Article Volume 12, Issue 3, 2022, 2826 - 2842 https://doi.org/10.33263/BRIAC123.28262842 Phytochemical Composition and Antimicrobial Properties of Burdock (Arctium lappa L.) Roots Extracts Nadezhda Petkova 1,* , Ivanka Hambarlyiska 1, Yulian Tumbarski 2 , Radka Vrancheva 3 , Miglena Raeva 1, Ivan Ivanov 1 1 Department of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria,
[email protected] (N.P.);
[email protected] (V.H.);
[email protected] (M.R.);
[email protected] (I.I.); 2 Department of Microbiology, University of Food Technologies, 26 Maritza Blvd., 4002, Plovdiv, Bulgaria;
[email protected] (Y.T.); 3 Department of Analytic and Physicochemistry, University of Food Technologies, 26 Maritza Blvd., 4002, Plovdiv, Bulgaria;
[email protected] (R.V.) ; * Correspondence:
[email protected] (N.P); Scopus Author ID 56507003400 Received: 2.06.2021; Revised: 5.07.2021; Accepted: 10.07.2021; Published: 8.08.2021 Abstract: Burdock (Arctium lappa L.) roots were used as a medicinal plant or vegetable worldwide. The research aimed to obtain different fractions by sequential extraction (hexane, chloroform, ethyl acetate water) of burdock roots and to evaluate phytochemical compounds in them. Antioxidant and antimicrobial properties of nonpolar fractions were evaluated. Ethyl acetate fraction contained the highest total phenolics, total flavonoids, and derivatives of caffeic acids. Phenolic acids (mainly chlorogenic, caffeic acid, and p-coumaric acids) were detected only in the ethyl acetate fraction, while in the hexane fraction was found only triterpenes. Due to the high polyphenol content, the ethyl acetate fraction demonstrated the highest antioxidant activity. Three fractions revealed antimicrobial activity against Salmonella sp., Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, Bacillus cereus, and Candida albicans.