Successful Recovery of Nuclear Protein-Coding Genes from Small Insects in Museums Using Illumina Sequencing Kanda, K., Pflug, J. M., Sproul, J. S., Dasenko, M. A., & Maddison, D. R. (2015). Successful Recovery of Nuclear Protein-Coding Genes from Small Insects in Museums Using Illumina Sequencing. PLoS ONE, 10(12), e0143929. doi:10.1371/journal.pone.0143929 10.1371/journal.pone.0143929 Public Library of Science Version of Record http://cdss.library.oregonstate.edu/sa-termsofuse RESEARCH ARTICLE Successful Recovery of Nuclear Protein- Coding Genes from Small Insects in Museums Using Illumina Sequencing Kojun Kanda1☯*, James M. Pflug1☯, John S. Sproul1☯, Mark A. Dasenko2, David R. Maddison1☯ 1 Department of Integrative Biology, Oregon State University, Corvallis, Oregon, United States of America, 2 Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon, United States of America a11111 ☯ These authors contributed equally to this work. *
[email protected] Abstract OPEN ACCESS In this paper we explore high-throughput Illumina sequencing of nuclear protein-coding, ribosomal, and mitochondrial genes in small, dried insects stored in natural history collec- Citation: Kanda K, Pflug JM, Sproul JS, Dasenko MA, Maddison DR (2015) Successful Recovery of tions. We sequenced one tenebrionid beetle and 12 carabid beetles ranging in size from 3.7 Nuclear Protein-Coding Genes from Small Insects in to 9.7 mm in length that have been stored in various museums for 4 to 84 years. Although Museums Using Illumina Sequencing. PLoS ONE we chose a number of old, small specimens for which we expected low sequence recovery, 10(12): e0143929. doi:10.1371/journal.pone.0143929 we successfully recovered at least some low-copy nuclear protein-coding genes from all Editor: Patrick O'Grady, University of California, specimens.