Human Amelogenin (17-191, His-Tag) - Purified

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Human Amelogenin (17-191, His-Tag) - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AR50683PU-S Human Amelogenin (17-191, His-tag) - Purified Alternate names: AMELX, AMG, AMGX, Amelogenin X isoform Quantity: 20 µg Concentration: 0.25 mg/ml (determined by Bradford assay) Background: AMELX is a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in AMELX cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. Uniprot ID: Q99217 NCBI: NP_001133 GeneID: 265 Species: Human Source: E. coli Format: State: Liquid purified protein Purity: >90% by SDS - PAGE Buffer System: 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 30% glycerol, 1mM DTT Description: Recombinant human AMELX protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPLPPHP GHPGYINFSY EVLTPLKWYQ SIRPPYPSYG YEPMGGWLHH QIIPVLSQQH PPTHTLQPHH HIPVVPAQQP VIPQQPMMPV PGQHSMTPIQ HHQPNLPPPA QQPYQPQPVQ PQPHQPMQPQ PPVHPMQPLP PQPPLPPMFP MQPLPPMLPD LTLEAWPSTD KTKREEVD Molecular weight: 22 kDa (198aa), confirmed by MALDI-TOF Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: Patir A, Seymen F, et al. (2008). Caries Res. 42(5):394-400.Zhu L, Tanimoto K, et al. (2009). Cells Tissues Organs. 189(1-4):203-6. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130116 1 / 2 AR50683PU-S: Human Amelogenin (17-191, His-tag) - Purified Pictures: For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130116 2 / 2 Powered by TCPDF (www.tcpdf.org).

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Human Identification by Amelogenin Test in Libyans
American Journal of www.biomedgrid.com Biomedical Science & Research ISSN: 2642-1747 --------------------------------------------------------------------------------------------------------------------------------- Research Article Copyright@ Samir Elmrghni Human Identification by Amelogenin Test in Libyans Samir Elmrghni* and Mahmoud Kaddura Department of Forensic Medicine and Toxicology, University of Benghazi, Libya *Corresponding author: Samir Elmrghni, Faculty of Medicine, Department of Forensic Medicine and Toxicology, University of Benghazi-Libya, Benghazi, Libya. To Cite This Article: Samir Elmrghni. Human Identification by Amelogenin Test in Libyans. Am J Biomed Sci & Res. 2019 - 3(6). AJBSR. MS.ID.000737. DOI: 10.34297/AJBSR.2019.03.000737 Received: May 25, 2019 | Published: July 11, 2019 Abstract Sex typing is essential in medical diagnosis of sex-linked disease and forensic science. Gender for criminal evidence of offender is usually as reported the anomalous amelogenin results of 2 male samples (out of 238 males) represented as females (Y deletions) and another 2 samples the initial information for investigation. For individualization, identification of gender is performed in addition to the STR markers recently. We amelogenin results of the controversial samples, DNA was further used in SRY and Y-STR typing. All samples typed as males but two showed with with (X deletions) in Benghazi (Libya). The frequency in both was about 0.8%. Higher than those of the other populations reported. To confirm X chromosomes. From the results, it was highly suggested that for the controversial cases of human gender identification with amelogenin tests, amplificationKeywords: Amelogenin; of SRY gene Libyans or/and Y-STR markers will be adopted to confirm the gender [1]. Introduction gene (AMG) was precisely mapped in the p22 region on the X systems used to determine if the sample being tested is of male gene was first isolated and sequenced [2].
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Tooth Enamel and Its Dynamic Protein Matrix
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