DOCSLIB.ORG

Triglyceride Profiling in Adipose Tissues from Obese Insulin Sensitive, Insulin Resistant and Type 2 Diabetes Mellitus Individua

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Triglyceride Profiling in Adipose Tissues from Obese Insulin Sensitive, Insulin Resistant and Type 2 Diabetes Mellitus Individua Al‑Sulaiti et al. J Transl Med (2018) 16:175 https://doi.org/10.1186/s12967-018-1548-x Journal of Translational Medicine RESEARCH Open Access Triglyceride profling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individuals Haya Al‑Sulaiti1, Ilhame Diboun2, Sameem Banu1, Mohamed Al‑Emadi3, Parvaneh Amani3, Thomas M. Harvey1, Alex S. Dömling4, Aishah Latif1 and Mohamed A. Elrayess1,5* Abstract Background: Lipid intermediates produced during triacylglycerols (TAGs) synthesis and lipolysis in adipocytes inter‑ fere with the intracellular insulin signaling pathway and development of insulin resistance. This study aims to compare TAG species and their fatty acid composition in adipose tissues from insulin sensitive (IS), insulin resistant (IR) and type 2 diabetes mellitus (T2DM) obese individuals. Methods: Human subcutaneous and omental adipose tissue biopsies were obtained from 64 clinically characterized obese individuals during weight reduction surgery. TAGs were extracted from the adipose tissues using the Bligh and Dyer method, then were subjected to non-aqueous reverse phase ultra-high performance liquid chromatography and full scan mass spectrometry acquisition and data dependent MS/MS on LTQ dual cell linear ion trap. TAGs and their fatty acid contents were identifed and compared between IS, IR and T2DM individuals and their levels were cor‑ related with metabolic traits of participants and the adipogenic potential of preadipocyte cultures established from their adipose tissues. Results: Data revealed 76 unique TAG species in adipose tissues identifed based on their exact mass. Analysis of TAG levels revealed a number of TAGs that were signifcantly altered with disease progression including C46:4, C48:5, C48:4, C38:1, C50:3, C40:2, C56:3, C56:4, C56:7 and C58:7. Enrichment analysis revealed C12:0 fatty acid to be associated with TAGs least abundant in T2DM whereas C18:3 was found in both depleted and enriched TAGs in T2DM. Signifcant cor‑ relations of various adipose tissue-derived TAG species and metabolic traits were observed, including age and body mass index, systemic total cholesterol, TAGs, and interleukin-6 in addition to adipogenic potential of preadipocytes derived from the same adipose tissues. Conclusion: Pilot data suggest that adipose tissues from obese IR and T2DM individuals exhibit TAG-specifc signa‑ tures that may contribute to their increased risk compared to their IS counterparts. Future experiments are warranted to investigate the functional relevance of these specifc lipidomic profles. Keywords: Lipidomics, Adipose tissue, Triaclyglycerols, Insulin sensitivity, Insulin resistance, Type 2 diabetes mellitus Background of the adipose tissue, leading to progression of insu- Adipose tissue is the main site for storing and mobiliz- lin resistance and type 2 diabetes mellitus (T2DM) [1]. ing energy in response to metabolic demand. Obesity is However, a subset of obese individuals, known as the associated with changes in the structure and function insulin sensitive (IS) obese, maintain insulin sensitivity and exhibit better adipose tissue functions compared to equally obese insulin resistant (IR) counterparts [2]. Obe- *Correspondence: [email protected] 1 Toxicology and Multipurpose Lab, Anti Doping Laboratory Qatar, Sports sity triggers hypertrophy of adipocytes within the subcu- City, Doha, Qatar taneous (SC) adipose tissues to enable accumulation of Full list of author information is available at the end of the article excess triacylglycerols (TAGs). Additional energy intake © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat​iveco​mmons​.org/licen​ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creat​iveco​mmons​.org/ publi​cdoma​in/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Al‑Sulaiti et al. J Transl Med (2018) 16:175 Page 2 of 13 causes further fat accumulation within the omental (OM) Other chemicals and reagents were from Sigma (Munich, depot, which is associated with ectopic fat deposition in Germany). the liver, skeletal muscle and heart tissues [3]. Te subse- quent hyperinsulinemia inhibits hormone sensitive lipase Cohort and triggers the lipoprotein lipase causing additional glu- Participants’ recruitment criteria were described previ- cose intolerance, hyperinsulinemia, hypertriglyceridemia ously [12]. Briefy, 64 consented obese individuals under- and higher risk of insulin resistance in these tissues [4]. going bariatric surgery at AlEmadi hosptial (Doha, Qatar) Analysis of complex biological systems has become were recruited. Protocols were approved by Institutional possible by the newly emerging metabolomics tech- Review Board of ADLQ (X2017000224). Blood was taken niques where metabolites serve as direct indicators of prior to operation and 1–5 g of abdominal SC and OM biochemical activity of complex phenotypes such as insu- adipose tissues biopsies were collected during the surgery lin resistance and T2DM [5]. In this context, lipidomics and stored at − 80 °C until use. Plasma cholesterol, fast- studies were utilized to study diferences between SC and ing glucose and liver function enzymes were measured by OM depots. Tese studies have revealed depot-specifc COBAS INTEGRA (Roche Diagnostics, Basil). IL-6, leptin enrichment of specifc TAGs, glycerophospholipids, and and insulin were determined using commercially available sphingolipids and diferences in the association of lipid ELISA. Insulin resistance was computed by homeostatic species with body mass index, infammation and insulin model assessment (HOMA-IR) [13] using 30th percentile sensitivity [6, 7]. Although TAGs themselves are unlikely (HOMAIR = 2.4) as a threshold point. Accordingly, sub- to be signaling molecules, an increasing body of evidence jects were dichotomized into IS (HOMA-IR < 2.4, n = 18, suggests that lipid intermediates produced during TAG 3 males and 15 females) and IR (HOMA-IR > 2.4, n = 35, 9 synthesis or breakdown interfere with the intracellular males and 26 females). Eleven participants were clinically insulin signaling pathway and contribute to the develop- diagnosed with T2DM (8 males and 3 females). ment of insulin resistance, including free fatty acids, dia- cylglycerols and ceramides [8]. Indeed, elevated fatty acid Preadipocyte culture and diferentiation efux from the adipose tissue stimulates TAG synthesis Stromal vascular fraction (SVF) cells were obtained by col- in the liver and triggers stress of endoplasmic reticulum lagenase digestion of adipose tissues as described previously and stimulation of June kinase pathway in the adipose tis- [12]. Cell pellets were re-suspended in stromal medium sues [9, 10]. Tis leads to an overload of TAG’s synthetic containing Dulbecco’s modifed Eagle’s Medium-F12 capacity, causing an increase in both diacylglycerols (DMEM-F12) supplemented with 10% fetal bovine serum (DAGs) and ceramide levels and further development of (FBS) and Penicillin/Streptomycin, then maintained at insulin resistance in adipocytes [11]. 37 °C with 5% CO2 until confuence. To induce diferentia- Despite various studies investigating lipidomic difer- tion, early passaged stromal vascular fraction (SVF)-derived 4 2 ences in human serum and adipose tissues in relation to preadipocytes (passages 1–3) were grown at 2 × 10 /cm insulin sensitivity, no studies have compared diferences in stromal medium overnight, then incubated in diferen- in TAG signatures and their fatty acid composition in tiation medium (DMEM-F12, 3% FBS, 33 μM biotin, 17 μM adipose tissues from IS, IR and T2DM obese individu- d-pantothenate, 1 μM dexamethasone, 250 μM of methyl- als and their correlations with mediators of metabolic isobutylxanthine, 0.1 μM human insulin, 5 μM of Peroxi- disease. Identifcation of the fatty acids that are enriched some proliferator-activated receptor gamma PPARγ agonist, or depleted in tissues from insulin resistance and T2DM rosiglitazone) for 7 days, followed by 12 days in maintenance individuals could shed light on their functional role in medium containing the same components as the diferen- disease progression, thus providing potential novel tar- tiation medium but omitting methylisobutylxanthine and gets for therapeutic intervention. Te aims of this study rosiglitazone. Diferentiation potential (adipogenic capacity) were to profle TAG species and measure their levels in was determined as a percentage of lipidtox positive stained two fat depots and to compare their fatty acid composi- cells to total number of stained nuclei (DAPI). tion between IS, IR and T2DM individuals. Sample preparation Methods Human SC and OM adipose tissue specimens from IS, IR Materials and T2DM individuals were extracted using the Bligh and Interleukin 6 (IL-6) and leptin ELISAs were from R&D Dyer Method [14]. Homogenization of tissue was car- systems (Abingdon, UK). Insulin ELISA was from Mer- ried out in the gentle MACS Dissociator (Miltenyi Bio- codia Diagnostics (Uppsala, Sweden). 4′,6-Diamidino- tech, Germany) with one volume of PBS for every gram 2-phenylindole (DAPI), and LipidTOX Green Neutral of tissue. Following tissue homogenization, 1 mL of each Lipid were from
Load more

Recommended publications
  • Effect of Parity on Fatty Acids of Saudi Camels Milk and Colostrum
    International Journal of Research in Agricultural Sciences Volume 4, Issue 6, ISSN (Online): 2348 – 3997 Effect of Parity on Fatty Acids of Saudi Camels Milk and Colostrum Magdy Abdelsalam1,2*, Mohamed Ali1 and Khalid Al-Sobayil1 1Department of Animal Production and Breeding, College of Agriculture and Veterinary Medicine, Qassim University, Al-Qassim 51452, Saudi Arabia. 2Department of Animal Production, Faculty of Agriculture, Alexandria University, El-Shatby, Alexandria 21545, Egypt. Date of publication (dd/mm/yyyy): 29/11/2017 Abstract – Fourteen Saudi she-camels were machine milked locations and different feeding regimes, but there is a scare twice daily and fatty acids of colostrum (1-7 days post partum) on the effect of parity of lactating camels on the fatty acids. and milk (10-150 days post partum) were analyzed. Short Therefore, the objective of this experiment was to study the chain fatty acids were found in small percentage in colostrums changes in the fatty acids profile of colostrums and milk of and milk at different parities without insignificant differences she-camel during the first three parities. and the C4:0 and C6:0 don't appear in the analysis. Colostrums has higher unsaturated fatty acids percentage than that of saturated fatty acids while the opposite was found II. MATERIALS AND METHODS in milk of camels. Myiristic acid (C14:0), palmitic (C16:0), stearic (C18:0) and oleic (C18:1) showed the highest A. Animals and Management percentage in either colostrums or milk of she-camels. Parity The present study was carried out on fourteen Saudi she had significant effect on atherogenicity index (AI) which is camels raised at the experimental Farm, College of considered an important factor associated the healthy quality of camel milk.
    [Show full text]
  • (12) Patent Application Publication (10) Pub. No.: US 2014/0155647 A1 Dubois (43) Pub
    US 2014O155647A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2014/0155647 A1 Dubois (43) Pub. Date: Jun. 5, 2014 (54) METHOD FOR THE SYNTHESIS OF DIACIDS Publication Classification OR DESTERS FROMINATURAL FATTY ACDS AND/ORESTERS (51) Int. Cl. C07C 67/303 (2006.01) (71) Applicant: Arkema France, Colombes (FR) CD7C5L/36 (2006.01) (52) U.S. Cl. (72) Inventor: Jean-Luc Dubois, Millery (FR) CPC ............... C07C 67/303 (2013.01); C07C 51/36 (2013.01) (21) Appl. No.: 13/946,292 USPC ........................................... 560/190; 562/592 (57) ABSTRACT (22) Filed: Jul.19, 2013 Disclosed herein a process for the synthesis of diacids or diesters of general formula ROOC (CH)x-COOR, in O O which in represents an integer between 5 and 14 and R is either Related U.S. Application Data H or an alkyl radical of 1 to 4 carbon atoms, starting from (63) Continuation of application No. 12/664,182, filed on long-chain natural monounsaturated fatty acids or esters Apr. 21, 2010, now abandoned, filed as application No. comprising at least 10 adjacent carbonatoms per molecule, of PCT/FR2008/051038 on Jun. 11, 2008. formula CH (CH)n-CHR—CH2—CH=CH-(CH2)p- COOR, in which R represents Horan alkyl radical compris (30) Foreign Application Priority Data ing from 1 to 4 carbon atoms, R is either H or OH, and n and p, which are identical or different, are indices between 2 and Jun. 13, 2007 (FR) ....................................... O755733 11. US 2014/O 155647 A1 Jun. 5, 2014 METHOD FOR THE SYNTHESIS OF DACDS -continued OR DESTERS FROMINATURAL FATTY ACDS AND/ORESTERS 0001.
    [Show full text]
  • Retention Indices for Frequently Reported Compounds of Plant Essential Oils
    Retention Indices for Frequently Reported Compounds of Plant Essential Oils V. I. Babushok,a) P. J. Linstrom, and I. G. Zenkevichb) National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA (Received 1 August 2011; accepted 27 September 2011; published online 29 November 2011) Gas chromatographic retention indices were evaluated for 505 frequently reported plant essential oil components using a large retention index database. Retention data are presented for three types of commonly used stationary phases: dimethyl silicone (nonpolar), dimethyl sili- cone with 5% phenyl groups (slightly polar), and polyethylene glycol (polar) stationary phases. The evaluations are based on the treatment of multiple measurements with the number of data records ranging from about 5 to 800 per compound. Data analysis was limited to temperature programmed conditions. The data reported include the average and median values of retention index with standard deviations and confidence intervals. VC 2011 by the U.S. Secretary of Commerce on behalf of the United States. All rights reserved. [doi:10.1063/1.3653552] Key words: essential oils; gas chromatography; Kova´ts indices; linear indices; retention indices; identification; flavor; olfaction. CONTENTS 1. Introduction The practical applications of plant essential oils are very 1. Introduction................................ 1 diverse. They are used for the production of food, drugs, per- fumes, aromatherapy, and many other applications.1–4 The 2. Retention Indices ........................... 2 need for identification of essential oil components ranges 3. Retention Data Presentation and Discussion . 2 from product quality control to basic research. The identifi- 4. Summary.................................. 45 cation of unknown compounds remains a complex problem, in spite of great progress made in analytical techniques over 5.
    [Show full text]
  • Sustainable Synthesis of Omega-3 Fatty Acid Ethyl Esters from Monkfish Liver Oil
    Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 2 September 2020 doi:10.20944/preprints202009.0020.v1 Article Sustainable synthesis of omega-3 fatty acid ethyl esters from monkfish liver oil Johanna Aguilera-Oviedo 1,2, Edinson Yara-Varón 1,2, Mercè Torres 2,3, Ramon Canela-Garayoa 1,2,*and Mercè Balcells 1,2 1 Department of Chemistry, University of Lleida, Avda. Alcalde Rovira Roure 191, 25198 Lleida, Spain; [email protected] (J.A.-O.); [email protected] (E.Y.-V.); [email protected] (M.B.) 2 Centre for Biotechnological and Agrofood Developments (Centre DBA), University of Lleida, Avda. Alcalde Rovira Roure 191, 25198 Lleida, Spain; [email protected] 3 Department of Food Technology, University of Lleida, Avda. Alcalde Rovira Roure 191, 25198 Lleida, Spain. * Correspondence: [email protected];Tel.: (+34-973702841) Received: date; Accepted: date; Published: date Abstract: The search for economical and sustainable sources of PUFAs within the framework of the circular economy is encouraged by their proven beneficial effects on health. The extraction of monkfish liver oil (MLO) for the synthesis of omega-3 ethyl esters was performed evaluating two blending systems and four green solvents. Moreover, the potential solubility of the MLO in green solvents was studied using the predictive simulation software COSMO-RS. The production of the ethyl esters was performed by one or two step reactions. Novozym 435, two resting cells (Aspergillus flavus and Rhizopus oryzae) obtained in our laboratory and mix of them were used as biocatalysts in a solvent-free system. The yields for Novozym 435, R.
    [Show full text]
  • Peroxisomal Fatty Acid Beta-Oxidation in Relation to the Accumulation Of
    Peroxisomal fatty acid beta-oxidation in relation to the accumulation of very long chain fatty acids in cultured skin fibroblasts from patients with Zellweger syndrome and other peroxisomal disorders. R J Wanders, … , A W Schram, J M Tager J Clin Invest. 1987;80(6):1778-1783. https://doi.org/10.1172/JCI113271. Research Article The peroxisomal oxidation of the long chain fatty acid palmitate (C16:0) and the very long chain fatty acids lignocerate (C24:0) and cerotate (C26:0) was studied in freshly prepared homogenates of cultured skin fibroblasts from control individuals and patients with peroxisomal disorders. The peroxisomal oxidation of the fatty acids is almost completely dependent on the addition of ATP, coenzyme A (CoA), Mg2+ and NAD+. However, the dependency of the oxidation of palmitate on the concentration of the cofactors differs markedly from that of the oxidation of lignocerate and cerotate. The peroxisomal oxidation of all three fatty acid substrates is markedly deficient in fibroblasts from patients with the Zellweger syndrome, the neonatal form of adrenoleukodystrophy and the infantile form of Refsum disease, in accordance with the deficiency of peroxisomes in these patients. In fibroblasts from patients with X-linked adrenoleukodystrophy the peroxisomal oxidation of lignocerate and cerotate is impaired, but not that of palmitate. Competition experiments indicate that in fibroblasts, as in rat liver, distinct enzyme systems are responsible for the oxidation of palmitate on the one hand and lignocerate and cerotate on the other hand. Fractionation studies indicate that in rat liver activation of cerotate and lignocerate to cerotoyl-CoA and lignoceroyl-CoA, respectively, occurs in two subcellular fractions, the endoplasmic reticulum and the peroxisomes but not in the mitochondria.
    [Show full text]
  • Fatty Acid Diets: Regulation of Gut Microbiota Composition and Obesity and Its Related Metabolic Dysbiosis
    International Journal of Molecular Sciences Review Fatty Acid Diets: Regulation of Gut Microbiota Composition and Obesity and Its Related Metabolic Dysbiosis David Johane Machate 1, Priscila Silva Figueiredo 2 , Gabriela Marcelino 2 , Rita de Cássia Avellaneda Guimarães 2,*, Priscila Aiko Hiane 2 , Danielle Bogo 2, Verônica Assalin Zorgetto Pinheiro 2, Lincoln Carlos Silva de Oliveira 3 and Arnildo Pott 1 1 Graduate Program in Biotechnology and Biodiversity in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, Brazil; [email protected] (D.J.M.); [email protected] (A.P.) 2 Graduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, Brazil; pri.fi[email protected] (P.S.F.); [email protected] (G.M.); [email protected] (P.A.H.); [email protected] (D.B.); [email protected] (V.A.Z.P.) 3 Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, Brazil; [email protected] * Correspondence: [email protected]; Tel.: +55-67-3345-7416 Received: 9 March 2020; Accepted: 27 March 2020; Published: 8 June 2020 Abstract: Long-term high-fat dietary intake plays a crucial role in the composition of gut microbiota in animal models and human subjects, which affect directly short-chain fatty acid (SCFA) production and host health. This review aims to highlight the interplay of fatty acid (FA) intake and gut microbiota composition and its interaction with hosts in health promotion and obesity prevention and its related metabolic dysbiosis.
    [Show full text]
  • Sigma Fatty Acids, Glycerides, Oils and Waxes
    Sigma Fatty Acids, Glycerides, Oils and Waxes Library Listing – 766 spectra This library represents a material-specific subset of the larger Sigma Biochemical Condensed Phase Library relating to relating to fatty acids, glycerides, oils, and waxes found in the Sigma Biochemicals and Reagents catalog. Spectra acquired by Sigma-Aldrich Co. which were examined and processed at Thermo Fisher Scientific. The spectra include compound name, molecular formula, CAS (Chemical Abstract Service) registry number, and Sigma catalog number. Sigma Fatty Acids, Glycerides, Oils and Waxes Index Compound Name Index Compound Name 464 (E)-11-Tetradecenyl acetate 592 1-Monocapryloyl-rac-glycerol 118 (E)-2-Dodecenedioic acid 593 1-Monodecanoyl-rac-glycerol 99 (E)-5-Decenyl acetate 597 1-Monolauroyl-rac-glycerol 115 (E)-7,(Z)-9-Dodecadienyl acetate 599 1-Monolinolenoyl-rac-glycerol 116 (E)-8,(E)-10-Dodecadienyl acetate 600 1-Monolinoleoyl-rac-glycerol 4 (E)-Aconitic acid 601 1-Monomyristoyl-rac-glycerol 495 (E)-Vaccenic acid 598 1-Monooleoyl-rac-glycerol 497 (E)-Vaccenic acid methyl ester 602 1-Monopalmitoleoyl-rac-glycerol 98 (R)-(+)-2-Chloropropionic acid methyl 603 1-Monopalmitoyl-rac-glycerol ester 604 1-Monostearoyl-rac-glycerol; 1- 139 (Z)-11-Eicosenoic anhydride Glyceryl monosterate 180 (Z)-11-Hexadecenyl acetate 589 1-O-Hexadecyl-2,3-dipalmitoyl-rac- 463 (Z)-11-Tetradecenyl acetate glycerol 181 (Z)-3-Hexenyl acetate 588 1-O-Hexadecyl-rac-glycerol 350 (Z)-3-Nonenyl acetate 590 1-O-Hexadecyl-rac-glycerol 100 (Z)-5-Decenyl acetate 591 1-O-Hexadecyl-sn-glycerol
    [Show full text]
  • Modeling the Effect of Heat Treatment on Fatty Acid Composition in Home-Made Olive Oil Preparations
    Open Life Sciences 2020; 15: 606–618 Research Article Dani Dordevic, Ivan Kushkevych*, Simona Jancikova, Sanja Cavar Zeljkovic, Michal Zdarsky, Lucia Hodulova Modeling the effect of heat treatment on fatty acid composition in home-made olive oil preparations https://doi.org/10.1515/biol-2020-0064 refined olive oil in PUFAs, though a heating temperature received May 09, 2020; accepted May 25, 2020 of 220°C resulted in similar decrease in MUFAs and fi Abstract: The aim of this study was to simulate olive oil PUFAs, in both extra virgin and re ned olive oil samples. ff fi use and to monitor changes in the profile of fatty acids in The study showed di erences in fatty acid pro les that home-made preparations using olive oil, which involve can occur during the culinary heating of olive oil. repeated heat treatment cycles. The material used in the Furthermore, the study indicated that culinary heating experiment consisted of extra virgin and refined olive oil of extra virgin olive oil produced results similar to those fi samples. Fatty acid profiles of olive oil samples were of the re ned olive oil heating at a lower temperature monitored after each heating cycle (10 min). The out- below 180°C. comes showed that cycles of heat treatment cause Keywords: virgin olive oil, refined olive oil, saturated significant (p < 0.05) differences in the fatty acid profile fatty acids, monounsaturated fatty acids, polyunsatu- of olive oil. A similar trend of differences (p < 0.05) was rated fatty acids, cross-correlation analysis found between fatty acid profiles in extra virgin and refined olive oils.
    [Show full text]
  • Fatty Acid Composition of Oil from Adapted Elite Corn Breeding Materials Francie G
    Food Science and Human Nutrition Publications Food Science and Human Nutrition 9-1995 Fatty Acid Composition of Oil from Adapted Elite Corn Breeding Materials Francie G. Dunlap Iowa State University Pamela J. White Iowa State University, [email protected] Linda M. Pollak United States Department of Agriculture Thomas J. Brumm MBS Incorporated, [email protected] Follow this and additional works at: http://lib.dr.iastate.edu/fshn_hs_pubs Part of the Agronomy and Crop Sciences Commons, Bioresource and Agricultural Engineering Commons, Food Science Commons, and the Nutrition Commons The ompc lete bibliographic information for this item can be found at http://lib.dr.iastate.edu/ fshn_hs_pubs/2. For information on how to cite this item, please visit http://lib.dr.iastate.edu/ howtocite.html. This Article is brought to you for free and open access by the Food Science and Human Nutrition at Iowa State University Digital Repository. It has been accepted for inclusion in Food Science and Human Nutrition Publications by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Fatty Acid Composition of Oil from Adapted Elite Corn Breeding Materials Abstract The fatty acid composition of corn oil can be altered to meet consumer demands for “healthful” fats (i.e., lower saturates and higher monounsaturates). To this end, a survey of 418 corn hybrids and 98 corn inbreds grown in Iowa was done to determine the fatty acid composition of readily-available, adapted, elite corn breeding materials. These materials are those used in commercial hybrid production. Eighty-seven hybrids grown in France (18 of which also were grown in lowa) were analyzed to determine environmental influence on fatty acid content.
    [Show full text]
  • Harvest Season Significantly Influences the Fatty Acid
    biology Article Harvest Season Significantly Influences the Fatty Acid Composition of Bee Pollen Saad N. Al-Kahtani 1 , El-Kazafy A. Taha 2,* , Soha A. Farag 3, Reda A. Taha 4, Ekram A. Abdou 5 and Hatem M Mahfouz 6 1 Arid Land Agriculture Department, College of Agricultural Sciences & Foods, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia; [email protected] 2 Department of Economic Entomology, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt 3 Department of Animal and Poultry Production, Faculty of Agriculture, University of Tanta, Tanta 31527, Egypt; [email protected] 4 Agricultural Research Center, Bee Research Department, Plant Protection Research Institute, Dokki, Giza, Egypt; [email protected] 5 Agricultural Research Center, Plant Protection Research Institute, Dokki, Giza, Egypt; [email protected] 6 Department of Plant Production, Faculty of Environmental Agricultural Sciences, Arish University, Arish 45511, Egypt; [email protected] * Correspondence: elkazafi[email protected] Simple Summary: Harvesting pollen loads collected from a specific botanical origin is a complicated process that takes time and effort. Therefore, we aimed to determine the optimal season for harvesting pollen loads rich in essential fatty acids (EFAs) and unsaturated fatty acids (UFAs) from the Al- Ahsa Oasis in eastern Saudi Arabia. Pollen loads were collected throughout one year, and the Citation: Al-Kahtani, S.N.; tested samples were selected during the top collecting period in each season. Lipids and fatty acid Taha, E.-K.A.; Farag, S.A.; Taha, R.A.; composition were determined. The highest values of lipids concentration, linolenic acid (C ), Abdou, E.A.; Mahfouz, H.M Harvest 18:3 Season Significantly Influences the stearic acid (C18:0), linoleic acid (C18:2), arachidic acid (C20:0) concentrations, and EFAs were obtained Fatty Acid Composition of Bee Pollen.
    [Show full text]
  • The Occurrence of Very Long-Chain Fatty Acids in Oils from Wild Plant Species Originated from Kivu, Democratic Republic of the Congo
    JOURNAL OF ADVANCEMENT IN MEDICAL AND LIFE SCIENCES Journal homepage: http://scienceq.org/Journals/JALS.php Research Article Open Access The Occurrence of Very Long-Chain fatty acids in oils from Wild Plant species Originated from Kivu, Democratic Republic of the Congo M. Kazadi1, P.T. Mpiana2*, M.T. Bokota3, KN Ngbolua2, S. Baswira4 and P. Van Damme5 1Dapartement de Biologie, Centre de Recherches en Sciences Naturelles, Lwiro, Sud Kivu, D.R.Congo 2 Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, D.R. Congo 3Faculté des Sciences, Université de Kisangani, Kisangani, D.R. Congo 4Department de Chimie, Institut Supérieur Pédagogique de Bukavu, D.R. Congo 5Department of Plant Production, Tropical & Subtropical Agriculture & Ethno-botany, Gent University, Belgium. *Corresponding author: P.T. Mpiana, Contact no: +243818116019, E-mail: [email protected] Received: September 8, 2014, Accepted: October 28, 2014, Published: October 29, 2014. ABSTRACT Fatty acids C20-C26 are important for use in oleo-chemical industry whereas they also allow assessing chemotaxonomic relationships among plant taxa. There are however, comparatively few common vegetable fats which contain them in appreciable amounts.Using gas chromatography this type of very long-chain fatty acids was analyzed in oils from Pentaclethra macrophylla (Fabaceae), Millettia dura (Fabaceae), Tephrosia vogelii (Fabaceae),Cardiospermum halicacabum (Sapindaceae), Maesopsis eminii (Rhamnaceae), Podocarpus usambarensis (Podocarpaceae) and Myrianthus arboreus and M. holstii (Moraceae),wild plant species from Kahuzi-Biega National Park and adjacent areas in D.R. Congo. These plants are used by the local population mainly for nutrition and medical purposes.The percentage of very-long chain fatty acids in the analyzed oils ranged from 1.2 to 21.3%.
    [Show full text]
  • Control of Differentiation of a Mammary Cell Line by Lipids
    Proc. Natl. Acad. Sci. USA Vol. 77, No. 3, pp. 1551-1555, March 1980 Cell Biology Control of differentiation of a mammary cell line by lipids (domes/tumor promoters/fatty acids/lysolecithins) RENATO DULBECCO*, MAURO BOLOGNAt, AND MICHAEL UNGER The Salk Institute, 10010 N. Torrey Pines Road, La Jolla, California 92037 Contributed by Renato Dulbecco, December 17, 1979 ABSTRACT A rat mammary cell line (LA7) undergoes profound effect on cultured cells of various types (13, 14). These spontaneous differentiation into domes due to production of effects include stimulation of growth (15), suppression of several specific inducers by the cells. Some of these inducers may be kinds of differentiation and induction of some other lipids, and we show that lipids regulate this differentiation as (16-24), both inducers and inhibitors. One inhibitor is the tumor pro- kinds of differentiation (25-27). TPA also induces a phenotype moter tetradecanoyl-13 phorbol 12-acetate. The inducers are similar to that of transformed cells (28-30), with a reduced saturated fatty acids of two groups: butyric acid and acids with contact inhibition of growth (31), increased production of chain lengths from C13 to C16, especially myristic acid (C14). plasminogen activator (32-35), increased phospholipid turnover Other inducers are myristoyl and palmitoyl lysolecithins, (36-38), decrease of LETS protein (39), and induction of myristic acid methyl ester, and two cationic detergents with a polyamine synthesis (40). TPA may alter the function of the cell tetradecenyl chain. We propose that the lipids with a C14-CI6 plasma membrane, as its alkyl chain affect differentiation by recognizing specific re- suggested by ability to inhibit the ceptors through their alkyl chains and that the effects obtained binding of epidermal growth factor to its receptors at the cell depend on the head groups.
    [Show full text]