OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AR50683PU-S Human Amelogenin (17-191, His-tag) - Purified Alternate names: AMELX, AMG, AMGX, Amelogenin X isoform Quantity: 20 µg Concentration: 0.25 mg/ml (determined by Bradford assay) Background: AMELX is a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in AMELX cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. Uniprot ID: Q99217 NCBI: NP_001133 GeneID: 265 Species: Human Source: E. coli Format: State: Liquid purified protein Purity: >90% by SDS - PAGE Buffer System: 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 30% glycerol, 1mM DTT Description: Recombinant human AMELX protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPLPPHP GHPGYINFSY EVLTPLKWYQ SIRPPYPSYG YEPMGGWLHH QIIPVLSQQH PPTHTLQPHH HIPVVPAQQP VIPQQPMMPV PGQHSMTPIQ HHQPNLPPPA QQPYQPQPVQ PQPHQPMQPQ PPVHPMQPLP PQPPLPPMFP MQPLPPMLPD LTLEAWPSTD KTKREEVD Molecular weight: 22 kDa (198aa), confirmed by MALDI-TOF Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: Patir A, Seymen F, et al. (2008). Caries Res. 42(5):394-400.Zhu L, Tanimoto K, et al. (2009). Cells Tissues Organs. 189(1-4):203-6. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130116 1 / 2 AR50683PU-S: Human Amelogenin (17-191, His-tag) - Purified Pictures: For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130116 2 / 2 Powered by TCPDF (www.tcpdf.org).