Diacetyl Take a Single Short Sniff

beer flavor standard 2,3-butanedione OFF-FLAVOR KIT available from www.cicerone.org

ASSESSMENT CONFUSIONS Without covering the glass, swirl • Butyric acid the beer to release the aroma. • Vanillin Diacetyl Take a single short sniff. Repeat • Isobutyraldehyde “like butter, or as necessary. IMPORTANCE butter popcorn” THRESHOLD stouts 10 – 40 µg/l and lagers, eg in other lager beers. Considerable ORIGINS efforts are made by breweries to Produced in beer from a precursor formed by yeast during fermentation. Can also be formed REMARKS by contaminant lactic acid bacteria. 2,3-Butanedione is one of two vicinal diketones found in beer. O

The ratio of diacetyl to CH3 pentanedione concentrations can H3 C be used as an indicator of bacterial contamination in beer.

TION O

% POPUL A RANDALLS ROAD LEATHERHEAD, SURREY FLAVOR THRESHOLD KT22 7RY, UK beer flavor standard dimethyl sulfide OFF-FLAVOR KIT available from www.cicerone.org

ASSESSMENT CONFUSIONS Without covering the glass, swirl • Methyl thioacetate the beer to release the aroma. • Ethanethiol DMS Take a single short sniff. Repeat • Dimethyl trisulphide “like sweetcorn or as necessary. IMPORTANCE tomato sauce” THRESHOLD 30 - 50 µg/l beers. Excessive levels are indicative ORIGINS of growth of contaminant bacteria Formed from malt-derived during fermentation. precursors, primarily during wort production and – to a lesser extent – REMARKS during fermentation. The perception of dimethyl sulfide H3 C CH3 aromatic higher alcohols such as 2- S phenylethanol in beer.

CAS NUMBER TION

75-18-3 www.aroxa.com [email protected]

papery acetaldehyde trans-2-nonenal acetaldehyde

lightstruck diacetyl 3-methyl-2-butene-1-thiol 2,3-butanedione

infection DMS diacetyl dimethyl sulphide and acetic acid

control

beer flavor standard acetaldehyde OFF-FLAVOR KIT available from www.cicerone.org

ASSESSMENT CONFUSIONS Cover the beer with your hand and • Ethyl hexanoate swirl the glass to release the aroma. • 2,4,6-Trichloroanisole Acetal- Remove your hand and take a single • cis-3-Hexenol long sniff. Repeat as necessary. dehyde IMPORTANCE “like emulsion THRESHOLD Present in all beers. Characteristic 5 mg/l paint or green eg apples” ORIGINS at high concentrations. Produced by yeast during fermentation. Indicative of REMARKS fermentation problems and poor control of dissolved oxygen in

packaging. concentration in the beer. 3 C O

CAS NUMBER 75-07-0 TION

www.aroxa.com [email protected]

ASSESSMENT CONFUSIONS Best detected by smelling the beer • Sulphur dioxide at a distance. Hold the glass at • cis-3-Hexenol Light- arm’s length and take short sniffs to • Ethanethiol prevent the risk of adaptation. struck IMPORTANCE THRESHOLD “like a skunk or 4 – 30 ng/l to light of beer packaged in clear freshly brewed or green glass. Consumers are very ORIGINS coffee” Formed as a result of exposure of at the point of consumption. This initiates a reaction involving hop bitter acids, vitamins and REMARKS CH3 sulphur compounds. Small amounts Beers which have been bittered with are also formed in beer production. not develop this character.

CH3 CAS NUMBER

TION SH 5287-45-6 www.aroxa.com [email protected]

% POPUL A RANDALLS ROAD LEATHERHEAD, SURREY FLAVOR THRESHOLD KT22 7RY, UK beer flavor standard trans-2-nonenal OFF-FLAVOR KIT available from www.cicerone.org

ASSESSMENT Without covering the glass, swirl CONFUSIONS the beer to release the aroma. Papery Take a single short sniff. Repeat • 2,4,6-Trichloroanisole “like cardboard as necessary. • 2-Ethyl fenchol • Isobutyraldehyde or oxidized beer” THRESHOLD IMPORTANCE 50 – 250 ng/l

ORIGINS Formed in malt and wort production more pronounced when precautions where it binds to malt proteins. have not been taken in relation to Carried through the brewing minimizing process oxidation. process in this bound form. REMARKS Sulphur dioxide in beer reversibly beer in pack. CH3 reacts with trans-2-nonenal to O

TION CAS NUMBER www.aroxa.com 18829-56-6 [email protected]

ASSESSMENT CONFUSIONS Without covering the glass, swirl • Acetic acid (Vinegar) the beer to release the aroma. • Diacetyl Infection Take a single short sniff. Repeat as necessary. IMPORTANCE “like bile, or THRESHOLD This combination of flavors stomach acid.” Different for each component indicates poor draft system sanitation. INFECTION: ORIGINS Components are Improperly cleaned draft lines can acetic acid, citric acid harbor bacteria that release and 2,3-butanedione several off-flavors into beer. REMARKS This standard includes multiple flavors: acetic acid, 2,3- butanedione and citric acid. TION

www.aroxa.com [email protected]

% POPUL A RANDALLS ROAD LEATHERHEAD, SURREY FLAVOR THRESHOLD KT22 7RY, UK