Genomic and Phenotypic Analyses of Chitin Degradation and Secondary Metabolite Production in Pseudoalteromonas
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Bacterium That Produces Antifouling Agents
International Journal of Systematic Bacteriology (1998), 48, 1205-1 21 2 Printed in Great Britain ~- Pseudoalteromonas tunicata sp. now, a bacterium that produces antifouling agents Carola Holmstromfl Sally James,’ Brett A. Neilan,’ David C. White’ and Staffan Kjellebergl Author for correspondence : Carola Holmstrom. Tel: + 61 2 9385 260 1. Fax: + 6 1 2 9385 159 1. e-mail: c.holmstrom(cx unsw.edu.au 1 School of Microbiology A dark-green-pigmented marine bacterium, previously designated D2, which and Immunology, The produces components that are inhibitory to common marine fouling organisms University of New South Wales, Sydney 2052, has been characterized and assessed for taxonomic assignment. Based on Australia direct double-stranded sequencing of the 16s rRNA gene, D2Twas found to show the highest similarity to members of the genus 2 Center for Environmental (93%) B iotechnology, Un iversity Pseudoalteromonas. The G+C content of DZT is 42 molo/o, and it is a of Tennessee, 10515 facultatively anaerobic rod and oxidase-positive. DZT is motile by a sheathed research Drive, Suite 300, Knoxville, TN 37932, USA polar flagellum, exhibited non-fermentative metabolism and required sodium ions for growth. The strain was not capable of using citrate, fructose, sucrose, sorbitol and glycerol but it utilizes mannose and maltose and hydrolyses gelatin. The molecular evidence, together with phenotypic characteristics, showed that this bacterium which produces an antifouling agent constitutes a new species of the genus Pseudoalteromonas.The name Pseudoalteromonas tunicata is proposed for this bacterium, and the type strain is DZT (= CCUG 2 6 7 5 7T). 1 Kevwords: PseudoalttJvomonas tunicata, pigment, antifouling bacterium, marine, 16s I rRNA sequence .__ , INTRODUCTION results suggested that the genus Alteromonas should be separated into two genera. -
The Bacterial Basis of Biofouling: a Case Study
Indian Journal of Geo-Marine Sciences Vol. 43(11), November 2014, pp. 2075-2084 The Bacterial Basis of Biofouling: a Case Study Michael G. Hadfield*, Audrey Asahina, Shaun Hennings and Brian Nedved Kewalo Marine Laboratory, University of Hawaii at Manoa 41 Ahui St., Honolulu, Hawaii, 96813 USA *[E-mail: [email protected]] Received 07 March 2014; revised 14 September 2014 For billions of years bacteria have profusely colonized all parts of the oceans and formed biofilms on the benthos. Thus, from their onset, evolving marine animals adapted to the microbial world throughout their life cycles. One of the major adaptations is the use of bacterial products as signals for recruitment by larvae of many species in the seven invertebrate phyla that make up most of the biofouling community. We describe here investigations on the recruitment biology of one such species, the circum-tropical serpulid polychaete Hydroides elegans. Insights gained from in-depth studies on adults and larvae of H. elegans include: apparently constant transport of these biofouling worms on the hulls of ships maintains a globally panmictic population; larvae complete metamorphosis with little or no de novo gene transcription or translation; larvae of this tube-worm settle selectively in response to specific biofilm-dwelling bacterial species; and biofilms provide not only a cue for settlement sites, but also increase the adhesion strength of the settling worms’ tubes on a substratum. Although biofilm-bacterial species are critical to larval-settlement induction, not all biofilm elements are inductive. Because studies on chemoreception systems in the larvae failed to find evidence for the presence of common chemoreceptors, we focused on the bacterial cues themselves. -
A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania Huxleyi
Haverford College Haverford Scholarship Faculty Publications Biology 2016 A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania huxleyi E. L. Harvey R. W. Deering D. C. Rowley Kristen E. Whalen Haverford College, [email protected] Follow this and additional works at: https://scholarship.haverford.edu/biology_facpubs Repository Citation Harvey et al. "A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania huxleyi."Frontiers in Microbiology, 7(59):1-12, (2016). This Journal Article is brought to you for free and open access by the Biology at Haverford Scholarship. It has been accepted for inclusion in Faculty Publications by an authorized administrator of Haverford Scholarship. For more information, please contact [email protected]. ORIGINAL RESEARCH published: 03 February 2016 doi: 10.3389/fmicb.2016.00059 A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania huxleyi Elizabeth L. Harvey1*†, Robert W. Deering2, David C. Rowley2,AbrahimElGamal3, Michelle Schorn3, Bradley S. Moore3, Matthew D. Johnson4, Tracy J. Mincer5 and Kristen E. Whalen5*† 1 Department of Marine Sciences, Skidaway Institute of Oceanography, University of Georgia, Savannah, GA, USA, 2 Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI, USA, 3 Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA, USA, 4 Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA, 5 Marine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA Interactions between phytoplankton and bacteria play a central role in mediating Edited by: biogeochemical cycling and food web structure in the ocean. However, deciphering Xavier Mayali, the chemical drivers of these interspecies interactions remains challenging. -
Adverse Effects of Immobilised Pseudoalteromonas on the Fish Pathogenic Vibrio Anguillarum: an in Vitro Study
Hindawi Publishing Corporation Journal of Marine Biology Volume 2016, Article ID 3683809, 11 pages http://dx.doi.org/10.1155/2016/3683809 Research Article Adverse Effects of Immobilised Pseudoalteromonas on the Fish Pathogenic Vibrio anguillarum: An In Vitro Study Wiebke Wesseling,1 Michael Lohmeyer,1 Sabine Wittka,1 Julia Bartels,2 Stephen Kroll,2,3 Christian Soltmann,4 Pia Kegler,5 Andreas Kunzmann,5 Sandra Neumann,6 Burkhard Ramsch,6 Beate Sellner,6 and Friedhelm Meinhardt7 1 Mikrobiologisches Labor Dr. Michael Lohmeyer GmbH, Mendelstraße 11, 48149 Munster,¨ Germany 2Advanced Ceramics, University of Bremen, Am Biologischen Garten 2, 28359 Bremen, Germany 3MAPEX Center for Materials and Processes, Bibliothekstraße 1, 28359 Bremen, Germany 4Novelpor UG, Huchtinger Heerstraße 47, 28259 Bremen, Germany 5Leibniz-Center for Tropical Marine Ecology GmbH, Fahrenheitstraße 6, 28359 Bremen, Germany 6AquaCareGmbH&Co.KG,AmWiesenbusch11,45966Gladbeck,Germany 7Institut fur¨ Molekulare Mikrobiologie und Biotechnologie, Westfalische¨ Wilhelms-Universitat¨ Munster,¨ Corrensstraße 3, 48149 Munster,¨ Germany Correspondence should be addressed to Wiebke Wesseling; [email protected] Received 7 April 2016; Revised 5 July 2016; Accepted 28 July 2016 Academic Editor: Horst Felbeck Copyright © 2016 Wiebke Wesseling et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. As a prerequisite for use in marine aquaculture, two immobilisation systems were developed by employing the probiotic bacterium Pseudoalteromonas sp. strain MLms gA3. Their impact on the survivability of the fish pathogen Vibrio anguillarum was explored. Probiotic bacteria either grown as a biofilm on ceramic tiles or embedded in alginate beads were added to sterile artificial seawater that contained the fish pathogen. -
Spotlight on Antimicrobial Metabolites from the Marine Bacteria Pseudoalteromonas: Chemodiversity and Ecological Significance
marine drugs Review Spotlight on Antimicrobial Metabolites from the Marine Bacteria Pseudoalteromonas: Chemodiversity and Ecological Significance Clément Offret, Florie Desriac †, Patrick Le Chevalier, Jérôme Mounier, Camille Jégou and Yannick Fleury * Laboratoire Universitaire de Biodiversité et d’Ecologie Microbienne LUBEM EA3882, Université de Brest, Technopole Brest-Iroise, 29280 Plouzané, France; [email protected] (C.O.); fl[email protected] (F.D.); [email protected] (P.L.C.); [email protected] (J.M.); [email protected] (C.J.) * Correspondence: yannick.fl[email protected]; Tel.: +33-298-641-935 † Present address: University of Lille, INRA, ISA; University of Artois; University of Littoral Côte d’Opale; Institute of Charles Viollette, EA 7394 Lille, France. Academic Editor: Paola Laurienzo Received: 30 May 2016; Accepted: 29 June 2016; Published: 8 July 2016 Abstract: This review is dedicated to the antimicrobial metabolite-producing Pseudoalteromonas strains. The genus Pseudoalteromonas hosts 41 species, among which 16 are antimicrobial metabolite producers. To date, a total of 69 antimicrobial compounds belonging to 18 different families have been documented. They are classified into alkaloids, polyketides, and peptides. Finally as Pseudoalteromonas strains are frequently associated with macroorganisms, we can discuss the ecological significance of antimicrobial Pseudoalteromonas as part of the resident microbiota. Keywords: Pseudoalteromonas; antimicrobial metabolites; alkaloid; polyketide; non ribosomal peptide; genome mining; marine host-associated microbiota; probiotic 1. Introduction Last October, we celebrated the 20th anniversary of the genus Pseudoalteromonas having been split from Alteromonas [1]. The genus Pseudoalteromonas includes Gram-negative, heterotrophic, and aerobic bacteria with a polar flagellum and has a GC content comprised between 38% and 50% [2]. -
What We Can Learn from Sushi: a Review on Seaweed–Bacterial Associations Joke Hollants1,2, Frederik Leliaert2, Olivier De Clerck2 & Anne Willems1
MINIREVIEW What we can learn from sushi: a review on seaweed–bacterial associations Joke Hollants1,2, Frederik Leliaert2, Olivier De Clerck2 & Anne Willems1 1Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium; and 2Phycology Research Group, Department of Biology, Ghent University, Ghent, Belgium Correspondence: Joke Hollants, Ghent Abstract University, Department of Biochemistry and Microbiology (WE10), Laboratory of Many eukaryotes are closely associated with bacteria which enable them to Microbiology, K.L. Ledeganckstraat 35, expand their physiological capacities. Associations between algae (photosyn- B-9000 Ghent, Belgium. thetic eukaryotes) and bacteria have been described for over a hundred years. Tel.: +32 9 264 5140; fax: +32 9 264 5092; A wide range of beneficial and detrimental interactions exists between macroal- e-mail: [email protected] gae (seaweeds) and epi- and endosymbiotic bacteria that reside either on the surface or within the algal cells. While it has been shown that these chemically Received 6 April 2012; revised 27 June 2012; accepted 3 July 2012. mediated interactions are based on the exchange of nutrients, minerals, and secondary metabolites, the diversity and specificity of macroalgal–bacterial rela- DOI: 10.1111/j.1574-6941.2012.01446.x tionships have not been thoroughly investigated. Some of these alliances have been found to be algal or bacterial species-specific, whereas others are wide- Editor: Lily Young spread among different symbiotic partners. Reviewing 161 macroalgal–bacterial studies from the last 55 years, a definite bacterial core community, consisting Keywords of Gammaproteobacteria, CFB group, Alphaproteobacteria, Firmicutes, and bacteria; diversity; interaction; macroalgae; Actinobacteria species, seems to exist which is specifically (functionally) symbiosis. -
Identification and Classification of Marine Bacteria Associated with Poly(Ethylene Terephthalate) Degradation
Identification and classification of marine bacteria associated with poly(ethylene terephthalate) degradation Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy by Hooi Jun Ng Department of Chemistry and Biotechnology School of Science Faculty of Science, Engineering and Technology Swinburne University of Technology November 2014 Abstract Poly(ethylene terephthalate) (PET) is manmade synthetic polymer that has been widely used over the past few decades due to its low manufacturing cost, together with desirable properties. The high production and usage of PET, together with the inappropriate handling of resultant wastes are becoming a major global environmental issue, especially in the marine environment due to the fact that PET is fairly stable and not easily degraded in the environment. The waste handling methods that are currently available, such as burying, incineration and recycling, have their own drawbacks and limitations. Biodegradation represents an environmentally friendly, cost effective and potentially more efficient method for the management of PET, as can be concluded historical instances where microorganisms have been shown to be capable remediating and biodegrading environmental pollutants. The potential with which microorganisms could adapt to mineralize PET has previously been reported, however, none of the studies have identified the potential of marine bacteria to biodegrade of PET. In this project, a collection of marine bacteria belonging to two phylotypes, Alpha- and Gammaproteobacteria, which might have the potential to biodegrade PET, have been investigated to examine their ability to degrade PET. One strain, affiliated to the genus Marinobacter, and designated as A3d10T, was identified to have the ability to hydrolyze bis(benzoyloxyethyl) terephthalate, a trimer of PET. -
BIOSYNTHESIS of PRODIGININE and TAMBJAMINE NATURAL PRODUCTS in PSEUDOALTEROMONAS SP. by Katherine J. Picott a Thesis Submitted T
BIOSYNTHESIS OF PRODIGININE AND TAMBJAMINE NATURAL PRODUCTS IN PSEUDOALTEROMONAS SP. by Katherine J. Picott A thesis submitted to the Department of Biomedical and Molecular Sciences in conformity with the requirements for the degree of Master of Science Queen’s University Kingston, Ontario, Canada October, 2018 Copyright © Katherine J. Picott, 2018 ‘The more that you read, the more things you will know. The more that you learn, the more places you’ll go” – Dr. Suess ii Abstract Prodiginines and tambjamines are two classes of pigmented natural products that display structural variety and hold many useful bioactivities, making them a desirable target for pharmaceutical agents1. Enzymes PigC and TamQ are responsible for the final step in prodiginine and tambjamine biosynthesis respectively, involving the coupling of a product specific intermediate to the shared intermediate 4-methoxy-2,2’-bipyrrole-5-carbaldehyde2. Previous investigations have focused solely on PigC and have not successfully purified the enzyme, inciting the use of crude cell lysate to test enzyme activity3,4. The objectives of this research were to directly compare the kinetic characteristics and substrate selectivity of PigC and TamQ. PigC was cloned from Pseudoalteromonas rubra, a known producer of several prodiginine analogues5,6. TamQ was cloned from Pseudoalteromonas citrea, which is predicted to produce tambjamine YP1 based on genomic mining. PigC and TamQ were successfully heterologously expressed and purified, and enzymatic activities of both PigC and TamQ were reconstituted with their native and pseudo-native substrates. The true kinetic parameters of two substrates for PigC were determined, allowing for prediction of the reaction sequence and a proposed Ter Bi Ping Pong enzyme system. -
Assessing the Potential Bacterial Origin of the Chemical Diversity in Calcareous Sponges
36 Journal of Marine Science and Technology, Vol. 22, No. 1, pp. 36-49 (2014) DOI: 10.6119/JMST-013-0718-2 ASSESSING THE POTENTIAL BACTERIAL ORIGIN OF THE CHEMICAL DIVERSITY IN CALCAREOUS SPONGES Elodie Quévrain1, Mélanie Roué1, Isabelle Domart-Coulon1, 2, 1 and Marie-Lise Bourguet-Kondracki Key words: calcareous sponges, cultivable bacteria, antimicrobial with a few Firmicutes, Actinobacteria and Bacteroidetes. A activity, bacterial antagonisms, chemical mediation of range of biologically active compounds were purified from interactions. crude extracts of sponge or from culture broths of bacterial isolates. Ecological implications for the host sponge are being discussed based on localization and abundance of the ABSTRACT producing bacteria in situ in the sponge tissue and on the The chemodiversity and cultivable bacterial diversity of co-detection of the chemical fingerprint of the bacterial me- temperate calcareous sponges were investigated in a time series tabolites in the host extracts. Some bacterial compounds were of collection of two sponges, Leuconia johnstoni (Baerida, shown to have a role as chemical mediators of interactions Calcaronea) collected from the northeastern Atlantic Ocean within the sponge-associated bacterial compartment. and Clathrina clathrus (Clathrinida, Calcinea) collected from the northwestern Mediterranean Sea, using combined chemi- I. INTRODUCTION cal and microbiological approaches. Bacteria were visualized in tissue sections of these sponges The oceans which cover more than 70% of the earth’s sur- with Gram staining and in situ hybridization. The sponge face and contain more than 500,000 described species of crude extracts revealed annually persistent biological activi- plants and animals are a rich source of biodiversity, with rep- ties against reference human pathogen strains: L. -
Antimicrobially Active Microorganisms Associated with Marine Bryozoans
Antimicrobially active microorganisms associated with marine bryozoans Wirkstoffproduzierende Mikroorganismen assoziiert mit marinen Bryozoen Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakultät der Christian-Albrechts-Universität zu Kiel vorgelegt von Herwig Heindl Kiel, 2011 Referent/in: Prof. Dr. Johannes F. Imhoff Koreferent/in: Prof. Dr. Peter Schönheit Tag der mündlichen Prüfung: 13.05.2011 Zum Druck genehmigt: Kiel, 13.05.2011 gez. Prof. Dr. Lutz Kipp, Dekan II In Erinnerung an meine Oma “I love deadlines. I like the whooshing sound they make as they fly by.” Douglas Adams (1952 – 2001) III Table of contents Summary .................................................................................................................................... 1 Zusammenfassung...................................................................................................................... 2 General introduction................................................................................................................... 4 Bryozoans............................................................................................................................... 4 Bryozoan-natural products ..................................................................................................... 8 Bryozoan-microbe associations............................................................................................ 11 The role of natural products in drug discovery - a focus on anti-infectives........................ -
Pseudoalteromonas Rhizosphaerae Sp. Nov., a Novel Plant Growth- Promoting Bacterium with Potential Use in Phytoremediation
TAXONOMIC DESCRIPTION Navarro- Torre et al., Int. J. Syst. Evol. Microbiol. 2020;70:3287–3294 DOI 10.1099/ijsem.0.004167 Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth- promoting bacterium with potential use in phytoremediation Salvadora Navarro- Torre1, Lorena Carro2, Ignacio D. Rodríguez- Llorente1, Eloísa Pajuelo1, Miguel Ángel Caviedes1, José Mariano Igual3, Hans- Peter Klenk4 and Maria del Carmen Montero- Calasanz4,* Abstract Strain RA15T was isolated from the rhizosphere of the halophyte plant Arthrocnemum macrostachyum growing in the Odiel marshes (Huelva, Spain). RA15T cells were Gram stain- negative, non- spore- forming, aerobic rods and formed cream- coloured, opaque, mucoid, viscous, convex, irregular colonies with an undulate margin. Optimal growth conditions were observed on tryptic soy agar (TSA) plates supplemented with 2.5 % NaCl (w/v) at pH 7.0 and 28 °C, although it was able to grow at 4–32 °C and at pH values of 5.0–9.0. The NaCl tolerance range was from 0 to 15 %. The major respiratory quinone was Q8 but Q9 was also present. The most abundant fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C17 : 1 ω8c and C16 : 0. The polar lipids profile comprised phosphatidylglycerol and phosphatidylethanolamine as the most abundant representatives. Phylogenetic analyses confirmed the well- supported affiliation of strain RA15T within the genus Pseudoalteromonas, close to the type strains of Pseudoalteromonas neustonica, Pseudoalteromonas prydzensis and Pseudoalteromonas mariniglutinosa. Results of compara- tive phylogenetic and phenotypic studies between strain RA15T and its closest related species suggest that RA15T could be a new representative of the genus Pseudoalteromonas, for which the name Pseudoalteromonas rhizosphaerae sp. -
Pseudoalteromonas Aurantia] NCIMB 2033 As Pseudoalteromonas Flavipulchra Sp
International Journal of Systematic and Evolutionary Microbiology (2002), 52, 263–271 Printed in Great Britain Pseudoalteromonas maricaloris sp. nov., NOTE isolated from an Australian sponge, and reclassification of [Pseudoalteromonas aurantia] NCIMB 2033 as Pseudoalteromonas flavipulchra sp. nov. 1 Pacific Institute of Elena P. Ivanova,1† Ludmila S. Shevchenko,1 Tomoo Sawabe,2 Bioorganic Chemistry of 3 4 1 the Far-Eastern Branch of Anatolii M. Lysenko, Vasilii I. Svetashev, Nataliya M. Gorshkova, the Russian Academy of Masataka Satomi,5 Richard Christen6 and Valery V. Mikhailov1 Sciences, 690022 Vladivostok, pr. 100 Let Vladivostoku 159, Russia Author for correspondence: Elena P. Ivanova. Tel: j61 3 9214 5137. Fax: j61 3 9214 5050. 2 Laboratory of e-mail: eivanova!groupwise.swin.edu.au Microbiology, Faculty of Fisheries, Hokkaido University, 3-1-1 Minato- A marine, Gram-negative, aerobic bacterium that produced cytotoxic, lemon- cho, Hakodate 041-8611, yellow, chromopeptide pigments that inhibited the development of sea urchin Japan eggs has been isolated from the Australian sponge Fascaplysinopsis reticulata 3 Institute of Microbiology Hentschel. The cells of the organism were rod-shaped with a single polar of the Russian Academy of Sciences, 117811 Moscow, flagellum and they required NaCl for growth (05–10%) with optimum growth Russia at 1–3% NaCl. The temperature for growth was 10–37 SC, with optimum growth 4 Institute of Marine Biology at 25–30 SC. Growth occurred at pH values from 60to100, with optimum of the Far-Eastern Branch growth at pH 60–80. Major phospholipids were phosphatidylethanolamine, of the Russian Academy of phosphatidylglycerol and lyso-phosphatidylethanolamine. Of 26 fatty acids Sciences, 690041 Vladivostok, Russia with 11–19 carbon atoms that were detected, 16:1ω7, 16:0, 17:1ω8 and 18:1ω7 were predominant.