TABLE OF CONTENTS

FOOD INTOLERANCE...... 03

AESKULISA® Food Intolerance Line...... 04

ELISA TESTING Kit Components...... 07

OVERVIEW...... 09 AESKULISA® Food Intolerance ONE G...... 09 ® AESKULISA Food Intolerance ONE G4...... 09 AESKULISA® Food Intolerance TWO G...... 09 ® AESKULISA Food Intolerance TWO G4...... 09 AESKULISA® Food Intolerance THREE G...... 09 ® AESKULISA Food Intolerance THREE G4...... 09 AESKULISA® Food Intolerance FOUR G...... 09 ® AESKULISA Food Intolerance FOUR G4...... 09

LISTS OF FOODS...... 10

PRODUCT HIGHLIGHTS...... 15

AESKUBLOTS® Food Intolerance Line...... 16

BLOT TESTING Kit Components...... 19

OVERVIEW...... 21 AESKUBLOTS® Food Intolerance ONE...... 22 AESKUBLOTS® Food Intolerance TWO...... 22 AESKUBLOTS® Food Intolerance THREE...... 23 AESKUBLOTS® Food Intolerance FOUR...... 23 AESKUBLOTS® Food Intolerance FIVE...... 24 AESKUBLOTS® Food Intolerance SIX...... 24 AESKUBLOTS® Food Intolerance SEVEN...... 24

LISTS OF FOODS...... 25

PRODUCT HIGHLIGHTS...... 29

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 01 FOOD INTOLERANCE

Food intolerance is defined as adverse reac- In case of immunogenic reactions, identify- tions to foods, beverages, food ingredients ing the critical foods can be very difficult. or food additives, which are not due to clas- The most accepted procedure is a mixture sical IgE-mediated food allergies. of elimination diet and stepwise provocation with individual food components. Symptoms of food intolerance reactions cover a wide spectrum and are not limited to Unfortunately it is quite tedious and lengthy, the digestive tract. While food allergy reac- usually performed in special clinics and can tions typically occur within minutes, food be inconclusive. So far, the mechanisms intolerance reactions are predominantly behind these adverse reactions are not well delayed type reactions. In addition, food understood. Nevertheless, the measure- intolerance reactions are rather unspecific, ment of IgG and IgG4 antibodies against food as intestinal cramps, flatulence, diarrhea or components can support and accelerate the constipation, urticaria, hives, headaches or identification of the foods that should be fatigue. omitted by the patients.

This makes it often very difficult to identify Our tests are very precise tools to detect relevant food components provoking the IgG respectively IgG4 antibodies in patients symptoms as well as the underlying patho- with suspected immunogenic food intoler- genic mechanisms, as they can be of very ance. They allow to test against a wide spec- different nature. trum of foods and food components at once. As we offer tests for several food panels, In many cases, food intolerance is due to a focused as well as wide spectrum screen- deficit in enzymes required to digest certain ings can be performed. In addition the prac- food components like sugars or histamine. titioner may choose a panel that covers the patient’s dietary habits and preferences. There can be underlying medical conditions resulting in adverse reactions to food, e.g. AESKULISA® and AESKUBLOTS® tests can by delaying food digestion or limiting absorp- be performed manually or automated, do tion of nutrients. just require only common laboratory devices and procedures. The AESKUCARE® test, a In other cases a patient may react very spe- POCT device, allows to test the blood of an cific to components of a certain food, like individual patient during patient’s visit at the special proteins, by immunological mecha- practitioner’s office, requiring just a few nisms. drops of blood from a finger prick.

In the first case, appropriate diagnostic pro- All our tests are CE-marked and are follow- cedures can be used to detect if a patient is ing the high diagnostic standards used in IgE lacking enzymes required to digest e.g. fruc- allergy testing, but are very specific for IgG tose or lactose, for example by a simple and IgG4 antibodies. breath test.

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 03 P A G 04 E

he food extracts we use in our ELISAs have been developed and optimized Tin-house, using state of the art tech- nologies. The allergens are prepared and purified from native sources as fresh pro- duce.

There are different kit configurations avail- able, addressing your specific diagnostic needs, allowing verifiability, flexibility and save costs: screening, profile and single ® assays. AESKULISA The screening kits contain such foods that quite often evoke food intolerance SIMPLIFIES YOUR ELISA ROUTINE reactions. Other kits allow to test against a wider spectrum of foods, covering also foods that are less common.

The AESKULISA® test line represents a variety of different immunoblots for efficient profile testing of food intolerances.

In profile kits each well of a row is coated with different food extracts and allow you to choose between quantitative or qualita- tive analysis.

There is also the possibility to measure only one parameter with our single kits, which are suitable for quantitative deter- mination or for confirmation of qualitative results, due to its high specificity.

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 05 ELISA TESTING Kit Components

Test principle

® •• AESKULISA for food specific IgG/IgG4 (slgG/sIgG4) is a conventional ELISA system (Enzyme Linked Immu- nosorbent Assay). •• On the surface of each cavity of the microtiter plate extracts of foods or food mixes are immobilized. •• For the test, a serum or plasma sample is diluted and transferred to the microtiter well.

•• slgG/sIgG 4 antibodies will bind to the corresponding antigens of the food extract.

•• Bound human sIgG/sIgG4 is detected by a specific horseradish peroxidase (HRP) conjugated anti-human

IgG/IgG4 antibody. After addition of the substrate TMB (3,3‘,5,5‘-Tetramethylbenzidine) bound sIgG/

sIgG4 antibodies are visualized by a blue coloration of the solution. •• Addition of stop solution interrupts the reaction and the blue color turns into yellow. •• The color intensity correlates with the amount of bound antibody and is quantified via photometric measurement.

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 07 P A G 08 E FOOD INTOLERANCE LINE OVERVIEW

Ref Product Format Food Iso- Calibration/ No. Name Extracts types Standard Range ® AESKULISA Food Intolerance Line AESKULISA® FOOD INTOLERANCE

Food intolerance reactions usually are less dangerous to AESKULISA® patients than the classic IgE-driven food allergies, but 330101 Food Intolerance 40 food f10, f120, f121, f14, f182, f187, f199, f20, f204, f206, f236, f24, IgG quantitative can also significantly reduce their quality of life. Usually ONE G extracts f25, f26, f27, f29, f309, f33, f35, f4, f40, f41, f44, f45, f46, f47, class 0–6 food intolerance reactions are delayed type reactions f48, f49, f50, f681, f7, f74, f78, f79, f83, f9, f955, f97, s15, s8 0.35–100 kU /L and can occur up to 3 days after food ingestion. This A makes it very difficult to identify the relevant food com- 330201 Food Intolerance 40 food f121, f13, f14, f158, f17, f199, f20, f24, f25, f26, f27, f29, f3, IgG4 quantitative ONE G4 extracts f300, f31, f33, f35, f36, f4, f40, f41, f44, f45, f47, f49, f5, f52, class 0–6 ponents and ingredients. To rule out enzyme deficien- f53, f65, f7, f74, f79, f83, f84, f85, f88, f89, f9, f955, s7 0.35–100 cies, breath tests can be used, and supervised elimina- kUA/L tion diets can verify the intolerance to a specific food. 330102 Food Intolerance 88 food f52, s1, f49, f168, f45, f29, s11, f30, f62, f182, f11, f94, f204, IgG quantitative TWO G extracts s14, f158, f12, f44, f13, f930, f200, f92, f950, f120, f7, f17, f21, class 0–6 IgG/IgG4 assays can be used as tools to support the practitioner in the identification process. f209, f164, f83, f3, f96, f31, f35, f78, f73, f84, f47, f163, f36, 0.35–100 f194, f24, f199, s3, f41, f88, f66, f65, s4, f121, f171, f20, f91, kUA/L s5, f33, f46, f86, f126, f53, f122, f141, f188, f9, f27, f5, f311, Food intolerance reactions have been described in chil- f325, s7, f26, f85, f89, f14, f132, f38, f40, s27, f25, f50, f143, s9, f74, f16, f15, f4, f300, s8, f32, f197, f48 dren and in adults of any age, and were reported for up 330202 Food Intolerance 88 food f52, s1, f49, f168, f45, f29, s11, f30, f62, f182, f11, f94, f204, IgG quantitative to a fifth of the population. 4 TWO G4 extracts s14, f158, f12, f44, f13, f930, f200, f92, f950, f120, f7, f17, f21, class 0–6 f209, f164, f83, f3, f96, f31, f35, f78, f73, f84, f47, f163, f36, 0.35–100

f194, f24, f199, s3, f41, f88, f66, f65, s4, f121, f171, f20, f91, kUA/L s5, f33, f46, f86, f126, f53, f122, f141, f188, f9, f27, f5, f311, f325, s7, f26, f85, f89, f14, f132, f38, f40, s27, f25, f50, f143, s9, f74, f16, f15, f4, f300, s8, f32, f197, f48 330103 Food Intolerance 96 food f10, f101, f103, f144, f145, f146, f149, f151, f152, f156, f162, IgG quantitative THREE G extracts f166, f167, f170, f173, f18, f181, f183, f185, f186, f187, f19, class 0–6 f191, f206, f207, f208, f210, f211, f214, f215, f216, f222, f233, 0.35–100

f267, f271, f288, f309, f312, f316, f319, f334, f34, f37, f39, kUA/L f411, f450, f56, f58, f6, f64, f69, f76, f77, f79, f80, f802, f804, f811, f812, f824, f827, f829, f832, f836, f837, f839, f840, f842, f843, f848, f855, f856, f876, f90, f947, f952, f955, f97, f98, f99, le001, le025, m5, s13, s15, s19, s2, s21, s22, s23, s24, s25, s26, s30, s6, s804

330203 Food Intolerance 96 food f10, f101, f103, f144, f145, f146, f149, f151, f152, f156, f162, IgG4 quantitative

THREE G4 extracts f166, f167, f170, f173, f18, f181, f183, f185, f186, f187, f19, class 0–6 f191, f206, f207, f208, f210, f211, f214, f215, f216, f222, f233, 0.35–100

f267, f271, f288, f309, f312, f316, f319, f334, f34, f37, f39, kUA/L f411, f450, f56, f58, f6, f64, f69, f76, f77, f79, f80, f802, f804, f811, f812, f824, f827, f829, f832, f836, f837, f839, f840, f842, f843, f848, f855, f856, f876, f90, f947, f952, f955, f97, f98, f99, le001, le025, m5, s13, s15, s19, s2, s21, s22, s23, s24, s25, s26, s30, s6, s804 330104 Food Intolerance 96 food f1, f128, f130, f142, f157, f159, f165, f169, f172, f174, f175, IgG quantitative FOUR G extracts f176, f177, f178, f180, f184, f189, f193, f196, f198, f201, f202, class 0–6 f203, f205, f212, f213, f217, f226, f231, f236, f241, f283, f284, 0.35–100

f287, f306, f307, f324, f327, f328, f329, f336, f338, f343, f406, kUA/L f412, f414, f461, f491, f55, f57, f651, f661, f681, f682, f71, f75, f791, f81, f818, f819, f82, f822, f838, f844, f845, f846, f847, f850, f851, f854, f859, f863, f867, f875, f881, f883, f902, f904, f911, f918, f919, f931, f934, f943, f948, f951, f953, f964, le020, le024, le026, le046, s10, s17, s32, s803

330204 Food Intolerance 96 food f1, f128, f130, f142, f157, f159, f165, f169, f172, f174, f175, IgG4 quantitative

FOUR G4 extracts f176, f177, f178, f180, f184, f189, f193, f196, f198, f201, f202, class 0–6 f203, f205, f212, f213, f217, f226, f231, f236, f241, f283, f284, 0.35–100

f287, f306, f307, f324, f327, f328, f329, f336, f338, f343, f406, kUA/L f412, f414, f461, f491, f55, f57, f651, f661, f681, f682, f71, f75, f791, f81, f818, f819, f82, f822, f838, f844, f845, f846, f847, f850, f851, f854, f859, f863, f867, f875, f881, f883, f902, Reference f904, f911, f918, f919, f931, f934, f943, f948, f951, f953, Turnbull J.L., Adams H.N., Gorard D.A., f964, le020, le024, le026, le046, s10, s17, s32, s803 Aliment Pharmacol Ther 2015; 41:3-25

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 09 P A G 10 E FOOD INTOLERANCE LINE Lists of Foods

® ® ® AESKULISA Food Intolerance ONE G AESKULISA Food Intolerance ONE G4 AESKULISA Food Intolerance TWO G and G4 No. Code English Name Scientific Name No. Code English Name Scientific Name No. Code English Name Scientific Name No. Code English Name Scientific Name 1 f10 Sesame Sesamum indicum 1 f121 Maize/Corn Zea mays 1 f52 Pineapple Ananas comosus 44 f41 Salmon Salmo salar 2 f120 Cucumber Cucumis sativus 2 f13 Peanut Arachis hypogaea 2 s1 Anise Pimpinella anisum 45 f88 Lamb Ovis aries 3 f121 Maize/Corn Zea mays 3 f14 Soy Glycine max 3 f49 Apple Malus domestica 46 f66 Leek Allium porrum 4 f14 Soy Glycine max 4 f158 Spelt Triticum aestivum 4 f168 Apricot Prunus armeniaca 47 f65 Lentil Lens culinaris ssp. spelta 5 f45 Baker's yeast Saccharomyces 48 s4 Bay leaf Laurus nobilis 5 f182 Broccoli Brassica oleracea 5 f17 Hazelnut Corylus avellana cerevisiae var. italica 49 f121 Maize/Corn Zea mays 6 f199 Cow’s milk Bos primigenius 6 f29 Banana Musa x paradisiaca 6 f187 Kale Brassica oleracea 50 f171 Mackerel Scomber scombrus f. taurus var. sabellica 7 s11 Sweet basil Ocimum basilicum 51 f20 Almond Prunus amygdalus 7 f20 Almond Prunus amygdalus 7 f199 Cow’s milk Bos primigenius 8 f30 Pear Pyrus communis 52 f91 Mango Mangifera indica 8 f24 Shrimp Penaeus monodon f. taurus 9 f62 Cauliflower Brassica oleracea 53 s5 Nutmeg apple Myristica fragrans 9 f25 Tomato Lycopersicon var. botrytis 8 f20 Almond Prunus amygdalus 54 f33 Orange Citrus sinensis esculentum 10 f182 Broccoli Brassica oleracea 9 f204 Cashew Anacardium 55 f46 Bell pepper Capsicum annuum occidentale 10 f26 Pork Sus scrofa var. italica 56 f86 Parsley Petroselinum 10 f206 Blueberry Vaccinium myrtillus domestica 11 f11 Buckwheat Fagopyrum 11 f27 Beef Bos primigenius crispum 11 f236 Cow whey Bos primigenius esculentum f. taurus 57 f126 Peppermint Mentha piperita f. taurus 12 f94 Camembert Bos primigenius 12 f29 Banana Musa x paradisiaca 58 f53 Peach Prunus persica 12 f24 Shrimp Penaeus monodon f. taurus 13 f3 Cod fish Gadus morhua 13 f204 Cashew nut Anacardium 59 f122 Plum Prunus domestica 13 f25 Tomato Lycopersicon occidentale esculentum 14 f300 Goat milk Capra hircus 60 f141 Button mushroom Agaricus bisporus 14 s14 Dill Anethum graveolens 14 f26 Pork Sus scrofa 15 f31 Carrot Daucus carota 61 f188 Small radish Raphanus sativus domestica 16 f33 Orange Citrus sinensis 15 f158 Spelt Triticum aestivum v. sativus ssp. spelta 15 f27 Beef Bos primigenius 17 f35 Potato Solanum tuberosum 62 f9 Rice Oryza sativa 16 f12 Pea, green Pisum sativum f. taurus 18 f36 Coconut Cocos nucifera 63 f27 Beef Bos primigenius 17 f44 Strawberry Fragaria vesca f. taurus 16 f29 Banana Musa x paradisiaca 19 f4 Wheat Triticum aestivum 18 f13 Peanut Arachis hypogaea 64 f5 Rye Secale cereale 17 f309 Chickpea Cicer arietinum 20 f40 Tuna Thunnus albacares 18 f33 Orange Citrus sinensis 19 f930 Trout Oncorhynchus 65 f311 Brussels sprouts Brassica oleracea 21 f41 Salmon Salmo salar var. gemmifera 19 f35 Potato Solanum tuberosum mykiss 22 f44 Strawberry Fragaria vesca 20 f200 Cheese, Gouda Bos primigenius 66 f325 Sheeps milk Ovis aries 20 f4 Wheat Triticum aestivum 23 f45 Baker’s yeast Saccharomyces f. taurus 67 s7 Pepper, black Piper nigrum 21 f40 Tuna Thunnus albacares cerevisiae 21 f92 Shaddock Citrus maxima 68 f26 Pork Sus scrofa 22 f41 Salmon Salmo salar 24 f47 Garlic Allium sativum 22 f950 Bean, green Phaseolus vulgaris domestica 23 f44 Strawberry Fragaria vesca 25 f49 Apple Malus domestica 23 f120 Cucumber Cucumis sativus 69 f85 Celery Apium graveolens 24 f45 Baker’s yeast Saccharomyces 26 f5 Rye Secale cereale 24 f7 Oat Avena sativa 70 f89 Mustard Sinapis alba cerevisiae 27 f52 Pineapple Ananas comosus 25 f17 Hazelnut Corylus avellana 71 f14 Soy Glycine max 25 f46 Bell pepper Capsicum annuum 28 f53 Peach Prunus persica 26 f21 Herring Clupea harengus 72 f132 Asparagus Asparagus officinalis 26 f47 Garlic Allium sativum 29 f65 Lentil Lens culinaris 27 f209 Raspberry Rubus idaeus 73 f38 Spinach Spinacia oleracea 27 f48 Onion Allium cepa 30 f7 Oat Avena sativa 28 f164 Millet Panicum miliaceum 74 f40 Tuna Thunnus albacares 28 f49 Apple Malus domestica 31 f74 Hen egg Gallus gallus 29 f83 Chicken Gallus gallus 75 s27 Common thyme Thymus vulgaris 29 f50 Grape Vitis vinifera domesticus domesticus 76 f25 Tomato Lycopersicon 30 f681 Red vine Vitis vinifera 32 f79 Gluten Triticum aestivum 30 f3 Cod Gadus morhua esculentum 31 f7 Oat Avena sativa 33 f83 Chicken Gallus gallus 31 f96 Camomile tea Matricaria 77 f50 Grape Vitis vinifera domesticus 32 f74 Hen egg Gallus gallus chamomilla 78 f143 Turkey Meleagris gallopavo domesticus 34 f84 Kiwi fruit Actinidia deliciosa 32 f31 Carrot Daucus carota 79 s9 Vanilla Vanilla planifolia 33 f78 Cow Casein Bos primigenius 35 f85 Celery Apium graveolens 33 f35 Potato Solanum tuberosum f. taurus 80 f74 Hen egg Gallus gallus 36 f88 Lamb Ovis aries 34 f78 Casein Bos primigenius domesticus 34 f79 Gluten Triticum aestivum 37 f89 Mustard Sinapis alba f. taurus 81 f16 Walnut Juglans regia 35 f83 Chicken Gallus gallus 38 f9 Rice Oryza sativa 35 f73 Sweet cherry Prunus avium domesticus 82 f15 White bean Phaseolus vulgaris 39 f955 Coffee Coffea arabica 36 f84 Kiwi fruit Actinidia deliciosa 36 f9 Rice Oryza sativa 83 f4 Wheat Triticum aestivum 40 s7 Pepper, black Piper nigrum 37 f47 Garlic Allium sativum 37 f955 Coffee Coffea arabica 84 f300 Goat milk Capra hircus 38 f163 Kohlrabi Brassica oleracea 38 f97 Cacao Theobroma cacao 85 s8 Cinnamon Cinnamomum verum var. gongylodes 39 s15 Ginger Zingiber officinale 86 f32 Lemon Citrus limon 39 f36 Coconut Cocos nucifera 40 s8 Cinnamon Cinnamomum verum 87 f197 Zucchini Cucurbita pepo 40 f194 Lettuce Lactuca sativa ssp. pepo convar. 41 f24 Shrimp Penaeus monodon giromontiina 42 f199 Cow’s milk Bos primigenius 88 f48 Onion Allium cepa f. taurus 43 s3 Caraway Carum carvi

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 11 P A G 12 E FOOD INTOLERANCE LINE Lists of Foods

® ® AESKULISA Food Intolerance THREE G and G4 AESKULISA Food Intolerance FOUR G and G4 No. Code English Name Scientific Name No. Code English Name Scientific Name No. Code English Name Scientific Name No. Code English Name Scientific Name 1 f10 Sesame Sesamum indicum 45 f411 Salmon roe Salmo salar 90 s23 Allspice Pimenta dioica 1 f1 Egg white Gallus gallus 2 f101 Swede Brassica napus ssp. 46 f450 Brewer's yeast Saccharomyces 91 s24 Rosemary Rosmarinus domesticus rapifera uvarum officinalis 2 f128 Grapevine snail Helix spec. 3 f103 Pecan nut Carya illinoinensis 47 f56 Perch Sebastes marinus 92 s25 Common sage Salvia officinalis 3 f130 Olive, black Olea europaea 4 f144 Locust bean gum Ceratonia siliqua 48 f58 Duck Anas platyrhynchos 93 s26 Chive Allium 4 f142 Soy milk Glycine max domestica schoenoprasum 5 f157 Pumpkin seed Cucurbita pepo 5 f145 Turbot Scophtalmus 49 f6 Barley Hordeum vulgare 94 s30 Cayenne pepper Capsicum maximus 6 f159 Durum wheat Triticum durum 50 f64 Cress Lepidium sativum frutescens 6 f146 Grapefruit Citrus paradisi 7 f165 Poppy seed Papaver somniferum 51 f69 Horseradish Armoracia rusticana 95 s6 Hot paprika powder Capsicum annuum 7 f149 Eggplant Solanum melongena 8 f169 Horse Equus caballus 52 f76 Alpha-lactalbumin Bos primigenius 96 s804 Tumeric Curcuma longa 8 f151 Guar Cyamopsis 9 f172 Anchovy Engraulis f. taurus tetragonolobus encrasicolus 53 f77 Beta-lactoglobulin Bos primigenius 9 f152 Plaice Pleuronectes 10 f174 Sole Solea solea f. taurus platessa 11 f175 Haddock Melanogrammus 54 f79 Gluten Triticum aestivum 10 f156 Sunflower seed Helianthus annuus aeglefinus 55 f80 Lobster Homarus gammarus 11 f162 Nectarine Prunus persica var. 12 f176 Squid Loligo spp. nucipersica 56 f802 Pollock Theragra 13 f177 Oyster Ostrea edulis chalcogramma 12 f166 Wild boar Sus scrofa 14 f178 Crayfish Procambarus clarkii 57 f804 Rabbit Oryctolagus 13 f167 Roe deer Capreolus capreolus 15 f180 Oyster mushroom Pleurotus ostreatus cuniculus 14 f170 Pike Esox lucius 58 f811 Amaranth Amaranthus 16 f184 Iceberg lettuce Lactuca sativa var. 15 f173 Sardine Sardina pilchardus caudatus capitata 16 f18 Brazil nut Bertholletia excelsa 59 f812 Olive, green Olea europaea 17 f189 Red cabbage Brassica oleracea capitata 17 f181 Avocado Persea americana 60 f824 Aspartam Aspartam 18 f193 Sauerkraut Brassica oleracea 18 f183 Chinese cabbage Brassica rapa ssp. 61 f827 Sweet lupine Lupinus albus conv. pekinensis 62 f829 Wild rice Zizania aquatica 19 f196 Savoy cabbage Brassica oleracea 19 f185 Endive Cichorium endiva 63 f832 Quinoa Chenopodium quinoa convar. capitata var. 20 f186 Fennel Foeniculum vulgare 64 f836 Honey pollen - sabauda var. azoricum 65 f837 Chicory Cichorium intybus 20 f198 Cow's milk, boiled Bos primigenius 21 f187 Kale Brassica oleracea var. foliosum f. taurus var. sabellica 66 f839 Lamb's lettuce Valerianella locusta 21 f201 Parmesan Bos primigenius 22 f19 Sweet chestnut Castanea sativa 67 f840 Chard Beta vulgaris ssp. f. taurus 23 f191 Beet root Beta vulgaris var. vulgaris 22 f202 Sheep milk cheese Ovis aries conditiva 68 f842 Sugar melon Cucumis melo var. 23 f203 Goat milk cheese Capra hircus 24 f206 Blueberry Vaccinium myrtillus cantalupensis 24 f205 Yoghurt Bos primigenius 25 f207 Blackberry Rubus fruticosus 69 f843 Chanterelle Cantharellus cibarius f. taurus 26 f208 Fig Ficus carica 70 f848 Green tea Camellia sinensis 25 f212 Ruhbarb Rheum rhabarbarum 27 f210 Red currant Ribes rubrum 71 f855 Lime blossom tea Tilia platyphylla 26 f213 Raisin Vitis vinifera 28 f211 Cranberry Vaccinium 72 f856 Maple syrup Acer saccharum 27 f217 Black currant Ribes nigrum macrocarpon 73 f876 Cream cheese Bos primigenius 28 f226 Cane sugar Saccharum 29 f214 Gooseberry Ribes uva-crispa f. taurus officinarum 30 f215 Cowberry Vaccinium vitis-idaea 74 f90 Malt Hordeum vulgare 29 f231 Great crab Cancer pagurus 31 f216 Pine nut, pignoles Pinus pinea 75 f947 Papaya Carica papaya 30 f236 Cow whey Bos primigenius 32 f222 Date Phoenix dactylifera 76 f952 Pumpkin Cucurbita maxima f. taurus 33 f233 Carp Cyprinus carpio 31 f241 Northern prawn Pandalus borealis 77 f955 Coffee Coffea arabica 34 f267 Cardamom Elettaria 32 f283 Buffalo milk Bubalus bubalis 78 f97 Cacao Theobroma cacao cardamomum 33 f284 Ostrich Struthio camelus 79 f98 Flax seed Linum usitatissimum 35 f271 Veal Bos primigenius 34 f287 Red kidney bean Phaseolus vulgaris f. taurus 80 f99 Black tea Camellia sinensis 35 f306 Lime Citrus limon 36 f288 Broad bean Vicia faba 81 le001 Glutamate - 36 f307 Hake Merluccius capensis 37 f309 Chickpea Cicer arietinum 82 le025 Agar - 37 f324 Perch Perca fluviatilis 38 f312 Roquette Eruca sativa 83 m5 Candida albicans Candida albicans 38 f327 European catfish Silurus glanis 39 f316 Rape seeds Brassica napus 84 s13 Coriander seed Coriandrum sativum 39 f328 Variegated scallop Chlamys varia 40 f319 Artichoke Cynara cardunculus 85 s15 Ginger Zingiber officinale 40 f329 Water melon Citrullus lanatus 41 f334 Hops Humulus lupulus 86 s19 Majoram Origanum majorana 41 f336 Wine vinegar Acetum vinegar 42 f34 Mandarine Citrus reticulata 87 s2 Curry powder - 42 f338 Scallop Pecten maximus 43 f37 Blue mussel Mytilus edulis 88 s21 Clove Syzygium 43 f343 Rose hip Rosa canina 44 f39 Cabbage Brassica oleracea aromaticum 44 f406 Aloe vera Aloe vera var. capitata 89 s22 Oregano Origanum vulgare

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 13 P A G 14 E PRODUCT HIGHLIGHTS Lists of Foods

Continued from page 13 ® ® Highlights of AESKULISA Immunoassays for the Diagnosis of AESKULISA Food Intolerance FOUR G and G4 No. Code English Name Scientific Name No. Code English Name Scientific Name Food Intolerances 45 f412 Lumpfish caviar Cycloperterus 85 f948 Passion fruit Passiflora edulis lumpus 86 f951 Bamboo shoot Bambusoideae 46 f414 Tilapia / red snapper Oreochromis 87 f953 Sweet potato Ipomoea batatas niloticus 88 f964 Liguorice Glycyrrhiza glabra 47 f461 Hot Pepper Capsicum 89 le020 Gelantine - frutescens A range of regional adaptable panels of ELISA plate layouts 48 f491 Pomegranate Punica granatum 90 le024 Pectin - 49 f55 Eel Anguilla anguilla 91 le026 Carrageenan - 50 f57 Goose Anser anser 92 le046 Xanthan - domesticus 93 s10 Pepper, white Piper nigrum 51 f651 Mung bean Vigna radiata 94 s17 Chili pepper Capsicum 52 f661 Bok choy Brassica rapa ssp. frutescens Preconfigured layouts, covering from 8 to 280 foods chinensis 95 s32 Lemon grass Cymbopogon 53 f681 Red vine Vitis vinifera citratus 54 f682 White vine Vitis vinifera 96 s803 Pepper, green Piper nigrum 55 f71 Spiny lobster Palinurus elephas 56 f75 Egg yolk Gallus gallus domesticus Quantitative results in commonly recognized “class 0–6” 57 f791 Gliadin Triticum aestivum 58 f81 Cheese, cheddar Bos primigenius type f. taurus 59 f818 Pistachio nut Pistacia vera 60 f819 Octopus Octopus vulgaris 61 f82 Blue cheese Bos primigenius f. taurus 18 month shelf life from date of manufacturing 62 f822 Tapioca Manihot esculenta 63 f838 Stinging nettle Urtica dioica 64 f844 Mushroom, type Lentinula edodes Shitake 65 f845 Verbena Aloysia citriodora 66 f846 Boletus Boletus edulis Automation friendly due to common reagents and protocols 67 f847 Rooibos tea Aspalathus linearis 68 f850 Sea buckthorn juice Hippophae rhamnoides 69 f851 Mirabelle Prunus domestica subsp. syriaca 70 f854 Parsnip Pastinaca sativa 71 f859 Mozzarella, buffalo Bubalus arnee Custom plate layouts available from a selection of more than 500 foods milk 72 f863 Cream Bos primigenius f. taurus 73 f867 Red deer Cervus elaphus 74 f875 Mozzarella Bos primigenius f. taurus 75 f881 Mutton Ovis aries For maximum flexibility and cost efficiency 76 f883 Cottage cheese Bos primigenius f. taurus 77 f902 Halibut Reinhardtius hippoglossoides 78 f904 Corn bran Zea mays 79 f911 Tofu Glycine max 80 f918 Rice, boiled Oryza sativa 81 f919 Rice, red Oryza sativa 82 f931 Hare Lepus europaeus 83 f934 Sword fish Xiphias gladius 84 f943 Litchi Litchi chinensis

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 15 P A G 16 E

ESKU’s complete in-house produc- tion philosophy for AESKUBLOTS® Aproducts, allows AESKU to have full control over the entire production chain, guaranteeing highest quality and unique product support.

Use of common reagents in all our food intolerance Immunoblots make ® AESKUBLOTS® ideal for automation, sav- AESKUBLOTS ing space, cost and time with optimized loading and reagents’ preparation. A STRATEGIC LINE-UP TO CHASE CLEAR RESULTS

For fast, easy and convenient auto- mated processing and evaluation of AESKUBLOTS® the HELIA® system can be used.

AESKUBLOTS® test line represents a variety of different immunoblots for efficient profile testing of food intolerances.

Easy semi-automatic processing is possi- ble with HELMED® BLOT processors, the results can then be processed either man- ually or automatically using the AESKU.SCAN software.

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 17 BLOT TESTING

Kit Components

Test principle •• Controls and up to 40 different antigens are bound in equispaced lines on the test strips. •• Serum sample from patient is added, during incubation specific antibodies of positive patients bind to the antigen. Afterwards, unbound substances are washed away. •• In a second incubation step, the specific antibodies are detected with an anti-

human IgG4 antibody conjugated with the enzyme horseradish peroxidase (HRP). Afterwards, unbound antibody conjugates are washed away. •• The strips are incubated with a HRP substrate solution. This will stain the lines that had been recognized by the patient’s antibodies with a blue color. Densi- tometric quantification of the color intensity allows to calculate the patient’s

sIgG4 level of each of the tested foods. The observed intensity of blue color is proportional to the antibody concentration in patients sample.

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 19 P A G 20 E FOOD INTOLERANCE LINE OVERVIEW

® Ref Product Format Antibody Ig Calibration/ AESKUBLOTS Food Intolerance Line No. Name Specifity Class Standard Range for quantitative detection of food specific IgG antibodies. A cost effective and reliable immunoassay. 4 FOOD INTOLERANCE AESKUBLOTS®

431401 Food Intolerance 24 tests f41, f3, f21, f24, f37, f26, f27, f83, f4, IgG4 quantitative More than 500 food extracts sorted into ONE 40 foods f5, f7, f8, f9, f11, f811, f31, f85, f39, class 0–6

four different groups are available: f47, f48, f12, f950, f14, f13, f25, f120, 0.35–100 kU A/L f35, f89, f1, f75, f199, f300, f325, f33, f29, f49, f84, f17, f16, f20

Sea food 431402 Food Intolerance 24 tests f41, f3, f152, f176, f37, f819, f26, f27, IgG4 quantitative Shrimp, crab, tuna, cuttlefish, salmon, cod TWO 40 foods f88, f4, f7, f10, f158, f811, f31, f120, class 0–6 fish, mackerel, sardine, oyster, blue mussel, f182, f47, f8, f39, f85, f827, f1, f75, 0.35–100 kUA /L scallop, i. a. f200, f78, f49, f33, f50, f53, f91, f20, f17, f13, f16, f818, f204, f35, f45, f14

431403 Food Intolerance 24 tests f40, f930, f802, f21, f177, f24, f58, IgG4 quantitative THREE 40 foods f83, f143, f5, f11, f6, f159, f164, f832, class 0–6

f79, f25, f48, f197, f812, f65, f12, 0.35–100 kUA /L f950, f199, f325, f300, f29, f84, f32, f44, f52, f156, f157, f89, s11, s15, Meat f955, f97, f141, f9 Pork, beef, sheep, lamb, goose, duck, chicken, 431404 Food Intolerance 24 tests f233, f171, f231, f411, f415, f412, f804, IgG4 quantitative turkey, rabbit, i. a. FOUR 40 foods f167, f62, f189, f952, f15, f842, f319, class 0–6

f188, f163, f66, f181, f69, f837, f142, 0.35–100 kUA /L f201, f94, f883, f203, f190, f873, f874, f876, f209, f122, f168, f30, f206, f73, f207, f34, f86, f267, s24 431405 Food Intolerance 24 tests f170, f173, f324, f902, f867, f271, quantitative Vegetables & fruits IgG4 Soy, beans, banana, apple, pineapple, FIVE 40 foods f186, f288, f38, f187, f149, f191, f132, class 0–6 f287, f310, f311, f947, f205, f875, f863, 0.35–100 kU /L strawberry, kiwi, raspberry, plum, i. a. A f810, f162, f210, f211, f146, f213, f217, f329, f103, f18, f36, f216, f345, s5, s27, s25, s13, f98, f19, f936

431406 Food Intolerance 24 tests f803, f327, f172, f935, f128, f57, f212, IgG4 quantitative SIX 40 foods f304, f312, f183, f843, f846, 180, class 0–6

f844, f306, f850, f943, f942, f948, 0.35–100 kUA /L f491, f343, f87, f124, f831, f236, f126, s12, s3, s7, s14, s9, s4, s8, f858, f841, s21, f165, f911, f334, f90 Nuts, dairy products, grains and others 431407 Food Intolerance 24 tests f145, f307, f174, f175, f55, f80, f338, IgG quantitative Peanut, almond, hazelnut, eggs, casein, 4 SEVEN 40 foods f169, f166, f572, f284, f309, f150, f92, class 0–6 milk, milk protein, pepper, spelt, oat, barley, f101, f196, f194, f838, f471, f272, f316, 0.35–100 kUA /L malt, buckwheat, amaranth, quinoa, sor- f481, f834, f222, f96, f99, f848, f651, ghum, yeast, ginger, i. a. f964, f226, f193, s23, s2, s31, s800, s802, f198, f283, f450, f791

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 21 P A G 22 E FOOD INTOLERANCE LINE

neg. pos. neg. pos. G G G G G Barcode Barcode Barcode Barcode 01 24 01 24 ® Function control Function control Function control Function control Negative control Negative control Negative control Negative control AESKUBLOTS Food Intolerance ONE Standard 1 Standard 1 Standard 1 Standard 1 Standard 2 Standard 2 Standard 2 Standard 2 Standard 3 Standard 3 Standard 3 Standard 3 S Tuna f40 Reference: 431401 C Trout f930 ® Pollock f802 S Herring f21 B AESKUBLOTS Food Intolerance THREE Oyster f177 Shrimp f24 Tests per Kit: 24 B Duck f58 C Chicken f83 Turkey f143 R Reference: 431403 Rye f5 Color Code: Green - Yellow O Buckwheat f11 Barley f6 R Durum wheat f159 B Tests per Kit: 24 Millet f164 A Quinoa f832 Conjugate: anti-human IgG4 C Gluten f79 C Tomato f25 C Onion f48 Color Code: Green - Green Zucchini f197 O Olive, green f812 Membrane based enzyme immunoassay for quantitative detection of food-spe- Lentil f65 B Pea, green f12 S Conjugate: anti-human IgG4 Bean, green f950 cific IgG4 antibodies in human serum or plasma. Antigens are located as parallel Milk f199 T Sheeps milk f325 C Goat milk f300 lines at exactly defined positions on a nitrocellulose membrane. The assay per- Banana f29 Membrane based enzyme immunoassay for quantitative detection of food-spe- Kiwi fruit f84 E Lemon f32 mits a large number of tests to be conducted in parallel over a short period of E Strawberry f44 cific IgG4 antibodies in human serum or plasma. Antigens are located as parallel Pineapple f52 Sunflower seed f156 time. The assay is used to support the diagnostic process in patients suspected S Pumpkin seed f157 O lines at exactly defined positions on a nitrocellulose membrane. The assay per- Mustard f89 B Sweet basil s11 to have food intolerances. A Ginger s15 K mits a large number of tests to be conducted in parallel over a short period of Coffee f955 Cacao f97 Button mushroom f141 A Rice f9 Function control Function control time. The assay is used to support the diagnostic process in patients suspected Function control Function control to have food intolerances.

neg. pos. neg. pos. G G Barcode

G Barcode G 01 24 ® Barcode Barcode Function control Function control 01 24 Negative control Negative control Food Intolerance FOUR Function control Function control AESKUBLOTS ® Standard 1 Standard 1 Negative control Negative control Standard 2 Standard 2 Standard 1 Standard 1 Standard 3 Standard 3 Standard 2 Standard 2 Food Intolerance TWO AESKUBLOTS Carp f233 Standard 3 Standard 3 Mackerel f171 Reference: 431404 S Great crab f231 C Salmon roe f411 Reference: 431402 Caviar black f415 S Lumpfish caviar f412 Tests per Kit: 24 B Rabbit f804 O Roe deer f167 Tests per Kit: 24 Cauliflower f62 B Red cabbage f189 L Pumpkin f952 Color Code: Green - Red White bean f15 O Sugar melon f842 S Color Code: Green - Grey Artichoke f319 S Small radish f188 Conjugate: anti-human IgG4 A Kohlrabi f163 C Leek f66 C Avocado f181 B Conjugate: anti-human IgG4 Horseradish f69 Chicory f837 Membrane based enzyme immunoassay for quantitative detection of food-spe- Soy milk f142 C Parmesan f201 C Membrane based enzyme immunoassay for quantitative detection of food-spe- Camembert f94 cific IgG4 antibodies in human serum or plasma. Antigens are located as parallel S Cottage cheese f883 E Goat milk cheese f203 E cific IgG4 antibodies in human serum or plasma. Antigens are located as parallel Edam cheese f190 lines at exactly defined positions on a nitrocellulose membrane. The assay per- C Cheese, Emmentaler type f873 C Cheese, Gruyere type f874 A Cream cheese f876 O lines at exactly defined positions on a nitrocellulose membrane. The assay per- Raspberry f209 mits a large number of tests to be conducted in parallel over a short period of Plum f122 Apricot f168 mits a large number of tests to be conducted in parallel over a short period of Pear f30 time. The assay is used to support the diagnostic process in patients suspected A Blueberry f206 Sweet cherry f73 time. The assay is used to support the diagnostic process in patients suspected Blackberry f207 to have food intolerances. Tangerine f34 Parsley f86 C to have food intolerances. Cardamom f267 Rosemary s24 B Function control Function control S Function control Function control

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 23 P A G 24 E neg. pos. FOOD INTOLERANCE LINE G G Barcode Barcode 01 24 ® Function control Function control Negative control Negative control Lists of Foods AESKUBLOTS Food Intolerance FIVE Standard 1 Standard 1 Standard 2 Standard 2 Standard 3 Standard 3 ® ® Reference: 431405 S AESKUBLOTS Food Intolerance ONE AESKUBLOTS Food Intolerance TWO R Tests per Kit: 24 F No. Code English Name Scientific Name No. Code English Name Scientific Name B S 1 f41 Salmon Salmo salar 1 f41 Salmon Salmo salar K Color Code: Green - Black E B 2 f3 Cod Gadus morhua 2 f3 Cod Gadus morhua A R 3 f21 Herring Clupea harengus 3 f152 Plaice Pleuronectes Conjugate: anti-human IgG4 B platessa 4 f24 Shrimp Penaeus monodon C 5 f37 Blue mussel Mytilus edulis 4 f176 Squid Loligo spp. Membrane based enzyme immunoassay for quantitative detection of food-spe- C N 5 f37 Blue mussel Mytilus edulis R 6 f26 Pork Sus scrofa cific IgG4 antibodies in human serum or plasma. Antigens are located as parallel C domestica 6 f819 Octopus Octopus vulgaris R lines at exactly defined positions on a nitrocellulose membrane. The assay per- B 7 f27 Beef Bos primigenius 7 f26 Pork Sus scrofa mits a large number of tests to be conducted in parallel over a short period of B f. taurus domestica C time. The assay is used to support the diagnostic process in patients suspected 8 f83 Chicken Gallus gallus 8 f27 Beef Bos primigenius N C domesticus f. taurus C to have food intolerances. C F 9 f4 Wheat Triticum aestivum 9 f88 Lamb Ovis aries S K 10 f5 Rye Secale cereale 10 f4 Wheat Triticum aestivum Function control Function control 11 f7 Oat Avena sativa 11 f7 Oat Avena sativa neg. pos. 12 f8 Maize Zea mays 12 f10 Sesame Sesamum indicum 13 f9 Rice Oryza sativa 13 f158 Spelt Triticum aestivum G G ssp. spelta 14 f11 Buckwheat Fagopyrum Barcode Barcode 01 24 esculentum 14 f811 Amaranth Amaranthus Function control Function control Negative control Negative control ® caudatus Standard 1 Standard 1 15 f811 Amaranth Amaranthus Standard 2 Standard 2 AESKUBLOTS Food Intolerance SIX Standard 3 Standard 3 caudatus 15 f31 Carrot Daucus carota Pikeperch f803 European catfish f327 Anchovy f172 16 f31 Carrot Daucus carota 16 f120 Cucumber Cucumis sativus Abalone f935 Reference: 431406 Grapevine snail f128 Goose f57 17 f85 Celery Apium graveolens 17 f182 Broccoli Brassica oleracea Rhubarb f212 Turnip f304 Tests per Kit: 24 18 f39 Cabbage Brassica oleracea var. italica Roquette f312 Chinese cabbage f183 18 f47 Garlic Allium sativum Chanterelle f843 var. capitata Boletus f846 Oyster mushroom f180 Color Code: Green - Magenta 19 f47 Garlic Allium sativum 19 f8 Maize Zea mays Mushroom, type Shitake f844 Lime f306 Sea buckthorn juice f850 20 f48 Onion Allium cepa 20 f39 Cabbage Brassica oleracea Litchi f943 Conjugate: anti-human IgG4 Persimon (kaki fruit, sharon) f942 var. capitata Passion fruit f948 21 f12 Pea, green Pisum sativum Pomegranate f491 Rose hip f343 22 f950 Bean, green Phaseolus vulgaris 21 f85 Celery Apium graveolens Honeydew melon f87 Membrane based enzyme immunoassay for quantitative detection of food-spe- Honey f124 22 f827 Sweet lupine Lupinus albus Kamut wheat f831 23 f14 Soy Glycine max Cow whey f236 cific IgG4 antibodies in human serum or plasma. Antigens are located as parallel Peppermint f126 23 f1 Egg white Gallus gallus Summer savory s12 24 f13 Peanut Arachis hypogaea Caraway s3 lines at exactly defined positions on a nitrocellulose membrane. The assay per- Pepper, black s7 domesticus Dill s14 25 f25 Tomato Lycopersicon Vanilla s9 mits a large number of tests to be conducted in parallel over a short period of 24 f75 Egg yolk Gallus gallus Bay leaf s4 esculentum Cinnamon s8 Lemon balm f858 domesticus Dandelion f841 time. The assay is used to support the diagnostic process in patients suspected 26 f120 Cucumber Cucumis sativus Clove s21 25 f200 Cheese, Gouda Bos primigenius Poppy seed f165 27 f35 Potato Solanum tuberosum Tofu f911 to have food intolerances. f. taurus Hops f334 Malt f90 28 f89 Mustard Sinapis alba Function control Function control 26 f78 Casein Bos primigenius 29 f1 Egg white Gallus gallus f. taurus domesticus 27 f49 Apple Malus domestica neg. pos. 30 f75 Egg yolk Gallus gallus 28 f33 Orange Citrus sinensis domesticus G G 29 f50 Grape Vitis vinifera Barcode Barcode 31 f199 Cow’s milk Bos primigenius 01 24 30 f53 Peach Prunus persica Function control Function control f. taurus ® Negative control Negative control Standard 1 Standard 1 31 f91 Mango Mangifera indica AESKUBLOTS Food Intolerance SEVEN Standard 2 Standard 2 32 f300 Goat milk Capra hircus Standard 3 Standard 3 T 32 f20 Almond Prunus amygdalus 33 f325 Sheep’s milk Ovis aries S Reference: 431407 33 f17 Hazelnut Corylus avellana E 34 f33 Orange Citrus sinensis L 34 f13 Peanut Arachis hypogaea S 35 f29 Banana Musa x paradisiaca Tests per Kit: 24 35 f16 Walnut Juglans regia 36 f49 Apple Malus domestica O C 36 f818 Pistachio nut Pistacia vera Color Code: Green - Cyan C 37 f84 Kiwi fruit Actinidia deliciosa S S 37 f204 Cashew nut Anacardium S 38 f17 Hazelnut Corylus avellana Conjugate: anti-human IgG4 L occidentale S 39 f16 Walnut Juglans regia B T 38 f35 Potato Solanum tuberosum R 40 f20 Almond Prunus amygdalus Membrane based enzyme immunoassay for quantitative detection of food-spe- S T 39 f45 Baker’s yeast Saccharomyces D cific IgG4 antibodies in human serum or plasma. Antigens are located as parallel C cerevisiae B 40 f14 Soy Glycine max lines at exactly defined positions on a nitrocellulose membrane. The assay per- L C S mits a large number of tests to be conducted in parallel over a short period of A C C time. The assay is used to support the diagnostic process in patients suspected C C to have food intolerances. B B Function control Function control

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 25 P A G 26 E FOOD INTOLERANCE LINE Lists of Foods Lists of Foods

AESKUBLOTS® Food Intolerance THREE AESKUBLOTS® Food Intolerance FOUR AESKUBLOTS® Food Intolerance FIVE AESKUBLOTS® Food Intolerance SIX No. Code English Name Scientific Name No. Code English Name Scientific Name No. Code English Name Scientific Name No. Code English Name Scientific Name 1 f40 Tuna Thunnus albacares 1 f233 Carp Cyprinus carpio 1 f170 Pike Esox lucius 1 f803 Pikeperch Sander lucioperca 2 f930 Trout Oncorhynchus 2 f171 Mackerel Scomber scombrus 2 f173 Sardine Sardina pilchardus 2 f327 European catfish Silurus glanis mykiss 3 f231 Great crab Cancer pagurus 3 f324 Perch Perca fluviatilis 3 f172 Anchovy Engraulis 3 f802 Pollock Theragra 4 f411 Salmon roe Salmo salar 4 f902 Halibut Reinhardtius encrasicolus chalcogramma 5 f415 Caviar black Acipenseridae hippoglossoides 4 f935 Abalone Haliotis japonica 4 f21 Herring Clupea harengus 6 f412 Lumpfish caviar Cycloperterus 5 f867 Red deer Cervus elaphus 5 f128 Grapevine snail Helix spec. 5 f177 Oyster Ostrea edulis lumpus 6 f271 Veal Bos primigenius 6 f57 Goose Anser anser 6 f24 Shrimp Penaeus monodon 7 f804 Rabbit Oryctolagus f. taurus domesticus 7 f58 Duck Anas platyrhynchos cuniculus 7 f186 Fennel Foeniculum vulgare 7 f212 Rhubarb Rheum rhabarbarum domestica 8 f167 Roe deer Capreolus capreolus var. azoricum 8 f304 Turnip Brassica rapa subsp. 8 f83 Chicken Gallus gallus 9 f62 Cauliflower Brassica oleracea 8 f288 Broad bean Vicia faba rapa domesticus var. botrytis 9 f38 Spinach Spinacia oleracea 9 f312 Roquette Eruca sativa 9 f143 Turkey Meleagris gallopavo 10 f189 Red cabbage Brassica oleracea 10 f187 Kale Brassica oleracea 10 f183 Chinese cabbage Brassica rapa ssp. 10 f5 Rye Secale cereale capitata var. sabellica pekinensis 11 f11 Buckwheat Fagopyrum 11 f952 Pumpkin Cucurbita maxima 11 f149 Eggplant Solanum melongena 11 f843 Chanterelle Cantharellus cibarius esculentum 12 f15 White bean Phaseolus vulgaris 12 f191 Beet root Beta vulgaris var. 12 f846 Boletus Boletus edulis 12 f6 Barley Hordeum vulgare 13 f842 Sugar melon Cucumis melo var. conditiva 13 f180 Oyster mushroom Pleurotus ostreatus 13 f159 Durum wheat Triticum durum cantalupensis 13 f132 Asparagus Asparagus officinalis 14 f844 Mushroom, type Lentinula edodes 14 f164 Millet Panicum miliaceum 14 f319 Artichoke Cynara cardunculus 14 f287 Red kidney bean Phaseolus vulgaris Shitake 15 f832 Quinoa Chenopodium quinoa 15 f188 Small radish Raphanus sativus v. 15 f310 White radish Raphanus sativus 15 f306 Lime Citrus limon 16 f79 Gluten Triticum aestivum sativus 16 f311 Brussels sprouts Brassica oleracea 16 f850 Sea buckthorn juice Hippophae 17 f25 Tomato Lycopersicon 16 f163 Kohlrabi Brassica oleracea var. gemmifera rhamnoides esculentum var. gongylodes 17 f947 Papaya Carica papaya 17 f943 Litchi Litchi chinensis 18 f48 Onion Allium cepa 17 f66 Leek Allium porrum 18 f205 Yoghurt Bos primigenius 18 f942 Persimon (kaki fruit, Diospyros kaki 19 f197 Zucchini Cucurbita pepo 18 f181 Avocado Persea americana f. taurus sharon) ssp. pepo convar. 19 f69 Horseradish Armoracia rusticana 19 f875 Mozzarella Bos primigenius 19 f948 Passion fruit Passiflora edulis giromontiina 20 f837 Chicory Cichorium intybus f. taurus 20 f491 Pomegranate Punica granatum 20 f812 Olive, green Olea europaea var. foliosum 20 f863 Cream Bos primigenius 21 f343 Rose hip Rosa canina f. taurus 21 f65 Lentil Lens culinaris 21 f142 Soy milk Glycine max 22 f87 Honeydew melon Cucumis melo var. 22 f12 Pea, green Pisum sativum 22 f201 Parmesan Bos primigenius 21 f810 Curd cheese Bos primigenius inodorus f. taurus 23 f950 Bean, green Phaseolus vulgaris f. taurus 23 f124 Honey - 22 f162 Nectarine Prunus persica var. 23 f94 Camembert Bos primigenius 24 f831 Kamut wheat Triticum polonicum 24 f199 Cow’s milk Bos primigenius nucipersica f. taurus f. taurus 23 f210 Red currant Ribes rubrum 25 f236 Cow whey Bos primigenius 25 f325 Sheep’s milk Ovis aries 24 f883 Cottage cheese Bos primigenius f. taurus f. taurus 24 f211 Cranberry Vaccinium 26 f300 Goat milk Capra hircus 26 f126 Peppermint Mentha piperita 25 f203 Goat milk cheese Capra hircus macrocarpon 27 f29 Banana Musa x paradisiaca 27 s12 Summer savory Satureja hortensis 26 f190 Edam cheese Bos primigenius 25 f146 Grapefruit Citrus paradisi 28 s3 Caraway Carum carvi 28 f84 Kiwi fruit Actinidia deliciosa f. taurus 26 f213 Raisin Vitis vinifera 29 f32 Lemon Citrus limon 27 f873 Cheese, Emmentaler Bos primigenius 27 f217 Black currant Ribes nigrum 29 s7 Pepper, black Piper nigrum 30 f44 Strawberry Fragaria vesca type f. taurus 28 f329 Water melon Citrullus lanatus 30 s14 Dill Anethum graveolens 31 f52 Pineapple Ananas comosus 28 f874 Cheese, Gruyere Bos primigenius 29 f103 Pecan nut Carya illinoinensis 31 s9 Vanilla Vanilla planifolia type f. taurus 32 f156 Sunflower seed Helianthus annuus 30 f18 Brazil nut Bertholletia excelsa 32 s4 Bay leaf Laurus nobilis 29 f876 Cream cheese Bos primigenius 33 f157 Pumpkin seed Cucurbita pepo 31 f36 Coconut Cocos nucifera 33 s8 Cinnamon Cinnamomum verum f. taurus 34 f89 Mustard Sinapis alba 34 f858 Lemon balm Melissa officinalis 30 f209 Raspberry Rubus idaeus 32 f216 Pine nut, pignoles Pinus pinea 35 s11 Sweet basil Ocimum basilicum 35 f841 Dandelion Taraxacum vulgare 31 f122 Plum Prunus domestica 33 f345 Macadamia nut Macadamia ternifolia 36 s15 Ginger Zingiber officinale 34 s5 Nutmeg apple Myristica fragrans 36 s21 Clove Syzygium 32 f168 Apricot Prunus armeniaca aromaticum 37 f955 Coffee Coffea arabica 35 s27 Common thyme Thymus vulgaris 33 f30 Pear Pyrus communis 37 f165 Poppy seed Papaver somniferum 38 f97 Cacao Theobroma cacao 36 s25 Common sage Salvia officinalis 34 f206 Blueberry Vaccinium myrtillus 38 f911 Tofu Glycine max 39 f141 Button mushroom Agaricus bisporus 37 s13 Coriander seed Coriandrum sativum 35 f73 Sweet cherry Prunus avium 39 f334 Hops Humulus lupulus 40 f9 Rice Oryza sativa 38 f98 Flax seed Linum usitatissimum 36 f207 Blackberry Rubus fruticosus 40 f90 Malt Hordeum vulgare 37 f34 Tangerine Citrus reticulata 39 f19 Sweet chestnut Castanea sativa 38 f86 Parsley Petroselinum 40 f936 Kelp Saccharina japonica crispum 39 f267 Cardamom Elettaria cardamomum 40 s24 Rosemary Rosmarinus officinalis

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 27 P A G 28 E PRODUCT HIGHLIGHTS Lists of Foods

® AESKUBLOTS Food Intolerance SEVEN Highlights of AESKUBLOTS® Line Immunoassays specific check-up of No. Code English Name Scientific Name 1 f145 Turbot Scophtalmus Food Intolerances maximus 2 f307 Hake Merluccius capensis 3 f174 Sole Solea solea 4 f175 Haddock Melanogrammus aeglefinus 5 f55 Eel Anguilla anguilla 6 f80 Lobster Homarus gammarus Automation friendly due to common reagents and protocols 7 f338 Scallop Pecten maximus 8 f169 Horse Equus caballus 9 f166 Wild boar Sus scrofa 10 f572 Quail Coturnix coturnix 11 f284 Ostrich Struthio camelus Quantitative results in kUA/L or classes 12 f309 Chickpea Cicer arietinum 13 f150 Carob Ceratonia siliqua 14 f92 Shaddock Citrus maxima 15 f101 Swede Brassica napus ssp. rapifera 16 f196 Savoy cabbage Bos primigenius Cost-effective screening and differential diagnostics f. taurus 17 f194 Lettuce Lactuca sativa 18 f838 Stinging nettle Urtica dioica 19 f471 Bear's garlic Allium ursinum 20 f272 Tarragon Artemisia dracunculus Testing of multiple foods per test 21 f316 Rape seeds Brassica napus 22 f481 Shallot Allium ascalonicum 23 f834 Tigernut Cyperus esculentus 24 f222 Date Phoenix dactylifera 25 f96 Camomile tea Matricaria chamomilla Calibrators and controls included on the strip 26 f99 Black tea Camellia sinensis 27 f848 Green tea Camellia sinensis 28 f651 Mung bean Vigna radiata 29 f964 Liguorice Glycyrrhiza glabra 30 f226 Cane sugar Saccharum officinarum Wide range of panels 31 f193 Sauerkraut Brassica oleracea conv. 32 s23 Allspice Pimenta dioica 33 s2 Curry powder - 34 s31 Cumin Cuminum cyminum 35 s800 Capers Capparis spinosa 36 s802 Catmint Nepeta catania 37 f198 Cow’s milk, boiled Bos primigenius f. taurus 38 f283 Buffalo milk Bubalus bubalis 39 f450 Brewer’s yeast Saccharomyces uvarum 40 f791 Gliadin Triticum aestivum

P A G AESKU.GROUP • FOOD INTOLERANCE • VERSION 017-2019-06-17 E 29 NOTES IMPRINT

Publisher AESKU.GROUP GmbH & Co. KG Mikroforum Ring 2-3 55234 Wendelsheim Germany

Managing Director: Dr. Torsten Matthias District Court Mainz HRA41796

Telephone: +49-6734-9622-0 Telefax: +49-6734-9622-2222

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Last Update June 17, 2019