivacaína e ropivacaína sobre a quantidade do peptídeo relacionado com gene de calcitonina e substância p na pele incisada de ratos / Guilherme Abbud Franco Lapin. – São Paulo, 2013. xvii, 90p. Dissertação (Mestrado) – Universidade Federal de São Paulo. Programa de Pós-Graduação em Cirurgia Translacional. Título em inglês: Lidocaine, bupivacaine and ropivacaine on the amount of neuropeptides CGRP and SP in incised rat skin. 1. Inflamação Neurogênica. 2. Neuropeptídeos. 3. Substância P. 4. Peptídeo Relacionado com Gene de Calcitonina. 5. Anestésicos Locais. 6. Lidocaína. 7. Bupivacaína. 8. Pele UNIVERSIDADE FEDERAL DE SÃO PAULO PROGRAMA DE PÓS-GRADUAÇÃO EM CIRURGIA TRANSLACIONAL COORDENADOR: Prof. Dr. MIGUEL SABINO NETO iii DEDICATÓRIA À Força Divina que me apoia e me manteve firme e focado nos meus objetivos, não importasse o quão fortes fossem os reveses. iv À minha mãe, ADELE AUGUSTA ABBUD FRANCO LAPIN, uma mulher admirada pela força dos ideais, senso de justiça e generosidade, por ser minha referência de caráter, honestidade, profissionalismo e, sobretudo, por ter sempre me dado amor e apoio sob quaisquer circunstâncias.
    [Show full text]