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Thiol-Modified Surface Immobilization Article and Method (19) & (11) EP 2 287 610 A1 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.: 23.02.2011 Bulletin 2011/08 G01N 33/543 (2006.01) (21) Application number: 09305773.5 (22) Date of filing: 20.08.2009 (84) Designated Contracting States: • Van Zutphen, Steven AT BE BG CH CY CZ DE DK EE ES FI FR GB GR 77780 Bourron Marlotte (FR) HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR (74) Representative: Le Roux, Martine et al Designated Extension States: Cabinet Beau de Loménie AL BA RS 158, rue de l’Université 75340 Paris Cedex 07 (FR) (71) Applicant: Corning Incorporated Corning NY 14831 (US) Remarks: Claims 16 - 21 are deemed to be abandoned due to (72) Inventors: non-payment of the claims fees (Rule 45(3) EPC). • Deshayes, Sophie 77515 Faremoutiers (FR) (54) Thiol-modified surface immobilization article and method (57) A method and article to immobilize a protein, fied, surface bound polymer to immobilize the maleimide- including, for example, combining the protein and a mix- linker-modified protein on the polymer surface of the ar- ture comprised of an activated spacer compound having ticle. Also disclosed are articles having an immobilized a maleimide group in a buffer solution, to form a male- protein thereon, and to methods of using the articles hav- imide-modified protein; and contacting the maleimide- ing an immobilized protein, as defined herein. linker-modified protein and a buffer swollen, thiol-modi- EP 2 287 610 A1 Printed by Jouve, 75001 PARIS (FR) EP 2 287 610 A1 Description [0001] The entire disclosure of any publication, patent, or patent document mentioned herein is incorporated by ref- erence. 5 BACKGROUND [0002] The disclosure relates generally to an article and method for processing proteins for use in, for example, immobilization, isolation, characterization, analysis, diagnosis, or like applications. The disclosure also relates to an 10 article for use in processing proteins. SUMMARY [0003] Thedisclosure provides an article and a method for processing a target protein. Atarget protein canbe chemically 15 modified to enhance the target protein’s interaction with a thiol-modified surface of the article. The disclosure also provides an article having a thiol- modified surface for use in processing proteins, for example, immobilizing proteins and detecting ligands. BRIEF DESCRIPTION OF THE DRAWING(S) 20 [0004] In embodiments of the disclosure: [0005] Fig. 1 shows an SH-maleimide coupling reaction; [0006] Fig. 2 schematically shows the functionalization of a protein with a maleimide linker which is subsequently and specifically bound to a thiol-modified SH-surface. 25 [0007] Fig. 3 schematically illustrates a preparative sequence for a thiol surface. [0008] Fig. 4 illustrates exemplary carbonic anhydrase (CAII) immobilization results on a thiol surface. [0009] Fig. 5 illustrates furosemide binding results on immobilized carbonic anhydrase (CAII) at various concentrations of CAII. [0010] Fig. 6 illustrates exemplary carbonic anhydrase (CAII) immobilization results on a thiol surface. 30 [0011] Fig. 7 illustrates furosemide binding results on carbonic anhydrase (CAII) that has been immobilized on a thiol surface. [0012] Fig. 8 illustrates exemplary carbonic anhydrase (CAII) immobilization results on a thiol surface polymer corre- sponding to structural formula (IVa). [0013] Fig. 9 illustrates exemplary furosemide binding results on immobilized carbonic anhydrase (CAII) on a thiol 35 surface polymer corresponding to structural formula (IVa). DETAILED DESCRIPTION [0014] Various embodiments of the disclosure will be described in detail with reference to drawings, if any. Reference 40 to various embodiments does not limit the scope of the invention, which is limited only by the scope of the claims attached hereto. Additionally, any examples set forth in this specification are not limiting and merely set forth some of the many possible embodiments for the claimed invention. Definitions 45 [0015] "Assay," "assaying," or like terms refers to an analysis to determine, for example, the presence, absence, quantity, extent, kinetics, dynamics, or type of a biomolecule’s or a cell’s optical or bioimpedance response or like response or determination, upon stimulation with an exogenous stimuli, such as a ligand candidate compound. The analysis can include any of the above responses when, for example, a biomolecule interacts with the chemically modified 50 surface of the article, or when a biomolecule associated with the chemically modified surface interacts with a ligand of the biomolecule. [0016] "Attached" or like terms refers to any chemical interaction between two components or compounds. The type of chemical interaction that can be formed will vary depending upon the starting materials that are selected and reaction conditions. Examples of attachments described herein include, for example, covalent, electrostatic, ionic, hydrogen, or 55 hydrophobic bonding. "Attach," "attachment," "adhere," "adhered," "adherent," "immobilized", or like terms can generally refer to immobilizing or fixing, for example, a surface modifier substance, a surface coating polymer, a compatibilizer, a cell, a ligand candidate compound, and like entities of the disclosure, to a surface, such as by physical absorption, chemical bonding, and like processes, or combinations thereof. A biosensor surface can be modified, such as having a 2 EP 2 287 610 A1 disclosed surface coating, an anchoring or tie material, a compatibilizer (e.g., fibronectin, collagen, lamin, gelatin, poly- lysine, etc.), or like modifications, and combinations thereof, that can promote, for example, receptivity of the biosensor surface towards particular molecular or cellular entities, such as protein binding and ligand detection. [0017] "Contact" or "contacting" or like terms refer to, for example, an instance of exposure by an intimate physical 5 encounter or touching of at least one substance to another substance. [0018] "Target" or like terms refers to a cellular protein or cell-free biomolecule whose activation can mediate cell signaling or modulate cellular functions. A target can be, for example, a receptor, a phosphatase, a kinase, an enzyme, a DNA, an RNA, and like entities. A receptor can be, for example, a G protein-coupled receptor (GPCR), a receptor tyrosine kinase (RTK), a transporter, an ion channel, an integrin receptor, a sodium/proton exchanger, and like entities. 10 A kinase can be, for example, protein kinase A, protein kinase C, mitogen-activated protein (MAP) kinases, an extra- cellular signal-regulated kinases, Src, Rho kinase, focal adhesion kinase, and like entities. An enzyme can be, for example, a membrane-bound adenylyl cyclase, a soluble adenylyl cyclase, a protease, and like entities. [0019] "Screen," "screening," or like terms refers to, for example, a systematic survey of one or more compounds or drug candidates or biologicals (e.g., RNAi, antibody) to examine their pharmacological activities acting on a particular 15 target, a cell type, or a cell system. Pharmacological or biological activity is an expression describing the beneficial or adverse effects of a drug on living matter. Aspects of the disclosure are particularly useful in biosensor-based high throughput screening (HTS) applications. [0020] "Entrap," "entrapped," "entrapping," "entrapment," and like terms refer to, for example, an association of a protein with the functionalized surface of a substrate and at sufficient level for detection with, for example, optical 20 instrumentation, but the protein is not necessarily irreversibly attached to the surface, see for example Pompe (Pompe, et. al, "Functional Films of Maleic Anhydride Copolymers under Physiological Conditions," Macromol. Biosci., 2005, 5, 890-895) and commonly owned and assigned EP Application No. 09290223.8, filed March 26, 2009, entitled "Immobi- lization Method for Protein Having Low Isoelectric Point." [0021] "Immobilization," "immobilizing," "immobilize," "immobilized," and like terms refer to, for example, an association 25 of a protein with the surface of the substrate which is substantially irreversible, such as not susceptible to easy removal from the surface by, for example, mild repeated rinsing or soaking in buffer. [0022] "Protein" and like terms refers to the molecular target for entrapping or immobilization with the biosensor surface, and can include, for example, a peptide, a polypeptide, a glycoprotein, a lipoprotein, a receptor, a receptor component, an antibody, and like natural or synthetic molecules, or mixtures thereof. "Protein" can include an individual protein 30 molecule, a protein molecule in admixture, in association, in complexation, or like relations with other molecular or biological entities, including for example, whole cells, or subunits or portions thereof. [0023] "Biosensor" or like term refers to an article, that in combination with appropriate apparatus, can detect a desired analyte. A biosensor can combine a biological component with a physicochemical detector component. A biosensor can typically consist of three parts: a biological component or element (such as tissue, microorganism, pathogen, cells, cell 35 component, a receptor, and like entities, or combinations thereof), a detector element (operating in a physicochemical way such as optical, piezoelectric, electrochemical, thermometric, magnetic, or like manner), and a transducer associated with both components. In embodiments, the biosensor can convert a molecular recognition, molecular
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