Essais Botaniques Sur Certaines Plantes Non Toxiques Utilisées En

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Essais Botaniques Sur Certaines Plantes Non Toxiques Utilisées En الجـــمـــهوريـــــة الجزائريـــة الديمقــــــراطيــة الشــــعـــبية République Algérienne Démocratique et Populaire وزارة الــــــــــتعـــليم العــــالــــــــــــي MINISTERE DE والبـــحث العـــــــــــــــلمـــــــــي L’ENSEIGNEMENTSUPERIEUR ET DE LA RECHERCHE SCIENTIFIQUE UNIVERSITE ABOU BEKR جــامعة أبو بكــر بلـقـا يد BELKAÎD كـليــة الطب د. ب. بن زرجــب – تلمســان FACULTE DE MEDECINE D R. B. BENZERDJEB - TLEMCEN DEPARTEMENT DE PHARMACIE MEMOIRE DE FIN D’ETUDES POUR L’OBTENTION DU DIPLOME DE DOCTEUR EN PHARMACIE THÈME : Essais botaniques sur certaines plantes non toxiques utilisées en médecine traditionnelle dans la région de Tlemcen (Apiacées, Astéracées, Lamiacées) Présenté par : MEDJAHED HASNIA BADRIA YAZID KHAWLA Soutenu le : 07/07/2019 Le Jury Président : Pr. BEKHCHI (Professeur en biologie) Université de Tlemcen. Membres : Dr. S. BABA AHMED (Maitre Assistante en Pharmacognosie) Université de Tlemcen. Dr. M. TABTI (Assistant en Botanique Médicale) CHU Tlemcen. Encadreur : Dr. S. HASSAINE (Maitre Assistante en Pharmacognosie) Université de Tlemcen. Co-encadreur : Dr. N. ELYEBDRI (Maitre Assistante en Pharmacognosie) Université de Tlemcen. Année Universitaire 2018-2019. REMERCIEMENTS : Avant toute chose, nous remercions Allah qui nous a donné la force et la patience pour faire aboutir ce travail. Nous adressons nos remerciements : À Docteur S. HASSAÏNE, maitre assistante en pharmacognosie département de pharmacie Tlemcen, pour son encadrement, ses précieux conseils et sa patience qui nous a aidé à réaliser ce travail. À Docteur N. EL YEBDRI, maitre assistante en pharmacognosie département de pharmacie Tlemcen, pour son Co-encadrement, et pour son aide. À Mme CH. BAKHECHI, Professeur en biologie d’avoir accepté d’assurer la présidence du jury de notre mémoire de fin d’études. À Docteur S. BABA AHMED, maitre assistante en pharmacognosie, d’avoir accepté d’examiner ce modeste travail. À Docteur M. TABTI, assistant en botanique médical, pour avoir accepté d’examiner ce modeste travail en faisant partie du jury. Aux joyaux de notre vie « nos parents » qui sont la source de toutes réussites, nous souhaitons qu’ils trouvent à travers ce mémoire le faible témoignage de leurs efforts et sacrifices. I DEDICACE : Je voudrais dédier cet humble travail : À ma chère mère, qui m’a donné tout, l’amour, l’envie de vivre, l’encouragement, ainsi que pour sa patience et son soutien tout au long de mes études. À ma chère grand-mère et mon cher grand père. À mon oncle AHMED. « رحمه هللا» À la mémoire de mon oncle MOHAMMED Qui me manque énormément, que Dieu tout puissant l’accueille dans son vaste paradis. À mon frère MOHAMMED EL AMINE, je suis très heureuse qu’il soit dans ma vie. À ma sœur KHEIRA qui m’a soutenue dans les mauvais moments de ma vie. À mon petit frère ABDALLAH à qui je souhaite tout le bonheur de la vie. À ma tante FATIMA qui est ma deuxième mère. À mes cousines FADIA, AMINA, ASMA A mes cousins IMED et TAHA. À tous mes amis et particulièrement LAISSOUF SOUHILA qui est ma deuxième sœur, qui m’a beaucoup aidée dans la réalisation de ce travail. À mes tantes et mes cousines et cousins chacun en son nom. À ceux qui ont participé de près ou de loin dans la réalisation de ce travail. Et à tous ceux que j’aime. YAZID KHAWLA II Tous d’abord, je dédie ce travail à mes chers parents, pour leurs رحمها هللا sacrifices et leur soutien, spécialement à ma mère À toute ma famille, la famille KEBIR surtout, à ma grand-mère et mes frères Imad et Abdelkader surtout. À mes chères amies : un remerciement spécial à ma chère Laissouf Souhila, aussi à Houda, Khadidja pour leur aide et pour les moments qui resteront inoubliables pour nous, au cours de mon cursus à l’université. رحمها هللا À ma tante Zahia À toute personne qui m’a aidé de près ou de loin. Sans vous, Sans votre soutien moral, Sans votre amour, Rien n’aurait été possible. MEDJAHED HASNIA- BADRIA III SOMMAIRE IV TABLES DES MATIERES : REMERCIEMENT………………………………………………………………...…………………….I DÉDICACE…….………………………………………………………..………………………………..II LISTE DES ABRÉVIATIONS…………………………………….……………………………………IX LISTE DES FIGURES………………………………………………………………...……….…………X LISTE DES TABLEAUX………………………………………………...……………...…….………XIX INTRODUCTION………………………………………………………………………….………...……1 PREMIERE PARTIE : ETUDE BIBLIOGRAPHIQUE…………………………………..……...…….3 CHAPITRE I : GÉNÉRALITÉS SUR LA PHARMACOGNOSIE…………….………………………4 1. Définition de la pharmacognosie………………….………………….………………………………….5 2. Terminologie……………………………………………..…………………………………………….…5 2.1 Notion de plante médicinale………………………..…………………………………………….5 2.2 Notion d’une drogue végétale………………………………..…………………………………...5 2.3 Notion d’un principe actif…………………………………………….………………………….6 3. Récolte……………………………………………………………………..……………………………...6 4. Conservation et stockage…………………………………..…………………………………………….6 5. Contrôle……………………………………………………………….………………………………….7 6. Normalisation…………………………………………………………………………………………….8 6.1 Essais botaniques…………...……………………………………………………………………8 6.2 Essais physicochimiques……..…………………………………………………………………..9 6.3 Essais biologiques………………………..……………………………………………………….9 7. Monographie d’une plante médicinale………..………………………………………………………...9 V 7.1 La dénomination botanique………...……………………………………………………………9 7.2 Les caractères botaniques. ……………………………………...……………………………...10 7.3 La composition chimique…………………………...…………………………………………..10 7.4 Les propriétés pharmacologiques………………..……………………………………………..10 7.5 L’usage traditionnel…………………………….……………………………………………….10 CHAPITRE II : GÉNÉRALITÉS SUR L’HISTOLOGIE VÉGÉTALE…………………………...…11 1. L’histologie des tissus végétaux……………….…………………………………………………...…12 2. Les tissus végétaux……………………………..……...………………………………………………12 2.1 Les tissus méristématiques…………………………………………….……………………….12 2.2 Les tissus de protection…………….…………………………………….……………………..13 2.3 Les tissus de nutrition……………….……………………………….…………………………13 2.4 Les tissus conducteurs……………………………………………..…………………………....14 2.5 Les tissus de soutien………………………………………………………..……………………15 2.6 Les tissus sécréteurs…………………………………………………………….……………....16 CHAPITRE III : MONOGRAPHIES DES PLANTES ÉTUDIÉES…………………………………..18 1. Famille des Apiacées…………………...………………………………………………..…………….20 1.1 Le céleri……………..………………………...……………………………………………........25 1.2 La coriandre…………………………………………...………………………………………..27 1.3 Le cumin……...…………………………………………………………………………………31 1.4 Le cumin velu…………………...………………………………………………………………34 1.5 Le fenouil…………..……………………………………………………………………………35 1.6 Le persil………………………..………………………………………………………………..38 VI 2. Famille des Astéracées……………………..………………………………………………………….42 2.1 L’inule visqueuse…………………...…………………………………………………………...45 2.2 Le pyrèthre d’Afrique…………………………………………………………………………..48 2.3 Le scolyme d’Espagne…………………………………………………………………………..52 3. Famille des Lamiacées………………………………………………………………………………...55 3.1 Le basilic………………………………………………………………………………………...58 3.2 La lavande stoechade…………………………………………………………………………...60 3.3 La lavande vraie………………………………………………………………………………...62 3.4 La marjolaine…………………………………………………………………………………...64 3.5 Le marrube blanc……………………………………………………………………………….67 3.6 La menthe poivrée……………………………………………………………………………....69 3.7 La menthe pouliot……………………………………………………………………………….72 3.8 La menthe ronde………………………………………………………………………………...75 3.9 La menthe verte…………………………...…………………………………………………….78 3.10 L’origan……………………..………………………………………………………………….80 3.11 Le romarin……………………………………………...………………………………………84 DEUXIEME PARTIE : PARTIE EXPÉRIMENTALE……………………………………………….88 CHAPITRE I : MATÉRIEL ET MÉTHODES…………………………………………..……………..89 CHAPITRE II : RÉSULTATS ET DISCUSSION……………………………………….…….……….95 DESSINS ET SCHEMAS ILLUSTRATIFS DES COUPES TRANSVERSALES ET DES POUDRES DES PLANTES ETUDIEES……………………………………………………………………………192 VII CLE D’IDENTIFICATION BOTANIQUE DES DROGUES DE NOTRE ETUDE………………..242 CONCLUSION…………………………………………………………………………………………243 REFERENCES………………………………………………………………………………………….245 ANNEXE……………………………………….……………………………………………………….258 GLOSSAIRE……………………………………………………………………………………………260 VIII LISTE DES ABREVIATIONS : A.F.N.O.R : l’Association Française de Normalisation. AO : l'Acide Okadaïque. CCM : Chromatographie sur Couche Mince. CE : Commission Européenne. CLHP : Chromatographie en Phase Liquide à Haute Performance. CLR : Coupe Longitudinale Radiale. CLT : Coupe Longitudinale Tangentielle. CT : Coupe Transversale. DL50 : Dose Létale Médiane. DMM : Dose Minime Mortelle. DPPH 2-2 diphényle picrylhyrazyl. FLL : Faisceau Libéro-ligneux. HDL : les Lipoprotéines de Haute Densité. ISO : Organisation Internationale de normalisation. LDL : les Lipoprotéines de Basse Densité. OMS : Organisation Mondiale de Santé. ORAC : Oxygen Radical Absorbance Capacity. PA : Principe Actif. TAC : Total Antioxydant Capacity. VLDL : Lipoprotéine de très basse densité. IX LISTES DES FIGURES : Figure 1 : Mode de classification classique des êtres vivants……………………………………….............9 Figure 2 : Les différents types de collenchyme…………………….……...………………………………15 Figure 3 : coupe transversale de la feuille de : Convallaria majalis L. (Liliacées) (la nervure centrale) montrant le faisceau libéro- ligneux et le sclérenchyme (en vert) ……………………..…………………….15 Figure 4 : Quelques types de poils sécréteurs……………………………………………………………...16 Figure 5 : Coupe transversale d'une feuille d’Eucalyptus globulus L. (Myrtacées)……………………..…17 Figure 6 : Canal sécréteur d’Anacyclus pyrethrum L. (Astéracées) ……………………………………….17 Figure 7 : Figure montrant l’aspect et les schémas des coupes transversales de quelques fruits d’Ombellifères…...………...………………………………………………………………………………..23 Figure 8 : Observations microscopiques de la coupe transversale du fruit
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