Welcome An International Conference of Biomedical Information Perception &
Microsystems will be hosted in one of the most glamorous cities in China,
Chengdu, in June 13-15 of 2018. This conference aims to provide an international forum for biomedical scientists and engineers worldwide to share the latest advances in the booming fields of bioinformation perception and microsystems for biomedical applications. We believe that this gathering will inspire new efforts to expand the frontiers of research and development in these important fields, and promote multidisciplinary research collaboration across institutions and countries. The Conference is sponsored by Southwest University (Chongqing, China) and the
University of Electronic Science & Technology of China (Chengdu,
China).
Yuejun Kang
Jinhong Guo
Qin Yuan
Congress Chairman
Conference Committee General Chairs Yuejun Kang (Southwest University, China) Jinhong Guo (University of Electronic Science and Technology of China, China) Qin Yuan (University of Electronic Science and Technology of China, China)
Technical Program Chairs Hejun Du (Nanyang Technological University, Singapore) Weihua Li (University of Wollongong, Australia) Guoqing Hu (Institute of Mechanics, CAS, China) Jiashu Sun (National Center for Nanoscience and Technology, China) Yi Zhang (Nanyang Technological University, Singapore) Qingjun Liu (Zhejiang University, Zhejiang, China) Xing Ma (Harbin Institute of Technology, Shenzhen, China) Say Hwa Tan (Griffith University, Australia) Xiwei Huang (Hangzhou Dianzi University, Hangzhou, China) Shiyang Tang (Uiversity of Wollongong, Australia)
Conference Secretary Chengchen Zhang (University of Electronic Science and Technology of China, China)
Hosts
. University of Electronic Science and Technology of China
Southwest University
Undertake
School of Life Science and Technology, University of Electronic Science and Technology of China
Co-Organizers
Institute of Medical Equipment, University of Electronic Science and Technology of China
West China Medicine Technology Transfer Center, Sichuan
Sichuan Association of Thousand Talent Plan Experts
Special Issues
Electrophoresis https://onlinelibrary.wiley.com/page/journal/15222683/homepage/call_for_papers.htm
Micromachines http://www.mdpi.com/journal/micromachines/special_issues/medical_IoT
Sensors http://www.mdpi.com/journal/sensors/special_issues/ICBIPM
Sponsors
Suzhou Research Materials Microtech Co.,Ltd（苏州研材微纳科技有限公司）
Chongqing Mike-William Medical Technology Co., Ltd（重庆迈联医疗科技有限公
司 Organizer
1.Conference Secretary
Chengchen Zhang（张成晨）Tel: 15184390116
2.Conference Coordinators
Main Forum: Bo Liu（刘博）Tel:13880495807
Sub-Forum 1: Miaomiao Chen（陈苗苗）Tel:15882445416
Ya Chen（陈亚） Tel: 15108294698
Sub-Forum 2: Yongli Wan（万永利）Tel:15928962354
Sub-Forum 3: Zhengshui Song（宋水正）Tel:13540769298
3.Accomodation
Shuguang Han（韩曙光）Tel:18200115587
Han Li（李晗）Tel:18280126726
4.Guest Service
Chen Wang（王晨）Tel:13281171213
Jinlong Wang（王金龙）Tel:18280404604
Transportation
A B
D
E
F
C
A : Conference Venue: University of Electronic Science and Technology of China (UESTC), Qingshuihe Campus (No.2006, Xiyuan Ave.West Hi-Tech. Zone, Chengdu, Sichuan, P.R. China 611731) 会议地址：电子科技大学清水河校区（成都市高新西区西源大道2006号）
B : Conference Hotel: Holiday Inn Chengdu High-Tech Center (No.1 Xixin Avenue, West Hi-Tech Development Zone, Chengdu, Sichuan, P.R. China 611731) 会议酒店：成都新希望高新中心假日酒店（成都高新西区西芯大道 1 号附 1 号）
C : Chengdu Shuangliu International Airport/成都双流国际机场 D : Chengdu Railway Station/成都站 E : Chengdudong Railway Station/成都火车东站 F: Chengdunan Railway Station/成都火车南站 ------C->B : By Taxi : It takes around 50 minutes and 60 RMB. By Subway: Subway Line 10-(Transfer:Taipingyuan Station)-Subway Line 3 -(Transfer:Chunxi Road)-Subway Line 2 -(Get off :Baicao Road)-Walk D->B : By Taxi : It takes around 40 minutes and 30 RMB. By Subway: Subway Line 7 Outer Ring-(Transfer:Yipintianxia)-Subway Line 2 -(Get off:Baicao Road)-Walk E->B : By Taxi : It takes around 50 minutes and 80 RMB. By Subway: Subway Line 2- (Get off:Baicao Road)-Walk F->B: By Taxi : It takes around 50 minutes and 60 RMB. By Subway:Subway Line 1-(Transfer:Tianfu Square Station)-Subway Line 2 -(Get off: Baicao Road)-Walk B->A : School Shuttle: June 13th, June 14th, June 15th 8:30 am A->B: School Shuttle: June 13th, June 14th 19:00 pm June 15th 13:00 pm
Campus Guide
Room B , Room C
Furong Restaurant
Room A
Room A: Auditorium, Electronic Science and Technology Research Institute, UESTC, Qingshuihe Campus 会议室A：电子科技大学清水河校区，电科院报告厅
Room B: Room 401, Zonghe Building, UESTC, Qingshuihe Campus 会议室B：电子科技大学清水河校区，综合楼401
Room C: Room 309, Zonghe Building, UESTC, Qingshuihe Campus 会议室C：电子科技大学清水河校区，综合楼309
Detailed Agenda
Day 1, Tuesday, June 12, 2018
14:00-22:00 Arrival & Registration Hotel Lobby
Dinner（Small Hot Pot） Brezz Cafe(1F) Holiday Inn 18:30-21:30 晚餐（自助小火锅） 浅川咖啡（1 楼） Day 2, Wednesday, June 13, 2018 Holiday Inn Parking lot 8:30 School Shuttle（From Hotel to UESTC） Holiday Inn 酒店停车场（一楼大堂外）
Opening Ceremony Host: Jinhong Guo（郭劲宏） 9:00-9:30 Welcome Speech by Conference Organizer Speaker: Hongbin Xu（徐红兵）
Welcome Speech by Conference Chair Speaker: Yuejun Kang (康跃军) Keynote Speeches Main Forum Chair: Dongqing Li（李冬青） Dongqing Li（李冬青） University of Waterloo, Waterloo, Canada 9:30-10:10 Host: Yuejun Kang (康跃军) Electrokinetic Microfluidics, Nanofluidics and Room A: Lab-on-Chip Devices Auditorium, ESTI,
10:10-10:40 Coffee Break & Group Photo UESTC 会议室 A: Jongyoon Han 电子科技大学电科 Massachusetts Institute of Technology, 院报告厅 10:40-11:20 Cambridge, USA Ion Selective Membrane (ISM) Microsystems for Biomedical Applications Host: Jianping Fu (傅剑平) Xingyu Jiang（蒋兴宇） National Center for NanoScience and Technology, 11:20-12:00 University of Chinese Academy of Sciences, Beijing, China Microfluidics for Tomorrow’s Medicine 12:00-14:00 Lunch Furong Restaurant 芙蓉餐厅 UESTC
Jianping Fu（傅剑平） 14:00-14:40 University of Michigan, Ann Arbor, USA Synthetic Human Embryology in a Dish Rong Fan（樊荣） Host: Xingyu Jiang（蒋兴宇） Room A: Yale University, New Haven, USA Auditorium, ESTI, 14:40-15:20 BioMEMS Tools for Single-cell Functional UESTC Omics and the Monitoring of Cancer 会议室 A: Immunotherapy 电子科技大学电科 15:20-15:40 Coffee Break 院报告厅 Zuankai Wang（王钻开） City University of Hong Kong, Hong Kong, 15:40-16:20 Host: Rong Fan（樊荣） China Self-propelled Droplets for Biomedical Applications
Yonggang Wen（文勇刚） Nanyang Technological University, Singapore 16:20-17:00 Performance Optimization for Distributed Machine-Learning Applications at Scale:A Swiss-Army-Knife Approach
17:00-17:40 Poster Presentation & Companies Exhibitions
18:00-19:00 Dinner Furong Restaurant 芙蓉餐厅 UESTC 19:00 School Shuttle（From UESTC to Hotel） Day 3, Thursday, June 14, 2018
Holiday Inn Parking lot 8:30 School Shuttle（From Hotel to UESTC） Holiday Inn 酒店停车场（一楼大堂外）
Keynote Speeches Main Forum Chair: Dongqing Li（李冬青） Yuan-Ting Zhang（张元亭） City University of Hong Kong, Hong Kong, China 9:00-9:40 Health Engineering: Wearable “MINDS” for Room A: the Early Prediction of Cardiovascular Auditorium, ESTI, Diseases Host: Yuejun Kang (康跃军) UESTC Leslie Y. Yeo 会议室 A: RMIT University, Melbourne, Australia 电子科技大学清水 9:40-10:20 Plug-and-Actuate On Demand: Multimodal 河校区电科院报告 Modular Individual Addressability of 厅 Microarray Plates 10:20-10:40 Coffee Break
Nam-Trung Nguyen Griffith University, Brisbane, Australia 10:40-11:20 Host: Zuankai Wang（王钻开） Liquid marble based digital microfluidics: fundamental physics and applications
11:20-12:00 Campus Tour UESTC 12:00-14:00 Lunch Furong Restaurant 芙蓉餐厅
Sub-Forums
Sub-Forum 1 Room A: "Electronic Information &Biomedicine" The Auditorium, ESTI, UESTC Outstanding Talent Forum 会议室 A: “电子信息+生物医学”杰出人才论坛 电子科技大学清水河校区电科院报告厅 14:00-17:30 Sub-Forum 2 Room B ： Room 401, Zonghe Building, UESTC, Biomedical Materials and Micro-nano Devices Qingshuihe Campus Forum 会议室 B：电子科技大学清水河校区, 综合楼 401 生物医用材料与微纳米器件论坛
Sub-Forum 3 Room C ： Room 309, Zonghe Building, UESTC, Biomedical Systems and Artificial Qingshuihe Campus Intelligence(AI) Forum 会议室 C：电子科技大学清水河校区, 综合楼 309 生物医学系统与人工智能论坛
18:00-19:00 Dinner Furong Restaurant 芙蓉餐厅 UESTC 19:00 School Shuttle（From UESTC to Hotel） Day 4, Friday, June 15, 2018 Holiday Inn Parking lot 8:30 School Shuttle（From Hotel to UESTC） Holiday Inn 酒店停车场（一楼大堂外）
Sub-Forum 1 Room A: "Electronic Information &Biomedicine" The Auditorium, ESTI, UESTC Outstanding Talent Forum 会议室 A: “电子信息+生物医学”杰出人才论坛 电子科技大学清水河校区电科院报告厅 Sub-Forum 2 Room B ： Room 401, Zonghe Building, UESTC, Biomedical Materials and Micro-nano Devices 9:00-11:00 Qingshuihe Campus Forum 会议室 B：电子科技大学清水河校区，综合楼 401 生物医用材料与微纳米器件论坛
Sub-Forum 3 Room C ： Room 309, Zonghe Building, UESTC, Biomedical Systems and Artificial Qingshuihe Campus Intelligence(AI) Forum 会议室 C：电子科技大学清水河校区，综合楼 309 生物医学系统与人工智能论坛
11:00-11:20 Break
Room A: Closing Ceremony The Auditorium, ESTI, UESTC 11:20-11:40 & 会议室 A: Awards For Best Posters 电子科技大学清水河校区电科院报告厅
12:00-13:00 Lunch Furong Restaurant 芙蓉餐厅 UESTC
13:00 School Shuttle（From UESTC to Hotel）
13:30 Check-out Front Desk Holiday Inn
2018 International Conference of Biomedical Information Perception & Microsystem Sub-Forum 1 "Electronic Information &Biomedicine" Outstanding Talent Forum “电子信息+生物医学”杰出人才论坛 June 14 -15, 2018 Room A: The Auditorium, ESTI, UESTC (电子科技大学清水河校区电科院报告厅) Day 3, Thursday, June 14, 2018
14:00-14:10 Opening Speech Host: Qin Yuan（袁勤）
14:10-14:20 Welcome Speech by Conference Organizer Speaker: Bin Li（李斌）
Invited Talks
Dezhong Yao（尧德中） 14:20-14:50 University of Electronic Science and Technology of China, Chengdu, China Brain Science and Cloud platform for Brainformatics
Xi Xie（谢曦） 14:50-15:20 Sun Yat-Sen University, Guangzhou, China Host: Yufei Liu（刘玉菲） Nanostraw-device for highly efficient gene transfection
Weiwei Zhao（赵维巍） Harbin Institute of Technology, Shenzhen, China 15:20-15:50 A general approach for flexible and foldable low-resistivity paper/fabric-based electronics
15:50-16:20 Coffee Break & Group Photo
Yufei Liu（刘玉菲） Chongqing University, Chongqing, China 16:20-16:50 Integrated Microsystems for Early Diagnosis and Precision Bio-Medical Application
Xiaochen Dong（董晓臣） 16:50-17:20 Nanjing Tech University, Nanjing, China Host: Weiwei Zhao（赵维巍） NIR Photosensitizers for Targeted Cancer Phototherapy
Xing Ma（马星） 17:20-17:50 Harbin Institute of Technology, Shenzhen, China Micro/Nano-Motors for Biomedical Applications
Day 4, Friday, June 15, 2018
Zhizhao Che（车志钊） Tianjin University, Tianjin, China 9:00-9:30 Heat and mass transfer in simple and compound droplets moving in microchannels Huajin Tang（唐华锦） 9:30-10:00 Sichuan University, Chengdu, China Neuromorphic Computing Approach to Auditory and Visual Perception Host: Yuejun Kang（康跃军） Tuan Guo（郭团） 10:00-10:30 Jinan University, Guangzhou, China Plasmonic fiber-optic biochemical and electrochemical sensing Wei Liu（刘威） 10:30-11:00 Wuhan University, Wuhan, China Nanomaterials based cancer diagnosis and therapy
13 2018 International Conference of Biomedical Information Perception & Microsystem Sub-Forum 2 Biomedical Materials and Micro-nano Devices Forum 生物医用材料与微纳米器件论坛 June 14 -15, 2018 Room B：Room 401, Zonghe Building, UESTC, Qingshuihe Campus（电子科技大学清水河校区综合楼 401) Day 3, Thursday, June 14, 2018
Hejun Du（杜和军） Nanyang Technological University, Singapore 14:00-14:30 Manipulation of Microparticles Into 3D Matrix Patterns Using Standing Surface Acoustic Waves Microfluidics（invited）
Guoqing Hu（胡国庆） Session Chair: Institute of Mechanics, Chinese Academy of Sciences, Beijing, China 14:30-15:00 Fei Duan（段飞） Inertial and Viscoelastic Effects for Micro/nano-particles Manipulation
（invited）
Shi-Yang Tang（唐诗杨） University of Wollongong, Wollongong, Australia 15:00-15:30 Microfluidic Mass Production of Stabilized and Stealthy Liquid Metal Nanoparticles（invited）
15:30-15:50 Coffee Break
Fei Duan（段飞） Nanyang Technological University, Singapore 15:50-16:20 Controlled Wetting and Evaporation Transition in a Drying Sessile Droplet（invited）
Peng Xue（薛鹏） Southwest University, Chongqing, China 16:20-16:40 Surface Modification of Polydimethylsiloxane (PDMS) to Enhance Hemocompatibility for Potential Applications in Medical Implants or Session Chair: Devices Shi-Yang Tang（唐诗杨）
Qianbin Zhao（赵乾斌） University of Wollongong, Wollongong, Australia 16:40-17:00 Inertial microparticle manipulation by sheath flow-enhanced secondary flow in slanted groove microchannel
Ye Tian（田野） 17:00-17:20 The University of Hong Kong, Hong Kong, China Bioinspired Cavity-Microfibers from Microfluidics
Day 4, Friday, June 15, 2018
Yi Zhang（张翼） Nanyang Technological University, Singapore 9:00-9:30 Polydopamine-Modified Magnetic Digital Microfluidic Platform for Biosensing（invited） Session Chair: Jun Zhang（张 俊） Fei Liu（刘飞）
Wenzhou Medical University, Wenzhou, China 9:30-10:00 Isolation and Quantitative Analysis of Extracellular Vesicles for Cancer Early Detection（invited）
14 2018 International Conference of Biomedical Information Perception & Microsystem
Jun Zhang（张俊） Nanjing University of Science and Technology, Nanjing, China 10:00-10:20 A Novel Single-layer Microchannel for Continuous Sheathless Single-stream Particle Inertial Focusing
Xinyi Guo（郭欣仪） Tianjin University, Tianjin, China Session Chair: Yi Zhang（张翼） 10:20-10:40 Cell Membrane Poration towards Intracellular Delivery based on Gigahertz Electromechanical Resonators
Yang Yang（杨洋） Tianjin University, Tianjin, China 10:40-11:00 Selective Trapping and Controllable Release of Single Cell via Three-Dimensional Acoustic Fluidics
15 2018 International Conference of Biomedical Information Perception & Microsystem Sub-Forum 3 Biomedical Systems and Artificial Intelligence(AI) Forum 生物医学系统与人工智能论坛 June 14 -15, 2018 Room C：Room 309, Zonghe Building, UESTC, Qingshuihe Campus（电子科技大学清水河校区综合楼 309） Day 3, Thursday, June 14, 2018
Feng Guo（郭峰） 14:00-14:30 Indiana University, Bloomington, USA Acoustofluidic Manipulation of Single Cells（invited）
Chunxia Zhao（赵春霞）
The University of Queensland, Brisbane, Australia 14:30-15:00 Engineered Micro/nanostructures for Drug Delivery and Controlled Release（invited）
Kan Liu（刘侃） Session Chair: Ling Yu（余玲） University of Electronic Science and Technology of China, Chengdu, China 15:00-15:30 Highly efficient isolation and accurate in situ analysis of circulating tumor cells (CTCs) by a commercially available microfluidic device （invited）
Chuanbiao Wen（温川飙） Chengdu University of TCM, Chengdu, China 15:30-16:00 Research on intelligent algorithm model of TCM syndrome differentiation and treatment based on TCM（invited）
Ling Yu（余玲） Southwest University, Chongqing, China 16:00-16:30 Fast Prototyping and Fabrication of Micro Devices in a Non-cleanroom Setting（invited）
Jin Yang（杨进） Chongqing University, Chongqing, China 16:30-16:50 TENG based Pressure Sensor for Continuous Measurement of Human Arterial Pulse Wave Session Chair: Chunxia Zhao （赵春霞） Huaying Chen（陈华英） Harbin Institute of Technology, Shenzhen, China 16:50-17:10 Distance-based Quantification of Blood Glucose using a Paper-based Microfluidic Device
Zifei Xu（徐梓菲） Nanjing University of Aeronautics & Astronautics, Nanjing, China 17:10-17:30 Development of a Portable Electrical Impedance Tomography System with Red Pitaya STEMlab
Day 4, Friday, June 15, 2018
Lei Xi（奚磊） Southern University of Science and Technology, Shenzhen, China 9:00-9:30 Session Chair: Peng Xue（薛鹏） Miniaturization in photoacoustic microscopy and its applications in microfluidics（invited）
16 2018 International Conference of Biomedical Information Perception & Microsystem
Wenxue Wang（王文学）
Chengdu Tianxia120 Co., Ltd, Chengdu, China（成都天下医森数字科技有
9:30-10:00 限公司） The Application of Intelligent Digital Technology in the Active Health Management（invited）
Hongpeng Zhang（张洪朋） 10:00-10:20 Dalian Maritime University, Dalian, China Design of a high-flux microfluidic fluid oil detection chip
Shuting Pan（潘书婷） Tianjin University, Tianjin, China Session Chair: Wenxue Wang 10:20-10:40 A Label-free Immunosensor based on Gigahertz Resonator Enhanced （王文学） Fibre Optic SPR
Changhao Li（李长昊） University of Electronic Science and Technology of China, Chengdu, China 10:40-11:00 Measurement and Analysis of Arm Muscle Movement with Ultrasound Imaging
17 2018 International Conference of Biomedical Information Perception & Microsystem
18 2018 International Conference of Biomedical Information Perception & Microsystem
A. Main Forum A.01 Dongqing Li:Electrokinetic Microfluidics, Nanofluidics and Lab-on-Chip Devices………………………………2 A.02 Jongyoon Han:Ion Selective Membrane (ISM) Microsystems for Biomedical Applicationss…………………4 A.03 Xingyu Jiang:Microfluidics for Tomorrow’s Medicine………………………………………………………………6 A.04 Jianping Fu:Synthetic human embryology in a dish……………………………………………………………………8 A.05 Rong Fan:BioMEMS tools for single-cell functional omics and the monitoring of cancer immunotherapy …………………………………………………………………………………………………………………………………………10 A.06 Zuankai Wang:Self-propelled droplets for biomedical applications………………………………………………12 A.07 Yonggang Wen:Performance Optimization for Distributed Machine-Learning Applications at Scale: A Sw i ss-Army-Knife Approach ……………………………………………………………………………………………………14 A.08 Yuan-Ting Zhang:Health Engineering: Wearable “MINDS” for the Early Prediction of Cardiovascular D i s e a s e s …………………………………………………………………………………………………………………… …………16 A.09 Leslie Yeo:Plug-and-Actuate On Demand: Multimodal Modular Individual Addressability of Microarray P l a t e s ………………………………………………………………………………………………………………………………… 18 A.10 Nam-Trung Nguyen:Liquid marble based digital microfluidics: fundamental physics and applications…20
B. Sub-Forum 1: "Electronic Information &Biomedicine" Outstanding Talent Forum B.01 Dezhong Yao:Brain Science and Cloud platform for Brainformatics………………………………………………23 B.02 Xi Xie:Nanostraw-device for highly efficient gene transfection……………………………………………………25 B.03 Weiwei Zhao:A general approach for flexible and foldable low-resistivity paper/fabric-based electronics… …………………………………………………………………………………………………………………………………………27 B.04 Yufei Liu:Integrated Microsystems for Early Diagnosis and Precision Bio-Medical Application……………29 B.05 Xiaochen Dong:NIR Photosensitizers for Targeted Cancer Phototherapy………………………………………31 B.06 Ma Xing:Micro/Nano-Motors for Biomedical Applications…………………………………………………………33 B.07 Zhizhao Che:Heat and mass transfer in simple and compound droplets moving in microchannels………35 B.08 Huajin Tang:Title of presentation (Times New Roman, Center, 14 lbs, bold, single spacing, paragraph spacing 0.5 lines before)………………………………………………………………………………………………………………………………………37 B.09 Tuan Guo:Plasmonic fiber-optic biochemical and electrochemical sensing……………………………………39 B.10 Wei Liu:Enhanced cancer immunotherapy by cancer cell membrane camouflaged nanoparticles…………41
C. Biomedical Materials and Micro-nano Devices Forum Meeting C.01 Hejun Du:Manipulation of Microparticles Into 3D Matrix Patterns Using Standing Surface Acoustic Waves Microfluidics……………………………………………………………………………………………………………………………………44 C.02 Guoqing Hu:Inertial and viscoelastic effects for micro/nano-particles manipulation…………………………46 C.03 Shiyang Tang:Microfluidic Mass Production of Stabilized and Stealthy Liquid Metal Nanoparticles………48 C.04 Fei Duan:Controlled Wetting and Evaporation Transition in a Drying Sessile Droplet…………………………50 C.05 Peng Xue:Surface Modification of Polydimethylsiloxane (PDMS) to Enhance Hemocompatibility for Potential Applications in Medical Implants or Devices……………………………………………………………………………………………52 C.06 Qianbin Zhao:Inertial microparticle manipulation by sheath flow-enhanced secondary flow in slanted groove microchannel……………………………………………………………………………………………………………………………………… 54 C.07 Ye TIAN:Bioinspired Cavity-Microfibers from Microfluidics…………………………………………………………56
19 2018 International Conference of Biomedical Information Perception & Microsystem
C.08 Yi Zhang:Polydopamine-Modified Magnetic Digital Microfluidic Platform for Biosensing…………………58 C.09 Fei Liu:Isolation and Quantitative Analysis of Extracellular Vesicles for Cancer Early Detection……………60 C.10 Jun Zhang:A novel single-layer microchannel for continuous sheathless single-stream particle inertial f o c u s i n g …………………………………………………………………………………… ……………………………………………… 62 C.11 Xinyi Guo:Cell membrane poration towards intracellular delivery based on gigahertz electromechanical r e s o n a t o r s …………………………………………………………………………………………………………………………… 64 C.12 Yang Yang:Selective Trapping and Controllable Release of Single Cell via Three-Dimensional Acoustic F l u i d i c s ……………………………………………………………………………………………………………………………… 66
D. Biomedical Systems and Artificial Intelligence(AI) Forum Meeting D.01 Feng Guo:Acoustofluidic Manipulation of Single Cells………………………………………………………………69 D.02 Chun-Xia Zhao:Engineered micro/nanostructures for drug delivery and controlled release………………71 D.03 KanLiu:A microvalves-chip based on PDMS for microfluidics………………………………………………………73 D.04 Chuan-Biao WEN:Research on intelligent algorithm model of TCM syndrome differentiation and treatment based on TCM ……………………………………………………………………………………………………………………… 75 D.05 Yu Ling:“do-it-yourself”: Fast prototyping and fabrication of micro devices in a non-cleanroom setting… …………………………………………………………………………………………………………………………………………77 D.06 Jin Yang:TENG based pressure sensor for continuous measurement of human arterial pulse wave………79 D.07 Huaying Chen:Distance-based quantification of blood glucose using a paper-based microfluidic device… …………………………………………………………………………………………………………………………………………81 D.08 Zifei Xu:Development of a Portable Electrical Impedance Tomography System with Red Pitaya STEMlab… …………………………………………………………………………………………………………………………………………83 D.09 Lei Xi:Miniaturization in photoacoustic microscopy and its applications in microfluidics……………………85 D.10 Wenxue Wang:The application of Intelligent digital technology in the active health management………87 D.11 Hongpeng Zhang:Design of a high-flux microfluidic fluid oil detection chip…………………………………89 D.12 Shuting Pan:A label-free immunosensor based on gigahertz resonator enhanced fibre optic SPR………91 D.13 Li Changhao:Measurement and Analysis of Arm Muscle Movement with Ultrasound Imaging……………93
E. Poster E.01 GPU-based Ultrasound Plane-wave Spatial Compound Imaging………………………………………………………96 E.02 A pleiotropic micro nano-carrier based on Ginsenoside Rg3 encapsulated Fe3O4@SiO2 microbubbles for both drug delivery and molecular imaging …………………………………………………………………………………… 97 E.03 Reflection of the Traceable Mode of Establishing High-quality for the TCM Based on the Traceability Technology of Safety and Interconnection …………………………………………………………………………………………………… 98 E.04 Algorithm Research on Correlation Model between TCM Constitution and Physical Examination Index Based on RBF neural network ………………………………………………………………………………………………………………… 99 E.05 Research on Intelligent Syndrome Differentiation Model of Stomachache in Traditional Chinese Medicine Based on BP Neural Network ……………………………………………………………………………………………………………… 100 E.06 A micro-rod magnetic SERS probe fabrication & application in biomedical detection………………………101 E.07 Application and Development of Artificial Intelligence in TCM Electrical Acupoint Stimulation Equipment… ………………………………………………………………………………………………………………………………………1 0 2 E.08 Feasible Recipes for Cytop Patterning…………………………………………………………………………………103 E.09 Deformation response measurement of cells manipulated by optical tweezers based on laser interference… …………………………………………………………………………………………………………………………………………………………… 104 E.10 Microparticle Separation via Traveling Surface Acoustic Waves (TSAWs)………………………………………105
20 2018 International Conference of Biomedical Information Perception & Microsystem
E.11 Enhanced biofilm distribution and power generation of membraneless microbial fuel cells with a serpentine microchannel…………………………………………………………………………………………………………………………………106 E.12 Integrated treatment and Detection of microalgae cells on a microfluidic chip………………………………107 E.13 Photoacoustofluidics: combining light and sound in microfluidic chip…………………………………………108 E.14 A Bio-inspired Airflow Directional Hair Sensor based on Quaternary Concentric FBGs………………………109 E.15 Precision Positioning System Based on Aerostatic Guide Drived by Ultrasonic Motor………………………110 E.16 Microchannel structure design for cell capture by holographic array optical tweezers………………………111 E.17 Research on Mach-Zehnder Interferometric Sensing Characteristics Based on Seven-core Fiber and Spherical S y m m e t r y ……………………………………………………………………………………………………………………………… 112 E.18 Research on shape sensing fiber optic sensing method of biomimetic flexible antenna……………………113 E.19 Raman probe fiber optimization based on CTO………………………………………………………………………114 E.20 On-chip microparticle and cell washing using co-flow of viscoelastic fluid and Newtonian fluid…………115 E.21 Achievement of nanostructured interfaces via flipping over electroless deposited metal electrodes for highly sensitive electronic skin………………………………………………………………………………………………………………………116 E.22 The tianxia120 『Medical - Drug - Inspection - Insurance』One-stop Digital Active health Follow-up Service P l atform ………………………………………………………………………………………………………………………………………117 E.23 Flexible Antenna Design on PDMS Substrate for Implantable Bioelectronics Applications…………………118 E.24 PEGDA/PVP microneedles with tailorable matrix constitutions for controllable transdermal drug delivery… ………………………………………………………………………………………………………………………………………1 1 9 E.25 Accurate Electrochemical Blood Glucose Monitoring with a Smartphone by Adding Blood Cells Filtration Me mbrane …………………………………………………………………………………………………………………………………… 120 E.26 Numerical Modeling of Impedance-based Lab-in-a-Tube Chip for Biofluidsnalysis Analyses………………121 E.27 The Realization of Pulsed-Wave Doppler Spectrum Estimation Module…………………………………………122 E.28 A Smartphone-Powered Photochemical Dongle Based on Test Strip for Remote Uric Acid Monitoring…123
21 2018 International Conference of Biomedical Information Perception & Microsystem
22 2018 International Conference of Biomedical Information Perception & Microsystem
A. Main Forum
23 2018 International Conference of Biomedical Information Perception & Microsystem A.01 Dongqing Li Professor Department of Mechanical and Mechatronics Engineering
University of Waterloo Waterloo, Canada
Biography Dr. Dongqing Li is a professor at the University of Waterloo, Canada. Dr. Li’s research is in the areas of electrokinetic microfluidics, nanofluidics and lab-on-a-chip technology. Dr. Li founded an international journal—Microfluidics and Nanofluidics, and served as the Editor-in-Chief from 2004 to 2012. Dr. Li was the Editor-in-Chief of the Encyclopedia of Microfluidics and Nanofluidics. Dr. Li was the Canada Research Chair in Microfluidics and Nanofluidics from 2008 to 2013. Dr. Li has published over 300 papers in international journals, more than 100 papers in conference proceedings, 31 book chapters and 3 books. Google Scholar citation number of Dr. Li’s publication is over 18700, and his H-index is 67.
24 2018 International Conference of Biomedical Information Perception & Microsystem Electrokinetic Microfluidics, Nanofluidics and Lab-on-Chip Devices Dongqing Li Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1 *Email: [email protected]
Microfluidics and nanofluidics play key roles in the development of various Lab-on-Chip technologies. Lab-on-a-chip devices are miniaturized bio-medical laboratories on a small glass/plastic plate. These lab chips can duplicate the specialized functions of their room-sized counterparts such as clinical diagnoses and tests. The key microfluidic functions required in various lab-on-a-chip devices include pumping and mixing liquids, controlling bio-reactions, dispensing samples and reagents, and separating molecules and cells/particles. At microscale, various electrokinetic phenomena originate from the electrostatic charges or induced charges at the solid-liquid interfaces and the interactions of these charges with the applied electric field. These electrokinetic phenomena, including electroosmosis, electrophoresis and dielectrophoresis are critical to variety of microfluidic processes and technology. Using electrokinetic microfluidics can realize many fluidic functions and can make the Lab-on-a-chip devices fully automatic, independent of external support (e.g., tubing, valves and pump), and truly portable. Therefore, understanding, modeling and controlling of various electrokinetic microfluidic phenomena and processes are essential to systematic design and operation control of the lab-on-a-chip systems.
This presentation will review the advancement of electrokinetic microfluidics and nanofluidics, including a novel method of fabricating small nanochannels on PDMS chips, separation of nanoparticles by dielectrophoresis, detection of single molecules by nanofluidic-based resistive pulse sensor. Some examples of microfluidic lab-on-a-chip devices such as real-time PCR chip, immunoassay chip and flow cytometer chip will be presented.
25 2018 International Conference of Biomedical Information Perception & Microsystem A.02
Jongyoon Han Professor Department of Electrical Engineering and Computer Science Department of Biological Engineering Massachusetts Institute of Technology Cambridge, USA
Biography Jongyoon Han received B.S.(1992) and M.S.(1994) degree in physics from Seoul National University, Korea, and a Ph.D. degree in applied physics from Cornell University in 2001. He is currently a Professor of Electrical Engineering as well as a Professor of Biological Engineering, Massachusetts Institute of Technology. He received 2009 Analytical Chemistry Young Innovator Award from the American Chemical Society, as well as NSF CAREER Award (2004). He has published more than 140 peer-reviewed journal papers. His research interests cover diverse topics, including molecular sensing, biosample preparation, biomanufacturing, neurotechnology, and desalination. He is also one of the principal investigators in Singapore-MIT Alliance for Research and Technology (SMART) centre, leading the team of researchers in Singapore as well as at MIT. He is also the co-lead PI for MIT’s involvement in NIIMBL (National Institute for Innovation in Manufacturing Biopharmaceuticals), one of the public-private partnership for advancing manufacturing technologies in biopharmaceutical industry.
26 2018 International Conference of Biomedical Information Perception & Microsystem Ion Selective Membrane (ISM) Microsystems for Biomedical Applications Wei Ouyang1, Matthew Flavin1,2, Daniel Freeman2, Yechang Guo3, Wei Wang3, Wei Liu4, Lingyan Gong4, Zirui Li4, Jongyoon Han1* 1Massachusetts Institute of Technology, Cambridge, MA, USA 2Draper Laboratories, Cambridge, MA, USA, 3Peking University, Beijing, China, 4Wenzhou University, Wenzhou, China, *Email: [email protected]
Ion Selective Membranes (ISM) are ion conducting materials with properties of conductive only to positive/negative ions, or a specific ion. Microsystems utilizing ISMs, either via nanofluidic channels or conventional polymer-based membranes, provide unique functionality of measuring, controlling and modulating ion concentrations, with broad class of applications in biomedical engineering. In this talk, I will showcase ISM-enabled microsystems applied in biosensing and neuromodulation. One of the fundamental phenomena related to ISM is Ion Concentration Polarization (ICP), where charge carrier mismatch at the boundaries of ISM leads to local ion concentration modulation under an externally applied current/voltage bias. This phenomenon can be manipulated in microfluidic system to rapidly concentrate the biomolecules, generate low-conductivity zone for more sensitive electrical biosensing. In addition, utilizing ionophores that specifically bind to Ca++, K+, and others, one can create local modulation of a specific ion near nerve fiber, which can then be utilized to stimulate and inhibit the nerve signal communication in a beneficial manner.
References Ouyang W, et al. Enabling electrical biomolecular detection in high ionic concentrations and enhancement of the detection limit thereof by coupling a nanofluidic crystal with reconfigurable ion concentration polarization. Lab Chip 2017, 17:3772-3784. Song YA, et al. Electrochemical Activation and Inhibition of Neuromuscular Systems through Modulation of Ion Concentrations with Ion-Selective Membranes. Nature Mat. 2011, 10:980–986. Li Z, et al. Accurate Multiphysics Numerical Analysis of Particle Preconcentration based on Ion Concentration Polarization. Int. J. Appl. Mechanics 2017, in press.
27 2018 International Conference of Biomedical Information Perception & Microsystem A.03 Xingyu Jiang National Center for NanoScience and Technology, University of Chinese Academy of Sciences, Beijing, 100190 P. R. China Email: [email protected] Web: www.jiangxingyu.com
Biography
Xingyu Jiang is a Professor at the National Center for NanoScience and Technology and the University of the Chinese Academy of Sciences. He obtained his BS at the University of Chicago (1999) and PhD at Harvard University (with Prof. George Whitesides, 2004). He started in the NCNST in 2005 and has remained there since. His research interests include surface chemistry, microfluidics and nanoparticles. He was awarded the “Hundred Talents Plan” of the Chinese Academy of Sciences, the National Science Foundation of China’s Distinguished Young Scholars Award, the Scopus Young Researcher Gold Award, the Human Frontier Science Program Young Instigator Award. He is a Fellow of the Royal Society of Chemistry, an associate editor of Nanoscale (Royal Society of Chemistry, UK). conventional
28 2018 International Conference of Biomedical Information Perception & Microsystem Microfluidics for Tomorrow’s Medicine Xingyu Jiang National Center for NanoScience and Technology, University of Chinese Academy of Sciences,Beijing, 100190 China Email: [email protected]
Keywords: microfabrication, fluidics, nanoparticles, disease
Abstract text (Times New Roman 12 lbs, single spacing, justify). Microfluidics represent a great platform for medicine, especially medicine for the future. We demonstrate that microfluidics can improve the performance of the diagnostics of many types of diseases. These new tools for medicine are driven by miniaturization and the ability to combine it with modifications on solid substrates. Some of our adventure into this direction has the ability to dramatically improve many types of exsiting assays in their analytical performances. Combined with nanoparticles and nanomaterials, microfluidics show great promise in developing novel sensing platforms and realizing point-of-care detection of biomarkers with unprecedented speed. These detection platforms also allow biosensors to be used for screening for therapeutics, e.g., nanocarriers for drug delivery, gene silencing, gene editing and beyond.
Selected references: 1. Wang P, Zhang LM, Zheng WF, Cong LM, Guo ZR, Xie YZY, Wang L, Tang RB, Feng Q, Hamada Y, Gonda K, Hu ZJ, Wu XC, Jiang XY, Angew Chem Int Ed, DOI: 10.1002/anie.201708689. (2017). 2. Yang MZ, Zhang W, Yang JC, Hu BF, Cao FJ, Zheng WS, Chen YP, Jiang XY, Sci Adv, 3(12), eaao4862. (2017). 3. Lei YF, Tang LX, Xie YZY, Xianyu YL, Zhang LM, Wang P, Hamada Y, Jiang K, Zheng WF, Jiang XY, Nat Commun, DOI: 10.1038/ncomms15130. (2017). 4. Chen YP, Xianyu YL, Jiang XY, Accounts Chem Res, 50, 310-319. (2017).
29 2018 International Conference of Biomedical Information Perception & Microsystem A.04 Jianping Fu Associate Professor Department of Mechanical Engineering, Biomedical Engineering, Cell and Developmental Biology University of Michigan, Ann Arbor, USA
Biography
Jianping Fu is an Associate Professor at the University of Michigan, Ann Arbor, with a primary appointment in the Mechanical Engineering Department and courtesy appointments in the Biomedical Engineering Department and the Cell and Developmental Biology Department. He also serves as the Associate Director for the Michigan Center for Integrative Research in Critical Care (MCIRCC) and is a Core Faculty Member for the UM Center for Organogenesis, the UM Comprehensive Cancer Center, and the UM Center for Systems Biology. Dr. Fu’s research interests lie at the nexus of bioengineering, biophysics, and biology. Specifically, his research group integrates micro/nanoengineering, single-cell technologies, and systems and synthetic biology methods with new discoveries of mechanobiology, epigenetics, and stem cell biology for advancing understanding of human development and cancer biology. Dr. Fu is the recipient of the American Heart Association Scientist Development Award (2012), the National Science Foundation CAREER Award (2012), the Mechanical Engineering Outstanding Faculty Achievement Award (2014), the Robert M. Caddell Memorial Award for Research (2014), the Ted Kennedy Family Team Excellence Award (2015), and the Rising Star Award from Biomedical Engineering Society - Cellular and Molecular Bioengineering (2016). Dr. Fu's research group is currently supported by the National Science Foundation, the National Institutes of Health, the American Heart Association, and some other foundations and agencies.
30 2018 International Conference of Biomedical Information Perception & Microsystem Synthetic human embryology in a dish Jianping Fu1, 2, 3 1Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48105, U.S.A. 2Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48105, U.S.A. 3Department of Cell and Developmental Biology, University of Michigan School of Medicine, Ann Arbor, Michigan 48105, U.S.A. *Email: [email protected]
Implantation is a critical developmental milestone for early human embryogenesis and successful pregnancy. During implantation, the pluripotent epiblast gives rise to the squamous amnion and the columnar embryonic disc, which together enclose the amniotic cavity to form an asymmetric cystic structure termed the amniotic sac. The development of the amniotic sac is the keystone for post-implantation human embryogenesis, as the columnar epiblast and the squamous amnion eventually develop into the embryo proper and the enveloping amniotic membrane, respectively, which together constitute the core of a human embryo. Despite its fundamental significance, the development of the amnion and the amniotic sac in humans is poorly understood. Here, we report the first in vitro model for multiple post-implantation human embryogenic events centered around the amniotic sac development, by culturing human pluripotent stem cells (hPSCs) in a bioengineered niche that mimics the mechanical softness and the physical dimensionality of the implantation microenvironment. Specifically, we find that hPSCs can self-organize to form three-dimensional asymmetric cystic structure - herein termed the amniotic sac embryoid (ASE) - that recapitulates the differentiation of amnion, and further, the asymmetric morphogenesis and bipolar amnion-epiblast patterning seen in human amniotic sac development in vivo. Intriguingly, our findings show that biomimetic physical niche cues are both necessary and sufficient for the amniotic induction that is indispensable for the development of the ASE. In addition to the bipolar amnion-epiblast patterning, upon further development, the ASE initiates a process that resembles posterior primitive streak development at early gastrulation. We also unveil an endogenous activation and self-patterning of BMP-SMAD signaling during the ASE development in vitro. Together, Our findings reveal an unexplored developmental potential of hPSCs and highlight the self-organizing nature of post-implantation human embryogenesis. This study provides a novel and promising hPSC-based in vitro platform for advancing our fundamental understanding of early human development.
Figure 1. (a) Confocal micrographs showing differential hPSC morphogenesis and development in different niches. (b) Fold changes of amniotic markers in squamous cysts formed in Gel-3D. (c) Western blot showing endogenous BMP4 production in hPSC-amnion in Gel-3D. (d) Confocal micrograph showing nuclear staining of pSMAD1/5 in hPSC-amnion in Gel-3D. (e) Confocal micrographs showing inhibition of hPSC-amnion development by BMP inhibitor LDN193189. Scale bars, 50 µm.
31 2018 International Conference of Biomedical Information Perception & Microsystem A.05 Rong Fan Associate Professor Department of Biomedical Engineering Yale University New Haven, USA
Biography
Dr. Rong Fan is Associate Professor (tenured) of Biomedical Engineering at Yale University. He received a B.S. in Applied Chemistry from University of Science and Technology in China, a Ph.D. in Chemistry from the University of California at Berkeley, and then completed his postdoctoral training at California Institute of Technology, prior to launching his own research laboratory at Yale University in 2010. His current research interest is focused on developing single-cell micro/nano-technologies to interrogate functional cellular heterogeneity and inter-cellular signaling network in human health and disease (e.g., cancer and autoimmunity). He co-founded IsoPlexis, a life science tool company that aims to develop single-cell proteomics microchips for cancer immunotherapy monitoring and companion diagnostics. He is a member of Scientific Advisory Board (SAB) of Bio-Techne, a leading life science tools&reagents company. He is the recipient of numerous awards including the National Cancer Institute’s Howard Temin Career Transition Award, the NSF CAREER Award, and the Packard Fellowship for Science and Engineering.
32 2018 International Conference of Biomedical Information Perception & Microsystem BioMEMS tools for single-cell functional omics and the monitoring of cancer immunotherapy Rong Fan1, 2, 3* 1Department of Biomedical Engineering, Yale University, New Haven, CT 06520 2 Yale Stem Cell Center, Yale School of Medicine, New Haven, CT 06520 3 Yale Cancer Center, Yale School of Medicine, New Haven, CT 06520
*Email: [email protected]
Despite recent advances in single-cell genomic, transcriptional and mass cytometric profiling, it remains a challenge to collect highly multiplexed measurements of proteins produced from single cells for comprehensive analysis of immune functional state. My talk will be discussing a novel bioMEMS device technology for single-cell proteomic profiling, in particular, the co-detection of 40+ immune effector proteins such as cytokines/chemokines at the level of single cells, representing the highest multiplexing recorded to date for a single-cell protein secretion assay. I will describe how this microchip technology called IsoCode was conceived at the beginning, evolved over generations, further integrated with a fully automated single-cell processing platform called IsoLight comprising high-resolution optics, precision fluid handling and live cell incubation in the same system to truly enable robust and reproducible functional proteomics data at the single-cell level. It has been in the pipeline of commercialization at IsoPlexis and adopted by pharmaceutical companies like Novartis, Kite Pharma (a Gilead Company), Bellicum, and many others to evaluate their immunotherapy products. This microchip technology allowed for the full-spectrum dissection of T cell functions including genetically engineered chimeric antigen receptor T cells (CAR-T) in the treatment of patients with acute lymphoblastic leukemia or non-Hodgkin’s lymphoma. Our data obtained from a medium-scale clinical trial with CD19 CAR-T cells demonstrated strong association between CAR-T cells’ polyfunctionality (the ability for a single T cell to co-produce multiple immune effector proteins) and patient response, which opens up new opportunities for predicting not only therapeutic efficacy but also potentially life-threatening immunotoxicity. All these underscore the importance to measure functional proteomic heterogeneity even in phenotypically identical cell populations in order to evaluate the quality of cell-based therapeutics or to monitor patient responses for precision medicine. The small devices described above really enabled the wide-spread application in clinical monitoring of cancer immunotherapies.
33 2018 International Conference of Biomedical Information Perception & Microsystem A.06
Zuankai Wang Associate Professor Department of Mechanical and Biomedical Engineering City University of Hong Kong Hong Kong, China
Biography
Dr. Zuankai Wang is currently an associate professor in the Department of Mechanical and Biomedical Engineering at the City University of Hong Kong. He was awarded the Changjiang Chair Professor by Ministry of Education of China in 2016. He earned his Ph. D. degree in the Department of Mechanical, Aerospace and Nuclear Engineering at Rensselaer Polytechnic Institute in 2008. Dr. Wang has received many awards including the 2017 Outstanding Research Award and President’s Award at the City University of Hong Kong (2017, 2016), Outstanding Youth Award conferred by the International Society of Bionic Engineering (2016), OSA Young Scientist Award (2016), Chinese Government Outstanding Self-Financed Students Abroad Award (2007), and Materials Research Society (MRS) Graduate Student Silver Award (2007 Fall Meeting, Boston). His work on the invention of the most water repellent surface has been included in the Guiness Book of World Records. The Ph.D. students he supervised have won a number of prestigious awards including Young 1000 Talent Plan (2017, two PHD graduates), MRS Graduate Student Gold Award (2016 Fall Meeting, only two winners from China), Hiwin Doctoral Dissertation Award (2016), Hong Kong Young Scientist Award (2015), and MRS Graduate Student Silver Award (2015 Spring Meeting, only two winners from China).
34 2018 International Conference of Biomedical Information Perception & Microsystem Self-propelled droplets for biomedical applications Jiaqian Li1, Wanghuai Xu1, Xiaofeng Zhou2, Zuankai Wang1* 1Affiliation for First Author (Times New Roman,center, 10 lbs, single spacing) 2 Affiliation for First Author(Times New Roman,center, 10 lbs, single spacing) *Email: Corresponding author
1Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong 999077, China 2Science and Technology on Microsystem Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
*Email: [email protected] Abstract The ability to harvest various forms of ambient energy from ambient environments is of fundamental interest and technical importance. Over the past decade, various approaches, including piezoelectric, thermoelectric, electromagnetic and triboelectric, have been extensively exploited. And many studies have demonstrated that the liquid flowing on a solid surface produces an induced voltage and find a wide range of potential applications in energy harvesting and sensing. In this work, I will first discuss several strategies to generate self-propelled transport of droplets without the need of any external energy input (1). Then I will discuss the generation of electrical energy from a spontaneously self-propelled water droplet without the need of any external power supply (2). The spontaneous transport of a deposited droplet on a micro patterned surface with wettability gradient yields a voltage up to 64.7 mV, which can be further improved by increasing the droplet volume and stripe-wettability gradient. And quantitative calculation reveals that the droplet spontaneous motion is derived from the droplet interfacial energy gradient. This work will provide new ideas for optimizing the energy harvesting devices based on liquid-solid interface.
Figure 1: Schematic of the water Figure 2: Output voltage induced droplet self-motion on the by the self-propelled motion of 25 3-stripe patterned wettability uL droplet. gradient electrodes and wetting characterization. References
1. Jiaqian Li, Xiaofeng Zhou, Jing Li, Lufeng Che, Jun Yao, Glen McHale, Manoj Chaudhury, Zuankai Wang. Topological liquid diode. Science Advances, 3, eaao3530 (2017). 2. Chaoran Liu, Jing Sun, Xiaofeng Zhou, Zuankai Wang. Energy generation from a self-propelled droplet, Under review.
35 2018 International Conference of Biomedical Information Perception & Microsystem A.07
Yonggang Wen Associate Professor School of Computer Science and Engineering (SCSE) Nanyang Technological University (NTU), Singapore.
Biography
Dr. Yonggang Wen is an associate professor with School of Computer Science and Engineering (SCSE) at Nanyang Technological University (NTU), Singapore. He is the Associate Dean (Research) at College of Engineering (CoE) and the Acting Director of Nanyang Technopreneurship Centre (NTC) at NTU. He received his PhD degree in Electrical Engineering and Computer Science (minor in Western Literature) from Massachusetts Institute of Technology (MIT), Cambridge, USA, in 2008. Previously he has worked in Cisco to lead product development in content delivery network, which had a revenue impact of 3 Billion US dollars globally. Dr. Wen has published over 170 papers in top journals and prestigious conferences. His systems research has gained global recognitions. His work in Multi-Screen Cloud Social TV has been featured by global media (more than 1600 news articles from over 29 countries) and received ASEAN ICT Award 2013 (Gold Medal). His work on Cloud3DView for Data Centre Life-Cycle Management, as the only academia entry, has won the 2015 Data Centre Dynamics Awards – APAC (the ‘Oscar’ award of data centre industry) and 2016 ASEAN ICT Awards (Gold Medal). He is the winner of 2017 Nanyang Award for Innovation and Entrepreneurship, the highest recognition at NTU. He is a co-recipient of Best Paper Awards at 2016 IEEE Globecom, 2016 IEEE Infocom MuSIC Workshop, 2015 EAI Chinacom, 2014 IEEE WCSP, 2013 IEEE Globecom and 2012 IEEE EUC, and a co-recipient of 2015 IEEE Multimedia Best Paper Award. He serves on editorial boards for IEEE Communications Survey & Tutorials, IEEE Transactions on Multimedia, IEEE Transactions on Circuits and Systems for Video Technology, IEEE Wireless Communication, IEEE Transactions on Signal and Information Processing over Networks, IEEE Access Journal and Elsevier Ad Hoc Networks, and was elected as the Chair for IEEE ComSoc Multimedia Communication Technical Committee (2014-2016). His research interests include cloud computing, green data center, big data analytics, multimedia network and mobile computing.
36 2018 International Conference of Biomedical Information Perception & Microsystem Performance Optimization for Distributed Machine-Learning Applications at Scale: A Swiss-Army-Knife Approach Abstract Distributed machine-learning (ML) applications play an important role in fueling the emerging artificial intelligence revolution. In this context, the parameter server (PS) framework is widely used to train models at scale in modern ML systems, such as Petuum, MxNet, TensorFlow and Factorbird. It tackles the big-data problem by having worker nodes perform data-parallel computation, and having server nodes maintain globally shared parameters. However, when training models of large size, worker nodes frequently pull parameters from server nodes and push updates to server nodes, often resulting in high communication overhead. Our investigations show that modern distributed ML applications could spend up to 5 times more time on communication than computation. To address this problem, we propose an optimized communication layer for the PS framework, called as Parameter Flow (PF). The PS employs a Swiss-army-knife approach by staking three complementary techniques. First, we introduce an update-centric communication (UCC) model to exchange data between worker/server nodes via two operations: broadcast and push. Second, we develop a dynamic value-bounded filter (DVF) to reduce network traffic by selectively dropping updates before transmission. Third, we design a tree-based streaming broadcasting (TSB) system to efficiently broadcast aggregated updates among worker nodes. Our proposed PF can significantly reduce network traffic and communication time. Extensive performance evaluations have showed that PF can speed up popular distributed ML applications by a factor of up to 4.3 in a dedicated cluster, and up to 8.2 in a shared cluster, compared to a generic PS system without PF. The PF framework has been used by a few industry partners.
37 2018 International Conference of Biomedical Information Perception & Microsystem A.08
Yuan-Ting Zhang Chair Professor Biomedical Engineering at City University of Hong Kong, Hong Kong, China
Biography
Dr. Yuan-Ting Zhang is currently the Chair Professor of Biomedical Engineering at City University of Hong Kong. He was the Sensing System Architect in Health Technology at Apple Inc., California, USA in 2015, and the founding Director of the Key Lab for Health Informatics of Chinese Academy of Sciences till 2018. Professor Zhang dedicated his service to the Chinese University of Hong Kong from 1994 to 2015 in the Department of Electronic Engineering, where he served as the first Head of the Division of Biomedical Engineering and the founding Director of the Joint Research Center for Biomedical Engineering. Prof. Zhang was the Editor-in-Chief for IEEE Transactions on Information Technology in Biomedicine and the founding Editor-in-Chief of IEEE Journal of Biomedical and Health Informatics. He served as Vice Preside of IEEE EMBS, Technical Program Chair of EMBC’98 in Hong Kong, Conference Chair of EMBC’05 in Shanghai, International Committee Co-Chair of EMBC’07 in Lyon, Internationale Committee Chair of EMBC’ 11 in Boston, Internationale Committee Chair of EMBC’13 in Osaka, and Technical Program Co-Chair of EMBC’17 in Jeju Island. Prof. Zhang is currently the Editor-in-Chief for IEEE Reviews in Biomedical Engineering, Chair of 2018 Gordon Research Conference on Advanced Health Informatics, Chair of the Working Group for the development of IEEE 1708 Standard on Wearable Cuffless Blood Pressure Measuring Devices, and Chair of 2016-2018 IEEE Award Committee in Biomedical Engineering. Prof. Zhang's research interests include cardiovascular health informatics, unobtrusive sensing and wearable devices, neural muscular modeling and pHealth technologies. He was selected on the 2014, 2015, 2016 and 2017 lists of China’s Most Cited Researchers by Elsevier. He won a number of international awards including IEEE-EMBS best journal paper awards, IEEE-EMBS Outstanding Service Award, IEEE-SA 2014 Emerging Technology Award. Prof. Zhang is elected to be IAMBE Fellow, IEEE Fellow and AIMBE Fellow for his contributions to the development of wearable and m-Health technologies.
38 2018 International Conference of Biomedical Information Perception & Microsystem Health Engineering: Wearable “MINDS” for the Early Prediction of Cardiovascular Diseases
Yuan-Ting Zhang Department of Biomedical Engineering at City University of Hong Kong
ABSTRACT:
This talk will outline some of our research work in health engineering which attempts a convergence approach to integrate technologies across multiple scales in the biological hierarchy from molecular, cell, organ to system for the prediction of cardiovascular diseases (CVDs). The p r e s e n t a t i o n w i l l f o c u s o n t h e d e v e l o p m e n t o f
u 39 n o b t r 2018 International Conference of Biomedical Information Perception & Microsystem A.09
Leslie Yeo Professor & Australian Research Council Future Fellow School of Engineering RMIT University, Melbourne, Australia
Biography
Leslie Yeo is currently an Australian Research Council Future Fellow, Professor of Chemical Engineering and Director of the MicroNanomedical Research Centre at RMIT University, Australia. He received his PhD from Imperial College London in 2002, for which he was awarded the Dudley Newitt prize for a computational/theoretical thesis of outstanding merit. Prior to joining RMIT University, he was a Mathematical Modeller at Det Norske Veritas UK and a postdoctoral research associate in the Department of Chemical & Biomolecular Engineering at the University of Notre Dame, USA, after which he held a faculty position at Monash University. Dr Yeo was the recipient of the 2007 Young Tall Poppy Science Award from the Australian Institute for Policy & Science ‘in recognition of the achievements of outstanding young researchers in the sciences including physical, biomedical, applied sciences, engineering and technology’, and both the Dean’s and Vice-Chancellor’s awards for excellence in early career research at Monash University. His work on microfluidics has been featured widely in the media, for example, on the Australian Broadcasting Corporation’s science television program Catalyst, 3RRR and SBS radio broadcasts, and in various articles in The Economist, New Scientist and The Washington Times, in addition to being highlighted in Nature and Science. Dr Yeo is co-author of the book Electrokinetically Driven Microfluidics & Nanofluidics (Cambridge University Press), and the author of over 190 research publications and 20 patent applications. He is also the Editor of the American Institute of Physics journal Biomicrofluidics and an editorial board member of Interfacial Phenomena & Heat Transfer and Scientific Reports.
40 2018 International Conference of Biomedical Information Perception & Microsystem Plug-and-Actuate On Demand: Multimodal Modular Individual Addressability of Microarray Plates Amgad R. Rezk,1 Leslie Y. Yeo1 1 Micro/Nanophysics Research Laboratory, School of Engineering, RMIT University Melbourne, VIC 3001, Australia *Email: [email protected]
Despite considerable advances in microfluidic platforms over the past two decades, the ubiquitous microarray titre plate remains a stalwart for compound screening and biochemical analysis. This can partly be due to the aversion of laboratory practitioners to new technology or protocols, which can often be perceived as unnecessarily complex, even if they are more efficient or cost effective. Alternatively, this may simply be due to the compatibility of existing equipment and methods with the array of ancillary technology such as microplate readers and microscopes that are already available in the laboratory, so as to avoid the need to invest in the infrastructure costs and training resources associated with the procurement of new equipment to accommodate new formats and protocols. To overcome such inertia in the uptake of new technology, we have developed a reconfigurable modular acoustofluidic platform that seamlessly interfaces with, and allows individual addressability of the ubiquitous microarray well plate that is a mainstay of drug development workflows. It is our belief that laboratory practitioners outside the microfluidics community are more open to adopting new technology if these are integrated into existing laboratory formats equipment they are familiar with and have invested heavily in. While addressability of individual wells in a microarray plate using acoustic waves was claimed in two recent works (Alhasan et al., 2016; Kurashina et al., 2017), only conceptual proof was provided in both of these papers in which only a single device was interfaced with one well. That the ability to individually address all of the wells on the plate was not shown, let alone on the industry standard 96-well plate format, was clearly due to fundamental limitations in these technologies. We discuss these limitations, and show how they can be circumvented with the proposed platform such that true individual addressability of single wells, or even simultaneous/serial addressability of multiple wells for driving a range of microfluidic operations in the well(s) on demand can be achieved using a novel class of hybrid surface and acoustic waves that have only just been discovered (Rezk et al., 2016).
Alhasan L, et al. Rapid Enhancement of Cellular Spheroid Assembly by Acoustically Driven Microcentrifugation. ACS Biomat Sci Eng 2016, 2:1013–1022. Kurashina Y, et al. Cell Agglomeration in the Wells of a 24-Well Plate Using Acoustic Streaming. Lab Chip 2017, 17:876–886. Rezk AR, et al. HYbriD Resonant Acoustics (HYDRA). Adv Mater 2016, 28: 1970–1975.
41 2018 International Conference of Biomedical Information Perception & Microsystem A.10
Nam-Trung Nguyen Associate Professor Griffith University, Brisbane, Australia
Biography
Nam-Trung Nguyen received his Dip-Ing, Dr Ing and Dr Ing Habil degrees from Chemnitz University of Technology, Germany, in 1993, 1997 and 2004, respectively. The habilitation degree (Dr Ing Habil ) is the qualification for a full professorship in Germany. In 1998, he was a postdoctoral research engineer in the Berkeley Sensor and Actuator Center (University of California at Berkeley, USA). Prof Nguyen is the First Runner Up of Inaugural ProSPER.Net-Scopus Young Scientist Awards in Sustainable Development in 2009 and the Runner Up of ASAIHL-Scopus Young Scientist Awards in 2008. He is a Fellow of ASME and a Member of IEEE. Nguyen’s research is focused on microfluidics, nanofluidics, micro/nanomachining technologies, micro/nanoscale science, and instrumentation for biomedical applications. He published over 340 journal papers with over 13,000 citations (Google Scholar) and field 8 patents, of which 3 were granted. Among the books he has written, the first and second editions of the bestseller “Fundamentals and Applications of Microfluidics” were published in 2002 and 2006, respectively. His latest book “Nanofluidics” was published in 2009. The second edition of the bestselling book “Micromixer” was acquired and published by Elsevier in 2011.
42 2018 International Conference of Biomedical Information Perception & Microsystem Liquid marble based digital microfluidics: fundamental physics and applications
Nam-Trung Nguyen1 1Queensland Micro and Nanotechnology Centre (QMNC), Griffith University, 170 Kessels Road, Nathan, QLD 4011, Australia
Abstract
Liquid marbles (LMs) are droplets with volume of typically microliters coated with hydrophobic powder. The versatility, ease of use and low cost make LMs an attractive platform for digital microfluidics.1 The talk will report our recent discoveries in the physics of liquid marbles (LMs) that allow them to serve as miniature labs. First, a novel LM manipulation technique using dielectrophoresis (DEP) will be reported. The manipulation technique based on DEP suits any material as it only requires a permittivity mismatch between the LM and its surrounding, which exists in almost all practical cases. The DEP setup consists of a small electrode connected to a high direct-current voltage, positioned above the LM to be manipulated. Energising the electrode generates a strong and non-uniform electric field which results in DEP of the LM. LMs with volume up to 25 μL can be lifted, transported and released with this technique. Coalescence is achieved by dropping a lifted LM on another sessile one. Using this manipulation technique, the coalescence of two LMs was demonstrated via vertical collision. With the DEP technique, the distance between LMs and the horizontal offset can be precisely controlled as the optimisation parameters of the coalescence process. A scaling law and an operation map to predict the success rate of coalescence, which allows LMs to serve as practical micro bioreactors or micromixers. Next, the robustness of LMs was investigated in terms of evaporation and structural deformation. We demonstrated that a LM cluster can survive thermal cycling conditions up to 95°C in a humidity controlled environment. Survival at elevated temperature makes a LM feasible for its use in polymerase chain reaction (PCR). The robustness of a LM in terms of elasticity was also investigated. The deformation of a LM was quantitatively described resorting to geometrical approximations. The elasticity of a LM was determined using the parallel plate compression test with a large strain (ε≈0.7). The liquid marble is placed between a precisely z-axis controlled plate and a precision scale. The compression force measured with the precision scale was use to evaluate the compressive stress. Images of the deformed liquid marble are recorded and processed to determine the vertical strain. Results from the compression experiments were compared with simulation based on coarse grained molecular dynamics. The above proof of concept and findings lay the foundation for the use of liquid marbles as a robust digital microfluidic platform with applications such as large-scale three-dimensional cell culture and high-throughput digital PC
43 2018 International Conference of Biomedical Information Perception & Microsystem
B. Sub-Forum 1: "Electronic Information &Biomedicine" Outstanding Talent Forum
44 2018 International Conference of Biomedical Information Perception & Microsystem B.01
Dezhong Yao Professor Center for Information in Medicine University of Electronic Science and Technology of China
Biography
AIMBE Fellow (2017), Vice-President of the Chinese Society of Biomedical Engineering, Outstanding Youth Reward of National Science Foundation of China (2005), and Changjiang Scholar Professor of the Ministry of Education of China (2006); He got Bachelor Degree(1985) in Physics from Southwest University in Chongqing, Master Degree(1988 ) in Agriculture from Zhejiang University in Hangzhou, and PhD Degrees(1991, 2005) in Geophysics and Biomedical Science from Chengdu University of Technology and Aalborg University, respectively. He has been a Faculty Member with UESTC since 1993; a full Professor since 1995; the Dean of the School of Life Science and Technology（2001-2017）. He was a Visiting Scholar at University of Illinois at Chicago, IL, from September 1997 to August 1998, and a Visiting Professor at McMaster University, Hamilton, ON, Canada, from November 2000 to May 2001. He is the author or coauthor of about 200 papers with Google Scholar >6000 citations and h=41. Main contributions: EEG zero reference (REST) which may be used to standardize EEG reference to a neutral point at Infinity, recommanded by IFCN for EEG analysis(www.neuro.uestc.edu.cn/rest); scale-free music of the brain which may translate brainwave into music according to the scale-free property followed by both EEG and music(http://www.neuro.uestc.edu.cn/brainmusic.html), and EEG-fMRI information fusion with application in Epilepsy. His current interest is multi-modal imaging Methods and various applications.
45 2018 International Conference of Biomedical Information Perception & Microsystem Brain Science and Cloud platform for Brainformatics Dezhong Yao, Li Dong, Jianfu Li
The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, China
Corresponding author: Dezhong Yao e-mail: [email protected] Tel: 86-28-61830654
Abstract Brain science is undergoing a protocol revolution from the traditional principal investigator (PI) based experiment centralized study to a new era with big data analysis and experiment together, and new platform for the new age is necessary. Here a cloud platform for brainformatics[1] is introduced, it is WeBrain (webrain.uestc.edu.cn) (Figure 1) designed to integrate big data, tools for big data analysis and computation resources, it is one of the main content of the CCC-axis initiated in 2018 by NSFC(China)-FRQ(Canada)- CITMA(Cuba). Special tools for zero EEG reference (REST：www.neuro.uestc.edu.cn/rest) and simultaneous EEG-fMRI fusion（NIT: http://www.neuro.uestc.edu.cn/NIT.html）are introduced with examples.
Figure 1. China-Cuba-Canada tri-nations cooperation and its connections to the world.
1.Yao D. Mesoscopic Brainformatics. Y Zeng et al. (Eds.), BI 2017, LNAI 10654,pp.315-324, 2017. 2.Dong L, Li F, Liu Q, Wen X, Lai Y, Xu P and Yao D (2017) MATLAB Toolboxes for Reference Electrode Standardization Technique (REST) of Scalp EEG. Front. Neurosci. 11:601. doi: 10.3389/fnins.2017.00601
46 2018 International Conference of Biomedical Information Perception & Microsystem B.02
Xi Xie Professor School of Electronic and Information Technology Sun Yat-Sen University
Biography:
Dr. Xi Xie got his Ph.D degree in 2014 at Stanford University in Prof. Nick Melosh’s lab, and got postdoctoral training at Massachusetts Institute of Technology in Prof. Robert Langer and Prof. Dan Anderson’s lab from 2014-2016. In 2016, he was awarded with National Thousand Youth Talents Plan (China), and has been working as Professor in School of Electronic and Information Technology at Sun Yat-Sen University, and as adjunct professor in The First Affiliated Hospital of Sun Yat-Sen University. Dr. Xie’s lab has been working on nanodevices and nanomaterials for biomedical application, flexible electronics and bioelectronics, wearable electronics, and biomedical engineering. He has published many paper on high impact journals such as ACS Nano and Nano Letters as first author or corresponding author.
47 2018 International Conference of Biomedical Information Perception & Microsystem
Nanostraw-device for highly efficient gene transfection
Xi Xie1*, Hui-Jiuan Chen, Tian Hang, Jiangming Wu, Chengduan Yang School of Electronic and Information Technology, Sun Yat-Sen University; The first Affiliated Hospital of Sun Yat-Sen University *[email protected]
Abstract Nanowires have a wide variety of biomedical applications including drug delivery, cellular manipulation, cellular interfacing, and bio-sensing. Specifically, hollow nanowire arrays, or "nanostraws", are recently developed to penetrate cell membranes and to provide temporal and dose controlled delivery of biomolecules into the cells. The fabrication of nanostraw arrays use nanoporous membranes as starting templates. However, nanoporous membranes with custom-designed diameters and densities are often expensive or commercially unavailable. As a result, nanostraws reported in literature consist of a limited number of structures with restricted applications. In this work, a simple approach of preparing nanostraws with differing structures, including various diameters, lengths, and densities, was developed. Furthermore, the application of nanostraws in gene transfection was assessed. To fabricate the nanostraw arrays, O2 plasma etching was applied to the template membrane with a desirable pore density to produce nanopores with matching sizes. Then, a series of nanostraws with controllable diameters, densities, and lengths was consistently fabricated. Finally, the nanostraws were integrated with a microfluidic device to penetrate the cell membrane or coupled with external techniques to achieve DNA transfection. This work demonstrated a simple yet versatile approach to fabricate a variety of hollow nanowires for broad biomedical device applications.
48 2018 International Conference of Biomedical Information Perception & Microsystem B.03
Weiwei Zhao Professor School of Material Science and Engineering Harbin Institute of Technology, Shenzhen
Biography
Weiwei Zhao received BE degree in Materials Engineering from Harbin Institute of Technology in 2002, and Ph.D. degree also in Materials Engineering from Harbin Institute of Technology in 2008. He is currently a professor with School of Material Science and Engineering in Harbin Institute of Technology, Shenzhen. He has published more than 20 journal papers, including Nature Materials, Nature Physcis, Science Advances, Nano Letters, PRL etc. His research interests cover mainly quantum materials and devices, printed electronics.
49 2018 International Conference of Biomedical Information Perception & Microsystem A general approach for flexible and foldable low-resistivity paper/fabric-based electronics
Weiwei Zhao1,2* 1Research Center of Flexible Printed Electronic Technology, Harbin Institute of Technology, Shenzhen, 518000, P. R. China 2State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology, Harbin, 150000, P. R. China *Email: [email protected]
Abstract Paper and fabric substrates were modified by various paste, and then many types of metal nano or micro particles are able to be printed on these modified flexible substrates to fabricate electronic patterns by sintered at temperatures even lower than 100℃. The paper/fabric-based flexible electronics possess brilliant conductivity, flexibility and foldability, and they are light (paper is about 13% of the whole mass of paper flexible electronics), which can be convenient, portable and save space1. All these merits can promote the development and application of flexible and foldable electronics, such as paper/fabric-based flexible sensing electronics2. We also found the difference of work function between the metal particles and the modified substrate can help reducing the sintering temperature, this also help to enhance the flexibility and foldability of the electronics on paper or fabric substrates.
[1] Yun J, Lim Y, Lee H, et al. A Patterned Graphene/ZnO UV Sensor Driven by Integrated Asymmetric Micro‐Supercapacitors on a Liquid Metal Patterned Foldable Paper[J]. Advanced Functional Materials, 2017, 27(30): 1700135. [2] Khan Y, Ostfeld A E, Lochner C M, et al. Monitoring of Vital Signs with Flexible and Wearable Medical Devices[J]. Advanced Materials, 2016, 28(22):4373.
50 2018 International Conference of Biomedical Information Perception & Microsystem B.04
Yufei Liu Professor College of Optoelectronic Engineering in Chongqing University
Biography
Dr. Yufei Liu is a Professor of College of Optoelectronic Engineering in Chongqing University, Director of the Centre for Intelligent Sensing Technology, PI for National Key Research and Development Program of China, and the member of national thin-layer graphite material standardization technical committee. Dr. Yufei Liu was awarded bachelor's degrees in both physics and economics from Peking University in 2003, and Ph.D. in Electronic Engineering from Heriot-Watt University in Scotland in 2011, then worked at Swansea University and Imperial College and published more than 30 academic papers and applied for more than 10 domestic and foreign invention patents. Dr. Liu Yufei’s knowledge transfer project has successfully achieved sales of more than US $8 million, and was nominated by the British Technology Strategy Board (TSB) for the Bussiness Leader of Tomorrow Award.
51 2018 International Conference of Biomedical Information Perception & Microsystem Integrated Microsystems for Early Diagnosis and Precision Bio-Medical Application Yufei Liu1,* 1 Centre for Intelligent Sensing Technology, College of Optoelectronic Engineering, Chongqing University, Chongqin, 400044, China. *Email: [email protected];
Based on the explosive demands of the coming ageing society, early stage diagnostic and precise bio-medical treatment has been listed as one of ten industries, priority promoted in the Chinese "Thirteenth Five-Year Plan". With the gradual exploration of the human body, the study for intricate structure, characteristics, functions and clinical of the human heart, blood vessels, brain and nerves has been carried out. As innovative drug development, early diagnosis, pathological screening platforms, and minimally invasive precision treatment device platforms have become an urgent need for medical workers and patients. Based on advanced micro-nano fabrication technologies, micro-electromechanical system technology is expanding from the traditional micro-mechanical and microelectronics fields to the biomedical field, such as advanced diagnostic kit platforms, cell screening platforms, and precision surgical instruments. This report mainly takes the neural microelectrode arrays and microfluidic based detection kits as examples and briefly describes and discusses integrated microsystem technologies for early diagnosis and precise treatment, then summarizes and explores innovative applications in areas such as minimally invasive neurosurgery surgical instruments, skin repair, and portable hepatitis testing devices.
52 2018 International Conference of Biomedical Information Perception & Microsystem B.05
Xiaochen Dong Professor Nanjing Tech University Winners of national outstanding youth science fund
Biography
Xiaochen Dong received Ph.D. degree from Zhejiang University in 2007. He is currently a professor with Department of Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University. He has published more than 180 journal papers. His research interests cover mainly biophotonics, carbon-based nanomaterials, semiconductor electronic devices, new energy materials, electrochemical biosensors, etc.
53 2018 International Conference of Biomedical Information Perception & Microsystem NIR Photosensitizers for Targeted Cancer Phototherapy Xiaochen Dong Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM) Nanjing Tech University, 30 South Puzhu Road, Nanjing 211800, China. *Email: [email protected]
Abstract text The development of effective cancer treatment methods has become extremely urgent due to the rapid growth of cancer patients. Comparing to conventional therapeutic methods for cancer, photothermal therapy (PTT) and photodynamic therapy (PDT) are two main noninvasive phototherapies due to the low side-effects, high selectivity and efficiency. PTT and PDT are usually induced by near-infrared (NIR) light then the excited agents inside tumor can generate heat or reactive oxygen species (ROS) to kill tumor cells. However, most of inorganic agents face big challenges of the potential far-reaching toxicity and bio-refractory in clinic. Differently, small molecular organic dyes as photothermal agents usually presents low-toxicity, good biodegradability and fluorescence in biological tissue. Herein, we have designed and synthesized a series of NIR-absorbing photosensitizers (PSs), based on DPP and Bodipy derivatives with excellent tumor-targeting performance and high ROS generation ability or photothermal conversion. Moreover, the NPs can be passive targeted to tumor sites by the enhanced permeability and retention (EPR) effect and photoacoustic imaging can visualize the tumor site for real-time monitoring during the therapeutic process. What’s more, by the assistance of pharmacokinetics, the properties and mechanisms of NIR-absorbing Nano-PSs were studied, which provided a more effective and smart method for accurate imaging of cancer tumor and phototherapy.
54 2018 International Conference of Biomedical Information Perception & Microsystem B.06
Ma Xing Professor or School of Materials Science and Technology Photo Harbin Institute of Technology, Shenzhen
Biography
Dr. Xing Ma obtained his Ph.D. degree in Materials Science and Engineering from Nanyang Technological University in 2013. He used to work as Alexander von Humboldt research fellow at Max-Planck Institute for Intelligent Systems (MPI-IS) at Stuttgart, Germany, from 2014 to 2016. His research interest focuses on enzyme powered mesoporous silica micro/nanomotors for active drug delivery, and nano-devices for bio-sensing. He has been awarded the China Thousand Talents Plan for Young Scholars Award (2016) and Shenzhen Peacock Talent Program of Category B (2017). In 2016, Dr. Xing Ma was awarded the Günter Petzow Prize from MPI-IS for his achievement in enzyme powered micro/nano-motors. Up to now, he has published more than 50 papers, with citations more than 2500 and H-index 29.
55 2018 International Conference of Biomedical Information Perception & Microsystem Micro/Nano-Motors for Biomedical Applications Xing Ma1,* 1 Materials Science and Engineering School, Harbin Institute of Technology (Shenzhen), 518055, China *Email: [email protected]
Self-propelled micro/nano-motors (MNM) with cargo loading capability can lead to a new solution for active target drug delivery.[1] However, such active drug delivery system should be biocompatible in terms of fabrication materials, as well as fuel providing the energy to power their self-propulsion. Mesoporous silica has been proved to be a useful material for drug/gene delivery for cancer therapy both in vitro and in vivo, in virtue of unique mesoporous structure, tunable nano-size, and biocompatibility. Enzymes triggered bio-catalytic reactions have been regarded as most promising alternative to replace traditional propulsion strategy based on Pt/H2O2,[2] because of versatile enzyme/fuel combinations, e.g. catalase/H2O2, non-toxic glucose oxidase (GOx)/glucose and urease/urea.[3] Hereby, by integration between mesoporous silica and enzyme triggered bio-catalytic reactions, we successfully fabricated biocompatible MNM capable of self-propulsion by consuming non-toxic fuel.[3,4] We also achieved reversible velocity control on the motors by manipulating the enzymatic activity with inhibitors/re-activation molecules.[4] Magnetic guidance was utilized to control the motors’ movement direction, towards target locations. The nanopores of the mesoporous silica with internal pore diameter about 2-3 nm can be utilized for drug loading and delivery in large quantity. Sustained release of drug molecules from these mesoporous MNM indicates great potential of using them for active target drug delivery in future biomedical applications.
Figure 1. Schematic illustration of motion control of urea powered biocompatible mesoporous silica hollow microcapsules.[4]
References. [1] Xing Ma, Kersten Hahn, Samuel Sanchez. J. Am. Chem. Soc. 2015, 137, 4976–4979. [2] Xing Ma and Samuel Sanchez, Chem. Commun. 2015, 51, 5467-5470. [3] Xing Ma, Anita Jannasch, Urban-Raphael Albrecht, Kersten Hahn, Albert Miguel López, Erik Schäffer and Samuel Sanchez. Nano Lett. 2015, 15, 7043–7050. [4] Xing Ma, Xu Wang, Kersten Hahn, Samuel Sanchez. ACS Nano. 2016, 10, 3597–3605.
56 2018 International Conference of Biomedical Information Perception & Microsystem B.07
Photo Zhizhao Che Professor State Key Laboratory of Engines Tianjin University
Biography
Zhizhao Che received his BEng and MEng degrees from Harbin Institute of Technology in 2005 and 2007, respectively, and obtained his PhD degree from Nanyang Technological University (Singapore) in 2012. He did postdotoral research at Nanyang Technological University and then at Imperial College London (UK) until January 2016. He is currently a professor in the State Key Laboratory of Engines, Tianjin University. His main research interest is flow dynamics and heat/mass transfer of droplets, and he has published more than 30 SCI-indexed journal papers in this area. He is also a recipient of the “National 1000-Talent Youth Program”.
57 2018 International Conference of Biomedical Information Perception & Microsystem Heat and mass transfer in simple and compound droplets moving in microchannels Zhizhao Che1*, Teck Neng Wong2 , Nam-Trung Nguyen3, Tianyou Wang1 1 State Key Laboratory of Engines, Tianjin University, Tianjin, 300072, China 2 School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore 3 Queensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Brisbane, QLD 4111, Australia *Email: [email protected]
Understanding the flow field, heat transfer, and mass transfer within droplets moving in microchannels is an important task for paving the way for related applications. Complex vortex structures are produced by the presence of the droplet interface and the confining effect of the microchannels, and these structures have a significant impact on the heat and mass transfer. Here we unveil the vortex structures in droplets moving in microchannels. The heat and mass transfer processes in droplets under different conditions are analyzed, and the effects of the vortex structures and the interface shapes are investigated, respectively. The liquid lubrication film between the droplet and the channel wall is found to significantly affect the heat and mass transfer. Particular attention is paid to the flow and mass transfer in compound droplets, which are useful in the encapsulation and compartmentalization of substances. The shell of compound droplets can serve as a protective layer of the inner phase by minimizing the mass transfer between the core and the outer phase. The leakage of the species from the core to the continuous phase via the shell of the compound droplet is also analyzed.
Figure 1 Flow field of simple and compound droplets.
References Che Z, Wong TN, Nguyen NT, Yang C (2015). Three dimensional features of convective heat transfer in droplet-based microchannel heat sinks. International Journal of Heat and Mass Transfer, 86, 455-464. Che Z, Yap YF, Wang T (2018). Flow structure of compound droplets moving in microchannels. Physics of Fluids, 30, 012114.
58 2018 International Conference of Biomedical Information Perception & Microsystem B.08
Huajin Tang Professor College of computer science Sichuan University
Biography
Huajin Tang received the B.Eng. degree from Zhejiang University, M.Eng. degree from Shanghai Jiao Tong University, and Ph.D. degree from the National University of Singapore in 1998, 2001, and 2005, respectively. He was an R&D Engineer with STMicroelectronics, Singapore from 2004 to 2006. From 2006, he was a Postdoctoral Fellow with Queensland Brain Institute, University of Queensland, Australia. Since 2008 he was the Lab Head of Robotic Cognition at the Institute for Infocomm Research, A*STAR, Singapore. Currently he is National Youth-1000 Talent Distinguished Professor and Director of the Neuromorphic Computing Research Center, Sichuan University, China. His research interests include neuromorphic computing and hardware, neuro-robotics, etc. He received IEEE Outstanding TNNLS Paper Award 2016. He has served as Associate Editor for IEEE Trans. On Neural Networks and Learning Systems, IEEE Trans. on Cognitive and Developmental Systems, and Frontiers in Neuromorphic Engineering, and Program Chair for CIS-RAM 2015, 2017, and ISNN 2019, etc.
59 2018 International Conference of Biomedical Information Perception & Microsystem Title of presentation (Times New Roman, Center, 14 lbs, bold, single spacing, paragraph spacing 0.5 lines before) First Author1 (presenting author underscored), Co-authors2* (Times New Roman ,center, 12 lbs, single spacing) 1Affiliation for First Author (Times New Roman,center, 10 lbs, single spacing) 2 Affiliation for First Author(Times New Roman,center, 10 lbs, single spacing) *Email: Corresponding author
Abstract text (Times New Roman 12 lbs, single spacing, justify). The maximum size for the abstract is one page including figures and references. Please start with saving this Word template on your computer and then use the saved file to prepare your abstract document. Name your abstract document "LastnameFirstname_ ICBIPM2018 " and upload it to the site [www.icbipm2018.medmeeting.org] in the upload section. We only accept abstracts in word format. Please pay attention to choose the appropriate topic in the process of uploading. One representative figure is preferred (figure resolution should be 300 dpi).
2-3 references are preferred. Wang LM, et al. Selective Targeting of Gold Nanorods at the Mitochondria of Cancer Cells: Implications for Cancer Therapy. Nano lett. 2011, 11:772-780. (Times New Roman 10 lbs, single spacing, justify) Please pay attention to the type setting format: top, lower, left and right margins 2.54 cm; header and footer 2 cm.
60 2018 International Conference of Biomedical Information Perception & Microsystem B.09
Tuan Guo Professor Institute of Photonics Technology Jinan University, China Email: [email protected]
Biography
Tuan Guo is a Professor in the Institute of Photonics Technology, Jinan University, Guangzhou, China. He received the Ph.D. in Optics from Nankai University in 2007. Thereafter he worked as a Postdoctoral Fellow with the Department of Electronics at Carleton University (Canada) and the Photonics Research Centre at The Hong Kong Polytechnic University. He joined the Jinan University as an Associate Professor in 2011 and promoted to a full Professor in 2014. He has authored and coauthored more than 150 papers in the peer-reviewed international journals (included 6 invited review papers) and presented over 30 invited talks at international and national conferences. He holds 15 patents and pending patents. His research activities include optical fiber sensors, fiber lasers, fiber gratings, plasmonics, biophotonics, optofluidics. He was a Co-Chair of the IEEE-IMS Technical Committee “Photonic Technology in Instrumentation and Measurement”, Associated Editor for Journal of Sensors, Guest Editor of MDPI Sensors and Guest Editor of IEEE/OSA Journal of Lightwave Technology. Dr. Guo is a Senior Member of IEEE and a Senior Member of OSA.
61 2018 International Conference of Biomedical Information Perception & Microsystem Plasmonic fiber-optic biochemical and electrochemical sensing Tuan Guo Institute of Photonics Technology, Jinan University, Guangzhou 510632, China *Email: [email protected]
Abstract: Surface Plasmon resonance (SPR) optical fiber sensors can be used as a cost-effective and relatively simple-to-implement alternative to well established bulky prism configurations for in-situ high sensitivity biochemical and electrochemical measurements. The miniaturized size and remote operation ability offer them a multitude of opportunities for single-point sensing in hard-to-reach spaces, even possibly in vivo. Grating-assisted and polarization control are two key properties of fiber-optic SPR sensors to achieve unprecedented sensitivities and limits of detection. The biosensor configuration presented here utilizes a nano-scale metal-coated tilted fiber Bragg grating (TFBG) imprinted in a commercial single mode fiber core with no structural modifications. Such sensor provides an additional resonant mechanism of high-density narrow cladding mode spectral combs that overlap with the broader absorption of the surface Plasmon for high accuracy interrogation. In this talk, we briefly review the principle, characterization and implementation of plasmonic TFBG sensors, followed by our recent developments of the “surface” and “localized” affinity studies of the biomolecules for real life problems, the electrochemical actives of energy storage devices for renewable energy storage, and ultra-highly sensitive gas detection.
References
[1] C. Caucheteur, T. Guo, and J. Albert, “Review of plasmonic fiber optic biochemical sensors: improving the limit of detection,” Analytical and Bioanalytical Chemistry, vol. 407, no. 14, pp. 3883-3897, May 2015. [2] Christophe Caucheteur*, Tuan Guo, Fu Liu, Bai-Ou Guan, Jacques Albert*, “Ultrasensitive plasmonic sensing in air using optical fibre spectral combs”, Nature Communications, 2016; 7: 13371. DOI: 10.1038/ncomms13371. [3] Jiajie Lao, Peng Sun, Fu Liua, Xuejun Zhang, Chuanxi Zhao, Wenjie Mai, Tuan Guo, Gaozhi Xiaod and Jacques Albert, “In Situ Plasmonic Optical Fiber Detection of the State of Charge of Supercapacitors for Renewable Energy Storage”, Light: Science & Applications, accepted.
62 2018 International Conference of Biomedical Information Perception & Microsystem B.10
Wei Liu Professor School of Physics and Technology Wuhan University
Biography
LIU Wei received Bachelor, Master and Ph.D. degree from School of Physics and Technology, Wuhan University. He got Ph.D. degree in 2008. He was a visited scholar in Hopkins University ,USA, in 2015. He is currently an associate professor with School of Physics and Technology, Wuhan University. He has published more than 50 journal papers. His research interests cover mainly integrated circuit, photoelectron concerned machine and microelectronics equipment.
Presentation Title：
Enhanced cancer immunotherapy by cancer cell membrane camouflaged nanoparticles
Although the anti-PD-1 immunotherapy has been widely used to treat melanoma, its efficacy still needs to be improved. We designed mesoporous silica nanoparticles (MSN) that encapsulate cancer cell membrane and glucose oxidase (GOx) to realize starvation therapy. By functionalizing the MSN’s biomimetic surface, we are able to synthesize nanoparticles that can escape host immune system and target homotypic antigens. These attributes indicates our nanoparticles have improved cancer targeting and enrichment in tumor issues. Our synthetic CMSN-GOx complex can ablate tumors and produce tumor-associated antigens from cancer cell membrane to stimulate anti-tumor immune response. We performed in vivo analysis of our nanoparticles and demonstrated that our combined therapy, CMSN-GOx plus PD-1, shows better anti-tumor therapeutic effect, compared with using CMSN-GOx or PD-1 alone. Additionally, this work introduces Positron Emission Tomography imaging to evaluate the cancer therapy in vivo by measuring level of glucose metabolism in tumor tissues.
63 2018 International Conference of Biomedical Information Perception & Microsystem Enhanced cancer immunotherapy by cancer cell membrane camouflaged nanoparticles Wei Xie1, Wei Liu 1* 1 Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, China *Email: [email protected] Abstract Although the anti-PD-1 immunotherapy has been widely used to treat melanoma, its efficacy still needs to be improved. We designed mesoporous silica nanoparticles (MSN) that encapsulate cancer cell membrane and glucose oxidase (GOx) to realize starvation therapy. By functionalizing the MSN’s biomimetic surface, we are able to synthesize nanoparticles that can escape host immune system and target homotypic antigens. These attributes indicates our nanoparticles have improved cancer targeting and enrichment in tumor issues. Our synthetic CMSN-GOx complex can ablate tumors and produce tumor-associated antigens from cancer cell membrane to stimulate anti-tumor immune response. We performed in vivo analysis of our nanoparticles and demonstrated that our combined therapy, CMSN-GOx plus PD-1, shows better anti-tumor therapeutic effect, compared with using CMSN-GOx or PD-1 alone. Additionally, this work introduces Positron Emission Tomography imaging to evaluate the cancer therapy in vivo by measuring level of glucose metabolism in tumor tissues.
Fig.1 Schematic illustration of anti-tumor immune response and enhanced anti-PD-1 immunotherapy induced by CMSN-GOx.. References 1. Rao, L.; Bu, L.-L.; Cai, B.; Xu, J.-H.; Li, A.; Zhang, W.-F.; Sun, Z.-J.; Guo, S.-S.; Liu, W.; Wang, T.-H.; Zhao, X.-Z., Cancer Cell Membrane-Coated Upconversion Nanoprobes for Highly Specific Tumor Imaging. Advanced Materials 2016, 28, 3460-3466. 2. Wang, C.; Xu, L.; Liang, C.; Xiang, J.; Peng, R.; Liu, Z., Immunological Responses Triggered by Photothermal Therapy with Carbon Nanotubes in Combination with Anti-CTLA-4 Therapy to Inhibit Cancer Metastasis. Advanced Materials 2014, 26, 8154-8162..
64 2018 International Conference of Biomedical Information Perception & Microsystem
C. Biomedical Materials and Micro-nano Devices Forum Meeting
65 2018 International Conference of Biomedical Information Perception & Microsystem C.01
Hejun Du Associate Professor School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore
Biography
DU obtained both BEng and MEng from Nanjing University of Aeronautics and Astronautics, China in 1983 and 1986, respectively. Subsequently, he lectured there until he left for Imperial College of Science, Technology and Medicine, UK for his PhD study in 1988. Du joined Nanyang Technological University, Singapore in 1991 after obtaining his PhD. He is currently an associate professor in the School of Mechanical and Aerospace Engineering, NTU. He served as the Head of Engineering Mechanics Division in the school from 2008 to 2011. Du’s research interests are mainly in three areas: 1) numerical and computational methods for engineering applications; 2) MEMS and microfluidics; 3) smart materials and their engineering applications. He has obtained over 10 research grants from various research funding agencies in the past, totaling more than S$10 million as PI/Co-PI/collaborator. He has published over 200 international refereed journal papers, more than 100 conference papers and a few invited book chapters. His journal papers were cited over 4800 times in the SCI with a H-Index of 38.
66 2018 International Conference of Biomedical Information Perception & Microsystem Manipulation of Microparticles Into 3D Matrix Patterns Using Standing Surface Acoustic Waves Microfluidics
Hejun Du*, Tan-Dai Nguyen and Van-Thai Tran School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore *Email: [email protected]
Abstract: Manipulation of microparticles or living cells in microfluidics has potential applications in biology, tissue engineering, microfluidics at micro-scale. Among many potential techniques, surface acoustic wave (SAW) has attracted much research attention due to its advantages such as non-invasive manipulation, low power consumption, capabilities of automatic process and unharmful handling process for living biological objects, such as single and group of cells. However, current studies show limitations in vertically controlling capability (i.e. larger than 100 µm) and precise manipulation of motions of microparticles in a large-scale chamber (e.g. millimeter-scale in depth). Hence, this paper aims to expand the capability of 3D manipulating of microparticles, and a new method was established with the help of an optical prism to simultaneously observe the vertical and horizontal 3D alignment and patterning of microparticles generated using standing surface acoustic waves. We discovered that the acoustic radiation force worked more effectively with large-size microparticles, with diameters from 6 µm to 20 µm, thus was more effective to form 3D lines or matrix patterns, whereas the dynamic movement of small-size microparticles (such as 1 µm) was dominated by acoustic streaming. The microparticles could be well-positioned at a specifically vertical location if there was a balance of forces including acoustic radiation force, drag force, buoyant force and gravity exerted onto the microparticles. Because the acoustic radiation force was increased gradually from the bottom to the top of the chamber, microparticles could be levitated to a higher position up to 1 mm by simply increasing the power. A numerical study was conducted to understand its principle, and the results matched well with the experimentally observed motions of the microparticles.
FIG. 2. A single 20 µm-microparticle was trapped at 3D nodes by switching on the input power. The multimedia view is a demonstration video. (Multimedia view) [URL:]
References Y. Q. Fu, J. K. Luo, N. T. Nguyen, A. J. Walton, A. J. Flewitt, X. T. Zu, Y. Li, G. McHale, A. Matthews, E. Iborra et al., Progress in Materials Science 89, 31 (2017). James Friend and Leslie Y. Yeo, Reviews of Modern Physics 83 (2), 647 (2011).
67 2018 International Conference of Biomedical Information Perception & Microsystem C.02
Guoqing Hu Professor Instiute of Mechanics Chinese Academy of Sciences
Biography
Guoqing Hu is currently a full professor in the Institute of Mechanics and University of Chinese Academy of Sciences. He has published more than 60 SCI journal papers. His research interest includes microfluidics, nanofluidics, lab-on-a-chip, and nanotoxicology. Dr. Hu also serves as the deputy director of the State Key Laboratory of Nonlinear Mechanics, the deputy secretary general of the Chinese Society of Theoretical and Applied Mechanics, and the editor of Scientific Reports and Acta Mechanica Sinica.
68 2018 International Conference of Biomedical Information Perception & Microsystem Inertial and viscoelastic effects for micro/nano-particles manipulation Guoqing Hu1,2 1State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China 2 School of Engineering Science, University of Chinese Academy of Sciences, Beijing 100149, China *Email: [email protected]
Precise manipulation of micro- and nanoparticles, such as cells, bacteria, synthetic nanoparticles and biomacromolecules, is important for a wide range of applications in biology, medicine, material and environment. Driven by inertial and elastic forces, particles suspended in microchannel flow can migrate across streamlines, resulting in the focusing, enrichment and separation of particles with various sizes. We carried out a systematic study of this key problem using numerical simulation, experiments, and analysis. A semi-analytical model was proposed for efficiently predicting the particle migration in inertial microfluidic devices. Different microfluidic devices were designed to realize the separation of microscale/nanoscale particles, red blood cells, bacteria, circulating tumor cells, and exosomes.
(a) (b)
Fig. 1 (a) Inertial particle focusing pattern depending on aspect ratio, blockage ratio, and Reynolds number. (b) Focusing and separation nanoparticles using elastic effects in microchannels.
1. Liu C, et al. Inertial focusing of spherical particles in rectangular microchannels over a wide range of Reynolds numbers. Lab Chip, 2015, 15: 1168-1177. 2. Liu C, et al. Sheathless focusing and separation of diverse nanoparticles in viscoelastic solutions with minimized shear thinning. Anal Chem, 2016, 88: 12547-12553. 3. Liu C, et al. Field-free isolation of exosomes from extracellular vesicles by microfluidic viscoelastic flows. ACS Nano, 2017, 11: 6968-6976.
69 2018 International Conference of Biomedical Information Perception & Microsystem C.03
Shiyang Tang Vice-Chancellor's Post-doctoral Research Fellow School of Mechanical, Materials, Mechatronic and Biomedical Engineering, University of Wollongong Wollongong, NSW 2522, Australia
Biography
Dr. Tang obtained his BEng and PhD from RMIT University, Australia. He later conducted his postdoctoral research in USA at Pennsylvania State University, and the University of California at San Francisco (UCSF). He is currently a Vice-Chancellor's Post-doctoral Research Fellow with School of Mechanical, Materials, Mechatronic and Biomedical Engineering University of Wollongong. He has established a strong track record of research and achievement in the fields of microlfuidics and liquid metal enabled devices, and published 42 papers in high-impact journals such as PNAS, Advanced Materials, Advanced Functional Materials, Small, Lab on a Chip, and Analytical Chemistry etc. His research interests cover mainly microfluidics, liquid metal enabled platfroms, and smart materils based lab-on-a-chip systems.
70 2018 International Conference of Biomedical Information Perception & Microsystem Microfluidic Mass Production of Stabilized and Stealthy Liquid Metal Nanoparticles Shi-Yang Tang1*, Weihua Li1 School of Mechanical, Materials, Mechatronic and Biomedical Engineering University of Wollongong Wollongong, NSW 2522, Australia *Email: [email protected]
Functional nanoparticles comprised of liquid metals, such as eutectic gallium indium (EGaIn) and Galinstan, present exciting opportunities in the fields of flexible electronics, sensors, catalysts, and drug delivery systems [1, 2]. Methods used currently for producing liquid metal nanoparticles have significant disadvantages as they rely on both bulky and expensive high-power sonication probe systems, and also generally require the use of small molecules bearing thiol groups to stabilize the nanoparticles. Herein, an innovative microfluidics-enabled platform is described as an inexpensive, easily accessible method for the on-chip mass production of EGaIn nanoparticles with tunable size distributions in an aqueous medium [3]. A novel nanoparticle stabilization approach is reported using brushed polyethylene glycol chains with trithiocarbonate end-groups negating the requirements for thiol additives while imparting a “stealth” surface layer. Furthermore, a surface modification of the nanoparticles is demonstrated using galvanic replacement and conjugation with antibodies. It is envisioned that the demonstrated microfluidic technique can be used as an economic and versatile platform for the rapid production of liquid metal-based nanoparticles for a range of biomedical applications.
Reference [1] Khoshmanesh K, Tang S-Y, et al. Liquid metal enabled microfluidics. Lab Chip 2017, 6: 974-993. [2] Daeneke T et al. Liquid metals: fundamentals and applications in chemistry. Chem. Soc. Rev. 2018, DOI: 10.1039/C7CS00043J [3] Tang S-Y, et al. Microfluidic mass production of stabilized and stealthy liquid metal nanoparticles. Small 2018, DOI: 10.1002/smll.201800118
71 2018 International Conference of Biomedical Information Perception & Microsystem C.04
Fei Duan Associate Professor and Assistant Chair (academic) School of Mechanical and Aerospace Engineering Nanyang technological University
Biography hoto
Dr. Fei Duan joined in Nanyang technological University (NTU), Singapore in July 2008 after three-year postdoc study in University of Toronto in Canada, now he works there as a tenured associate professor and assistant chair (academic) in School of Mechanical and Aerospace Engineering at NTU. He graduated with his Ph.D. degree from University of Toronto, Canada in 2005. He also worked as a visiting scientist in Institute of Fluid Mechanics at Friedrich-Alexander-University, Erlangen-Nuremberg, Germany. The topics of his researches covers droplet wetting and evaporation dynamics, Marangoni flow, particle self-assembly in droplet drying, enhanced thermal cooling for industrial applications and data center management, etc. Dr. Duan has advised 14 Ph.D. and 7 Master’s research students. Dr. Duan has published about 190 journal papers, conference papers and book chapters, among them, over 100 are the peer reviewed journal papers. He is a member of ASME, ACS and APS.
72 2018 International Conference of Biomedical Information Perception & Microsystem Controlled Wetting and Evaporation Transition in a Drying Sessile Droplet Fei Duan1,*, Xin Zhong1, Junheng Ren1 1School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore *Email: [email protected]
Droplet evaporation and wetting are widely used in the biomedical application and advanced manufacture [1]. Controlling the depositions in the desired configurations has attracted more attention. For the circular wetting on the smooth homogeneous surfaces of the nanofluid droplet [2], the branched structure was formed in the sessile nanofluid droplet with the receding contact angle. The coffee ring to uniform deposition can be controlled by adding the surfactants. The models have been developed with the kinetic monte carlo model for the fingered structures and the diffusion limited cluster aggregation model for the transition from the coffee ring to the uniform deposition. In addition, the non-circular depositions have been investigated as well. The surfactant solutions were prepared by dissolving cetyltrimethylammonium bromide powders in the nano-filtered water. The substrate for sessile droplets was the poly (methyl methacrylate) ones patterned with micropyramid islands using nanoimprint lithography. The drying processes of the droplets were simultaneously captured from a top view and two high-speed cameras from two side views. The contact angle and wetting diameters were measured. The droplets were evaporating in the open static atmosphere. The distinct spontaneous wetting transitions have been shown in the surfactant solution droplets drying on the patterned surface. As seen in Figure 1, the droplet keeps an octagonal shape to the end of drying under low initial surfactant concentrations. Under intermediate initial surfactant concentrations, the initial octagon spreads to a square firstly, then evolves to a stretched rectangle. The droplet mainly exhibits the octagon-to-square transition under the high initial surfactant concentrations. The wetting transitions which are induced by the varous concentration of an evaporating droplet can lead to an evolving shape of the droplet on the patterned surface. It might shed lights on the liquid pattern control.
Figure 1: Drying sequences of the droplets as theinitial surfactant concentration is (a) 0.00 mM, (b) 0.08 mM and (c) 0.40 mM, (d) 0.80 mM. [1] Ziauddin J, Sabatini DM. Microarrays of Cells Expressing Defined cDNAs. Nature 2001, 411: 107-110. [2] Crivoi A, Zhong X, Duan F. Crossover from the Coffee-Ring Effect to the Uniform Deposit Caused by Irreversible Cluster-Cluster Aggregation. Phys. Rev. E 92 (3): 032302.
73 2018 International Conference of Biomedical Information Perception & Microsystem C.05
Peng Xue Associate Professor Institute of Clean Energy and Advanced Materials Southwest University
Biography
Dr. Peng Xue accomplished his undergraduate study in Harbin Institute of Technology, China in 2009, and received his Ph.D in bioengineering from Nanyang Technological University (NTU), Singapore in 2015. Afterwards, he continued his research at NTU as a postdoc research fellow. Currently, he is appointed as the associated professor in Institute for Clean Energy and Advanced Materials, Southwest University, China. His research interests include the design of wearable microdevices for drug delivery or sensing purpose, as well as BioMEMs systems. He has published more than 40 peer reviewed research articles and filed five patents.
74 2018 International Conference of Biomedical Information Perception & Microsystem Surface Modification of Polydimethylsiloxane (PDMS) to Enhance Hemocompatibility for Potential Applications in Medical Implants or Devices Qian Li1,2, Yuan Li3, Lihong Sun1,2, Lei Zhang4, Zhigang Xu1,2, Yuejun Kang*,1,2, Peng Xue*,1,2 1 Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China. 2 Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices, Chongqing 400715, China. 3 Affiliated Yongchuan Hospital of Chongqing Medical University, Chongqing 402160, China. 4 State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. * E-mail: [email protected]. Tel: +86-23-68254056. Fax: +86-23-68254969.
Polydimethylsiloxane (PDMS) have been popularly applied in microelectromechanical systems (MEMS) and implantable devices. To improve the hemocompatibility of PDMS surface for enhanced applicability of PDMS-based implant, a facile technique was developed by modifying PDMS with hyaluronic acid (HA) and polydopamine (PDA) complex (HA-PDA). Platelet adhesion and activation was considerably reduced on PDMS substrates modified with HA-PDA, indicating an enhanced hemocompatibility compared to native PDMS or those coated with HA or PDA solely. HA-PDA coating also exhibited minimal cytotoxicity by evaluating the adhesion and proliferation of endothelial cells (HUVECs) on modified PDMS surface. Finally, the anti-inflammation effect of PDMS surface modified with HA-PDA was demonstrated by measuring the amount of released chemokines. To obtain the combinatorial effects of hemocompatibility, angiogenesis and anti-inflammation property, the optimal amount of HA-coating was screened and determined for PDMS modification. These findings demonstrated that HA-PDA modified PDMS has an exceptional potential to serve as the core or packaging material for constructing implantable device in clinical applications.
References 1. Menon, N. V.; Chuah, Y. J.; Phey, S.; Zhang, Y.; Wu, Y.; Chan, V.; Kang, Y. Microfluidic Assay To Study the Combinatorial Impact of Substrate Properties on Mesenchymal Stem Cell Migration. ACS Appl. Mater. Interfaces 2015, 7: 17095−17103. 2. Du, X.; Li, L. X.; Li, J. S.; Yang, C. W.; Frenkel, N.; Welle, A.; Heissler, S.; Nefedov, A.; Grunze, M.; Levkin, P. A., UV-Triggered Dopamine Polymerization: Control of Polymerization, Surface Coating, and Photopatterning. Adv. Mater. 2014, 26 : 8029-8033.
75 2018 International Conference of Biomedical Information Perception & Microsystem C.06
Qianbin Zhao Ph.D. candidate School of Mechanical, Materials, Mechatronic and biomedical Engineering University of Wollongong
Biography
Qianbin Zhao received his Bachelor degree in Engineering from the Northeastern University (NEU), Shenyang, China, in 2015. He recently holds a position as a Ph.D. candidate in the School of Mechanical, Materials, Mechatronic and Biomedical Engineering at University of Wollongong. His current research focuses on (i) inertial microfluidics for microparticle manipulation through geometry-induced secondary flow and (ii) Organ-on-a-chip
76 2018 International Conference of Biomedical Information Perception & Microsystem Inertial microparticle manipulation by sheath flow-enhanced secondary flow in slanted groove microchannel Qianbin Zhao1, Dan Yuan1, Shiyang Tang1, Sheng Yan2, Jun Zhang3*, Weihua Li1* 1 School of Mechanical, Materials, Mechatronic and biomedical Engineering, University of Wollongong, Wollongong, NSW 2522, Australia, Fax: +61 2 4221 3238; Tel: +61 2 4221 3490; E-mail: [email protected] 2 Department of Physics, The Hong Kong University of Science and Technology, Hong Kong, China 3 Queensland Micro and Nanotechnology Centre, Griffith University, Brisbane, QLD 4111, Australia
In the regime of inertial microfluidics, secondary flow is widely adopted to alter and optimize the equilibrium position and focusing performance of microparticles. In this work, we proposed a double-layer microchannel with slanted groove structures to generate continuous transverse secondary flow for the microparticle manipulation. Moreover, a sheath flow was introduced to enhance the performance of microparticle manipulation by the structure-generated secondary flow, especially for the relatively small size microparticle. The effects of flow rate and ratio between the sheath and sample flow were investigated exhaustively within a large range (the ratio was from 1 to 7 and the flow rate was from 200 to 700μl/min). The captured microparticle fluorescent trajectories demonstrated that the 4.8μm microparticle could be focus or manipulate effectively at different flow rate and ratio conditions. And larger 9.9 and smaller 2.9μm microparticles could be also guided to the equilibrium position as well at corresponding different modified flow rate and ratio. Meanwhile, a plasma extraction with the undiluted whole blood was conducted in this microchannel to prove the practical functionality of this method. The results achieved show that the purity of plasma extracted could reach up to ~99% in a single process validated by the flow cytometry and hemocytometer, when the ratio of sheath flow and sample flow applied was ~6 and the total flow rate was ~700μl/min. In conclusion, the sheath flow-enhanced secondary flow microparticle manipulation method offers stronger manipulation ability of smaller-size or higher-concentration microparticle comparing with the conventional inertial microfluidics method, which is great potential in the biological and diagnostic assays.
Zhao QB, et al. High-throughput sheathless and three-dimensional microparticle focusing using a microchannel with arc-shaped groove arrays. Scientific reports. 2017, 7:41153. Zhao QB, et al. Zhao Q, Yuan D, Yan S, et al. Flow rate-insensitive microparticle separation and filtration using a microchannel with arc-shaped groove arrays. Microfluidics and Nanofluidics. 2017, 21(3):55.
77 2018 International Conference of Biomedical Information Perception & Microsystem C.07
Ye TIAN Ph.D. Department of Mechanical Engineering The University of Hong Kong
Biography
Ye TIAN received BEng degree in Process Equipment & Control Engineering from the Jiangnan University in 2010, MS degree in Mechanical Engineering from Stevens Institute of Technology in 2013 and Ph.D. degree from Department of Mechanical Engineering, the University of Hong Kong in 2018. His research interests cover Microfluidics, Biomaterials Tissue Engineering and Functional Surface and Interfaces.
Bioinspired Cavity-Microfibers from Microfluidics
We fabricate unique microfibers with spindle cavity-knots that mimic the morphology of spider silk from composite hydrogels (named cavity-microfiber) by simple gas-in-water microfluidics. The cavity-microfiber is endowed with unique surface roughness, mechanical strength, and long-term durability. The cavity-microfiber can be used for water collection. The maximum water volume collected on a single knot is almost 495 times than that of the knot. Furthermore, the cavity-microfibers are assembled controllably into spider-web-like networks for large-scale water collection. The cavity-microfibers are also potential candidates for tissue engineering, encapsulation and controlled release, and controlled liquid transport.
78 2018 International Conference of Biomedical Information Perception & Microsystem Bioinspired Cavity-Microfibers from Microfluidics Ye Tian1, 2, Liqiu Wang1, 2* 1Department of Mechanical Engineering, the University of Hong Kong, Hong Kong 2 HKU-Zhejiang Institute of Research and Innovation (HKU-ZIRI), Hangzhou, Zhejiang, 311300, China *Email: [email protected]
Microfibers are of increasing interest because they have a broad range of applications in diverse fields, including biomedical engineering, tissue engineering, information technology, biomaterials and sensor technologies. In applications, properties and functions of microfibers are highly dependent on their morphologies. Microfluidics enables good controllability of microscale jets and droplets, and is thus capable of producing microfibers with precisely-tuned morphologies. Here we fabricate unique microfibers with spindle cavity-knots that mimic the morphology of spider silk from composite hydrogels (named cavity-microfiber) by simple gas-in-water microfluidics. The cavity-microfibers are templated from jet phase with gas bubbles via cross-linking and drying. The cavity-microfiber is endowed with unique surface roughness, mechanical strength, and long-term durability due to the design of cavity as well as polymer composition, thus enabling an outstanding performance in applications. The fiber diameter, the knot size and the distance between knots can be conveniently controlled by the flow rates of the jet phase and the pressure of the gas phase, respectively. The production rate of cavity-microfibers is also highly dependent on the flow rate of jet phase. Through tuning the flow rate of jet phase, the simple microfluidic approach enables the generation of such cavity-microfiber in large quantities with high-throughput. Inspired by spider silk collecting water from humid air witnessed by a large number of water droplets hanging on them in the early morning, the cavity-microfiber can be used for water collection. The maximum water volume collected on a single knot is almost 495 times than that of the knot. Due to the robustness of the cavity-microfiber, the cavity knots maintain their shape and functions for cycles of water-collecting. Furthermore, the cavity-microfibers are assembled controllably into spider-web-like networks for large-scale water collection. The result indicates the water collection is even more efficient and scalable with spider-web-like networks. These light-weighted yet tough, low-cost bioinspired cavity-microfibers offer promising opportunities for large-scale water collection in water-deficient areas and are potential candidates for tissue engineering, encapsulation and controlled release, and controlled liquid transport.
Fig.1. Microfluidic fabrication of bio-inspired cavity-microfiber for water collection REFERENCES: [1] Y. Tian, P. Zhu, X. Tang, C. Zhou, J. Wang, T. Kong, M. Xu and L. Wang, “Large-scale water collection of bioinspired cavity-microfibers,” Nat. Commun. 2017, 8: 1080.
79 2018 International Conference of Biomedical Information Perception & Microsystem C.08
Yi Zhang assistant professor School of Mechanical and Aerospace Engineering Nanyang Technological University
Biography
Yi Zhang is currently an assistant professor at the School of Mechanical and Aerospace Engineering at Nanyang Technological University, Singapore. He received his Ph.D in Biomedical Engineering from Johns Hopkins University School of Medicine, USA, in 2013 and B.Eng in Bioengineering from Nanyang Technological University, Singapore, in 2007. He received his postdoc training in the Institute of Bioengineering and Nanotechnology, the Agency of Science Technology and Research (A*STAR), Singapore from 2013−2015, and subsequently worked there as a Research Scientist from 2015−2016. His research aims to develop micro and nano systems to bridge the gap between engineering advancement and current medicine practice. Yi has published over 30 peer-reviewed papers with an H-index of 17. In recognition of his work, he has been awarded the Hodson Fellowship and the prestigious Siebel Scholarship in U.S.A. He is also the awardee of the Chinese Government Award for Outstanding Self-Financed Students Abroad by China Scholarship Council. He has received several best paper awards and art-in-science awards at various conferences.
80 2018 International Conference of Biomedical Information Perception & Microsystem Polydopamine-Modified Magnetic Digital Microfluidic Platform for Biosensing Yi Zhang School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798; Email: [email protected]; Tel: +65 67905590
Digital microfluidic platform controls fluid by manipulating droplets on an open surface.[1] The surface is modified with a hydrophobic coating, such as Teflon, to allow easy droplet movement. Nevertheless, to achieve a more comprehensive range of droplet manipulation, the hydrophobic surface is often patterned with hydrophilic features to assist droplet control. Because Teflon is nonwettable and chemically inert, modifying Teflon-coated surface is not straightforward. Inspired by mussel adhesive protein, polydopamine (PDA) is the polymerized form of catechol such as 3,4-dihydroxy-L-phenylalanine (DOPA) or dopamine PDA adheres strongly to almost all types of surfaces, which offers tremendous potential for any applications involving in surface modifications. With a simple contact of dopamine monomer solution on the substrate, polymerization process occurs spontaneously in an alkaline environment and form a dark brown layer of PDA adherent to the substrate. We have combined PDA-enabled surface modification with magnetic digital microfluidics to realize droplet mixing, separating, transferring and patterning on a hydrophobic/hydrophilic patterned Teflon surface. Various patterns of PDA have been coated onto the Teflon surface via a silicone stencil. Using this platform, we have demonstrated droplet moving, particle extraction, liquid dispensing, droplet cross-platform transfer, and liquid patterning, which would greatly extend the applicability of magnetic digital microfluidics.
a.)
b.)
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Fig. 1. Droplet manipulation with magnetic digital microfluidics on PDA-patterned Teflon substrate (a) Particle extraction. (b) Liquid Dispensing. (c) Transfer designated droplets from one glass slide to another. (c) Liquid patterning following PDA pattern.
Reference [1] Zhang, Y. and T.H. Wang, Full‐Range Magnetic Manipulation of Droplets via Surface Energy Traps Enables Complex Bioassays. Advanced Materials, 2013. 25(21): p. 2903-2908. [2] Zhang, Y., Magnetic Digital Microfluidics-A Review, Lab on a Chip, 2017, 17, 994-1008
81 2018 International Conference of Biomedical Information Perception & Microsystem C.09
Fei Liu Professor School of Ophthalmology & Optometry, School of Biomedical Engineering Eye Hospital (Zhejiang Eye Hospital) Wenzhou Medical University
Biography
Fei Liu received his Ph.D. in Chemical and Biomolecular Engineering from the Korea Advanced Institute of Science and Technology (KAIST) in 2012. He is currently a professor with School of Ophthalmology & Optometry and School of Biomedical Engineering, Wenzhou Medical University. With a focus on nanomaterial, nanotechnology, biotechnology, and microfluidics for optical and electrical biosensor applications, he is interested in developing integrated platforms for biomedical diagnostic and clinical translation of extracellular vesicles (EVs). Together with colleagues, Liu has developed exosome-total-isolation-chip (ExoTIC) and EV hunter devices for simple and high-throughput isolation of EVs from different biological samples. He also demonstrated a nanoplasmonic method for quantification of EVs from plasma samples for diagnosis and treatment monitoring of pancreatic cancer. Dr. Liu has authored and co-authored over twenty papers, including Nature Biomedical Engineering, ACS Nano, Advanced Materials, Advanced Functional Materials, Small, etc. His publications have been cited more than 1900 times (h-index 17).
82 2018 International Conference of Biomedical Information Perception & Microsystem Isolation and Quantitative Analysis of Extracellular Vesicles for Cancer Early Detection Zaian Deng1 and Fei Liu1,2* 1Laboratory of Biomedical Diagnostics and Clinical Translation, School of Ophthalmology & Optometry, School of Biomedical Engineering, Eye Hospital (Zhejiang Eye Hospital), Wenzhou Medical University, Wenzhou, Zhejiang 325035, China 2Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, Zhejiang 325001, China *Email: [email protected]
Circulating tumor-derived extracellular vesicles (EVs) have emerged as a promising source for identifying novel cancer biomarkers for early cancer detection. However, translating tumor EVs into clinical diagnostics has been challenging due to the lack of reliable isolation methods and specific EV biomarkers. Here, we report two EV isolation methods called ExoTIC (exosome total isolation chip) and EV hunter which are simple, easy-to-use, modular and facilitates high-yield, and high-purity EV isolation from different biofluids (culture medium, plasma, and urine). Then, we report a rapid, ultrasensitive and inexpensive nanoplasmon-enhanced scattering (nPES) assay that directly quantifies tumor-derived EVs from as little as 1 µL of plasma. We identified a pancreatic cancer EV biomarker, ephrin type-A receptor 2 (EphA2), and demonstrate that an nPES assay for EphA2-EVs distinguishes pancreatic cancer patients from pancreatitis patients and healthy subjects. These technologies have the potential to enable accelerated EV-based biomarker discovery and small molecular analysis that is simple, reliable, and quantitative with broad applicability to diagnosis, prognostication, and treatment monitoring in patients with cancer.
References: 1. Liu F, et al. The Exosome Total Isolation Chip. ACS Nano 2017, 11:10712-10723. 2. Liang K, and Liu F, et al. Nanoplasmonic Quantification of Tumor-derived Extracellular Vesicles in Plasma Microsamples. Nat. Biomed. Eng. 2017, 1: 0021.
83 2018 International Conference of Biomedical Information Perception & Microsystem C.10
Jun Zhang Associate Professor School of Mechanical Engineering, Nanjing University of Science and Technology,
Biography
Jun Zhang received his bachelor degree in Engineering from the Nanjing University of Science and Technology (NJUST), Nanjing, China in 2009 with an Outstanding Graduate award and received a PhD degree in Mechanical Engineering from University of Wollongong, Australia in 2015. He has published more than 40 journal and conference articles. His research interests include but not limited to: 1) explore novel techniques for micro/nano-fluidic fabrication; 2) investigate the fundamental mechanism of inertial focusing and expand its application on bio-particle filtration, separation/sorting and ordering for bio-sample preparation and disease diagnosis; 3) develop hybrid real-time controllable inertial microfluidic systems for flexible bio-particle manipulation.
84 2018 International Conference of Biomedical Information Perception & Microsystem A novel single-layer microchannel for continuous sheathless single-stream particle inertial focusing Jun Zhang†*1, Yan Zhang†2, Fei Tang2, Weihua Li3, Xiaohao Wang2 1School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China 2 State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China. 3 School of Mechanical, Materials and Mechatronic Engineering, University of Wollongong, Wollongong, NSW 2522, Australia *Email: [email protected]; † Equally contributed Abstract High-throughput, high-precision single-stream focusing of microparticles has potential wide range of applications in bio-chemical analysis and clinical diagnosis [1, 2]. In this work, we develop a sheathless three-dimensional (3D) particle focusing method in a single-layer microchannel. This novel microchannel consists of periodic high-aspect-ratio curved channels and straight channels. The proposed method takes advantage of both the curved channels, which induce Dean flow to promote particle migration, and straight channels, which suppress the remaining stirring effects of Dean flow to stabilize the achieved particle focusing. The effects of flow rate, particle size, and cycle number on the focusing performance were investigated. The experimental results demonstrate that polystyrene particles with diameters of 5–20 μm can be focused into a 3D single file within 7 channel cycles with the focusing accuracy up to 98.5%. The focusing throughput could reach up to ~105 counts/min. Furthermore, its applicability to biological cells is also demonstrated by 3D focusing of HeLa and melanoma cells and bovine blood cells in the proposed microchannel. The proposed sheathless passive focusing scheme, featuring a simple channel structure, compact layout and uncomplicated fabrication procedure, holds great promise as an efficient 3D focusing unit for the development of on-chip flow cytometry.
Figure 1. (a) Particle 3D inertial focusing in a microchannel consisting of periodic straight and curved channel sections (MPSC microchannel). (1) Top-down view of particle distribution in the microchannel: (left) stacked BF image and (right) corresponding fluorescence image. (2) Confocal microscopy scanning of cross-sectional distribution of microparticles at the center of seven straight channel sections. (3) 3D trajectory of microparticles at the end of the seventh straight channel section.(b) Fluorescence image of particle distribution in straight channels; the green arrow is the sampling line for fluorescence profiles. (c) Fluorescence profiles of trajectories of different sized microparticles sampled at 7th straight cycles; Referemces: [1] A. J. Chung, D. R. Gossett, and C. D. Di, "Three dimensional, sheathless, and high-throughput microparticle inertial focusing through geometry-induced secondary flows," Small, vol. 9, p. 685, 2013. [2] S. Choi, S. Song, C. Choi, and J. K. Park, "Sheathless focusing of microbeads and blood cells based on hydrophoresis," Small, vol. 4, pp. 634-641, 2008.
85 2018 International Conference of Biomedical Information Perception & Microsystem C.11
Xinyi Guo Ph.D. Student Department of Precision Instrument and Opto-electronics Engineering Tianjin University
Biography
Xinyi Guo received her B.E. degree in Measuring and Control Technology and Instrumentations from Tianjin University in 2015, and is currently a Ph.D. student from School of Precision Instrument and Opto-electronics Engineering, Tianjin University. Her research interests cover mainly bioMEMS, gigahertz ultrasound, cell poration, drug delivery, biosensors and bioelectronics.
Cell membrane poration towards intracellular delivery based on gigahertz electromechanical resonators
Efficient intracellular delivery of exogenous materials remains a critical issue in fundamental biological researches and clinical applications. Here, we developed a novel chemical-free method for intracellular delivery enhancement using a designed gigahertz ultrasonic electromechanical resonator. When excited by a sinusoidal electric signal, the propagation and attenuation of acoustic wave in liquid will generate high-speed acoustic streaming. The liquid above the device working area will be accelerated and strike the substrate surface, thus generates pressure on cells, induces deformation and membrane poration, and finally realizes delivery of exogenous materials. To verify the intracellular delivery ability, DOX was selected as an example, and an enhanced fluorescence of DOX in cells exposed to resonator stimulation can be seen. We also realized the delivery of fluorescent-labeled DNA strains and plasmids. Besides, different power applied to the resonator can induce different fluid velocity, thus generate different force intensity and control the deliver efficiency. Pores on membranes induced by acoustic streaming treatment were observed by SEM. Disrupted cell membranes and porous structures can be seen after treatment, and resealed after 10 min recovery, indicating a strong fluid force exerted on cells and the influence is temporary and reversible. All the results proved that the gigahertz resonator has provided a versatile, well-controlled and promising cell poration method for intracellular delivery.
86 2018 International Conference of Biomedical Information Perception & Microsystem Cell membrane poration towards intracellular delivery based on gigahertz electromechanical resonators Xinyi Guo1, Hongxiang Zhang2, Yanyan Wang1, Wei Pang2 and Xuexin Duan1* 1 State Key Laboratory of Precision Measuring Technology & Instruments, Tianjin University, Tianjin300072, 2 College of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300072, China * E-mail: [email protected] Efficient intracellular delivery of exogenous materials remains a critical issue in fundamental biological researches and clinical applications [1]. Here, we developed a novel chemical-free method for intracellular delivery enhancement using a designed gigahertz ultrasonic electromechanical resonator [2]. When excited by a sinusoidal electric signal, the propagation and attenuation of acoustic wave in liquid will generate high-speed acoustic streaming. The liquid above the device working area will be accelerated and strike the substrate surface, thus generates pressure on cells, induces deformation and membrane poration, and finally realizes delivery of exogenous materials. To verify the intracellular delivery ability, DOX was selected as an example, and an enhanced fluorescence of DOX in cells exposed to resonator stimulation can be seen. We also realized the delivery of fluorescent-labeled DNA strains and plasmids. Besides, different power applied to the resonator can induce different fluid velocity, thus generate different force intensity and control the deliver efficiency. Pores on membranes induced by acoustic streaming treatment were observed by SEM. Disrupted cell membranes and porous structures can be seen after treatment, and resealed after 10 min recovery, indicating a strong fluid force exerted on cells and the influence is temporary and reversible. All the results proved that the gigahertz resonator has provided a versatile, well-controlled and promising cell poration method for intracellular delivery.
Figure 1: (A) Schematic of the intracellular delivery system. (B) SEM picture of gigahertz acoustic device surface. (C) Device structure. (D) 2D FEM analysis of acoustic streaming under resonator stimuli and its velocity distribution. (E) Results of DOX delivery, in which enhanced nucleus uptake can be seen. (F) Different delivery efficiency under different power. (G) Pores on membranes induced by acoustic streaming treatment observed by SEM. References [1] Stewart MP, et al. In vitro and ex vivo strategies for intracellular delivery. Nature 2016, 538:183. [2] Zhang ZX, et al. Hypersonic Poration: A New Versatile Cell Poration Method to Enhance Cellular Uptake Using a Piezoelectric Nano‐Electromechanical Device. Small 2017, 13: 1602962.
87 2018 International Conference of Biomedical Information Perception & Microsystem C.12
Yang Yang PhD Student College of Precision Instrument and Optoelectronics Engineering Tianjin University
Biography
Yang Yang received B.S. degree in Measuring and Control Technology and Instrumentations from Chongqing University in 2015. He is currently a PhD candidate in College of Precision Instrument and Optoelectronics Engineering, Tianjin University. His research interests cover mainly microfluidics, acoustofluidics, ultrahigh frequency(UHF) resonator and cell manipulation.
Selective Trapping and Controllable Release of Single Cell via Three-Dimensional Acoustic Fluidics
Manipulation, trapping and retrieval of target biological specimens from blood samples are essential requirements for modern biological and medical researches. Lab-on-a-Chip system offers a simple, quick and portable solution. Recently, we demonstrated a versatile approach to generate three-dimensional (3-D) micro-vortices with ultrahigh frequency (UHF) resonators which has been successfully applied for fluid mixing. Here, we investigated an active approach to continuously isolate circulating tumour cells (CTCs) cells from human blood samples via such localized UHF acoustic fluidic device. UHF bulk acoustic wave resonators of 1.83 GHz were integrated into microfluidic channels. Stable and highly localized 3-D micro-vortices are generated that results from the nonlinear attenuation of oscillating displacements. By properly tuning the applied power and the flow rate, target cells with specific size are stably trapped within the vortices, while other blood cells are released. The trapped cells can be replaced by buffer, thus achieve the cell separation. After switching off the device, the trapped cells can be retrieved without damage. By dedicated tuning the applied power, the capture cell size can cover all types of blood cells from 5 µm (platelets) to 20 µm (Hela cells). The dynamic trapping process is analysed to prove the advantages of our strategy in accuracy and stability via finite element simulation. Furthermore, due to the CMOS compatibility, gold electrodes can be integrated on the same silicon substrate. Thus, the trapped cell can be accurately counted by impedance sensor.
88 2018 International Conference of Biomedical Information Perception & Microsystem Selective Trapping and Controllable Release of Single Cell via Three-Dimensional Acoustic Fluidics Yang Yang, Xuexin Duan* 1State Key Laboratory of Precision Measuring Technology & Instruments, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China *Email: [email protected]
Manipulation, trapping and retrieval of target biological specimens from blood samples are essential requirements for modern biological and medical researches. Lab-on-a-Chip system offers a simple, quick and portable solution. Recently, we demonstrated a versatile approach to generate three-dimensional (3-D) micro-vortices with ultrahigh frequency (UHF) resonators which has been successfully applied for fluid mixing [1]. Here, we investigated an active approach to continuously isolate circulating tumour cells (CTCs) cells from human blood samples via such localized UHF acoustic fluidic device. UHF bulk acoustic wave resonators of 1.83 GHz were integrated into microfluidic channels. Stable and highly localized 3-D micro-vortices are generated that results from the nonlinear attenuation of oscillating displacements [2,3]. By properly tuning the applied power and the flow rate, target cells with specific size are stably trapped within the vortices, while other blood cells are released. The trapped cells can be replaced by buffer, thus achieve the cell separation. After switching off the device, the trapped cells can be retrieved without damage. By dedicated tuning the applied power, the capture cell size can cover all types of blood cells from 5 µm (platelets) to 20 µm (Hela cells). The dynamic trapping process is analysed to prove the advantages of our strategy in accuracy and stability via finite element simulation. Furthermore, due to the CMOS compatibility, gold electrodes can be integrated on the same silicon substrate. Thus, the trapped cell can be accurately counted by impedance sensor.
Figure 3. (a)CTCs isolation from human blood sample. (b) The efficiency of CTCs isolation from blood cells. (c) Simulation results of particle separation. 15µm particles (blue) were trapped at the boundary of the device while 2 µm particles (red) exited the microchannel. (d) Single cancer cell analysis with the dye mixed by PI and Calcein-AM.
[1] Cui W, et al. Localized ultrahigh frequency acoustic fields induced micro-vortices for submilliseconds microfluidic mixing[J]. Applied Physics Letters. 2016, 109(25): 253503. [2] Collins D J, et al. Highly localized acoustic streaming and size-selective submicrometer particle concentration using high frequency microscale focused acoustic fields[J]. Analytical chemistry, 2016, 88(10): 5513-5522. [3] Ahmed D, et al. Rotational manipulation of single cells and organisms using acoustic waves[J]. Nature communications, 2016, 7: 11085.
89 2018 International Conference of Biomedical Information Perception & Microsystem
D. Biomedical Systems and Artificial Intelligence(AI) Forum Meeting
90 2018 International Conference of Biomedical Information Perception & Microsystem D.01
Feng Guo Assistant Professor Department of Intelligent Systems Engineering Indiana University Bloomington
Biography
Feng Guo is currently an assistant professor in Department of Intelligent Systems Engineering, School of Informatics, Computing and Engineering, Indiana University-Bloomington. He has received his Ph.D. in Engineering Science and Mechanics from Penn State in 2015 and conducted a postdoctoral training at Canary Center, School of Medicine, Stanford University. His research focuses on developing biomedical devices, sensors, and systems for emerging biomedical applications.
Presentation Title: Acoustofluidic Manipulation of Single Cells
Sound can be music to please the ear, however, the waves produced can be utilized as “Acoustic Tools” for the manipulation and treatment of cells. The acoustofluidic technology, combining sound waves with microfluidics, becomes a revolutionary way to dexterously and noninvasively handle biological cells. Firstly, this technique manipulates or treats cells using gentle mechanical vibrations. These vibrations create a pressure gradient in the medium to deal with cells yielding a contamination-free, contactless, and label-free operation. Secondly, acoustofluidics has minimal impact on cell viability and function. Thirdly, this technology offers additional advantages in ease of use, versatility, and portability due to the simple setup of acoustics. Here, we report a series of acoustic biomedical engineering tools for cell manipulation and 3D bio-fabrication to address the problems in the field of biomedicine including tissue engineering, cancer treatment, and drug screening.
91 2018 International Conference of Biomedical Information Perception & Microsystem Acoustofluidic Manipulation of Single Cells Feng Guo1* 1Department of Intelligent Systems Engineering, Indiana University, Bloomington, Indiana 47405, United States *Email: [email protected]
Sound can be music to please the ear, however, the waves produced can be utilized as “Acoustic Tools” for the manipulation and treatment of cells. The acoustofluidic technology, combining sound waves with microfluidics, becomes a revolutionary way to dexterously and noninvasively handle biological cells. Firstly, this technique manipulates or treats cells using gentle mechanical vibrations. These vibrations create a pressure gradient in the medium to deal with cells yielding a contamination-free, contactless, and label-free operation. Secondly, acoustofluidics has minimal impact on cell viability and function. Thirdly, this technology offers additional advantages in ease of use, versatility, and portability due to the simple setup of acoustics. Here, we report a series of acoustic biomedical engineering tools for cell manipulation and 3D bio-fabrication to address the problems in the field of biomedicine including tissue engineering, cancer treatment, and drug screening.
92 2018 International Conference of Biomedical Information Perception & Microsystem D.02
Chun-Xia Zhao Associate Professor Australian Research Council (ARC) Future Fellow Group Leader at Australian Institute for Bioengineering Nanotechnology at The University of Queensland, Australia.
Biography
Associate Professor Chun-Xia Zhao is an Australian Research Council (ARC) Future Fellow and Group Leader at Australian Institute for Bioengineering and Nanotechnology at The University of Queensland, Australia. She leads a research team with a focus on the development of micro and nanostructures based on bio-inspired engineering and microfluidics for controlled release and drug delivery. She has been focusing on innovative research as evidenced by her four patents. A/Prof Zhao’s research has attracted more than $3.5 M in research funding since 2011, including four Australian Research Council projects as the lead investigator, two national prestigious fellowship, eight UQ grants, as well as industry funding. She has been recognised for scientific excellence with a 2016 UQ Foundation Research Excellence Award. She has built extensive collaborations with scientists at top universities such as Harvard University, Cornell University, etc. She visited Harvard University as a Fellow of the School of Engineering and Applied Science. She also serves as the Editor-in-Chief, Editorial Board member for several journals.
93 2018 International Conference of Biomedical Information Perception & Microsystem
Engineered micro/nanostructures for drug delivery and controlled release Associate Professor Chun-Xia Zhao ARC Future Fellow and Group Leader Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, Australia, 4072. [email protected]
Abstract
Engineered micro and nanomaterials have attracted significant research interest during the past decades for various applications. Nature provides us with a wide range of sophisticated nanomachines that serve as a source of building blocks for various functional materials. For example, diatoms and marine sponges use self-assembled biomolecules (proteins or peptides) as templates to create hierarchical organic-inorganic architectures with precisely controlled structure, size, orientation and composition, which has been considered as a paradigm for biomolecule-controlled self-assembly of hierarchical materials. Mimicking these functions of biomolecules provide the opportunity to develop various functional materials for different applications. My lab has been focusing on the development of advanced micro and nanostructures based on bioinspired engineering and microfluidic technology. Patented technologies have been developed for making tailorable complex emulsions and core-shell nanocapsules for controlled release and drug delivery. In this talk, I will introduce how we design and fabricate different kinds of micro and nanomaterials based on emulsion templating, how we apply them for different applications, and how their physical properties affect their biological functions.
94 2018 International Conference of Biomedical Information Perception & Microsystem D.03
KanLiu Professor School of Life Science and Technology University of Electronic Science and Technology of China
Biography
Kan Liu received B.S. in Applied Physics from the Wuhan University in 2002, and Ph.D. in Condensed Matter Physics from Wuhan University in 2007. He is currently a professor in School of Life Science and Technology of University of Electronic Science and Technology of China. He has been selected Chinese Most Cited Researchers in Biomedical Engineering Field by Elsevier for 2014-2017. His research interests cover mainly microfluidic chip for chemical synthesis and biomedical diagnosis.
Presentation Title：
Highly efficient isolation and accurate in situ analysis of circulating tumor cells (CTCs) by a commercially available microfluidic device
CTCs can travel in body through blood circulation and have been regarded as the cellular origin of cancer metastasis. Thus, isolation and enumeration of CTCs from peripheral blood is of great value in monitoring tumor prognosis and guiding individualized treatment. A novel wedge-shaped microfluidic device for highly efficiently CTC isolation from peripheral blood based on multiple biophysical properties was demonstrated. Using this device, we realized CTCs’ isolation and identification with the average recovery rate of 93.7%± 3.2% in DMEM and 91.0% ± 3.0% in whole blood sample under optimized conditions. Importantly, the microfluidic chip exhibited the feasibility of detecting CTCs from different types of solid cancer. Meaningfully, our platform identified CTCs (7.30±7.29 per 2 ml) with a positive rate of 75% (30/40) in gastric cancer (GC) patients. The microfluidic chip provides a simple and efficient platform for CTCs detection in different types of cancer patients, and it holds the potential of clinically application in monitoring GC prognosis and guiding individualized treatment in the future.
95 2018 International Conference of Biomedical Information Perception & Microsystem A microvalves-chip based on PDMS for microfluidics Ting Chen2, Kan Liu 1,2* 1 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, R. P. China 2 School of Electronic and Electrical Engineering, Wuhan Textile University, Wuhan, 430020, P. R. China
*Email: [email protected]
Abstract This paper reported the design, fabrication, and characterization of a sandwich type of air-actuated micro-valve chip based on PDMS membrane. The fabrication method is very simple combining glass engraving technology with transfer bonding technology. The micro-valve chip could sustain liquid or air pressures of up to 200kpa with no burst failure. And a good cut-off function to the liquid has been realized by micro-valve when the liquid pressure lower than the air pressure. In addition, multiple parallel array of valves were integrated into one microfluidic chip to provide the function of manipulation for complex flows.
Fig.1 (a) Schematic design of micro-valve chip. (b) Working principle of the micro-valves chip. References 1. Araci, I.E. and S.R. Quake, Microfluidic very large scale integration (mVLSI) with integrated micromechanical valves. Lab Chip, 2012, 12(16): 2803-2806. 2. Oh, K.W. and C.H. Ahn, A review of microvalves. Journal of Micromechanics and Microengineering, 2006, 16(5): R13-R39. 3. Shaegh, S.A.M., et al., Plug-and-play microvalve and micropump for rapid integration with microfluidic chips. Microfluidics and Nanofluidics, 2015, 19(3): 557-564.
96 2018 International Conference of Biomedical Information Perception & Microsystem D.04
Chuan-Biao WEN Professor Department of College of medical information engineering Chengdu University of Traditional Chinese Medcine,.
Biography Photo (1970)The director of Digital Medical Research Institute of Chengdu University of Traditional Chi nese Medicine, and the academic leader of Discipline of Chinese Medicine Informatics which was focused on cultivating by The State Administration of traditional Chinese medicine, and the exec utive vice president of medical information engineering college, the Master Instructor,and the pres ident of cloud health branch of Chinese medicine informatization research team. I mainly engaged in Chinese medicine informatics research and practice, Chinese medicine clinical digital system, I nternet of Things and Tracing to Traditional Chinese Medicine,Syndromes and biological informat ion,Chinese medicine cloud health, Big data, Artificial intelligence of TCM. I have undertaken mo re than 50 scientific research projects, including nine national issues , Provincial and ministerial le vel project 25 items. Such as ,Study on biological technology platform of TCM syndrome of viral hepatitis", Key technologies of digitalization of traditional Chinese Medicineetc..I have developed more than 30 network platforms and software products, such as ,HIS system of primary medical i nstitutions in Chinese medicine", “Sichuan provincial community health information training prog ram", “Data network platform for inheriting clinical experience of famous TCM doctors", “Tracea bility system of genuine medicinal materials", "National medicine information collection platform " etc..
97 2018 International Conference of Biomedical Information Perception & Microsystem Research on intelligent algorithm model of TCM syndrome differentiation and treatment based on TCM Chuanbiao Wen Abstract text: The subject studied the traditional and modern SDAT system in TCM, started from the syndrome differentiation of four aspects (the cause, location, characteristics and conditions of the disease), constructed a quantitative model of TCM SDAT regarding the cause, location, characteristics, and conditions of the disease, collected the symptom information on the diagnosed subject and transferred them to the SDAT assistant algorithm for calculation and analysis, to determine the cause, location, characteristics and conditions of the disease, diagnosis and infer the potential syndromes of the diagnosed subject. Therapeutic method was recommended from the knowledge base according to the differentiation result, with the prescription and traditional Chinese medicines recommended according to the therapeutic method. This model realized a computable SDAT, and provided a SDAT diagnostic protocol supporting in integration of theory, method, prescription, and medicine (in integration of symptom-syndrome-prescription -medicine), to specify and assist in the differentiation diagnosis and treatment processes of TCM, generate the EMR with TCM characteristics and improve the service quality of TCM diagnosis and treatment. At the same time, the algorithm optimization was discussed, which provided a further work program in promoting the coincidence rate of the algorithm.
98 2018 International Conference of Biomedical Information Perception & Microsystem D.05
Yu, Ling Professor Faculty of Materials and Energy Southwest University
Biography hoto
Ling Yu (MD, PhD) is currently professor of the Faculty of Materials and Energy in Southwest University, China. She obtained her PhD degree in Bioengineering from Nanyang Technological University, Singapore in 2009. She conducted 3 years post-doctoral research at University of Pittsburgh Medical Center. Her research aims at exploring the advanced nanotechnologies and lab on chip to biomedical applications. She has published more than 40 journal papers, 1 book chapter and holds 12 patens.
“do-it-yourself”: Fast prototyping and fabrication of micro devices in a non-cleanroom setting
Researchers are interested in utilizing micro-devices to investigate their personalized samples, targets, and systems. Versatile micro-devices have been fabricated by using Parafilm® film, a universal laboratory cohesive thermoplastic. First, shaped thermoplastic film was laminated with transparent thin poly(ethylene terephthalate) film to build two- and three-dimensional microfluidic chips. Next, the cohesive thermoplastic assisted patterning was successfully used to fabricate 2D and 3D electrodes for biosensing application. The shaped-Parafilm® film was further explored as a template to pattern protein and cell adhesion and to form hydrophobic barrier to fabricate paper-based microfluidic analytical devices. The flexibility, convenience and operability seen here make those Parafilm® film-based methods a fast prototype strategy to evaluate the proof-of-concept design. It also provides alternative fabrication solutions for researchers from resource-limited laboratories to work on miniaturized devices.
99 2018 International Conference of Biomedical Information Perception & Microsystem “do-it-yourself”: Fast prototyping and fabrication of micro devices in a non-cleanroom setting Yao Lu, ZhuanZhuan Shi, Ling Yu* Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, P.R. China *E-mail: [email protected] Abstract Doing more with less is the slogan of Lab-on-a-Chip. More and more researchers are interested in utilizing micro-devices to investigate their personalized samples, targets, and systems. Taking a “do-it-yourself” approach to fabricating micro-devices of desired shapes creates a microenvironment that provides new ways to study subjects of research interest. Obviously, a cost-effective, high-throughput micro device fabrication protocol, which is readily accessible to universal laboratories, would be highly appreciated. To this aim, Parafilm® film, a universal laboratory cohesive thermoplastic, has been shaped and laminated with transparent thin poly(ethylene terephthalate) film to build versatile two- and three-dimensional microfluidic chips. Moreover, the cohesive thermoplastic assisted patterning was successfully used to fabricate versatile 2D and 3D electrodes for biosensing application. Other than work on a 2D flat substrate, the cohesive thermoplastic origami can easily fabricate electrodes on 3D objects, demonstrating itself as a good candidate to implant electronic unit on a stereotype object. The shaped-Parafilm® film was further explored as a template guiding protein and cell patterning and forming hydrophobic barrier to fabricate paper-based microfluidic analytical devices. The flexibility, convenience and operability seen here make those Parafilm® film-based methods a fast prototype strategy to evaluate the proof-of-concept design. It also provides alternative fabrication solutions for researchers from resource-limited laboratories to work on miniaturized devices.
References 1. A. A. Kumar, J. W. Hennek, B. S. Smith, S. Kumar, P. Beattie, S. Jain, J. P. Rolland, T. P. Stossel, C. Chunda-Liyoka and G. M. Whitesides, From the Bench to the Field in Low-Cost Diagnostics: Two Case Studies, Angew Chem Int Edit, 2015, 54, 5835-5852. 2. X. Li, D. R. Ballerini and W. Shen, A perspective on paper-based microfluidics: Current status and future trends, Biomicrofluidics, 2012, 6, 11301-1130113. 3. Y. Lu, Z.Z. Shi, L. Yu, C.M. Li Fast Prototyping of a Customized Microfluidic Device in a non-clean-room Setting by Cutting and Laminating Parafilm®. RSC Adv, 2016, 6, 85468 4. Z.Z. Shi, X.S. Wu, Y. Lu, Z.S. Lu, C.M. Li*, L.Yu* Fast and low-cost patterning of electrodes on versatile 2D and 3D substrates by cutting and origami cohesive thermoplastic for biosensing applications, Sensor Actuat B-Chem, 2018, 255, 2431-2436
100 2018 International Conference of Biomedical Information Perception & Microsystem D.06
Jin Yang Professor Sensors and Instruments Research Center College of Optoelectronic Engineering
Biography
Jin Yang received the BE, ME and PhD degrees in instrumentation science and technology from Chongqing University in 2002, 2004, and 2007, respectively. Currently, he is a professor with the College of Optoelectronic Engineering, Chongqing University. His current research interests focus on sensor and actuator, measurement and instrumentation, nanogenerator, self-powered sensor and systems.
TENG based pressure sensor for continuous measurement of human arterial pulse wave
In this work, we developed a TENG based pressure sensor for capturing subtle mechanical change of the blood pressure in the vessel and expressing it in electrical signals as human pulse waveform. Our TENG based pressure sensor holds a low pressure detection limit of 5 Pa and a small scale of 10×10×1 cubic millimeters for ease in carrying. It is capable of continuous measurement of human pulse wave. In addition, based on the TENG based pressure sensor, a low power consumption sensor system was further developed, including a TENG based pressure sensor for human pulse signal extraction, a management circuit for signal processing and a wireless transmission component to communicate the measured cardiovascular parameters to personal mobile phone. This work paved a simple, cost-effective and user-friendly approach for low power consumption measuring human pulse wave, which would be a competitive alternative to current complex cardiovascular monitoring systems and could be immediately and extensively adopted in a variety of applications, and ultimately improving our way of living.
101 2018 International Conference of Biomedical Information Perception & Microsystem TENG based pressure sensor for continuous measurement of human arterial pulse wave Keyu Meng1, Jin Yang*
1Department of Optoelectronic Engineering, Chongqing University, Chongqing 400044, P. R. China. *Email: [email protected]
Real-time heath monitoring and assessment is becoming more and more critical and indispensable, which largely contributes to the advancement of the field of wearable electronics for biomedical applications1,2. Pulse wave carries comprehensive information regarding human cardiovascular system, which is highly correlated to various physiological diseases related to heart. To measure the subtle changes in the pulse wave, various novel materials and nanotechnologies are applied to develop wearable sensors over the past decades, including piezoelectric materials, metal nanowires, and conductive fibers. However, the above mentioned wearable sensors are incapable of measuring the distinguishable arterial pulse wave owing to the insufficient sensitivity. Here, we reported a self-powered TENG based pressure sensor for continuous measurement of human arterial pulse wave in a noninvasive real-time manner. Additionally, a further step was taken to develop a cost-effective, wearable, user-friendly sensor system. Via a system-level optimization, all the system components can collaboratively work together for continuous and noninvasive human health assessment and monitoring.
Figure. (a) A schematic diagram of the signal management circuit. Photographs showing the low power consumption sensor system is worn against (b), human finger (c), wrist for real-time pulse wave measurement. And the real-time data can be received and display on mobile phone APP and oscilloscope simultaneously.
References 1. Fang, Y. et al. A highly shape-adaptive, stretchable design based on conductive liquid for energy harvesting and self-powered biomechanical monitoring. Sci. Adv. 2016, 2: e1501624. 2. Lai, Y., Deng, J., Zhang, S., Niu, S., Guo, H. & Wang, Z. Single‐Thread‐Based Wearable and Highly Stretchable Triboelectric Nanogenerators and Their Applications in Cloth‐Based Self‐Powered Human‐ Interactive and Biomedical Sensing.Adv. Funct. Mater. 2017, 27: 1604462.
102 2018 International Conference of Biomedical Information Perception & Microsystem D.07
Huaying Chen Associate Professor School of Mechanical Engineering and Automation Harbin Institute of Technology, Shenzhen
Biography
Huaying Chen received BE degree in Materials Science at Shandong University in 2004 and PhD degree in Biomedical Engineering at the University of New South Wales (Australia) in 2012. He is currently an associate professor in School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen. He is the member of Chinese Society of Micro and nano Technologies and director of Shenzhen Society of Micro and nano Technologies. His research interests include microfluidics, microscale fluid mechanics, point-of-care testing (POCT) and cell biology.
Distance-based quantification of blood glucose using a paper-based microfluidic device
This presentation introduces the design, fabrication, numerical study and characterization of a paper-based microfluidic device for quantitative measurement of glucose. The device consists of a loading zone for sample titration and a narrow detection zone with pre-deposited peroxidase and dye (3,3’-diaminobenzidine) for chemical reaction and colour development, respectively. The base of the device is Whatman No.1 filter paper and all fluid zones were confined by wax walls made by solid ink wax printer. Each device has a scale for quick reading. The glucose concentration was able to be quantitatively measured by the length of the colour in the detection zone. Numerical simulation was employed to study the chemical reaction rate and reaction flow on filter paper. Afterwards, samples with different glucose concentrations were detected using this device to demonstrate the stability, reproducibility and accuracy of this technology. The relationship of glucose concentration and colour distance was studied by image processing and regression analysis. As the detection of glucose is crucial for not only the treatment of diabetes but improving the life quality of diabetic people, this device is of great application potential since the detection is rapid, cost-effective and equipment-free.
103 2018 International Conference of Biomedical Information Perception & Microsystem Distance-based quantification of blood glucose using a paper-based microfluidic device Chang Chen1, Han Zhang2, Siwei Bai3 and Huaying Chen1* 1 School of Mechanical Engineering and Automation, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China 2 Department of Biological Engineering, Utah State University, 4105, Old Main Hill, Logan, UT, American 3 Department of Electrical and Computer Engineering, Technical University of Munich, Garching 85748, Germany *Email: [email protected]
In 2015, over 0.4 billion people were suffering from diabetes and around 5 million of them died from diabetes[1]. Detection of blood glucose is crucial for not only the treatment of diabetes but improving the life quality of diabetic people. As an emerging method, distance-based paper microfluidic chip has been developed for point-of-care testing due to its equipment free nature[2]. This study introduces the design, fabrication, numerical study and characterization of a paper-based microfluidic device for quantitative measurement of blood glucose. The device consists of a loading zone for sample titration and a narrow detection zone with pre-deposited peroxidase and dye (3,3’-diaminobenzidine) for chemical reaction and colour development, respectively (see 0). All fluid zones were confined by wax walls. The glucose concentration was able to be quantitatively measured by the length of the colour in the detection zone. Numerical simulation was employed to study the chemical reaction rate and reaction flow on filter paper. Afterwards, samples with different glucose concentrations were detected using this device to demonstrate the stability, reproducibility and accuracy of this this technology (see 0). This device is of great application potential since the detection is rapid, cost-effective and equipment-free.
Figure 1. Experimental results as the glucose concentration is 0.01 (left) to 1mg/ml (right).
References:
1. IDF Diabetes Atlas， 7TH edn. 2015, Brussels, Belgium: International Diabetes Federation. 2. Wei, X., et al., Microfluidic Distance Readout Sweet Hydrogel Integrated Paper-Based Analytical Device (muDiSH-PAD) for Visual Quantitative Point-of-Care Testing. Anal Chem, 2016. 88(4): p. 2345-52.
104 2018 International Conference of Biomedical Information Perception & Microsystem D.08
Zifei Xu Master student College of Mechanical & Electrical Engineering Nanjing University of Aeronautics & Astronautics
Photo Biography
Zifei Xu received the B.Eng. degree from the College of Mechanical & Electrical Engineering, Nanjing University of Aeronautics & Astronautics in 2016.He is currently a master student with the College of Mechanical & Electrical Engineering, Nanjing University of Aeronautics & Astronautics. His research filed is hardware design of electrical impedance tomography.
Development of a Portable Electrical Impedance Tomography System with Red Pitaya STEMlab
A portable Electrical Impedance Tomography (EIT) system for biological tissue measurements has been developed with Red Pitaya STEMlab. The Red Pitaya STEMlab is a portable device to realize voltage generation and data acquisition for the EIT system. The EIT system includes a modified howland circuit as a voltage controlled current source (VCCS), a high speed analogy multiplexer module, 8-electrode array and a personal computer. The generalized vector sampled pattern matching (GVSPM) algorithm is used to reconstruct the image generated by the EIT system. The reconstructed images by using Red Pitaya STEMlab are compared with commercial impedance analyzer IM3570 within frequencies of f = 100 KHz. The results show that the maximum difference of the image correlation between Red Pitaya STEMlab and IM3570 is 5.36%. These results verified that the developed portable EIT system with Red Pitaya STEMlab could measurement the biological tissue in a high accuracy at low cost.
105 2018 International Conference of Biomedical Information Perception & Microsystem Development of a Portable Electrical Impedance Tomography System with Red Pitaya STEMlab Zifei Xu1, Jiafeng Yao1* 1 College of Mechanical & Electrical Engineering, Nanjing University of Aeronautics & Astronautics, Nanjing, *Email: [email protected]
A portable Electrical Impedance Tomography (EIT) system for biological tissue measurements has been developed with Red Pitaya STEMlab. The Red Pitaya STEMlab is a portable device to realize voltage generation and data acquisition for the EIT system. The EIT system includes a modified howland circuit as a voltage controlled current source (VCCS), a high speed analogy multiplexer module, 8-electrode array and a personal computer. The generalized vector sampled pattern matching (GVSPM) algorithm is used to reconstruct the image generated by the EIT system. The reconstructed images by using Red Pitaya STEMlab are compared with commercial impedance analyzer IM3570 within frequencies of f = 100 KHz. The results show that the maximum difference of the image correlation between Red Pitaya STEMlab and IM3570 is 5.36%. These results verified that the developed portable EIT system with Red Pitaya STEMlab could measurement the biological tissue in a high accuracy at low cost.
Fig. 1. The overall structure of the EIT system
REFERENCES J. Yao, A. Sapkota, H. Konno, H. Obara, M. Sugawara, and M. Takei, “Noninvasive online measurement of particle size and concentration in liquid–particle mixture by estimating equivalent circuit of electrical double layer,” Particulate Science & Technology, 2015. Y. Yang, and J. Jia, “A multi-frequency electrical impedance tomography system for real-time 2D and 3D imaging,” Review of Scientific Instruments, vol. 88, no. 8, pp. 085110, 2017. X. Liu, J. Yao, Y. Cui, T. Zhao, H. Obara, and M. Takei, “Image Reconstruction under Contact Impedance Effect in Micro Electrical Impedance Tomography Sensors,” IEEE Transactions on Biomedical Circuits and Systems, 2018.
106 2018 International Conference of Biomedical Information Perception & Microsystem D.09
Lei Xi Associate Professor Department of Biomedical Engineering Southern University of Science and Technology
Biography
Lei Xi received his bachelor degree from Huazhong University of Science and Technology, Wuhan, China (2007) in optics, and finished his doctoral and post-doctoral training in the Department of Biomedical Engineering at the University of Florida, Gainesville, USA, in 2012 and 2014, respectively. Then, he worked at the University of Electronic Science and Technology of China (UESTC) from 2014 to 2018 as a professor. Now, he join the Department of Biomedical Engineering at the Southern University of Science and Technology (SUSTech). He is hosting the multifunctional optical imaging lab (MFOIL) in SUSTech and focusing on developing novel optical imaging techniques for different biomedical and clinical studies.
Miniaturization in photoacoustic microscopy and its applications in microfluidics
This work demonstrates a novel method of opto-acousto-fluidic microscopy for on-chip three-dimensional label-free detection of droplets and their encapsulated cells via their intrinsic absorbance of pulsed EM energy and subsequently trigged acoustic waves. The microscopic system shows a capability of full-field visualization of droplets with high spatial-temporal resolution, which can be used to study the dynamics of droplet formation. As the magnitude of opto-acousto signal is a direct reflection of absorption coefficient that depends on the concentration of analytes under study, this method can be used to map the distribution of analyte concentration within a droplet, which can further serve as a tool to analyse the droplet contents, such as cells with endogenous chromophores. As a proof-of-concept, red blood cells encapsulated in flowing droplets are studied with cytometric measurements through the detection of hemoglobins using this opto-acousto-fluidic microscopy technique. Given the obtained results, this proposed module shows its potential for further lab-on-chip applications.
107 2018 International Conference of Biomedical Information Perception & Microsystem Miniaturization in photoacoustic microscopy and its applications in microfluidics Lei Xi1*, Chaolong Song2 , Tian Jin1 1 Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, China. 2 School of Mechanical Engineering and Electronic Information, China University of Geosciences (Wuhan), Wuhan, China. *Email: [email protected]
Abstract text In this talk, we reports a novel method, opto-acousto-fluidic microscopy, for label free detection of droplets and cells in microfluidic networks. Leveraging the optoacoustic effect, the microscopic system possesses capabilities of visualizing flowing droplets, analyzing droplet contents, and detecting cell populations encapsulated in droplets via the sensing of acoustic waves induced by intrinsic light-absorbance of matters.
References Song C, et al. Opto-acousto-fluidic microscopy for three-dimensional label-free detection of droplets and cells in microchannels. Lab Chip, 10.1039/C8LC00106E, 2018. Jin T, et al. Portable Optical Resolution Photoacoustic Microscopy (pORPAM) for Volumetric Imaging of Multiscale Organisms. J Biophotonics 11:e201700250, 2018. Jin T, et al. Portable optical resolution photoacoustic microscopy (pORPAM) for human oral imaging. Opt. Lett. 42:4434-4437.
108 2018 International Conference of Biomedical Information Perception & Microsystem D.10
Wenxue Wang CEO Chengdu Tianxia120 Co., Ltd.
Biography
Wenxue Wang graduated from Chengdu University of Traditional Chinese Medicine in 1994. He received an MBA from Sichuan University in 2002 and an MPM from University of Electronic Science and Technology in 2017. He is also chief architect of Changhong's " Internet Plus" Health Care (Honorary), deputy secretary-general of the Committee of Experts on Intelligent Medical Care and Endowment Care of the Geriatrics Committee of the Chinese Gerontological Society, National Clinical Medical Research Center PI, and the National Ass ociation of Health Industry Enterprise Management Laboratory (Industry, study and research) Committee of Experts.The intelligent digital active health medical platform created by its leader, “Tianxia120”, is concentrating on high-quality medical resources, deeply merging medical IoT, medical and health big data, and medical artificial intelligence multi-dimensional technologies and applying iterative applications through its medical and Health application scenarios. , is committed to providing users with leading "professional- quality-active" intelligent digital active health products and services.
109 2018 International Conference of Biomedical Information Perception & Microsystem The application of Intelligent digital technology in the active health management Hong xian1*,Li Wang 2,Yongzhen Yang3, Wenxue Wang1 1*Geriatric Center,West China Hospital of Sichuan University,Chengdu,610041, China 2 Geriatrics Department , Leshan City People's Hospital ,Leshan,614000,China 3Respiratory Second Division ,Neijiang First People Hospital,Neijiang,641000,China 1 Chengdu Tianxia120 Co., Ltd., Chengdu,610041, China *Email: [email protected]
We are experiencing the medical and health prevention and treatment based on evidence-based me dicine theory and practice. We have stepped into the precise and active health management model based on the integration of "traditional medicine +" intelligent digital technologies such as Internet of things, big data, and artificial intelligence. 《The application of Intelligent digital technology in the active health management》 will use the tianxia120 digital medical system as an example to exchange and share active health management applicatio ns based on digital technology in the four main aspects of“ architecture design, platform function, innovation model and representative application case”. Experience and explore the available precision health care solutions.
References J. Guo, "Smartphone-Powered Electrochemical Biosensing Dongle for Emerging Medical IoTs Application," in IEEE Transactions on Industrial Informatics, vol. PP, no. 99, pp. 1-1. doi: 10.1109/TII.2017.2777145 J. Guo, Smartphone-Powered Electrochemical Dongle for Point-of-Care Monitoring of Blood β-Ketone, Analytical Chemistry, vol. 89, no. 17, pp. 8609-8613, 2017.
110 2018 International Conference of Biomedical Information Perception & Microsystem D.11
Hongpeng Zhang Professor Marine Engineering College Dalian Maritime University
Biography
Zhang Hongpeng received his Ph.D. degree in Marine engineering from the Dalian Maritime University in 2005. He is currently a professor of Marine Engineering College, Dalian Maritime University. He has published more than 50 journal papers. His research interests cover mainly microfluidic application in marine engineering.
111 2018 International Conference of Biomedical Information Perception & Microsystem Design of a high-flux microfluidic fluid oil detection chip
Haotian Shi, Hongpeng Zhang*, Lin Zeng, Guangtao Sun Marine Engineering College, Dalian Maritime University, Dalian 116026, China *Email: [email protected]
Abstract text This paper presents a new design of inductive-capacitive microfluidic detection chip. This new chip improves the flux of microfluidic chip by a flow passage which is closed to the inner ring of the coil. The cross-sectional area of the flow channel is increased by 8 times. Considering that the metal particles in the oil are greatly affected by the gravity in the annular flow passage, the particles are easy to deposit and block the detection flow channel. Therefore, the vertical flow passage is adopted to avoid this phenomenon. Through comparison experiments with the original detection chip, it is verified that the detection chip designed in this paper can improve the throughput without reducing the detection accuracy. The sensor's inductance parameter detection mode and capacitance parameter detection mode can work without interfering each other, so as to distinguish and detect four kinds of pollutants of ferromagnetic metal particles, non-ferromagnetic metal particles, water droplets and air bubbles in the hydraulic oil. This design provides a new method for rapid detection of high-flux microfluidic fluids.
2-3 references are preferred. Zhang H, Zeng L, Teng H, et al. A Novel On-Chip Impedance Sensor for the Detection of Particle Contamination in Hydraulic Oil[J]. Micromachines, 2017, 8(8):249. Fan H, Zhang Y, Yuan C. Effect of the Radial Distribution of the Wear Debris Position on the Testing Results of Inductive Wear Debris Sensor[J]. Chinese Journal of Sensors & Actuators, 2010, 23(7):958-962.
112 2018 International Conference of Biomedical Information Perception & Microsystem D.12
Shuting Pan Ph.D. candidate College of Precision Instrument and Optoelectronics Engineering Tianjin University, China
Biography
Shuting Pan received the B.S. degrees in Measuring and Control Technology and Instrumentations from Tianjin University in 2014. She is currently pursuing the Ph.D degree in Instrument Science and Technology, Tianjin University. She has published 2 journal papers. Her research interests cover mainly BioMEMS and the application of acoustic device in protein detection.
113 2018 International Conference of Biomedical Information Perception & Microsystem A label-free immunosensor based on gigahertz resonator enhanced fibre optic SPR Shuting Pan1, Xian Chen1, Wei Pang2, Xuexin Duan1* 1 State Key Laboratory of Precision Measuring Technology & Instruments, Tianjin University, Tianjin, China 2 College of Precision Instrument and Optoelectronics Engineering, Tianjin University, China *Email: [email protected]
Surface-based biosensors are fundamentally limited by the diffusion of the target biomolecules to the sensor surface [1]. Here, we reported a novel approach to break the mass transfer limitation and enhance the biomolecular surface binding by integrating gigahertz acoustic device into an optoelectronic bioassay. Figure 1(a) shows the schematic of the acoustically enhanced biosensing system. The resonator is fabricated by standard MEMS process and fixed below the optic fibre. Target proteins can be dedicatedly manipulated by tuning the input power. The real-time binding information can be quantitatively obtained by SPR.
(a) Probe antibody Directional Target antigen Coupler Light source Chamber SPR probe
Resonator Spectrometer
(b) No power (c) 0 mm no power (d) Power to non-specific binding 15 0 mm 4 10 mW Power to specific binding 50 mW 0.1 mm 0 100 mW 0.2 mm 500mW 200 mW 10 0.5 mm -5 2 5 -10
Wavelength Wavelength Shift (nm) 0 Wavelength Shift (nm) 0 Wavelength Shift (nm) Power off -15 aa 0min0min 5min 10min 15min15min bb 0 0min0min5 10 5min5min15 10min10min20 15min15min0 5 10 15 20 0 2 4 4.00mW4.00mW 4mW4mW Time (min) Time (min) Time (min) a 0min 5minQ=41Q=41 10min 15min b 0min 500pM5min500pM 10min 15min Specific bindings NSBs 0 mm b b No power 50mWb 0.5 mm 0.2 mm 0 mm 0.1 mm 4.00mW a a 0min0min a5min5min aa10min0min10min 0min0min15min4mW5min15min 5min5min10min 10min0min15min0min 15min5min5min (Nobb10min0min power)10min0min0min15min5min15min5min5min10min 10min10min15min15min15min (e) (f) 4.00mW4.00mW 1.00mW1.00mW 4.00mW 4.00mW4.00mW 500pM 4mW4mW 4mW4mW 4mW 4mW4mW Q=41 Q=41Q=41 Q=41Q=41 Q=41 Q=41Q=41 500pM500pM 50pM50pM 500pM 500pM500pM 20 μm 20 μm 100 μm 100 μm
1.00mW1.00mW 0.25mW0.25mW 1.00mW 1.00mW1.00mW 4mW4mW 4mW4mW 4mW 4mW4mW 1.00mW Q=41 Q=41Q=41 Q=41Q=41 4mW 5pM5pM 50pM50pM Q=41 Q=41 50pM50pMFigure 1. (a) Schematic50pM of the gigahertz ultrasonic enhanced fibre optic SPR. Enhanced Q=41 50pM SPR and florescent results with different input power (b) and relative distance (c). NSB 0.25mW0.25mW 0.00mW0.00mW 0.25mW 0.25mW0.25mW 4mW4mW 00mWmW 4mW 4mW4mW Q=41 Q=41Q=41 Q=41 Q=41 Q=41Q=41 5pMremoval5pM 5nM5nM (d) and5pM the5pM 5pMcomplementary fluorescent results before (e) and after (f) removal 0.25mW 4mW Figure 1(b)-(f) show the real-time enhanced SPR results and verified by fluorescent 4.00mW4.00mW 0.00mW0.00mW 0mW Q=41 0.00mW0.00mW 0.00mW 0mW0mW 0mW 0mW Q=33Q=33 Q=41Q=41 5pM measurementscc (Cy3-labeled5nM5nM IgG). When applying low power (<200mw), acoustic streaming will Q=41Q=41 Q=41 5nM5nM 5nM accelerate the targets binding to the sensor surface. We compared different input power (figure 4.00mW4.00mW 4.00mW4.00mW 4.00mW 1(b)) and the distance between the resonator to the sensor (figure 1(c)). It turns out that the 0.00mW Q=33Q=33 cc Q=33Q=33 Q=33 0mW bindingc cenhancement4mW4mW isc 4mWoptimized4mW 4mW4mW by applying0mW0mW 50 mW with 0.1 mm distance. It is interesting to Q=41 5pM5pM 50pM50pM 500pM500pM 5nM5nM 5nM note that when applying high power (500 mW), drag forces are generated which can be used to
remove the nonspecific bindings4mW4mW (NSBs)4mW4mW (figure4mW4mW 1(d)). 0mW0mW 4mW 4mW 4mW 0mW 4mW 4mW 4mW4mW 5pM5pM4mW0mW 50pM50pM4mW 500pM500pM0mW 5nM5nM 4.00mW Reference5pM 5pM 50pM50pM 500pM5pM500pM 50pM5nM5nM 500pM 5nM Q=33 c [1] Duan X, et al. Complementary metal oxide semiconductor-compatible silicon nanowire biofield-effect transistors as affinity biosensors. Nanomedicine, 2013, 8(11): 1839-1851.
4mW 4mW 4mW 0mW 5pM 50pM 500pM 5nM
114 2018 International Conference of Biomedical Information Perception & Microsystem D.13
Li Changhao Student School of Life Science and Technology University of Electronic Science and Technology of China
Biography
Li, Changhao is currently an undergraduate student majoring in biomedical enginering. He is supposed to obtain his Bachelor's degree in June, 2019 from University of Electronic Science and Technology of China. His research interest covers mainly ultrasound imaging, medical artificial intelligence and biomedial systems.
115 2018 International Conference of Biomedical Information Perception & Microsystem Measurement and Analysis of Arm Muscle Movement with Ultrasound Imaging Changhao Li1, Quanyong Wang1, Zhe Wu1* 1 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China *Email: [email protected]
Abstract: Ultrasound has been widely used in detection of muscle morphology and judgement of the movement pattern of muscles. As an effective way to understand dynamic motion patterns, ultrasound imaging can detect the movement of the deep muscles in human body. To investigate the muscle control mechanism for elbow flexion and extension, we study the movement of upper arm muscles with ultrasound scanning. An observation shows that angles between biceps brachii, brachialis and triceps brachii change correspondingly if elbow flexes or extends. Therefore, we assume that the upper arm muscle movement patterns in ultrasound images and changes of angle between upper and lower arms are related. To verify this assumption, we collect ultrasound images of different muscles of the upper arm and measure the corresponding angle between upper and lower arms. Then we extract image features to detect the angle between muscles and the muscles’ grayscale using image processing techniques, which analyses the movements of muscles qualitatively and quantitatively, thus finding the relationship between these parameters and the corresponding arm angles. It is concluded that the angles between upper arm muscles and muscles’ grayscale do change with the elbow flexion/extension angles and external forces. Future work will focus on evaluating the muscle movement patterns with AI (Artificial Intelligence) and consequently we can predict the movement of arm muscles.
Keywords: ultrasound imaging, muscle movement, elbow flexion and extension, muscle angles, muscles’ grayscale Reference: 1. Eranki, Avinash, et al. "A Novel Application of Musculoskeletal Ultrasound Imaging. Journal of Visualized Experiments,2013 Sep 17;(79):e50595. 2. Castellini, C, G. Passig, and E. Zarka. "Using ultrasound images of the forearm to predict finger positions." IEEE Transactions on Neural Systems & Rehabilitation Engineering,2012 Jul 27:788-797. 3. Castellini, C, et al. "Proceedings of the first workshop on Peripheral Machine Interfaces: going beyond traditional surface electromyography." Frontiers in Neurorobotics, 2014 Aug 15:22.
116 2018 International Conference of Biomedical Information Perception & Microsystem
E. Poster
117 2018 International Conference of Biomedical Information Perception & Microsystem E.01 GPU-based Ultrasound Plane-wave Spatial Compound Imaging Zhenjie Tian1, Bo Yang1, Wenping Wang2, Quanyong Wang1, Zhe Wu1*, 1 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China 2Ultimedical Inc., Chengdu, China *Email: [email protected]
Abstract: In the field of diagnostic ultrasound imaging, spatial compounding is a frequently used method which reduces the speckle noise by averaging images from different incident angles. This technique increases the image quality but reduces the frame rate. Plane-wave imaging is to obtain every image by only one transmission and reception. Compared to the method of traditional ultrasound imaging, plane-wave imaging acquires images faster, thus increasing frame rates. However, it needs more computational capacity because it does beamforming in the backend processor. In this paper, we propose plane-wave multi-angle spatial compound imaging, which is based on CUDA (Compute Unified Device Architecture) parallel processing platform. The method we proposed enhances both spatial resolution and temporal resolution. A comparison with the traditional ultrasound imaging shows that the spatial resolution is improved, and the temporal resolution is 10 to 80 times faster with the number of steering of 17.
Keywords: spatial compound imaging, GPU, plane-wave, beamforming, multi-angle Reference: 1. Bruneel C, Torguet R, Rouvaen KM, et al. Ultrafast echotomographic system using optical processing of ultrasonic signals [J]. Applied Physics Letters, 1977, 30(8): 371-373. 2. A. Achim, A. Bezerianos, and P. Tsakalides, Novel Bayesian multiscale method for speckle removal in medical ultrasound images, IEEE Trans. Medical Imaging, vol. 20, pp. 772-783, August 2001. 3. S. K. Jespersen, J. E. Wilhjelm, and H. Sillesen, “Multi-angle compound imaging,” Ultrasonic imaging, vol. 20, no. 2, pp. 81–102, 1998.
118 2018 International Conference of Biomedical Information Perception & Microsystem E.02
A pleiotropic micro nano-carrier based on Ginsenoside Rg3 encapsulated Fe3O4@SiO2 microbubbles for both drug delivery and molecular imaging Tingting Zheng1,#, Yu Shi1,#, Xing Ma2,#, Jiao Peng3, Ziqian Zhou1,Yuseng Zhang1, Haitao Xiao4, Yuhuan Liu3, Ting Jin1, Li Liu1,* Huanhuan Feng2,*, and Yun Chen1,* 1 Shenzhen Key Laboratory for Drug Addiction and Medication Safety, Department of Ultrasound, Peking University Shenzhen Hospital & Biomedical Research Institute, Shenzhen-PKU-HKUST Medical Center, 518036 Shenzhen, China. 2 Harbin Institute of Technology (Shenzhen), 518055 Shenzhen, China. 3 Department of Pharmacy, Peking University Shenzhen Hospital, 518036 Shenzhen, China. 4 School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, 518060 Shenzhen, China. *Email: [email protected], [email protected], [email protected]
Silica coated magnetite (Fe3O4@SiO2) core-shell nanoparticles (NPs) has great potential in preclinical studies to be applied as magnetic resonance imaging (MRI) contrast agents. However, according to strong magnetism property of Fe3O4 core, higher concentration of Fe3O4@SiO2 always ends up with serious aggregations. Poor aqueous stabilities of these nanoparticles limit their applications. Here, a pleiotropic micro nano-carrier is reported for both drug delivery and magnetic resonance imaging. In brief, Fe3O4@SiO2 nanoparticles with high colloidal stability have been synthesized. Average size of MNPs are around 220 nm, with 20 nm Fe3O4 NPs as seeds in core. We achieve the system’s colloidal stabilities by encapsulating these magnetic NPs (MNPs) into Ginsenoside Rg3-microbubbles, yielding M-RMbs. Here Rg3 is a therapeutic compound that has been observed in cell models as having an inhibitory effect on the cell growth of various cancer cells. In addition to its therapeutic effect, it is adopted in our system also as tumor targeting motif in model of colon cancer, and as water soluble block improving surface stability for MNPs. All these core-shell MNPs are characterized by X-ray diffraction (XRD) and UV-Vis adsorption spectra. Finally, targeted molecular imaging of these M-RMbs is tested via bioluminescence imaging, ultrasound imaging and MRI imaging systems. All results points out that the monodispersed M-RMbs are promising micro- nano-carriers which can be applied as both a pleiotropic contrast and a drug carrier for colon cancer.
Figure 1. (a)shows SEM image of Fe3O4@SiO2. (b) shows MRI images which are monitored at different time after tail intravenous injection of M-RMbs on SD rats with subcutaneous tumor. Reference: [1] Jiapu Jiao; Dandan Xu; Yuhuan Liu; Weiwei Zhao, Jiaheng Zhang, Tingting Zheng; Huanhuan Feng and Xing Ma, Mini-Emulsion Fabricated Magnetic and Fluorescent Hybrid Janus Micro-motors. Micromachines, 2018, 9, (83), 1-10. [2] Zheng, T.; Perona Martinez, F. P.; Storm, I. M.; Rombouts, W.; Sprakel, J.; Schirhagl, R.; de Vries, R., Recombinant protein polymers for colloidal stabilization and improvement of cellular uptake of diamond nanosensors. Analytical Chemistry, 2017, 89, (23), 12812-12820.
119 2018 International Conference of Biomedical Information Perception & Microsystem E.03 Reflection of the Traceable Mode of Establishing High-quality for the TCM Based on the Traceability Technology of Safety and Interconnection Shu-ting ZHAO*, Ming-yi SHI, Ye Zhang, Shi-chao ZHENG (Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, China) *Email: [email protected]
[Abstract] By analyzing the construction level and the key technology for the traceability system of Traditional Chinese Medicine in our country, it is important to research the reason of information island. Combined with the modern information technology development process, the research put forward to build the traceability system, covering planting link, production processing link, commercial circulation and hospitals use link tracing system of traditional Chinese medicines which is based on the block chain technology and Internet of things technology. Finally, it could ensure the data security, break trace information island, reduce the asymmetry of information to the greatest degree, and put an end to fraud, illegal phenomenon existing in the TCM industry. Thus, the high quality premium TCM will raise the enterprise brand, enhance the integrity of the whole TCM industry, and reinforce the foundation for the modernization and internationalization development of TCM.
References [1] China Food and Drug Administration. Opinions of CFDA on Promoting the Improvement of the Traceability System by the Food and Drug Production and Operation Enterprises (Office of FDA No. 122 (2016) [EB/OL]. http://www.sda.gov.cn/WS01/CL0852/164862.html [2] C. Ma, et al. Ethnical Moral Education Triggered from Medication Misadventure [J]. Chinese Folk Medicine, 2010, (7):62+64. [3] M. Shi, et al. Research Status of Traceability System of TCM Quality [J]. Journal of Chengdu University of TCM, 2016, 39 (3):109-113. [4] Implementation of Medicinal Materials Tracing Engineering for Ensuring the Quality Safety of TCM – Introduction to Traceability System of TCM Quality, Modern Chinese Medicine, 2015, 17 (8):748. [5] Y. Zhang. Product Traceability System [M]. Beijing: Tsinghua University Press, 2013, 70. [6] M. Shi, et al. Analysis on Research Status of Traceability System of TCM Quality [J]. Journal of Chengdu University of TCM, 2016, 39 (4):103-106. [7] Z. Gao, et al. Analysis on the Cause and Prevention of Medication Misadventure [J]. Drug Evaluation, 2012, 9 (17):42-44. [8] Y. Zhang. Product Traceability System [M]. Beijing: Tsinghua University Press, 2013, 10-15. [9] Satoshi Nakamoto S. Bitcoin: a peer-to-peer electronic cash system [J]. Consulted, 2009.
120 2018 International Conference of Biomedical Information Perception & Microsystem E.04 Algorithm Research on Correlation Model between TCM Constitution and Physical Examination Index Based on RBF neural network Yue Luo1, Yu-nan Liu1, Rui-xian Tang1, Bing Lin2, Yuan Gao1, Chuan-biao Wen1* 1Chengdu University of Traditional Chinese Medicine 2Affiliated Hospital of Chengdu University of Traditional Chinese Medicine *Email:[email protected]
Abstract: Purpose: To construct the correlation model between Traditional Chinese Medicine (TCM) constitution and physical examination indexes by using RBF neural network. Methods: First, clean and classify the original data from physical examination index and TCM constitution types of 600 physical examinees raw data to form the valid data. Next, the valid data is divided into study group and test group. Then, construct the correlation model between TCM constitution and physical examination index by using RBF neural network and study group data. Finally, use test group data to verify the correlation model. Results: In the selected samples, the accuracy of blood routine indexes-TCM constitution correlation model was 80%; the accuracy of renal function indexes-TCM constitution correlation model was 100% ; the accuracy of blood routine indexes(male and female)-TCM constitution correlation model were respectively 100% and 88.8%; the accuracy of urine routine indexes- TCM constitution correlation model was 84% correct; the accuracy of full set of blood transfusion indexes-TCM constitution correlation model was 100%.Conclusions: It is proved that there is strong correlation between TCM constitution and physical examination indexes for the sample selected in this paper. It is practicable to identify TCM constitution based-on the correlation model between TCM constitution and physical examination indexes. Keywords: TCM Constitution; Physical Examination Indexes; Correlation Model; RBF neural network
References:
[1]Q.Wang.Introduction and the Factors Forming Diffrent Physical Constitutions: Theory of TCM Constitution,1st ed. Jiangsu,China:Jiangsu scien. and tech. ,1982,pp.1-2+26-35. [2]H. J.Yu,C.Z.Chen,S.Zhang and J.N.Zhou.Intelligent Diagnosis Based on Neural Network.1st ed .,Beijing,China:Metallurgical Industry,2000,pp.109. [3]J.Yu,Q.L.Pan,J.F.Yang and C.X.Zhu et al.Correlations of Complete Blood Count with Alanine a nd Aspartate Transaminase in Chinese Subjects and Prediction Based on BackPropagation Artifici al Neural Network (BP-ANN): Medi. Scien.,vol.23,pp.3001-3009,Jun.2017,DOI:10.12659 / MSM .901202.
121 2018 International Conference of Biomedical Information Perception & Microsystem E.05 Research on Intelligent Syndrome Differentiation Model of Stomachache in Traditional Chinese Medicine Based on BP Neural Network Liang Zhao, Ye Zhang, Yue Cao , Hua Ye* School of Medical Information Engineering， Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 611137, China
Abstract—A TCM(Traditional Chinese Medicine) intelligent syndrome differentiation model of stomachache based on BP neural network is put forward in this paper. The clinical electronic medical record data of ‘digital diagnosis and treatment platform of TCM’ is used as a data set, and Matlab is used as the model simulation platform, and the Quasi-Newton method is used to build the double hidden layer BP neural network model of TCM intelligent syndrome differentiation for stomachache. The testing results shows that the syndrome accuracy and diagnosis accuracy of ‘liver-stomach disharmony’ and ‘stomach yang deficiency’ are very high, above 95%, by using network predictive model. It shows that the intelligent syndrome differentiation model of TCM can fully approach the real side of syndrome differentiation by effectively using the autonomous learning ability of BP neural network, and shows excellent predicted ability of syndrome differentiation. What’s more, new reliable clinical data of TCM are uploaded every day in the ‘digital diagnosis and treatment platform of TCM’. If these data are used to improve the intelligent syndrome differentiation model, it is expected to promote the large-scale application of TCM intelligent syndrome differentiation in clinical auxiliary diagnosis of TCM. Key words—Traditional Chinese Medicine, intelligent syndrome differentiation, stomachache, BP neural network, Quasi-Newton method
122 2018 International Conference of Biomedical Information Perception & Microsystem E.06 A micro-rod magnetic SERS probe fabrication & application in biomedical detection Yuhuan Liu, Jiapu Jiao, Xing Ma*, Huanhuan Feng* School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), 518055, Shenzhen, China *Email: [email protected]
We have fabricate a magnetic SERS probe for biomedical detection through a handy modified Stöber reaction. The silica reaction is occurred under magnetic field to align the magnetic particles and string them together to form micro rob structure. Its geographical magnetic moment offers a perfect platform for precise movement and locomotion manipulation under magnetic field. We can make it go through a micro scaled Maze easily to reach targeted area via magnetic guiding and driving. Not only it could move as we designed but also it could rotate with demanding angular velocity for some very special application such as cell tissue depletion experiments. Its surface satellite doping silver is just adding SERS (Surface Enhanced Raman Spectrum) as an extra function for its biomedical detection. The detection ability is checked by Crystal Violet in vitro. The full potential of biomedical sensor in vivo will be explored in view. Its multi-functional ability make it an outstanding candidate for further biomedical application such as micro surgery robot, biomedical micro sensor and of course targeted drug delivery mediation.
Figure 1 (a) is the SEM of SERS probe with silver satellite surface doped. Scale bar is 500 nm. (b) is the Raman spectrum of micro rob magnetic SERS probe with Crystal Violet in vitro.
Reference: [1] J. Du, C. Jing, Preparation of Thiol Modified Fe3O4@Ag Magnetic SERS Probe for PAHs Detection and Identification, The Journal of Physical Chemistry C, 115 (2011) 17829-17835. [2] D. Song, R. Yang, C. Wang, R. Xiao, F. Long, Reusable nanosilver-coated magnetic particles for ultrasensitive SERS-based detection of malachite green in water samples, Scientific Reports, 6 (2016) 22870.
123 2018 International Conference of Biomedical Information Perception & Microsystem E.07 Application and Development of Artificial Intelligence in TCM Electrical Acupoint Stimulation Equipment Yuan Gao1 1Chengdu University of TCM *Email:[email protected]
Abstract: The combination of artificial intelligence theory and TCM treatment equipment has a good application and development prospect in standardization of parameter, control of treatment and data processing. The electrical acupoint stimulation equipment is the one of the traditional chinese medicine therapeutic tools, which is most widely used in clinical medicine. This paper reviewed the research on the parameter setting and treatment effects of traditional electrical acupoint stimulation equipment, and discussed the application of artificial intelligence theory in the standardization of parameters. In addition, the nontraditional electrical acupoint stimulation equipment can be divided into many types, such as multifunctional type, portable type, special disease type, and the intelligent application of different kinds of equipment in the control of treatment and data processing is an important direction of development.
124 2018 International Conference of Biomedical Information Perception & Microsystem E.08 Feasible Recipes for Cytop Patterning Yalei Qiu1 , Shu Yang1, Kuang Sheng1* 1College of Electrical Engineering, Zhejiang University, Hangzhou, China *Email: [email protected]
Abstract Cytop is a commercially-available amorphous fluoropolymer with excellent characteristics including electric insulation, water and oil repellency, chemical resistance, and moisture-proof property, making it an attractive material as hydrophobic layer in EWOD devices. However, its highly hydrophobic surface makes it difficult for photoresist to be directly coated on the surface. To pattern Cytop, plasma treatment prior to applying photoresist was required to promote the adhesion between photoresist and Cytop coating. This approach inevitably causes hydrophobicity loss in the final EWOD devices. Thus, a damage-reduced recipe for Cytop patterning is urgently needed. In this paper, we first characterized the damage caused by two categories of surface treatment methods: plasma treatment and metal treatment. Parameters such as plasma gas source (Ar/O2), plasma treatment time (0s/60s/120s/180s/240s/300s/400s/600s), metal target (Al/Cu/Cr/Au), metal deposition process (Magnetron Sputtering/E-beam Evaporation) were varied. Film thickness, wettability, and roughness were quantified by Ellipsometry measurements, Contact Angle measurements, and Atom Force Microscope (AFM), respectively. We then evaluated the effectiveness of annealing in damage reduction. Experimental results show that: 1) annealing is necessary in restoring hydrophobicity as well as smoothing surface; 2) specified film thickness can be obtained by controlling plasma treatment time; 3) Ar/O2 plasma+AZ5214 soft mask+annealing is a feasible recipe; 4) Al/Cu/Cr/Au hard mask+annealing is feasible as well.
References Agnarsson B, Halldorsson J, Arnfinnsdottir N, et al. Fabrication of planar polymer waveguides for evanescent-wave sensing in aqueous environments[J]. Microelectronic Engineering, 2010, 87(1): 56-61. Kobayashi T, Shimizu K, Kaizuma Y, et al. Formation of superhydrophobic/superhydrophilic patterns by combination of nanostructure-imprinted perfluoropolymer and nanostructured silicon oxide for biological droplet generation[J]. Applied Physics Letters, 2011, 98(12): 123706.
125 2018 International Conference of Biomedical Information Perception & Microsystem E.09 Deformation response measurement of cells manipulated by optical tweezers based on laser interference Liu Jiaqi1 (presenting author underscored), Zhang Fan2, Zhu Lianqing2* 1 State Key Laboratory of Precision Measuring Technology and Instruments, School of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China 2 Beijing Laboratory for Biomedical Detection Technology and Instrument, Beijing Information Science & Technology University, Beijing 100192, China *[email protected]
A method to measure deformation response based on laser interference is proposed to characterize the cell deformation in situ while it is manipulated by optical tweezers. It is implemented experimentally by integrating a laser illuminating system and optical tweezers with an inverted microscope. Fringes formed by the interference between the transmitted and reflected lights are recorded by a CMOS camera. From which, cell height information can be derived and 3D morphology of the cell constructed. To validate this method, 3D morphologies of HeLa cells in its static state and in detachment process are measured. Subsequently 3D dynamic deformation response of a human red blood cell is measured while it is manipulated by optical tweezers. This method precludes the requirement of complex optical alignment, allows readily integration with optical tweezers, and enables dynamically measurement of deformation response on a conventional inverted microscope.
Schematic of laser interference measuring system References A. Ashkin, J. M. Dziedzic, J. E. Bjorkholm, and S. Chu, “Observation of a single-beam gradient force optical trap for dielectric particles,” Opt. Lett. 11(5), 288–290 (1986). S. Hénon, G. Lenormand, A. Richert, and F. Gallet, “A new determination of the shear modulus of the human erythrocyte membrane using optical tweezers,” Biophys. J. 76(2), 1145–1151 (1999). Gabriel Popescu, Takahiro Ikeda, “Optical Measurement of Cell Membrane Tension,” Physical review letters 97 (21), 218101 (2006).
126 2018 International Conference of Biomedical Information Perception & Microsystem E.10 Microparticle Separation via Traveling Surface Acoustic Waves (TSAWs) Zhichao Ma1, David J. Collins1, Ye Ai1* 1Singapore University of Technology and Design *Email: [email protected]
Separation of microscopic particles and cells in microfluidic platforms plays an important role in biological analyses and medical diagnostics, miniaturising bulky and expensive biomedical testing equipment into portable and affordable microfluidic devices. Acoustophoresis, particle movement in acoustic field, with good biocompatibility and low power consumption, has emerged as a promising, non-invasive method for microparticle manipulation. Based on the nonlinear correlation between the acoustic radiation force with particle size, density and sound speed in traveling acoustic field, microparticle separation based on mechanical properties and single-actuator bandpass microparticle filtration are demonstrated here.
References 1. Hasegawa T. and Yosioka K. Acoustic-Radiation Force On A Solid Elastic Sphere. The Journal of the Acoustical Society of America (1969). 2. Ma Z., Collins D. J. and Ai Y. A Detachable Acoustofluidic System For Particle Separation Via A Travelling Surface Acoustic Wave. Analytical Chemistry (2016). 3. Ma Z., Collins D. J., Guo J. and Ai Y. Mechanical Properties Based Particle Separation Via Traveling Surface Acoustic Wave. Analytical Chemistry (2016).
127 2018 International Conference of Biomedical Information Perception & Microsystem E.11 Enhanced biofilm distribution and power generation of membraneless microbial fuel cells with a serpentine microchannel Xian Luo1,2,3, Yan Qiao1,2,3* 1Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, P. R. China 400715 2 Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China 3 Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Southwest University, Chongqing 400715, China *Email: [email protected]
Abstract: Microchannel microbial fuel cells (MFCs) with co-laminar microfluidic structure are superior to other micro-sized MFCs according to their low internal resistance and relative high power density. However, the biofilm loading amount in the typical Y shaped device is relative low, which apparently limits the current generation performance. In this study, we developed a membraneless microfluidic microbial fuel cell(MMFC) with serpentine microchannel to enhance the biofilm adhesion and promote the power generation of the device. Owing to the merit of laminar flow, the proposed MMFC was working well without proton exchange membrane. At the same time, the serpentine microchannel greatly increased the biofilm loading amount. The results show that this MMFC achieves a peak power density of 360 mW/m2 with the optimal channel shape and the flow rate of 5 ml/h. Meanwhile, this device possesses much shorter start-up time and much longer duration time at high current plateau than the previous reported MMFCs. The presented MMFC appears promising for bio-chip technology and extends the scope of microfluidic energy.
References: D.Ye, Y. Yang, J. Li, X. Zhu, Q. Liao, B. Deng and R. Chen, International Journal of Hydrogen Energy, 2013, 38, 15710-15715. Y. Yang, D. Ye, J. Li, X. Zhu, Q. Liao and B. Zhang, Journal of Power Sources, 2016, 324, 113-125.
128 2018 International Conference of Biomedical Information Perception & Microsystem E.12 Integrated treatment and Detection of microalgae cells on a microfluidic chip Junsheng Wang 1, *, Ge Wang 1, Mengmeng Chen1, Yanjuan Wang1, Xinxiang Pan2, Dongqing Li3 1. College of Information and Science Technology, Dalian Maritime University, Dalian, 116026, China; 2. College of Marine Engineering, Dalian Maritime University, Dalian, 116026, China; 3. Department of Mechanical & Mechatronics Engineering, University of Waterloo, Waterloo, ON, Canada, N2L3G1 * Correspondence: [email protected]; Tel.: +86-411-84723190 Abstract:Marine transportation occupies an important position in Chinese economic development, the ship is an important means of transport for marine transportation. The large amount of microalgae cells and other exotic organisms carried in ballast water can seriously damage the marine ecological environment. The search for a method for efficient treatment of microalgae cells and the rapid detection of the treatment effect have become an important part of the ballast water treatment. This article used chemical treatment to treat microalgae cells in ballast water, which was faster and more efficient than other methods. A concentration gradient generation chip based on a microfluidic platform was proposed, that used the principle of circuit equivalency and can quickly generate any desired concentration gradient. It was applied to the microalgae treatment system for the first time. The microalgae were treated Simultaneously with various concentration solutions generated by the concentration gradient generator and the treatment cycle was shortened. In this paper, the principle of laser induced chlorophyll fluorescence was used to monitor the whole activity of microalgae in real time by using the automatic microalgae detection system, which avoided the disadvantages of tedious operation, long detection cycle, and complex analysis process. The first combination of the concentration gradient chip and the automatic high-throughput microalgae detection system can quickly screen out the optimal concentration and treatment time of the reagent needed for the treatment of microalgae. These results are of great significance for the future treatment of ship ballast water and detection of treatment effect, and provide new ideas and references. Keywords:microfluidic;concentration gradient generator; microalgae treatment; fluorescence detection
Figure. Schematic of treatment and detection system 1. Yang C G, Wu Y F, Xu Z R, et al. A radial microfluidic concentration gradient generator with high-density channels for cell apoptosis assay[J]. Lab on A Chip, 2011, 11(19):3305. 2. Tsolaki E, Diamadopoulos E. Technologies for ballast water treatment: a review[J]. Journal of Chemical Technology & Biotechnology Biotechnology, 2010, 85(1):19-32. 3. Choi J, Kang M, Jung J H. Integrated micro-optofluidic platform for real-time detection of airborne microorganisms[J]. Scientific Reports, 2015, 5:15983.3.996.
129 2018 International Conference of Biomedical Information Perception & Microsystem
130 2018 International Conference of Biomedical Information Perception & Microsystem E.13 Photoacoustofluidics: combining light and sound in microfluidic chip Yongjian Zhao1, Tingyang Duan1, Yuanjin Zheng2, and Fei Gao1,* 1 Hybrid Imaging System Laboratory, School of Information Science and Technology, ShanghaiTech University, Shanghai, China 2 School of Electrical and Electronic Engineering, Nanyang Technological University, Shanghai, China *Email: [email protected], corresponding author
Abstract：New imaging technology based on photoacoustic (PA) effect is widely used in sensing and imaging in biomedical applications in recent years, from the subcutaneous blood vessel microscopic imaging to the small volume overall imaging. Due to the high optical contrast and high acoustic resolution, and its breaking the optical diffusion limit, functional PA imaging has been proposed to detect the wavelength dependence of light absorption. Although PA sensing has been studied with remarkable results. it is still rarely explored in compact microfluidic environment. Here, we review a SAW-PA integrated device integrating a PA sensor and a Rayleigh SAW device, which utilizes a piezoelectric substrate as the acoustic propagation and detection mechanism, and uses this device to explore the photoacoustic properties of standard dyes and Au nanoparticles in microchannels (Fig. 1 and 2). Experiments show that 80% of the high-mode conversion efficiency can be achieved with optimized design of SAW-PA device. The SAW-PA platform can also be applied to biological/chemical sensing by detecting changes in light absorption spectra induced by receptor-analyte binding. Key words: Photoacoustic microfluidic chip, optical absorption;
Fig 1 SAW-PA sensor with the bonded PDMS microfluidic channel
Fig 2 (a) UV/Vis absorption spectrum of erythrosine and brilliant blue (b) Measured SAW-PA signal intensity from the erythrosine and brilliant blue dyes at different laser wavelength
Reference
[1]Kishor, Rahul, Fei Gao, et al. Photoacoustic Induced Surface Acoustic Wave Sensor for Concurrent Opto-Mechanical Microfluidic Sensing of Dyes and Plasmonic Nanoparticles. RSC Advances.2016,09: 50238-44. [2]Guo, JH, et al Rf-Activated Standing Surface Acoustic Wave for on-Chip Particle Manipulation. IEEE Transactions on Microwave Theory and Techniques.2014,09: 1898-904.
131 2018 International Conference of Biomedical Information Perception & Microsystem E.14 A Bio-inspired Airflow Directional Hair Sensor based on Quaternary Concentric FBGs Hong Li1, Lianqing Zhu 1* 1. Beijing Engineering Research Center of Optoelectronic Information and Instruments, Beijing Information Science and Technology University, Beijing, 100016, China Sensory hairs are common throughout the natural world, serving diverse functions in varied environments. For example, Crickets and many other orthopteran insects can detect an impinging airflow using mechanosensory hairs located on their cerci, as shown in Fig. 1. The traditional bionic airflow hair sensor are mainly based on the principle of capacitive or piezoresistive, whose sensing signals are susceptible to electromagnetic interference. Fiber optics is an approach to many applicaitons that is free from the constraints of electronic sensors. Important advantages of optical fiber sensors are that they are inherently small, electrochemically inert, and there is potential for multiplexing several sensors along a single optical fiber. Inspired by these filiform mechanosensory hairs, a bio-inspired airflow directional hair sensor based on quaternary concentric FBGs(QC-FBGs) was designed and fabricated, as shown in Fig. 1(a) and (b). A theoretical model of the 3D airflow sensor was developed -see Fig. 1(c), and the theoretical derivation and analysis were carried out.
Fig1. Sensing principle and experimental setup The sensing properties of the structure are investigated experimentally. Experimental results is shown in Fig. 1(d). The sensor is highly durable, capable of withstanding angular deflection exceeding 45 angular degrees in any direction. The optical signals of QC-FBGs were detected and analyed during the diferent speeds and directions of airflow in all 3-dimensions. The small scale of the sensor (~500μm footprint, ~0.2mg), sensitivity to low-speed air-flow, durability, and ease of fabrication and simple integration mark a new paradigm in small-scale optical hair sensor design and other bio-inspired artificial application.
References
[1] Flockhart G M H, Macpherson W N, Barton J S, et al. Two-axis bend measurement with Bragg gratings in multicore optical fiber[J]. Optics Letters, 2003, 28(6):387. [2] Bian Y, Zhang Y, Xia X. Design and Fabrication of a Multi-electrode Metal-core Piezoelectric Fiber and Its Application as an Airflow Sensor. Journal of Bionic Engineering, 2016, 13(3):416-425. [3] Maschmann M R, Ehlert G J, Dickinson B T, et al. Bioinspired Carbon Nanotube Fuzzy Fiber Hair Sensor for Air‐Flow Detection[J]. Advanced Materials, 2014, 26(20):3230-3234.
132 2018 International Conference of Biomedical Information Perception & Microsystem E.15 Precision Positioning System Based on Aerostatic Guide Drived by Ultrasonic Motor Li Bo1, Zhu Lianqing1* 1 Beijing Laboratory for Biomedical Detection Technology and Instrument, Beijing Information Science & Technology University, Beijing 100192, China *[email protected] Abstract With the development of the modern science and technology, the research of photoelectron, micro-electro-mechanical, bioengineering and nano materials in the field of micro is more and more deep, the demands of precision positioning is now into the micro and nano era. In order to realize precision positioning in high precision with wide-range, a precision positioning system with good repeatability is developed.The precision positioning system is consitested of the driver operative section and the measurement feedback section and the motion control section. The driver operative section comprises ultrasonic motor, linear guide and gravity balancing device.The motion control section comprises PMAC motion control card, computer and driver amplifier, etc.The measurement feedback section comprise the double-grating devices.Through repeative positioning accuracy experiment, the precise positioning system is tested, as the result of the experiment, the two-way repeated positioning accuracy is 0.1059μm.
References 朱玉龙. 基于双压电驱动宏微结合的纳米定位台研究[D]. 苏州：苏州大学，2013 Junhong Mao, Hiroyuki Tachikawa, Akira Shimokohbe. Precision Positioning ofa DC-motor-driven aerostatic slidesystem[J]. Precision Engineering, 2003, 27(1): 32-41. Peter Ekberg,Lars Stiblert,Lars Mattsson.A large-area ultra-precision 2D geometrical measurement technique based on statistical random phase detection[J]. Measurement Science and Technology, 2012, 23, (3):035007.
133 2018 International Conference of Biomedical Information Perception & Microsystem E.16 Microchannel structure design for cell capture by holographic array optical tweezers Liu Zhongsi1, Zhu Lianqing1* 1 Beijing Laboratory for Biomedical Detection Technology and Instrument, Beijing Information Science & Technology University, Beijing 100192, China *[email protected] Abstract Due to certain requirements of moving cells captured by optical tweezers, speed control of cell movement is necessary. In this paper, a flow focusing method is utilized to adjust cell movement state. A microchannel structure is designed for cell capture by holographic array optical tweezers. As the velocity of moving cell that can be captured by optical tweezers is limited, a microchannel structure is designed and simulated by COMSOL to control the velocity ratio of the sheath flow and the sample flow. By controlling the velocity ratio of the sheath and sample flow, the focus radius is changed. Three layers of liquid flow through the chamber, so that the cells were observed in the required speed range evenly through the chamber, making it easily captured by holographic array optical tweezers.
References Jennifer E. Curtis, Brian A. Koss, David G. Grier. Dynamic holographic optical tweezers [J]. Optics Communications 207, 2002, 169–175. Gwo-Bin Lee, Chih-Chang Chang, Sung-Bin Huang and Ruey-Jen Yang. The hydrodynamic focusing effect inside rectangular microchannels [J]. J. Micromech. Microeng. 16, 2006, 1024– 1032. An-Shik Yang · Wen-Hsin Hsieh. Hydrodynamic focusing investigation in a micro-flow cytometer [J]. Biomed Microdevices. 2007, 9:113–122.
134 2018 International Conference of Biomedical Information Perception & Microsystem E.17 Research on Mach-Zehnder Interferometric Sensing Characteristics Based on Seven-core Fiber and Spherical Symmetry Huaibao Li1, Xiaoping Lou2 * 1. Beijing Engineering Research Center of Optoelectronic Information and Instruments, Beijing Information Science and Technology University, Beijing, 100016, China 2. Optical Fiber Sensing and System Beijing Laboratory, Beijing Information Science and Technology University, Beijing, 100192, China In this paper, a simple, novel and cost-effective MZI sensor based on a symmetrical structure has been proposed by simply splicing a short segment of SCF and two fiber ball, as shown in Fig.1(a). The SCF acts as the sensor head, spliced between two single-mode fiber ball, forming a compact device with negligible insertion loss. It can be interrogated by either fringe visibility or wavelength peak shift. Its operating principle is based on the interference between the super-modes excited by the fundamental mode of input SMF. The transimission spectrum and FFT spacial result are shown in Fig. 1(b) and (c).
Fig1. Sensing principle and curvature temperature experimental result chart (a) Sensing structure and light propagation schematic (b)Relationship between curvature and center wavelength (c) Relationship between temperature and center wavelength The sensing properties of the structure are investigated experimentally. Experimental results is shown in Fig. 1(d) and Fig. 1(e),which show that the MZI sensor has a linear temperature sensitivity of ~58.97pm/℃ with a linearity of 0.99622. And the curvature sensitivity of the sensor is as high as 2.55nm/m-1 with a linearity of 0.99673 in the curvature range of 0~1 m-1. The wavelength of the transmission spectrum has a very good response to temperature and curvature. The sensor is characterized by all fiber structure, easy fabrication, structural stability and high sensitivity, which makes it attractive for communication and sensing application.
References
[4] Lin H S, Raji Y M, Lim J H, et al. Packaged in-line Mach–Zehnder interferometer for highly sensitive curvature and flexural strain sensing[J]. Sensors & Actuators A Physical, 2016, 250: 237-242. Li L, Li X, Xie Z, et al. All-fiber Mach-Zehnder interferometers for sensing applications[J]. Optics Express, 2012, 20(10):11109-11120. [5] Salceda-Delgado G, Van N A, Antonio-Lopez J E, et al. Compact fiber-optic curvature sensor based on super-mode interference in a seven-core fiber[J]. Optics Letters, 2015, 40(7):1468-1471.
135 2018 International Conference of Biomedical Information Perception & Microsystem E.18 Research on shape sensing fiber optic sensing method of biomimetic flexible antenna Liming Zhao1, Zhu Lianqing1,2 * 1. Beijing Engineering Research Center of Optoelectronic Information and Instruments, Beijing Information Science and Technology University, Beijing, 100016, China 2. Optical Fiber Sensing and System Beijing Laboratory, Beijing Information Science and Technology University, Beijing, 100192, China In order to solve the problem of the shape perception of bionic flexible antenna of intelligent robot, the flexible fiber sensing method was proposed and studied. Three-dimensional model of fiber tactile sensor was established as shown in Fig. 1(a) and (b). The formula of flexible antenna was deduced to detect objects in the shape of curvature. The flexible antenna shape sensing system was set up as shown in Fig. 1(c). In the experiment, the bending properties of the proposed bionic flexible antenna by the fiber grating wavelength shift and shape curvature monitoring was investigated.
Fig1. Sensing principle and experimental result chart The relationship between the parameters of the fitting curve and the actual curve between small deviation was obtained from the spectrum of the fiber sensors, whcih verified the feasibility of the bionic flexible antenna shape optical fiber sensing technology, as shown in Fig. 1(d) and (e). The results show that the optical fiber sensing method can realize the shape perception of bionic flexible antennas, which has the application prospect in the field of intelligent robot.
References [1] Yi Z, Zhang Y, Peters J. Bioinspired Tactile Sensor for Surface Roughness Discrimination. Sensors & Actuators A Physical, 2017, 255. [2] Zhao C, Jiang Q,Li Y. A novel biomimetic whisker technology based on fiber Bragg grating and its application. Measurement Science & Technology, 2017. [3] Jiang Q, Xiang L. Design and Experimental Research on Small-Structures of Tactile Sensor Array Unit Based on Fiber Bragg Grating. IEEE Sensors Journal, 2017, 17(7):2048-2054.
136 2018 International Conference of Biomedical Information Perception & Microsystem E.19 Raman probe fiber optimization based on CTO Jiabin Xia1,2, Guangkai Sun2, Lianqing Zhu2,1*, Mingli Dong2, Xiaoping Lou2 1School of Instrument Science and Opto-electronics Engineering, Hefei University of Technology, Hefei, 230009, China 2Beijing Enginerring Research Center of Optoelectronic Information and Instrument, Beijing Key Laboratory of Optoelectronics Measurement Technology, Beijing Information Science and Technology University,Beijing, 100192, China *Corresponding author: [email protected]
Abstract: For the treatment of chronic total occlusion(CTO), percutaneous coronary intervention(PCI) is one of the main treatments. In the current treatment process, only intravascular imaging technology can be provided, and the composition of each plaque in the blood vessel cannot be analyzed online. Intravascular analysis of plaque components is important for early diagnosis and clinical surgery. Here we use Raman probe spectroscopy to characterize intravascular plaque components. We designed three kinds of fibers(high-hydroxyl, low-hydroxyl, and pure-silica) to make Raman probes. The probe consists of six collection fibers and one excitation fiber. We use a 785nm laser as the excitation source, with cholesterol and triglycerides as the detection targets. Experiments explored the collection efficiency of different concentrations of cholesterol and triglycerides on different fibers. The full potential of Raman probe spectroscopy in the analysis of vascular plaque composition is fully demonstrated, providing an optimized solution for Raman probes.
References [1] Matthäus, Christian, et al. In Vivo Characterization of Atherosclerotic Plaque Depositions by Raman-Probe Spectroscopy and in Vitro Coherent Anti-Stokes Raman Scattering Microscopic Imaging on a Rabbit Model. Analytical Chemistry 84.18(2012):7845-7851. [2] Bergholt, M. S., et al. Fiber-optic Raman spectroscopy probes gastric carcinogenesis in vivo at endoscopy. Journal of Biophotonics 6.1(2013):49-59.
137 2018 International Conference of Biomedical Information Perception & Microsystem E.20 On-chip microparticle and cell washing using co-flow of viscoelastic fluid and Newtonian fluid Dan Yuan1, Say Hwa Tan2, Ronald Sluyter3,4, Qianbin Zhao1, Sheng Yan1, N. T. Nguyen2, Jinhong Guo5, Jun Zhang2,*, and Weihua Li1* 1School of Mechanical, Materials and Mechatronic Engineering, University of Wollongong, Wollongong, NSW 2522, Australia 2Queensland Micro- and Nanotechnology Centre, Griffith University, Brisbane, QLD 4111, Australia 3School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia 4Illawarra Health and Medical Research Institute, Wollongong, NSW 2522 Australia 5School of Electronic Engineering, University of Electronic Science and Technology of China, Chengdu 611731, China *Email: [email protected]. [email protected].
This work investigates the on-chip washing process of microparticles and cells using co-flow configuration of viscoelastic fluid and Newtonian fluid in a straight microchannel. By adding a small amount of biocompatible polymers into the particle medium or cell culture medium, the induced viscoelasticity can push particles and cells laterally from their original medium to the co-flow Newtonian medium. This behavior can be used for particle or cell washing. First, we demonstrated on-chip particle washing by the size-dependent migration speed using co-flow of viscoelastic fluid and Newtonian fluid. The critical particle size for efficient particle washing was determined. Second, we demonstrated continuous on-chip washing of Jurkat cells using co-flow of viscoelastic fluid and Newtonian fluid. The lateral migration process of Jurkat cells along the channel length was investigated. In addition, the cell washing quality was verified by hemocytometry and flow cytometry with a recovery rate as high as 92.8%. Scanning spectrophotometric measurements of the media from the two inlets and the two outlets demonstrated that diffusion of the co-flow was negligible, indicating efficient cell washing from culture medium to phosphate-buffered saline medium. This technique may be a safer, simpler, cheaper, and more efficient alternative to the tedious conventional centrifugation methods, and may open up a wide range of biomedical applications. Keywords: Viscoelastic fluid, viscoelastic force, cell washing, cell lateral migration.
138 2018 International Conference of Biomedical Information Perception & Microsystem E21 Achievement of nanostructured interfaces via flipping over electroless deposited metal electrodes for highly sensitive electronic skin ZhuanZhuan Shi,1 XiaoShuai Wu,1 ZhiSong Lu,1 Chang Ming Li,1 and Ling Yu1* 1 Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China *E-mail: [email protected] Abstract text Developments in electronic and miniaturization techniques, smart textiles, and flexible stretchable electronic skin (E-skin) have attracted tremendous interest due to their potential applications in wearable healthcare monitoring, sensitive tactile information gathering, and minimally invasive surgery1-3. Herein, a novel and simple method was developed to quickly pattern and transfer electrodes with nanostructures for fabricating flexible E-skin. A nano/micro-structure embedded Cu electrode can be fabricated from a solution process-based electroless deposition (ELD) on a frosted plastic substrate and subsequently flipped over with an adhesive tape. The fine nano/microstructures on the Cu layer benefit the pressure-electric response of the pressure sensor, demonstrated a high sensitivity: 2.22 kPa−1. This fabricated flexible E-skin can be used for monitoring human physiological signals, such as wrist pulse and thumb bending. This fabrication method is an economical tool for fast prototyping cost-effective wearable electronics for the detection and prediction of diseases. It offers opportunity for researchers from resource-limited laboratories to work on miniaturized wearable devices.
References 1. Rogers J A, et al. Materials and mechanics for stretchable electronics. Science. 2010, 327: 1603-1607. 2. Jeong G S, et al. Solderable and electroplatable flexible electronic circuit on a porous stretchable elastomer. Nat. Commun., 2012, 3: 977. 3. Wang X W, et al. Silk-Molded Flexible, Ultrasensitive, and Highly Stable Electronic Skin for Monitoring Human Physiological Signals. Adv. Mater., 2014, 26: 1336-1342.
139 2018 International Conference of Biomedical Information Perception & Microsystem E22 The tianxia120 『Medical - Drug - Inspection - Insurance』One-stop Digital Active health Follow-up Service Platform Birong dong1,#,Jinhong Guo2,#,Yuejun Kang2,#, Wenxue Wang2,#,Yongqiang Lin2,# ,Yonglin Ban3,#
1 Geriatric Center,West China Hospital of Sichuan University,Chengdu,610041, China 2 Chengdu Tianxia120 Co., Ltd., Chengdu,610041, China 3Chengdu Tianxiayisen Cloud-IoT Technology Co., Ltd. , Chengdu,610041, China *Email: [email protected] ,[email protected] ,[email protected] Chengdu Tianxia 120 Co., Ltd. integrates quality medical resource services in China's tertiary hospitals in its large health industry ecosystem, through its medical and health application scenarios, medical health mobile detection monitoring and intelligent hardware and medical applications based on medical Internet of Things technology applications. The ecological fusion solution formed by the multi-dimensional technology of healthy big data and medical artificial intelligence applies iterations, and is committed to providing users with leading “professional-active-continuous” intelligent digital medical health products and services, and creating “medical-drug-inspection-insurance” "One-stop active health management smart digital medical service ecosystem platform - [Tianxia 120]. Tianxia 120 digital active health service is guided by the multi-disciplinary consultation (MDT) systemic patient service theory. The top three expert team work model is the implementation of service units, together with intelligent mini digital medical diagnostic equipment, health data intelligent entry, health care Data processing and other systems allow users to enjoy “professional, proactive, and continuous” medical and health services from the top three hospital expert teams.
key point: 1, relying on intelligent digital technology as a support, provide active health services to individual users, and collect medical data on disease diseases; 2. Desensitize, clean up, dig, analyze and visualize medical big data, and at the same time “enable” the clinical research of the top three hospitals; 3, based on medical big data applied to a number of medical artificial intelligence model, continuous training, to explore more optimized multi-disease auxiliary diagnosis and disease prevention service solutions. References J. Guo, "Smartphone-Powered Electrochemical Biosensing Dongle for Emerging Medical IoTs Application," in IEEE Transactions on Industrial Informatics, vol. PP, no. 99, pp. 1-1. doi: 10.1109/TII.2017.2777145 J. Guo, Smartphone-Powered Electrochemical Dongle for Point-of-Care Monitoring of Blood β-Ketone, Analytical Chemistry, vol. 89, no. 17, pp. 8609-8613, 2017.
140 2018 International Conference of Biomedical Information Perception & Microsystem E23 Flexible Antenna Design on PDMS Substrate for Implantable Bioelectronics Applications Yusheng Fu*, Jianmin Lei , Xiao Zou *Email: [email protected] Abstract: In the current field of biomedical engineering, the research on implanted antennas has attracted more and more attention. Implantable antennas exist in the interior of the human body, and their role is to communicate wirelessly with external devices. However, for the traditional antenna, because of the complex application environment and some limitations, the embedded antenna that exists inside the body needs to meet the strict requirements of miniaturization, circular polarization, multiband, broadband, biological compatibility, and security. Therefore, this paper presents a flexible terrestrial radiating antenna with circular polarization characteristics that satisfies various requirements for biomedical implantable antennas. The new type of flexible material is adopted and a novel model is proposed. The square ground with small gap is implemented in the proposed antenna. The passive components can match the impedance and meet the requirements of the circular polarization wave. Simulation is carried out in a single layer tissue model to estimate the performance of the antenna and compared with the multilayer tissue model. In addition, the flexible circular polarized antenna has low profile characteristics, even if two coatings are less than 3mm, with a wide axial ratio bandwidth of 250MHz, which range from 2.28-2.53 GHz, and the antenna also shows good robustness to different implantation depth and the thickness of biocompatible coating. This paper uses traditional pork to simulate single layer and multi layer tissue model. The flexible circular polarized antenna prototype is placed in the organization model for performance simulation test, and the measurement impedance bandwidth of 620 MHz is realized in the industrial scientific medical frequency band of 2.4-2.48 GHz. Key words: flexible antenna, circular polarization, miniaturization, low profile, broadband
141 2018 International Conference of Biomedical Information Perception & Microsystem E24 PEGDA/PVP microneedles with tailorable matrix constitutions for controllable transdermal drug delivery Ya Gao , Mengmeng Hou, Ruihao Yang, Lei Zhang, Zhigang Xu, Yuejun Kang*, Peng Xue* Email: [email protected] (Y. Kang). [email protected] (P. Xue)
Abstract: Polymeric microneedles have attracted increasing interest for transdermal drug delivery owing to the unique advantages of minimal invasiveness, biocompatibility, biodegradability and efficient drug loading capacity. However, drug release from polymeric microneedles usually lacks controllability during transdermal administration. Herein, a poly(ethylene glycol) diacrylate/polyvinylpyrrolidone (PEGDA/PVP) microneedle patch with tailorable matrix constitutions was designed and fabricated using a two-step ultraviolet (UV) light-induced polymerization method. Scanning electron microscopic characterization revealed a non-uniform interior matrix of microneedles, resulting from the aggregation of PVP in the polymerized PEGDA matrix induced by UV light irradiation. The fabricated microneedles showed sufficient mechanical strength for skin penetration and could be removed intact after application. The controllable release property of rhodamine B (RhB)-loaded PEGDA/PVP microneedles was demonstrated in vitro by tuning the fraction of PVP content. The typical characteristics including initial burst and subsequent sustained release were attributed to the rapid dissolution of PVP and prolonged swelling of PEGDA, respectively. In addition, the microneedle insertion site could be completely recovered within one hour on the skin of a live mouse model, indicating the minimally invasive feature of microneedle-mediated therapeutic delivery. This scalable microneedle platform may serve as a convenient and effective drug carrier for transdermal delivery of various pharmaceuticals under precise control.
References [1] U. Wais, A. W. Jackson, T. He, H. Zhang, Nanoscale 2016, 8, 1746. [2] J. H. Park, M. G. Allen, M. R. Prausnitz. J. Control. Release 2005, 104, 51. [3] E. Larrañeta, M. T. C. McCrudden, A. J. Courtenay, R. F. Donnelly, Pharm. Res. 2016, 33, 1055.
142 2018 International Conference of Biomedical Information Perception & Microsystem E25 Accurate Electrochemical Blood Glucose Monitoring with a Smartphone by Adding Blood Cells Filtration Membrane
Yusheng Fu1, Haiyan Tan2, Jinhong Guo3 *Email: [email protected]
Abstract: We report a novel blood glucose (BG) testing technology intergraded with a new electrochemical biosensor by adding blood cell filtration membrane in conventional strip. The membrane could filtrate blood cells and eliminate the inaccurate results generated by the blood cells in samples. When the peripheral blood dropped on test strip, it reacts with glucose oxidase (GOD) on the bars. There are linear correlation among the level of blood glucose and electrical current produced in the process of electrochemistry testing. Therefore, the concentration of blood glucose could be measure with the current signal produced from the reaction. A wireless device dongle is applied to process sample signals data and connect with smartphone. The evaluating results shows in a smartphone application called Great Health. Patients monitor their condition of health and sought assistance on-line or appointment with doctor in it. The accuracy and precision of the proposed BG biosensor is estimated with a revised edition regulation ISO15197:2013. The results illustrate that the method agrees with the requirements about accuracy and the cost is much lower than the clinical biochemical analyser with dongle and the novel test strip. The system is portable, cost-effective, accurate and fit for Point of Care (POC).
[1]Reichard P, Nilsson BY, Rosenquist U (1993) The effect of long-term intensified insulin treatment on the development of microvascular complications of diabetes mellitus. The New England journal of medicine 329:304 [2]Rosenqvist U (1993) The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. The Diabetes Control and Complications Trial Research Group. New England Journal of Medicine 329:977-986 [3]Shaw JE, Sicree RA, Zimmet PZ (2010) Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Research & Clinical Practice 87:4-14
143 2018 International Conference of Biomedical Information Perception & Microsystem E26 Numerical Modeling of Impedance-based Lab-in-a-Tube Chip for Biofluidsnalysis Analyses Yusheng Fu1, Hong Ji *Email: [email protected] Abstract: Human blood is a kind of critical and direct biosample to diagnose diseases. The physical, electronic, chemical and biological characteristics of human blood work as pivotal methods to detect specific diseases. The physical and electronic feature of human blood, mainly including electrical conductivity, relative permittivity and frequency characteristic, is one of important issue in the medical and health fields, which are widely used in the manufacture of various biomedicine instruments. In this paper, we demonstrate a numerical modeling based on impedance spectroscopy method. The numerical model utilizes a pair of electrodes encircled a glass tube filling with the blood sample to estimate its electronic and physical properties. The resistance of human blood is approximately obtained by the model, which has commercial and biological value for illness diagnoses. The numerical model is constructed by the software COMSOL Multiphysics, and each electronic component in the numerical modeling equivalent circuit is fitted by EIS Spectrum Analyser. Index Terms: human blood, physical and electronic feature, numerical modeling, electrodes, resistance.
144 2018 International Conference of Biomedical Information Perception & Microsystem E27 The Realization of Pulsed-Wave Doppler Spectrum Estimation Module Lin Chen1, Lei Liu1, Quanyong Wang1, Zhe Wu1* 1 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China *Email: [email protected]
Abstract: At present, cardiovascular and cerebrovascular diseases are the leading cause of death in the world. Since ultrasound Doppler technology can detect the blood flow in human body in a non-invasive and real-time manner, it has been widely used as an important technique for clinical diagnosis of cardiovascular and cerebrovascular diseases. Estimating the spectrum of pulsed Doppler ultrasound echo signals can effectively extract information such as the blood flow velocity, blood flow direction, and pulsation index. Changes in blood flow velocity due to lesions provide important clinical diagnostic evidence in the examination of vascular diseases. Therefore, it is necessary to perform real-time processing and spectrum estimation to obtain the Doppler signal of blood flow information effectively and accurately. Based on the characteristics of human vessels and blood flow, we have introduced the principle and corresponding methods of pulse wave Doppler ultrasound blood flow detection. Then the ultrasonic control platform is used to control the transmission and reception of pulsed wave Doppler ultrasound signals. Finally, the digital signal processing methods such as wall filter and short-time Fourier transform (STFT) are used to analyse the characteristics of echo signals from the spectrum in frequency domain, and the diagnostic parameters such as speed and direction of blood flow signals are estimated.
Keywords: pulsed wave Doppler ultrasound; Spectrum Estimation; STFT
Reference: 1. Shaw R F, Lambert L B. PULSED DOPPLER VOLUMETRIC BLOOD FLOWMETER:, US3498290[P]. 1970. 2. Reddy A K, Jones A D, Martono C, et al. Pulsed Doppler signal processing for use in mice: design and evaluation[J]. IEEE Transactions on Biomedical Engineering, 2005, 52(10):1764-1770.
145 2018 International Conference of Biomedical Information Perception & Microsystem E28 A Smartphone-Powered Photochemical Dongle Based on Test Strip for Remote Uric Acid Monitoring Yusheng Fu1 , Huan Yang2, Xiwei Huang3, Jinhong Guo4 *Email: [email protected]
Abstract A simple and convenient photochemical system based on smartphone-powered photochemical dongle and disposable photochemical test strips was proposed in this paper.They were only connected with each other in the simplest hot-plug way, but provided a prominent function of biological sample detection that was beyond the imagination. The photochemical dongle worked as a highly rigorous reflectance spectral analyzer was used to evaluate the Uric Acid levels of the fingertip whole blood with the participation of the photochemical test strip, which showed good agreement (linear regression coefficient of 0.99338) as compared to the results from the specific and bulky biochemical analyzer in the clinical test for the Point of Care. What was more,combined with the widespread smartphone and well-developed Internet and made the most of them consequently, the photochemical dongle could provide a flexible and portable platform for the evaluation and treatment of chronic diseases like gout is promising to be applied in the remote chronic disease management.
[1]J. S. Cameron, F. Moro, and H. A. Simmonds, "Gout, uric acid and purine metabolism in paediatric nephrology," Pediatric Nephrology, vol. 7, no. 1, pp. 105-118, Feb. 1993, DOI. 10.1007/BF00861588 . [2]B. Álvarez-Lario, J. Macarrón-Vicente, "Uric acid and evolution," Rheumatology, vol. 49, no. 11, pp. 2010-2015, Jul. 2010, DOI. 10.1093/rheumatology/keq204. [3]H. Iwahana, M. Itakura, "Inherited disorders of uric acid metabolism--classification, enzymatic- and DNA-diagnosis," Nihon Rinsho, vol. 54, no. 12, pp. 3303-3308, Dec. 1996. Wang LM, et al. Selective Targeting of Gold Nanorods at the Mitochondria of Cancer Cells: Implications for Cancer Therapy. Nano lett. 2011, 11:772-780. (Times New Roman 10 lbs, single spacing, justify)
146 2018 International Conference of Biomedical Information Perception & Microsystem
147 2018 International Conference of Biomedical Information Perception & Microsystem
重庆迈联医疗科技有限公司
血脂、血糖、尿酸、总胆固醇、血酮多功能检测仪（Fast Analyzer）
公司简介
重庆迈联医疗科技有限公司是由重庆两江新区引进海外归国博士团队于 2018 年成立的，专业从事体外诊断试剂和医学 检测仪器研发，生产，销售的高科技企业。公司目前自主研发多款产品，拥有多项发明与实用新型专利。目前产品覆盖血 糖，尿酸，血酮体，总胆固醇，高密度脂蛋白胆固醇，低密度脂蛋白胆固醇，甘油三酯等检测项目。其中集成的干电化学手 持式检测仪填补了国内移动检验终端的空白。配套试纸条技术处于国内领先水平。
目前已建成符合 CFDA 医疗器械生产体系要求的生产基地，拥有体外诊断试剂及其配套仪器生产的十万级 GMP 洁净生 产车间。公司严格执行体外诊断生产条例实施生产，已建立完善的生产与质控体系。
产品简介
仪器的型号：Fast Analyzer
产品特点
1、 集成干化学与电化学测量功能，支持多种检测项目；
总胆固醇、低密度脂蛋白、高密度脂蛋白、甘油三酯
血糖、尿酸、总胆固醇、血酮
2、采用点阵屏，可显示丰富的测量结果和统计图表；
3、集成蓝牙、TTL 通讯接口，方便数据与上位机通信；
4、采用高精密器件、独创的遮光盖设计大幅提高测量精度；
5、内置锂电池；
6、CODE 卡与 USB 端口融合设计，提高通用性；
7、具备标准 Tpye-c 接口；
性能参数 外部供电： usb 直流 5V 内部锂电池: 600mah 通讯功能：蓝牙、TTL 计量单位：mmol/L
仪器尺寸：130mmX65mmX20mm 显示屏：128x128 点阵屏 工作温度：15-35°C 工作湿度：<80%
公司名称：重庆迈联医疗科技有限公司 地址：重庆市北碚区丰和路 110 号 2 楼
联系人:张老师 电话:15928165789 邮箱:[email protected] 公司网站：www.gotechcn.cn
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