Structure and Substrate Specificity of Heparinase III from Bacteroides Th
Glycobiology, 2017, vol. 27, no. 2, 176–187 doi: 10.1093/glycob/cww096 Advance Access Publication Date: 12 October 2016 Original Article Structural Biology Conformational flexibility of PL12 family heparinases: structure and substrate specificity of heparinase III from Bacteroides thetaiotaomicron (BT4657) ThirumalaiSelvi Ulaganathan2, Rong Shi3, Deqiang Yao4, Ruo-Xu Gu5, Marie-Line Garron6, Maia Cherney2, D Peter Tieleman5, Eric Sterner7, Guoyun Li7, Lingyun Li7, Robert J Linhardt7, and Miroslaw Cygler2,1 2Department of Biochemistry, University of Saskatchewan, Saskatoon, S7N 5E5 Saskatchewan, Canada, 3Département de Biochimie, de Microbiologie et de Bio-informatique, PROTEO, and Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Pavillon Charles-Eugène-Marchand, Québec City, QC G1V 0A6, Canada, 4National Center for Protein Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China and Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai 201204, China, 5Department of Biological Sciences and Centre for Molecular Simulation, University of Calgary, 2500 University Dr NW, Calgary, AB T2N 1N4, Canada H4P 2R2, Canada, 6the Architecture et Fonction des Macromolécules Biologiques, UMR7257 CNRS, Aix-Marseille University, F-13288 Marseille, France, the INRA, USC1408 Architecture et Fonction des Macromolécules Biologiques, F-13288 Marseille, France, and 7Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA 1To whom correspondence should be addressed: Tel: +1 (306) 966-4361, Fax: +1 (306) 966-4390; e-mail: miroslaw.cygler@usask.ca Received 1 July 2016; Revised 5 September 2016; Accepted 6 September 2016 Abstract Glycosaminoglycans (GAGs) are linear polysaccharides comprised of disaccharide repeat units, a hexuronic acid, glucuronic acid or iduronic acid, linked to a hexosamine, N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine.
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