DOCSLIB.ORG

Complete Mitochondrial Genome of Concholepas Concholepas Inferred by 454 Pyrosequencing and Mtdna Expression in Two Mollusc Populations

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Complete Mitochondrial Genome of Concholepas Concholepas Inferred by 454 Pyrosequencing and Mtdna Expression in Two Mollusc Populations Comparative Biochemistry and Physiology, Part D 8 (2013) 17–23 Contents lists available at SciVerse ScienceDirect Comparative Biochemistry and Physiology, Part D journal homepage: www.elsevier.com/locate/cbpd Complete mitochondrial genome of Concholepas concholepas inferred by 454 pyrosequencing and mtDNA expression in two mollusc populations Gustavo Núñez-Acuña, Andrea Aguilar-Espinoza, Cristian Gallardo-Escárate ⁎ Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research, University of Concepción, PO Box 160-C, Concepción, Chile article info abstract Article history: Despite the great relevance of mitochondrial genome analysis in evolutionary studies, there is scarce information Received 2 August 2012 on how the transcripts associated with the mitogenome are expressed and their role in the genetic structuring of Received in revised form 20 October 2012 populations. This work reports the complete mitochondrial genome of the marine gastropod Concholepas Accepted 21 October 2012 concholepas, obtained by 454 pryosequencing, and an analysis of mitochondrial transcripts of two populations Available online 27 October 2012 1000 km apart along the Chilean coast. The mitochondrion of C. concholepas is 15,495 base pairs (bp) in size and contains the 37 subunits characteristic of metazoans, as well as a non-coding region of 330 bp. In silico anal- Keywords: fi Mitochondrial genome ysis of mitochondrial gene variability showed signi cant differences among populations. In terms of levels of rel- Concholepas concholepas ative abundance of transcripts associated with mitochondrion in the two populations (assessed by qPCR), the RNA-Seq genes associated with complexes III and IV of the mitochondrial genome had the highest levels of expression Gene expression in the northern population while transcripts associated with the ATP synthase complex had the highest levels of expression in the southern population. Moreover, fifteen polymorphic SNPs were identified in silico between the mitogenomes of the two populations. Four of these markers implied different amino acid substitutions (non-synonymous SNPs). This work contributes novel information regarding the mitochondrial genome struc- ture and mRNA expression levels of C. concholepas. © 2012 Elsevier Inc. All rights reserved. 1. Introduction reported that there is a noncoding region in several species that can play a regulatory role in the duplication and transcription of the mito- In general, the genomic organization of the mtDNA in marine organ- chondrial genome, although it is difficult to study its homology in higher isms have been studied through long-PCR and random genome sequenc- taxa because it presents similarities only among closely related taxa ing with DNA nebulization and the creation of clone libraries with pUC or (Passamonti et al., 2011). Complete mitochondrial genome analysis in- Bluescript vectors (Burger et al., 2007). However, recent advances in creases the resolution of works on the molecular phylogeny of species, next-generation sequencing technology (NGS) have increased the veloc- allowing the identification of genetic reordering among the higher taxa ity of mitochondrial genome characterization of several vertebrate and of species (Rawlings et al., 2010). Mitochondrial genome analysis signifi- invertebrate marine species (Jex et al., 2008; Neira-Oviedo et al., 2011). cantly increases the resolution of philogenetic studies of lower taxa, com- The main advantage of using NGS platforms is the possibility of using bining the data of all the genes in a single set (Cunha et al., 2009). Finally, the data generated to identify specific genes and to infer its relative intraspecific analysis of the mitochondrial genome evidences possible abundance under different conditions of study (Jex et al., 2008; Neira- mtDNA polymorphism (single nucleotide polymorphisms, SNPs) associ- Oviedo et al., 2011), which is of particular interest in comparative geno- ated with different geographic locations (Xin et al., 2011). Nevertheless, mic ecology studies. to our knowledge there have been no studies relating mtDNA polymor- The mitochondrial genome is a macromolecule with a size that in the phism to patterns of mitochondrial gene expression at the biogeographic majority of metazoans ranges between 15 and 20 kb (Boyce et al., 1989; level. In this context, important questions remain unanswered, such as: Is Boore, 1999). In practically all metazoans the mitochondrion is circular, it possible to find local patterns of mitochondrial gene expression? Does except in some classes of cnydaria that have lineal mitochondrial DNA variation of mitochondrial gene expression imply local adaptations? chromosomes (Bridge et al., 1992). The mitochondrial genome generally Our model of study, Concholepas concholepas, is a marine gastropod has 13 enzymatic protein subunits involved in the process of oxidative found along the Chilean coast. It has become a commercially important phosphorylation, 22 tRNA and 2 ribosomal subunits. As well, it has been benthic resource for Chile (Leiva and Castilla, 2001). One of the main characteristics of these species at the ecological level is their long larval development stage in the water column, which can last from almost ⁎ Corresponding author at: Interdisciplinary Center for Aquaculture Research, Univer- three months to one year. This extensive stage of planktonic develop- sity of Concepción, PO Box 160-C, Concepción, Chile. Tel.: +56 41 2203422; fax: +56 41 2207310. ment results in a broad geographic distribution of the species from 6°S E-mail address: [email protected] (C. Gallardo-Escárate). to 56°S. Genetic studies based on the coding region of the cytochrome 1744-117X/$ – see front matter © 2012 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.cbd.2012.10.004 18 G. Núñez-Acuña et al. / Comparative Biochemistry and Physiology, Part D 8 (2013) 17–23 oxidase I gene (COX1) do not show genetic-population structuring in accordance with the supplier's instructions. The RNA was purified among different C. concholepas groups over the range of their biogeo- and the concentration and purity were measured with a spectropho- graphic distribution (Cárdenas et al., 2009). Nevertheless, an unan- tometer (ND-1000, Nanodrop Technologies) and the integrity was swered question is whether there are genes in C. concholepas that visualized with electrophoresis in MOPS/formaldehyde agarose gels at show highly differentiated biogeographic patterns due to environmen- 1.2% staining with ethidium bromide at 0.001%, and then quantified tal factors (e.g. northern and southern Chile). New DNA molecular with a 2100 bioanalyzer (Agilent Technologies, USA). Selected extrac- markers have recently been described for C. concholepas as micro- tions presented 260/280 and 260/230 purity indices equal to or greater satellites (Cárdenas et al., 2006) and ESTs obtained by 454 pyro- than 2.0 and showed integral RNA in electrophoresis and bioanalyzer sequencing (Cárdenas et al., 2011). As well, there are now candidate measurements (RIN>8). Subsequently, sample pools of selected RNAs genes related to thermal, oxidative and immune responses that allow were prepared (one pool for each population group) and precipitated for testing hypotheses about local adaptation at the genomic level. overnight with 2× volumes of absolute ethanol and 0.1× volume of so- The objective of the present study was to describe for the first dium acetate 0.3 M at −80 °C. Double-stranded cDNA was synthesized time the mitochondrial genome for C. concholepas using 454 cDNA from the extracted RNA and pyrosequenced (full plate) in a 454 GS FLX pyrosequencing (RNA-seq). As well, we evaluated the levels of titanium platform by Macrogen Inc. (S. Korea). gene expression (in silico analysis) of all the mitochondrial genes in The raw data for both samples were trimmed and assembled sepa- two populations of C. concholepas (1000 km apart) based on data rately, and combined using CLC Genomics Workbench software (CLC from RNA-seq. Differences in gene expression were confirmed with bio, Denmark). The overlap settings used for these assemblies were real time qPCR analysis. mismatch cost=2, insert cost=3, minimum contig length=200 bp, similarity=0.8, and trimming quality score=0.05. The redundancy of 2. Material and methods singleton sequences was calculated using the Java application Duplicate Finder (http://bioinfotutlets.blogspot.com/). All contigs and singletons 2.1. Samples were annotated according to Gene Ontology terms using Blast2Go soft- ware (Conesa et al., 2005). From the annotated contigs, we selected Fifty adult individuals of C. concholepas (Gastropoda, Muricidae) were those that presented homology with genes present in the mitochondrial collected as samples from two locations: Caleta Chungungo, Punta de genome, and we assembled them using the mitochondrial genome of Choros (29°16′S–71°31′W) and Los Molinos, Valdivia (39°40′S–73°12′ the muricid Thais clavigera (GenBank accession no. DQ159954.1) as a ref- W) in February 2010. Shell length of samples were between 10.2 and erence (Ki et al., 2010). As well, for regions of the mitochondrial genome 12.7 cm. Approximately 100 mg of gill and muscle tissue was extracted that were not covered by EST, specific primers were designed and ampli- from the samples and conserved in 1 mL of RNAlater stabilization solu- fied by PCR and Sanger sequencing (Macrogen Inc., S. Korea) of the tion (Ambion®, USA) and stored at −80 °C until total RNA extraction. amplicons (Table
Load more

Recommended publications
  • Concholepas Concholepas
    ANNALS OF THE SOUTH AFRICAN MUSEUM ANNALE VAN DIE SUID-AFRIKAANSE MUSEUM Volume 97 Band October 1985 Oktober Part 1 Deel THE FOSSIL OCCURRENCE IN SOUTHERN AFRICA OF THE SOUTH AMERICAN INTERTIDAL MOLLUSC CONCHOLEPAS CONCHOLEPAS By BRIAN KENSLEY are issued in parts at irregular intervals as material becomes available word uitgegee in dele op ongereelde tye na gelang van die beskikbaarheid van stof 1,2(1-3,5-8),3(1-2,4-5,8, I.-p.i.), 5(1-3, 5, 7-9), 6(1, I.-p.i.), 7(1-4), 8, 9(1-2, 7), 10(1-3), 11(1-2,5,7, I.-p.i.), 14(1-2), 15(4-5),24(2),27,31(1-3),32(5),33,36(2),45(1) Printed in South Africa by In Suid-Afrika gedruk deur The Rustica Press, Pty., Ltd., Die Rustica-pers, Edms., Bpk., Court Road, Wynberg, Cape Courtweg, Wynberg, Kaap THE FOSSIL OCCURRENCE IN SOUTHERN AFRICA OF THE SOUTH AMERICAN INTERTIDAL MOLLUSC CONCHOLEPASCONCHOLEPAS BRIAN KENSLEY National Museum of Natural History, Smithsonian Institution, Washington, D. C. The occurrence of the thaidid gastropod genus Concholepas is recorded from presumed Late Pleistocene coastal deposits in southern South West Africa-Namibia. The material is indistinguishable from C. concholepas, a species known from the Pliocene to Recent on the west coast of South America. The living species characteristically occurs in cold-temperate waters from the intertidal to depths of 40 m. It is suggested that the southern African fossils represent a short-lived pioneer population, established by larvae drifting from South America.
    [Show full text]
  • (Bruguiere, 1789) (Gastropoda, Muricidae) in Fjords and Channels of Southern Chile Revista Chilena De Historia Natural, Vol
    Revista Chilena de Historia Natural ISSN: 0716-078X [email protected] Sociedad de Biología de Chile Chile MOLINET, CARLOS; ARÉVALO, ALEJANDRA; GONZÁLEZ, MARÍA TERESA; MORENO, CARLOS A.; ARATA, JAVIER; NIKLITSCHEK, EDWIN Patterns of larval distribution and settlement of Concholepas concholepas (Bruguiere, 1789) (Gastropoda, Muricidae) in fjords and channels of southern Chile Revista Chilena de Historia Natural, vol. 78, núm. 3, 2005, pp. 409-423 Sociedad de Biología de Chile Santiago, Chile Available in: http://www.redalyc.org/articulo.oa?id=369944275005 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative LARVAL ECOLOGY OF C. CONCHOLEPAS IN SOUTHERNRevista CHILE Chilena de Historia Natural409 78: 409-423, 2005 Patterns of larval distribution and settlement of Concholepas concholepas (Bruguiere, 1789) (Gastropoda, Muricidae) in fjords and channels of southern Chile Patrones de distribución de larvas y asentamiento de Concholepas concholepas (Bruguiere, 1789) (Gastropoda, Muricidae) en fiordos y canales del sur de Chile CARLOS MOLINET1,3, ALEJANDRA ARÉVALO1, MARÍA TERESA GONZÁLEZ2, CARLOS A. MORENO2, JAVIER ARATA2 & EDWIN NIKLITSCHEK1 1Centro Universitario de la Trapananda e 2Instituto de Ecología y Evolución, 3Instituto de Acuicultura, Universidad Austral de Chile, Casilla 567, Valdivia, Chile; e-mail: [email protected] ABSTRACT The distribution of Concholepas concholepas (Mollusca, Gastropoda, Muricidae) is limited to the coasts of Chile and southern Peru. Almost all studies of this gastropod have been carried out in open coastal systems, rather than the fjords and channels of southern Chile, despite the fact that this area represents ca.
    [Show full text]
  • Hemocyanin of the Molluscan Concholepas Concholepas Exhibits an Unusual Heterodecameric Array of Subunits*
    THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 279, No. 25, Issue of June 18, pp. 26134–26142, 2004 © 2004 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in U.S.A. Hemocyanin of the Molluscan Concholepas concholepas Exhibits an Unusual Heterodecameric Array of Subunits* Received for publication, January 28, 2004, and in revised form, April 7, 2004 Published, JBC Papers in Press, April 8, 2004, DOI 10.1074/jbc.M400903200 Pablo De Ioannes‡, Bruno Moltedo‡, Harold Oliva, Rodrigo Pacheco, Fernando Faunes, Alfredo E. De Ioannes, and Marı´a Ine´s Becker§ From the Department of Research and Development, BIOSONDA Corp., Avenida Alcalde Eduardo Castillo Velasco 2902, Santiago 7750269, Chile We describe here the structure of the hemocyanin Molluscan hemocyanins assemble into hollow cylindrical lay- from the Chilean gastropod Concholepas concholepas ered molecules formed by 10 subunits. Each subunit, ranging (CCH), emphasizing some attributes that make it inter- from 350 to 450 kDa, includes seven or eight globular folded esting among molluscan hemocyanins. CCH exhibits a domains known as functional units (FUs),1 which are arranged predominant didecameric structure as revealed by elec- into pearl-like chains and covalently bound by a short flexible tron microscopy and a size of 8 MDa by gel filtration, linker region of 10–15 amino acid residues. FUs vary in size and, in contrast with other mollusc hemocyanins, its from 45 to 55 kDa, and each of them is capable of reversibly 2؉ stabilization does not require additional Ca and/or binding one oxygen molecule through a pair of copper atoms. Downloaded from 2؉ Mg in the medium.
    [Show full text]
  • Shelled Molluscs
    Encyclopedia of Life Support Systems (EOLSS) Archimer http://www.ifremer.fr/docelec/ ©UNESCO-EOLSS Archive Institutionnelle de l’Ifremer Shelled Molluscs Berthou P.1, Poutiers J.M.2, Goulletquer P.1, Dao J.C.1 1 : Institut Français de Recherche pour l'Exploitation de la Mer, Plouzané, France 2 : Muséum National d’Histoire Naturelle, Paris, France Abstract: Shelled molluscs are comprised of bivalves and gastropods. They are settled mainly on the continental shelf as benthic and sedentary animals due to their heavy protective shell. They can stand a wide range of environmental conditions. They are found in the whole trophic chain and are particle feeders, herbivorous, carnivorous, and predators. Exploited mollusc species are numerous. The main groups of gastropods are the whelks, conchs, abalones, tops, and turbans; and those of bivalve species are oysters, mussels, scallops, and clams. They are mainly used for food, but also for ornamental purposes, in shellcraft industries and jewelery. Consumed species are produced by fisheries and aquaculture, the latter representing 75% of the total 11.4 millions metric tons landed worldwide in 1996. Aquaculture, which mainly concerns bivalves (oysters, scallops, and mussels) relies on the simple techniques of producing juveniles, natural spat collection, and hatchery, and the fact that many species are planktivores. Keywords: bivalves, gastropods, fisheries, aquaculture, biology, fishing gears, management To cite this chapter Berthou P., Poutiers J.M., Goulletquer P., Dao J.C., SHELLED MOLLUSCS, in FISHERIES AND AQUACULTURE, from Encyclopedia of Life Support Systems (EOLSS), Developed under the Auspices of the UNESCO, Eolss Publishers, Oxford ,UK, [http://www.eolss.net] 1 1.
    [Show full text]
  • Introduction to Marine Ecology and Conservation
    New York University Version: 2/19/21 ENVST-UA/ANST-UA 323 Marine Ecology & Conservation Spring 2021, T/R 3:30-4:45pm (4 credits) Prerequisite: ENVST-UA 100 Env. Systems Science Location: Zoom Professor: Jennifer Jacquet [email protected] Office hours: T/R 4:45-5:45 or by appointment; just drop an email Office location: Zoom Course Assistant: The Course Objectives: Welcome! This course analyzes several aspects of our oceans, with particular emphasis on human impacts and examines some of the recent major threats, including lack of governance for the high seas, deep-sea mining, and the effects of climate change on aquatic animals. We will focus ecological relationships between marine organisms and their environment, with the introduction of humans as marine predators and ecological disturbers. We will review recent peer-reviewed marine ecology studies as well as popular articles to familiarize ourselves with the latest research. The first half of the course focuses more on basic ecology while the second half focuses more on anthropogenic impacts (e.g., overexploitation, pollution, invasive species, and climate change) and proposed and tested solutions. Note: To try to capture the ‘present’ nature of the classroom, I ask that all students attend class in real time unless you have an exception (due to time zone, etc.) and, whenever possible, please have your camera on. This is also to honor the guests who will join us and who are happier when speaking to interested faces, as opposed to black boxes. Required texts: The peer-reviewed research articles listed on the daily calendar (all available on NYU Classes) and one film and one book (which you will likely need to purchase).
    [Show full text]
  • Concholepas Concholepas (Loco)
    Ocean Acidification Disrupts Prey Responses to Predator Cues but Not Net Prey Shell Growth in Concholepas concholepas (loco) Patricio H. Manrı´quez1*,Marı´a Elisa Jara1,Marı´a Loreto Mardones1, Jorge M. Navarro1, Rodrigo Torres2, Marcos A. Lardies3, Cristian A. Vargas4, Cristian Duarte5, Stephen Widdicombe6, Joseph Salisbury7, Nelson A. Lagos8 1 Instituto de Ciencias Marinas y Limnolo´gicas, Laboratorio de Ecologı´a y Conducta de la Ontogenia Temprana (LECOT) and Laboratorio Costero de Recursos Acua´ticos de Calfuco. Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile, 2 Centro de Investigacio´n en Ecosistemas de la Patagonia (CIEP), Coyhaique, Chile, 3 Facultad de Artes Liberales, Universidad Adolfo Iba´n˜ez, Santiago, Chile, 4 Laboratorio de Funcionamiento de Ecosistemas Acua´ticos (LAFE), Unidad de Sistemas Acua´ticos, Centro de Ciencias Ambientales EULA, Universidad de Concepcio´n, Concepcio´n, Chile, 5 Facultad de Ecologı´a y Recursos Naturales, Departamento de Ecologı´a y Biodiversidad, Universidad Andre´s Bello, Santiago Chile, 6 Plymouth Marine Laboratory, Prospect Place, West Hoe, Plymouth, Devon, United Kingdom, 7 Ocean Processes Analysis Lab, University of New Hampshire, Durham, New Hampshire, United States of America, 8 Facultad de Ciencias, Universidad Santo Toma´s, Ejercito, Santiago, Chile Abstract Background: Most research on Ocean Acidification (OA) has largely focused on the process of calcification and the physiological trade-offs employed by calcifying organisms to support the building of calcium carbonate structures. However, there is growing evidence that OA can also impact upon other key biological processes such as survival, growth and behaviour. On wave-swept rocky shores the ability of gastropods to self-right after dislodgement, and rapidly return to normal orientation, reduces the risk of predation.
    [Show full text]
  • Gayana 73(1): 17-27, 2009 ISSN 0717-652X
    Gayana 73(1): 17-27, 2009 ISSN 0717-652X MOLECULAR ANALYSIS IN CHILEAN COMMERCIAL GASTROPODS BASED ON 16S rRNA, COI AND ITS1-5.8S rDNA-ITS2 SEQUENCES ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2 Felipe Aguilera-Muñoz, Fabiola Lafarga-Cruz & Cristian Gallardo-Escárate* Laboratorio de Biotecnología Acuícola, Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Centro de Biotecnología, Universidad de Concepción. Barrio Universitario s/n. Casilla 160-C Concepción, Chile. email: [email protected] ABSTRACT Gastropod mollusks are part of the principal marine resources cultivated and commercialized in Chile. There are native Chilean species such as loco (Concholepas concholepas), locate (Thais chocolata), trumulco snail (Chorus giganteus), keyhole limpets (Fissurella spp.), tegula snail (Tegula atra) as well as exotic species such as red abalone (Haliotis rufescens) and Japanese abalone (Haliotis discus hannai). Despite their importance as marine resources, molecular genetic studies establishing phylogenetic relationships and estimating population genetic parameters are scarce. The aim of this study is to establish a molecular approach among the main commercial gastropod species in Chile. The mitochondrial genes 16S rRNA and COI, and the nuclear ribosomal region ITS1-5.8SrDNA-ITS2 were amplified by PCR and sequencing. Alignment analysis was used to determine systematic relationships at the specific level for the species studied. The results revealed that 7 species are grouped in 4 genetically distinct families (Haliotidae, Trochidae, Muricidae and Fissurellidae). In comparison with COI sequencing, 16S rRNA and ITS1-5.8SrDNA-ITS2 sequencing were relatively more conserved with a divergence percentage for 16S rDNA and ITS1-5.8SrDNA-ITS2 of 1.2% and 1.8%, respectively, contrasting with the value of 10% obtained for COI in abalone.
    [Show full text]
  • Cenozoic Fossil Mollusks from Western Pacific Islands; Gastropods (Eratoidae Through Harpidae)
    Cenozoic Fossil Mollusks From Western Pacific Islands; Gastropods (Eratoidae Through Harpidae) GEOLOGICAL SURVEY PROFESSIONAL PAPER 533 Cenozoic Fossil Mollusks From Western Pacific Islands; Gastropods (Eratoidae Through Harpidae) By HARRY S. LADD GEOLOGICAL SURVEY PROFESSIONAL PAPER 533 Descriptions or citations of 195 representatives of 21 gastropod families from 7 island groups UNITED STATES GOVERNMENT PRINTING OFFICE, WASHINGTON : 1977 UNITED STATES DEPARTMENT OF THE INTERIOR CECIL D. ANDRUS, Secretary GEOLOGICAL SURVEY V. E. McKelvey, Director Library of Congress Cataloging in Publication Data Ladd, Harry Stephen, 1899- Cenozoic fossil mollusks from western Pacific islands. (Geological Survey professional paper ; 533) Bibliography: p. Supt. of Docs, no.: I 19.16:533 1. Gastropoda, Fossil. 2. Paleontology Cenozoic. 3. Paleontology Islands of the Pacific. I. Title. II. Series: United States. Geological Survey. Professional paper ; 533. QE75.P9 no. 533 [QE808] 557.3'08s [564'.3'091646] 75-619274 For sale by the Superintendent of Documents, U.S. Government Printing Office Washington, D.C. 20402 Stock Number 024-001-02975-8 CONTENTS Page Page Abstract _ _ _ _ ___ _ _ 1 Paleontology Continued Introduction ____ _ _ __ 1 Systematic paleontology Continued 1 Families covered in the present paper Continued Stratigraphy and correlation _ _ _ q Cymatiidae 33 6 35 Tonnidae __ _______ 36 6 Ficidae _ - _ _ _ _ ___ 37 Fiji _ __ __ __ _____ __ ____ __ _ 6 37 New Hebrides 7 Thaididae __ _ _ _ _ _ 39 14 41 14 Columbellidae - 44 14 Buccinidae _ - - 49 51 (1966, 1972) 14 Nassariidae _ - 51 "P1 ?} TYllllPQ.
    [Show full text]
  • Marine Ecology Progress Series 229:95
    MARINE ECOLOGY PROGRESS SERIES Vol. 229: 95–104, 2002 Published March 20 Mar Ecol Prog Ser Temporal and spatial variation in the distribution of epineustonic competent larvae of Concholepas concholepas along the central coast of Chile Elie Poulin, Alvaro T. Palma*, Germán Leiva, Eduardo Hernández, Pilar Martínez, Sergio A. Navarrete**, Juan C. Castilla Departamento de Ecología, Estación Costera de Investigaciones Marinas Las Cruces and Center for Advanced Studies in Ecology and Biodiversity, Pontificia Universidad Católica de Chile, Alameda 340, Casilla 114-D, Santiago CP 6513677, Chile ABSTRACT: The abundance of competent epineustonic larvae of the gastropod Concholepas concholepas (Gastropoda: Muricidae) in nearshore waters at 2 sites along the central coast of Chile was examined through monthly plankton tows from July 1999 to June 2000. Larvae were found in plankton collections from July 1999 to February 2000 with maximum abundance in September and October. Settlement in artificial collectors deployed onshore on the lower intertidal zone showed the same unimodal pattern with a settlement peak during October and November. Variation in larval distribution among sampling dates was related to the occurrence of north-south winds. We found that C. concholepas larvae were more abundant closer to shore after moderate southerly wind periods than on calm days, probably because of the shoreward advection of the upper sea surface layer. While sampling during a strong coastal upwelling event (produced by strong southwesterly winds), C. concholepas larvae were only found in the upwelled waters between the front and the coast. This unusual pattern contrasts with what would be expected for typical epineustonic larvae, suggesting the existence of a mechanism of transport or retention by which C.
    [Show full text]
  • Redalyc.Paleobiogeography and Taxonomy of the Genus Concholepas
    Revista Chilena de Historia Natural ISSN: 0716-078X [email protected] Sociedad de Biología de Chile Chile CÁRDENAS, LEYLA; VIARD, FRÉDÉRIQUE; CASTILLA, JUAN CARLOS Paleobiogeography and taxonomy of the genus Concholepas (Lamarck, 1801): a review and new evidences Revista Chilena de Historia Natural, vol. 81, núm. 3, 2008, pp. 417-436 Sociedad de Biología de Chile Santiago, Chile Available in: http://www.redalyc.org/articulo.oa?id=369944287010 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative PALEOBIOGEOGRAPHY AND TAXONOMY GENUS CONCHOLEPASRevista Chilena de Historia Natural417 81: 417-436, 2008 Paleobiogeography and taxonomy of the genus Concholepas (Lamarck, 1801): a review and new evidences Paleobiogeografía y taxonomía del género Concholepas (Lamarck, 1801): una revisión y nuevas evidencias LEYLA CÁRDENAS1, 3, *, FRÉDÉRIQUE VIARD2 & JUAN CARLOS CASTILLA1 1 Center for Advanced Studies in Ecology and Biodiversity (CASEB), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Chile 2 Equipe Evolution et Génétique des Populations Marines, LIA “DIAMS”, UMR 7144 CNRS-Université Pierre et Marie Curie, Station Biologique Roscoff, France 3 Instituto de Ecología y Evolución, Universidad Austral de Chile, Campus Isla Teja, Casilla 567, Valdivia, Chile *e-mail for correspondence: [email protected] ABSTRACT The muricid gastropod Concholepas concholepas, known in Chile as ‘loco’, is an important component of intertidal and shallow subtidal communities, and is one of the main invertebrates targeted by small-scale fishers (divers) in Chile. Because of its ecological importance and economical value, numerous studies have been conducted to describe its life history, ecology and to understand population dynamics, fishery and management.
    [Show full text]
  • Significance of Marine Protected Areas in Central Chile As Seeding Grounds for the Gastropod Concholepas Concholepas
    MARINE ECOLOGY PROGRESS SERIES Vol. 215: 201–211, 2001 Published May 31 Mar Ecol Prog Ser Significance of marine protected areas in central Chile as seeding grounds for the gastropod Concholepas concholepas Patricio H. Manríquez, Juan Carlos Castilla* Estación Costera de Investigaciones Marinas and Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile ABSTRACT: The importance of non-harvested areas, marine protected areas, and management and exploitation areas (= harvest-controlled) as seeding areas of Concholepas concholepas (Bruguière, 1789) larvae was quantitatively evaluated at intertidal and subtidal sites at Las Cruces, central Chile. Egg capsules of C. concholepas were sampled along both intertidal and subtidal strip-transects monthly in harvested, harvest-controlled and non-harvested areas from September 1990 to De- cember 1993. Additionally, egg capsules of C. concholepas were sampled at subtidal sites in the 3 categories of areas during 1993 and 1994. Spawning activity of C. concholepas was consistently con- centrated between February and July in both the intertidal and the subtidal zones. The total area occupied by egg capsules of C. concholepas was larger in non-harvested than in harvested areas; moreover, larger capsules were found in non-harvested areas. These differences in total surface occupied by egg capsules and their sizes have an important impact on the estimated number of C. concholepas larvae that would be released from harvested and non-harvested areas. We conclude that protected areas may play an important role in the natural replenishment of C. concholepas stocks. KEY WORDS: Marine protected areas · Harvested areas · Seeding grounds · Concholepas concholepas · Egg capsule · Larvae · Intertidal · Subtidal · Chile Resale or republication not permitted without written consent of the publisher INTRODUCTION harvesting systems (e.g.
    [Show full text]
  • Oceanography and Marine Biology an Annual Review Volume 55
    Oceanography and Marine Biology An Annual Review Volume 55 S.J. Hawkins, A.J. Evans, A.C. Dale, L.B. Firth, D.J. Hughes, and I.P. Smith First Published 2017 ISBN 978-1-138-19786-2 (hbk) ISBN 978-1-351-98759-2 (ebk) Chapter 3 Ecological Dominance along Rocky Shores, with a Focus on Intertidal Ascidians Marc Rius, Peter R. Teske, Patricio H. Manríquez, Rocío Suárez-Jiménez, Christopher D. McQuaid, and Juan Carlos Castilla (CC BY-NC-ND 4.0) Oceanography and Marine Biology: An Annual Review, 2017, 55, 2-33 © S. J. Hawkins, D. J. Hughes, I. P. Smith, A. C. Dale, L. B. Firth, and A. J. Evans, Editors Taylor & Francis ECOLOGICAL DOMINANCE ALONG ROCKY SHORES, WITH A FOCUS ON INTERTIDAL ASCIDIANS MARC RIUS1,2*, PETER R. TESKE2, PATRICIO H. MANRÍQUEZ3, ROCÍO SUÁREZ-JIMÉNEZ4, CHRISTOPHER D. MCQUAID5 & JUAN CARLOS CASTILLA6 1Ocean and Earth Science, University of Southampton, National Oceanography Centre, European Way, Southampton, SO14 3ZH, United Kingdom 2Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, Auckland Park, 2006, South Africa 3Laboratorio de Ecología y Conducta de la Ontogenia Temprana (LECOT), Centro de Estudios Avanzados en Zonas Áridas (CEAZA), Coquimbo, Avenida Ossandón 877, Coquimbo, Chile 4Department of Botany, University of Otago, Dunedin 9054, New Zealand 5Coastal Research Group, Department of Zoology and Entomology, Rhodes University, Grahamstown, South Africa 6Centro de Conservación Marina, Estación Costera de Investigaciones Marinas (ECIM), Núcleo de Conservación Marina. Iniciativa Científica Milenio. Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile *Corresponding author: Marc Rius e-mail: [email protected] The role of dominant species is of central importance in ecology.
    [Show full text]