Nano-computed Tomography (nano-CT)
• Nano-computed Tomography (nano-CT)
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• Nano-computed Tomography (nano-CT)
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• Nano-computed Tomography (nano-CT)
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Tiger moth – Melese
Iso-surface rendering Tiger moth – Melese
Iso-surface rendering Diagnostic structures in situ
Accurate measurements
Type specimens
Really small specimens Diagnostic structures in situ
Accurate measurements
Type specimens
Really small specimens
Peach fruit moth - Carposina sasakii
1.0 mm • •
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DoodleBug Biology (@DoodleBugBio) Evolution of defensive ultrasound production in moths
Kawahara & Breinholt (2014) Evolution of defensive ultrasound production in moths
• Evolution of defensive ultrasound production in moths
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0.5 mm Pyralidae/Crambidae– dorsal thoracic scales
Axel Hausmann, SNSB (2010)
Barber et al. (in prep.) Pyralidae/Crambidae– dorsal thoracic scales
Barber et al. (in prep.) Erebidae: Calpinae – ventral abdominal scales
0.5 mm
50 μm • •
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MAYBE!!! Marthula (Noctuoidea: Notodontidae)
Abdomen moves during ultrasound production, but no correlated structures observed with regular microscopy or SEM
Dan Janzen (2008) Notodontidae – Marthula
Iso-surface rendering
Ommatidia Flight muscles
Gut contents
Notodontidae – Marthula
Volumetric rendering Search for less dense cuticle – possible evidence of ultrasound production Search for less dense cuticle – possible evidence of ultrasound production Nano-CT offers new avenues for exploring insect biodiversity and morphological variation
Useful tool for developing new hypotheses
Dan Janzen (2008) Kawahara & Breinholt (2014) Kawahara & Breinholt (2014)
Acknowledgments
The Kawahara Lab FLMNH Jesse Breinholt David Blackburn Geena Hill Andrei Sourakov Nicholas Homziak Lary Reeves https://www.floridamuseum.ufl.edu/mcguire/kawahara/
The Barber Lab UF Nanoscale Research Facility Brian Leavell Gary Scheiffele Krystie Miner Edward Stanley
Florida Department of Agriculture – Division of Plant Industry Dalceridae photograph by Ken-Ichi Ueda (2013) - https://www.flickr.com/photos/ken- This research was funded by NSF grants CSBR- ichi/10769397635/in/photolist-hpDYHz- 1349345, IOS-1121739, and IOS -1121807 ciJHT5-qusukF-qF42Bd-EHjKeu Creative Commons license: https://creativecommons.org/licenses/by/2.0/