The Bacterial Basis of Biofouling: a Case Study
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Table 1. Overview of Reactions Examined in This Study. ΔG Values Were Obtained from Thauer Et Al., 1977
Supplemental Information: Table 1. Overview of reactions examined in this study. ΔG values were obtained from Thauer et al., 1977. No. Equation ∆G°' (kJ/reaction)* Acetogenic reactions – – – + 1 Propionate + 3 H2O → Acetate + HCO3 + 3 H2 + H +76.1 Sulfate-reducing reactions – 2– – – – + 2 Propionate + 0.75 SO4 → Acetate + 0.75 HS + HCO3 + 0.25 H –37.8 2– + – 3 4 H2 + SO4 + H → HS + 4 H2O –151.9 – 2– – – 4 Acetate + SO4 → 2 HCO3 + HS –47.6 Methanogenic reactions – – + 5 4 H2 + HCO3 + H → CH4 + 3 H2O –135.6 – – 6 Acetate + H2O → CH4 + HCO3 –31.0 Syntrophic propionate conversion – – – + 1+5 Propionate + 0.75 H2O → Acetate + 0.75 CH4 + 0.25 HCO3 + 0.25 H –25.6 Complete propionate conversion by SRB – 2– – – + 2+4 Propionate + 1.75 SO4 → 1.75 HS + 3 HCO3 + 0.25 H –85.4 Complete propionate conversion by syntrophs and methanogens 1+5+6 Propionate– + 1.75 H O → 1.75 CH + 1.25 HCO – + 0.25 H+ –56.6 2 4 3 1 Table S2. Overview of all enrichment slurries fed with propionate and the total amounts of the reactants consumed and products formed during the enrichment period. The enrichment slurries consisted of sediment from either the sulfate zone (SZ), sulfate-methane transition zone (SMTZ) or methane zone (MZ) and were incubated at 25°C or 10°C, with 3 mM, 20 mM or without (-) sulfate amendments along the study. The slurries P1/P2, P3/P4, P5/P6, P7/P8 from each sediment zone are biological replicates. Slurries with * are presented in the propionate conversion graphs and used for molecular analysis. -
Bacterium That Produces Antifouling Agents
International Journal of Systematic Bacteriology (1998), 48, 1205-1 21 2 Printed in Great Britain ~- Pseudoalteromonas tunicata sp. now, a bacterium that produces antifouling agents Carola Holmstromfl Sally James,’ Brett A. Neilan,’ David C. White’ and Staffan Kjellebergl Author for correspondence : Carola Holmstrom. Tel: + 61 2 9385 260 1. Fax: + 6 1 2 9385 159 1. e-mail: c.holmstrom(cx unsw.edu.au 1 School of Microbiology A dark-green-pigmented marine bacterium, previously designated D2, which and Immunology, The produces components that are inhibitory to common marine fouling organisms University of New South Wales, Sydney 2052, has been characterized and assessed for taxonomic assignment. Based on Australia direct double-stranded sequencing of the 16s rRNA gene, D2Twas found to show the highest similarity to members of the genus 2 Center for Environmental (93%) B iotechnology, Un iversity Pseudoalteromonas. The G+C content of DZT is 42 molo/o, and it is a of Tennessee, 10515 facultatively anaerobic rod and oxidase-positive. DZT is motile by a sheathed research Drive, Suite 300, Knoxville, TN 37932, USA polar flagellum, exhibited non-fermentative metabolism and required sodium ions for growth. The strain was not capable of using citrate, fructose, sucrose, sorbitol and glycerol but it utilizes mannose and maltose and hydrolyses gelatin. The molecular evidence, together with phenotypic characteristics, showed that this bacterium which produces an antifouling agent constitutes a new species of the genus Pseudoalteromonas.The name Pseudoalteromonas tunicata is proposed for this bacterium, and the type strain is DZT (= CCUG 2 6 7 5 7T). 1 Kevwords: PseudoalttJvomonas tunicata, pigment, antifouling bacterium, marine, 16s I rRNA sequence .__ , INTRODUCTION results suggested that the genus Alteromonas should be separated into two genera. -
Antimicrobial‐Producing Pseudoalteromonas From
Received: 26 February 2018 | Revised: 11 May 2018 | Accepted: 2 June 2018 DOI: 10.1002/jobm.201800087 RESEARCH PAPER Antimicrobial-producing Pseudoalteromonas from the marine environment of Panama shows a high phylogenetic diversity and clonal structure Librada A. Atencio1,2 | Francesco Dal Grande3 | Giannina Ow Young1 | Ronnie Gavilán1,4,5 | Héctor M. Guzmán6 | Imke Schmitt3,7 | Luis C. Mejía1,6 | Marcelino Gutiérrez1 1 Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), City of Knowledge, Panama, Republic of Panama 2 Department of Biotechnology, Acharya Nagarjuna University, Guntur, India 3 Senckenberg Biodiversity and Climate Research Centre (BiK-F), Frankfurt am Main, Germany 4 National Center for Public Health, Instituto Nacional de Salud, Lima, Peru 5 Environmental Management Department, Universidad San Ignacio de Loyola, Lima, Peru 6 Smithsonian Tropical Research Institute, Ancon, Panama, Republic of Panama 7 Department of Biological Sciences, Institute of Ecology, Evolution and Diversity, Goethe Universität Frankfurt, Frankfurt, Germany Correspondence Pseudoalteromonas is a genus of marine bacteria often found in association with other Luis C. Mejía, Marcelino Gutiérrez, Centro de Biodiversidad y Descubrimiento de organisms. Although several studies have examined Pseudoalteromonas diversity and Drogas, INDICASAT-AIP, City of their antimicrobial activity, its diversity in tropical environments is largely unexplored. – Knowledge, PO 0843 01103, Panama, We investigated the diversity of Pseudoalteromonas in marine environments of Panama Republic of Panama. Email: [email protected] (L.C.M); using a multilocus phylogenetic approach. Furthermore we tested their antimicrobial [email protected] (M.G) capacity and evaluated the effect of recombination and mutation in shaping their phylogenetic relationships. -
Mono- and Multispecies Biofilms from a Crustose Coralline Alga Induce
Coral Reefs (2021) 40:381–394 https://doi.org/10.1007/s00338-021-02062-5 REPORT Mono- and multispecies biofilms from a crustose coralline alga induce settlement in the scleractinian coral Leptastrea purpurea 1 1 1 1 Lars-Erik Petersen • Mareen Moeller • Dennis Versluis • Samuel Nietzer • 1 1,2 Matthias Y. Kellermann • Peter J. Schupp Received: 18 March 2020 / Accepted: 8 January 2021 / Published online: 18 February 2021 Ó The Author(s) 2021 Abstract Microorganisms have been reported to induce was dominated by Gammaproteobacteria, Alphapro- settlement in various marine invertebrate larvae but their teobacteria, and Flavobacteria. Furthermore, we found no specificity of inductive capacities for the settlement of correlation between inductive settlement capacities and coral larvae remains poorly understood. In this study, we phylogenetic relationships. Instead, settlement behavior of isolated 56 microbial strains from the crustose coralline L. purpurea larvae was induced by specific isolated spe- alga (CCA) Hydrolithon reinboldii using five different cies. Strains #1792 (Pseudovibrio denitrificans), #1678 media either with or without additional antibiotics and/or (Acinetobacter pittii), #1633 (Pseudoalteromonas pheno- spiked CCA extract. We tested the isolates for their lica), #1772 (Marine bacterium LMG1), #1721 (Microb- potential to induce settlement behavior in larvae of the ulbifer variabilis), and #1783 (Pseudoalteromonas rubra) brooding scleractinian coral Leptastrea purpurea. From induced settlement behavior in coral larvae at mostly high -
Genomic and Phenotypic Analyses of Chitin Degradation and Secondary Metabolite Production in Pseudoalteromonas
Downloaded from orbit.dtu.dk on: Oct 06, 2021 Genomic and phenotypic analyses of chitin degradation and secondary metabolite production in Pseudoalteromonas Paulsen, Sara Skøtt Publication date: 2020 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Paulsen, S. S. (2020). Genomic and phenotypic analyses of chitin degradation and secondary metabolite production in Pseudoalteromonas. DTU Bioengineering. General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Genomic and phenotypic analyses of chitin degradation and secondary metabolite production in Pseudoalteromonas Sara Skøtt Paulsen PhD Thesis March 2020 Preface This present PhD study serves as a partial fulfillment of the requirements to obtain a PhD degree from the Technical University of Denmark (DTU). The work has has been carried out at the Department of Biotechnology and Biomedicine (DTU Bioengineering) from October 2016 to March 2020 under the supervision of Professor Lone Gram and Senior Researcher Eva Sonnenschein. -
Bacterial Diversity and Biogeography of the Cold-Water Gorgonian Primnoa Resedaeformis in Norfolk and Baltimore Canyons Christina A
617 Bacterial diversity and biogeography of the cold-water gorgonian Primnoa resedaeformis in Norfolk and Baltimore canyons Christina A. Kellogg* and Michael A. Gray U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center, St. Petersburg, Florida 33701, USA Abstract Much attention has been paid to the bacterial associates of the cold-water reef- forming scleractinian coral, Lophelia pertusa. However, many other cold-water coral species remain microbiological terra incognita, including the locally abundant, habitat-forming Atlantic octocoral Primnoa resedaeformis. During research cruises in 2012 and 2013, we collected samples from 10 individual coral colonies in each of Baltimore and Norfolk Canyons in the western Atlantic Ocean. DNA was extracted from each sample and the V4-V5 variable regions of the 16S ribosomal RNA gene were amplified and then subjected to 454 pyrosequencing. Samples were dominated by Proteobacteria followed by smaller amounts of Firmicutes, Planctomycetes, Bacteroidetes and Actinobacteria. Bacterial community sequences were found to cluster based on submarine canyon of origin. Norfolk Canyon bacterial communities had much higher representation of Gammaproteobacteria, particularly the family Moraxellaceae, compared to Baltimore Canyon communities that were dominated by Alphaproteobacteria, particularly the family Kiloniellaceae. These data provide a first Figure 2. Primnoa resedaeformis, a cold-water octocoral Figure 3. A non-metric multidimensional scaling (NMDS) plot comparing the look at the biogeographic structuring of the bacterial associates of P. resedaeformis bacterial communities of Primnoa resedaeformis from Baltimore and Norfolk likely caused by physical barriers to dispersal imposed by submarine canyons. Canyons to those of P. pacifica collected in the Gulf of Alaska (Pacific Ocean). -
The Exploration of Novel Symbiotic Bacteria That May Have Influential Roles in Sponge Life History Brittany E
University of Richmond UR Scholarship Repository Honors Theses Student Research 2008 The exploration of novel symbiotic bacteria that may have influential roles in sponge life history Brittany E. West Follow this and additional works at: https://scholarship.richmond.edu/honors-theses Part of the Biology Commons Recommended Citation West, Brittany E., "The exploration of novel symbiotic bacteria that may have influential roles in sponge life history" (2008). Honors Theses. 1082. https://scholarship.richmond.edu/honors-theses/1082 This Thesis is brought to you for free and open access by the Student Research at UR Scholarship Repository. It has been accepted for inclusion in Honors Theses by an authorized administrator of UR Scholarship Repository. For more information, please contact [email protected]. ts,,o West, B.E. lJ~s. The exploration of novel symbiotic bacteria that may have influential roles in sponge life history Brittany E. West Spring 2008 Advised by Dr. Malcolm S. Hill Department of Biology, University of Richmond This thesis has been accepted as part ofthe honors requirement in the Department of Biology. Submitted by: Approved by: Dr. Malcolm S. Hill, Honors Research Advisor 1 West, B.E. ABSTRACT: Sponges produce an impressive variety of secondary metabolites that perform a variety of ecological functions. Many marine sponges even harbor diverse carotenoid compounds, an unusual class of secondary metabolites that animals are incapable of producing. Furthermore, sponges serve as hosts to an astonishingly diverse microbial community that can occupy up to sixty percent of a sponge's biomass. Our research ultimately hopes to link microbial species to the production of secondary compounds, like carotenoids, and to assess the ecological role of such compounds and their effect on sponge life history strategy. -
A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania Huxleyi
Haverford College Haverford Scholarship Faculty Publications Biology 2016 A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania huxleyi E. L. Harvey R. W. Deering D. C. Rowley Kristen E. Whalen Haverford College, [email protected] Follow this and additional works at: https://scholarship.haverford.edu/biology_facpubs Repository Citation Harvey et al. "A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania huxleyi."Frontiers in Microbiology, 7(59):1-12, (2016). This Journal Article is brought to you for free and open access by the Biology at Haverford Scholarship. It has been accepted for inclusion in Faculty Publications by an authorized administrator of Haverford Scholarship. For more information, please contact [email protected]. ORIGINAL RESEARCH published: 03 February 2016 doi: 10.3389/fmicb.2016.00059 A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania huxleyi Elizabeth L. Harvey1*†, Robert W. Deering2, David C. Rowley2,AbrahimElGamal3, Michelle Schorn3, Bradley S. Moore3, Matthew D. Johnson4, Tracy J. Mincer5 and Kristen E. Whalen5*† 1 Department of Marine Sciences, Skidaway Institute of Oceanography, University of Georgia, Savannah, GA, USA, 2 Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI, USA, 3 Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA, USA, 4 Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA, 5 Marine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA Interactions between phytoplankton and bacteria play a central role in mediating Edited by: biogeochemical cycling and food web structure in the ocean. However, deciphering Xavier Mayali, the chemical drivers of these interspecies interactions remains challenging. -
Dynamics of the Bacterial Community Associated with Phaeodactylum Tricornutum Cultures
processes Article Dynamics of the Bacterial Community Associated with Phaeodactylum tricornutum Cultures Fiona Wanjiku Moejes 1 ID , Antonella Succurro 2,3,*,† ID , Ovidiu Popa 2,4,†, Julie Maguire 1 and Oliver Ebenhöh 2,4,* ID 1 Bantry Marine Research Station, Gearhies, Bantry P75 AX07, Co. Cork, Ireland; [email protected] (F.W.M.); [email protected] (J.M.) 2 Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany; [email protected] 3 Botanical Institute, University of Cologne, Zülpicher Strasse 47b, 50674 Cologne, Germany 4 Institute of Quantitative and Theoretical Biology, Heinrich-Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany * Correspondence: [email protected] (A.S.); [email protected] (O.E.) † These authors contributed equally to this work. Received: 17 October 2017; Accepted: 28 November 2017; Published: 7 December 2017 Abstract: The pennate diatom Phaeodactylum tricornutum is a model organism able to synthesize industrially-relevant molecules. Commercial-scale cultivation currently requires large monocultures, prone to bio-contamination. However, little is known about the identity of the invading organisms. To reduce the complexity of natural systems, we systematically investigated the microbiome of non-axenic P. tricornutum cultures from a culture collection in reproducible experiments. The results revealed a dynamic bacterial community that developed differently in “complete” and “minimal” media conditions. In complete media, we observed an accelerated “culture crash”, indicating a more stable culture in minimal media. The identification of only four bacterial families as major players within the microbiome suggests specific roles depending on environmental conditions. From our results we propose a network of putative interactions between P. -
Bioactivity, Chemical Profiling, and 16S Rrna-Based Phylogeny of Pseudoalteromonas Strains Collected on a Global Research Cruise
Downloaded from orbit.dtu.dk on: Oct 04, 2021 Bioactivity, Chemical Profiling, and 16S rRNA-Based Phylogeny of Pseudoalteromonas Strains Collected on a Global Research Cruise Vynne, Nikolaj Grønnegaard; Månsson, Maria; Nielsen, Kristian Fog; Gram, Lone Published in: Marine Biotechnology Link to article, DOI: 10.1007/s10126-011-9369-4 Publication date: 2011 Link back to DTU Orbit Citation (APA): Vynne, N. G., Månsson, M., Nielsen, K. F., & Gram, L. (2011). Bioactivity, Chemical Profiling, and 16S rRNA- Based Phylogeny of Pseudoalteromonas Strains Collected on a Global Research Cruise. Marine Biotechnology, 13(6), 1062-1073. https://doi.org/10.1007/s10126-011-9369-4 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. 1 Bioactivity, chemical profiling and 16S rRNA based phylogeny of 2 Pseudoalteromonas strains collected on a global research cruise 3 4 Nikolaj G. Vynne1*, Maria Månsson2, Kristian F. Nielsen2 and Lone Gram1 5 6 1 Technical University of Denmark, National Food Institute, Søltofts Plads, bldg. -
Adverse Effects of Immobilised Pseudoalteromonas on the Fish Pathogenic Vibrio Anguillarum: an in Vitro Study
Hindawi Publishing Corporation Journal of Marine Biology Volume 2016, Article ID 3683809, 11 pages http://dx.doi.org/10.1155/2016/3683809 Research Article Adverse Effects of Immobilised Pseudoalteromonas on the Fish Pathogenic Vibrio anguillarum: An In Vitro Study Wiebke Wesseling,1 Michael Lohmeyer,1 Sabine Wittka,1 Julia Bartels,2 Stephen Kroll,2,3 Christian Soltmann,4 Pia Kegler,5 Andreas Kunzmann,5 Sandra Neumann,6 Burkhard Ramsch,6 Beate Sellner,6 and Friedhelm Meinhardt7 1 Mikrobiologisches Labor Dr. Michael Lohmeyer GmbH, Mendelstraße 11, 48149 Munster,¨ Germany 2Advanced Ceramics, University of Bremen, Am Biologischen Garten 2, 28359 Bremen, Germany 3MAPEX Center for Materials and Processes, Bibliothekstraße 1, 28359 Bremen, Germany 4Novelpor UG, Huchtinger Heerstraße 47, 28259 Bremen, Germany 5Leibniz-Center for Tropical Marine Ecology GmbH, Fahrenheitstraße 6, 28359 Bremen, Germany 6AquaCareGmbH&Co.KG,AmWiesenbusch11,45966Gladbeck,Germany 7Institut fur¨ Molekulare Mikrobiologie und Biotechnologie, Westfalische¨ Wilhelms-Universitat¨ Munster,¨ Corrensstraße 3, 48149 Munster,¨ Germany Correspondence should be addressed to Wiebke Wesseling; [email protected] Received 7 April 2016; Revised 5 July 2016; Accepted 28 July 2016 Academic Editor: Horst Felbeck Copyright © 2016 Wiebke Wesseling et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. As a prerequisite for use in marine aquaculture, two immobilisation systems were developed by employing the probiotic bacterium Pseudoalteromonas sp. strain MLms gA3. Their impact on the survivability of the fish pathogen Vibrio anguillarum was explored. Probiotic bacteria either grown as a biofilm on ceramic tiles or embedded in alginate beads were added to sterile artificial seawater that contained the fish pathogen. -
Taxonomic Hierarchy of the Phylum Proteobacteria and Korean Indigenous Novel Proteobacteria Species
Journal of Species Research 8(2):197-214, 2019 Taxonomic hierarchy of the phylum Proteobacteria and Korean indigenous novel Proteobacteria species Chi Nam Seong1,*, Mi Sun Kim1, Joo Won Kang1 and Hee-Moon Park2 1Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea 2Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea *Correspondent: [email protected] The taxonomic hierarchy of the phylum Proteobacteria was assessed, after which the isolation and classification state of Proteobacteria species with valid names for Korean indigenous isolates were studied. The hierarchical taxonomic system of the phylum Proteobacteria began in 1809 when the genus Polyangium was first reported and has been generally adopted from 2001 based on the road map of Bergey’s Manual of Systematic Bacteriology. Until February 2018, the phylum Proteobacteria consisted of eight classes, 44 orders, 120 families, and more than 1,000 genera. Proteobacteria species isolated from various environments in Korea have been reported since 1999, and 644 species have been approved as of February 2018. In this study, all novel Proteobacteria species from Korean environments were affiliated with four classes, 25 orders, 65 families, and 261 genera. A total of 304 species belonged to the class Alphaproteobacteria, 257 species to the class Gammaproteobacteria, 82 species to the class Betaproteobacteria, and one species to the class Epsilonproteobacteria. The predominant orders were Rhodobacterales, Sphingomonadales, Burkholderiales, Lysobacterales and Alteromonadales. The most diverse and greatest number of novel Proteobacteria species were isolated from marine environments. Proteobacteria species were isolated from the whole territory of Korea, with especially large numbers from the regions of Chungnam/Daejeon, Gyeonggi/Seoul/Incheon, and Jeonnam/Gwangju.