Structural Analysis of the Legionella Pneumophila Dot/Icm Type IV Secretion System Core Complex
bioRxiv preprint doi: https://doi.org/10.1101/2020.08.28.271460; this version posted August 28, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Structural analysis of the Legionella pneumophila Dot/Icm Type IV Secretion System 2 Core Complex 3 4 Clarissa L. Durie1†, Michael J. Sheedlo2†, Jeong Min Chung1, Brenda G. Byrne3, Min Su1, 5 Thomas Knight3, Michele S. Swanson3*, D. Borden Lacy2,4*, and Melanie D. Ohi1,5* 6 †These authors contributed equally to this work 7 *To whom correspondence should be addressed 8 9 Author Affiliations 10 Life Sciences Institute, University of Michigan, Ann Arbor MI, USA1 11 Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 12 Nashville TN, USA2 13 Department of Microbiology and Immunology, University of Michigan, Ann Arbor MI, USA3 14 The Veterans Affairs Tennessee Valley Healthcare System, Nashville TN, USA4 15 Department of Cell and Developmental Biology, University of Michigan, Ann Arbor MI, USA5 16 17 Abstract 18 Legionella pneumophila is an opportunistic pathogen that causes the potentially fatal 19 pneumonia Legionnaires’ Disease. This infection and subsequent pathology require the Dot/Icm 20 Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to 21 prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components 22 including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, 23 and DotH.
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