1 Author’s pre-print manuscript of the following article 2 3 Astilbe thunbergii reduces postprandial hyperglycemia in a type 2 diabetes rat 4 model via pancreatic alpha-amylase inhibition by highly condensed 5 procyanidins. 6 7 Bioscience, Biotechnology, and Biochemistry, 2017, accepted. 8 Check the published version from the bellow link. 9 10 Taylers & Francis Online 11 DOI:10.1080/09168451.2017.1353403 12 http://www.tandfonline.com/doi/full/10.1080/09168451.2017.1353403 13 14 15 1 16 E. Kato et al. 17 Anti-diabetic effect of Astilbe thunbergii 18 19 Research Article 20 Astilbe thunbergii reduces postprandial hyperglycemia in a type 2 diabetes 21 rat model via pancreatic alpha-amylase inhibition by highly condensed 22 procyanidins. 23 Eisuke Kato1*, Natsuka Kushibiki1, Yosuke Inagaki2, Mihoko Kurokawa2, Jun 24 Kawabata1 25 1Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School 26 of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-8589, Japan 27 2Q'sai Co., Ltd., Kusagae, Fukuoka, Fukuoka 810-8606, Japan 28 29 *Corresponding author 30 Tel/Fax: +81 11 706 2496; 31 E-mail address:
[email protected] (E. Kato) 32 2 33 Abstract 34 Type 2 diabetes mellitus (T2DM) is a common global health problem. Prevention 35 of this disease is an important task, and functional food supplements are 36 considered an effective method. Astilbe thunbergii extract (AT) was found to be a 37 potent pancreatic α-amylase inhibitor. AT treatment in a T2DM rat model 38 reduced post-starch administration blood glucose levels. Activity-guided 39 isolation revealed procyanidin as the active component with IC50 = 1.7 µg/mL 40 against porcine pancreatic α-amylase.