Histology and Histopathology from Cell Biology to Tissue Engineering
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Expression and Localization of Myosin VI in Developing Mouse Spermatids
Histochem Cell Biol DOI 10.1007/s00418-017-1579-z ORIGINAL PAPER Expression and localization of myosin VI in developing mouse spermatids Przemysław Zakrzewski1 · Robert Lenartowski2 · Maria Jolanta Re˛dowicz3 · Kathryn G. Miller4 · Marta Lenartowska1 Accepted: 4 May 2017 © The Author(s) 2017. This article is an open access publication Abstract Myosin VI (MVI) is a versatile actin-based and Sertoli cell actin hoops. Since this is the frst report of motor protein that has been implicated in a variety of dif- MVI expression and localization during mouse spermio- ferent cellular processes, including endo- and exocytic genesis and MVI partners in developing sperm have not yet vesicle traffcking, Golgi morphology, and actin structure been identifed, we discuss some probable roles for MVI stabilization. A role for MVI in crucial actin-based pro- in this process. During early stages, MVI is hypothesized cesses involved in sperm maturation was demonstrated in to play a role in Golgi morphology and function as well Drosophila. Because of the prominence and importance of as in actin dynamics regulation important for attachment actin structures in mammalian spermiogenesis, we inves- of developing acrosome to the nuclear envelope. Next, tigated whether MVI was associated with actin-mediated the protein might also play anchoring roles to help gener- maturation events in mammals. Both immunofuorescence ate forces needed for spermatid head elongation. Moreo- and ultrastructural analyses using immunogold labeling ver, association of MVI with actin that accumulates in showed that MVI was strongly linked with key structures the Sertoli cell ectoplasmic specialization and other actin involved in sperm development and maturation. -
Te2, Part Iii
TERMINOLOGIA EMBRYOLOGICA Second Edition International Embryological Terminology FIPAT The Federative International Programme for Anatomical Terminology A programme of the International Federation of Associations of Anatomists (IFAA) TE2, PART III Contents Caput V: Organogenesis Chapter 5: Organogenesis (continued) Systema respiratorium Respiratory system Systema urinarium Urinary system Systemata genitalia Genital systems Coeloma Coelom Glandulae endocrinae Endocrine glands Systema cardiovasculare Cardiovascular system Systema lymphoideum Lymphoid system Bibliographic Reference Citation: FIPAT. Terminologia Embryologica. 2nd ed. FIPAT.library.dal.ca. Federative International Programme for Anatomical Terminology, February 2017 Published pending approval by the General Assembly at the next Congress of IFAA (2019) Creative Commons License: The publication of Terminologia Embryologica is under a Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) license The individual terms in this terminology are within the public domain. Statements about terms being part of this international standard terminology should use the above bibliographic reference to cite this terminology. The unaltered PDF files of this terminology may be freely copied and distributed by users. IFAA member societies are authorized to publish translations of this terminology. Authors of other works that might be considered derivative should write to the Chair of FIPAT for permission to publish a derivative work. Caput V: ORGANOGENESIS Chapter 5: ORGANOGENESIS -
Research Review Fibrocartilage
J. Anat. (1990), 171, pp. 1-15 1 Printed in Great Britain Research Review Fibrocartilage M. BENJAMIN AND E. J. EVANS Department of Anatomy, University of Wales College of Cardiff, PO Box 900, Cardif CF1 3 YF, Wales Fibrocartilage has long been a neglected tissue that is too often viewed as a poor relation of hyaline cartilage. It failed to achieve the status of a tissue with the early histologists, but it is beginning to come of age, for modem techniques are revealing some exciting secrets about fibrocartilage in knee joint menisci and intervertebral discs in particular. Yet there has never been any general review on fibrocartilage, and workers concerned with the tissue in one organ rarely consider it in another. Consequently, we lack any global picture that would encourage the spread of interest in the tissue and the effective exchange of ideas. Our review deals largely with the white fibrocartilage of standard texts and for reasons of space excludes yellow elastic cartilage. We have concentrated on fibrocartilage as a tissue rather than fibrocartilages as organs. HISTORICAL CONSIDERATIONS The most important work on cartilage in the older literature is that of Schaffer (1930). His monograph is a thorough, comparative account of cartilage and related tissues throughout the animal kingdom. The reader interested in fibrocartilage must also study Schaffer's account of chondroid tissue, for some tissues that would now be regarded as fibrocartilage were viewed by Schaffer as hyaline-cell chondroid tissue. He had a narrow vision of 'true' cartilage and called tissues where the cells were not shrunken in lacunae, 'chondroid'. -
Autologous Matrix-Induced Chondrogenesis and Generational Development of Autologous Chondrocyte Implantation
Autologous Matrix-Induced Chondrogenesis and Generational Development of Autologous Chondrocyte Implantation Hajo Thermann, MD, PhD,* Christoph Becher, MD,† Francesca Vannini, MD, PhD,‡ and Sandro Giannini, MD‡ The treatment of osteochondral defects of the talus is still controversial. Matrix-guided treatment options for covering of the defect with a scaffold have gained increasing popularity. Cellular-based autologous chondrocyte implantation (ACI) has undergone a generational development overcoming the surgical drawbacks related to the use of the periosteal flap over time. As ACI is associated with high costs and limited in availability, autologous matrix-induced chondrogenesis, a single-step procedure combining microfracturing of the subchondral bone to release bone marrow mesenchymal stem cells in combination with the coverage of an acellular matrix, has gained increasing popularity. The purposes of this report are to present the arthroscopic approach of the matrix-guided autologous matrix-induced chondrogenesis technique and generational development of ACI in the treatment of chondral and osteochon- dral defects of the talus. Oper Tech Orthop 24:210-215 C 2014 Elsevier Inc. All rights reserved. KEYWORDS cartilage, defect, ankle, talus, AMIC, ACI Introduction Cartilage repair may be obtained by cartilage replacement: (OATS, mosaicplasty) or with techniques aimed to generate a hondral and osteochondral lesions are defects of the newly formed cartilage such as microfracture or autologous Ccartilaginous surface and underlying subchondral bone of chondrocyte implantation (ACI).9-17 the talar dome. These defects are often caused by a single or Arthroscopic debridement and bone marrow stimulation multiple traumatic events, mostly inversion or eversion ankle using the microfracture technique has proven to be an 1,2 sprains in young, active patients. -
Vocabulario De Morfoloxía, Anatomía E Citoloxía Veterinaria
Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) Servizo de Normalización Lingüística Universidade de Santiago de Compostela COLECCIÓN VOCABULARIOS TEMÁTICOS N.º 4 SERVIZO DE NORMALIZACIÓN LINGÜÍSTICA Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) 2008 UNIVERSIDADE DE SANTIAGO DE COMPOSTELA VOCABULARIO de morfoloxía, anatomía e citoloxía veterinaria : (galego-español- inglés) / coordinador Xusto A. Rodríguez Río, Servizo de Normalización Lingüística ; autores Matilde Lombardero Fernández ... [et al.]. – Santiago de Compostela : Universidade de Santiago de Compostela, Servizo de Publicacións e Intercambio Científico, 2008. – 369 p. ; 21 cm. – (Vocabularios temáticos ; 4). - D.L. C 2458-2008. – ISBN 978-84-9887-018-3 1.Medicina �������������������������������������������������������������������������veterinaria-Diccionarios�������������������������������������������������. 2.Galego (Lingua)-Glosarios, vocabularios, etc. políglotas. I.Lombardero Fernández, Matilde. II.Rodríguez Rio, Xusto A. coord. III. Universidade de Santiago de Compostela. Servizo de Normalización Lingüística, coord. IV.Universidade de Santiago de Compostela. Servizo de Publicacións e Intercambio Científico, ed. V.Serie. 591.4(038)=699=60=20 Coordinador Xusto A. Rodríguez Río (Área de Terminoloxía. Servizo de Normalización Lingüística. Universidade de Santiago de Compostela) Autoras/res Matilde Lombardero Fernández (doutora en Veterinaria e profesora do Departamento de Anatomía e Produción Animal. -
An Histologie and Immunohistochemical Study
Bull Group Int Rech Sci Stomatol et Odontol - Vol 34 n° 3-4, 1991 The connective tissue cells of human dental pulp: An histologie and immunohistochemical study LYN Peifen*, FIORE-DONNO G.** and LOMBARD1 T.** * Department ofStomatology, Tbird Hospital ofBeijing Medical University, China. ** Division of Stomatology School ofDental Medicine, Faculty ofMedicine Geneva, Switzerland. SUMMARY Twenty human healthy teeth were extracted for orthondontic purposes and processed for histological, and immunohistochemical examination. Odontoblasts were pseudostratified in depth of 1-8 cells in pulpward direction showing the zone of Weil and the cell-rich zone in coronal third pulp. In the central part of pulp tissue, fibroblasts were arranged as a network. These cells strongly immunoreacted with an antibody (monoclonal and polyclonal) directed against the intermediate filament vimentin. The product reaction was specifically located in the cytoplasm. Near vessels occasional lymphocytes and mast cells were also pré¬ sent. Collagen fibers formed a plexus below the cell-rich zone in middle and coronal pulp. KEY WORDS: Pulp - Fibroblast - Man - Histology - Immunohistochemistry. RESUME Vingt dents humaines saines ont été avulsées pour des raisons orthodontiques et traitées pour être exami¬ nées du point de vue histologique et par immunohistochimie. Les odontoblastes étaient pseudostratifiés (1-8 cellules) et dans le tiers coronaire de la pulpe soit la zone de Weil une zone riche en cellules est pré¬ sente. Dans la partie centrale de la pulpe, les fibroblastes forment un réseau. Ces cellules sont fortement immuno-marquées par un anticorps (monoclonal ou polyclonal) dirigé contre la vimentine. Le marquage est spécifiquement localisé dans le cytoplasme. A proximité des vaisseaux, des mastocytes ainsi que des lymphocytes sont présents. -
Effect of Norbixin-Based Poly(Hydroxybutyrate) Membranes on the Tendon Repair Process After Tenotomy in Rats1
ACTA CIRÚRGICA BRASILEIRA ORIGINAL ARTICLE Experimental Surgery Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats1 Lízia Daniela e Silva NascimentoI , Renata Amadei NicolauII , Antônio Luiz Martins Maia FilhoIII , José Zilton Lima Verde SantosIV , Khetyma Moreira FonsecaV , Danniel Cabral Leão FerreiraVI , Rayssilane Cardoso de SousaVII , Vicente Galber Freitas VianaVIII , Luiz Fernando Meneses CarvalhoIX , José Figueredo-SilvaX I Fellow PhD degree, Postgraduate Program in Biomedical Engineering, Universidade do Vale do Paraíba (UNIVAP), Sao Jose dos Campos-SP. Assistant Professor of Kinesiology, MSc, Health Sciences Center, Physical Therapy, Universidade Estadual do Piauí (UESPI), Teresina-PI, Brazil. Conception, design, intellectual and scientific content of the study; acquisition and interpretation of data; technical procedures; manuscript preparation. II PhD, Collaborator, Postgraduate Program in Biomedical Engineering of the Research and Development Institute, UNIVAP, Sao Jose dos Campos-SP, Brazil. Conception, design, intellectual and scientific content of the study; interpretation of data; critical revision. III Associate Professor of Physiology, Health Sciences Center, UESPI, Teresina-PI, Brazil. Technical procedures. IV MSc, Assistant Professor, Center of Health Sciences, Medicine and Physical Therapy, UESPI, Teresina-PI. Brazil. Histopathological examinations. V Fellow PhD degree, Postgraduate Program in Pharmacology, Universidade Federal do Ceará (UFC), Fortaleza-CE. -
The Reproductive System
27 The Reproductive System PowerPoint® Lecture Presentations prepared by Steven Bassett Southeast Community College Lincoln, Nebraska © 2012 Pearson Education, Inc. Introduction • The reproductive system is designed to perpetuate the species • The male produces gametes called sperm cells • The female produces gametes called ova • The joining of a sperm cell and an ovum is fertilization • Fertilization results in the formation of a zygote © 2012 Pearson Education, Inc. Anatomy of the Male Reproductive System • Overview of the Male Reproductive System • Testis • Epididymis • Ductus deferens • Ejaculatory duct • Spongy urethra (penile urethra) • Seminal gland • Prostate gland • Bulbo-urethral gland © 2012 Pearson Education, Inc. Figure 27.1 The Male Reproductive System, Part I Pubic symphysis Ureter Urinary bladder Prostatic urethra Seminal gland Membranous urethra Rectum Corpus cavernosum Prostate gland Corpus spongiosum Spongy urethra Ejaculatory duct Ductus deferens Penis Bulbo-urethral gland Epididymis Anus Testis External urethral orifice Scrotum Sigmoid colon (cut) Rectum Internal urethral orifice Rectus abdominis Prostatic urethra Urinary bladder Prostate gland Pubic symphysis Bristle within ejaculatory duct Membranous urethra Penis Spongy urethra Spongy urethra within corpus spongiosum Bulbospongiosus muscle Corpus cavernosum Ductus deferens Epididymis Scrotum Testis © 2012 Pearson Education, Inc. Anatomy of the Male Reproductive System • The Testes • Testes hang inside a pouch called the scrotum, which is on the outside of the body -
Male Reproductive System
MALE REPRODUCTIVE SYSTEM DR RAJARSHI ASH M.B.B.S.(CAL); D.O.(EYE) ; M.D.-PGT(2ND YEAR) DEPARTMENT OF PHYSIOLOGY CALCUTTA NATIONAL MEDICAL COLLEGE PARTS OF MALE REPRODUCTIVE SYSTEM A. Gonads – Two ovoid testes present in scrotal sac, out side the abdominal cavity B. Accessory sex organs - epididymis, vas deferens, seminal vesicles, ejaculatory ducts, prostate gland and bulbo-urethral glands C. External genitalia – penis and scrotum ANATOMY OF MALE INTERNAL GENITALIA AND ACCESSORY SEX ORGANS SEMINIFEROUS TUBULE Two principal cell types in seminiferous tubule Sertoli cell Germ cell INTERACTION BETWEEN SERTOLI CELLS AND SPERM BLOOD- TESTIS BARRIER • Blood – testis barrier protects germ cells in seminiferous tubules from harmful elements in blood. • The blood- testis barrier prevents entry of antigenic substances from the developing germ cells into circulation. • High local concentration of androgen, inositol, glutamic acid, aspartic acid can be maintained in the lumen of seminiferous tubule without difficulty. • Blood- testis barrier maintains higher osmolality of luminal content of seminiferous tubules. FUNCTIONS OF SERTOLI CELLS 1.Germ cell development 2.Phagocytosis 3.Nourishment and growth of spermatids 4.Formation of tubular fluid 5.Support spermiation 6.FSH and testosterone sensitivity 7.Endocrine functions of sertoli cells i)Inhibin ii)Activin iii)Follistatin iv)MIS v)Estrogen 8.Sertoli cell secretes ‘Androgen binding protein’(ABP) and H-Y antigen. 9.Sertoli cell contributes formation of blood testis barrier. LEYDIG CELL • Leydig cells are present near the capillaries in the interstitial space between seminiferous tubules. • They are rich in mitochondria & endoplasmic reticulum. • Leydig cells secrete testosterone,DHEA & Androstenedione. • The activity of leydig cell is different in different phases of life. -
Morphology of the Male Reproductive Tract in the Water Scavenger Beetle Tropisternus Collaris Fabricius, 1775 (Coleoptera: Hydrophilidae)
Revista Brasileira de Entomologia 65(2):e20210012, 2021 Morphology of the male reproductive tract in the water scavenger beetle Tropisternus collaris Fabricius, 1775 (Coleoptera: Hydrophilidae) Vinícius Albano Araújo1* , Igor Luiz Araújo Munhoz2, José Eduardo Serrão3 1Universidade Federal do Rio de Janeiro, Instituto de Biodiversidade e Sustentabilidade (NUPEM), Macaé, RJ, Brasil. 2Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. 3Universidade Federal de Viçosa, Departamento de Biologia Geral, Viçosa, MG, Brasil. ARTICLE INFO ABSTRACT Article history: Members of the Hydrophilidae, one of the largest families of aquatic insects, are potential models for the Received 07 February 2021 biomonitoring of freshwater habitats and global climate change. In this study, we describe the morphology of Accepted 19 April 2021 the male reproductive tract in the water scavenger beetle Tropisternus collaris. The reproductive tract in sexually Available online 12 May 2021 mature males comprised a pair of testes, each with at least 30 follicles, vasa efferentia, vasa deferentia, seminal Associate Editor: Marcela Monné vesicles, two pairs of accessory glands (a bean-shaped pair and a tubular pair with a forked end), and an ejaculatory duct. Characters such as the number of testicular follicles and accessory glands, as well as their shape, origin, and type of secretion, differ between Coleoptera taxa and have potential to help elucidate reproductive strategies and Keywords: the evolutionary history of the group. Accessory glands Hydrophilid Polyphaga Reproductive system Introduction Coleoptera is the most diverse group of insects in the current fauna, The evolutionary history of Coleoptera diversity (Lawrence et al., with about 400,000 described species and still thousands of new species 1995; Lawrence, 2016) has been grounded in phylogenies with waiting to be discovered (Slipinski et al., 2011; Kundrata et al., 2019). -
TNF-Α–Stimulated Fibroblasts Secrete Lumican to Promote Fibrocyte Differentiation
TNF-α–stimulated fibroblasts secrete lumican to promote fibrocyte differentiation Darrell Pillinga,1, Varsha Vakilb, Nehemiah Coxa, and Richard H. Gomera,b,1 aDepartment of Biology, Texas A&M University, College Station, TX 77843; and bDepartment of Biochemistry and Cell Biology, Rice University, Houston, TX 77005 Edited by Erica Herzog, Department of Medicine, Yale University, New Haven, CT, and accepted by the Editorial Board August 12, 2015 (received for review April 15, 2015) In healing wounds and fibrotic lesions, fibroblasts and monocyte- lumican levels in the lungs, suggesting that pulmonary fibrosis derived fibroblast-like cells called fibrocytes help to form scar may be in part due to elevated lumican levels. These data suggest tissue. Although fibrocytes promote collagen production by fibro- that lumican may be one of the unknown signals from fibroblasts blasts, little is known about signaling from fibroblasts to fibrocytes. In to fibrocytes that mediates part of a fibroblast-fibrocyte feedback this report, we show that fibroblasts stimulated with the fibrocyte- loop that potentiates fibrosis. secreted inflammatory signal tumor necrosis factor-α secrete the small leucine-rich proteoglycan lumican, and that lumican, but not Results the related proteoglycan decorin, promotes human fibrocyte differ- TNF-α–Stimulated Fibroblasts Promote Fibrocyte Differentiation. To entiation. Lumican competes with the serum fibrocyte differentiation test the hypothesis that activated fibroblasts might secrete soluble inhibitor serum amyloid P, but dominates over the fibroblast-secreted factors that promote fibrocyte differentiation, we added condi- fibrocyte inhibitor Slit2. Lumican acts directly on monocytes, and un- tioned medium from human fibroblasts incubated with TNF-α like other factors that affect fibrocyte differentiation, lumican has no (FCM+TNF-α) to human peripheral blood mononuclear cells detectable effect on macrophage differentiation or polarization. -
GLOSSARY of MEDICAL and ANATOMICAL TERMS
GLOSSARY of MEDICAL and ANATOMICAL TERMS Abbreviations: • A. Arabic • abb. = abbreviation • c. circa = about • F. French • adj. adjective • G. Greek • Ge. German • cf. compare • L. Latin • dim. = diminutive • OF. Old French • ( ) plural form in brackets A-band abb. of anisotropic band G. anisos = unequal + tropos = turning; meaning having not equal properties in every direction; transverse bands in living skeletal muscle which rotate the plane of polarised light, cf. I-band. Abbé, Ernst. 1840-1905. German physicist; mathematical analysis of optics as a basis for constructing better microscopes; devised oil immersion lens; Abbé condenser. absorption L. absorbere = to suck up. acervulus L. = sand, gritty; brain sand (cf. psammoma body). acetylcholine an ester of choline found in many tissue, synapses & neuromuscular junctions, where it is a neural transmitter. acetylcholinesterase enzyme at motor end-plate responsible for rapid destruction of acetylcholine, a neurotransmitter. acidophilic adj. L. acidus = sour + G. philein = to love; affinity for an acidic dye, such as eosin staining cytoplasmic proteins. acinus (-i) L. = a juicy berry, a grape; applied to small, rounded terminal secretory units of compound exocrine glands that have a small lumen (adj. acinar). acrosome G. akron = extremity + soma = body; head of spermatozoon. actin polymer protein filament found in the intracellular cytoskeleton, particularly in the thin (I-) bands of striated muscle. adenohypophysis G. ade = an acorn + hypophyses = an undergrowth; anterior lobe of hypophysis (cf. pituitary). adenoid G. " + -oeides = in form of; in the form of a gland, glandular; the pharyngeal tonsil. adipocyte L. adeps = fat (of an animal) + G. kytos = a container; cells responsible for storage and metabolism of lipids, found in white fat and brown fat.