PTK2B (Human) Recombinant Protein

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PTK2B (Human) Recombinant PKB, PTK, PYK2, RAFTK Protein Gene Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in Catalog Number: P5800 calcium-induced regulation of ion channels and Regulation Status: For research use only (RUO) activation of the map kinase signaling pathway. The encoded protein may represent an important signaling Product Description: Human PTK2B (NP_775267.1, 1 intermediate between neuropeptide-activated receptors a.a. - 967 a.a.) full-length recombinant protein with GST or neurotransmitters that increase calcium flux and the tag expressed in Baculovirus infected Sf21 cells. downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine Host: Insect phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic Theoretical MW (kDa): 138 acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This Applications: Func, SDS-PAGE protein has been shown to bind CRK-associated (See our web site product page for detailed applications substrate, nephrocystin, GTPase regulator associated information) with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein Protocols: See our web site at tyrosine kinases but lacks significant sequence similarity http://www.abnova.com/support/protocols.asp or product to kinases from other subfamilies. Four transcript page for detailed protocols variants encoding two different isoforms have been Form: Liquid found for this gene. [provided by RefSeq] Preparation Method: Baculovirus infected insect cell (Sf21) expression system Purification: Glutathione sepharose chromatography Purity: 74 % by SDS-PAGE/CBB staining Activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip3000. Substrate : Blk/Lyntide. ATP: 100 µM. Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol) Storage Instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 2185 Gene Symbol: PTK2B Gene Alias: CADTK, CAKB, FADK2, FAK2, FRNK, Page 1/1 Powered by TCPDF (www.tcpdf.org).

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Supplementary Table S1. Control Capsules (CC) and Study Capsules (CR) Composition
Supplementary Table S1. Control Capsules (CC) and Study Capsules (CR) composition CC Capsules Composition mg Bioactive compound Sunflower oil 1.100 Gelatin 318,019 Glycerin 118,750 Clorophill E-140 1,923 CAPSULE TOTAL WEIGHT 1.538,692 CR Capsules Composition mg Bioactive compound Shark liver oil 20% 750 150 mg alkylglycerols alkylglycerols (Gustav Heess) Rosemary Antioxidant 11,25 mg Diterpene Phenols extract, 25 % Diterpene 45 Phenols, Type Nº 027.020 (Rosmarinus officinalis L.) Glyceril-monoestearate 30 Sunflower oil 263 Soy lecithin VEROLEC 56 12 Gelatin 318,019 Glycerin 118,750 Clorophill E-140 1,923 CAPSULE TOTAL WEIGHT 1.538,692 Supplementary Table S2. Table of the selected genes and pathways analyzed in the study Pathway Gen Gene name Inflammation, IL1B Interleukin 1, Beta Immunomodulation TNF (TNFA) Tumor Necrosis Factor MAPK1 Mitogen-Activated Protein Kinase 1 PTK2B Protein Tyrosine Kinase 2 Beta STAT3 Signal Transducer Activator Of Transcription 3 JAK1 Janus Kinase 1 JAK3 Janus Kinase 3 NFKB Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1 NLRP3 NLR Family, Pyrin Domain Containing 3 CCL2 (MCP-1) Chemokine (C-C Motif) Ligand 2 CXCR1 Chemokine (C-X-C Motif) Receptor 1 CSF2 Colony Stimulating Factor 2 (Granulocyte-Macrophage) CCL5(RANTES) Chemokine (C-C Motif) Ligand 5 CCR5 Chemokine (C-C Motif) Receptor 5 (Gene/Pseudogene) PLCG1 Phospholipase C, Gamma 1 PRKCD Protein Kinase C, Delta ADIPOQ Adiponectin, C1Q And Collagen Domain Containing BMP2 Bone Morphogenetic Protein 2 LIF Leukemia Inhibitory Factor TGFB2
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Supplementary Materials
Supplementary Suppl. Figure 1: MAPK signalling pathway of A: NCI-H2502, B: NCI-H2452, C: MSTO-211H and D: MRC-5. Suppl. Figure 2: Cell cycle pathway of A: NCI-H2502, B: NCI-H2452, C: MSTO-211H and D: MRC- 5. Suppl. Figure 3: Cancer pathways of A: NCI-H2502, B: NCI-H2452, C: MSTO-211H and D: MRC-5. Suppl. Figure 4: Phosphorylation level of A: ARAF, B: EPHA1, C: EPHA2, D: EPHA7 in all cell lines. For each cell line, phosphorylation levels are depicted before (Medium) and after cisplatin treatment (Cis). Suppl. Figure 5: Phosphorylation Level of A: KIT, B: PTPN11, C: PIK3R1, D: PTPN6 in all cell lines. For each cell line, phosphorylation levels are depicted before (Medium) and after cisplatin treatment (Cis). Suppl. Figure 6: Phosphorylation Level of A: KDR, B: EFS, C: AKT1, D: PTK2B/FAK2 in all cell lines. For each cell line, phosphorylation levels are depicted before (Medium) and after cisplatin treatment (Cis). Suppl. Figure 7: Scoreplots and volcanoplots of PTK upstream kinase analysis: A: Scoreplot of PTK- Upstream kinase analysis for NCI-H2052 cells. B: Volcanoplot of PTK-Upstream kinase analysis for NCI-H2052 cells. C: Scoreplot of PTK-Upstream kinase analysis for NCI-H2452 cells. D: Volcanoplot of PTK-Upstream kinase analysis for NCI-H2452 cells. E: Scoreplot of PTK-Upstream kinase analysis for MSTO-211H cells. F: Volcanoplot of PTK-Upstream kinase analysis for MSTO- 211H cells. G: Scoreplot of PTK-Upstream kinase analysis for MRC-5cells. H: Volcanoplot of PTK- Upstream kinase analysis for MRC-5 cells. Suppl. Figure 8: Scoreplots and volcanoplots of STK upstream kinase analysis: A: Scoreplot of STK- Upstream kinase analysis for NCI-H2052 cells.
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Blood Vitronectin Is a Major Activator of LIF and IL-6 in the Brain Through Integrin–FAK and Upar Signaling Matthew P
© 2018. Published by The Company of Biologists Ltd | Journal of Cell Science (2018) 131, jcs202580. doi:10.1242/jcs.202580 RESEARCH ARTICLE Blood vitronectin is a major activator of LIF and IL-6 in the brain through integrin–FAK and uPAR signaling Matthew P. Keasey1, Cuihong Jia1, Lylyan F. Pimentel1,2, Richard R. Sante1, Chiharu Lovins1 and Theo Hagg1,* ABSTRACT Microglia and astrocytes express the VTN receptors αvβ3 and α β We defined how blood-derived vitronectin (VTN) rapidly and potently v 5 integrin (Herrera-Molina et al., 2012; Kang et al., 2008; activates leukemia inhibitory factor (LIF) and pro-inflammatory Milner, 2009; Welser-Alves et al., 2011). Microglia and astrocytes, interleukin 6 (IL-6) in vitro and after vascular injury in the brain. as well as endothelial cells, are major producers of pro- α in vitro Treatment with VTN (but not fibrinogen, fibronectin, laminin-111 or inflammatory cytokines, such as IL-6 and TNF , and collagen-I) substantially increased LIF and IL-6 within 4 h in after traumatic or ischemic injury to the brain (Banner et al., 1997; C6-astroglioma cells, while VTN−/− mouse plasma was less effective Erta et al., 2012; Lau and Yu, 2001) or upon self-induction by IL-6 than that from wild-type mice. LIF and IL-6 were induced by (Van Wagoner and Benveniste, 1999). IL-6 is a major regulator of a intracerebral injection of recombinant human (rh)VTN in mice, but variety of inflammatory disorders and a target for therapies (Hunter induction seen upon intracerebral hemorrhage was less in VTN−/− and Jones, 2015).
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PTK2B (Human) Cell-Based ELISA Kit
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Supplementary Table 2: Infection-Sensitive Genes
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