MASS SPECTROMETRY Product Specifications Thermo Scientifi c Orbitrap Fusion Lumos Tribrid Mass Spectrometer Breakthrough Gains for Quantitative Biology Sensitivity Transformed The Thermo Scientifi c™ Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer is the industry-leading high-performance mass spectrometer with enhanced sensitivity facilitated by a new High Capacity Transfer Tube, Electrodynamic Ion Funnel, Advanced Quadrupole Technology, Advanced Vacuum Technology, and ETD HD. Novel Orbitrap Fusion Lumos MS Established Tribrid Features • Unique Tribrid architecture allows for Features • Tribrid architecture includes quadrupole high acquisition rates in Orbitrap and linear ion trap analyzers and maximum • Novel high-sensitivity API interface mass fi lter, linear ion trap and Orbitrap fl exibility for dissociation and detection combines a High Capacity Transfer mass analyzers of fragment ions Tube and an Electrodynamic Ion Funnel • Ultra high-fi eld Orbitrap analyzer for for increased ion fl ux and lower limits ultra-high resolution and the fastest • Compact ETD ion source based on of detection Orbitrap acquisition rates Townsend discharge with extremely stable anion fl ux for improved usability and • Advanced Active Beam Guide (AABG) • Resolving power up to 500,000 FWHM, reagent longevity prevents neutrals and high velocity with isotopic fi delity up to 240,000 FWHM clusters from entering the resolving at m/z 200 • Acquisition rates of up to 20 Hz for both Orbitrap and linear ion trap MSn analyses quadrupole • Large surface area ion trap detector for • Advanced Quadrupole Technology signifi cantly improved robustness and • Universal Method provides maximal (AQT) combines high selectivity and life span peptide identifi cations without method effi ciency of transfer for the selected n optimization for samples of unknown • Full parallelization of MS and MS analyses concentration, reducing sample and ions symmetrically across the with intelligent ADAPT™ (All Dynamically isolation window instrument time requirements for routine Available Parallelizable Time) technology peptide identifi cation experiments • Advanced Vacuum Technology • Synchronous Precursor Selection (SPS) for • Intuitive and fl exible drag-and-drop user for improved transmission of high MS and MSn experiments molecular weight ions to the interface simplifi es method development Orbitrap analyzer • Multiple fragmentation techniques: CID, and enables unique and complex workfl ows HCD and optional ETD and EThcD available • Novel ETD HD—high dynamic range at any stage of MSn with detection in either ETD providing signifi cantly increased the Orbitrap or linear ion trap detector fragment ion coverage 2 Hardware Specifications • High-speed real-time data acquisition Performance Characteristics and instrument control system Ion Source Mass Range • Automatic calibration of all ion transfer Thermo Scientific™ Ion Max NG™ and analysis parameters via instrument m/z 50–2,000 Ion Source control software m/z 200–4,000 • Adjustable heated electrospray ionization up to m/z 6,000 without precursor ion (HESI) probe for ultimate sensitivity Dual-Pressure Linear Ion Trap selection (OTMS only) n • Flow rates from 1 μL/min up to • High-pressure cell for MS precursor 2,000 μL/min ion isolation from 0.2 u to 600 u Resolution OTMS • Designed for maximum performance • Collision-induced dissociation (CID) and 15,000–500,000 (FWHM) at m/z 200 with no need for adjustments optional electron-transfer dissociation (ETD) Scan Rate* • Enhanced ruggedness • Low-pressure cell for improved scan OTMSn up to 20 Hz ™ • Sweep Gas reduces chemical noise speed, resolving power, and mass ITMSn up to 20 Hz • Optional APCI probe compatible with liquid accuracy Mass Accuracy OTMS* flow rates of 50 μL/min to 2,000 μL/min • Dual-dynode detector with high linear without splitting dynamic range for improved quantitation < 3 ppm RMS using external calibration and a large surface area for increased < 1 ppm RMS using internal calibration Generation IV Ion Optics lifespan and robustness The new API interface consists of a High MS/MS Electrospray Ionization (ESI) Ion Trap sensitivity: Capacity Transfer Tube (HCTT) and an Vacuum System Electrodynamic Ion Funnel (EDIF) • Split-flow turbomolecular pump controlling 2 μL of a 50 fg/μL solution of reserpine vacuum in three regions (100 femtograms total) injected at a flow High-Capacity Ion Transfer Tube • Aluminum high-vacuum analyzer chambers of 500 μL/min will produce a minimum • The HCTT increases ion flux into the • Advanced Vacuum Technology reducing signal-to-noise ratio of 200:1 for the vacuum system for improved sensitivity the pressure in the ultra-high vacuum transition of the isolated protonated and desolvation 10 region to <2 × 10 and enhancing molecular ions at m/z 609 to the largest two • Vent-free maintenance transmission of the ions to the Orbitrap mass analyzer product ions, m/z 397 and m/z 448, when Electrodynamic Ion Funnel the mass spectrometer is operated at unit Options • EDIF, a radio frequency (RF) device, resolution in the full scan MS/MS mode, m/z efficiently captures ions as they leave Thermo Scientific™ NanoSpray Flex NG™ 165–615. The test requires HESI III probe. the HCTT Ion Source Dynamic Range • Automatic tuning results in broad • Supports static and dynamic > 5,000 within a single OTMS spectrum transmission curves and reduces ion nanoelectrospray experiments losses, increasing sensitivity • Compatible with liquid flow rates of MS Scan Power 50 nL/min to 2 μL/min Advanced Active Beam Guide MSn, for n = 1 through 10 • Advanced Active Beam Guide with an axial ™ ™ Thermo Scientific EASY-Spray Synchronous Precursor Isolation field and low pass filtering reduces noise Ion Source by preventing neutrals and high-velocity Up to 20 precursors per MSn scan clusters from entering the quadrupole • Maximum nanoelectrospray performance mass filter with no need for adjustments Multiplexing Using the Ion Routing Multipole Thermo Scientific™ EASY-ETD Ion Source Advanced Quadrupole Technology Up to 10 precursors per scan using the • Segmented Quadrupole Mass Filter for • Improved dynamic range and S/N for ™ quadrupole mass filter precursor ion selection with variable ETD fragments using Easy ETD HD precursor isolation width from 0.4 u • Generates fluoranthene anions for electron Polarity Switching to 1,200 u transfer dissociation (ETD) One full cycle in < 1.1 sec (one full scan • Superior ion transmission across isolation • Townsend discharge provides extremely positive mode and one full scan negative widths up to 1,200 u stable and robust ionization mode at resolution setting of 30,000) • MS/MS precursor ion selection with high • Compact size, located entirely within the transmission from 50 to 3,000 m/z footprint of the instrument ETD Efficiency • Active reagent ion filtering using the ETD fragmentation efficiency of >15%, Ion-Routing Multipole quadrupole mass filter (infusion of 1 pmol/μL angiotensin at 3 μL/min) • IRM for ion storage • The ion sorting routines and charge state • Higher-energy collisional dissociation dependent calibration of the ETD reaction Analog Inputs (HCD) cell time maximize ETD spectral quality Channel 1 analog input (0–10 V), • Efficient, reliable ion transfer between the ™ ™ Channel 2 analog (0–2 V) ion-routing multipole and the Orbitrap and Thermo Scientific EASY-IC Ion Source linear ion trap mass analyzers • Generates internal calibrant ions for *Under defined conditions real-time mass calibration of MS scans Orbitrap Mass Analyzer in both positive and negative modes • Next-generation, ultra high-field Orbitrap • Provides <1 ppm RMS mass analyzer accuracy under defined conditions • New low noise detection pre-amplifier and minimizes effort 3 Software Features Exclusive Technologies • Synchronous MS3 precursor selection • Dynamic Scan Management allows significantly increases the number of Data System peptides accurately quantified in isobaric ® for intelligent, real-time scheduling, • High-performance PC with Intel parallelization, and prioritization of the mass tagging experiments microprocessor scan events. It also enables selection, • Data-dependent neutral-loss experiment • High-resolution LCD color monitor sorting, and routing of precursors to triggers an MS3 scans only for product ions • Microsoft® Windows® 7 operating system different fragmentation modes and with a predefined neutral loss analyzers based on user-selected • Microsoft Office 2010 software • Isolation offset allows for custom centering parameters, including precursor m/z, of the window when working with broad • Thermo Scientific™ Xcalibur™ processing intensity, and/or charge isotopic distributions and instrument control software • Precursor fragmentation can take place • Quanfirmation experiment uses • Tune editor for system calibration, in the ion-routing multipole (HCD), in the synchronous HRAM SIM for quantitation diagnostics, and manual data acquisition ion trap (CID, ETD) or both (EThcD) with with full-scan MS/MS in the linear ion trap • Method editor with comprehensive fragment detection in the linear ion trap or for precursor identity confirmation application-specific template library and Orbitrap mass analyzers at any stage of drag-and-drop user interface to facilitate MSn analysis Optional Application-Specific Software method development • All Dynamically Available Parallelizable • Thermo Scientific™ Proteome Discoverer™ Time (ADAPT™) technology determines in software—flexible, expandable platform Operation Modes real time the most efficient parallelization