Viruses 2013, 5, 1655-1663; doi:10.3390/v5071655 OPEN ACCESS viruses ISSN 1999-4915 www.mdpi.com/journal/viruses Article Differentiation and Distribution of Cordyline Viruses 1–4 in Hawaiian ti Plants (Cordyline fruticosa L.) Michael Melzer 1,*, Caleb Ayin 1, Jari Sugano 2, Janice Uchida 1, Michael Kawate 1, Wayne Borth 1 and John Hu 1 1 Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, Honolulu, HI 96822, USA; E-Mails:
[email protected] (C.A);
[email protected] (J.U.);
[email protected] (M.K.);
[email protected] (W.B.);
[email protected] (J.H.) 2 Department of Plant and Environmental Protection Sciences, University of Hawaii, 45-260 Waikalua Road, Kaneohe, HI 96744, USA; E-Mail:
[email protected] * Author to whom correspondence should be addressed; E-Mail:
[email protected]; Tel.: +1-808-956-7887; Fax: +1-808-956-2832. Received: 3 May 2013; in revised form: 15 June 2013 / Accepted: 26 June 2013 / Published: 5 July 2013 Abstract: Common green ti plants (Cordyline fruticosa L.) in Hawaii can be infected by four recently characterized closteroviruses that are tentative members of the proposed genus Velarivirus. In this study, a reverse-transcription polymerase chain reaction (RT-PCR) assay developed to detect and distinguish Cordyline virus 1 (CoV-1), CoV-2, CoV-3, and CoV-4 was used to determine: (i) the distribution of these viruses in Hawaii; and (ii) if they are involved in the etiology of ti ringspot disease. One hundred and thirty-seven common green ti plants with and without ti ringspot symptoms were sampled from 43 sites on five of the Hawaiian Islands and underwent the RT-PCR assay.