Mitochondrial Phylogeography and Subspecies of the Wide-Ranging Sub
Accepted on 18 January 2010 Ó 2010 Blackwell Verlag GmbH J Zool Syst Evol Res doi: 10.1111/j.1439-0469.2010.00565.x 1Museum of Zoology (Museum fu¨r Tierkunde), Senckenberg Dresden, Germany; 2Evolutionary Genomics Group, Department of Botany and Zoology, University of Stellenbosch, South Africa; 3Chelonian Biodiversity and Conservation, Department of Biodiversity and Conservation Biology, University of the Western Cape, Bellville, South Africa; 4c ⁄ Monte 43 Bajos, Hospitalet (Barcelona), Spain; 5Fundacio´n Andı´genA, Me´rida, Venezuela; 6Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Mitochondrial phylogeography and subspecies of the wide-ranging sub-Saharan leopard tortoise Stigmochelys pardalis (Testudines: Testudinidae) – a case study for the pitfalls of pseudogenes and GenBank sequences Uwe Fritz1,Savel R. Daniels2,Margaretha D. Hofmeyr3,Juan Gonza´lez4,Ce´sar L. Barrio-Amoro´s5,Pavel Sˇiroky´ 6, Anna.K.Hundsdo¨rfer1 and Heiko Stuckas1 Abstract The leopard tortoise (Stigmochelys pardalis) is the most widely distributed sub-Saharan tortoise species, with a range extending from the Horn of Africa all over eastern Africa to the Republic of South Africa, Namibia and southernmost Angola. Using 1938 bp of mitochondrial DNA (cyt b gene, partial ND4 gene plus adjacent tRNA genes) from a nearly range-wide sampling, we examined its phylogeographic structure and compared our findings with previously published GenBank sequences. We identified seven major clades that are largely parapatrically distributed. A few records of distinct haplotypes at the same locality or in close proximity could be the result of translocation of tortoises by man.
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