Mouse Model of Endemic Burkitt Translocations Reveals the Long-Range Boundaries of Ig-Mediated Oncogene Deregulation

Total Page:16

File Type:pdf, Size:1020Kb

Mouse Model of Endemic Burkitt Translocations Reveals the Long-Range Boundaries of Ig-Mediated Oncogene Deregulation Mouse model of endemic Burkitt translocations reveals the long-range boundaries of Ig-mediated oncogene deregulation Alexander L. Kovalchuka,1, Camilo Ansarah-Sobrinhob,1,Ofir Hakimc,1, Wolfgang Reschb, Helena Tolarováb, Wendy Duboisd, Arito Yamaneb, Makiko Takizawab, Isaac Kleinf, Gordon L. Hagerc, Herbert C. Morse IIIa, Michael Potterd,1, Michel C. Nussenzweige,f,2, and Rafael Casellasb,g,2 aLaboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852; bGenomics and Immunity, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892; cLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892; dLaboratory of Cancer Biology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892; eLaboratory of Molecular Immunology, and fHoward Hughes Medical Institute, The Rockefeller University, New York, NY 10065; and gCenter of Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 Edited by Klaus Rajewsky, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany, and approved May 22, 2012 (received for review January 5, 2012) Human Burkitt lymphomas are divided into two main clinical human Burkitt lymphomas (BLs), mouse plasmacytomas (PCTs), variants: the endemic form, affecting African children infected with and rat immunocytomas (15, 16). malaria and the Epstein-Barr virus, and the sporadic form, distrib- Human BLs are divided into two main clinical variants: the uted across the rest of the world. However, whereas sporadic endemic form, affecting African children infected with malaria translocations decapitate Myc from 5′ proximal regulatory ele- and the Epstein-Barr virus, and the sporadic form, distributed ments, most endemic events occur hundreds of kilobases away across the rest of the world (17). Although both tumors are from Myc. The origin of these rearrangements and how they de- driven primarily by Igh/Myc rearrangements (18), the precise regulate oncogenes at such distances remain unclear. We here location of the breakpoints differs in the two subsets. In sporadic recapitulate endemic Burkitt lymphoma-like translocations in BLs (and mouse PCTs), translocations occur within Myc exon – ′ IMMUNOLOGY plasmacytomas from uracil N-glycosylase and activation-induced 1 intron 1, and as such they decapitate the oncogene from its 5 cytidine deaminase-deficient mice. Mapping of translocation regulatory elements (19). These canonical rearrangements result – breakpoints using an acetylated histone H3 lysine 9 chromatin from AID activity at IgS (during CSR) as well as at Myc (20 23). immunoprecipitation sequencing approach reveals Igh fusions up In contrast, endemic BL translocations are noncanonical in that ∼ they map hundreds of kilobases upstream from Myc basal to 350 kb upstream of Myc or the related oncogene Mycn.A – comprehensive analysis of epigenetic marks, PolII recruitment, promoters (24 28). Because these lesions leave Myc upstream and transcription in tumor cells demonstrates that the 3′ Igh en- promoter elements intact, it is unclear how they bring about hancer (Eα) vastly remodels ∼450 kb of chromatin into translo- oncogene deregulation. The etiology of these events is also un- cated sequences, leading to significant polymerase occupancy known, primarily because to date endemic BL-like translocations and constitutive oncogene expression. We show that this long- have not been systematically reproduced in the mouse. Because of their proximity to V(D)J genes, endemic BL range epigenetic reprogramming is directly proportional to the rearrangements have been proposed to arise as by-products of physical interaction of Eα with translocated sites. Our studies thus SHM but not of CSR (19, 27, 29). On the basis of this idea and in uncover the extent of epigenetic remodeling by Ig 3′ enhancers an attempt to recapitulate endemic translocations in the mouse, and provide a rationale for the long-range deregulation of translo- we combined a UNG null allele (30) with the Bcl-xL transgene − − cated oncogenes in endemic Burkitt lymphomas. The data also shed that produces plasma cell tumors (31). In UNG / B cells, AID light on the origin of endemic-like chromosomal rearrangements. hypermutation remains active as evidenced by high levels of C-to-T mutation (32). In contrast, CSR (32, 33) and canonical chromosome conformation capture | chromosome translocations | Igh/Myc translocations (34) are impaired because uracyl deglyco- activation-induced cytidine deamination | epigenetics sylation is an important intermediate step in double-strand DNA − − (dsDNA) break formation (12). The UNG / mouse thus might be uring B-cell responses to antigen, somatic hypermutation an ideal model to recapitulate rare, noncanonical translocations. D(SHM) introduces point mutations at variable (V) genes, a Consistent with a primary role for AID and UNG in the CSR process that, when coupled to clonal selection, can enhance the breaks mediating Igh/Myc translocations (20, 23, 34), we find that, antibody’saffinity for the antigen (1, 2). Class switch re- in the absence of UNG, the incidence of B-cell transformation is − − combination (CSR) is a second type of B-lymphocyte–specific markedly delayed. Strikingly, the vast majority of UNG / and − − DNA modification reaction that results in diversification of an- AID / tumors that do develop carry endemic BL-like trans- tibody effector functions by replacing the Igh constant domain locations with breakpoints accumulating over hundreds of kilo- Cμ with one of a set of downstream genes: Cγ,Cɛ,orCα (3–5). bases upstream of Myc or its homolog the Mycn oncogene. Both SHM and CSR are initiated by activation-induced cytidine Analysis of these tumors by deep-sequencing techniques sheds deaminase (AID) (6, 7), a B-cell–specific enzyme that deami- nates cytidine residues to produce uracils (8). Multiple repair pathways including uracil DNA glycosylase (UNG), mismatch Author contributions: A.L.K., G.L.H., H.C.M., M.C.N., and R.C. designed research; A.L.K., repair enzymes, and error-prone polymerases process U:G mis- C.A.-S., O.H., H.T., W.D., A.Y., M.T., and I.K. performed research; W.R., M.P., and R.C. matches into SHM or DNA double-strand breaks intermediate analyzed data; and M.P. and R.C. wrote the paper. to CSR (3–5, 9). The authors declare no conflict of interest. Although most AID-mediated lesions result in SHM or CSR, This article is a PNAS Direct Submission. they can occasionally generate chromosome deletions, trans- 1A.L.K., C.A.-S., O.H., and M.P. contributed equally to this work. – locations, and aneuploidy (10 14). These genomic abnormalities 2To whom correspondence may be addressed. E-mail: [email protected] or nussen@ have the potential to deregulate oncogenes and thereby promote rockefeller.edu. cell transformation. Perhaps the best-studied example is the This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10. translocation that juxtaposes Igh to the Myc proto-oncogene in 1073/pnas.1200106109/-/DCSupplemental. www.pnas.org/cgi/doi/10.1073/pnas.1200106109 PNAS Early Edition | 1of6 Downloaded by guest on October 2, 2021 light on both the origin of endemic-like translocations and the mouse lines for canonical T(12;15)s by long-distance PCR. As genomic features that impact their tumor capacity. previously described in wild-type mice (31), UNG+/+Bcl-xL and − UNG+/ Bcl-xL accumulated T(12:15)s following pristane injection. Results From 264 DNA samples analyzed for each strain, we isolated 39 − − +/+ UNG / PCTs Lack Canonical Igh/Myc Translocations. UNG deficiency (14.8%) and 36 (13.6%) distinct translocations from UNG +/− has been shown to profoundly impair CSR and canonical Igh/ Bcl-xL and UNG Bcl-xL mice, respectively (Fig. 1C). In con- Myc translocations in LPS+IL-4–stimulated B cells in vitro (34) trast, we failed to detect canonical T(12;15)s in any of the 216 −/− without significantly affecting SHM (32, 33) (Fig. S1). To in- DNAs isolated from UNG Bcl-xL lymphoid tissues or oil vestigate how this phenotype might impact the genesis of chro- granulomas (n = 216, Fig. 1C). This discrepancy, which is con- − − − mosomal translocations in vivo, UNG+/+, UNG+/ , and UNG / sistent with previous ex vivo translocation studies (34), may be mice bearing the Bcl-xL transgene were given one (day 0) or two explained by the fact that dsDNA break formation at switch (days 0 and 60) injections of the mineral oil pristane in the regions is decreased in the absence of UNG (35) (Fig. S2). The peritoneal cavity. Tumor development, which occurs in oil results therefore demonstrate that plasmacytomagenesis in granulomas, was diagnosed by the appearance of transformed Bcl-xL transgenic mice that lack UNG resembles that observed − −/− plasma cells in the ascites. UNG+/+ and UNG+/ mice rapidly in AID mice in that it is not associated with canonical Igh/ developed plasmacytomas with no apparent difference in kinet- Myc translocations. ics (Fig. 1A). In contrast, plasmacytomagenesis was inefficient in − − −/− the absence of UNG. Compared with wild-type controls, UNG / UNG Translocations Preferentially Localize Upstream of Myc Tran- −/− tumors developed with lower frequency (18% vs. 90% at day 220, scription Start Site. To explore whether UNG tumors carried P < 0.0005) and longer mean latency (136 vs. 73 d, Fig. 1A). noncanonical forms of T(12:15)s or other translocations, we next Consistent with previous findings (31), UNG+/+Bcl-xL mice applied FISH and spectral karyotyping (SKY). Despite our in- −/− developed multiple foci of premalignant, hyperchromatic plasma ability to amplify T(12:15)s by PCR, 9 of the 11 UNG tumors cells harboring Igh/Myc translocations [T(12;15)s] as early as 10 d carried rearrangements between chromosomes 12 and 15 (Fig. post pristane injection, whereas their appearance was delayed 2A and Fig. S3). To precisely map the translocation breakpoints, and their number reduced in the absence of UNG [7% (3 of 45 we made use of high-resolution array comparative genomic hy- mice analyzed), Fig.
Recommended publications
  • Functional Roles of Bromodomain Proteins in Cancer
    cancers Review Functional Roles of Bromodomain Proteins in Cancer Samuel P. Boyson 1,2, Cong Gao 3, Kathleen Quinn 2,3, Joseph Boyd 3, Hana Paculova 3 , Seth Frietze 3,4,* and Karen C. Glass 1,2,4,* 1 Department of Pharmaceutical Sciences, Albany College of Pharmacy and Health Sciences, Colchester, VT 05446, USA; [email protected] 2 Department of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405, USA; [email protected] 3 Department of Biomedical and Health Sciences, University of Vermont, Burlington, VT 05405, USA; [email protected] (C.G.); [email protected] (J.B.); [email protected] (H.P.) 4 University of Vermont Cancer Center, Burlington, VT 05405, USA * Correspondence: [email protected] (S.F.); [email protected] (K.C.G.) Simple Summary: This review provides an in depth analysis of the role of bromodomain-containing proteins in cancer development. As readers of acetylated lysine on nucleosomal histones, bromod- omain proteins are poised to activate gene expression, and often promote cancer progression. We examined changes in gene expression patterns that are observed in bromodomain-containing proteins and associated with specific cancer types. We also mapped the protein–protein interaction network for the human bromodomain-containing proteins, discuss the cellular roles of these epigenetic regu- lators as part of nine different functional groups, and identify bromodomain-specific mechanisms in cancer development. Lastly, we summarize emerging strategies to target bromodomain proteins in cancer therapy, including those that may be essential for overcoming resistance. Overall, this review provides a timely discussion of the different mechanisms of bromodomain-containing pro- Citation: Boyson, S.P.; Gao, C.; teins in cancer, and an updated assessment of their utility as a therapeutic target for a variety of Quinn, K.; Boyd, J.; Paculova, H.; cancer subtypes.
    [Show full text]
  • Quantitative Proteomics Methods for the Analysis of Histone Post-Translational Modifications
    Université de Montréal Quantitative Proteomics Methods for the Analysis of Histone Post-translational Modifications par Nebiyu Ali Abshiru Département de Chimie Faculté des arts et des sciences Thèse présentée à la Faculté des études supérieures et postdoctorales en vue de l’obtention du grade de philosophiae doctor (Ph.D.) en chimie Septembre 2015 ©Nebiyu Ali Abshiru, 2015 i Résumé Les histones sont des protéines nucléaires hautement conservées chez les cellules des eucaryotes. Elles permettent d’organiser et de compacter l’ADN sous la forme de nucléosomes, ceux-ci representant les sous unités de base de la chromatine. Les histones peuvent être modifiées par de nombreuses modifications post-traductionnelles (PTMs) telles que l’acétylation, la méthylation et la phosphorylation. Ces modifications jouent un rôle essentiel dans la réplication de l’ADN, la transcription et l’assemblage de la chromatine. L’abondance de ces modifications peut varier de facon significative lors du developpement des maladies incluant plusieurs types de cancer. Par exemple, la perte totale de la triméthylation sur H4K20 ainsi que l’acétylation sur H4K16 sont des marqueurs tumoraux spécifiques a certains types de cancer chez l’humain. Par conséquent, l’étude de ces modifications et des événements determinant la dynamique des leurs changements d’abondance sont des atouts importants pour mieux comprendre les fonctions cellulaires et moléculaires lors du développement de la maladie. De manière générale, les modifications des histones sont étudiées par des approches biochimiques telles que les immuno-buvardage de type Western ou les méthodes d’immunoprécipitation de la chromatine (ChIP). Cependant, ces approches présentent plusieurs inconvénients telles que le manque de spécificité ou la disponibilité des anticorps, leur coût ou encore la difficulté de les produire et de les valider.
    [Show full text]
  • The Determinants of Nucleosome Patterns and the Impact of Phosphate Starvation on Nucleosome Patterns and Gene Expression in Rice
    Louisiana State University LSU Digital Commons LSU Doctoral Dissertations Graduate School 3-14-2018 The etD erminants of Nucleosome Patterns and the Impact of Phosphate Starvation on Nucleosome Patterns and Gene Expression in Rice Qi Zhang Louisiana State University and Agricultural and Mechanical College, [email protected] Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_dissertations Part of the Agronomy and Crop Sciences Commons, Bioinformatics Commons, Genetics Commons, Genomics Commons, and the Plant Breeding and Genetics Commons Recommended Citation Zhang, Qi, "The eD terminants of Nucleosome Patterns and the Impact of Phosphate Starvation on Nucleosome Patterns and Gene Expression in Rice" (2018). LSU Doctoral Dissertations. 4502. https://digitalcommons.lsu.edu/gradschool_dissertations/4502 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Doctoral Dissertations by an authorized graduate school editor of LSU Digital Commons. For more information, please [email protected]. THE DETERMINANTS OF NUCLEOSOME PATTERNS AND THE IMPACT OF PHOSPHATE STARVATION ON NUCLEOSOME PATTERNS AND GENE EXPRESSION IN RICE A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Department of Biological Sciences by Qi Zhang B.S., Sichuan Agricultural University, 2012 May 2018 ACKNOWLEDGEMENTS The journey towards the completion of the degree hasn’t been easy, and I would like to express my heartfelt gratitude to everyone who has been around me, listening to me, and supporting me.
    [Show full text]
  • Machine Learning & Learning Machines
    A Brief History • Bootstrapping • Bagging • Boosting (Schapire 1989) • Adaboost (Schapire 1995) What’s So Good About Adaboost • Improves classification accuracy • Can be used with many different classifiers • Commonly used in many areas • Simple to implement • Not prone to over-fitting Ex. “How May I Help You?” easy to find “rules of thumb” that are “often” correct • e.g.: “IF ‘card’ occurs in utterance THEN predict ‘Calling Card’ ” • hard to find single highly accurate prediction rule Bootstrap Estimation • Repeatedly draw n samples from D • For each set of samples, estimate a statistic • The bootstrap estimate is the mean of the individual estimates • Used to estimate a statistic (parameter) and its variance Bagging - Aggregate Bootstrapping • For i = 1 .. M – Draw n*<n samples from D with replacement – Learn classifier Ci • Final classifier is a vote of C1 .. CM • Increases classifier stability/reduces variance Boosting (Schapire 1989) • Randomly select n1 < n samples from D without replacement to obtain D1 – Train weak learner C1 • Select n2 < n samples from D with half of the samples misclassified by C1 to obtain D2 – Train weak learner C2 • Select all samples from D that C1 and C2 disagree on – Train weak learner C3 • Final classifier is vote of weak learners Adaboost - Adaptive Boosting • Instead of sampling, re-weight – Previous weak learner has only 50% accuracy over new distribution • Can be used to learn weak classifiers • Final classification based on weighted vote of weak classifiers Adaboost Terms • Learner = Hypothesis = Classifier • Weak Learner: < 50% error over any distribution • Strong Classifier: thresholded linear combination of weak learner outputs Basic idea of boosting technique In general Boosting Bagging Single Tree.
    [Show full text]
  • Learning Chroma\N States from Chip-‐Seq Data
    Learning Chroman States from ChIP-seq data Luca Pinello GC Yuan Lab Outline • Chroman structure, histone modificaons and combinatorial paerns • How to segment the genome in chroman states • How to use ChromHMM step by step • Further references 2 Epigene-cs and chroman structure • All (almost) the cells of our body share the same genome but have very different gene expression programs…. 3 h?p://jpkc.scu.edu.cn/ywwy/zbsw(E)/edetail12.htm The code over the code • The chroman structure and the accessibility are mainly controlled by: 1. Nucleosome posioning, 2. DNA methylaon, 3. Histone modificaons. 4 Histone Modificaons Specific histone modificaons or combinaons of modificaons confer unique biological func-ons to the region of the genome associated with them: • H3K4me3: promoters, gene acva.on • H3K27me3: promoters, poised enhancers, gene silencing • H2AZ: promoters • H3K4me1: enhancers • H3K36me3: transcribed regions • H3K9me3: gene silencing • H3k27ac: acve enhancers 5 Examples of *-Seq Measuring the genome genome fragmentation assembler DNA DNA reads “genome” ChIP-seq to measure histone data fragments Measuring the regulome (e.g., protein-binding of the genome) Chromatin Immunopreciptation genomic (ChIP) + intervals fragmentation Protein - peak caller bound by DNA bound DNA reads proteins REVIEWS fragments a also informative, as this ratio corresponds to the fraction ChIP–chip of nucleosomes with the particular modification at that location, averaged over all the cells assayed. One of the difficulties in conducting a ChIP–seq con- trol experiment is the large amount of sequencing that ChIP–seq may be necessary. For input DNA and bulk nucleosomes, many of the sequenced tags are spread evenly across the genome.
    [Show full text]
  • Snipa Snpcard
    SNiPAcard Block annotations Block info genomic range chr6:169,562,593-169,598,082 block size 35,490 bp variant count 24 variants Basic features Conservation/deleteriousness Linked genes phyloP μ = -0.515 [-2.942 – gene(s) hit or close-by RP11-417E7.1 , RP11-417E7.2 0.962] phastCons μ = 0.035 [0 – 0.384] eQTL gene(s) RP11-417E7.2 μ = -0.601 [-3.58 – 1.47] potentially regulated RP1-137D17.1 , RP11-417E7.1 , RP3-495K2.3 , THBS2 , WDR27 , XXyac- GERP++ gene(s) YX65C7_A.2 CADD μ = 2.299 [0.198 – 6.035] THBS2 disease gene(s) score Trait annotations Disease gene annotation gene trait source DB source entry/link THBS2 INTERVERTEBRAL DISC DISEASE OMIM MIM:603932 Direct effect on regulation cis-eQTL gene transcript probe tissue min(statistic) (type) source variant(s) RP11-417E7.2 ? ENSG00000261039 aorta 9.21×10-6 (p-value) GTEx Portal V6 2 Putative effect on regulation ENCODE promoter-associated distal DHS (Enhancer) SNiPA enhancer id variant(s) associated SNiPA promoter id associated gene(s) ENCE00000470093 1 ENCP00000050698 RP1-137D17.1 ENCP00000050686 RP3-495K2.3 ENCP00000050695 THBS2 ENCE00000470094 1 ENCP00000050689 RP11-417E7.1 ENCP00000050698 RP1-137D17.1 ENCP00000050686 RP3-495K2.3 ENCP00000050695 THBS2 ENCE00000470180 1 ENCP00000050690 XXyac-YX65C7_A.2 ENCE00000470279 1 ENCP00000050700 WDR27 Regulatory feature cluster element id variant(s) tissue/cell factors ENSR00001233874 1 NHLF H3K36me3, H3K27ac, DNase1 (promoter flanking region) embryonic stem cell (H1ESC) DNase1 HSMMtube H2AZ, H3K4me2, DNase1 Osteobl H3K27ac, H3K36me3 blood (K562)
    [Show full text]
  • And BBAP- Mediated DNA Repair to Overcome Chemoresistance in EZH2 Gain-Of-Function Mutant Diffuse Large B-Cell Lymphoma
    www.impactjournals.com/oncotarget/ Oncotarget, Vol. 6, No.7 HDAC1,2 inhibition impairs EZH2- and BBAP- mediated DNA repair to overcome chemoresistance in EZH2 gain-of-function mutant diffuse large B-cell lymphoma Danielle P. Johnson2,*, Gabriella S. Spitz1,*, Shweta Tharkar1, Steven N. Quayle3, Jeffrey R. Shearstone3, Simon Jones3, Maria E. McDowell1, Hannah Wellman1, Jessica K. Tyler4, Bradley R. Cairns2, Mahesh B. Chandrasekharan1 and Srividya Bhaskara1,2 1 Department of Radiation Oncology, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 2 Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 3 Acetylon Pharmaceuticals, Inc., Boston, MA 4 Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Houston, TX * These authors contributed equally to the study Correspondence to: Srividya Bhaskara, email: [email protected] Keywords: epigenetics, DNA repair, HDAC1,2, chromatin, EZH2 Received: November 01, 2014 Accepted: December 28, 2014 Published: December 31, 2014 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACT Gain-of-function mutations in the catalytic site of EZH2 (Enhancer of Zeste Homologue 2), is observed in about 22% of diffuse large B-cell lymphoma (DLBCL) cases. Here we show that selective inhibition of histone deacetylase 1,2 (HDAC1,2) activity using a small molecule inhibitor causes cytotoxic or cytostatic effects in EZH2 gain-of-function mutant (EZH2GOF) DLBCL cells. Our results show that blocking the activity of HDAC1,2 increases global H3K27ac without causing a concomitant global decrease in H3K27me3 levels.
    [Show full text]
  • A Unified Encyclopedia of Human Functional DNA Elements
    bioRxiv preprint doi: https://doi.org/10.1101/086025; this version posted July 1, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. A unified encyclopedia of human functional DNA elements through fully automated annotation of 164 human cell types Maxwell W. Libbrecht∗ Department of Computer Science and Engineering University of Washington Oscar Rodriguez∗ Department of Genetics and Genomic Sciences Icahn School of Medicine at Mount Sinai Zhiping Weng Program in Bioinformatics and Integrative Biology University of Massachusetts Medical School Jeffrey A. Bilmes Michael M. Hoffman Department of Electrical Engineering Princess Margaret Cancer Centre University of Washington Department of Medical Biophysics Department of Computer Science University of Toronto William S. Noble Department of Genome Sciences Department of Computer Science and Engineering University of Washington July 1, 2019 Abstract Semi-automated genome annotation methods such as Segway enable understanding of chromatin activity. Here we present chromatin state annotations of 164 human cell types using 1,615 genomics data sets. To produce these annotations, we developed a fully-automated annotation strategy in which we train separate unsupervised annotation models on each cell type and use a machine learning classifier to automate the state interpretation step. Using these annotations, we developed a measure of the importance of each genomic position called the \conservation-associated activity score," which we use to aggregate information across cell types into a multi-cell type view.
    [Show full text]
  • What Is Epigenetics? Two Views in Embryology
    What is Epigenetics? Two views in embryology Preformationism (17-19th century): each cell contains preformed elements that enlarge during development. Epigenesis (19th century -): chemical Humunculus reactions among soluble components in Hartsoecker 1695 the cell that execute a complex developmental plan. Chromosomes are Necessary for Development Before the 20th century Walter Sutton, 1902 Theodor Boveri, 1903 Columbia University University of Würzburg USA Germany • Determined that all chromosomes had to be present for proper embryonic development. • Development encoded by irreversible changes in chromosomes? Cell Specialization is Reversible Late 20th and early 21st centuries Skin cell nuclear transfers 99 Central Question: How can a single Original explant fertilized egg give rise to a complex removed Adult frog of \-nu strain Outgrowth of organism with cells of varied as nuclear donor epidermal cells phenotypes? Parent of 1st transfer Donor cells for recipient eggs Enucleation of nuclear transfer recipient eggs 1st nuclear transfer Cells trypsinized Foot web outgrowth and washed prove frog was 2-nu Uncleaved Completely cleaved (70 V) Martially cleaved /c o/\ • Gurdon, Laskey & Reeves 1975 (25%) demonstrated that “cell Dissociated cells for specialization does not involve serial transfer I *^i/ KJpZ* Parent of serial ttransfei r any loss, irreversible activation or Enucleation of 1 recipient eggs recipient eggs Serial nuclear transfer permanent change chromosomal Foot web outgrowth prove frog was 2-nu genes required for development” Uncleaved Completely cleaved (40/O Partially cleaved (30/0 (30%) Nuclear transplant tadpole: l-nu diploid from nucleolus and chromosome counts (present in 36% of serial clones) Fig. 2. Plan of serial nuclear transfer experiments, using nuclei from adult skin celJs.
    [Show full text]
  • Covering 90% of Epigenetic Targets and Transcription Factors
    Epigenetics antibodies Covering 90% of epigenetic targets and transcription factors H2B Modifi cation Writers Epigenetics H2BK5ac p300, ATF2 Epigenetics is the study of heritable changes in gene expression that modify DNA, H2BK12ac p300, CBP, ATF2 RNA, and protein but do not alter the nucleotide sequence. Posttranslational H2A.X H2BS14ph MST1 H2BK15ac p300, CBP, ATF2 Writers, readers, and erasers modifi cations (PTMs) are among the most important types of epigenetic states Modifi cation Writers Readers Erasers H2A.XS139ph ATM, ATR, MDC1, MDC2, PP4 H2BK20ac p300 Epigenetic regulation is a dynamic process and includes writers, readers, and that apply to proteins. PTMs are marks that provide an extensive regulatory DNA-PK NBS1, 53BP1, H2BS33ph TAF1 erasers. Writers place a PTM mark on a specifi c amino acid on histones or other mechanism for cells to signal which genes to turn on and off. Many types H1 BRCA1 H2BS36ph AMPK Modifi cation Writers Readers H2A.XT142ph WSTF APBB1 EYA1/3 proteins. These include histone acetyltransferases (HATs), histone methyltransferases and families of proteins are subject to PTMs, but one of the most highly H2BK120ub UBE2E1, RNF20, RNF40, H1K26me EZH2 L3MBTL1 UBE2A, UBE2B (HMTs), protein arginine methyltransferases (PRMTs), and kinases. Readers bind to decorated is the histone family of proteins. Some examples of PTMs are H1S27ph the epigenetic marks and include proteins with bromodomains, chromodomains, methylation, acetylation, phosphorylation, and ubiquitination. and tudor domains. Epigenetic erasers remove such marks and include histone deacetylases (HDACs), lysine demethylases (KDMs), and phosphatases. The writing, It is essential to use an antibody specifi c to an individual histone modifi cation reading, and erasing of these posttranslational marks lead to changes in chromatin because each one represents a unique signal for gene expression.
    [Show full text]
  • Comprehensive Benchmarking Reveals H2BK20 Acetylation As a Distinctive Signature of Cell-State-Specific Enhancers and Promoters
    Downloaded from genome.cshlp.org on September 27, 2021 - Published by Cold Spring Harbor Laboratory Press 1 Title: Comprehensive benchmarking reveals H2BK20 acetylation as a distinctive signature of cell-state-specific enhancers and promoters. Vibhor Kumar1*, Nirmala Arul Rayan1*, Masafumi Muratani2,3*, Stefan Lim1, Bavani Elanggovan1, XIN Lixia1, Tess Lu1, Harshyaa Makhija1, Jeremie Poschmann1, Thomas Lufkin3,4, Huck Hui Ng3,5# & Shyam Prabhakar1# 1. Computational and Systems Biology, Genome Institute of Singapore, Singapore. 2. Department of Genome Biology, Faculty of Medicine, University of Tsukuba, Japan. 3. Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore. 4. Department of Biology, Clarkson University, Potsdam, New York, USA. 5. Dept of Biological Sciences, National University of Singapore, Singapore. * These authors contributed equally to this work. # Correspondence may be addressed to: Genome Institute of Singapore 60 Biopolis Street #02-01, Genome Building Singapore 138672 Emails: [email protected], [email protected] Running Title: H2BK20ac marks dynamic enhancers and promoters Keywords: H2BK20ac, cell type specific, enhancers, promoters Downloaded from genome.cshlp.org on September 27, 2021 - Published by Cold Spring Harbor Laboratory Press 2 ABSTRACT Although over 35 different histone acetylation marks have been described, the overwhelming majority of regulatory genomics studies focus exclusively on H3K27ac and H3K9ac. In order to identify novel epigenomic traits of regulatory elements, we constructed a benchmark set of validated enhancers by performing 140 enhancer assays in human T cells. We tested 40 chromatin signatures on this unbiased enhancer set and identified H2BK20ac, a little-studied histone modification, as the most predictive mark of active enhancers.
    [Show full text]
  • Tracking Combinatorial Chromatin State Dynamics in Space and Time
    bioRxiv preprint doi: https://doi.org/10.1101/038612; this version posted August 18, 2016. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Taudt et al. METHOD chromstaR: Tracking combinatorial chromatin state dynamics in space and time Aaron Taudt1, Minh Anh Nguyen4, Matthias Heinig3, Frank Johannes2 and Maria Colom´e-Tatch´e1,3* Abstract Background: Post-translational modifications of histone residue tails are an important component of genome regulation. It is becoming increasingly clear that the combinatorial presence and absence of various modifications define discrete chromatin states which determine the functional properties of a locus. An emerging experimental goal is to track changes in chromatin state maps across different conditions, such as experimental treatments, cell-types or developmental time points. Results: Here we present chromstaR, an algorithm for the computational inference of combinatorial chromatin state dynamics across an arbitrary number of conditions. ChromstaR uses a multivariate Hidden Markov Model to determine the number of discrete combinatorial chromatin states using multiple ChIP-seq experiments as input and assigns every genomic region to a state based on the presence/absence of each modification in every condition. We demonstrate the advantages of chromstaR in the context of three common experimental data scenarios. First, we study how different histone modifications combine to form combinatorial chromatin states in a single tissue. Second, we infer genome-wide patterns of combinatorial state differences between two cell types or conditions. Finally, we study the dynamics of combinatorial chromatin states during tissue differentiation involving up to six differentiation points.
    [Show full text]