Product Specifications analyses n high acquisitionhigh rates Orbitrap in and linear ion trap analyzers and maximum exibility for dissociation and detection fl of fragment ions dischargeTownsend with extremely stable ux forimproved usability and anion fl longevity reagent Orbitrap and linear ion trap MS peptide identifi cations without method peptide identifi optimization for samples of unknown concentration, reducing sample and instrument time requirements for routine experiments cation identifi peptide es method development interface simplifi ows and enables unique and complex workfl Unique Tribrid architectureUnique Tribrid allows for • Compact ETD ion source based on • Acquisition rates of up to 20 Hz for both • • Universal Method provides maximal • exible drag-and-drop user Intuitive and fl • analyses analyses n (All Dynamically ™ with detection either in n experiments n 200 the Orbitrap or linear ion trap detector HCD and optional ETD and EThcD available at any stage of MS with intelligent ADAPT mass fi lter, linear ion trap andlter, Orbitrap mass fi massanalyzers ultra-high resolution and the fastest rates acquisition Orbitrap delity240,000 up to FWHMwith isotopic fi at m/z cantlyimproved robustness and signifi life span Available Parallelizable technologyAvailable Time) MS and MS • Multiple fragmentation techniques: CID, • Established Features Tribrid architectureTribrid includes quadrupole • eld Orbitrap analyzer for Ultra high-fi • Resolving power up to 500,000 FWHM,• Large surface area ion trap detector for • Full parallelization of MS and MS • • Synchronous Precursor Selection (SPS)• for

™ Breakthrough Gains for Quantitative Biology Quantitative for Gains Breakthrough Sensitivity Transformed Lumos ™ combines a High Capacity Transfer and anTube Electrodynamic Ion Funnel ux and lower limits for increased ion fl detectionof prevents neutrals and high velocity clusters from entering the resolving quadrupole (AQT) combines high selectivity and ciencyeffi of transfer for the selected acrosssymmetrically ions the isolation window for improved transmission of high molecular weight ions to the Orbitrap analyzer cantly increasedETD providing signifi fragment ion coverage Orbitrap Fusion Novel Orbitrap Fusion Lumos MS Features Novel high-sensitivity API interface• Advanced Active Beam Guide (AABG)• Advanced Quadrupole Technology • Advanced Vacuum Technology • Novel ETD HD—high dynamic range•

Thermo Scientifi c Scientifi Thermo Orbitrap Lumos Fusion Spectrometer Mass Tribrid ™ MASS SPECTROMETRY mass spectrometer is the industry-leading ™ The Thermo Scientifi c The Thermo Scientifi Technology, Advanced Vacuum Technology, and ETD HD. Technology, Advanced Vacuum Technology, Tube, ElectrodynamicTube, Ion Funnel, Advanced Quadrupole sensitivity facilitated by a new High Capacity Transfer Capacity Transfer High new a by sensitivity facilitated high-performance mass spectrometer enhanced with Tribrid 2

Hardware Specifications • High-speed real-time data acquisition Performance Characteristics and instrument control system Ion Source Mass Range • Automatic calibration of all ion transfer Thermo Scientific™ Ion Max NG™ and analysis parameters via instrument m/z 50–2,000 Ion Source control software m/z 200–4,000 • Adjustable heated electrospray ionization up to m/z 6,000 without precursor ion (HESI) probe for ultimate sensitivity Dual-Pressure Linear Ion Trap selection (OTMS only) n • Flow rates from 1 μL/min up to • High-pressure cell for MS precursor 2,000 μL/min ion isolation from 0.2 u to 600 u Resolution OTMS • Designed for maximum performance • Collision-induced dissociation (CID) and 15,000–500,000 (FWHM) at m/z 200 with no need for adjustments optional electron-transfer dissociation (ETD) Scan Rate* • Enhanced ruggedness • Low-pressure cell for improved scan OTMSn up to 20 Hz ™ • Sweep Gas reduces chemical noise speed, resolving power, and mass ITMSn up to 20 Hz • Optional APCI probe compatible with liquid accuracy Mass Accuracy OTMS* flow rates of 50 μL/min to 2,000 μL/min • Dual-dynode detector with high linear without splitting dynamic range for improved quantitation < 3 ppm RMS using external calibration and a large surface area for increased < 1 ppm RMS using internal calibration Generation IV Ion Optics lifespan and robustness The new API interface consists of a High MS/MS Electrospray Ionization (ESI) Ion Trap sensitivity: Capacity Transfer Tube (HCTT) and an Vacuum System Electrodynamic Ion Funnel (EDIF) • Split-flow turbomolecular pump controlling 2 μL of a 50 fg/μL solution of reserpine vacuum in three regions (100 femtograms total) injected at a flow High-Capacity Ion Transfer Tube • Aluminum high-vacuum analyzer chambers of 500 μL/min will produce a minimum • The HCTT increases ion flux into the • Advanced Vacuum Technology reducing signal-to-noise ratio of 200:1 for the vacuum system for improved sensitivity the pressure in the ultra-high vacuum transition of the isolated protonated and desolvation 10 region to <2 × 10 and enhancing molecular ions at m/z 609 to the largest two • Vent-free maintenance transmission of the ions to the Orbitrap mass analyzer product ions, m/z 397 and m/z 448, when Electrodynamic Ion Funnel the mass spectrometer is operated at unit Options • EDIF, a radio frequency (RF) device, resolution in the full scan MS/MS mode, m/z efficiently captures ions as they leave Thermo Scientific™ NanoSpray Flex NG™ 165–615. The test requires HESI III probe. the HCTT Ion Source Dynamic Range • Automatic tuning results in broad • Supports static and dynamic > 5,000 within a single OTMS spectrum transmission curves and reduces ion nanoelectrospray experiments losses, increasing sensitivity • Compatible with liquid flow rates of MS Scan Power 50 nL/min to 2 μL/min Advanced Active Beam Guide MSn, for n = 1 through 10

• Advanced Active Beam Guide with an axial ™ ™ Thermo Scientific EASY-Spray Synchronous Precursor Isolation field and low pass filtering reduces noise Ion Source by preventing neutrals and high-velocity Up to 20 precursors per MSn scan clusters from entering the quadrupole • Maximum nanoelectrospray performance mass filter with no need for adjustments Multiplexing Using the Ion Routing Multipole Thermo Scientific™ EASY-ETD Ion Source Advanced Quadrupole Technology Up to 10 precursors per scan using the • Segmented Quadrupole Mass Filter for • Improved dynamic range and S/N for ™ quadrupole mass filter precursor ion selection with variable ETD fragments using Easy ETD HD precursor isolation width from 0.4 u • Generates fluoranthene anions for electron Polarity Switching to 1,200 u transfer dissociation (ETD) One full cycle in < 1.1 sec (one full scan • Superior ion transmission across isolation • Townsend discharge provides extremely positive mode and one full scan negative widths up to 1,200 u stable and robust ionization mode at resolution setting of 30,000) • MS/MS precursor ion selection with high • Compact size, located entirely within the transmission from 50 to 3,000 m/z footprint of the instrument ETD Efficiency • Active reagent ion filtering using the ETD fragmentation efficiency of >15%, Ion-Routing Multipole quadrupole mass filter (infusion of 1 pmol/μL angiotensin at 3 μL/min) • IRM for ion storage • The ion sorting routines and charge state • Higher-energy collisional dissociation dependent calibration of the ETD reaction Analog Inputs (HCD) cell time maximize ETD spectral quality Channel 1 analog input (0–10 V), • Efficient, reliable ion transfer between the ™ ™ Channel 2 analog (0–2 V) ion-routing multipole and the Orbitrap and Thermo Scientific EASY-IC Ion Source linear ion trap mass analyzers • Generates internal calibrant ions for *Under defined conditions real-time mass calibration of MS scans Orbitrap Mass Analyzer in both positive and negative modes • Next-generation, ultra high-field Orbitrap • Provides <1 ppm RMS mass analyzer accuracy under defined conditions • New low noise detection pre-amplifier and minimizes effort 3

Software Features Exclusive Technologies • Synchronous MS3 precursor selection • Dynamic Scan Management allows significantly increases the number of Data System peptides accurately quantified in isobaric ® for intelligent, real-time scheduling, • High-performance PC with Intel parallelization, and prioritization of the mass tagging experiments microprocessor scan events. It also enables selection, • Data-dependent neutral-loss experiment • High-resolution LCD color monitor sorting, and routing of precursors to triggers an MS3 scans only for product ions • Microsoft® Windows® 7 operating system different fragmentation modes and with a predefined neutral loss analyzers based on user-selected • Microsoft Office 2010 software • Isolation offset allows for custom centering parameters, including precursor m/z, of the window when working with broad • Thermo Scientific™ Xcalibur™ processing intensity, and/or charge isotopic distributions and instrument control software • Precursor fragmentation can take place • Quanfirmation experiment uses • Tune editor for system calibration, in the ion-routing multipole (HCD), in the synchronous HRAM SIM for quantitation diagnostics, and manual data acquisition ion trap (CID, ETD) or both (EThcD) with with full-scan MS/MS in the linear ion trap • Method editor with comprehensive fragment detection in the linear ion trap or for precursor identity confirmation application-specific template library and Orbitrap mass analyzers at any stage of drag-and-drop user interface to facilitate MSn analysis Optional Application-Specific Software method development • All Dynamically Available Parallelizable • Thermo Scientific™ Proteome Discoverer™ Time (ADAPT™) technology determines in software—flexible, expandable platform Operation Modes real time the most efficient parallelization for the analysis of qualitative and • Top-speed mode maximizes the number of quadrupole mass filter, Orbitrap and quantitative proteomics data of high-quality MSn spectra from each linear ion trap operations • Thermo Scientific™ Protein Deconvolution cycle by intelligently scheduling MS and • Automatic gain control (AGC) ensures that software—intact-protein analysis platform data-dependent MSn scans based on a the ion routing multipole is always filled • Thermo Scientific™ ProSightPC™ user-definable time between adjacent with the optimum number of ions for any software—stand-alone software for survey scans acquisition type analyzing top-down intact-protein data, as • Dynamic parallelization using ADAPT™ well as middle-down and bottom-up data technology maximizes the amount of Advanced Data-Dependent Experiments ™ ™ high-quality data acquired by synchronizing • Universal Method powered by ADAPT • Thermo Scientific Compound Discoverer operation of the quadrupole mass filter, technology always ensures the best software—integrated solution for ion-routing multipole, linear ion trap and results from the samples with unknown small-molecule structural identification Orbitrap mass analyzers concentrations • Thermo Scientific™ SIEVE™ software— • Fragment ion or neutral-loss-triggered label-free, semi-quantitative differential MSn experiments including at the same analysis of highly complex data sets MSn level

Ultra-High Field Orbitrap Dual-Pressure Mass Analyzer Linear Ion Trap

Low Pressure Cell Advanced Advanced Quadrupole High Pressure Actve Beam Technology Cell Guide

Ion Routing Multipole

EASY-ETD/IC C-Trap Ion Source Electrodynamic Ion Funnel

High Capacity Transfer Tube

Figure 1. Orbitrap Fusion Lumos MS ion path. Product Specifications Product Installation Requirements Size IC/ETD Options Power 1186 × 674 × 650 mm (w, d, h) Nitrogen supply for IC/ETD option: Ultra-high purity nitrogen (UHP, 99.999%) with less • 230 Vac ± 10% single phase, 15 Amp, Weight 50/60 Hz, with earth ground for the than one ppm each water and oxygen instrument 180 kg (400 pounds) without data system, vacuum rough pumps and optional items • 120 or 230 Vac single phase with earth ground for the data system Environment Gas • System averages 2,800 W (10,000 Btu/hr) output when considering air conditioning • Ultra-high purity Helium (99.999%) with needs less than 1 ppm each of water, oxygen and • Operating environment must be 16–26 °C total hydrocarbons (59–78 °F) and relative humidity must be • High purity Nitrogen (99.5% pure, flow 50–80% with no condensation rate 15 L/min) gas supply for the API • Optimum operating temperature is source and the ion routing multipole (IRM) 18–21 °C (65–70 °F)

1320

All dimensions in millimeters

788

1270 767

703

1441

130

1195 686

Figure 2. Orbitrap Fusion Lumos MS dimensions. www.thermoscientific.com/lumos ©2015 Thermo Fisher Scientific Inc. All rights reserved. ISO is a trademark of the International Standards Organization. Intel is a registered trademark of Intel Corporation. Microsoft and Windows are registered trademarks of Microsoft Corporation. All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries. This information is presented as an Thermo Fisher Scientific, example of the capabilities of Thermo Fisher Scientific products. It is not intended to encourage use of these products in any San Jose, CA USA is manners that might infringe the intellectual property rights of others. Specifications, terms and pricing are subject to change. ISO 13485 Certified. Not all products are available in all countries. Please consult your local sales representative for details. ISO 13485

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