View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by St Andrews Research Repository 1 Short communication 2 3 Tandem affinity purification of exosome and replication factor C 4 complexes from the non-human infectious kinetoplastid parasite 5 Crithidia fasciculata 6 7 Wakisa Kipandula a, b, Terry K. Smith a and Stuart A. MacNeill a, * 8 9 a Biomedical Sciences Research Complex, University of St Andrews, North 10 Haugh, St Andrews, Fife KY16 9ST, UK. 11 b Department of Biomedical Sciences, College of Medicine, University of Malawi, 12 Private Bag 360, Chichiri, Blantyre 3, Malawi. 13 14 * Corresponding author: 15 Email:
[email protected] 16 Tel. (+44) 1334 467268 17 18 19 20 1 21 Abstract 22 23 Kinetoplastid parasites are responsible for a range of diseases with significant 24 global impact. Trypanosoma brucei and Trypanosoma cruzi cause human African 25 trypanosomiasis and Chagas disease, respectively, while various Leishmania 26 species are responsible for cutaneous, mucocutaneous and visceral 27 leishmaniasis. Understanding the biology of these organisms is key for effective 28 diagnosis, prophylaxis and treatment. The insect parasite Crithidia fasciculata 29 offers a safe and low-cost alternative for studies of kinetoplastid biology. C. 30 fasciculata does not infect humans, can be cultured to high yields in inexpensive 31 serum-free medium in a standard laboratory, and has a completely sequenced 32 publically available genome. Taking advantage of these features, however, 33 requires the adaptation of existing methods of analysis to C. fasciculata. Tandem 34 affinity purification is a widely used method that allows for the rapid purification of 35 intact protein complexes under native conditions.